CN115413531B - Culture material for needle mushroom culture and needle mushroom culture method - Google Patents
Culture material for needle mushroom culture and needle mushroom culture method Download PDFInfo
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- CN115413531B CN115413531B CN202211226131.2A CN202211226131A CN115413531B CN 115413531 B CN115413531 B CN 115413531B CN 202211226131 A CN202211226131 A CN 202211226131A CN 115413531 B CN115413531 B CN 115413531B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
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- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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Abstract
The invention relates to the technical field of flammulina velutipes cultivation, and discloses a flammulina velutipes-based cultivation material and a flammulina velutipes cultivation method, wherein the cultivation material comprises the following components: 35-40% of wood chips; bran 15-20%; corn cob, 25-30%; 10-20% of bean skin; 10-15% of brewer's grains; selenium-carrying microsphere with 10-20% selenium content and 0.0055-0.04%; the water content of the culture material is 61% -71%. The selenium-carrying microsphere with the slow release function is prepared and added into the needle mushroom culture material, so that selenium element is slowly released into the culture material in the whole cultivation period to provide nutrients containing inorganic selenium for the needle mushrooms, and the inorganic selenium is converted into polysaccharide selenium and protein selenium by the metabolism of substances in cells, so that the organic selenium content in the needle mushroom product is improved; and the cultivation experiment proves that: the selenium content in the needle mushroom fruiting body and the selenium content in the culture medium are in positive correlation.
Description
Technical Field
The invention relates to the technical field of needle mushroom cultivation, in particular to a needle mushroom cultivation-based cultivation material and a needle mushroom cultivation method.
Background
The flammulina velutipes is commonly called as broussonetia, frozen fungi, agaricus, winter mushrooms, golden mushrooms and tricholoma lobayense, has delicious taste and rich nutrition, contains 2.72g of protein, 0.13g of fat, 5.45g of sugar, 1.77g of crude fiber, 0.83g of ash, 1.48mg of phosphorus, 0.37mg of potassium, 0.22mg of iron, 0.097mg of calcium, 0.22mg of sodium and 2.27mg of vitamin C, is rich in various amino acids necessary for human bodies, especially has higher lysine and arginine content, is first of the potential mushrooms, and is known as 'intelligent mushrooms' and 'intelligent mushrooms' because children eat the flammulina velutipes to promote the intelligence development and healthy growth of the flammulina velutipes. In addition, the flammulina velutipes also contains substances such as plain mushroom extract, polysaccharide and the like with various physiological activities and pharmacological actions, and is a famous functional nutritional health-care food.
The trace element selenium is a kind of essential element for human body, and is combined with enzymes in the body to generate macromolecular compounds with biological characteristics, and the macromolecular compounds can protect the body from oxidation damage and strengthen immunity. Since selenium cannot be synthesized by the human body, it is required to be taken from the outside.
The needle mushroom product rich in organic selenium, which has no toxic or side effect on human body, is developed, the selenium level of food chain is improved, and the regulation and control of selenium nutrition from the source is an important way for supplementing selenium elements of human body.
Disclosure of Invention
The immobilized lipase Novozym-435 is used for catalyzing esterification of aliphatic olefine acid and glucose in an organic solvent to synthesize an alkenylated sugar ester, potassium persulfate is used for initiating synthesis of a glucose-based polymer, the glucose-based polymer and sodium alginate are used as matrix materials through a complex coacervation method, sodium selenite is wrapped to prepare selenium-carrying microspheres, the selenium-carrying microspheres are added into flammulina velutipes culture materials, nutrients in the culture materials are absorbed in the flammulina velutipes mycelia growing process, inorganic selenium enters cells, and substances in the cells are metabolized to combine the flammulina velutipes culture materials with macromolecular active substances and are converted into polysaccharide selenium and protein selenium, so that the organic selenium content in flammulina velutipes products is improved;
in order to achieve the above purpose, the present invention provides the following technical solutions:
the invention provides a flammulina velutipes-based culture material for culture, which comprises the following components: 35-40% of wood chips; bran 15-20%; corn cob, 25-30%; 10-20% of bean skin; 10-15% of brewer's grains; selenium-carrying microsphere with 10-20% selenium content and 0.0055-0.04%; the water content of the culture material is 61% -71%;
the preparation method of the selenium-loaded microsphere comprises the following steps:
step one, glucose and 5-hexenoic acid are taken and dissolved in butanone, lipase Novozym-435 is added in a reaction system, molecular sieve is added, the mixture is reacted on a shaking table, the solvent is evaporated to dryness after filtration, and the mixture is separated and purified by a silica gel chromatographic column to obtain alkenylation sugar ester;
step two, purified water is filled in a sealed polymerization bottle, potassium persulfate and alkenylation sugar ester are added at the same time, nitrogen is introduced, and the mixture is placed in a constant-temperature shaking table for reaction, filtered and dried in vacuum to obtain a glucose-based polymer;
dissolving sodium alginate in a beaker filled with distilled water, stirring until the sodium alginate is dissolved into a transparent colloid solution, adding liquid paraffin and Tween 80, and stirring and standing to form water/oil type emulsion;
and fourthly, dissolving the glucose-based polymer in methanol, adding sodium selenite to prepare a solution containing sodium selenite and the glucose-based polymer, regulating the pH value, dropwise adding the solution into the water/oil-type emulsion under stirring, continuing stirring after dripping, adding glutaraldehyde for solidification, adding n-butanol, fully stirring, standing, centrifuging to separate out a precipitate, washing with distilled water, and vacuum drying to obtain the selenium-carrying microspheres.
The invention provides a flammulina velutipes cultivation method based on a flammulina velutipes cultivation material, which comprises the following steps:
step one, mixing raw materials according to a culture material for culturing flammulina velutipes;
step two, bottling by using a full-automatic bottling machine, wherein the bottling is required to be uniform and the compactness is moderate; wherein the water content of the culture material is 61% -71%;
step three, high-pressure sterilization is adopted, and the temperature is kept for 1h at 110-120 ℃ so as to kill all microorganisms and spores in the raw materials, and the water content of the sterilized culture material is 60-70%;
step four, pushing the sterilizing vehicle into a cooling chamber after sterilization is finished, controlling the temperature of the cooling chamber to be 13-15 ℃, and purifying the cooling chamber by using air;
step five, inoculating by adopting an automatic inoculating machine at 20 ℃, placing the inoculating machine in a fully clean inoculating chamber, conveying the inoculating machine to the outside of the inoculating chamber through a conveying belt after inoculating, and transferring the inoculating machine into a culture chamber for culturing;
step six, controlling the temperature of the culture chamber to be 14-18 ℃ and the humidity to be 70-85% and CO 2 The concentration is 2500-3500 mu L/L, and the needle mushroom cultivation time is 20-25 d;
step seven, after the culture is finished, carrying out fungus scratching treatment, removing surface old strains, and carrying out water treatment after fungus scratching; wherein, the water injection rate (7-10) mL/bottle, the water content on the surface of the bottle mouth is controlled to be 60-70%, and the bottle after bacterial scratching is carried on the bed frame of the cultivation room;
and step eight, bud forcing and fertility are carried out after the fungus scratching until harvesting.
Preferably, the specific method for promoting bud growth is as follows:
after 1-3 d of scratching, the temperature of the cultivation room is kept at 14-15 ℃, the humidity is 97-98%, and CO 2 The concentration is 2500-3000 mu L/L, so that the mycelium on the surface can be recovered rapidly, but the mycelium is not too dense;
after 4-7 d of scratching, the temperature of the cultivation room is kept at 14-15 ℃, the humidity is kept at 85-95%, and CO 2 The concentration is 1500-2000 mu L/L, and primordia starts to form;
10d after scratching, wet fertility roomThe temperature is 95 to 97 percent, the small buds are distributed on the material surface part, the plant fluorescent lamp is used for illumination, when the small buds grow to be 5mm away from the bottle mouth, the temperature is kept at 5 to 10 ℃, and the CO 2 The concentration is 4000-7000 mu L/L, the illumination of the plant fluorescent lamp is 2 hours, and the illumination intensity is controlled at 200-400 Lux;
17d after scratching, control of fertility Chamber CO 2 The concentration is 10000-15000 mu L/L until harvest.
Compared with the prior art, the invention has the following beneficial technical effects:
the selenium-carrying microsphere with the slow release function is prepared by the method, and is added into the needle mushroom culture material, so that selenium element is slowly released into the culture material in the whole cultivation period to provide nutrients containing inorganic selenium for the needle mushrooms, and the inorganic selenium is converted into polysaccharide selenium and protein selenium by the metabolism of substances in cells, so that the organic selenium content in the needle mushroom product is improved.
And the cultivation experiment proves that: the selenium content in the needle mushroom fruiting body and the selenium content in the culture material form positive correlation, when the adding amount of the selenium-loaded microspheres (18.3% of selenium element content) in the culture material is within the range of 0.02%, the biological enrichment factor of the needle mushroom fruiting body on selenium reaches 0.51, the organization rate reaches 86%, and the single yield per bottle is not obviously different from the single yield per bottle of the non-added selenium-loaded microspheres.
Detailed Description
The technical solutions of the embodiments of the present invention will be clearly and completely described below in conjunction with the embodiments of the present invention, and it is apparent that the described embodiments are only some embodiments of the present invention, not all embodiments. Embodiments and features of embodiments in this application may be combined with each other without conflict. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The reagents used in the examples below are all commercially available from manufacturers routine in the art; the experimental methods used are all conventional experimental methods known to the person skilled in the art.
The needle mushroom test strain is platinum No. 1 and is derived from the scientific edible fungi institute in Gaoyou city of Jiangsu province.
Example 1
The invention provides a flammulina velutipes-based culture material for culture, which comprises the following components: the cultivation raw material is wood dust and 38%; bran, 18%; corncob, 28%; bean skin, 15%; brewer's grain, 13%; selenium-carrying microspheres with 18.3 percent of selenium content and 0.002 percent of selenium-carrying microspheres; the water content of the culture medium is 65%.
The preparation procedure of the selenium-loaded microsphere in example 1 is as follows:
dissolving 5.4g of glucose and 6.8g of 5-hexenoic acid in 100mL of butanone, adding 1g of lipase Novozym-435 into a reaction system, adding 38g of molecular sieve, reacting for 48 hours on a shaking table at 40 ℃ and 200r/min, filtering, evaporating the solvent, and separating and purifying by a silica gel chromatographic column to obtain alkenylation sugar ester;
200mL of purified water is filled in a 250mL sealed polymerization bottle, 30mg of potassium persulfate and 228mg of alkenylation sugar ester are added simultaneously, nitrogen is introduced for 30min, the mixture is placed in a constant temperature shaking table at 60 ℃ for reaction for 12h, and the glucose-based polymer is obtained through filtration and vacuum drying;
dissolving 300mg of sodium alginate in a beaker filled with 50mL of distilled water, stirring until the sodium alginate is dissolved into a transparent colloid solution, adding 50mL of liquid paraffin and 20 drops of Tween 80, stirring at 25 ℃ for 30min at 200r/min, and standing to form water/oil emulsion;
300mg of glucose-based polymer is dissolved in 50mL of methanol, 400mg of sodium selenite is added to prepare a solution containing sodium selenite and glucose-based polymer, the pH value is regulated to 3, the solution is dropwise added into the water/oil emulsion under stirring, after the solution is completely dripped, stirring is continued for 30min, 5mL of glutaraldehyde is added to solidify for 30min, 150mL of n-butanol is added, the solution is fully stirred and placed for 30min, precipitate is centrifugally separated at 3000r/min, and the solution is washed by distilled water and then dried in vacuum at 35 ℃ to obtain the selenium-carrying microsphere.
Comparative example 1
The invention provides a flammulina velutipes-based culture material for culture, which comprises the following components: the cultivation raw material is wood dust and 38%; bran, 18%; corncob, 28%; bean skin, 15%; brewer's grain, 13%; the water content of the culture medium is 65%.
Example 2
A needle mushroom cultivation method based on a culture material for needle mushroom cultivation comprises the following steps:
step one, mixing raw materials according to the culture medium for needle mushroom culture in example 1;
step two, bottling by using a full-automatic bottling machine, wherein the bottling is required to be uniform and the compactness is moderate; wherein, the loading amount of the plastic bottle is 1kg, and the water content of the culture material is 65%;
step three, high-pressure sterilization is adopted, the temperature is maintained at 115 ℃ for 1h, so that all microorganisms and spores in the raw materials are killed, and the water content of the sterilized culture material is 64%;
step four, pushing the sterilizing vehicle into a cooling chamber after sterilization is finished, controlling the temperature of the cooling chamber to be 14 ℃, and purifying the cooling chamber by using air;
step five, inoculating by adopting an automatic inoculating machine at 20 ℃, placing the inoculating machine in a fully clean inoculating chamber, conveying the inoculating machine to the outside of the inoculating chamber through a conveying belt after inoculating, and transferring the inoculating machine into a culture chamber for culturing;
step six, controlling the temperature of the culture chamber to 15 ℃, the humidity to 75% and the CO 2 The concentration is 2800 mu L/L, and the needle mushroom cultivation time is 24d;
step seven, after the culture is finished, carrying out fungus scratching treatment, removing surface old strains, and carrying out water treatment after fungus scratching; wherein the water injection amount is 8 mL/bottle, the water content on the surface of the bottle mouth is controlled to be 65%, and the bottle after bacterial scratching is carried on a bed frame of a cultivation room;
step eight, after 2d of scratching, maintaining the temperature of the cultivation room at 15 ℃ and the humidity at 98 percent and CO 2 The concentration is 2800 mu L/L, so that the surface hyphae are quickly recovered, but the hyphae are not too dense;
5d after scratching, keeping the temperature of the cultivation room at 15 ℃ and the humidity at 90% and CO 2 The concentration is 1800 mu L/L, and primordia are formed;
9-12 d after scratching bacteria, the humidity of the breeding room is 96%, the distribution of buds on the material surface is ensured, and simultaneously, the buds are irradiated by a plant fluorescent lamp, and the temperature is kept when the buds grow to be 5mm away from the bottle mouthAt 8 ℃, CO 2 The concentration is 5000 mu L/L, the illumination of a plant fluorescent lamp is carried out for 2 hours, and the illumination intensity is controlled at 300Lux;
17d after scratching, control of fertility Chamber CO 2 The concentration is 13000 mu L/L until harvest.
Comparative example 2:
a needle mushroom cultivation method based on a culture material for needle mushroom cultivation comprises the following steps:
step one, mixing raw materials according to a culture material for culturing needle mushrooms in comparative example 1;
see example 2 for the remaining steps.
Results and analysis:
1. the single yields of flammulina velutipes in example 2 and comparative example 2 were calculated according to the formula [ single yield per bottle (g) = (total harvest quality-amount of fungus in warehouse)/number of cultivation bottles ], and the results are shown in table 1 below.
2. Selenium content determination
(1) Determination of total selenium content: accurately weighing flammulina velutipes fruiting body powder 0.3000g (0.0001 g accurate) in a microwave digestion tube, adding 8mL of nitric acid, placing a gasket, screwing a tank cover, placing in a CEM digestion system for microwave digestion treatment (temperature 180 ℃, pressure 300MPa, power 1000W and time 30 min), taking out the digestion tube after the procedure is finished, expelling acid, fixing the volume to 50mL, preparing a standard curve solution, and detecting the total selenium content in the sample liquid by using an inductively coupled plasma mass spectrometer. And calculating the biological enrichment factor of the flammulina velutipes according to a formula [ biological enrichment factor= [ fruiting body selenium content/(mg/kg) ]/[ culture material selenium content/(mg/kg) ] ].
(2) Determination of inorganic selenium content: accurately weighing needle mushroom powder 0.5000g (0.0001 g) in a 50mL centrifuge tube, adding 30mL of ultrapure water, extracting by ultrasonic wave for 20min, oscillating for 10min in a water bath at 80 ℃, cooling, centrifuging for 10min at 10000r/min, transferring the supernatant to a separating funnel, extracting repeatedly with cyclohexane for 3 times, and separating out water phase to obtain inorganic selenium extract. Concentrating inorganic selenium extract to 4-7 mL, transferring to digestion tube, adding 10mLHNO 3 And detecting the inorganic selenium content in the sample liquid by using an inductively coupled plasma mass spectrometer.
(3) And (3) measuring the content of organic selenium: calculating the organic selenium content in the flammulina velutipes powder according to a formula [ organic selenium content (mg/kg) =total selenium content/(mg/kg) -inorganic selenium content/(mg/kg);
and calculating the selenium organized rate according to the formula [ organized rate/% = [ organic selenium content/(mg/kg) ×100]/[ total selenium content/(mg/kg) ].
The above measurement results are shown in tables 1 and 2 below; wherein, table 1 is the measurement data of single yield and total selenium content of flammulina velutipes cultivated by adding and not adding selenium-carrying microspheres into the cultivation material; table 2 shows the measurement data of total selenium content, biological enrichment factor, inorganic selenium content, organic selenium content and selenium rate of flammulina velutipes cultivated by adding selenium-carrying microspheres into the cultivation material.
TABLE 1
| Example 2 (addition of selenium-carrying microspheres) | Comparative example 2 (selenium microsphere without added) | |
| Single output per bottle (g) | 455 | 458 |
| Total selenium content (mg/kg) | 22.95 | 0.02 |
TABLE 2
| Example 2 | |
| Total selenium content (mg/kg) | 22.95 |
| Biological enrichment factor | 0.51 |
| Inorganic selenium content (mg/kg) | 3.11 |
| Organic selenium content (mg/kg) | 19.84 |
| Selenium organization Rate (%) | 86 |
Claims (9)
1. A flammulina velutipes-based culture medium, which is characterized by comprising: 35-40% of wood chips; bran 15-20%; corn cob, 25-30%; 10-20% of bean skin; 10-15% of brewer's grains; selenium-carrying microsphere with 10-20% selenium content and 0.0055-0.04%; the water content of the culture material is 61% -71%;
the immobilized lipase Novozym-435 is used for catalyzing esterification of aliphatic olefine acid and glucose in an organic solvent to synthesize an alkenylated sugar ester, potassium persulfate is used for initiating synthesis of a glucose-based polymer, and sodium selenite is coated by a complex coacervation method by taking the glucose-based polymer and sodium alginate as matrix materials to prepare the selenium-carrying microsphere, and the preparation method comprises the following steps:
step one, glucose and 5-hexenoic acid are taken and dissolved in butanone, lipase is added into a reaction system, molecular sieve is added, the mixture is reacted on a shaking table, the solvent is evaporated to dryness after filtration, and the mixture is separated and purified by a silica gel chromatographic column to obtain alkenylation sugar ester;
step two, purified water is filled in a sealed polymerization bottle, potassium persulfate and alkenylation sugar ester are added at the same time, nitrogen is introduced, and the mixture is placed in a constant-temperature shaking table for reaction, filtered and dried in vacuum to obtain a glucose-based polymer;
dissolving sodium alginate in a beaker filled with distilled water, stirring until the sodium alginate is dissolved into a transparent colloid solution, adding liquid paraffin and tween, and stirring and standing to form water/oil type emulsion;
and fourthly, dissolving the glucose-based polymer in methanol, adding sodium selenite to prepare a solution containing sodium selenite and the glucose-based polymer, regulating the pH value, dropwise adding the solution into the water/oil-type emulsion under stirring, continuing stirring after dripping, adding glutaraldehyde for solidification, adding n-butanol, fully stirring, standing, centrifuging to separate out a precipitate, washing with distilled water, and vacuum drying to obtain the selenium-carrying microspheres.
2. The flammulina velutipes-based culture medium according to claim 1, wherein the selenium-carrying microspheres have a selenium content of 18.3%.
3. A needle mushroom based culture medium according to claim 1, wherein the culture medium comprises: wood chips, 38%; bran, 18%; corncob, 28%; bean skin, 15%; brewer's grain, 13%; selenium-carrying microspheres with 18.3 percent of selenium content and 0.002 percent of selenium-carrying microspheres; the water content of the culture medium is 65%.
4. A method for cultivating flammulina velutipes based on a compost for cultivation of flammulina velutipes as claimed in any one of claims 1 to 3, comprising the steps of:
step one, mixing raw materials according to a culture material for culturing flammulina velutipes;
step two, bottling by using a full-automatic bottling machine, wherein the bottling is required to be uniform and the compactness is moderate; wherein the water content of the culture material is 61% -71%;
step three, high-pressure sterilization is adopted, and the temperature is kept at 110-120 ℃ for 1h so as to kill all microorganisms and spores in the raw materials, and the water content of the sterilized culture material is 60% -70%;
step four, pushing the sterilizing vehicle into a cooling chamber after sterilization is finished, controlling the temperature of the cooling chamber to be 13-15 ℃, and purifying the cooling chamber by using air;
step five, inoculating by adopting an automatic inoculating machine at 20 ℃, placing the inoculating machine in a fully clean inoculating chamber, conveying the inoculating machine to the outside of the inoculating chamber through a conveying belt after inoculating, and transferring the inoculating machine into a culture chamber for culturing;
step six, controlling the temperature of the culture chamber to be 14-18 ℃, the humidity to be 70-85% and the CO to be controlled 2 The concentration is 2500-3500 mu L/L, and the needle mushroom cultivation time is 20-25 d;
step seven, after the culture is finished, carrying out fungus scratching treatment, removing surface old strains, and carrying out water treatment after fungus scratching; wherein, the water injection rate (7-10) mL/bottle, the water content on the surface of the bottle mouth is controlled to be 60% -70%, and the bottle after bacterial scratching is carried on a bed frame of a cultivation room;
and step eight, bud forcing and fertility are carried out after the fungus scratching until harvesting.
5. The method for cultivating flammulina velutipes according to claim 4, wherein the bud forcing and growing method comprises the following steps: after 1-3 d of scratching, maintaining the temperature of the cultivation room at 14-15 ℃ and the humidity at 97-98% and CO 2 The concentration is 2500-3000 mu L/L.
6. The method for cultivating flammulina velutipes according to claim 4, wherein the bud forcing and growing method comprises the following steps: after 4-7 d of scratching, keeping the temperature of the cultivation room at 14-15 ℃ and the humidity at 85-95% and CO 2 The concentration is 1500-2000 mu L/L.
7. The method for cultivating flammulina velutipes according to claim 4, wherein the bud forcing and growing method comprises the following steps: after scratching the bacteria for 9-12 days, the humidity of the breeding room is 95% -97%, and the plant fluorescent lamp is used for illumination until the buds grow to be 5mm away from the bottle mouthThe temperature is kept at 5-10 ℃ and CO 2 The concentration is 4000-7000 mu L/L.
8. The method for cultivating flammulina velutipes according to claim 7, wherein the flammulina velutipes is illuminated by a plant fluorescent lamp for 2 hours, and the illumination intensity is controlled to be 200-400 Lux.
9. The method for cultivating flammulina velutipes according to claim 4, wherein the bud forcing and growing method comprises the following steps: 17d after scratching, control of fertility Chamber CO 2 The concentration is 10000-15000 mu L/L until harvest.
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| JP6548124B2 (en) * | 2015-09-01 | 2019-07-24 | 学校法人甲南学園 | A culture solution for mulberry cultivation, a culture medium for mulberry cultivation, a method for producing a culture liquid for mulberry cultivation, and a cultivation method for mulberry |
| CN105453894A (en) * | 2015-11-30 | 2016-04-06 | 江苏康盛农业发展有限公司 | Method for high-yield of bottle-cultivated golden mushroom |
| CN109076879A (en) * | 2018-11-03 | 2018-12-25 | 上海品柔文化发展有限公司 | A kind of selenium-rich gold needle mushroom and its production method |
| CN109566264A (en) * | 2019-01-18 | 2019-04-05 | 上海市农业科学院 | A kind of cultural method and cultivation matrix of Se-rich xianggu |
| CN110511297A (en) * | 2019-10-14 | 2019-11-29 | 武汉华联科生物技术有限公司 | A kind of extraction separation and purification method of selenium lentinan |
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