JPH0521014Y2 - - Google Patents

Description

[Detailed explanation of the idea]

(Industrial Application Field) The present invention relates to a multilayer analytical element for chemical analysis, in particular,
This invention relates to a multilayer analytical element in which an adhesive tape is provided on a spreading layer. (Prior Art) Automatic quantitative analyzers such as flow type and discrete type are used for quantitative analysis of liquid samples of biological origin such as blood and urine. Although this device has high analytical throughput, it requires warming up before use and cleaning after use. There is also the problem of environmental pollution in the treatment of cleaning waste liquid. Another problem is that the device is expensive and requires skill to operate. In order to solve these drawbacks, a method for quantitatively analyzing a liquid sample of biological origin using an optical method using a multilayer analytical element has been proposed. What is a multilayer analysis element?
It is a material for quickly and easily quantifying specific chemical components in liquid samples of biological origin using a dry method.
No. 40191, JP 51-40191, JP 52-
It is disclosed in JP-A No. 131786, JP-A-53-89796, and JP-A-55-26428. A liquid sample of biological origin is placed on this multilayer analysis element, and the change in color or concentration is determined visually or by reflection photometry, thereby quantitatively analyzing the component to be detected in the sample. However, in this multilayer analytical element, since the developing layer is in direct contact with the outside air, components in the reagent layer absorb moisture, oxidize, or volatilize through the developing layer, resulting in deterioration of the reagent layer. There is also a possibility that the expansion layer may be damaged due to physical causes. As a result, analysis accuracy is seriously hindered. (Problems to be solved by the invention) The present invention solves the above-mentioned conventional problems, and its purpose is to provide a multilayer analytical element in which the reagent layer undergoes little deterioration during storage or reaction. There is a particular thing. Another object of the present invention is to provide a multilayer analytical element whose spreading layer is not damaged during storage or reaction. (Means for Solving the Problems) The present invention provides an analytical element having at least a reagent layer and a developing layer sequentially laminated on at least a portion of a light-transmitting support. A tape is installed on the side opposite to the reagent layer with respect to the spreading layer via a second adhesive tape, the second adhesive tape has a sample spotting hole in a part, and the second adhesive tape The first adhesive tape is bonded to the support and/or the spreading layer, and the spreading layer and the first spreading layer are bonded to each other in the sample spotting hole.
The adhesive tape is a multilayer analysis element characterized by being spaced apart from each other to form a space, and by having such a configuration, the above object is achieved. The light-transmitting support is, for example, a medium for transmitting irradiated light from a spectrophotometer, detecting the reflected light by an appropriate means, and measuring a change in color of the reagent layer. Any light-transparent and liquid-impermeable material can be used for this support. Examples include polyethylene terephthalate (PET), cellulose triacetate, polycarbonate, polyvinyl chloride, polystyrene, polymethyl methacrylate,
There's glass. Support thickness is 50μm to 2000μm
is suitable. However, materials with low fluorescence radiation transmission, such as polycarbonate, cellulose triacetate, and polystyrene, are preferred for supports used in elements for fluorescence measurement. The reagent layer is a layer containing a reagent that changes the color of a test sample, for example, and is prepared by mixing a hydrophilic polymer with at least one of a reagent and an enzyme. Hydrophilic polymers include gelatin, pullulan, sodium agarose alginate, water-soluble hydroxyethyl celluloses, polyacrylamide, polyvinyl alcohol, polyvinylpyrrolidone, acrylic copolymers, and the like. The spreading layer is a layer for uniformly diffusing the spotted sample and supplying it to the reagent layer. For example, membrane filters, cloth, filter paper, non-woven fabrics,
Glass fiber etc. are used. However, in the case of protein quantification, protein-adsorbing materials such as nitrocellulose are not preferred. Adhesive tape (this will be the first adhesive tape)
is adhered and fixed to the support and/or the spreading layer via another second adhesive tape. This second adhesive tape is applied by punching or other means.
A hole is provided for a sample point. There is no limit to the number of holes, and it is also possible to provide multiple holes independently from each other in order to measure multiple samples simultaneously. The spreading layer located at the sample spotting hole is located with a gap in between and the first adhesive tape so as not to come into contact with the first adhesive tape. Any known adhesive tape can be used for the first and second adhesive tapes. Since the first adhesive tape only needs to be easily peeled off from the element, the adhesion force of the first adhesive tape to the second adhesive tape is limited to the support of the second adhesive tape and/or The adhesive force is adjusted so that it is lower than the adhesive force to the spreading layer. Adjustment of adhesive strength includes, for example, backside treatment such as silicone treatment. The second adhesive tape is provided with at least one sample spot hole by a conventional punching process. In addition to the reagent layer, an extraction layer and a light blocking layer may be provided as appropriate between the support and the development layer of the multilayer analytical element, depending on each reaction. The extraction layer is, for example, a layer for extracting a reactant in an antigen-antibody reaction, and an example thereof is the hydrophilic polymer used in the reagent layer. The light-blocking layer is a layer that blocks light irradiated from a spectrophotometer and transmitted through a light-transmitting support, and is made by adding titanium oxide and/or barium sulfate to the hydrophilic polymer, for example, from 1 to 30% by weight ( (for polymers). A surfactant may be added to the multilayer analytical element, if necessary. Surfactants facilitate the movement of sample components within the device. Examples include nonionic surfactants such as polyoxyethylene, alkyl allyl ethers of polyglycerol, sorbitan esters, fatty acid esters. (Example) The present invention will be described below with reference to an example. Example 1 In the multilayer analytical element of the present invention, as shown in FIG. 1, a second adhesive tape 5 having a hole 50 is pasted on a support 1. A reagent layer 2 is placed inside this hole 50.
A spreading layer 3 is provided on the reagent layer 2. A first adhesive tape 4 is disposed on the spreading layer 3 and is bonded to a second adhesive tape 5.
The first adhesive tape 4 and the spreading layer 3 are arranged so that a gap 51 is formed therebetween. This analytical element is manufactured, for example, as follows. Polyethylene terephthalate film (thickness 4
Adhesive tape (adhesive layer 30μm, polyester base layer) with a circular shape of 10mmφ punched out in the center on
500μm) was attached. The sample solution (copper sulfate 1.68 mg, neocuproine 2.56 mg,
7.85μ of gelatin (110 mg, water 10 ml) was injected and developed, and dried at room temperature to form a reagent layer. Millipore MF membrane (9mmφ, thickness 180μm, average pore diameter 1.2μ
m) was moistened, brought into close contact with this reagent layer, and after applying light pressure, was dried to form a spreading layer. In addition to the adhesive tape, another adhesive tape (adhesive layer 25 μm,
A polyester base layer (25 μm) was attached. Example 2 Another multilayer analytical element of the present invention is shown in FIG. In the device in this case, a reagent layer 2 is provided on a support 1, and a spreading layer 3 is provided on the reagent layer 2. A second adhesive tape 5 having holes 50 is disposed on the spreading layer 3.
A first adhesive tape 4 is disposed through it.
This element is manufactured, for example, as follows. Polyethylene terephthalate film (thickness 4
Mill, 12.5 x 12.5 mm), place the sample solution (copper sulfate 1.68
mg, neocuproine 2.56mg, gelatin 110mg, water
10ml) 15.7μ was injected and developed, and dried at room temperature to form a reagent layer. Millipore MF membrane (thickness
180 μm, average pore diameter 1.2 μm, 12.5×12.5 mm) was moistened, brought into close contact with this reagent layer, and dried while applying light pressure to form a spread layer. Next, an adhesive tape with a circular shape of 10 mm diameter punched out in the center was pasted onto this spreading layer, and another adhesive tape was then pasted on top of it. Example 3 Polyethylene terephthalate film (thickness 4
mill) on top of the sample solution (glucose oxidase
21.5mg, peroxidase 10.7mg, O-dianisidine hydrochloride 32.0mg, gelatin 2150mg, water 100ml)
15.7μ was injected and developed, and dried at room temperature to form a reagent layer. On this reagent layer, a 180 μm thick MF membrane was provided using the same method as in Example 2 to form a spreading layer. An adhesive tape with a circular shape of 10 mmφ punched out in the center was pasted on the development layer, and another adhesive tape was further pasted on top of it. Peel off the adhesive tape on the outside of this multilayer analytical element and place a 10μ
When the adhesive tape was applied again after spotting the whole blood sample (samples No. 1 to 5 of this invention),
The reflection density of the peeled samples (control samples Nos. 6 to 10) 10 minutes after spotting was measured. The results are shown in the table below.

[Table] As is clear from the table, there is less variation in the measured values of reflection density when the peeled adhesive tape is reapplied and reacted than when it is left peeled. As a result, the multilayer analysis element of the present invention, which has two layers of adhesive tape, enables more accurate analysis than the control element. (Effects of the Invention) Since the multilayer analytical element of the present invention has the adhesive tape removably provided on the spreading layer, there is no deterioration of the reagent layer and no damage to the spreading layer during storage. As a result, analysis accuracy improves. Further, even during the reaction, by re-installing the peeled adhesive tape, volatilization of reagents, evaporation of water, etc. are prevented, and analysis accuracy is further improved.

[Brief explanation of the drawing]

FIG. 1 is a front sectional view showing one embodiment of the multilayer analysis element of the present invention, and FIG. 2 is a front sectional view showing another embodiment of the multilayer analysis element of the invention. 1... Light-transparent support, 2... Reagent layer, 3...
Developing layer, 4...first adhesive tape, 5...second adhesive tape, 50...sample spotting hole.

Claims (1)

[Claims for Utility Model Registration] 1. In an analytical element having at least a part of a light-transmitting support in which at least a reagent layer and a developing layer are sequentially laminated, a first removable adhesive tape is attached to a second removable adhesive tape. The second adhesive tape is placed on the side opposite to the reagent layer with respect to the developing layer through an adhesive tape, and the second adhesive tape has a sample spotting hole in a part thereof, and the second adhesive tape has a sample spotting hole in a part thereof. The adhesive tape and the support and/or the spreading layer are adhered, and the spreading layer and the first adhesive tape are located apart from each other in the sample spotting hole to form a space. A multilayer analytical element featuring: 2. A utility model registration claim characterized in that the adhesive force of the first adhesive tape to the second adhesive tape is lower than the adhesive force of the second adhesive tape to the support and/or the spreading layer. A multilayer analytical element according to scope 1.