CN115403156A - Method for removing nitrogen and phosphorus from high-ammonia-nitrogen livestock wastewater by using two-stage treatment system - Google Patents
Method for removing nitrogen and phosphorus from high-ammonia-nitrogen livestock wastewater by using two-stage treatment system Download PDFInfo
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- CN115403156A CN115403156A CN202211108288.5A CN202211108288A CN115403156A CN 115403156 A CN115403156 A CN 115403156A CN 202211108288 A CN202211108288 A CN 202211108288A CN 115403156 A CN115403156 A CN 115403156A
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Abstract
The invention belongs to the technical field of biology, and particularly relates to a method for removing nitrogen and phosphorus from high-ammonia nitrogen livestock wastewater by using an efficient two-stage continuous treatment system. The invention utilizes the chlorella sorokiniana and the activated sludge to construct an algae bacteria symbiotic system according to a certain proportion, different carbon sources are added into the algae bacteria symbiotic system, and the actual biogas slurry wastewater is purified by a two-stage treatment method (namely, one-stage algae bacteria symbiotic treatment and two-stage pure microalgae retreatment) under the condition of a certain carbon-nitrogen ratio. The invention adds the activated sludge into the microalgae to promote the removal of pollutants in the wastewater and the growth of microorganisms in an algal-bacteria symbiotic system, and further explores the type and the concentration of an optimal organic carbon source. On the basis, the purification characteristics of actual biogas slurry wastewater are researched by utilizing a two-stage continuous treatment mode, so that the treated biogas slurry wastewater can reach the discharge standard, and theoretical and technical support is provided for actual large-scale treatment.
Description
Technical Field
The invention belongs to the technical field of biology, and particularly relates to a method for removing nitrogen and phosphorus from high-ammonia nitrogen livestock wastewater by using an efficient two-stage continuous treatment system.
Background
In recent years, with the rapid development of economic strength in China, the quality of life needs of people is increasingly improved, and the proportion of meat, protein and milk products in daily diet is increased year by year and tends to be stable. The pork demand is increased year by year, the stable supply of the market is guaranteed, and the livestock and poultry breeding industry in China is also developed towards large-scale and intensification from small-scale decentralized breeding and gradually becomes the prop industry for the economic development of rural areas in China. A large number of large-scale pig farms drive the economic development of rural areas, and meanwhile, the environmental problems are inevitable.
The method for culturing microalgae by utilizing the pig-raising biogas slurry wastewater promotes the development of the microalgae energy industry while realizing water quality purification, is a new application of biotechnology in the direction of environmental remediation, and is gradually recognized in the environmental field. However, the method for culturing microalgae by using pig-raising wastewater does not realize industrialization at present, and related researches still have some problems, mainly focusing on the following aspects: the pig raising biogas slurry wastewater has low C/N and poor biodegradability. The unbalanced C/N is not beneficial to the absorption and transformation of microorganisms on nutrient components in the wastewater, so that the growth and reproduction, metabolic activity and degradation of organic pollutants of microalgae in a system are inhibited, and the treatment effect is not ideal; oxygen (O) is released by photosynthesis during the growth of microalgae 2 ) Dissolved oxygen accumulation is easily caused, the phenomenon of photorespiration can be generated due to overhigh oxygen partial pressure, the yield of microalgae biomass is reduced, the intracellular propylene glycol accumulation is caused, and toxicity is generated on cell metabolism.
Disclosure of Invention
Aiming at the problems in the prior art, the invention provides a method for removing nitrogen and phosphorus from high-ammonia nitrogen livestock wastewater by using an efficient two-stage continuous treatment system (phycobiont and microalgae system). The problems of dissolved oxygen accumulation and excessively low C/N are solved by respectively adding activated sludge to form an phycobiont system and adding a certain amount of organic carbon source, and microalgae biomass energy is obtained while biogas slurry wastewater is treated.
In order to achieve the purpose, the invention comprises the following technical scheme:
a method for removing nitrogen and phosphorus from high ammonia nitrogen livestock wastewater by a two-stage treatment system specifically comprises the following steps:
(1) Culturing the chlorella sofosvenorii in a light incubator by using BG11 culture solution until the culture reaches a logarithmic phase, and performing centrifugal concentration to obtain seed solution;
(2) The first stage treatment: inoculating chlorella sorokiniana liquid and activated sludge into sterilized biogas slurry wastewater, adding an organic carbon source, controlling the carbon-nitrogen ratio, and culturing to obtain a treatment solution;
(3) And (2) second-stage treatment: and centrifuging the first-stage treatment solution at a high speed, collecting supernatant, performing autoclaving on the collected supernatant again to obtain a culture medium for inoculating microalgae, adding a chlorella sorokiniana solution, and continuously performing second-stage advanced treatment on the wastewater to finish the treatment.
Further, the biomass in the seed liquid in the step (1) is 0.4-0.8 g/L; preferably 0.4g/L.
Further, the incubation conditions described in step (1) were 25. + -. 1 ℃ and 4000 lux.
Furthermore, the feeding proportion of the Chlorella sorokiniana to the activated sludge in the step (2) is 1 (1-5) ~ (1-5): 1, calculated as MLSS, w/w.
Further, the activated sludge in the step (2) is organic sludge, preferably aerobic sludge.
Further, the culture conditions in the step (2) are that the illumination intensity is 4000-6000 lux, the culture is carried out under 24h continuous illumination, and the conical flask is shaken for 1-3 min in the morning, at noon and at night every day.
Further, the culturing period in the step (2) is at least 5 days; preferably the treatment time is at least 7 days; more preferably, the treatment time is 7 days.
Further, the carbon source in the step (2) is at least one selected from glucose, sodium acetate, starch and citric acid.
Further, the carbon-nitrogen ratio C/N =2.2-30 in the step (2); preferably C/N =30.
Further, the initial biomass of the chlorella sorokiniana added in the second stage in the step (3) is 0.4-0.8 g/L; preferably 0.4g/L.
Further, the deep treatment in the step (3) is carried out under the conditions that the illumination intensity is 4000-6000 lux, and the culture is carried out under 24h continuous illumination, and the conical flask is shaken for 1-3 min respectively in the morning, at noon and at night every day.
Further, the time of the deep treatment in the step (3) is at least 5 days; preferably the treatment time is at least 7 days; more preferably, the treatment time is 7 days.
Further, sampling 50 mL every 24h in each stage of culture period, determining microbial biomass and wastewater pH, filtering by a 0.45μm filter membrane, and taking filtrate to detect TN, TP and NH in water quality 4 + The concentration of N and COD.
Further, a certain amount of dried algae powder is weighed at the end of the culture period of each stage, and the contents of grease, total sugar and protein accumulated in the microalgae in the two stages are respectively measured.
Furthermore, the biogas slurry wastewater can reach the discharge standard of pollutants for livestock and poultry breeding (GB 18596-2001) after the two-stage treatment method.
Compared with the prior art, the invention has the following effects:
the invention provides a method for removing nitrogen and phosphorus from high-ammonia-nitrogen livestock wastewater by using an efficient two-stage continuous treatment system (phycobiont + microalgae system). By using an activated sludge added to form an phycobiont system and adding a certain amount of organic carbon source, the problems of dissolved oxygen accumulation and excessively low C/N are solved, and microalgae biomass energy is obtained while biogas slurry wastewater is treated; after the two-stage treatment, the pollutants in the pig raising biogas slurry can be discharged up to the standard, and simultaneously, the oil content, the polysaccharide content and the protein content of the accumulated microalgae are more than 31%, more than 25% and more than 41%.
Drawings
FIG. 1 example 4 two-stage treatment of NH in actual pig-raising biogas slurry wastewater 4 + Trends in N (A), TN (B), TP (C) and COD (D).
FIG. 2. Comparative example 1 two-stage treatment of NH in actual pig-raising biogas slurry wastewater 4 + Trends in N (A), TN (B), TP (C) and COD (D).
Detailed Description
The present application is described in further detail below by way of examples to enable those skilled in the art to practice the present application. It is to be understood that other embodiments may be utilized and that changes may be made without departing from the spirit or scope of the present application. To avoid detail not necessary to enable those skilled in the art to practice the application, the description may omit certain information known to those skilled in the art. The following detailed description is, therefore, not to be taken in a limiting sense, and the scope of the present invention is defined only by the appended claims. The following examples are presented to facilitate a better understanding of the present application and are not intended to limit the scope of the present application.
The Chlorella sorokiniana (Chlorella sorokiniana) used in the embodiment of the invention is purchased from a freshwater algae seed bank of China academy of sciences, the activated sludge is taken from a secondary sedimentation tank of a certain water quality purification plant in Changqing district of Jinan City of Shandong, and the actual pig raising biogas slurry wastewater is taken from the effluent of an anaerobic digestion tank of a certain pig farm in Laiyang City of Taiwan city of Yantai city of Shandong.
Example 1
(1) Culturing Chlorella sorokiniana in a light incubator (25 + -1 ℃,4000 lux) by using BG11 culture solution until logarithmic phase, centrifuging and concentrating to obtain seed solution;
(2) Taking simulated pig raising biogas slurry wastewater as a research substrate, respectively placing 500mL of wastewater in a conical flask, setting the initial inoculation amount of a reaction system to be about 0.4g/L, setting the ratio of phycomycetes (measured by MLSS, w/w) to be 1, 3, 1 and 5;
(3) Taking water samples every 24h to determine the biomass of the algae liquid, the TN, the TP and the NH of the wastewater 4 + The concentrations of N and COD are analyzed and simulated the treatment condition of pollutants in the biogas slurry wastewater and the growth condition of microorganisms.
The content data of various pollutants in the wastewater before and after the experiment are shown in table 1, and the treatment effect of the phycomycete ratio of 1 to 3 to the ammonia nitrogen and the total nitrogen in the wastewater is the best, and is 69.33 percent and 69.52 percent respectively.
TABLE 1 comparison of pollutant content before and after wastewater treatment under different phycomycete ratios
Example 2
(1) Culturing Chlorella sorokiniana in a light incubator (25 + -1 ℃,4000 lux) by using BG11 culture solution until logarithmic phase, centrifuging and concentrating to obtain seed solution;
(2) Keeping the initial inoculation amount of the reaction system to be 0.4g/L, and the ratio of the added algae to be 1: and 3, adding glucose as an organic carbon source, adjusting the C/N to be 2.2, 5, 10, 15 and 30 respectively, and culturing under 4000 lux and 24h continuous illumination for 7 days.
(3) And (4) taking water samples every 24 hours to determine the concentrations of algae liquid biomass, TN, TP, NH4+ -N and COD of the wastewater, and analyzing the treatment condition of pollutants in the simulated biogas slurry wastewater and the growth condition of microorganisms.
The content data of each pollutant in the wastewater before and after the experiment are shown in table 2, the treatment effect of ammonia nitrogen, total phosphorus and COD in the wastewater is the best when the carbon-nitrogen ratio is 30, and the treatment effect is respectively 96.67 percent, 95.57 percent, 97.02 percent and 92.44 percent.
TABLE 2 detection results of pollutant content before and after wastewater treatment under different carbon-nitrogen ratios
Example 3
(1) Culturing Chlorella sorokiniana in a light incubator (25 + -1 deg.C, 5000 lux) with BG11 culture solution to logarithmic phase, centrifuging, and concentrating to obtain seed solution;
(2) Keeping the initial inoculation amount of the reaction system at 0.4g/L and the ratio of added algae to bacteria at 1.
(3) Taking water samples every 24h to determine the biomass of the algae liquid, the TN, the TP and the NH of the wastewater 4 + The concentrations of N and COD are analyzed and simulated the treatment condition of pollutants in the biogas slurry wastewater and the growth condition of microorganisms.
The content data of various pollutants in the wastewater before and after the experiment are shown in table 3, and the treatment effects of the organic carbon source sodium acetate group on ammonia nitrogen, total phosphorus and COD in the wastewater are the best, and are respectively 99.09%, 98.69%, 93.62% and 96.05%.
TABLE 3 detection results of contaminants before and after wastewater treatment under different organic carbon sources
Example 4
(1) Culturing Chlorella sorokiniana in a light incubator (25 + -1 ℃,4000 lux) by using BG11 culture solution until logarithmic phase, centrifuging and concentrating to obtain seed solution;
(2) The first stage treatment: when the initial biomass is 0.4g/L, the ratio of algae to bacteria is 1, the organic carbon source is sodium acetate and is adjusted to C/N =30, the microalgae and the activated sludge are inoculated into 500mL of actual pig-raising biogas slurry wastewater which is sterilized, the culture conditions are 6000 lux,24 h of continuous illumination, and the culture period is 7 days;
(3) And (3) second-stage treatment: after 7 days, centrifuging at high speed, collecting supernatant, performing autoclaving on the collected supernatant again to obtain culture medium for inoculating microalgae with initial biomass of 0.4g/L, and performing second-stage advanced treatment on the wastewater; the culture condition is 6000 lux,24 h continuous illumination, and the culture period is 7 days;
(4) Taking water samples every 24h to determine the biomass of the algae liquid, the TN, the TP and the NH of the wastewater 4 + The concentrations of N and COD are used for analyzing the treatment condition of pollutants in the biogas slurry wastewater and the growth condition of microorganisms; and weighing a certain amount of algae powder after the two stages are finished to measure the contents of grease, total sugar and protein accumulated by the microalgae.
The results shown in Table 4 show that the algal mud system can treat TN, TP and NH in wastewater after the two-stage treatment 4 + The removal rates of N and COD are respectively 96.70%, 96.97%, 96.95% and 81.11%, and finally can reach the discharge standard of pollutants for livestock and poultry breeding industry (GB 18596-2001); the biomass yield was 3.64 g/L and 31.16%, 25.4% were produced9% and 41.90% of oil, polysaccharide and protein biomass energy.
TABLE 4 detection results of each pollutant content before and after wastewater treatment
As can be seen from the final concentrations of the pollutants in FIG. 1, the ammonia nitrogen, total phosphorus and COD contents in the sewage treated in example 4 are 16.98mg/L, 18.72 mg/L, 0.48 mg/L and 263.49 mg/L respectively, and the sewage can reach the discharge standard.
Comparative example 1
(1) Culturing Chlorella sorokiniana in a light incubator (25 + -1 ℃,4000 lux) by using BG11 culture solution until logarithmic phase, centrifuging and concentrating to obtain seed solution;
(2) The first stage treatment: the initial biomass is 0.4g/L, the ratio of algae to bacteria is 1;
(3) And (3) second-stage treatment: after 7 days, collecting supernatant after high-speed centrifugation, performing autoclaving on the collected supernatant again to obtain a culture medium for inoculating microalgae, wherein the initial biomass of the microalgae is 0.4g/L, and continuously performing second-stage advanced treatment on the wastewater; the culture condition is 6000 lux,24 h continuous illumination, and the culture period is 7 days;
(4) Taking water samples every 24h to determine the biomass of the algae liquid, the TN, the TP and the NH of the wastewater 4 + The concentrations of N and COD are used for analyzing the treatment condition of pollutants in the biogas slurry wastewater and the growth condition of microorganisms; meanwhile, after the two stages are finished, a certain amount of algae powder is weighed to determine the contents of oil, total sugar and protein accumulated in the microalgae.
The results shown in Table 5 show that the algal mud system can treat TN, TP and NH in wastewater after the two-stage treatment 4 + The removal rates of N and COD are 91.95%, 73.22%, 83.34% and 81.25% respectively, and ammonia nitrogen can not reach the discharge standard of pollutants for livestock and poultry breeding (GB 18596-2001); the biomass accumulated 1.37 g/L,and 29.78%, 25.24% and 40.83% of the biomass energy of fats, polysaccharides and proteins is produced.
TABLE 5 detection results of each pollutant in wastewater treated in comparative example 1
The final concentration of each pollutant in the graph 2 can be obtained, and in comparative example 1, the contents of ammonia nitrogen, total phosphorus and COD in the treated sewage are respectively 92.42 mg/L, 45.28 mg/L, 4.10 mg/L and 258.34 mg/L, and the ammonia nitrogen can not reach the discharge standard.
And (4) analyzing results:
the best algae bacterium ratio for removing ammonia nitrogen and TN in the biogas slurry wastewater is 1, and the removal rates are 69.33% and 69.52% respectively. The difference in the removal rate of TP was not significant in the 5 algal inoculum ratio, and the removal rate of TP was 61.67% when the algal ratio was 1. At the end of the experiment, the COD was removed at 703.16 mg/L. As described above, when the ratio of phycomycetes =1 =3, the removal effect of nitrogen elements in the system is the best, and the growth of microorganisms is also better, and the removal effect of TP and COD in wastewater is also better. When the C/N =30, the removal rates of ammonia nitrogen, TN, TP and COD in the wastewater are the highest and are respectively 93.09%, 92.82%, 95.75% and 94.03%, and compared with the original concentration wastewater, the removal of pollutants by an algae bacterium symbiotic system is obviously improved.
After each pollution index in the biogas slurry wastewater is continuously treated in two stages of phycobiont and microalgae under the optimal C/N, the final concentration of 4 pollutants reaches the livestock and poultry breeding wastewater discharge standard. The ammonia nitrogen content of the actual wastewater is reduced from 556.85 mg/L to 16.98mg/L after being treated for 14 days, the TN content is reduced from 567.69 mg/L to 18.72 mg/L, and the removal rates are 96.95 percent and 96.70 percent respectively. After the TP is cultured for 14 days, the concentration of the phosphorus is reduced from 15.78 mg/L to 0.48 mg/L, the removal rate is as high as 96.97 percent, and the COD is finally stabilized at 263.49 mg/L. Therefore, the high-ammonia nitrogen and phosphorus removal of the livestock and poultry wastewater by using the high-efficiency two-stage continuous treatment system (phycobionics and microalgae system) can ensure that the biogas slurry wastewater reaches the discharge standard of pollutants for livestock and poultry breeding (GB 18596-2001).
Claims (9)
1. A method for removing nitrogen and phosphorus from high ammonia nitrogen livestock wastewater by a two-stage treatment system is characterized by comprising the following steps:
(1) Culturing the chlorella sofosvenorii in a light incubator by using BG11 culture solution until the culture reaches a logarithmic phase, and performing centrifugal concentration to obtain seed solution;
(2) The first stage treatment: inoculating chlorella sorokiniana solution and activated sludge into sterilized biogas slurry wastewater, adding an organic carbon source, controlling the carbon-nitrogen ratio, and culturing to obtain a treatment solution;
(3) And (3) second-stage treatment: centrifuging the first-stage treatment solution at a high speed, collecting supernatant, performing autoclaving on the collected supernatant again to obtain a culture medium for inoculating microalgae, adding chlorella sorokiniana solution, and performing second-stage advanced treatment on the wastewater to complete the treatment.
2. The method of claim 1, wherein the biomass in the seed solution of step (1) is 0.4-0.8 g/L.
3. The method according to claim 1, wherein the activated sludge in step (2) is an organic sludge, preferably an aerobic sludge.
4. The method according to claim 1, wherein the chlorella sorokiniana solution in the step (2) is mixed with the activated sludge according to a ratio of 1 (1-5) to 1 (1-5) in terms of MLSS, w/w.
5. The method according to claim 1, wherein the culture conditions in step (2) are illumination intensity of 4000-6000 lux, and the culture is carried out under 24h continuous illumination, and the Erlenmeyer flask is shaken for 1-3 min in the morning, at night and each day; the time of the deep treatment is at least 5 days; preferably the treatment time is at least 7 days.
6. The method according to claim 1, wherein the carbon source in the step (2) is at least one selected from the group consisting of glucose, sodium acetate, starch and citric acid.
7. The method of claim 1, wherein the carbon-to-nitrogen ratio in step (2) is C/N =2.2-30.
8. The method of claim 1, wherein the second stage of step (3) comprises adding chlorella sorokiniana at an initial biomass of 0.4-0.8 g/L.
9. The method according to claim 1, wherein the conditions of the intensive treatment in step (3) are that the culture is carried out under continuous illumination of 4000-6000 lux for 24 hours, and the Erlenmeyer flask is shaken for 1-3 min each day in the morning, at the evening; the time of the deep treatment is at least 5 days; preferably the treatment time is at least 7 days.
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| CN106219871A (en) * | 2016-08-09 | 2016-12-14 | 重庆大学 | A kind of livestock breeding wastewater processing method |
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