CN115418406B - 用于预测肺炎克雷伯菌对抗生素药敏表型的特征基因组合、试剂盒及测序方法 - Google Patents
用于预测肺炎克雷伯菌对抗生素药敏表型的特征基因组合、试剂盒及测序方法 Download PDFInfo
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- CN115418406B CN115418406B CN202211147291.8A CN202211147291A CN115418406B CN 115418406 B CN115418406 B CN 115418406B CN 202211147291 A CN202211147291 A CN 202211147291A CN 115418406 B CN115418406 B CN 115418406B
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- 239000003814 drug Substances 0.000 title claims abstract description 71
- 229940079593 drug Drugs 0.000 title claims abstract description 67
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 57
- 241000588747 Klebsiella pneumoniae Species 0.000 title claims abstract description 53
- 230000035945 sensitivity Effects 0.000 title claims abstract description 30
- 238000012163 sequencing technique Methods 0.000 title abstract description 15
- 239000003242 anti bacterial agent Substances 0.000 title abstract description 12
- 229940088710 antibiotic agent Drugs 0.000 title abstract description 11
- MYSWGUAQZAJSOK-UHFFFAOYSA-N ciprofloxacin Chemical compound C12=CC(N3CCNCC3)=C(F)C=C2C(=O)C(C(=O)O)=CN1C1CC1 MYSWGUAQZAJSOK-UHFFFAOYSA-N 0.000 claims abstract description 34
- GSDSWSVVBLHKDQ-JTQLQIEISA-N Levofloxacin Chemical compound C([C@@H](N1C2=C(C(C(C(O)=O)=C1)=O)C=C1F)C)OC2=C1N1CCN(C)CC1 GSDSWSVVBLHKDQ-JTQLQIEISA-N 0.000 claims abstract description 20
- 229960003376 levofloxacin Drugs 0.000 claims abstract description 20
- 229960003405 ciprofloxacin Drugs 0.000 claims abstract description 17
- 101150070420 gyrA gene Proteins 0.000 claims description 49
- 238000001514 detection method Methods 0.000 claims description 42
- 101100139872 Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145) ramR gene Proteins 0.000 claims description 24
- 101150013736 gyrB gene Proteins 0.000 claims description 11
- 101150012629 parE gene Proteins 0.000 claims description 11
- 101100523287 Escherichia coli qnrB4 gene Proteins 0.000 claims description 4
- 239000003153 chemical reaction reagent Substances 0.000 claims description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 208000015181 infectious disease Diseases 0.000 abstract 1
- 206010059866 Drug resistance Diseases 0.000 description 30
- 230000003115 biocidal effect Effects 0.000 description 13
- 230000035772 mutation Effects 0.000 description 11
- 108010041052 DNA Topoisomerase IV Proteins 0.000 description 10
- 206010064571 Gene mutation Diseases 0.000 description 9
- 238000000034 method Methods 0.000 description 8
- 125000001559 cyclopropyl group Chemical group [H]C1([H])C([H])([H])C1([H])* 0.000 description 4
- 229940124350 antibacterial drug Drugs 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 238000012165 high-throughput sequencing Methods 0.000 description 3
- 108020004707 nucleic acids Proteins 0.000 description 3
- 150000007523 nucleic acids Chemical class 0.000 description 3
- 102000039446 nucleic acids Human genes 0.000 description 3
- LISFMEBWQUVKPJ-UHFFFAOYSA-N quinolin-2-ol Chemical compound C1=CC=C2NC(=O)C=CC2=C1 LISFMEBWQUVKPJ-UHFFFAOYSA-N 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- 238000012070 whole genome sequencing analysis Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 239000000427 antigen Substances 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 238000003908 quality control method Methods 0.000 description 2
- 239000002096 quantum dot Substances 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 241000208340 Araliaceae Species 0.000 description 1
- LVZWSLJZHVFIQJ-UHFFFAOYSA-N Cyclopropane Chemical compound C1CC1 LVZWSLJZHVFIQJ-UHFFFAOYSA-N 0.000 description 1
- 238000007400 DNA extraction Methods 0.000 description 1
- 206010061259 Klebsiella infection Diseases 0.000 description 1
- 201000009906 Meningitis Diseases 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 206010036790 Productive cough Diseases 0.000 description 1
- 206010057190 Respiratory tract infections Diseases 0.000 description 1
- 206010040047 Sepsis Diseases 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 238000013480 data collection Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 206010014665 endocarditis Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000001404 mediated effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 208000013223 septicemia Diseases 0.000 description 1
- 210000003802 sputum Anatomy 0.000 description 1
- 208000024794 sputum Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 208000019206 urinary tract infection Diseases 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/106—Pharmacogenomics, i.e. genetic variability in individual responses to drugs and drug metabolism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/22—Klebsiella
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- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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CN202211147291.8A CN115418406B (zh) | 2022-09-19 | 2022-09-19 | 用于预测肺炎克雷伯菌对抗生素药敏表型的特征基因组合、试剂盒及测序方法 |
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CN202211147291.8A CN115418406B (zh) | 2022-09-19 | 2022-09-19 | 用于预测肺炎克雷伯菌对抗生素药敏表型的特征基因组合、试剂盒及测序方法 |
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Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107475421A (zh) * | 2017-09-08 | 2017-12-15 | 中国人民解放军总医院 | 肺炎致病菌耐药基因快速识别基因芯片 |
CN114990241A (zh) * | 2022-06-07 | 2022-09-02 | 天津金匙医学科技有限公司 | 用于预测鲍曼不动杆菌对抗生素药敏表型的特征基因组合、试剂盒及测序方法 |
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2022
- 2022-09-19 CN CN202211147291.8A patent/CN115418406B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN107475421A (zh) * | 2017-09-08 | 2017-12-15 | 中国人民解放军总医院 | 肺炎致病菌耐药基因快速识别基因芯片 |
CN114990241A (zh) * | 2022-06-07 | 2022-09-02 | 天津金匙医学科技有限公司 | 用于预测鲍曼不动杆菌对抗生素药敏表型的特征基因组合、试剂盒及测序方法 |
Non-Patent Citations (5)
Title |
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Alterations in the GyrA subunit of DNA gyrase and the ParC subunit of topoisomerase IV in quinolone-resistant clinical isolates of Klebseilla pneumoniae;Takashi Deguchi等;Antimicrobial Agents and Chemotherapy;第41卷(第3期);第699-701页 * |
Coexistence of qnrB4 and qnrS1 in a clinical strain of klebsiella pneumoniae;Fu-pin Hu等;Acta Pharmacol Sin;第29卷(第3期);第320-324页 * |
Occurrence of qnrB1 and qnrB12 genes, mutation in gyrA and ramR, and expression of efflux pumps in isolates of klebsiella pneumoniae carriers of blaKPC-2;Alexsandra Maria Lima Scavuzzi等;Journal of medical microbiology;第66卷;第477-484页 * |
Plasmid-mediated quinolone resistance;George A. Jacoby等;Microbiol Spectr.;第2卷(第2期);全文 * |
Prediction of fluoroquinolone susceptibility directly from whole-genome sequence data by using liquid chromatography-tandem mass spectrometry to identify mutant genotypes;Wan Ahmad Kamil Wan Nur Ismah等;Antimicrobial Agents and Chemotherapy;第62卷(第3期);摘要、第5页、表1、表2 * |
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Address after: 100071 Room 110, 1st Floor, Building 3, No. 2, Dongbinhe Road, You'anmenwai, Fengtai District, Beijing Patentee after: Beijing Jinshao Medical Laboratory Co.,Ltd. Country or region after: China Patentee after: Tianjin JinKe Medical Technology Co.,Ltd. Patentee after: Tianjin Huazhinuo Technology Co.,Ltd. Address before: 100071 Room 110, 1st Floor, Building 3, No. 2, Dongbinhe Road, You'anmenwai, Fengtai District, Beijing Patentee before: Beijing Jinshao Medical Laboratory Co.,Ltd. Country or region before: China Patentee before: Tianjin JinKe Medical Technology Co.,Ltd. Patentee before: Jinshi Zhizao (Tianjin) Medical Technology Co.,Ltd. |