CN115436459A - Automatic capillary needle replacement device and method - Google Patents
Automatic capillary needle replacement device and method Download PDFInfo
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- CN115436459A CN115436459A CN202210370624.7A CN202210370624A CN115436459A CN 115436459 A CN115436459 A CN 115436459A CN 202210370624 A CN202210370624 A CN 202210370624A CN 115436459 A CN115436459 A CN 115436459A
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- 238000000034 method Methods 0.000 title claims abstract description 17
- 239000004020 conductor Substances 0.000 claims abstract description 28
- 238000007789 sealing Methods 0.000 claims abstract description 14
- 239000011159 matrix material Substances 0.000 claims description 6
- 230000003247 decreasing effect Effects 0.000 claims 1
- 238000012864 cross contamination Methods 0.000 description 4
- 238000001514 detection method Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 241000282414 Homo sapiens Species 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000013459 approach Methods 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 238000004949 mass spectrometry Methods 0.000 description 2
- 241000282412 Homo Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000002146 bilateral effect Effects 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000010417 needlework Methods 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000001225 therapeutic effect Effects 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
- G01N27/62—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode
- G01N27/68—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating the ionisation of gases, e.g. aerosols; by investigating electric discharges, e.g. emission of cathode using electric discharge to ionise a gas
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- Analytical Chemistry (AREA)
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides a device and a method for automatically replacing a capillary needle, wherein the device for automatically replacing the capillary needle comprises: the clamping unit comprises a base, a first clamping jaw, a second clamping jaw, a guide rail and a driving unit, wherein the first clamping jaw and/or the second clamping jaw are/is arranged on the guide rail, the guide rail is arranged on the base, and the driving unit is used for driving the clamping jaws on the guide rail to move forwards and backwards; the first clamping jaw and the second clamping jaw are provided with oppositely arranged grooves allowing the capillary needles to enter, and the distance between the bottom walls of the grooves at opposite positions is increased from bottom to top; the interface unit is fixed on the base and is positioned between the first clamping jaw and the second clamping jaw; the interface unit is provided with a gas channel, an electric conductor is connected with an elastic piece and arranged in the gas channel, and a first sealing piece is arranged on the electric conductor and realizes the opening and closing of the opening end of the gas channel along with the movement of the electric conductor. The invention has the advantages of accurate replacement and the like.
Description
Technical Field
The invention relates to cell analysis, in particular to an automatic capillary needle replacement device and method.
Background
The cell is an important research object for human to research life secret and explain disease mechanism. Traditional cell biology studies often target population cells, and have explored the pathogenesis and therapeutic approaches of many diseases for humans. But there are significant drawbacks based on the average study of the population cells, ignoring cellular "heterogeneity". The single cell analysis can make up for the important information which is covered and omitted in the past due to the sampling of the group cells, so that the research of human beings on diseases is more accurate and comprehensive. The single cell mass spectrometry technology can realize simultaneous detection of multi-component samples, obtain structural information of unknown components in a large number of samples, has higher sensitivity, and provides effective technical support for researches of single cell metabonomics, proteomics and the like.
The defects of the current single-cell mass spectrometry technology mainly lie in that:
1. the capillary needle runs through the whole process for use, so that the potential risk of cross contamination is high, and the scheme based on the capillary needle has the problem of cross contamination among single cells;
some schemes introduce a capillary needle cleaning technology, but the system working process becomes more complicated, and the reliability is reduced.
2. The bilateral capillary needles are operated cooperatively, even a rotary motor is possibly required to intervene, the control is complex, and errors are easy to occur.
Disclosure of Invention
In order to overcome the defects in the prior art, the invention provides an automatic capillary needle replacing device.
The purpose of the invention is realized by the following technical scheme:
an automated capillary needle exchange device, comprising:
the clamping unit comprises a base, a first clamping jaw, a second clamping jaw, a guide rail and a driving unit, wherein the first clamping jaw and/or the second clamping jaw are/is arranged on the guide rail, the guide rail is arranged on the base, and the driving unit is used for driving the clamping jaws on the guide rail to move forwards and backwards; the first clamping jaw and the second clamping jaw are provided with oppositely arranged grooves allowing the capillary needles to enter, and the distance between the bottom walls of the grooves at opposite positions is increased from bottom to top;
the interface unit is fixed on the base and is positioned between the first clamping jaw and the second clamping jaw; the interface unit is provided with a gas channel, an electric conductor is connected with an elastic piece and arranged in the gas channel, and a first sealing piece is arranged on the electric conductor and realizes the opening and closing of the opening end of the gas channel along with the movement of the electric conductor.
Another object of the present invention is to provide a capillary needle combined with an automatic changing device, which is achieved by the following technical solutions:
a capillary needle comprising a capillary tube; the capillary needle further comprises:
a mount having a channel allowing the capillary tube to pass through, the mount comprising a reduced outer diameter section;
the discharge needle is arranged on the fixing piece, penetrates through the channel and is inserted into the capillary; a gap is arranged between the channel and the discharge needle.
The invention also aims to provide an automatic capillary needle replacement method, which is realized by the following technical scheme:
the automatic replacement method of the capillary needle comprises the following steps:
the clamping unit moves to the capillary needle;
the driving unit drives the first clamping jaw and/or the second clamping jaw on the guide rail to move, and the capillary needle is clamped by the grooves on the first clamping jaw and the second clamping jaw at opposite positions and moves forwards in the grooves towards the interface unit;
the discharge needle of the capillary needle pushes against the electric conductor in the interface unit gas channel to move forward, the elastic part connected with the electric conductor deforms, the opening end of the gas channel is opened, and the gas channel is communicated with the inside of the capillary needle;
after the capillary needle is used, the driving unit drives the first clamping jaw and/or the second clamping jaw on the guide rail to move, the grooves in opposite positions are separated, the elastic piece drives the electric conductor to move reversely, the opening end is closed, and the capillary needle moves reversely and is separated from the grooves.
Compared with the prior art, the invention has the beneficial effects that:
1. cross contamination is avoided;
the capillary needle bearing unit is provided with a plurality of capillary needles which are distributed in a matrix form, for example, in each single cell detection, a new capillary needle is firstly clamped and used for other work, such as single cell extraction and detection, and the capillary needles are used as consumable materials, so that the cross contamination among the capillary needles is avoided;
2. the structure is simple;
at the same time, only one capillary needle works, namely only one three-dimensional moving unit is needed to realize the three-dimensional movement of the capillary needle, so that the subsequent operations such as single cell extraction and detection are realized, other control parts are not needed, the complexity of the system is reduced, and the reliability is improved;
due to the design of the interface unit, the air circuit and the circuit are connected and disconnected simultaneously, and the structural complexity is reduced;
3. the replacement efficiency is high;
the loading and the recovery of the capillary needle are realized automatically, so that the replacement efficiency is obviously improved;
the outer diameter of each section of the capillary needle is designed in a variable mode, and the grooves matched with the outer diameter of each section of the capillary needle are designed, so that the capillary needle is clamped by the first clamping jaw and the second clamping jaw quickly and accurately, meanwhile, the conduction of the air circuit and the electric circuit is achieved, and the overall working efficiency is improved.
Drawings
The present disclosure will become more readily understood with reference to the accompanying drawings. As is readily understood by those skilled in the art: these drawings are only for illustrating the technical solution of the present invention and are not intended to limit the scope of the present invention. In the figure:
FIG. 1 is a schematic structural view of an apparatus for automatically replacing a capillary needle according to an embodiment of the present invention;
fig. 2 is a schematic structural view of a capillary needle according to an embodiment of the present invention.
Detailed Description
Fig. 1-2 and the following description depict alternative embodiments of the invention to teach those skilled in the art how to make and use the invention. Some conventional aspects have been simplified or omitted for the purpose of explaining the technical solution of the present invention. Those skilled in the art will appreciate that variations or substitutions from these embodiments will be within the scope of the invention. Those skilled in the art will appreciate that the features described below can be combined in various ways to form multiple variations of the invention. Thus, the present invention is not limited to the following alternative embodiments, but is only limited by the claims and their equivalents.
Example 1:
fig. 1 is a schematic view showing a configuration of an automatic capillary needle replacement device according to embodiment 1 of the present invention, and as shown in fig. 1, the automatic capillary needle replacement device includes:
the clamping device comprises a clamping unit 1, a first clamping jaw 12, a second clamping jaw 13, a guide rail and a driving unit, wherein the clamping unit comprises a base 11, the first clamping jaw 12, the second clamping jaw 13, the guide rail and the driving unit, the first clamping jaw 12 and/or the second clamping jaw 13 are/is arranged on the guide rail, the guide rail is arranged on the base 11, and the driving unit is used for driving the clamping jaws on the guide rail to move forwards and backwards; the first clamping jaw 12 and the second clamping jaw 13 are provided with oppositely arranged grooves 14-15 allowing the capillary needle 2 to enter, and the distance between the bottom walls of the oppositely arranged grooves 14-15 is increased from bottom to top;
the interface unit 3 is fixed on the base 11 and is positioned between the first clamping jaw 12 and the second clamping jaw 13; the interface unit 3 is provided with a gas channel, an electric conductor 31 is connected with an elastic piece and arranged in the gas channel, a first sealing piece is arranged on the electric conductor 31, and the opening and the closing of the opening end of the gas channel are realized along with the movement of the electric conductor 31.
In order to realize the sealing between the interface unit 3 and the capillary needle 2, further, the interface unit 3 further includes:
a second seal 32, said second seal 32 surrounding said open end.
In order to improve the clamping efficiency of the capillary needle 2, the first clamping jaw 12 and the second clamping jaw 13 are respectively arranged on the guide rails and driven by the driving unit to move towards opposite directions, such as towards each other when clamping the capillary needle 2 and away from each other when discarding the capillary needle 2.
In order to store the capillary needles 2, further, the automatic changing device of the capillary needles 2 further comprises:
the bearing unit is provided with a plurality of capillary needle bearing positions distributed in a matrix form;
the moving unit is used for realizing relative three-dimensional movement between the bearing unit and the clamping unit;
and the second magnetic parts are arranged on the bearing positions.
Fig. 2 schematically shows a structural view of a capillary needle 2 of embodiment 1 of the present invention, which, as shown in fig. 2, includes:
a capillary 23, such as a glass capillary;
a mount 24, said mount 24 having a passage 20 allowing said capillary to pass through, said mount 24 comprising a reduced outer diameter section 21;
a discharge needle 25, wherein the discharge needle 25 is arranged on the fixing member 24, passes through the channel 20 and is inserted into the capillary 23; a gap is formed between the channel 20 and the discharge needle 25.
In order to accurately and rapidly clamp the capillary needle 2, further, the fixing member 24 further includes an outer diameter-increased section 22, and an outer diameter-unchanged section 27 is arranged between the outer diameter-decreased section 21 and the outer diameter-increased section 22.
The automatic replacement method of the capillary needle according to the embodiment of the present invention, that is, the working method of the replacement device according to the embodiment of the present invention, includes:
the clamping unit 1 moves to the capillary needle 2;
the driving unit drives the first clamping jaw 12 and/or the second clamping jaw 13 on the guide rail to move, the capillary needle 2 is clamped by the grooves 14-15 on the first clamping jaw 12 and the second clamping jaw 13, which are opposite in position, and moves forwards in the grooves 14-15 towards the interface unit, namely, along with the clamping of the grooves 14-15, the movement in one direction between the grooves is converted into the movement in the other direction of the capillary needle 2, and an included angle between the two directions is a right angle;
the discharge needle 25 of the capillary needle 2 pushes against the electric conductor 31 in the air channel of the interface unit 3 to move forward, the elastic piece connected with the electric conductor 31 deforms, the open end of the air channel is opened, and the air channel is communicated with the interior of the capillary needle 2;
after the capillary needle 2 is used, the driving unit drives the first clamping jaw and/or the second clamping jaw on the guide rail to move, the grooves 14-15 which are opposite in position are separated, the elastic piece drives the electric conductor 31 to move reversely, the open end is closed, and the capillary needle 2 moves reversely and is separated from the grooves 14-15.
In order to reserve a plurality of capillary needles 2 for replacement, furthermore, a plurality of capillary needles 2 are arranged in a matrix on a carrier unit,
three-dimensional movement occurs between the carrier unit and the gripper unit 1, such that the gripper unit 1 moves to the selected capillary needle 2.
In order to fix the capillary needle 2, a first magnetic part 29 is arranged on the capillary needle 2, and a second magnetic part is arranged at the capillary needle bearing position of the bearing unit.
To match with the gripper unit 1, further, as shown in fig. 2, the capillary needle 2 includes a capillary 23, a holder 24, and a discharge needle 25, the holder 23 having a passage 20 allowing the capillary 23 to pass through, the holder 24 including a reduced outer diameter section 21; the discharge needle 25 is arranged on the fixing member 24, passes through the channel 20 and is inserted into the capillary 23; a gap is formed between the channel 20 and the discharge needle 25.
Example 2:
an example of application of the automatic capillary needle replacement device and the operation method thereof according to embodiment 1 of the present invention.
In the present application example, as shown in fig. 1, in the capillary needle changer, the first jaw 12 and the second jaw 13 are both disposed on the guide rail and are moved in opposite directions, i.e., moved toward and away from each other, by the driving unit; the first clamping jaw 12 is provided with a groove 14, the second clamping jaw 13 is provided with a groove 15, the openings of the two grooves 14-15 are opposite, and the distance between the bottom walls of the two grooves 14-15 is increased from bottom to top and then is kept unchanged;
in the interface unit 3, a conductor 31, an elastic member, a first sealing member and a second sealing member 32 are arranged in the gas channel, the first sealing member is sleeved on the conductor 31, the elastic member adopts a spring, one end of the elastic member is connected with the conductor 31, and under the action of the elastic member, the first sealing member is contacted with the opening end of the gas channel, so that the opening end is closed; when the conductor 31 is driven by an external force, the elastic member is further compressed, the first sealing member is separated from the open end, and the open end is opened; a second seal 32 is disposed around the open end;
the bearing unit is provided with a plurality of capillary needle bearing positions distributed in a matrix form;
the moving unit is used for realizing relative three-dimensional movement between the bearing unit and the clamping unit, so that the clamping unit corresponds to the capillary needle 2 selected on the bearing unit;
a plurality of second magnetic parts, which are arranged at the bearing position and are matched with the first magnetic parts 29 on the capillary needle 2, so as to fix the capillary needle 2.
As shown in fig. 2, the capillary needle 2 includes: a glass capillary tube; the fixing member 24 has a passage 20 for allowing the capillary tube to pass through, and the fixing member 24 includes an outer diameter-unchanged section 26, an outer diameter-reduced section 21, an outer diameter-unchanged section 27, and an outer diameter-increased section 22; the discharge needle 25 is arranged on the fixing piece 24, one end of the discharge needle is spherical, and the other end of the discharge needle penetrates through the channel 20 and is inserted into the capillary 23; a gap is arranged between the channel 20 and the discharge needle 25; the first magnetic part 29 is provided with a connecting and fixing part 24 and is provided with a channel for the capillary 23 to pass through; by means of the design of the capillary needle external diameter constant section 26 and the external diameter small section 21 and the arrangement of the matched distance between the two grooves 14-15, the two grooves 14-15 clamp the capillary needle 2 and realize the movement of the capillary needle 2, and the capillary needle 2 is in contact with the interface unit to extrude the second sealing element 32.
The automatic replacement method of the capillary needle according to the embodiment of the present invention, that is, the working method of the replacement device according to the embodiment of the present invention, includes:
the moving unit works to enable the clamping unit 1 to correspond to the selected capillary needle 2 on the bearing unit, and the first clamping jaw 12 and the second clamping jaw 13 are positioned at two sides of the selected capillary needle 2;
the driving unit drives the first clamping jaw 12 and the second clamping jaw 13 on the guide rail to approach in the horizontal direction, the bottom ends of the grooves 14-15 push the outer diameter small sections 21 of the capillary needles 2, so that the capillary needles 2 vertically move upwards, and the outer diameter constant sections 26 and the outer diameter small sections 21 of the capillary needles 2 enter the grooves 14-15;
the top end of the capillary needle 2 moving upwards extrudes the second sealing element 32 to realize the sealing between the capillary needle 2 and the interface unit 1, the spherical end of the discharge needle 25 pushes the electric conductor 31 to move upwards, so that the first sealing element is separated from the open end, the open end is opened, the gas channel is communicated with the channel 20 of the capillary needle 2, and the simultaneous communication of a circuit and gas is realized;
after the capillary needle 2 is used, the driving unit drives the first clamping jaw and the second clamping jaw on the guide rail to move, the grooves 14-15 in opposite positions are separated, the elastic piece drives the electric conductor 31 to move reversely, the capillary needle 2 is separated from the interface unit 3, the opening end is closed, the capillary needle 2 moves reversely and is separated from the discharge needle 25 and the electric conductor 31 of the grooves 14-15, and therefore the air path and the circuit are disconnected.
Claims (10)
1. Automatic exchange device of capillary needle, characterized in that, automatic exchange device includes:
the clamping unit comprises a base, a first clamping jaw, a second clamping jaw, a guide rail and a driving unit, wherein the first clamping jaw and/or the second clamping jaw are/is arranged on the guide rail, the guide rail is arranged on the base, and the driving unit is used for driving the clamping jaws on the guide rail to move forwards and backwards; the first clamping jaw and the second clamping jaw are provided with oppositely arranged grooves allowing the capillary needles to enter, and the distance between the bottom walls of the grooves at opposite positions is increased from bottom to top;
the interface unit is fixed on the base and is positioned between the first clamping jaw and the second clamping jaw; the interface unit is provided with a gas channel, the electric conductor is connected with the elastic piece and arranged in the gas channel, the first sealing piece is arranged on the electric conductor, and the opening and the closing of the opening end of the gas channel are realized along with the movement of the electric conductor.
2. The automated capillary needle exchange device of claim 1, wherein the interface unit further comprises:
a second seal surrounding the open end.
3. The apparatus according to claim 1, wherein the first and second jaws are respectively provided on the guide rail to be moved in opposite directions by the driving unit.
4. The apparatus for automatically exchanging a capillary needle according to claim 1, further comprising:
the bearing unit is provided with a plurality of capillary needle bearing positions distributed in a matrix form;
the moving unit is used for realizing relative three-dimensional movement between the bearing unit and the clamping unit;
and the second magnetic parts are arranged on the bearing positions.
5. A capillary needle comprising a capillary tube; it is characterized in that the capillary needle further comprises:
a mount having a channel allowing the capillary tube to pass through, the mount comprising a reduced outer diameter section;
the discharge needle is arranged on the fixing piece, penetrates through the channel and is inserted into the capillary; a gap is arranged between the channel and the discharge needle.
6. The capillary needle according to claim 5, wherein the fixture further comprises an outer diameter increasing section, and an outer diameter constant section is arranged between the outer diameter decreasing section and the outer diameter increasing section.
7. The automatic replacement method of the capillary needle comprises the following steps:
the clamping unit moves to the capillary needle;
the driving unit drives the first clamping jaw and/or the second clamping jaw on the guide rail to move, and the capillary needle is clamped by the grooves on the first clamping jaw and the second clamping jaw at opposite positions and moves forwards in the grooves towards the interface unit;
the discharge needle of the capillary needle pushes against the electric conductor in the interface unit gas channel to move forwards, the elastic part connected with the electric conductor deforms, the opening end of the gas channel is opened, and the gas channel is communicated with the inside of the capillary needle;
after the capillary needle is used, the driving unit drives the first clamping jaw and/or the second clamping jaw on the guide rail to move, the grooves in opposite positions are separated, the elastic piece drives the electric conductor to move reversely, the opening end is closed, and the capillary needle moves reversely and is separated from the grooves.
8. The method of claim 7, wherein a plurality of the capillary needles are arranged on the carrier unit in a matrix form,
three-dimensional movement occurs between the bearing unit and the clamping unit, so that the clamping unit moves to the selected capillary needle.
9. The method for automatically replacing the capillary needle as claimed in claim 8, wherein a first magnetic attraction part is arranged on the capillary needle, and a second magnetic attraction part is arranged at the capillary needle bearing position of the bearing unit.
10. The method of claim 7, wherein the capillary needle comprises a capillary tube, a holder having a passage allowing the capillary tube to pass therethrough, and a discharge needle, the holder comprising a reduced outer diameter section; the discharge needle is arranged on the fixing piece, penetrates through the channel and is inserted into the capillary; a gap is arranged between the channel and the discharge needle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210370624.7A CN115436459A (en) | 2022-04-10 | 2022-04-10 | Automatic capillary needle replacement device and method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210370624.7A CN115436459A (en) | 2022-04-10 | 2022-04-10 | Automatic capillary needle replacement device and method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115436459A true CN115436459A (en) | 2022-12-06 |
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ID=84241677
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210370624.7A Pending CN115436459A (en) | 2022-04-10 | 2022-04-10 | Automatic capillary needle replacement device and method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115436459A (en) |
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2022
- 2022-04-10 CN CN202210370624.7A patent/CN115436459A/en active Pending
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Country or region after: Zhong Guo Address after: West side of 1st floor, 1st floor, Building A, No. 288 Jingu Middle Road (East), Yinzhou District, Ningbo City, Zhejiang Province, 315000 Applicant after: CHINA INNOVATION INSTRUMENT Co.,Ltd. Address before: Room 304, D Building, Kexin Building, 655 Xueshi Road, Yinzhou District, Ningbo City, Zhejiang Province, 315000 Applicant before: CHINA INNOVATION INSTRUMENT Co.,Ltd. Country or region before: Zhong Guo |
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