CN115404153A - Cell culture exosome collection device - Google Patents
Cell culture exosome collection device Download PDFInfo
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- CN115404153A CN115404153A CN202211352895.6A CN202211352895A CN115404153A CN 115404153 A CN115404153 A CN 115404153A CN 202211352895 A CN202211352895 A CN 202211352895A CN 115404153 A CN115404153 A CN 115404153A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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Abstract
The invention belongs to the technical field of cell culture, and particularly relates to a cell culture exosome collecting device which comprises a bottom plate and a collecting mechanism arranged on the surface of the bottom plate, wherein the collecting mechanism comprises an air inlet assembly, a jacking assembly, a limiting assembly, a dismounting assembly and a collecting assembly; the device is favorable for enabling the supernatant to flow into the inner collecting cup at a constant speed for collection, realizes control over the collected amount, is favorable for conveniently taking out the collected supernatant from the inner part of the round outer sealing cup, and improves the convenience in the use process of the device.
Description
Technical Field
The invention belongs to the technical field of cell culture, and particularly relates to a cell culture exosome collecting device.
Background
The exosome is a small membrane vesicle (30-150 nm) containing complex RNA and protein, and nowadays, the exosome refers to a disc-shaped vesicle with the diameter of 40-100nm, various cells can secrete the exosome under normal and pathological states, the exosome mainly comes from a multivesicular body formed by invagination of intracellular lysosome particles, the multivesicular body is released into an extracellular matrix after fusion of an outer membrane and a cell membrane of the multivesicular body, and all cultured cell types can secrete the exosome;
in the prior art, exosome needs to be sucked out of supernatant liquid of cultured cells and then moved into a separating device, most of suction devices are in a traditional mode that air bags are matched with test tubes, the air bags need to be repeatedly squeezed, air pressure cannot be controlled, the suction amount cannot be controlled, the collection cannot be completed in the suction amount, and the cells are easily sucked into a collecting tube in the suction amount.
For this reason, a cell culture exosome collecting device was designed to solve the above-described problems.
Disclosure of Invention
To solve the problems set forth in the background art described above. The cell culture exosome collecting device provided by the invention is beneficial to enabling supernatant to flow into the inner collecting cup at a constant speed for collection, realizing control over the collection amount, facilitating the collected supernatant to be taken out from the inner part of the round outer sealing cup, and improving the convenience of the device in the using process.
In order to achieve the purpose, the invention provides the following technical scheme: a cell culture exosome collecting device comprises a bottom plate and a collecting mechanism arranged on the surface of the bottom plate;
collect the mechanism including admitting air subassembly, jacking subassembly, spacing subassembly, dismantlement subassembly and collection subassembly, the subassembly setting of admitting air is in the upper surface of bottom plate, the jacking subassembly sets up the surface of the subassembly of admitting air, spacing unit mount is in the upper surface of bottom plate, and pass through the jacking subassembly with the subassembly that admits air is connected, the collection subassembly sets up the surface of spacing subassembly, the dismantlement subassembly sets up the bottom surface of spacing subassembly.
Preferably, the cell culture exosome collecting device comprises a motor, an engaging plate, a threaded rod, a threaded sleeve, connecting plates, circular rubber discs and a sealing air cylinder, wherein the engaging plate is arranged above the bottom plate, the motor is arranged on the upper surface of the engaging plate, the threaded rod penetrates through the surface of the engaging plate and is connected with an output shaft of the motor, the threaded rod is rotatably connected with the engaging plate, the threaded sleeve is in threaded connection with the surface of the threaded rod and is fixedly connected with the bottom plate, the two connecting plates are symmetrically and fixedly connected to one surface of the engaging plate close to the bottom plate, the circular rubber discs are fixedly connected to one ends of the connecting plates far away from the engaging plate, the sealing air cylinder is in sliding connection with the surface of the circular rubber discs and is fixedly connected with the bottom plate, and the sealing air cylinder is positioned on the right side of the threaded sleeve.
Preferably, the air inlet assembly further comprises a positioning rod, the positioning rod is connected between the two connecting plates in a sliding manner, and the positioning rod is fixedly connected inside the sealing air cylinder.
Preferably, the limiting assembly comprises an upper cover, a connecting disc, a round outer sealing cup and a rubber plug, the round outer sealing cup is mounted on the upper surface of the bottom plate, the upper cover is sleeved on the surface of the round outer sealing cup and is in threaded connection with the round outer sealing cup, the connecting disc is rotatably connected in a round mounting groove formed in the surface of the upper cover, and the rubber plug is inserted into the round outer sealing cup and is fixedly connected with the connecting disc.
Preferably, the limiting component further comprises balls, an outer raceway is formed on the inner wall surface of the circular mounting groove, an inner raceway is formed on the surface of the connecting disc, which is close to the outer raceway, and the balls in the annular array are rotatably connected to the inner raceway and the inner raceway.
Preferably, the jacking assembly comprises a first air pipe, a first cap, a three-way pipe, a second cap, a second air pipe, a first valve and a spring air bag, wherein one end of the first air pipe is fixedly connected with the first cap, one end of the second air pipe is fixedly connected with the second cap, two ends of the three-way pipe are respectively inserted into the first cap and the second cap, one end of the first air pipe, far away from the first cap, is inserted into the surface of the sealing air cylinder and extends into the sealing air cylinder, the first valve is mounted on the surface of the second air pipe, one end of the second air pipe, far away from the second cap, is inserted into the surface of the circular outer sealing cup and extends into the circular outer sealing cup, the spring air bag is inserted into the spring air bag fixedly connected inside the circular outer sealing cup, the spring air bag is communicated with the second air pipe, and the second air pipe is connected with the circular outer sealing cup in a sealing manner.
Preferably, the jacking assembly further comprises a support ring, the support ring is fixedly connected to the inner wall surface of the round outer sealing cup, and the spring air bag is located inside the support ring.
Preferably, the collecting assembly comprises an inner collecting cup, a third air pipe, a plug and a second valve, the inner collecting cup is arranged on the upper surface of the support ring and is positioned inside the round outer sealing cup, the third air pipe is fixedly connected to the upper end of the three-way pipe, the second valve is arranged on the surface of the third air pipe, the plug is fixedly connected to one end, far away from the three-way pipe, of the third air pipe, the plug penetrates through a first round hole formed in the surface of the connecting disc and is inserted into the surface of the rubber plug and communicated with the inside of the round outer sealing cup.
Preferably, the collection assembly further comprises a liquid collection tube, the liquid collection tube penetrates through the second round hole formed in the surface of the adapter disc, is inserted into the surface of the rubber plug, and extends into the inner collection cup.
Preferably, the disassembling assembly comprises a rectangular insertion block, a return spring, an internal block and a triangular block, the rectangular insertion block is fixedly connected to the bottom surface of the round external sealing cup, a rectangular insertion groove matched with the rectangular insertion block is formed in the upper surface of the bottom plate, the rectangular insertion block is inserted into the rectangular insertion groove, the internal grooves are symmetrically formed in two sides of the rectangular insertion block, the return spring and the internal block are located in the internal groove, two ends of the return spring are respectively fixedly connected with the internal block and the rectangular insertion block, the triangular block is fixedly connected to one surface, away from the return spring, of the internal block, triangular insertion grooves matched with the triangular block are symmetrically formed in the inner wall surface of the rectangular insertion groove, and the triangular block is inserted into the triangular insertion groove.
Compared with the prior art, the invention has the beneficial effects that: the device is favorable for enabling the supernatant to flow into the inner collecting cup at a constant speed for collection, realizes controlling the collected amount, is favorable for conveniently taking out the collected supernatant from the inside of the round outer sealing cup, improves the convenience in the use process of the device, facilitates the installation and the disassembly process of the round outer sealing cup, and improves the use effect of the device.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a cross-sectional view of the present invention;
FIG. 3 is an enlarged view taken at A of FIG. 2 in accordance with the present invention;
FIG. 4 is an enlarged view taken at B of FIG. 2 in accordance with the present invention;
FIG. 5 is an enlarged view of the invention at C of FIG. 2;
FIG. 6 is a schematic view of the construction of the threaded sleeve and threaded rod of the present invention;
FIG. 7 is a schematic view showing the construction of the spring bellows and the second air tube in the present invention;
FIG. 8 is a schematic structural view of the built-in block and the return spring of the present invention;
FIG. 9 is a schematic structural view of an upper cover and a rubber stopper in the present invention;
in the figure:
1. a base plate; 2. a collection mechanism;
21. an air intake assembly; 211. a motor; 212. a connector tile; 213. a threaded rod; 214. a threaded sleeve; 215. a connecting plate; 216. positioning a rod; 217. a circular rubber disc; 218. sealing the air cylinder;
22. a jacking assembly; 221. a first air pipe; 222. a first cap; 223. a three-way pipe; 224. a second cap; 225. a second air pipe; 226. a first valve; 227. a spring air bag; 228. a support ring;
23. a limiting component; 231. an upper cover; 232. a splice tray; 233. an inner raceway; 234. a ball bearing; 235. an outer raceway; 236. a rubber plug; 237. a circular mounting groove; 238. an outer sealing cup;
24. disassembling the assembly; 241. a rectangular slot; 242. a rectangular insert block; 243. a return spring; 244. a built-in groove; 245. a built-in block; 246. a triangular block; 247. a triangular slot;
25. a collection assembly; 251. a third air pipe; 252. a second valve; 253. a plug; 254. an inner collection cup; 255. a liquid collecting pipe.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1-9;
a cell culture exosome collecting device comprises a bottom plate 1 and a collecting mechanism 2 arranged on the surface of the bottom plate 1;
collecting mechanism 2 and including air intake assembly 21, jacking assembly 22, spacing subassembly 23, dismantlement subassembly 24 and collection subassembly 25, air intake assembly 21 sets up the upper surface at bottom plate 1, and jacking assembly 22 sets up the surface at air intake assembly 21, and spacing subassembly 23 is installed at bottom plate 1's upper surface to be connected with air intake assembly 21 through jacking assembly 22, collect subassembly 25 and set up the surface at spacing subassembly 23, and dismantlement subassembly 24 sets up the bottom surface at spacing subassembly 23.
In an alternative embodiment, the air intake assembly 21 includes a motor 211, an adapter plate 212, a threaded rod 213, a threaded sleeve 214, a connecting plate 215, a circular rubber disc 217 and a sealing air cylinder 218, the adapter plate 212 is disposed above the base plate 1, the motor 211 is mounted on the upper surface of the adapter plate 212, the threaded rod 213 penetrates through the surface of the adapter plate 212 and is connected to the output shaft of the motor 211, the threaded rod 213 and the adapter plate 212 are rotatably connected, the threaded sleeve 214 is threadedly connected to the surface of the threaded rod 213 and is fixedly connected to the base plate 1, the two connecting plates 215 are symmetrically and fixedly connected to the side of the adapter plate 212 close to the base plate 1, the circular rubber disc 217 is fixedly connected to the end of the connecting plate 215 far from the adapter plate 212, the sealing air cylinder 218 is slidably connected to the surface of the circular rubber disc 217 and is fixedly connected to the base plate 1, and the sealing air cylinder 218 is located on the right side of the threaded sleeve 214.
In an alternative embodiment, the air intake assembly 21 further comprises a positioning rod 216, the positioning rod 216 is slidably connected between the two connecting plates 215, and the positioning rod 216 is fixedly connected inside the sealed air cylinder 218.
In an alternative embodiment, the position limiting assembly 23 includes an upper cover 231, a connecting disc 232, a round outer sealing cup 238 and a rubber plug 236, the round outer sealing cup 238 is installed on the upper surface of the base plate 1, the upper cover 231 is sleeved on the surface of the round outer sealing cup 238 and is in threaded connection with the round outer sealing cup 238, the connecting disc 232 is rotatably connected in a round mounting groove 237 formed on the surface of the upper cover 231, and the rubber plug 236 is inserted into the round outer sealing cup 238 and is fixedly connected with the connecting disc 232.
In an alternative embodiment, the position-limiting component 23 further includes balls 234, an outer raceway 235 is formed on an inner wall surface of the circular mounting groove 237, an inner raceway 233 is formed on a surface of the engaging disc 232 at a position close to the outer raceway 235, and the balls 234 in the annular array are rotatably connected inside the inner raceway 233 and the outer raceway 235.
In an alternative embodiment, the jacking assembly 22 includes a first air pipe 221, a first cap 222, a tee pipe 223, a second cap 224, a second air pipe 225, a first valve 226 and a spring air bag 227, wherein one end of the first air pipe 221 is fixedly connected with the first cap 222, one end of the second air pipe 225 is fixedly connected with the second cap 224, two ends of the tee pipe 223 are respectively inserted into the first cap 222 and the second cap 224, one end of the first air pipe 221, which is far away from the first cap 222, is inserted into the surface of the sealing air cylinder 218 and extends into the sealing air cylinder 218, the surface of the second air pipe 225 is provided with the first valve 226, one end of the second air pipe 225, which is far away from the second cap 224, is inserted into the surface of the round outer sealing cup 238 and extends into the round outer sealing cup 238, and is inserted into the spring air bag 227 fixedly connected inside the round outer sealing cup 238, the spring air bag 227 is communicated with the second air pipe 225, and the round outer sealing cup 238 are connected in a sealing manner.
In an alternative embodiment, the jacking assembly 22 further comprises a support ring 228, the support ring 228 is fixedly attached to an inner wall surface of the round outer seal cup 238, and the spring bladder 227 is located inside the support ring 228.
In an alternative embodiment, the collecting assembly 25 comprises an inner collecting cup 254, a third air tube 251, a plug 253 and a second valve 252, the inner collecting cup 254 is arranged on the upper surface of the support ring 228, the inner collecting cup 254 is positioned inside the round outer sealing cup 238, the third air tube 251 is fixedly connected with the upper end of the tee pipe 223, the second valve 252 is arranged on the surface of the third air tube 251, the plug 253 is fixedly connected with one end of the third air tube 251 far away from the tee pipe 223, and the plug 253 penetrates through a round hole I formed in the surface of the adapter disc 232 and is inserted into the surface of the rubber plug 236 and communicated with the inside of the round outer sealing cup 238.
In an alternative embodiment, the collecting assembly 25 further comprises a liquid collecting tube 255, wherein the liquid collecting tube 255 penetrates through the second circular hole formed on the surface of the connecting disc 232, is inserted into the surface of the rubber plug 236, and extends into the inner collecting cup 254.
In an alternative embodiment, the detaching assembly 24 includes a rectangular insert block 242, a return spring 243, an inner insert block 245 and a triangular block 246, the rectangular insert block 242 is fixedly connected to the bottom surface of the round outer seal cup 238, the upper surface of the base plate 1 is provided with a rectangular slot 241 matched with the rectangular insert block 242, the rectangular insert block 242 is inserted into the rectangular slot 241, the two sides of the rectangular insert block 242 are symmetrically provided with inner slots 244, the return spring 243 and the inner insert block 245 are both located inside the inner slots 244, two ends of the return spring 243 are respectively fixedly connected with the inner insert block 245 and the rectangular insert block 242, the triangular block 246 is fixedly connected to one side of the inner insert block 245 far from the return spring 243, the inner wall surface of the rectangular slot 241 is symmetrically provided with a triangular slot 247 matched with the triangular block 246, and the triangular block 246 is inserted into the triangular slot 247.
In this embodiment: when the device is used, a collector firstly aligns a collecting port of a liquid collecting pipe 255 with supernatant to be sucked, a connecting line of a motor 211 is connected with an external power supply, a controller is operated to start the motor 211, the motor 211 operates to drive a threaded rod 213 to rotate, the threaded rod 213 rotates on the inner wall surface of a threaded sleeve 214, under the action of threads, the threaded rod 213 vertically moves, the threaded rod 213 drives the motor 211 to move in the vertical movement process, the motor 211 moves to drive a connecting plate 212 to move, the connecting plate 212 moves to pull a circular rubber disc 217 to slide on the inner wall surface of a sealing air cylinder 218 through a connecting plate 215, due to the sealing connection of the circular rubber disc 217 and the sealing air cylinder 218, negative pressure is generated inside the sealing air cylinder 218, under the action of a third air pipe 251, negative pressure is generated inside a circular outer sealing cup 238, and the collecting port of the liquid collecting pipe 255 aligns the collected supernatant, the supernatant can be sucked into the liquid collecting pipe 255, flows along the inside of the liquid collecting pipe 255 and flows into the inside of the circular outer sealing cup 238 and falls into the inside of the inner collecting cup 254 for collection, when the supernatant is sucked into the inside of the inner collecting cup 254, the motor 211 drives the threaded rod 213 to rotate at a uniform speed, so that the connecting plate 212 rises at a uniform speed, the connecting plate 212 rises at a uniform speed so that the circular rubber disc 217 slides in the sealed air cylinder 218 at a uniform speed, so that the air pressure inside the circular outer sealing cup 238 tends to be balanced when the supernatant is sucked, when the motor 211 does not operate, the circular rubber disc 217 does not move, at the moment, the air pressure inside the circular outer sealing cup 238 cannot suck the supernatant into the inside of the liquid collecting pipe 255, thereby controlling the collection amount, facilitating the supernatant to flow into the inside of the inner collecting cup 254 at a uniform speed for collection, the collection amount is controlled, after the supernatant is collected, the upper cover 231 is rotated, the upper cover 231 rotates, under the action of the threads, the upper cover 231 can be screwed down from the surface of the round outer sealing cup 238, the upper cover 231 drives the rubber plug 236 to slide in the round outer sealing cup 238 in the rotating process until the rubber plug 236 slides out of the round outer sealing cup 238, similarly, when the rubber plug 236 is inserted into the round outer sealing cup 238, the rubber plug 236 can be tightly inserted into the round outer sealing cup 238 by rotating the upper cover 231, the situation that the rubber plug 236 slides out of the round outer sealing cup 238 due to air pressure is avoided, after the rubber plug 236 is taken out of the round outer sealing cup 238, the second valve 252 is closed to open the first valve 226, at this time, the operation of the motor 211 drives the round rubber disc 217 to move downwards, so that gas is injected into the spring air bag 227, when gas is injected into the spring air bag 227, the spring air bag 227 is expanded, the inner collecting cup 254 is conveniently taken out of the inner part of the round outer sealing cup 238 when the gas is increased in the spring air bag 227, the collected supernatant is conveniently taken out of the inner part of the round outer sealing cup 238, the convenience in using the device is improved, when the round outer sealing cup 238 needs to be detached and cleaned, the acting force is applied to the round outer sealing cup 238, the triangular block 246 can slide on the inner wall surface of the triangular slot 247, the return spring 243 is extruded by the built-in block 245 in the process that the triangular block 246 slides on and slides off the inner wall surface of the triangular slot 247, so that the return spring 243 is in a compressed state, and after the triangular block 246 slides off from the inner part of the triangular slot 247, the rectangular insert block 242 can be taken out from the inside of the rectangular slot 241, so that the round outer seal cup 238 can be conveniently cleaned, similarly, when the position of the round outer seal cup 238 is fixed, the rectangular insert block 242 slides along the inner wall surface of the rectangular slot 241, when the triangular block 246 slides into the inside of the triangular slot 247, under the action of elastic potential energy of the return spring 243, the position of the rectangular insert block 242 is fixed, namely the round outer seal cup 238 is installed, the installation and the disassembly processes of the round outer seal cup 238 are convenient, and the using effect of the device is improved.
It should be noted that: the operation of the motor 211 enables the positioning rod 216 to slide between the two connecting plates 215 during the vertical movement of the connecting plates 215, so as to limit the positions of the connecting plates 215 and avoid the connecting plates 215 from tilting.
It should be noted that: the surface of the round outer sealing cup 238 is inlaid with a thread matched with the inside of the upper cover 231, so that the upper cover 231 is fixed on the round outer sealing cup 238, the rubber plug 236 can be tightly inserted into the inside of the round outer sealing cup 238 by rotating the upper cover 231, the condition that the rubber plug 236 slides out of the inside of the round outer sealing cup 238 due to air pressure is avoided, and the sealing performance of the device in the use process is ensured.
It should be noted that: during the process of collecting the supernatant, the first valve 226 is closed, the second valve 252 is opened, the gas in the sealed gas cylinder 218 flows along the inside of the third gas pipe 251, when the inner collecting cup 254 in the round outer sealing cup 238 needs to be taken out, the second valve 252 is closed, the first valve 226 is opened, and the operation of the motor 211 drives the round rubber disc 217 to move downwards, so that the gas is injected into the spring air bag 227.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described above, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. A cell culture exosome collecting device, includes bottom plate (1), its characterized in that: the device also comprises a collecting mechanism (2) arranged on the surface of the bottom plate (1);
collect mechanism (2) including air intake assembly (21), jacking subassembly (22), spacing subassembly (23), dismantlement subassembly (24) and collection subassembly (25), air intake assembly (21) sets up the upper surface of bottom plate (1), jacking subassembly (22) set up the surface of air intake assembly (21), spacing subassembly (23) are installed the upper surface of bottom plate (1), and pass through jacking subassembly (22) with air intake assembly (21) are connected, it is in to collect subassembly (25) setting the surface of spacing subassembly (23), dismantle subassembly (24) set up the bottom surface of spacing subassembly (23).
2. The cell culture exosome collecting device according to claim 1, characterized in that: the air intake assembly (21) comprises a motor (211), an adapter plate (212), a threaded rod (213), a threaded sleeve (214), a connecting plate (215), a circular rubber disc (217) and a sealing air cylinder (218), the adapter plate (212) is arranged above the base plate (1), the motor (211) is mounted on the upper surface of the adapter plate (212), the threaded rod (213) penetrates through the surface of the adapter plate (212) and is connected with an output shaft of the motor (211), the threaded rod (213) and the adapter plate (212) are rotatably connected, the threaded sleeve (214) is in threaded connection on the surface of the threaded rod (213) and is fixedly connected with the base plate (1), the two connecting plates (215) are symmetrically and fixedly connected on one side of the adapter plate (212) close to the base plate (1), the circular rubber disc (217) is fixedly connected at one end of the connecting plate (215) far away from the adapter plate (212), and the sealing air cylinder (218) is slidably connected on the surface of the circular rubber disc (217) and is fixedly connected with the base plate (1), and the sealing air cylinder (218) is located on the right side of the sealing air cylinder (214).
3. The cell culture exosome collecting device according to claim 2, characterized in that: the air inlet assembly (21) further comprises a positioning rod (216), the positioning rod (216) is connected between the two connecting plates (215) in a sliding mode, and the positioning rod (216) is fixedly connected inside the sealed air cylinder (218).
4. The cell culture exosome collecting device according to claim 2, characterized in that: spacing subassembly (23) include upper cover (231), link up dish (232), circle external seal cup (238) and rubber buffer (236), circle external seal cup (238) are installed the upper surface of bottom plate (1), upper cover (231) cover is established the surface of circle external seal cup (238), and with circle external seal cup (238) threaded connection, link up dish (232) rotate to be connected in circular mounting groove (237) that upper cover (231) surface was seted up, rubber buffer (236) are inserted and are established the inside of circle external seal cup (238), and with link up dish (232) fixed connection.
5. The cell culture exosome collecting device according to claim 4, characterized in that: the limiting assembly (23) further comprises balls (234), an outer raceway (235) is formed in the inner wall surface of the circular mounting groove (237), an inner raceway (233) is formed in the position, close to the outer raceway (235), of the surface of the connecting disc (232), and the balls (234) in an annular array are rotatably connected inside the inner raceway (233) and the outer raceway (235).
6. The cell culture exosome collecting device according to claim 4, characterized in that: the jacking assembly (22) comprises a first air pipe (221), a first cap (222), a three-way pipe (223), a second cap (224), a second air pipe (225), a first valve (226) and a spring air bag (227), one end of the first air pipe (221) is fixedly connected with the first cap (222), one end of the second air pipe (225) is fixedly connected with the second cap (224), two ends of the three-way pipe (223) are respectively inserted into the first cap (222) and the second cap (224), one end of the first air pipe (221), which is far away from the first cap (222), is inserted into the surface of the sealing air cylinder (218) and extends into the interior of the sealing air cylinder (218), the first valve (226) is installed on the surface of the second air pipe (225), one end of the second air pipe (225), which is far away from the second cap (224), is inserted into the surface of the round outer sealing cup (238) and extends into the round outer sealing air cylinder (238), one end of the second air pipe (225) is connected with the spring air bag (227), and the spring air pipe (238) is connected with the second air bag (227).
7. The cell culture exosome collecting device according to claim 6, characterized in that: the jacking assembly (22) further comprises a support ring (228), the support ring (228) is fixedly connected to the inner wall surface of the round outer sealing cup (238), and the spring air bag (227) is located inside the support ring (228).
8. A cell culture exosome collection device according to claim 7, characterised in that: the collecting assembly (25) comprises an inner collecting cup (254), a third air pipe (251), a plug (253) and a second valve (252), the inner collecting cup (254) is arranged on the upper surface of the support ring (228), the inner collecting cup (254) is located inside the round outer sealing cup (238), the third air pipe (251) is fixedly connected to the upper end of the three-way pipe (223), the second valve (252) is installed on the surface of the third air pipe (251), the plug (253) is fixedly connected to one end, away from the three-way pipe (223), of the third air pipe (251), and the plug (253) penetrates through a first round hole formed in the surface of the adaptor disc (232), is inserted into the surface of the rubber plug (236) and is communicated with the inside of the round outer sealing cup (238).
9. The cell culture exosome collection device according to claim 8, characterized in that: the collecting assembly (25) further comprises a liquid collecting pipe (255), the liquid collecting pipe (255) penetrates through a round hole II formed in the surface of the connecting disc (232), is inserted into the surface of the rubber plug (236), and extends to the inner part of the inner collecting cup (254).
10. A cell culture exosome collection device according to claim 4, characterised in that: the dismounting assembly (24) comprises a rectangular insert block (242), a reset spring (243), a built-in block (245) and a triangular block (246), the rectangular insert block (242) is fixedly connected to the bottom surface of the round outer sealing cup (238), the upper surface of the base plate (1) is provided with the rectangular insert block (242) matched with the rectangular insert block (241), the rectangular insert block (242) is inserted in the rectangular insert groove (241), built-in grooves (244) are symmetrically formed in two sides of the rectangular insert block (242), the reset spring (243) and the built-in block (245) are located in the built-in groove (244), two ends of the reset spring (243) are respectively connected with the built-in block (245) and the rectangular insert block (242) in a fixed mode, the triangular block (246) is fixedly connected to the built-in block (243) and far away from the reset spring (244), one surface of the inner wall surface of the rectangular insert groove (241) is symmetrically provided with the triangular block (246) matched with the triangular block (246), and the triangular block (247) is inserted in the triangular insert groove (246).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211352895.6A CN115404153B (en) | 2022-11-01 | 2022-11-01 | Cell culture exosome collection device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211352895.6A CN115404153B (en) | 2022-11-01 | 2022-11-01 | Cell culture exosome collection device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115404153A true CN115404153A (en) | 2022-11-29 |
| CN115404153B CN115404153B (en) | 2023-02-03 |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102010001292A1 (en) * | 2010-01-27 | 2011-07-28 | Human Med AG, 19061 | Device for separation of tissue cells from a liquid, comprises a collecting container forming a collecting chamber, where the collecting container in one hand is connected to vacuum source and the other hand is connected to supply pipe |
| CN210151061U (en) * | 2019-03-29 | 2020-03-17 | 易春 | Semi-automatic exosome purification system |
| CN112159754A (en) * | 2020-10-12 | 2021-01-01 | 广州爱索达生物医药技术有限公司 | Exosome tumor vaccine preparation equipment |
| CN217628424U (en) * | 2022-05-18 | 2022-10-21 | 杨光华 | Novel cell culture device for continuously collecting cell exosomes |
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE102010001292A1 (en) * | 2010-01-27 | 2011-07-28 | Human Med AG, 19061 | Device for separation of tissue cells from a liquid, comprises a collecting container forming a collecting chamber, where the collecting container in one hand is connected to vacuum source and the other hand is connected to supply pipe |
| CN210151061U (en) * | 2019-03-29 | 2020-03-17 | 易春 | Semi-automatic exosome purification system |
| CN112159754A (en) * | 2020-10-12 | 2021-01-01 | 广州爱索达生物医药技术有限公司 | Exosome tumor vaccine preparation equipment |
| CN217628424U (en) * | 2022-05-18 | 2022-10-21 | 杨光华 | Novel cell culture device for continuously collecting cell exosomes |
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