CN115414419B - Composition, preparation method thereof and application thereof in preparation of products with anti-alcohol effect - Google Patents
Composition, preparation method thereof and application thereof in preparation of products with anti-alcohol effect Download PDFInfo
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- 238000002360 preparation method Methods 0.000 title claims abstract description 33
- 230000000694 effects Effects 0.000 title claims abstract description 24
- 230000002075 anti-alcohol Effects 0.000 title claims abstract description 10
- 244000046146 Pueraria lobata Species 0.000 claims abstract description 93
- 235000010575 Pueraria lobata Nutrition 0.000 claims abstract description 93
- 235000011347 Moringa oleifera Nutrition 0.000 claims abstract description 70
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- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 claims abstract description 61
- 235000008434 ginseng Nutrition 0.000 claims abstract description 61
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 55
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- 241001092040 Crataegus Species 0.000 claims description 52
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 42
- 239000000243 solution Substances 0.000 claims description 39
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- 235000013399 edible fruits Nutrition 0.000 claims 1
- 244000179886 Moringa oleifera Species 0.000 abstract description 64
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/25—Araliaceae (Ginseng family), e.g. ivy, aralia, schefflera or tetrapanax
- A61K36/258—Panax (ginseng)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/488—Pueraria (kudzu)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/734—Crataegus (hawthorn)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P39/00—General protective or antinoxious agents
- A61P39/02—Antidotes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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Abstract
The invention discloses a composition, a preparation method thereof and application thereof in preparing products with anti-alcohol effect. The composition comprises the following raw material components in parts by weight: 20-40 parts of moringa seed, 8-20 parts of kudzuvine root, 15-30 parts of kudzuvine flower, 15-30 parts of hawthorn and 3-10 parts of ginseng. The composition has the advantages of accelerating alcohol metabolism and better anti-alcohol effect by compounding the kudzuvine root, the kudzuvine flower, the hawthorn and the ginseng with the moringa seed; in addition, moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng also have the effects of nourishing and protecting liver, and have the effect of promoting the repair and regeneration of liver cells; the composition can be used as active ingredient for preparing product with anti-hangover effect, and can increase alcohol content and prevent drunk after drinking, and reduce discomfort after drinking and reduce liver cell invasion.
Description
Technical Field
The invention relates to the technical field of natural products, in particular to a composition, a preparation method thereof and application thereof in preparing products with an anti-alcohol effect.
Background
Since ancient times, chinese wine culture is popular, and proper drinking can eliminate fatigue, relieve nervous tension and ease channels and collaterals. However, excessive drinking has a great influence on human health, and clinical symptoms such as dizziness, vomiting, thirst and the like appear in a light state; severe damage to gastrointestinal tract and liver tissue may result in alcoholic fatty liver, liver cirrhosis, liver cancer, etc. The international cancer institute, subordinate to the world health organization, has already clearly listed alcohols as a class of carcinogens with a clear carcinogenicity to humans. In 2012, alcohol consumption caused about 330 thousands of deaths, accounting for 5.9% of the total worldwide deaths.
Therefore, selecting proper food, health care products or medicines to achieve sobering up, dispelling alcohol and reducing damage of alcohol to gastrointestinal tract and liver is important for people in China. At present, the products for dispelling effects of alcohol at home and abroad can be roughly divided into four types: western medicines mainly comprising diuretics and chemical synthesis medicines, traditional Chinese medicines for protecting liver and nourishing stomach, protein peptide health products and health products mainly comprising enzyme activities related to alcohol metabolism by improving.
Moringa oleifera, ganoderma lucidum, american ginseng and American ginseng are called as "three treasures in the world". Moringa oleifera is also called drumstick tree, family Morindae of the order Pelargoniaceae, and is a perennial tropical arbor native to India. Besides a small amount of moringa seeds used for planting, a large amount of moringa seeds are used as production raw materials, and have good application values in the fields of foods, medicines, health care, cosmetics and the like, such as liquid purification, extraction and preparation of active substances of high-grade cosmetics such as water retention, sun protection and the like. The moringa seed is the seed of moringa tree, and has good application value in the fields of food, medicine, health care, cosmetics and the like.
Although the prior art reports that moringa seeds have the effects of dispelling effects of alcohol and protecting liver; however, in the practical application process, the effect of the moringa seed alone is not very remarkable. Therefore, the composition capable of accelerating alcohol metabolism and improving alcohol effect dispelling capability has important application value.
Disclosure of Invention
In order to overcome at least one technical problem existing in the prior art, the invention provides a composition and a preparation method thereof. Research shows that the composition can accelerate alcohol metabolism and has good anti-alcohol effect.
The technical problems to be solved by the invention are realized by the following technical scheme:
a composition comprising the following raw material components: moringa seed, radix Puerariae, flos Puerariae Lobatae, fructus crataegi, and Ginseng radix.
Preferably, the composition comprises the following raw material components in parts by weight: 20-40 parts of moringa seed, 8-20 parts of kudzuvine root, 15-30 parts of kudzuvine flower, 15-30 parts of hawthorn and 3-10 parts of ginseng.
The inventor discovers in the research that the kudzuvine root, the kudzuvine flower, the hawthorn, the ginseng and the moringa seed are compounded to accelerate alcohol metabolism, thereby playing a role in dispelling the effects of alcohol.
Further preferably, the composition comprises the following raw material components in parts by weight: 20-30 parts of moringa seed, 10-15 parts of kudzuvine root, 20-25 parts of kudzuvine flower, 20-25 parts of hawthorn and 5-8 parts of ginseng.
Most preferably, the composition comprises the following raw material components in parts by weight: 22 parts of moringa seed, 11 parts of kudzuvine root, 22 parts of kudzuvine flower, 22 parts of hawthorn and 6 parts of ginseng.
The invention also provides a preparation method of the composition, which comprises the following steps:
mixing Moringa seed, radix Puerariae, flos Puerariae Lobatae, fructus crataegi and Ginseng radix, extracting with organic solvent, concentrating and drying the extractive solution to obtain organic solvent extract.
Preferably, the extraction is heating reflux extraction, and the extraction time is 1-3 h.
Preferably, the heating means heating to a boiling state.
Preferably, the organic solvent is a mixed organic solvent consisting of n-butanol and acetone; wherein the volume ratio of the n-butanol to the acetone is 2-4:1.
Most preferably, the volume ratio of n-butanol to acetone is 3:1.
The inventor finds that the difference of extraction solvents of moringa seeds, kudzuvine roots, kudzuvine flowers, hawthorns and ginseng are taken as raw materials in research, and the difference plays an important role in the speed of alcohol metabolism of the prepared extract. The inventors have surprisingly found in a number of experiments that: the extract is prepared from moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng by extracting with a mixed organic solvent consisting of n-butanol and acetone, and has a higher alcohol metabolism rate than the extract obtained by extracting with conventional ethanol, and also has a higher alcohol metabolism rate than the extract obtained by extracting with other organic solvents or a combination of organic solvents.
Preferably, the dosage ratio of the total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng to the organic solvent is 1 g:10-20 mL.
Most preferably, the ratio of the total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng to the organic solvent is 1g to 15mL.
Further, the organic solvent extract is put on a macroporous resin column, and eluting impurities by using an ethanol aqueous solution with the volume fraction of 12-15% and the volume of 3-5 times of the column volume; then eluting with ethanol water solution with the volume fraction of 26-28% and the volume fraction of 4-6 times of the column volume, collecting the eluent eluted from the ethanol water solution with the volume fraction of 26-28%, concentrating and drying to obtain the composition.
Most preferably, the organic solvent extract is loaded on a macroporous resin column, and eluting with 4 times of ethanol water solution with volume fraction of 13% to remove impurities; then eluting with ethanol water solution with volume fraction of 27% and 5 times of column volume, collecting eluate eluted with ethanol water solution with volume fraction of 27%, concentrating, and drying to obtain the composition.
Most preferably, the macroporous resin column is an HP-20 macroporous resin column.
In order to further increase the metabolism rate of the composition for alcohol, the inventor further adopts a macroporous resin column to enrich active ingredients capable of accelerating alcohol metabolism. The inventor researches find that under different macroporous resin elution conditions, the effective components obtained by enrichment have great difference on alcohol metabolism speed; the inventor has shown through a great deal of experimental study that: on the basis of taking moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng as raw materials and adopting an extract obtained by extracting a mixed organic solvent consisting of n-butyl alcohol and acetone, the composition prepared by adopting any macroporous resin elution condition can not be used for further improving or greatly improving the metabolism speed of alcohol; only the composition prepared under the condition of the macroporous resin elution can further and greatly improve the metabolism speed of alcohol, but the composition prepared under the condition of other macroporous resin elution can not further or greatly improve the metabolism speed of alcohol.
The invention also provides application of the composition in preparation of products with anti-alcohol effect.
Preferably, the anti-alcohol effect is achieved by increasing the metabolic rate of alcohol.
Preferably, the product is a drug, a food or a health product.
The beneficial effects are that: the invention provides a composition with novel composition, which has the advantages that after the kudzuvine root, the kudzuvine flower, the hawthorn, the ginseng and the moringa seed are compounded, the alcohol metabolism can be quickened, and the composition has a better alcohol dispelling effect; the composition prepared by the method has high metabolism speed on alcohol and excellent anti-alcohol effect.
In addition, moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng also have the effects of nourishing and protecting liver, and have the effect of promoting the repair and regeneration of liver cells; the composition can be used as active ingredient for preparing product with anti-hangover effect, and can increase alcohol content and prevent drunk after drinking, and reduce discomfort after drinking and reduce liver cell invasion.
Detailed Description
The present invention is further explained below with reference to specific examples, which are not intended to limit the present invention in any way.
Example 1 preparation of the composition
The raw materials comprise the following components in parts by weight: 22 parts of moringa seed, 11 parts of kudzuvine root, 22 parts of kudzuvine flower, 22 parts of hawthorn and 6 parts of ginseng;
the preparation method comprises the following steps: mixing moringa seed, radix puerariae, flos puerariae lobatae, hawthorns and ginseng, adding an organic solvent, heating to a boiling state, carrying out heating reflux extraction for 2 hours, concentrating and drying an extracting solution after the extraction is finished to obtain an organic solvent extract, and obtaining the composition;
wherein the dosage ratio of the total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng to the organic solvent is 1g to 15mL; the organic solvent is ethanol with the volume fraction of 95%.
Example 2 preparation of the composition
The raw materials comprise the following components in parts by weight: 22 parts of moringa seed, 11 parts of kudzuvine root, 22 parts of kudzuvine flower, 22 parts of hawthorn and 6 parts of ginseng;
the preparation method comprises the following steps: mixing moringa seed, radix puerariae, flos puerariae lobatae, hawthorns and ginseng, adding an organic solvent, heating to a boiling state, carrying out heating reflux extraction for 2 hours, concentrating and drying an extracting solution after the extraction is finished to obtain an organic solvent extract, and obtaining the composition;
wherein the dosage ratio of the total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng to the organic solvent is 1g to 15mL; the organic solvent consists of n-butanol and acetone in a volume ratio of 3:1.
Example 3 preparation of the composition
The raw materials comprise the following components in parts by weight: 20 parts of moringa seed, 20 parts of kudzuvine root, 15 parts of kudzuvine flower, 15 parts of hawthorn and 3 parts of ginseng;
the preparation method comprises the following steps: mixing moringa seed, radix puerariae, flos puerariae lobatae, hawthorns and ginseng, adding an organic solvent, heating to a boiling state, carrying out heating reflux extraction for 1h, concentrating and drying an extracting solution after extraction is finished to obtain an organic solvent extract, and obtaining the composition;
wherein the dosage ratio of the total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng to the organic solvent is 1g to 20mL; the organic solvent consists of n-butanol and acetone in a volume ratio of 4:1.
Example 4 preparation of the composition
The raw materials comprise the following components in parts by weight: 40 parts of moringa seed, 8 parts of kudzuvine root, 30 parts of kudzuvine flower, 30 parts of hawthorn and 10 parts of ginseng;
the preparation method comprises the following steps: mixing moringa seed, radix puerariae, flos puerariae lobatae, hawthorns and ginseng, adding an organic solvent, heating to a boiling state, carrying out heating reflux extraction for 3 hours, concentrating and drying an extracting solution after extraction is finished to obtain an organic solvent extract, and obtaining the composition;
wherein the dosage ratio of the total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng to the organic solvent is 1g to 10mL; the organic solvent consists of n-butanol and acetone in a volume ratio of 2:1.
Example 5 preparation of the composition
The raw materials comprise the following components in parts by weight: 22 parts of moringa seed, 11 parts of kudzuvine root, 22 parts of kudzuvine flower, 22 parts of hawthorn and 6 parts of ginseng;
the preparation method comprises the following steps:
(1) Mixing Moringa seed, radix Puerariae, flos Puerariae Lobatae, fructus crataegi and Ginseng radix, adding organic solvent, heating to boiling state, reflux-extracting for 2 hr, concentrating and drying the extractive solution to obtain organic solvent extract; wherein the dosage ratio of the total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng to the organic solvent is 1g to 15mL; the organic solvent consists of n-butanol and acetone in a volume ratio of 3:1;
(2) Loading the organic solvent extract onto macroporous resin column (macroporous resin column is filled with macroporous resin of HP-20, the weight of macroporous resin of HP-20 is 40 times of that of the organic solvent extract), eluting with ethanol water solution with volume fraction of 13% and 4 times of column volume to remove impurities; then eluting with ethanol water solution with volume fraction of 27% and 5 times of column volume, collecting eluate eluted with ethanol water solution with volume fraction of 27%, concentrating, and drying to obtain the composition.
Example 6 preparation of the composition
The raw materials comprise the following components in parts by weight: 22 parts of moringa seed, 11 parts of kudzuvine root, 22 parts of kudzuvine flower, 22 parts of hawthorn and 6 parts of ginseng;
the preparation method comprises the following steps: mixing moringa seed, radix puerariae, flos puerariae lobatae, hawthorns and ginseng, adding an organic solvent, heating to a boiling state, carrying out heating reflux extraction for 2 hours, concentrating and drying an extracting solution after the extraction is finished to obtain an organic solvent extract, and obtaining the composition;
wherein the dosage ratio of the total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng to the organic solvent is 1g to 15mL; the organic solvent is acetone.
Example 7 preparation of the composition
The raw materials comprise the following components in parts by weight: 22 parts of moringa seed, 11 parts of kudzuvine root, 22 parts of kudzuvine flower, 22 parts of hawthorn and 6 parts of ginseng;
the preparation method comprises the following steps: mixing moringa seed, radix puerariae, flos puerariae lobatae, hawthorns and ginseng, adding an organic solvent, heating to a boiling state, carrying out heating reflux extraction for 2 hours, concentrating and drying an extracting solution after the extraction is finished to obtain an organic solvent extract, and obtaining the composition;
wherein the dosage ratio of the total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng to the organic solvent is 1g to 15mL; the organic solvent is n-butanol.
Example 8 preparation of the composition
The raw materials comprise the following components in parts by weight: 22 parts of moringa seed, 11 parts of kudzuvine root, 22 parts of kudzuvine flower, 22 parts of hawthorn and 6 parts of ginseng;
the preparation method comprises the following steps: mixing moringa seed, radix puerariae, flos puerariae lobatae, hawthorns and ginseng, adding an organic solvent, heating to a boiling state, carrying out heating reflux extraction for 2 hours, concentrating and drying an extracting solution after the extraction is finished to obtain an organic solvent extract, and obtaining the composition;
wherein the dosage ratio of the total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng to the organic solvent is 1g to 15mL; the organic solvent is methanol with volume fraction.
Example 9 preparation of the composition
The raw materials comprise the following components in parts by weight: 22 parts of moringa seed, 11 parts of kudzuvine root, 22 parts of kudzuvine flower, 22 parts of hawthorn and 6 parts of ginseng;
the preparation method comprises the following steps: mixing moringa seed, radix puerariae, flos puerariae lobatae, hawthorns and ginseng, adding an organic solvent, heating to a boiling state, carrying out heating reflux extraction for 2 hours, concentrating and drying an extracting solution after the extraction is finished to obtain an organic solvent extract, and obtaining the composition;
wherein the dosage ratio of the total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng to the organic solvent is 1g to 15mL; the organic solvent consists of n-butanol and methanol in a volume ratio of 3:1.
Preparation of the composition of comparative example 1
The raw materials comprise the following components in parts by weight: 22 parts of moringa seed, 11 parts of kudzuvine root, 22 parts of kudzuvine flower, 22 parts of hawthorn and 6 parts of ginseng;
the preparation method comprises the following steps:
(1) Mixing Moringa seed, radix Puerariae, flos Puerariae Lobatae, fructus crataegi and Ginseng radix, adding organic solvent, heating to boiling state, reflux-extracting for 2 hr, concentrating and drying the extractive solution to obtain organic solvent extract; wherein the dosage ratio of the total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng to the organic solvent is 1g to 15mL; the organic solvent consists of n-butanol and acetone in a volume ratio of 3:1;
(2) Loading the organic solvent extract onto macroporous resin column (macroporous resin column is filled with macroporous resin of HP-20, the weight of macroporous resin of HP-20 is 40 times of that of the organic solvent extract), eluting with ethanol water solution with volume fraction of 5% and 4 times of column volume to remove impurities; then eluting with ethanol water solution with volume fraction of 15% and 5 times of column volume, collecting eluate eluted with ethanol water solution with volume fraction of 15%, concentrating, and drying to obtain the composition.
Comparative example 1 differs from example 5 in that the elution conditions of the macroporous resin column are different, and the elution conditions of comparative example 1 are: eluting with ethanol water solution with volume fraction of 5% to remove impurities; then eluting with 15% ethanol water solution; whereas the elution conditions for example 5 were: eluting with 13% ethanol water solution to remove impurities; then eluting with 27% ethanol water solution.
Preparation of comparative example 2 composition
The raw materials comprise the following components in parts by weight: 22 parts of moringa seed, 11 parts of kudzuvine root, 22 parts of kudzuvine flower, 22 parts of hawthorn and 6 parts of ginseng;
the preparation method comprises the following steps:
(1) Mixing Moringa seed, radix Puerariae, flos Puerariae Lobatae, fructus crataegi and Ginseng radix, adding organic solvent, heating to boiling state, reflux-extracting for 2 hr, concentrating and drying the extractive solution to obtain organic solvent extract; wherein the dosage ratio of the total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng to the organic solvent is 1g to 15mL; the organic solvent consists of n-butanol and acetone in a volume ratio of 3:1;
(2) Loading the organic solvent extract onto macroporous resin column (macroporous resin column is filled with macroporous resin of HP-20, the weight of macroporous resin of HP-20 is 40 times of that of the organic solvent extract), eluting with ethanol water solution with volume fraction of 20% and 4 times of column volume to remove impurities; then eluting with ethanol water solution with volume fraction of 40% and 5 times of column volume, collecting eluate eluted with ethanol water solution with volume fraction of 40%, concentrating, and drying to obtain the composition.
Comparative example 2 differs from example 5 in that the elution conditions of the macroporous resin column are different, and the elution conditions of comparative example 2 are: eluting with 20% ethanol water solution to remove impurities; then eluting with 40% ethanol water solution; whereas the elution conditions for example 5 were: eluting with 13% ethanol water solution to remove impurities; then eluting with 27% ethanol water solution.
Experimental example 1
Taking male SD rats with the weight of 300 g+/-30 g, and dividing the male SD rats into 12 groups of 10 rats; wherein 11 groups are experimental groups, and 1 group is a model group; the experimental group irrigates the stomach with the compositions prepared in examples 1 to 9 of the invention or the compositions prepared in comparative examples 1 to 2 according to the dosage of 10mg/kg 1h before the gastric lavage of alcohol; filling the model group with distilled water with the same amount; after the administration of the lavage, the experimental group and the model group are filled with 38% alcohol according to the volume fraction of 1 ml/kg. 2 hours after stomach alcohol infusion, testing the alcohol content in the blood of the rat; the test results are shown in Table 1;
wherein, experimental groups 1 to 9 were respectively perfused with the compositions prepared in examples 1 to 9; the compositions prepared in comparative examples 1 to 2 were respectively intragastric in experimental groups 10 to 11.
Table 1.
| Experimental group | Test compositions | Blood alcohol concentration (mg/100 mL) |
| Model group | - | 51.9±6.3 |
| Experiment group 1 | Example 1 composition | 44.1±4.3 |
| Experiment group 2 | Example 2 composition | 30.2±2.9 |
| Experiment group 3 | Example 3 composition | 32.7±3.4 |
| Experiment group 4 | Example 4 composition | 31.3±3.1 |
| Experiment group 5 | Example 5 composition | 8.1±1.7 |
| Experiment group 6 | Example 6 composition | 42.3±3.9 |
| Experiment group 7 | Example 7 composition | 40.8±4.1 |
| Experiment group 8 | Example 8 composition | 45.2±4.4 |
| Experiment group 9 | Example 9 composition | 41.1±3.6 |
| Experiment group 10 | Comparative example 1 composition | 28.3±2.5 |
| Experiment group 11 | Comparative example 2 composition | 26.7±2.1 |
As can be seen from the experimental data in table 1, the blood alcohol concentration in rats of experimental group 1 is significantly lower than that of the model group, which indicates that: the composition prepared by compounding the kudzuvine root, the kudzuvine flower, the hawthorn and the ginseng with the moringa seed can accelerate alcohol metabolism and has the effect of dispelling alcohol effects.
From the experimental data in table 1, it can be seen that, compared with the model group, the blood alcohol concentration in the rats in the experimental groups 2 to 4 is substantially smaller than that in the model group, and is also substantially smaller than that in the experimental group 1; in addition, the blood alcohol concentration in rats of experimental groups 6 to 9 is equivalent to that of experimental group 1; the blood alcohol concentration in rats of experimental groups 2-4 is also substantially less than that of experimental groups 6-9. This illustrates: the moringa seed, the kudzuvine root, the kudzuvine flower, the hawthorn and the ginseng are taken as raw materials, and different extraction solvents play an important role in the speed of alcohol metabolism of the prepared extract. In the invention, moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng are taken as raw materials, and the extract obtained by extracting the moringa seed, the kudzuvine flower, the hawthorn and the ginseng by adopting a mixed organic solvent consisting of n-butyl alcohol and acetone has a higher alcohol metabolism speed than that of the extract obtained by adopting the conventional ethanol extraction, and is also higher than that of the extract obtained by adopting other organic solvents or a combination of the organic solvents.
As can be seen from the experimental data in table 1, the blood alcohol concentration in rats of experimental group 5 is further substantially less than that of experimental group 2; this illustrates: the composition prepared under the macroporous resin elution condition can further greatly improve the metabolism speed of alcohol on the basis of an extract obtained by extracting moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng serving as raw materials by adopting a mixed organic solvent consisting of n-butyl alcohol and acetone.
As can be seen from the experimental data in table 1, the blood alcohol concentration in rats of experimental groups 10 to 11 was not significantly reduced compared with that of experimental group 2; this shows that the macroporous resin elution conditions of the invention are very critical, and the effective components obtained by enrichment have great difference on alcohol metabolism speed under different macroporous resin elution conditions; on the basis of taking moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng as raw materials and adopting an extract obtained by extracting a mixed organic solvent consisting of n-butyl alcohol and acetone, the composition prepared by adopting any macroporous resin elution condition can not be used for further improving or greatly improving the metabolism speed of alcohol; only under the condition of the macroporous resin elution (namely eluting with 12-15% ethanol water solution by volume fraction and then eluting with 26-28% ethanol water solution) can the composition prepared by the invention further greatly improve the metabolism rate of alcohol, but the composition prepared under other macroporous resin elution conditions can not further greatly improve the metabolism rate of alcohol.
Claims (6)
1. The composition with the anti-alcohol effect is characterized by being prepared from the following raw material components in parts by weight: 20-40 parts of moringa seed, 8-20 parts of kudzuvine root, 15-30 parts of kudzuvine flower, 15-30 parts of hawthorn and 3-10 parts of ginseng;
a method of preparing the composition comprising the steps of:
mixing Moringa seed, radix Puerariae, flos Puerariae Lobatae, fructus crataegi and Ginseng radix, adding organic solvent, extracting, concentrating and drying the extractive solution to obtain organic solvent extract;
loading the organic solvent extract on a macroporous resin column, eluting with an ethanol aqueous solution with the volume fraction of 12-15% and the volume of 3-5 times of the column volume to remove impurities; then eluting with an ethanol water solution with the volume fraction of 26-28% and 4-6 times of the column volume, collecting an eluent eluted from the ethanol water solution with the volume fraction of 26-28%, concentrating and drying to obtain the composition;
the extraction is heating reflux extraction, and the extraction time is 1-3 hours;
the organic solvent is a mixed organic solvent consisting of n-butanol and acetone; wherein the volume ratio of n-butanol to acetone is 2-4:1;
the dosage ratio of the total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn and ginseng to the organic solvent is 1 g:10-20 mL.
2. The composition according to claim 1, which is prepared from the following raw material components in parts by weight: 20-30 parts of moringa seed, 10-15 parts of kudzuvine root, 20-25 parts of kudzuvine flower, 20-25 parts of hawthorn and 5-8 parts of ginseng.
3. The composition according to claim 1, which is prepared from the following raw material components in parts by weight: 22 parts of moringa seed, 11 parts of kudzuvine root, 22 parts of kudzuvine flower, 22 parts of hawthorn and 6 parts of ginseng.
4. The composition of claim 1, wherein the volume ratio of n-butanol to acetone is 3:1.
5. The composition of claim 1, wherein the ratio of total weight of moringa seed, kudzuvine root, kudzuvine flower, hawthorn fruit and ginseng to organic solvent is 1g to 15ml.
6. Use of a composition according to any one of claims 1 to 5 for the preparation of a product having anti-hangover effect, characterized in that said product is a pharmaceutical.
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| CN107927260A (en) * | 2017-12-06 | 2018-04-20 | 张振亮 | Wild Pueraria/Pueravia flower tea, preparation method and application |
| CN109232702A (en) * | 2018-10-11 | 2019-01-18 | 云南三七科技有限公司 | A kind of preparation method of ginseng saponin Rh 2 |
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| CN1197672A (en) * | 1998-03-27 | 1998-11-04 | 马宏 | Drunkenness dispelling liquid |
| CN102085206A (en) * | 2009-12-07 | 2011-06-08 | 北京本草天源药物研究院 | Medicinal composition |
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| CN109232702A (en) * | 2018-10-11 | 2019-01-18 | 云南三七科技有限公司 | A kind of preparation method of ginseng saponin Rh 2 |
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