CN115413666B - 一种猕猴桃授粉液及制备方法和猕猴桃授粉方法 - Google Patents
一种猕猴桃授粉液及制备方法和猕猴桃授粉方法 Download PDFInfo
- Publication number
- CN115413666B CN115413666B CN202211170275.0A CN202211170275A CN115413666B CN 115413666 B CN115413666 B CN 115413666B CN 202211170275 A CN202211170275 A CN 202211170275A CN 115413666 B CN115413666 B CN 115413666B
- Authority
- CN
- China
- Prior art keywords
- kiwi fruit
- pollination
- kiwi
- brassinosteroids
- liquid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 244000298697 Actinidia deliciosa Species 0.000 title claims abstract description 61
- 235000009436 Actinidia deliciosa Nutrition 0.000 title claims abstract description 59
- 230000010152 pollination Effects 0.000 title claims abstract description 57
- 239000007788 liquid Substances 0.000 title claims abstract description 28
- 238000000034 method Methods 0.000 title claims abstract description 15
- 238000002360 preparation method Methods 0.000 title abstract description 8
- 150000001647 brassinosteroids Chemical class 0.000 claims abstract description 21
- 241000196324 Embryophyta Species 0.000 claims abstract description 20
- 241000958510 Stropharia rugosoannulata Species 0.000 claims abstract description 20
- 150000004676 glycans Chemical class 0.000 claims abstract description 20
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 20
- 239000005017 polysaccharide Substances 0.000 claims abstract description 20
- 235000021552 granulated sugar Nutrition 0.000 claims abstract description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 19
- 239000002994 raw material Substances 0.000 claims abstract description 14
- 229920002752 Konjac Polymers 0.000 claims description 15
- 239000000252 konjac Substances 0.000 claims description 11
- 239000000725 suspension Substances 0.000 claims description 10
- SRLOHQKOADWDBV-NRONOFSHSA-M sodium;[(2r)-2,3-di(octadecanoyloxy)propyl] 2-(2-methoxyethoxycarbonylamino)ethyl phosphate Chemical compound [Na+].CCCCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCCNC(=O)OCCOC)OC(=O)CCCCCCCCCCCCCCCCC SRLOHQKOADWDBV-NRONOFSHSA-M 0.000 claims description 9
- 235000019823 konjac gum Nutrition 0.000 claims description 8
- 235000010485 konjac Nutrition 0.000 claims description 7
- 239000000463 material Substances 0.000 claims description 7
- 238000001816 cooling Methods 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 238000005507 spraying Methods 0.000 claims description 5
- 238000001132 ultrasonic dispersion Methods 0.000 claims description 5
- 238000005303 weighing Methods 0.000 claims description 3
- 235000013399 edible fruits Nutrition 0.000 abstract description 9
- 235000009434 Actinidia chinensis Nutrition 0.000 abstract description 5
- 239000003292 glue Substances 0.000 abstract description 2
- 230000000052 comparative effect Effects 0.000 description 4
- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 description 3
- 244000247812 Amorphophallus rivieri Species 0.000 description 3
- 235000001206 Amorphophallus rivieri Nutrition 0.000 description 3
- 229920002581 Glucomannan Polymers 0.000 description 3
- 229940046240 glucomannan Drugs 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000002195 synergetic effect Effects 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000010154 cross-pollination Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/22—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom rings with more than six members
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/24—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients to enhance the sticking of the active ingredients
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Pest Control & Pesticides (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Botany (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Toxicology (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
本发明涉及猕猴桃种植领域，具体涉及一种猕猴桃授粉液及制备方法和猕猴桃授粉方法，该一种猕猴桃授粉液由以下质量百分比的原料制备所得：芸苔素甾醇0.05～0.07%；DSPE‑MPEG2000 1.5～3%；大球盖菇多糖1～3%；白砂糖0.3～0.7%，植物源胶30%，其余为水。本发明通过猕猴桃授粉配方的优化设计，使得花粉无论在阴雨天还是正午午后(雌花花柱粘液稀少时)都可以稳定的吸附在雌花柱头上，授粉质量稳定，协同芸苔素甾醇和大球盖菇多糖的添加，可以显著提高猕猴桃的座果率和优果率。
Description
技术领域
本发明涉及猕猴桃种植领域，具体涉及一种猕猴桃授粉液及制备方法和猕猴桃授粉方法。
背景技术
猕猴桃为雌雄异株授粉植物，人工辅助授粉的质量是决定最终产品数量及品质的关键环节。目前，国内猕猴桃人工授粉主要以干粉授粉为主，其用时长，受限制条件多，如正午午后柱头干燥或阴雨天等均会影响其授粉的质量。急需提供一种授粉质量稳定且限制条件少的猕猴桃人工授粉技术。
发明内容
为解决上述问题，本发明提供了一种猕猴桃授粉液及制备方法和猕猴桃授粉方法，在正午午后柱头干燥后或阴雨天均可进行授粉，授粉质量稳定。
为实现上述目的，本发明采取的技术方案为：
一种猕猴桃授粉液，由以下质量百分比的原料制备所得：
芸苔素甾醇 0.05～0.07%；DSPE-MPEG2000 1.5～3 %；大球盖菇多糖1～3%；白砂糖0.3～0.7%，植物源胶30%，其余为水。
作为优选，由以下质量百分比的原料制备所得：
芸苔素甾醇 0.05%；DSPE-MPEG2000 1.5%；大球盖菇多糖1%；白砂糖 0.3%，植物源胶30%，其余为水。
作为优选，由以下质量百分比的原料制备所得：
芸苔素甾醇0.07%；DSPE-MPEG2000 3 %；大球盖菇多糖3%；白砂糖 0.7%，植物源胶30%，其余为水。
作为优选，由以下质量百分比的原料制备所得：
芸苔素甾醇 0.06%；DSPE-MPEG2000 2.25%；大球盖菇多糖2%；白砂糖 0.5%，植物源胶30%，其余为水。
本发明还提供了一种制备上述的猕猴桃授粉液的方法，其特征在于：包括如下步骤：
S1、按质量百分比称取各组分；
S2、将植物源胶超声分散于水中，形成植物源胶悬混液；
S3、在65～70℃，0.06MP条件下，向植物源胶悬混液中依次加入大球盖菇多糖、芸苔素甾醇、白砂糖、DSPE-MPEG2000，超声分散30min，冷却至常温，密封保存。
本发明还提供了一种猕猴桃授粉方法，采用上述的猕猴桃授粉液与花粉混合后，通过喷雾器向猕猴桃雌株的花朵上进行喷洒授粉，其中，猕猴桃授粉液与花粉的体积质量比为：100:1～2，按每株猕猴桃雌株0.1mL～0.2mL的进行喷洒授粉。
本发明具有以下有益效果：
通过猕猴桃授粉配方的优化设计，使得花粉无论在阴雨天还是正午午后(雌花花柱粘液稀少时)都可以稳定的吸附在雌花柱头上，授粉质量稳定，协同芸苔素甾醇和大球盖菇多糖的添加，可以显著提高猕猴桃的座果率和优果率。
具体实施方式
下面结合具体实施例对本发明进行详细说明。以下实施例将有助于本领域的技术人员进一步理解本发明，但不以任何形式限制本发明。应当指出的是，对本领域的普通技术人员来说，在不脱离本发明构思的前提下，还可以做出若干变形和改进。这些都属于本发明的保护范围。
实施例1
一种猕猴桃授粉液，由以下质量百分比的原料制备所得：
芸苔素甾醇 0.05%；DSPE-MPEG2000 1.5%；大球盖菇多糖1%；白砂糖 0.3%，魔芋胶30%，其余为水。
实施例2
一种猕猴桃授粉液，由以下质量百分比的原料制备所得：
芸苔素甾醇0.07%；DSPE-MPEG2000 3 %；大球盖菇多糖3%；白砂糖 0.7%，魔芋胶30%，其余为水。
实施例3
一种猕猴桃授粉液，由以下质量百分比的原料制备所得：
芸苔素甾醇 0.06%；DSPE-MPEG2000 2.25%；大球盖菇多糖2%；白砂糖 0.5%，魔芋胶30%，其余为水。
制备方法：
S1、按质量百分比称取各组分；
S2、将魔芋胶超声分散于水中，形成植物源胶悬混液；
S3、在65～70℃，0.06MP条件下，向植物源胶悬混液中依次加入大球盖菇多糖、芸苔素甾醇、白砂糖、DSPE-MPEG2000，超声分散30min，冷却至常温，密封保存。
对比例1
一种猕猴桃授粉液，由以下质量百分比的原料制备所得：
芸苔素甾醇 0.05%；大球盖菇多糖1%；白砂糖 0.3%，魔芋胶30%，其余为水。
制备时，将魔芋胶超声分散于水中，形成植物源胶悬混液；在65～70℃，0.06MP条件下，向植物源胶悬混液中依次加入大球盖菇多糖、芸苔素甾醇、白砂糖，超声分散30min，冷却至常温，密封保存。
对比例2
一种猕猴桃授粉液，由以下质量百分比的原料制备所得：
芸苔素甾醇0.07%；DSPE-MPEG2000 3 %；白砂糖 0.7%，魔芋胶30%，其余为水。
制备时，将魔芋胶超声分散于水中，形成植物源胶悬混液；在65～70℃，0.06MP条件下，向植物源胶悬混液中依次加入芸苔素甾醇、白砂糖、DSPE-MPEG2000，超声分散30min，冷却至常温，密封保存。
供试品种为8年生的高接红阳猕猴桃，在猕猴桃果园划定3个试验区A、B、C和3对照区D、E、F，各20株。
试验区A：在1/2～2/3的雌花开放时，于露水干后进行喷雾授粉，大风天不宜进行，将实施例1所得的猕猴桃授粉液与花粉按100:2的比例混合后，通过喷雾器向猕猴桃雌株的花朵上进行喷雾授粉，喷雾器距离花约15cm左右，每朵花喷2下，也就是0.2mL。
试验区B：采用实施例2所得的猕猴桃授粉液，其余设计同试验区A；
试验区C：采用实施例3所得的猕猴桃授粉液，其余设计同试验区A；
对照区D：采用对比例1所得的猕猴桃授粉液，其余设计同试验区A；
对照区E：采用对比例2所得的猕猴桃授粉液，其余设计同试验区A
对照区F：采用人工点粉，用毛笔或棉签对供试花朵进行人工点授。
检测指标：花朵坐果率。
结果：
可见，试验区A、B和C的花朵坐果率均显著高于对照区D、E、F，同时，基于对照区D和对照区E和试验区的对比情况，可以得出DSPE-MPEG2000 、大球盖菇多糖两者同时添加时，具有协同增效的作用。
另，选取两个试验区，各20株，其中一个试验区选择阴雨天，采用试验区A的授粉方式授粉；另一个试验区选择在正午午后(雌花花柱粘液稀少时)采用试验区A的授粉方式授粉，统计两个试验区的花朵坐果率，结果：阴雨天组：81.17%；正午午后组：81.39%，可见，本发明的授粉方法无论在阴雨天还是正午午后(雌花花柱粘液稀少时)都可以使得花粉稳定的吸附在雌花柱头上，授粉质量稳定。
选取各试验区和对照区的15个典型果实（个头最大的），测定其单果质量，结果：试验区所得的猕猴桃单果质量均显著高于对照区E和F，高于对照区D，较对照区F，增重20%左右。
以上对本发明的具体实施例进行了描述。需要理解的是，本发明并不局限于上述特定实施方式，本领域技术人员可以在权利要求的范围内做出各种变化或修改，这并不影响本发明的实质内容。在不冲突的情况下，本申请的实施例和实施例中的特征可以任意相互组合。
Claims (8)
1.一种猕猴桃授粉液，其特征在于：由以下质量百分比的原料制备所得：
芸苔素甾醇 0.05～0.07%；DSPE-MPEG2000 1.5～3 %；大球盖菇多糖1～3%；白砂糖0.3～0.7%，魔芋胶30%，其余为水。
2.如权利要求1所述的一种猕猴桃授粉液，其特征在于：由以下质量百分比的原料制备所得：
芸苔素甾醇 0.05%；DSPE-MPEG2000 1.5%；大球盖菇多糖1%；白砂糖 0.3%，魔芋胶30%，其余为水。
3.如权利要求1所述的一种猕猴桃授粉液，其特征在于：由以下质量百分比的原料制备所得：
芸苔素甾醇0.07%；DSPE-MPEG2000 3 %；大球盖菇多糖3%；白砂糖 0.7%，魔芋胶30%，其余为水。
4.如权利要求1所述的一种猕猴桃授粉液，其特征在于：由以下质量百分比的原料制备所得：
芸苔素甾醇 0.06%；DSPE-MPEG2000 2.25%；大球盖菇多糖2%；白砂糖 0.5%，魔芋胶30%，其余为水。
5.一种制备如权利要求1～4任一项所述的猕猴桃授粉液的方法，其特征在于：包括如下步骤：
S1、按质量百分比称取各组分；
S2、将魔芋胶超声分散于水中，形成魔芋胶悬混液；
S3、在65～70℃，0.06MP条件下，向魔芋胶悬混液中依次加入大球盖菇多糖、芸苔素甾醇、白砂糖、DSPE-MPEG2000，超声分散30min，冷却至常温，密封保存。
6.一种猕猴桃授粉方法，其特征在于：采用权利要求1～4中任意一项所述的猕猴桃授粉液与花粉混合后，通过喷雾器向猕猴桃雌株的花朵上进行喷洒授粉。
7.如权利要求6所述的猕猴桃授粉方法，其特征在于：猕猴桃授粉液与花粉的体积质量比为：100:1～2。
8.如权利要求6所述的猕猴桃授粉方法，其特征在于：按每株猕猴桃雌株0.1mL～0.2mL的进行喷洒授粉。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211170275.0A CN115413666B (zh) | 2022-09-26 | 2022-09-26 | 一种猕猴桃授粉液及制备方法和猕猴桃授粉方法 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211170275.0A CN115413666B (zh) | 2022-09-26 | 2022-09-26 | 一种猕猴桃授粉液及制备方法和猕猴桃授粉方法 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115413666A CN115413666A (zh) | 2022-12-02 |
| CN115413666B true CN115413666B (zh) | 2024-03-12 |
Family
ID=84203622
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211170275.0A Active CN115413666B (zh) | 2022-09-26 | 2022-09-26 | 一种猕猴桃授粉液及制备方法和猕猴桃授粉方法 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115413666B (zh) |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114521484A (zh) * | 2020-11-23 | 2022-05-24 | 成都新朝阳作物科学股份有限公司 | 含14-羟基芸苔素甾醇的授粉液及其制备方法和应用 |
-
2022
- 2022-09-26 CN CN202211170275.0A patent/CN115413666B/zh active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114521484A (zh) * | 2020-11-23 | 2022-05-24 | 成都新朝阳作物科学股份有限公司 | 含14-羟基芸苔素甾醇的授粉液及其制备方法和应用 |
Non-Patent Citations (1)
| Title |
|---|
| 基于蛋白质组解析大球盖菇多糖促进猕猴桃生长及抗高温的分子机制;肖文斐 等;核农学报;全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115413666A (zh) | 2022-12-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Nairn et al. | Identification of an agar constituent responsible for hydric control in micropropagation of radiata pine | |
| DE19608918A1 (de) | Nucleinsäuremoleküle, die neue Debranching-Enzyme aus Mais codieren | |
| DE19619918A1 (de) | Nucleinsäuremoleküle codierend lösliche Stärkesynthasen aus Mais | |
| DE19601365A1 (de) | Nucleinsäuremoleküle aus Pflanzen codierend Enzyme, die an der Stärkesynthese beteiligt sind | |
| CN107155739B (zh) | 一种腐殖酸免套膜袋及其制备方法和使用方法 | |
| Berthon et al. | Endogenous levels of plant hormones during the course of adventitious rooting in cuttings of Sequoiadendron giganteum (Lindl.) in vitro | |
| CN113667360A (zh) | 一种腐植酸型大枣防裂果膜剂及其制备方法和应用 | |
| CN112830854A (zh) | 一种防控马铃薯镰刀菌病害的叶面肥及其应用 | |
| CN115413666B (zh) | 一种猕猴桃授粉液及制备方法和猕猴桃授粉方法 | |
| CN115028496A (zh) | 一种耐盐碱植物生长促进剂及其制备方法和应用 | |
| DE68916407T2 (de) | Zuchtverfahren für pflanzliche gewebe. | |
| Browers et al. | Transmission of gross chromosomal variability from suspension cultures into regenerated celery plants | |
| CN110506628B (zh) | 一种阳春砂仁液体授粉方法 | |
| Picard et al. | Wheat (Triticum aestivum): in vitro production and utilization of doubled haploids | |
| White | The influence of pollination on the respiratory activity of the gynaeceum | |
| EP0134536B1 (de) | Verfahren zur Erhaltung und Vermehrung von defekten, nicht-infektiösen Virusgenomen | |
| HU189407B (en) | Process for producing proliferating plant cell-formations | |
| CN114451299A (zh) | 一种花粉形成能力高的毛花猕猴桃父本创制及其应用 | |
| Fedak et al. | Production of trigeneric (barley× wheat)× rye hybrids | |
| CN1129359C (zh) | 一种提高烟叶含钾量的植物生长调节剂及其应用 | |
| Gyllenberg et al. | The" Rhizosphere Effect" of Graminaceous Plants in Virgin Soils III. Comparison with the Effect of Other Plants. | |
| CN105145348A (zh) | 一种烤烟不育系新品种的选育及繁殖方法 | |
| CN115191452B (zh) | 一种复合花粉介质、万寿菊复合介质花粉及其制备方法和应用 | |
| CN112841192A (zh) | 一种猕猴桃授粉液及制备方法和猕猴桃授粉方法 | |
| Vanzulli et al. | Caryological stability of Datura innoxia calli analysed by cytophotometry for 22 hormonal combinations |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |