CN115381924B

CN115381924B - Composition for nerve repair and preparation method thereof

Info

Publication number: CN115381924B
Application number: CN202211030646.5A
Authority: CN
Inventors: 李晴; 银孟卓
Original assignee: Guangzhou First Peoples Hospital
Current assignee: Guangzhou First Peoples Hospital
Priority date: 2022-08-26
Filing date: 2022-08-26
Publication date: 2023-09-05
Anticipated expiration: 2042-08-26
Also published as: CN115381924A

Abstract

The invention provides a composition for nerve repair, which is characterized by comprising the following components of brain protein hydrolysate, rehmannia root extract, sea cucumber extract, gastrodia elata extract, schisandra chinensis extract powder, licorice extract powder and nutrition aid, wherein the mass ratio of the brain protein hydrolysate to the rehmannia root extract to the licorice extract is 1-3:2-4:1-4:0.5-1:1-2:1-3:3-6, the active component in the rehmannia root extract comprises catalpol with the mass ratio of more than 70%, the active component in the sea cucumber extract comprises sea cucumber cerebroside with the mass ratio of more than 80% and sea cucumber phospholipid, and the active component in the gastrodia elata extract comprises gastrodin, vanillin and gastrodia elata polysaccharide with the mass ratio of more than 80%; the nutritional aid comprises compound vitamins, compound microelements, compound probiotics and compound proteins. The invention improves the stability of the nervous system from various aspects, protects the nervous system from damage and promotes the repair of the nervous system damage, and has good effect.

Description

Composition for nerve repair and preparation method thereof

Technical Field

The invention relates to the technical field of pharmacy, in particular to a composition for nerve repair and a preparation method thereof.

Background

The nervous system includes the central nervous system (brain, spinal cord) and the peripheral nervous system. Damage to the nervous system can be caused by a variety of factors: (1) Physical injury, which directly causes damage to nerve tissue at the injury site, such as brain nerve tissue injury or spinal cord injury caused by trauma; (2) Temporary or permanent ischemia or hypoxia of a part of the nervous system, such as cerebral nerve tissue damage caused by stroke or cerebral embolism; (3) Contact with neurotoxins, such as chemicals for the treatment of cancer or dideoxy cyto for the treatment of AIDS; (4) Chronic metabolic diseases such as peripheral nerve injury caused by diabetes or renal dysfunction; (5) Neurodegenerative diseases including Parkinson's disease, alzheimer's disease, and the like. And the social hidden trouble caused by aging-related diseases is increasing along with the acceleration of population aging. The aging-related nerve injury diseases are nerve cell deficiency and nerve tissue injury diseases caused by various reasons, commonly comprise vascular dementia (VaD) and most Neurodegenerative Diseases (ND), such as Alzheimer's Disease (AD), parkinson's Disease (PD) and the like, are frequently seen by old people, are mainly related to age, and the aging-related nerve injury diseases have the characteristics of high disability rate and mortality rate, seriously affect the later-year life of patients, cause larger family burden and social stress, and become an urgent task in front of domestic and overseas scientific researchers.

Many senile nerve injury diseases are closely related to aging, but the pathogenesis is not the same, and in the case of Alzheimer's disease which is one of main types, the pathogenesis of the senile nerve injury diseases is still unknown, so far, various hypotheses mainly exist about the pathogenesis of the senile nerve injury diseases, namely cholinergic hypothesis, abeta protein generation and metabolism disorder hypothesis, oxidative stress and free radical injury hypothesis, inflammation hypothesis, tau protein abnormal phosphorylation hypothesis, metal ion metabolism hypothesis and the like, however, the pathological characteristics of the senile nerve injury diseases can be completely explained without any single hypothesis, the senile nerve injury diseases are mainly considered to be the result of the combined action of various factors, and the aging is the only determinable factor. At present, no definite diagnosis and treatment methods and treatment medicines for preventing, early diagnosing and treating Alzheimer's disease exist. Accordingly, there is a need to provide a composition for nerve repair that is beneficial for nerve damage repair caused by physical, pharmaceutical, disease or aging.

Disclosure of Invention

The invention aims to provide a composition for nerve repair, which can be applied to nerve injury repair caused by physics, medicines, diseases or aging.

In order to achieve the aim, the invention provides a composition for nerve repair, which comprises the following components of brain protein hydrolysate, rehmannia root extract, sea cucumber extract, gastrodia elata extract, schisandra chinensis extract powder, licorice extract powder and nutrition aid, wherein the mass ratio of the brain protein hydrolysate to the rehmannia root extract to the sea cucumber extract is 1-3:2-4:1-4:0.5-1:1-2:1-3:3-6, the active component of the rehmannia root extract comprises catalpol with the mass ratio of more than 70%, the active component of the sea cucumber extract comprises sea cucumber cerebroside and sea cucumber phospholipid with the mass ratio of more than 80%, and the active component of the gastrodia elata extract comprises gastrodin, vanillin and gastrodia elata polysaccharide with the mass ratio of more than 80%.

The nutritional aid comprises compound vitamins, compound microelements, compound probiotics and compound proteins.

Further preferably, the mass ratio of the brain protein hydrolysate, the rehmannia root extract, the sea cucumber extract, the gastrodia elata extract, the schisandra chinensis extract powder, the liquorice extract powder and the nutritional aid is 1.8:2.5:3:0.6:1.4:2:5.5.

Further preferably, the nutritional aid comprises a mass ratio of 1.5:0.3:1:1.2, compound vitamins, compound microelements, compound probiotics and compound proteins.

Further preferred, the complex probiotics are one or more of lactobacillus, lactobacillus reuteri, clostridium butyricum, bacillus subtilis, bacillus coagulans, streptococcus thermophilus and bifidobacterium.

Further preferably, the vitamin complex is composed of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E.

Further preferably, the compound trace elements include a plurality of iron, zinc, chromium, iodine, molybdenum, selenium, and copper.

Further preferably, the method is applied to nerve injury repair caused by physics, medicine, diseases or aging. Is particularly suitable for repairing degenerative nerve injury.

The invention also provides a preparation method of the composition for nerve repair, which comprises the following steps:

s1, respectively mixing to prepare powdery compound vitamins, compound microelements, compound probiotics and compound proteins;

s2, dissolving the brain protein hydrolysate raw material medicine in water, filtering by a nanofiltration membrane, and freeze-drying the filtrate to obtain brain protein hydrolysate;

s3, mixing and uniformly stirring the compound vitamins, the compound trace elements, the coated compound probiotics, the brain protein hydrolysate, the powdery rehmannia root extract, the sea cucumber extract, the gastrodia elata extract, the shizandra berry extract powder and the licorice extract powder according to the mass ratio to obtain the composition.

Further preferably, the preparation method further comprises S4, performing primary coating on the composition by hydroxypropyl methyl cellulose phthalate, and performing low-temperature secondary coating by adopting soluble soybean polysaccharide, wherein the coating temperature is 50+/-5 ℃, and the coating time is 3 hours, so as to obtain the coating composition.

The beneficial effects of the invention are as follows: the composition for nerve repair of the invention is prepared by synergetically matching brain protein hydrolysate, rehmannia root extract, sea cucumber extract, gastrodia elata extract, schisandra chinensis extract powder, licorice extract powder and nutritional aid, and inhibits apoptosis of neurons, eliminates apoptosis of Abeta deposition of PC12 cells caused by Abeta injury and a signal mechanism; 2. oxidation, slowing down neuronal aging; 3. increasing the expression of brain-derived nerve factor and vascular endothelial growth factor to repair ischemic brain tissue; 4. regulating neurotransmitter levels in the hippocampus to protect the central nervous system; 5. promoting the survival and differentiation of neurons, improving the stability of the central nervous system, and promoting the protection and repair of the nervous system; 6. improving intestinal flora, regulating central gamma-aminobutyric acid receptor by vagus nerve to influence brain nerve chemistry (neurotransmitter level, receptor and various neurotrophic factors) and behavior, improving nerve injury or apoptosis, and promoting nerve repair; 7. promoting nutrition absorption, ensuring organism nutrition level, improving organism immunity, promoting organism repair, etc., improving nervous system stability, protecting nervous system from injury, and promoting nervous system injury repair.

On one hand, the compound vitamins, the compound microelements, the compound probiotics and the compound proteins are matched in a synergistic way, so that the organism is fully supplemented with needed nutrition respectively; secondly, the composition can inhibit the growth of harmful bacteria, promote the reproduction of beneficial bacteria, regulate the ecological balance of intestinal tracts, form a biological protection barrier, improve the immunity of organisms, improve the circulation of the intestinal tracts, strengthen the functions of the intestines and the stomach, and improve the conversion rate of substances, thereby promoting the absorption of nutrient elements by organisms, ensuring the nutrition level of the organisms and improving the immunity of the organisms.

Detailed Description

The embodiments described below are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

The invention provides a composition for nerve repair, which comprises the following components of brain protein hydrolysate, rehmannia root extract, sea cucumber extract, gastrodia elata extract, schisandra chinensis extract powder, licorice extract powder and nutritional aid, wherein the mass ratio of the brain protein hydrolysate to the rehmannia root extract to the sea cucumber extract is 1-3:2-4:1-4:0.5-1:1-2:1-3:3-6, the active component in the rehmannia root extract comprises catalpol with the mass ratio of more than 70%, the active component in the sea cucumber extract comprises sea cucumber cerebroside and sea cucumber phospholipid with the mass ratio of more than 80%, and the active component in the gastrodia elata extract comprises gastrodin, vanillin and gastrodia elata polysaccharide with the mass ratio of more than 80%; the nutritional aid comprises compound vitamins, compound microelements, compound probiotics and compound proteins.

The composition comprises brain protein hydrolysate, rehmannia root extract, sea cucumber extract, gastrodia elata extract, schisandra chinensis extract powder, licorice extract powder and nutritional aid in a mass ratio of 1.8:2.5:3:0.6:1.4:2:5.5.

The nutrition aid comprises the following components in percentage by mass of 1.5:0.3:1:1.2, compound vitamins, compound microelements, compound probiotics and compound proteins.

The compound vitamin consists of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E.

The composite protein comprises two or more of soybean protein, casein, whey protein and pea protein.

The compound probiotics are one or a mixture of more of lactobacillus, lactobacillus reuteri, clostridium butyricum, bacillus subtilis, bacillus coagulans, streptococcus thermophilus and bifidobacterium. The probiotics can resist bacterial and virus infection, promote immunity, and remove harmful bacteria injury to human body by inhibiting harmful bacteria growth, inhibiting harmful bacteria to produce toxins, and removing toxins produced by harmful bacteria. And vitamins including pantothenic acid, nicotinic acid, B1, B2, B6, vitamin K, etc. can be produced, and short chain fatty acid, antioxidant, amino acid, etc. can be produced, and has important effects on bone growth and heart health, and can prevent and treat some diseases such as intestinal syndrome, gastric ulcer, etc., regulate intestinal flora balance, improve intestinal function, and promote nutrient absorption. The active compound can be absorbed and utilized by human body after being absorbed by intestinal canal after being interacted with intestinal flora, so that the physiological activity of the active compound is reflected, nerve injury is inhibited or repaired, the beneficial intestinal flora can improve the intestinal canal function and promote the absorption of active substances, and the effectiveness and the utilization rate of the substances are improved. Meanwhile, intestinal flora can influence brain functions through an intestinal nervous system, an endocrine system, a metabolic system and an immune system. Hormones and neurotransmitters (e.g., 5-tryptamine, dopamine, gamma-aminobutyric acid, norepinephrine, etc.) produced by enteric bacteria are transmitted into the central nervous system via the vagus nerve, resulting in corresponding neuronal inhibition or excitation. Gamma-aminobutyric acid, in turn, is the major central nervous system inhibitory neurotransmitter and is involved in regulating many physiological and psychological processes. Changes in the expression of the central gamma-aminobutyric acid receptor involve pathogenesis of AD (alzheimer's disease), and lactic acid bacteria and lactobacillus reuteri in the intestinal tract can regulate the central gamma-aminobutyric acid receptor through the vagus nerve to further influence brain nerve chemistry (neurotransmitter level, receptor thereof and various neurotrophic factors) and behavior, thereby exerting the efficacy of improving AD.

The compound vitamin consists of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E. Vitamin B1 can promote appetite, strengthen the resistance of intestines and stomach, regulate metabolism of organism and maintain normal nerve activity. Vitamin C is helpful for maintaining skin and mucous membrane health, maintaining bone and gum health, promoting iron absorption, resisting oxidation, enhancing immunity, and promoting substance absorption. Vitamin D has the main physiological functions of regulating calcium and phosphorus metabolism, and especially promoting the absorption of calcium and phosphorus by small intestine; regulating excretion of calcium and phosphorus by kidney; controlling the storage of calcium and phosphorus in bones and the concentration of calcium and phosphorus in blood, etc. When vitamin D is insufficient, even if calcium and phosphorus are sufficient, the human body cannot well utilize the vitamin D. Vitamin B12 can promote protein biosynthesis, has the effects of protecting folic acid from being transferred and stored in cells, enhancing organism immunity, protecting nerves, improving organism physique and promoting nerve repair, and when vitamin B12 is deficient, researches on the animal pernicious anemia can be caused, and the situation that bone loss is accelerated usually occurs for people with lower vitamin B12 concentration in blood is shown, because vitamin B12 can remove homocysteine in blood, osteoporosis and even hip fracture caused by the hypercysteine can be prevented, and the bone is protected. Vitamin B12 can promote erythrocyte development and maturation, make the hematopoiesis function of organism in normal state, and also can maintain nervous system health, and vitamin B12 contains mineral copper, and can promote absorption of iron by gastrointestinal tract. The main active component of vitamin E in human body is alpha-tocopherol, which is an important fat-soluble antioxidant nutrient in human body, and the deficiency of vitamin E can cause the damage of neuron axonal membrane, cause the degeneration of peripheral nerve, spinal ganglion, spinal cord back root and post axis, and lead the patients to gradually appear tendon reflex weakening or disappearance, cerebellar ataxia, skin sensory disorder, abnormal body position and vibration sense, night blindness and the like.

The compound microelements comprise a plurality of iron, zinc, chromium, iodine, molybdenum, selenium and copper. Trace elements are not contained in a large amount in a human body, but are closely related to survival and health of the human body, and play a vital role in the life of the human body. Their excessive intake, deficiency, imbalance or lack may cause physiological abnormalities or diseases in the human body to varying degrees. Zinc can promote the synthesis of protein and nucleic acid in brain, promote brain development, improve the activity of zinc-containing enzymes in brain, regulate synthesis of hormone and neurotransmitter, thereby affecting nerve signal transmission, maintaining the function of hippocampus in brain, regulating synaptic transmission, and affecting learning and memory. Iodine can affect neural development by affecting thyroxine synthesis, and iodine deficiency can result in a decrease in total brain tissue weight, affecting hippocampal and motor development. The selenium element has the effects of scavenging free radicals, protecting cell membrane, regulating immunity, relieving free radical damage to brain nerve cells, and promoting repair of brain nerve injury. Whereas copper deficiency can lead to neurotransmitter transmission dysfunction, resulting in neurological dysfunction.

The damage to nervous system, especially the degenerative diseases of nervous system, is mainly caused by the elderly, which usually has gastrointestinal degeneration, intestinal mucosa atrophy, influence on nutrition absorption, and is easy to have malnutrition, especially protein intake in food is reduced, and brain function is damaged and disease condition is more serious.

The composition can be applied to nerve injury repair caused by physics, medicine, diseases or aging, and is particularly suitable for degenerative nerve injury repair.

The preparation method of the composition for nerve repair comprises the following steps:

Example 1

The embodiment provides a composition for nerve repair, which comprises the following components of a brain protein hydrolysate, a rehmannia root extract, a sea cucumber extract, a gastrodia elata extract, schisandra chinensis extract powder, licorice extract powder and nutritional aids in a mass ratio of 1:2:1:0.5:1:1:3, wherein the active component in the rehmannia root extract comprises catalpol in more than 70% by mass ratio, the active component in the sea cucumber extract comprises sea cucumber cerebroside and sea cucumber phospholipid in more than 80% by mass ratio, and the active component in the gastrodia elata extract comprises gastrodin, vanillin and gastrodia elata polysaccharide in more than 80% by mass ratio; the nutrition aid comprises the following components in percentage by mass of 1.5:0.3:1:1.2 composite vitamins, composite microelements, composite probiotics and composite proteins.

The compound vitamin consists of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E with equal mass. The composite protein comprises equal amounts of soy protein and casein. The compound probiotics comprise lactobacillus, lactobacillus reuteri, clostridium butyricum and bacillus subtilis with equal mass.

s1, respectively mixing the formula amounts of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E to prepare the compound vitamin; mixing iron fumarate, biological protein zinc, chromium picolinate, iodized salt, ammonium molybdate, malt selenium and copper sulfate to obtain a compound trace element; the lactobacillus, the lactobacillus reuteri, the clostridium butyricum and the bacillus subtilis freeze-dried powder are mixed to prepare composite probiotics; mixing soybean protein and casein powder to obtain composite protein;

Example 2

The embodiment provides a composition for nerve repair, which comprises the following components of a brain protein hydrolysate, a rehmannia root extract, a sea cucumber extract, a gastrodia elata extract, schisandra chinensis extract powder, licorice extract powder and a nutrition aid in a mass ratio of 1.4:3.5:2:0.8:1.2:2.5:4, wherein the active component in the rehmannia root extract comprises catalpol with more than 70% of the mass ratio, the active component in the sea cucumber extract comprises sea cucumber cerebroside with more than 80% of the mass ratio and sea cucumber phospholipid, and the active component in the gastrodia elata extract comprises gastrodin, vanillin and gastrodia elata polysaccharide with more than 80% of the mass ratio; the nutrition aid comprises the following components in percentage by mass of 1.5:0.3:1:1.2 composite vitamins, composite microelements, composite probiotics and composite proteins.

The compound vitamin consists of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E with equal mass. The composite protein comprises soybean protein, whey protein and pea protein with equal quality. The compound probiotics comprise lactobacillus, lactobacillus reuteri, bacillus subtilis and bacillus coagulans with equal mass.

s1, respectively mixing the formula amounts of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E to prepare the compound vitamin; mixing iron fumarate, biological protein zinc, chromium picolinate, iodized salt, ammonium molybdate, malt selenium and copper sulfate to obtain a compound trace element; the lactobacillus, the lactobacillus reuteri, the bacillus subtilis and the bacillus coagulans are mixed to prepare composite probiotics; mixing soybean protein, whey protein and pea protein powder to obtain composite protein;

Example 3

The embodiment provides a composition for nerve repair, which comprises the following components of a brain protein hydrolysate, a rehmannia root extract, a sea cucumber extract, a gastrodia elata extract, schisandra chinensis extract powder, licorice extract powder and a nutrition aid in a mass ratio of 1.8:2.5:3:0.6:1.4:2:5.5, wherein the active component in the rehmannia root extract comprises catalpol with more than 70% of the mass ratio, the active component in the sea cucumber extract comprises sea cucumber cerebroside with more than 80% of the mass ratio and sea cucumber phospholipid, and the active component in the gastrodia elata extract comprises gastrodin, vanillin and gastrodia elata polysaccharide with more than 80% of the mass ratio; the nutrition aid comprises the following components in percentage by mass of 1.5:0.3:1:1.2 composite vitamins, composite microelements, composite probiotics and composite proteins.

The compound vitamin consists of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E with equal mass. The composite protein comprises whey protein and pea protein with equal quality. The compound probiotics comprise lactobacillus, lactobacillus reuteri, clostridium butyricum, bacillus subtilis and bifidobacterium with equal mass.

s1, respectively mixing the formula amounts of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E to prepare the compound vitamin; mixing iron fumarate, biological protein zinc, chromium picolinate, iodized salt, ammonium molybdate, malt selenium and copper sulfate to obtain a compound trace element; mixing lactobacillus, lactobacillus reuteri, clostridium butyricum, bacillus subtilis and bifidobacterium freeze-dried powder to prepare composite probiotics; mixing whey protein and pea protein powder to obtain composite protein;

Example 4

The embodiment provides a composition for nerve repair, which comprises the following components of a brain protein hydrolysate, a rehmannia root extract, a sea cucumber extract, a gastrodia elata extract, schisandra chinensis extract powder, licorice extract powder and a nutrition aid in a mass ratio of 2.2:3:3.5:0.9:1.6:3:5, wherein the active component in the rehmannia root extract comprises catalpol with a mass ratio of more than 70%, the active component in the sea cucumber extract comprises sea cucumber cerebroside and sea cucumber phospholipid with a mass ratio of more than 80%, and the active component in the gastrodia elata extract comprises gastrodin, vanillin and gastrodia elata polysaccharide with a mass ratio of more than 80%; the nutrition aid comprises the following components in percentage by mass of 1.5:0.3:1:1.2 composite vitamins, composite microelements, composite probiotics and composite proteins.

The compound vitamin consists of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E with equal mass. The composite protein comprises soybean protein, casein, whey protein and pea protein with equal quality. The compound probiotics comprise lactobacillus, lactobacillus reuteri, clostridium butyricum, bacillus subtilis, bacillus coagulans, streptococcus thermophilus and bifidobacterium with equal mass.

s1, respectively mixing the formula amounts of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E to prepare the compound vitamin; mixing iron fumarate, biological protein zinc, chromium picolinate, iodized salt, ammonium molybdate, malt selenium and copper sulfate to obtain a compound trace element; lactobacillus, lactobacillus reuteri, clostridium butyricum, bacillus subtilis, bacillus coagulans, streptococcus thermophilus and bifidobacterium freeze-dried powder are mixed to prepare composite probiotics; mixing soybean protein, casein, whey protein and pea protein powder to obtain composite protein;

Example 5

The embodiment provides a composition for nerve repair, which comprises the following components of a brain protein hydrolysate, a rehmannia root extract, a sea cucumber extract, a gastrodia elata extract, schisandra chinensis extract powder, licorice extract powder and a nutrition aid in a mass ratio of 2.6:4:4:0.7:1.8:1.5:3.5, wherein the active component in the rehmannia root extract comprises catalpol with more than 70% of the mass ratio, the active component in the sea cucumber extract comprises sea cucumber cerebroside with more than 80% of the mass ratio and sea cucumber phospholipid, and the active component in the gastrodia elata extract comprises gastrodin, vanillin and gastrodia elata polysaccharide with more than 80% of the mass ratio; the nutrition aid comprises the following components in percentage by mass of 1.5:0.3:1:1.2 composite vitamins, composite microelements, composite probiotics and composite proteins.

The compound vitamin consists of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E with equal mass. The composite protein comprises soybean protein, casein and whey protein with equal quality. The compound probiotics comprise clostridium butyricum, bacillus subtilis, bacillus coagulans, streptococcus thermophilus and bifidobacterium with equal mass.

s1, respectively mixing the formula amounts of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E to prepare the compound vitamin; mixing iron fumarate, biological protein zinc, chromium picolinate, iodized salt, ammonium molybdate, malt selenium and copper sulfate to obtain a compound trace element; mixing clostridium butyricum, bacillus subtilis, bacillus coagulans, streptococcus thermophilus and bifidobacterium freeze-dried powder to prepare composite probiotics; mixing soybean protein, casein and whey protein powder to obtain composite protein;

Example 6

The embodiment provides a composition for nerve repair, which comprises the following components of a brain protein hydrolysate, a rehmannia root extract, a sea cucumber extract, a gastrodia elata extract, schisandra chinensis extract powder, licorice extract powder and nutritional aids in a mass ratio of 3:3:2.5:1:2:2:6, wherein the active component in the rehmannia root extract comprises catalpol in more than 70% by mass ratio, the active component in the sea cucumber extract comprises sea cucumber cerebroside and sea cucumber phospholipid in more than 80% by mass ratio, and the active component in the gastrodia elata extract comprises gastrodin, vanillin and gastrodia elata polysaccharide in more than 80% by mass ratio; the nutrition aid comprises the following components in percentage by mass of 1.5:0.3:1:1.2 composite vitamins, composite microelements, composite probiotics and composite proteins.

The compound vitamin consists of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E with equal mass. The composite protein comprises soybean protein and casein with equal quality. The compound probiotics comprise lactobacillus and lactobacillus reuteri with equal mass.

s1, respectively mixing the formula amounts of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E to prepare the compound vitamin; mixing iron fumarate, biological protein zinc, chromium picolinate, iodized salt, ammonium molybdate, malt selenium and copper sulfate to obtain a compound trace element; the lactobacillus and the lactobacillus reuteri freeze-dried powder are mixed to prepare the composite probiotics; mixing soybean protein and casein powder to obtain composite protein;

Comparative example 1

The difference from example 3 is that the composition 1 provided in this comparative example does not contain brain protein hydrolysate and the corresponding preparation method does not contain the step S3.

Comparative example 2

The difference from example 3 is that the composition 2 provided in this comparative example contains no nutritional aid as an ingredient. The preparation method of the composition 2 comprises the following steps:

s1, dissolving a brain protein hydrolysate raw material medicine in water, filtering by a nanofiltration membrane, and freeze-drying the filtrate to obtain brain protein hydrolysate;

s2, mixing and uniformly stirring the compound vitamin, the compound trace element, the coated compound probiotics and the brain protein hydrolysate according to the mass ratio to obtain the composition 2.

Comparative example 3

The difference from example 3 is that the composition 3 provided in this comparative example does not contain rehmannia root extract, sea cucumber extract, gastrodia tuber extract, schisandra fruit extract powder, licorice extract powder. The preparation method of the composition 3 comprises the following steps:

s3, mixing and uniformly stirring the compound vitamins, the compound trace elements, the coated compound probiotics and the brain protein hydrolysate according to the mass ratio to obtain the composition 3.

1. The compositions prepared in examples 1 to 6 were studied for their efficacy in mice model for Alzheimer's disease

AD model establishment, namely injecting a D galactose solution into the abdominal cavity of a mouse according to the dosage of 120mg/kg/D, and pouring a gastric aluminum trichloride solution according to the dosage of 10mg/kg/D, continuously feeding a model for 60D, then measuring the learning and memory capacity of the mouse by using a water maze, screening the AD mouse, and injecting an equivalent amount of physiological saline and a gastric equivalent amount of distilled water into a normal group.

AD model mice were divided into a blank group, experimental groups 1 to 6, and control groups 1 to 3 according to a random method in an animal grouping method, and 10 mice in one group were dosed in the following groups and doses, and the blank group and the normal group were injected with equal amounts of distilled water of the same amount as that of the stomach irrigation; experimental groups 1 to 6 respectively dissolved the compositions prepared in examples 1 to 6 in warm water at 35 to 40 ℃ and then filled with stomach, the dosage of the composition is 0.05g/kg/d, control groups 1 to 3 respectively dissolved the compositions prepared in examples 1 to 6 in warm water at 35 to 40 ℃ and then filled with stomach, the dosage of the composition is 0.5g/kg/d, wherein the administration time is 30d, and the administration of the molding compound is continued while the administration, so that natural recovery is avoided.

1. Open box experiment

And after grouping, carrying out open-box experimental tests on the experimental mice, wherein an experimental device for the open-box experiment comprises two parts, and the open-box device and a recording device. The height of the used open box is 30cm, the length and the width are 50cm, the hollow space is reserved, the peripheral wall is blackened, the upper part of the open box is connected with a camera, and the camera is connected with a computer so as to record experimental results. The bottom of the open box is divided into 25 blocks with the same area by using computer software, 16 blocks at the periphery of the open box are used as peripheral areas, and 9 blocks at the center are used as central areas. Each animal was assayed 1 time for 5min. The open memory experimental period was 5 days, the total distance of each mouse in the open box was recorded on the 6 th day of the experiment, and the residence time of the mice in the peripheral and central areas was calculated as the peripheral time ratio. After each cleaning of the open box, the test of the next mouse was performed.

2. Barnes maze test

Barnes maze test is carried out after open box experiment, the operation flow of the Banes maze test refers to the method of Barnes [9], and a little adjustment is carried out. The Barnes maze consists of a circular platform with 20 holes and a small, matt recess called the "target box" at the bottom of one of the holes, the prototype platform being 92cm in diameter and the small hole being 5cm in diameter. A camera is arranged above the maze and is connected with a computer. The mice were placed in the middle of the maze at the beginning of each training, the holes on the entry platform were freely selected, the time for each training was 4min, and the time taken for the mice to enter the target box within 4min (escape latency) was recorded. If the mice did not enter the target box within 4min, the escape latency was recorded as 240s for 6 days, the first 5 days as the maze memory test period, and the escape latency and error count data were recorded on day 6. After each mouse is done, the maze is wiped by alcohol, and the Barnes maze is rotated, but the relative spatial position of the target box is not changed, so that the mouse is prevented from finding the position of the target box through information such as smell.

The results of experiments 1 and 2 are shown in Table 1.

TABLE 1

2. The compositions prepared in examples 1 to 6 were investigated for their therapeutic effects on Alzheimer's disease

Comprises coating the compositions of examples 1, 3 and 6 with hydroxypropyl methylcellulose phthalate, and coating with soluble soybean polysaccharide at 50+ -5deg.C for 3 hr to obtain coating compositions 1, 3 and 6 with 15 mg/tablet.

100 patients with Alzheimer's disease are aged 50-70 years, 58 men and 42 women are aged 1-5 years, and have symptoms of disorder of behavior, aphasia, hypomnesis, slow action, unknown relatives, change of character and the like, and are clinically diagnosed as Alzheimer's disease. :

randomly dividing 100 patients with Alzheimer's disease into 5 groups, wherein the 1 group is placebo control group, and the 2 group is oral commercially available brain protein hydrolysate oral liquid, 50-100mg once and 3 times a day; groups 3 to 5 are oral administration of the coating compositions 1, 3, 6, 2 tablets at a time, 3 times a day.

Group 5 patients were treated in this manner for 3 months.

Standard of efficacy:

the effect is shown: consciousness, knowledge of relatives, directional soundness, normal action, basically correct answer to questions, self-care of life and general social activities;

it is effective in normal action, correct answer to questions, self-care, but slow reaction, and intelligence and personality remain in part.

The main clinical symptoms are not improved, life cannot be self-care, the answer of questions is incorrect, and the mind is dull.

Therapeutic results

The cure of each group of patients is shown in table 2, with effective rate = (effective number + effective number)/total number.

TABLE 2

	Has obvious effect	Effective and effective	Invalidation of	Effective rate of
					Placebo	0	1	19	5％
Oral liquid	5	9	6	70％
					Coating compound 1	12	5	3	85％
Coating compound 3	14	6	0	100％
					Coating compound 6	12	7	1	95％

Analysis and conclusion:

as can be seen from table 1, the total distance and the peripheral ratio of the walking of the experimental groups 1 to 6 are equivalent to those of the normal groups, the physical ability of each group of mice is not significantly different, and the peripheral time ratio is also equivalent, which indicates that the mental states of the experimental groups 1 to 6 mice are not significantly different from that of the normal groups of mice. In addition, the total walking distance and the surrounding time of the experimental groups 1-6 are slightly lower than those of the control groups 1-3, which shows that the mental state of the mice in the experimental groups 1-6 is slightly better than that of the control groups 1-3, and shows that the composition of the examples 1-6 has higher organism restoration force on AD model mice than that of the composition of the comparative examples 1-3, and has a synergistic effect among brain protein hydrolysate, rehmannia root extract, sea cucumber extract, gastrodia elata extract, schisandra chinensis extract powder, licorice extract powder and nutrition aid. The total distance and the surrounding ratio of the walking of the experimental groups 1-6 and the control groups 1-3 are far smaller than those of the blank groups, which indicates that the mental state of the mice in the blank groups is far inferior to those of the mice in the experimental groups 1-6 and the control groups 1-3, and the compositions of the examples 1-6 and the comparative examples 1-3 have the promotion effect on the mental state, the memory and the organism repair of the mice in the AD model. The latency and error times of the mice in the experimental groups 1-6 and the control groups 1-3 are far lower than those in the blank groups, which indicates that the learning and memory abilities and the cognitive functions of the mice in the experimental groups 1-6 and the control groups 1-3 are far better than those in the blank groups, the cognitive functions of the mice in the blank groups are seriously damaged, and the data in the experimental groups 1-6 are not obviously different from those in the normal groups, namely, the learning and memory abilities and the cognitive functions of the mice in the experimental groups 1-6 are not bad than those of the mice in the normal groups, which indicates that the composition of the embodiment 1-6 can effectively improve the cognitive disorder and the behavior disorder of the mice in the AD model, and improve the cognitive functions. The data for control groups 1-3 are superior to the blank, but the latency and number of errors are higher than those for experimental groups 1-6, indicating that the cognitive function of the mice in control groups 1-3 is weaker than that in experimental groups 1-6, and the improvement effect of the compositions of comparative examples 1-3 on cognitive disorders is lower than that of the compositions of examples 1-6. From the comparison of the data in table 1, the data of the mice in experimental group 3 are relatively superior, and it can be seen that the composition of example 3 is superior in improving cognitive function.

As can be seen from Table 2, the composition for nerve repair provided by the invention has an auxiliary treatment effect on Alzheimer's disease, and the effect is obviously better than that of a commercially available brain protein hydrolysate oral liquid.

The composition for nerve repair provided by the invention is prepared by synergetically matching the brain protein hydrolysate, the rehmannia root extract, the sea cucumber extract, the gastrodia elata extract, the schisandra chinensis extract powder, the licorice extract powder and the nutrition aid, wherein the brain protein hydrolysate is a peptide energy neurotrophic drug special for the brain, can act on central nerves, regulate and improve the metabolism of neurons, promote the formation of synapses, induce the differentiation of neurons, and further protect nerve cells from being damaged by various ischemia and neurotoxin. Catalpol in rehmanniae radix extract can reduce lipid peroxide content, increase activity of peroxide scavenger glutathione peroxidase, scavenge free radical, resist oxidation, resist PC12 apoptosis and signal mechanism caused by Abeta injury, reduce nerve cell apoptosis, promote hippocampal neurotrophic factor BDNF and signal molecule PKC expression, enhance hippocampal nerve plasticity, and promote nerve repair. The sea cucumber cephalin in the sea cucumber extract has a protective effect on nerve cells, can improve the activities of T-AOC and SOD, increase the antioxidation capability of cells, down regulate pro-apoptosis genes Bax, caspase-9 and Caspase-3, promote the expression of anti-apoptosis genes Bcl-2, inhibit nerve cell apoptosis, promote the growth of nerve cells, and protect the nerve cells from oxidative damage through the antioxidation activity and anti-apoptosis activity of the sea cucumber cephalin. The active ingredients of vanillin, gastrodin and gastrodia polysaccharide in the gastrodia elata extract can slow down apoptosis, show good anti-aging effect, remove free radicals of organisms, regulate an immune system and produce repairing effect on ischemic brain tissues by increasing the expression of brain-derived nerve factors and vascular endothelial growth factors. The schisandra polysaccharide in schisandra can be used for protecting the central nervous system by regulating neurotransmitter level in the Hippocampus, improving the stability of the central nervous system and promoting the protection and repair of the nervous system. Licochalcone A in Glycyrrhrizae radix has neurite growth promoting activity, neuron survival and differentiation promoting, nervous system stability maintaining and injured nerve repair promoting effects. There is also a communication between diseases related to the central nervous system and the intestinal flora, where metabolites, bacteriocins and neurotransmitters produced by microorganisms in the intestinal tract affect the excitability of neurons in the central nervous system via the vagus nerve, thereby affecting brain tissue, and the addition of complex probiotics can improve the intestinal flora of the body, which can modulate the central gamma-aminobutyric acid receptor via the vagus nerve thereby affecting brain neurochemistry (neurotransmitter levels, receptors and various neurotrophic factors) and behavior, improving nerve damage or apoptosis, and promoting nerve repair. Moreover, nerve damage and repair are also affected by nutritional levels, and most of nerve damage diseases, such as Alzheimer's disease and Parkinson's disease, usually the elderly, are mostly accompanied by intestinal mucosa atrophy, which affects nutrient absorption, and malnutrition, especially protein intake in food is reduced, and brain function damage and disease conditions are more serious. The addition of the compound probiotics, the compound vitamins, the compound microelements and the compound proteins not only increases the nutrition source, but also improves the intestinal tract absorption function, ensures the nutrition level of the organism, improves the immunity of the organism and promotes the organism to repair.

Therefore, the composition for nerve repair of the invention is prepared by synergetically matching brain protein hydrolysate, rehmannia root extract, sea cucumber extract, gastrodia elata extract, schisandra chinensis extract powder, licorice extract powder and nutritional aid, and can inhibit apoptosis of neurons and remove apoptosis of Abeta deposition of PC12 cells and a signaling mechanism caused by Abeta injury; 2. oxidation, slowing down neuronal aging; 3. increasing the expression of brain-derived nerve factor and vascular endothelial growth factor to repair ischemic brain tissue; 4. regulating neurotransmitter levels in the hippocampus to protect the central nervous system; 5. promoting the survival and differentiation of neurons, improving the stability of the central nervous system, and promoting the protection and repair of the nervous system; 6. improving intestinal flora, regulating central gamma-aminobutyric acid receptor by vagus nerve to influence brain nerve chemistry (neurotransmitter level, receptor and various neurotrophic factors) and behavior, improving nerve injury or apoptosis, and promoting nerve repair; 7. promoting nutrition absorption, ensuring organism nutrition level, improving organism immunity, promoting organism repair, etc., improving nervous system stability, protecting nervous system from injury, and promoting nervous system injury repair.

The present invention is not limited to the preferred embodiments, but is intended to cover modifications within the scope of the present invention as defined by the appended claims.

Claims

1. The application of the nerve repairing composition in preparing the nerve injury repairing medicine for Alzheimer's disease is characterized in that the nerve repairing composition comprises the following components of brain protein hydrolysate, rehmannia root extract, sea cucumber extract, gastrodia elata extract, schisandra chinensis extract powder, licorice extract powder and nutrition aid in a mass ratio of 1.8:2.5:3:0.6:1.4:2:5.5, wherein the active component in the rehmannia root extract comprises more than 70% of catalpol in mass ratio, the active component in the sea cucumber extract comprises more than 80% of sea cucumber cerebroside and sea cucumber phospholipid in mass ratio, and the active component in the gastrodia elata extract comprises more than 80% of gastrodin, vanillin and gastrodia elata polysaccharide in mass ratio; the nutrition aid comprises the following components in percentage by mass of 1.5:0.3:1:1.2 composite vitamins, composite microelements, composite probiotics and composite proteins;

the compound vitamin consists of vitamin B1, vitamin B12, vitamin C, vitamin D and vitamin E with equal mass; the composite protein comprises whey protein and pea protein with equal quality; the compound probiotics comprise lactobacillus, lactobacillus reuteri, clostridium butyricum, bacillus subtilis and bifidobacterium with equal mass; the compound microelements comprise iron, zinc, chromium, iodine, molybdenum, selenium and copper.