CN115400155A - Probiotic composition for relieving postpartum depression and preparation method and application thereof - Google Patents

Probiotic composition for relieving postpartum depression and preparation method and application thereof Download PDF

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CN115400155A
CN115400155A CN202210989401.9A CN202210989401A CN115400155A CN 115400155 A CN115400155 A CN 115400155A CN 202210989401 A CN202210989401 A CN 202210989401A CN 115400155 A CN115400155 A CN 115400155A
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lactobacillus
probiotic
probiotic composition
weight
weight ratio
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肖归
谢仲鹏
王静
姚欢
王虎
胡佳吉
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Central South University
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Central South University
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Abstract

The invention relates to the field of health-care food, and discloses a probiotic composition for relieving postpartum depression, a preparation method and application thereof, wherein the probiotic composition contains probiotics, prebiotics and active ingredients; the probiotic contains Lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus reuteri and Lactobacillus helveticus; the active ingredients comprise beta-cryptoxanthin and N-acetylneuraminic acid. The probiotic composition can regulate intestinal flora ecology, relieve abnormal emotion, and relieve postpartum depression.

Description

Probiotic composition for relieving postpartum depression and preparation method and application thereof
Technical Field
The invention relates to the field of health-care food, and in particular relates to a probiotic composition for relieving postpartum depression, and a preparation method and application thereof.
Background
Postpartum Depression (postspecific Depression) refers to a psychological disorder characterized by a series of symptoms of Depression, sadness, depression, crying, irritability, dysphoria, even suicidal or baby-killing tendency of a parturient after childbirth, and is one of the most common types of puerperal mental syndromes. Typical postpartum depression occurs within 6 weeks of delivery, and may persist throughout puerperium, with some continuing even before the infant is school-time. The incidence rate of postpartum depression is 15% -30%, and the recurrence rate of the second pregnancy is 20% -30%.
At present, western medicines mainly take effective antidepressants for treating postpartum depression, and the western medicines have the disadvantages of great side effects, high price and influence on postpartum lactation, so that the compliance of patients is poor. Although traditional Chinese medicine has a certain effect on treating postpartum depression, the traditional Chinese medicine also has the problems of insignificant curative effect, influence on lactation of puerperae due to long-term taking of the medicine, poor compliance of the puerperae and the like, so that further improvement is needed.
Scientific researches in recent years find that most postpartum depression patients have at least one intestinal function disease, such as diarrhea, constipation, intestinal inflammation, poor nutrition absorption function and the like, and the discomfort of the intestinal function can bring emotional disorders, such as easy anxiety, anger, poor spleen qi, depression and the like to the patients. Probiotics is a kind of active microorganisms beneficial to a host, and is a general term for active beneficial microorganisms which are planted in the intestinal tract and the reproductive system of a human body and can generate definite health efficacy so as to improve the micro-ecological balance of the host and play beneficial roles. The probiotics have important effects on the aspects of adjusting the ecology of intestinal flora and relieving abnormal emotion, at present, single probiotics or a composition of the probiotics and Chinese herbal medicines is mostly used in the market to adjust the intestinal flora, and the research on the probiotic compound formula for adjusting the ecology of the intestinal flora and relieving the abnormal emotion by the synergistic effect of various probiotics is less.
Disclosure of Invention
The invention aims to overcome the problems in the prior art and provides a probiotic composition for relieving postpartum depression, a preparation method and application thereof.
The invention provides a probiotic composition for relieving postpartum depression, which comprises probiotics, prebiotics and active ingredients; the probiotic contains Lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus reuteri and Lactobacillus helveticus; the active ingredients comprise beta-cryptoxanthin and N-acetylneuraminic acid.
Preferably, the prebiotics are contained in an amount of 1.5 to 5 parts by weight and the active ingredient is contained in an amount of 0.2 to 0.5 part by weight, relative to 10 parts by weight of the probiotic.
Preferably, the weight ratio of the lactobacillus acidophilus, the lactobacillus bulgaricus, the lactobacillus reuteri and the lactobacillus helveticus is 1-5:1-5:1-2:1.
preferably, the weight ratio of the β -cryptoxanthin to the N-acetylneuraminic acid is 1:1-4.
Preferably, the probiotic bacteria further comprise bifidobacterium longum, lactobacillus rhamnosus and bifidobacterium breve.
Preferably, the weight ratio of bifidobacterium longum, lactobacillus rhamnosus, bifidobacterium breve to lactobacillus helveticus is 0.2-1:0.5-2:0.2-1:1.
preferably, the prebiotic is selected from at least one of fructooligosaccharide, galactooligosaccharide, stachyose, inulin, isomaltooligosaccharide and trehalose.
Preferably, the prebiotic is fructooligosaccharide, galactooligosaccharide, inulin, and isomaltooligosaccharide.
Preferably, the weight ratio of said fructooligosaccharides, said galactooligosaccharides, said inulin and said isomaltooligosaccharides is 1:0.5-2:1-4:1-4.
Preferably, the probiotic composition further comprises an adjuvant.
Preferably, the content of the adjuvant is 1-2.5 parts by weight relative to 10 parts by weight of the probiotic bacteria.
Preferably, the auxiliary material is selected from at least one of yeast powder, skim milk powder, maltodextrin and sugar alcohol.
The invention provides a preparation method of a probiotic composition for relieving postpartum depression, which comprises the following steps: mixing probiotics, prebiotics and active ingredients to obtain a mixture; the probiotic contains Lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus reuteri and Lactobacillus helveticus; the active ingredients comprise beta-cryptoxanthin and N-acetylneuraminic acid.
Preferably, the prebiotics are contained in an amount of 1.5 to 5 parts by weight and the active ingredient is contained in an amount of 0.2 to 0.5 part by weight, relative to 10 parts by weight of the probiotic.
Preferably, the weight ratio of said lactobacillus acidophilus, said lactobacillus bulgaricus, said lactobacillus reuteri and said lactobacillus helveticus is 1-5:1-5:1-2:1.
preferably, the weight ratio of the β -cryptoxanthin to the N-acetylneuraminic acid is 1:1-4.
Preferably, the probiotic bacteria further comprise bifidobacterium longum, lactobacillus rhamnosus and bifidobacterium breve.
Preferably, the weight ratio of bifidobacterium longum, lactobacillus rhamnosus, bifidobacterium breve to lactobacillus helveticus is 0.2-1:0.5-2:0.2-1:1.
preferably, the prebiotic is selected from at least one of fructooligosaccharide, galactooligosaccharide, stachyose, inulin, isomaltooligosaccharide and trehalose.
Preferably, the prebiotic is fructooligosaccharide, galactooligosaccharide, inulin, and isomaltooligosaccharide.
Preferably, the weight ratio of the fructooligosaccharides, the galactooligosaccharides, the inulin and the isomaltooligosaccharides is 1:0.5-2:1-4:1-4.
Preferably, the preparation method further comprises: and mixing the mixture with auxiliary materials to obtain the probiotic composition.
Preferably, the weight ratio of the auxiliary material to the probiotics is 0.1-0.25:1.
preferably, the auxiliary material is selected from at least one of yeast powder, skim milk powder, maltodextrin and sugar alcohol.
In a third aspect, the present invention provides a use of the probiotic composition of the first aspect and/or the probiotic composition obtained by the preparation method of the second aspect in preparing a medicament and/or food for relieving postpartum depression.
Through the technical scheme, the invention has the beneficial effects that:
(1) The probiotic composition for relieving postpartum depression provided by the invention contains probiotics, prebiotics and active ingredients, and can effectively regulate intestinal flora and relieve abnormal mood through the combined action of the probiotics, the prebiotics and the active ingredients, so that the effects of improving sleep quality and relieving postpartum depression are achieved. The lactobacillus acidophilus, the lactobacillus bulgaricus, the lactobacillus reuteri and the lactobacillus helveticus have a synergistic effect, and under the combined action, a good effect of relieving postpartum depression is achieved; moreover, the beta-cryptoxanthin and the N-acetylneuraminic acid can improve the sleep effect and play a role in relieving the postpartum depression, and the beta-cryptoxanthin and the N-acetylneuraminic acid have a synergistic effect and further play a role in relieving the postpartum depression under the combined action.
(2) The probiotic composition for relieving postpartum depression provided by the invention can be added with bifidobacterium longum, lactobacillus rhamnosus and bifidobacterium breve, can cooperate with lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus reuteri and lactobacillus helveticus, can further regulate intestinal flora, relieve abnormal mood, and better play a role in improving sleep quality and relieving postpartum depression.
Detailed Description
The endpoints of the ranges and any values disclosed herein are not limited to the precise range or value, and such ranges or values should be understood to encompass values close to those ranges or values. For ranges of values, between the endpoints of each of the ranges and the individual points, and between the individual points may be combined with each other to give one or more new ranges of values, and these ranges of values should be considered as specifically disclosed herein.
The invention provides a probiotic composition for relieving postpartum depression, which contains probiotics, prebiotics and active ingredients; the probiotic bacteria comprise Lactobacillus acidophilus (Lactobacillus acidophilus), lactobacillus Bulgaricus (Lactobacillus Bulgaricus), lactobacillus reuteri (Lactobacillus reuteri) and Lactobacillus helveticus (Lactobacillus helveticus); the active ingredients comprise beta-cryptoxanthin and N-acetylneuraminic acid.
The inventor discovers in the research process that the probiotic composition contains probiotics, prebiotics and active ingredients, and can effectively regulate intestinal flora and relieve abnormal mood through the combined action of the probiotics, the prebiotics and the active ingredients, so that the effects of improving sleep quality and relieving postpartum depression are achieved. The lactobacillus acidophilus, the lactobacillus bulgaricus, the lactobacillus reuteri and the lactobacillus helveticus have a synergistic effect, and under the combined action, the good effect of relieving the postpartum depression is achieved.
According to the present invention, in order to further exert the effect of the probiotic composition on relieving postpartum depression, preferably, the content of the prebiotic is 1.5 to 5 parts by weight, specifically 1.5 parts by weight, 2 parts by weight, 2.5 parts by weight, 3 parts by weight, 3.5 parts by weight, 4 parts by weight, 4.5 parts by weight, 5 parts by weight, or any value therebetween, relative to 10 parts by weight of the probiotic; the content of the active ingredient is 0.2 to 0.5 part by weight, and specifically may be 0.2 part by weight, 0.3 part by weight, 0.4 part by weight, 0.5 part by weight, or any value therebetween.
According to the present invention, preferably, the weight ratio of said lactobacillus acidophilus, said lactobacillus bulgaricus, said lactobacillus reuteri to said lactobacillus helveticus is 1-5:1-5:1-2:1. the inventor finds that under the preferred embodiment, lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus reuteri and lactobacillus helveticus are reasonably compatible to form a relatively stable combination, and the interaction among various probiotics can effectively relieve abnormal mood under the combined action, thereby playing the roles of improving sleep quality and relieving postpartum depression.
According to the invention, preferably, the weight ratio of the β -cryptoxanthin to the N-acetylneuraminic acid is 1:1-4, specifically 1:1. 1: 2. 1: 3. 1:4, or any value in between the two values. The inventor finds that under the preferred embodiment, the beta-cryptoxanthin and the N-acetylneuraminic acid can improve the sleep effect and play a role in relieving postpartum depression; moreover, the beta-cryptoxanthin and the N-acetylneuraminic acid have a synergistic effect, and further play a role in relieving postpartum depression under the combined action.
According to the present invention, preferably, the probiotic bacteria further contain Bifidobacterium longum (Bifidobacterium longum), lactobacillus rhamnosus (Lactobacillus rhamnosus) and Bifidobacterium breve (Bifidobacterium breve). The inventor finds that in the preferred embodiment, bifidobacterium longum, lactobacillus rhamnosus and bifidobacterium breve can further cooperate with lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus reuteri and lactobacillus helveticus to better regulate intestinal flora, relieve abnormal mood, improve sleep quality and play a role in relieving postpartum depression.
According to the present invention, in order to further regulate intestinal flora and relieve abnormal emotions, the weight ratio of the bifidobacterium longum, the lactobacillus rhamnosus, the bifidobacterium breve to the lactobacillus helveticus is preferably 0.2-1:0.5-2:0.2-1:1.
in the present invention, there is no particular limitation on the source of the above probiotics, and the selected probiotic strain may be cultured according to any suitable method known in the art and the selected probiotic powder prepared for addition of the probiotic composition of the present invention by, for example, freeze-drying or spray-drying; the probiotic powder can also be obtained directly by commercial purchase.
According to the present invention, preferably, the prebiotic is selected from at least one of fructooligosaccharide, galactooligosaccharide, stachyose, inulin, isomaltooligosaccharide and trehalose. The inventor finds that in the preferred embodiment, by reasonably matching the prebiotics and the probiotics, the effects of the probiotics and the prebiotics can be exerted to the maximum extent, the growth and the propagation of the probiotics can be promoted, the intestinal flora can be effectively regulated, and abnormal emotions can be relieved.
According to the present invention, in order to further regulate intestinal flora and relieve abnormal emotions, the prebiotics are preferably fructo-oligosaccharide, galacto-oligosaccharide, inulin and isomalto-oligosaccharide.
According to the present invention, in order to further regulate intestinal flora and relieve abnormal mood, the weight ratio of the fructooligosaccharide, the galactooligosaccharide, the inulin, and the isomaltooligosaccharide is preferably 1:0.5-2:1-4:1-4.
According to the present invention, preferably, the probiotic composition further comprises an adjuvant. In this preferred embodiment, the probiotic composition may be prepared into different requirements, such as powders, granules, capsules, etc.
According to the present invention, in order to improve the mouthfeel of the probiotic composition, the content of the adjuvant is preferably 1 to 2.5 parts by weight, specifically 1 part by weight, 1.5 parts by weight, 2 parts by weight, 2.5 parts by weight, or any value therebetween, relative to 10 parts by weight of the probiotic.
According to the present invention, the kind of the auxiliary materials is not particularly limited, and can be selected conventionally in the art. Preferably, the auxiliary material is selected from at least one of yeast powder, skim milk powder, maltodextrin and sugar alcohol, and is further preferably yeast powder and skim milk powder. The inventor finds that in the preferable embodiment, the yeast powder contains antioxidant, so that the liver can be protected, a certain detoxifying effect is achieved, and minerals such as selenium and chromium in the yeast powder can resist aging, resist tumors, prevent arteriosclerosis and improve the immunity of a human body; the skim milk powder has low fat content, is easy to store, and is not easy to generate oxidation.
According to the present invention, the amount of the yeast powder and the skim milk powder is not particularly limited, and may be selected conventionally in the art, and in order to further improve the mouthfeel of the probiotic composition, it is preferable that the weight ratio of the yeast powder to the skim milk powder is 1:48-52.
The above substances are all commercially available.
The invention provides a preparation method of a probiotic composition for relieving postpartum depression, which comprises the following steps: mixing probiotics, prebiotics and active ingredients to obtain a mixture; the probiotic contains Lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus reuteri and Lactobacillus helveticus; the active ingredients comprise beta-cryptoxanthin and N-acetylneuraminic acid.
According to the invention, preferably, the prebiotics are present in an amount of 1.5 to 5 parts by weight and the active ingredient is present in an amount of 0.2 to 0.5 part by weight, relative to 10 parts by weight of the probiotic.
According to the present invention, preferably, the weight ratio of said lactobacillus acidophilus, said lactobacillus bulgaricus, said lactobacillus reuteri and said lactobacillus helveticus is 1-5:1-5:1-2:1.
according to the invention, preferably, the weight ratio of the β -cryptoxanthin to the N-acetylneuraminic acid is 1:1-4.
According to the invention, preferably, the probiotic bacteria also contain bifidobacterium longum, lactobacillus rhamnosus and bifidobacterium breve.
According to the invention, preferably, the weight ratio of bifidobacterium longum, lactobacillus rhamnosus, bifidobacterium breve to lactobacillus helveticus is between 0.2 and 1:0.5-2:0.2-1:1.
according to the present invention, preferably, the prebiotic is selected from at least one of fructooligosaccharide, galactooligosaccharide, stachyose, inulin, isomaltooligosaccharide and trehalose.
According to the present invention, preferably, the prebiotic is fructooligosaccharide, galactooligosaccharide, inulin and isomaltooligosaccharide.
According to the invention, the weight ratio of the fructooligosaccharides, the galactooligosaccharides, the inulin and the isomaltooligosaccharides is preferably 1:0.5-2:1-4:1-4.
According to the present invention, preferably, the preparation method further comprises: and mixing the mixture with auxiliary materials to obtain the probiotic composition.
According to the invention, preferably, the weight ratio of the auxiliary material to the probiotic bacteria is 0.1-0.25:1, specifically, it may be 0.1: 1. 0.15: 1. 0.2: 1. 0.25:1, or any value in between the two values.
According to the present invention, preferably, the auxiliary material is selected from at least one of yeast powder, skim milk powder, maltodextrin, and sugar alcohol.
According to the invention, the prebiotic and the adjuvant may be dried separately before being mixed. The drying may be carried out by any conventional drying means, such as hot air drying, freeze drying, etc. Preferably, the drying conditions include: the temperature is 35-45 deg.C, specifically 35 deg.C, 40 deg.C, 45 deg.C, or any value between the above two values; the time is 30-60min, specifically 30min, 40min, 50min, 60min, or any value between the above two values.
According to the present invention, the mixing method is not particularly limited, and conventional selection in the art can be adopted, and preferably, the rotation speed is 50 to 100rpm, specifically, 50rpm, 60rpm, 70rpm, 80rpm, 90rpm, 100rpm, or any value between the two values; the time is 5-10min, specifically 5min, 6min, 7min, 8min, 9min, 10min, or any value therebetween.
In the present invention, the probiotic composition is preferably dispensed and sealed after it has been obtained. The method of dispensing and sealing is not particularly limited, and a method of dispensing and sealing conventional in the art may be employed.
In a third aspect, the present invention provides a use of the probiotic composition of the first aspect and/or the probiotic composition obtained by the preparation method of the second aspect in preparing a medicament and/or food for relieving postpartum depression.
According to a particularly preferred embodiment of the present invention, there is provided a method for preparing a probiotic composition for relieving postpartum depression, comprising the steps of:
(1) Respectively drying prebiotics and adjuvants at 35-45 deg.C for 30-60min;
(2) Mixing probiotics, prebiotics, active ingredients and auxiliary materials, and stirring for 5-10min under the condition that the rotating speed is 50-100rpm to obtain the probiotic composition;
the probiotic bacteria comprise Lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus reuteri, lactobacillus helveticus, bifidobacterium longum, lactobacillus rhamnosus and Bifidobacterium breve; the active ingredients comprise beta-cryptoxanthin and N-acetylneuraminic acid;
relative to 10 parts by weight of the probiotics, the content of the prebiotics is 1.5-5 parts by weight, the content of the active ingredients is 0.2-0.5 part by weight, and the content of the auxiliary materials is 1-2.5 parts by weight; the weight ratio of the lactobacillus acidophilus, the lactobacillus bulgaricus, the lactobacillus reuteri, the bifidobacterium longum, the lactobacillus rhamnosus, the bifidobacterium breve to the lactobacillus helveticus is 1-5:1-5:1-2:0.2-1:0.5-2:0.2-1:1; the weight ratio of the beta-cryptoxanthin to the N-acetylneuraminic acid is 1:1-4; the prebiotics are fructo-oligosaccharide, galacto-oligosaccharide, inulin and isomaltooligosaccharide; the weight ratio of the fructo-oligosaccharide, the galacto-oligosaccharide, the inulin and the isomalto-oligosaccharide is 1:0.5-2:1-4:1-4; the auxiliary materials are yeast powder and skim milk powder.
In the above particularly preferred embodiment, the probiotics, prebiotics and active ingredients are reasonably matched, and under the combined action, the ecology of the intestinal flora can be adjusted, abnormal mood can be relieved, and the effect of relieving postpartum depression can be achieved; and the probiotic composition has the advantages of simple production process, convenient operation and low production cost.
The present invention will be described in detail below by way of examples.
In the following examples and comparative examples, lactobacillus acidophilus was obtained from Shannon Biotech (Suzhou) Inc. under the model AL12; lactobacillus bulgaricus was purchased from Shangen Biotech (Suzhou) Inc. under model number LB10; lactobacillus reuteri was obtained from shann kang biotechnology (suzhou) limited as model RL17; lactobacillus helveticus was purchased from shangkang biotechnology (suzhou) limited, model number HL32; bifidobacterium longum is available from Shangen Biotechnology (Suzhou) Inc. with model number LB32; lactobacillus rhamnosus was purchased from shangkang biotechnology (suzhou) ltd, model No. NJ551; bifidobacterium breve was purchased from Shangen Biotechnology (Suzhou) Inc. under model number BB28; beta-cryptoxanthin was purchased from Shanghai Allantin Biotechnology, inc. under the model number C339418; n-acetylneuraminic acid was purchased from Shanghai Michelin Biotechnology Ltd, model No. N801293; other materials were commercially available unless otherwise specified.
Example 1
(1) Respectively placing fructo-oligosaccharide, galacto-oligosaccharide, inulin, isomaltooligosaccharide, yeast powder and skimmed milk powder into an oven, and drying at 40 deg.C for 40min;
(2) Weighing 1g of fructo-oligosaccharide, 1g of galacto-oligosaccharide, 1.5g of inulin, 1.5g of isomaltooligosaccharide, 0.06g of yeast powder and 3g of skim milk powder obtained after drying in the step (1), putting into a container, and stirring for 5min under the condition that the rotating speed is 80rpm to obtain a mixture;
(3) And (3) adding 4g of lactobacillus acidophilus, 4g of lactobacillus bulgaricus, 3g of lactobacillus reuteri, 2g of lactobacillus helveticus, 1g of bifidobacterium longum, 2g of lactobacillus rhamnosus, 1g of bifidobacterium breve, 0.2g of beta-cryptoxanthin and 0.4g N-acetylneuraminic acid into the mixture obtained in the step (2), and stirring for 5min at the rotating speed of 80rpm to obtain the probiotic composition.
Example 2
(1) Respectively placing fructo-oligosaccharide, galacto-oligosaccharide, inulin, isomaltooligosaccharide, yeast powder and skimmed milk powder into an oven, and drying at 35 deg.C for 60min;
(2) Weighing 1g of fructo-oligosaccharide obtained after drying in the step (1), 0.5g of galacto-oligosaccharide, 1g of inulin, 1g of isomaltooligosaccharide, 0.1g of yeast powder and 5g of skim milk powder, putting into a container, and stirring for 5min under the condition that the rotation speed is 50rpm to obtain a mixture;
(3) And (3) adding 10g of lactobacillus acidophilus, 2g of lactobacillus bulgaricus, 4g of lactobacillus reuteri, 2g of lactobacillus helveticus, 2g of bifidobacterium longum, 1g of lactobacillus rhamnosus, 0.4g of bifidobacterium breve, 0.1g of beta-cryptoxanthin and 0.4g N-acetylneuraminic acid into the mixture obtained in the step (2), and stirring for 5min at the rotation speed of 50rpm to obtain the probiotic composition.
Example 3
(1) Respectively placing fructo-oligosaccharide, galacto-oligosaccharide, inulin, isomaltooligosaccharide, yeast powder and skimmed milk powder into an oven, and drying at 45 deg.C for 30min;
(2) Weighing 1g of fructo-oligosaccharide, 2g of galacto-oligosaccharide, 4g of inulin, 4g of isomaltooligosaccharide, 0.05g of yeast powder and 2.5g of skim milk powder obtained after drying in the step (1), putting into a container, and stirring for 5min under the condition that the rotation speed is 100rpm to obtain a mixture;
(3) And (3) adding 2g of lactobacillus acidophilus, 10g of lactobacillus bulgaricus, 2g of lactobacillus reuteri, 2g of lactobacillus helveticus, 0.4g of bifidobacterium longum, 4g of lactobacillus rhamnosus, 2g of bifidobacterium breve, 0.5g of beta-cryptoxanthin and 0.5g N-acetylneuraminic acid into the mixture obtained in the step (2), and stirring for 5min at the rotating speed of 100rpm to obtain the probiotic composition.
Example 4
A probiotic composition was prepared according to the method of example 1, except that the amount of N-acetylneuraminic acid was replaced with 2g.
Example 5
A probiotic composition was prepared according to the method of example 1, except that the amount of lactobacillus acidophilus was replaced with 1g, the amount of lactobacillus bulgaricus was replaced with 1g, and the amount of lactobacillus reuteri was replaced with 9g.
Example 6
(1) Placing galactooligosaccharide into oven, and drying at 40 deg.C for 40min;
(2) Mixing 5g of galactooligosaccharide obtained after drying in the step (1) with 4g of lactobacillus acidophilus, 4g of lactobacillus bulgaricus, 3g of lactobacillus reuteri, 2g of lactobacillus helveticus, 1g of bifidobacterium longum, 2g of lactobacillus rhamnosus, 1g of bifidobacterium breve, 0.2g of beta-cryptoxanthin and 0.4g N-acetylneuraminic acid, and stirring for 5min under the condition of the rotation speed of 50rpm to obtain the probiotic composition.
Example 7
A probiotic composition was prepared according to the method of example 1, except that step (3) was replaced by:
(3) And (3) adding 4g of lactobacillus acidophilus, 4g of lactobacillus bulgaricus, 3g of lactobacillus reuteri, 2g of lactobacillus helveticus, 0.2g of beta-cryptoxanthin and 0.4g N-acetylneuraminic acid into the mixture obtained in the step (2), and stirring for 5min at the rotation speed of 50rpm to obtain the probiotic composition.
Example 8
A probiotic composition was prepared according to the method of example 1, except that step (2) was replaced by:
(2) Weighing 1g of fructooligosaccharide obtained after drying in the step (1), 1g of galactooligosaccharide, 1.5g of inulin and 1.5g of isomaltooligosaccharide, putting into a container, and stirring for 5min at the rotation speed of 50rpm to obtain a mixture.
Example 9
A probiotic composition was prepared according to the method of example 1, except that the amount of β -cryptoxanthin was replaced with 0.05g and the amount of n-acetylneuraminic acid was replaced with 0.1g.
Comparative example 1
A probiotic composition was prepared according to the method of example 1, except that step (3) was replaced by:
(3) And (3) adding 4g of lactobacillus acidophilus, 4g of lactobacillus bulgaricus, 3g of lactobacillus reuteri, 2g of lactobacillus helveticus, 1g of bifidobacterium longum, 2g of lactobacillus rhamnosus and 1g of bifidobacterium breve into the mixture obtained in the step (2), and stirring for 5min at the rotation speed of 50rpm to obtain the probiotic composition.
Comparative example 2
A probiotic composition was prepared according to the method of example 1, except that step (3) was replaced by:
(3) And (3) adding 4g of lactobacillus acidophilus, 4g of lactobacillus bulgaricus, 3g of lactobacillus reuteri, 2g of lactobacillus helveticus, 1g of bifidobacterium longum, 2g of lactobacillus rhamnosus, 1g of bifidobacterium breve and 0.2g of beta-cryptoxanthin into the mixture obtained in the step (2), and stirring for 5min at the rotation speed of 50rpm to obtain the probiotic composition.
Comparative example 3
A probiotic composition was prepared according to the method of example 1, except that step (3) was replaced by:
(3) And (3) adding 4g of lactobacillus bulgaricus, 3g of lactobacillus reuteri, 2g of lactobacillus helveticus, 1g of bifidobacterium longum, 2g of lactobacillus rhamnosus, 1g of bifidobacterium breve, 0.2g of beta-cryptoxanthin and 0.4g N-acetylneuraminic acid into the mixture obtained in the step (2), and stirring for 5min at the rotating speed of 50rpm to obtain the probiotic composition.
Test example
The mouse tail suspension test and the forced swimming test are routine animal experiments for verifying whether the drug has obvious functions of relieving anxiety and depression after administration. ICR mice, 18-22g in each male and female half, after 1 week of adaptive feeding, were randomly divided into 13 groups, 6 mice in each group were fed with the probiotic compositions prepared in examples 1-9 and comparative examples 1-3, respectively, at an amount of 0.1g/kg, the drug was formulated into a suspension with physiological saline and administered by intragastric administration, and the blank control group was given an equal volume of physiological saline during administration. The medicine is administered for 7 days by continuous gavage 1 time a day, and the tail suspension experiment and forced swimming experiment of mice are carried out after the last administration for 1 h.
(1) Mouse tail suspension test: mainly make its head hang down through fixed mouse afterbody, the mouse is in the panic and the struggle to run away in this environment, the state that can't escape again, provides an unavoidable oppression environment for the mouse, after a period of time, the immobility time of the mouse production desperate state process that the record is in under this environment to observe the treatment after dosing. Adhering the tail end of a mouse to a support on the upper part of a tail suspension box, turning the tail suspension box into an inverted suspension state, carrying out a tail suspension experiment, suspending 6 mice at one time, separating the heads of the inverted suspension mice by using a partition plate so as to avoid collision, struggling the mice after the inverted suspension due to panic, suspending the mice for 10 minutes when the mice are in a disappointed state after struggling fatigue and carrying out discontinuous immobility time after statistics for 5 minutes, wherein the results are shown in a table 1;
(2) Forced swimming experiment: the experimental mouse is mainly placed in limited water, the mouse struggles and struggles in the environment to try to escape and cannot escape, a non-avoidable compression environment is provided, after a period of experiment, the mouse shows the immobility state of a typical behavior periscopical state, the immobility time when the mouse generates the periscopical immobility state is recorded, the treatment effect after medication is observed, the mouse is placed in a plastic swimming box with the length of 50cm, the width of 30cm and the height of 20cm to perform a forced swimming test, the time is counted for 10 minutes after the mouse is placed in the water, and the immobility time of the mouse within 5 minutes is recorded (the mouse stops struggling or shows a floating state in the water, and only small limb movement is used for keeping the head floating on the water surface), and the result is shown in table 1.
TABLE 1
Example numbering Suspension tail rest time(s) Swimming immobility time(s)
Example 1 63.8±1.2 85.5±1.8
Example 2 64.1±2.2 85.9±2.1
Practice ofExample 3 63.9±3.1 86.3±1.6
Example 4 66.5±2.4 88.1±2.6
Example 5 68.4±1.6 88.9±1.2
Example 6 66.8±1.7 89.5±2.4
Example 7 68.7±3.3 91.2±1.8
Example 8 65.2±2.1 87.1±3.4
Example 9 70.6±1.6 92.5±1.8
Comparative example 1 75.1±1.5 95.1±1.5
Comparative example 2 72.3±1.8 93.6±2.3
Comparative example 3 73.6±2.4 94.7±1.8
Blank control group 90.4±1.7 101.9±2.3
As can be seen from the results in table 1, the probiotic compositions prepared in examples 1 to 9 of the present invention can significantly reduce the immobility time in mice of animal models with behavioral despair, compared to comparative examples 1 to 3, and thus it can be shown that the probiotic compositions prepared in the present invention have significant antidepressant effect.
The preferred embodiments of the present invention have been described above in detail, but the present invention is not limited thereto. Within the scope of the technical idea of the invention, many simple modifications can be made to the technical solution of the invention, including combinations of various technical features in any other suitable way, and these simple modifications and combinations should also be regarded as the disclosure of the invention, and all fall within the scope of the invention.

Claims (10)

1. A probiotic composition for relieving postpartum depression, wherein the probiotic composition contains probiotics, prebiotics and active ingredients;
the probiotic contains Lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus reuteri and Lactobacillus helveticus; the active ingredients comprise beta-cryptoxanthin and N-acetylneuraminic acid.
2. The probiotic composition according to claim 1, characterized in that the prebiotic is present in an amount of 1.5 to 5 parts by weight and the active ingredient is present in an amount of 0.2 to 0.5 parts by weight, relative to 10 parts by weight of the probiotic.
3. The probiotic composition according to claim 2, characterized in that the weight ratio of said lactobacillus acidophilus, said lactobacillus bulgaricus, said lactobacillus reuteri and said lactobacillus helveticus is between 1 and 5:1-5:1-2:1;
preferably, the weight ratio of the β -cryptoxanthin to the N-acetylneuraminic acid is 1:1-4.
4. The probiotic composition according to any one of claims 1 to 3, characterized in that the probiotic also contains Bifidobacterium longum, lactobacillus rhamnosus and Bifidobacterium breve;
preferably, the weight ratio of bifidobacterium longum, lactobacillus rhamnosus, bifidobacterium breve to lactobacillus helveticus is 0.2-1:0.5-2:0.2-1:1.
5. the probiotic composition according to any one of claims 1 to 3, characterized in that the prebiotic is selected from at least one of fructooligosaccharides, galactooligosaccharides, stachyose, inulin, isomaltooligosaccharides and trehalose;
preferably, the prebiotic is fructooligosaccharide, galactooligosaccharide, inulin, and isomaltooligosaccharide;
preferably, the weight ratio of the fructooligosaccharides, the galactooligosaccharides, the inulin and the isomaltooligosaccharides is 1:0.5-2:1-4:1-4.
6. The probiotic composition according to any one of claims 1 to 3, characterized in that it further comprises adjuvants;
preferably, the content of the auxiliary material is 1-2.5 parts by weight relative to 10 parts by weight of the probiotics;
preferably, the auxiliary material is selected from at least one of yeast powder, skim milk powder, maltodextrin and sugar alcohol.
7. A preparation method of a probiotic composition for relieving postpartum depression is characterized by comprising the following steps: mixing probiotics, prebiotics and active ingredients to obtain a mixture;
the probiotic bacteria comprise Lactobacillus acidophilus, lactobacillus bulgaricus, lactobacillus reuteri and Lactobacillus helveticus; the active ingredients contain beta-cryptoxanthin and N-acetylneuraminic acid.
8. The method according to claim 7, wherein the prebiotic is contained in an amount of 1.5 to 5 parts by weight and the active ingredient is contained in an amount of 0.2 to 0.5 part by weight, relative to 10 parts by weight of the probiotic bacteria;
preferably, the weight ratio of said lactobacillus acidophilus, said lactobacillus bulgaricus, said lactobacillus reuteri and said lactobacillus helveticus is 1-5:1-5:1-2:1;
preferably, the weight ratio of the β -cryptoxanthin to the N-acetylneuraminic acid is 1:1-4;
preferably, the probiotic bacteria further comprise bifidobacterium longum, lactobacillus rhamnosus and bifidobacterium breve;
preferably, the weight ratio of bifidobacterium longum, lactobacillus rhamnosus, bifidobacterium breve to lactobacillus helveticus is 0.2-1:0.5-2:0.2-1:1;
preferably, the prebiotic is selected from at least one of fructooligosaccharide, galactooligosaccharide, stachyose, inulin, isomaltooligosaccharide and trehalose;
preferably, the prebiotic is fructooligosaccharide, galactooligosaccharide, inulin, and isomaltooligosaccharide;
preferably, the weight ratio of the fructooligosaccharides, the galactooligosaccharides, the inulin and the isomaltooligosaccharides is 1:0.5-2:1-4:1-4.
9. The production method according to claim 7 or 8, characterized by further comprising: mixing the mixture with an auxiliary material to obtain the probiotic composition;
preferably, the weight ratio of the auxiliary material to the probiotics is 0.1-0.25:1;
preferably, the auxiliary material is selected from at least one of yeast powder, skim milk powder, maltodextrin and sugar alcohol.
10. Use of a probiotic composition according to any one of claims 1 to 6 and/or obtained by a method according to any one of claims 7 to 9, for the preparation of a medicament and/or a food product for alleviating postpartum depression.
CN202210989401.9A 2022-08-17 2022-08-17 Probiotic composition for relieving postpartum depression and preparation method and application thereof Pending CN115400155A (en)

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* Cited by examiner, † Cited by third party
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US20180147221A1 (en) * 2015-04-23 2018-05-31 Kaleido Biosciences, Inc. Glycan therapeutic compositions and related methods thereof
CN110913879A (en) * 2017-06-02 2020-03-24 奥塔哥大学 Use of lactic acid bacteria for treating or preventing at least one of postpartum depression and postpartum anxiety
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