CN115381060A - Throat-moistening and lung-nourishing honey loquat leaf extract and preparation method thereof - Google Patents

Throat-moistening and lung-nourishing honey loquat leaf extract and preparation method thereof Download PDF

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CN115381060A
CN115381060A CN202110567833.6A CN202110567833A CN115381060A CN 115381060 A CN115381060 A CN 115381060A CN 202110567833 A CN202110567833 A CN 202110567833A CN 115381060 A CN115381060 A CN 115381060A
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loquat
honey
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缪婧
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Fujian Shenfeng Technology Development Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/10—Marmalades; Jams; Jellies; Other similar fruit or vegetable compositions; Simulated fruit products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/20—Products from apiculture, e.g. royal jelly or pollen; Substitutes therefor
    • A23L21/25—Honey; Honey substitutes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

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  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Jellies, Jams, And Syrups (AREA)
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Abstract

The invention discloses throat-moistening and lung-nourishing honey loquat leaf extract and a preparation method thereof, wherein the throat-moistening and lung-nourishing honey loquat leaf extract comprises a main material and an auxiliary material, and the main material comprises the following components in parts by weight: 58-80 parts of loquat leaf extract, 15-25 parts of mint extract, 100-150 parts of honey, 14-18 parts of poria cocos, 9-15 parts of flos farfarae, 3-8 parts of ginger, 4-9 parts of houttuynia cordata, 5-10 parts of radix peucedani, 9-17 parts of platycodon grandiflorum and 7-13 parts of momordica grosvenori; the auxiliary materials comprise the following components in parts by weight: 8-14 parts of maltose, 1-3 parts of sodium benzoate, 2-5 parts of almond essence and 1.5-5.5 parts of citric acid. The honey loquat leaf extract for wetting and nourishing the throat and the lung has good taste, has the effects of wetting the throat and clearing the lung, clearing heat and diffusing the lung, nourishing the lung, clearing heat and releasing toxin, relieving cough and reducing sputum, can also supplement nutrition, improve immunity and prevent cold, and has low production cost and good storage stability.

Description

Throat-moistening and lung-nourishing honey loquat leaf extract and preparation method thereof
Technical Field
The invention relates to the technical field of nutritional health-care foods, in particular to a honey loquat cream capable of moistening throat and nourishing lung and a preparation method thereof.
Background
According to statistics, nearly three hundred million people in China infect respiratory diseases every year, wherein cough patients can reach more than five thousand and more than ten thousand, and lungs are important components of respiratory systems of people, so that the protection of lung health is essential to people.
The loquat tastes sweet and tender, is not only a fruit, but also a medicinal fruit, is the crown of the fruit, has the efficacies of relieving cough and reducing sputum, clearing heat and moistening lung because of the amygdalin contained in the loquat, and is a main loquat producing country in the world, the cultivation and yield account for more than 2/3 of the world, and the resources are very rich. Loquat leaves, also called bayeye and kumquat leaves, have the effects of relieving cough and eliminating phlegm, have a certain treatment effect on chronic bronchitis, and loquat glycoside, ursolic acid and total triterpenic acid in the loquat leaves can obviously prolong the latent period of guinea pig cough caused by spraying of sulfur dioxide and citric acid, and obviously reduce the cough frequency.
The common honey loquat leaf extract in the market mostly takes bulbus fritilariae, loquat leaves or loquat leaves and honey as main raw materials, but at present, the loquat leaves are utilized more extensively, and most of the common honey loquat leaf extract is directly dried in the sun and then is directly decocted and extracted with water to be used as medicinal or edible raw materials.
Disclosure of Invention
In view of the above, the invention provides a honey loquat leaf extract for moistening throat and nourishing lung and a preparation method thereof, so as to solve the technical problems.
The invention provides the following technical scheme:
the throat-moistening and lung-nourishing honey loquat leaf extract comprises a main material and an auxiliary material, wherein the main material comprises the following components in parts by weight: 58-80 parts of loquat leaf extract, 15-25 parts of mint extract, 100-150 parts of honey, 14-18 parts of poria cocos, 9-15 parts of flos farfarae, 3-8 parts of ginger, 4-9 parts of houttuynia cordata, 5-10 parts of radix peucedani, 9-17 parts of platycodon grandiflorum and 7-13 parts of momordica grosvenori; the auxiliary materials comprise the following components in parts by weight: 8-14 parts of maltose, 1-3 parts of sodium benzoate, 2-5 parts of almond essence and 1.5-5.5 parts of citric acid.
Preferably, the honey loquat leaf extract for moistening and nourishing throat and lung comprises main materials and auxiliary materials, wherein the main materials comprise the following components in parts by weight: 65-77 parts of loquat leaf extract, 18-22 parts of mint extract, 110-140 parts of honey, 15-16 parts of poria cocos, 12-14 parts of flos farfarae, 5-7 parts of ginger, 5-8 parts of houttuynia cordata, 7-9 parts of radix peucedani, 12-15 parts of platycodon grandiflorum and 10-12 parts of momordica grosvenori; the auxiliary materials comprise the following components in parts by weight: 10-12 parts of maltose, 1.5-2.5 parts of sodium benzoate, 2.5-4.5 parts of almond essence and 2-4 parts of citric acid.
More preferably, the honey loquat leaf extract for moistening and nourishing throat and lung comprises a main material and an auxiliary material, wherein the main material comprises the following components in parts by weight: 70 parts of loquat leaf extract, 20 parts of mint extract, 120 parts of honey, 15.5 parts of tuckahoe, 13 parts of tussilago farfara 6 parts of ginger, 7 parts of houttuynia cordata, 8 parts of radix peucedani, 13 parts of platycodon grandiflorum and 11 parts of momordica grosvenori; the auxiliary materials comprise the following components in parts by weight: 11 parts of maltose, 2.5 parts of sodium benzoate, 3 parts of almond essence and 3.5 parts of citric acid.
Preferably, the preparation of the loquat leaf extract comprises the following steps:
s1, cleaning folium eriobotryae, and drying for 2h to 3h by using vacuum microwaves to reduce the water content of the folium eriobotryae to 6-12%; sending the dried loquat leaves into a high-speed medicine grinder, and grinding into 16 meshes for later use; refluxing and de-esterifying the crushed loquat leaves by using diethyl ether to obtain de-esterified loquat leaf powder;
s2, adding the degreased loquat leaf powder prepared in the step S1 into an ultrasonic cleaning machine, adding 6~8 times of volume of purified water, mixing to obtain an enzymolysis solution, controlling the enzymolysis solution to be at 45-55 ℃, adding cellulase, pectinase, alkaline protease and trypsin, adjusting the pH value to be 7.5-8.5, and performing ultrasonic compound enzymolysis for 2 times;
s3, after the ultrasonic complex enzymolysis is finished, inactivating enzymes at 95-100 ℃ for 10min-15min in stability, then carrying out suction filtration, decompressing and concentrating the filtrate, adding a 95% ethanol solution with the volume 5 times of that of the filtrate for precipitation, and then centrifuging at the rotating speed of 3000r/min for 10min-15min; centrifuging at the rotating speed of 6000r/min for 15min to 20min, then abandoning bottom filter residues, and reserving filtrate;
s4, preparing a chloroform-n-butanol mixed solution with the volume ratio of 4:1; mixing the filtrate obtained in the step S3 with a chloroform-n-butanol mixed solution according to a volume ratio of 4:1, stirring at a temperature of 45-50 ℃ for 30min at a rotating speed of 100-120 r/min, and centrifuging at a rotating speed of 3000r/min for 5min to obtain a honey filtrate for later use; adding a chloroform-n-butanol mixed solution with the volume of 1/4 of the filtrate into the filtrate, and repeating the above process for 5 times;
s5, taking the filtrate treated in the step S4, performing adsorption decoloration treatment by using a mixture of activated carbon and calcium chloride, and centrifuging at a rotating speed of 6000r/min for 10min to 15min; filtering the filtrate with a filter bag of 1 μ to obtain clear extractive solution of fructus Eriobotryae after centrifuging;
s6, subjecting the clear loquat extract obtained in the step S5 to reverse osmosis concentration equipment to concentrate the clear loquat extract;
and S7, putting the concentrated loquat extract obtained in the step S6 into a vacuum freeze dryer, and carrying out vacuum freeze drying for 12-24 hours to obtain a loquat leaf extract with the water content of less than 5%.
More preferably, in step S2, the cellulase, pectinase, alkaline protease and trypsin are added in the following order: 600U/g-800U/g, 1000U/g-1200U/g, 6000U/g-10000U/g, 3000U/g-7000U/g.
More preferably, in step S2, the process parameters of the ultrasonic composite enzymolysis are as follows: the enzymolysis temperature is 45-50 ℃, the enzymolysis time is 80min, and the sound field intensity is 30W/cm 2 ～40W/cm 2 The ultrasonic frequency is 20 kHz-25 kHz.
More preferably, in step S7, the setting temperature of the vacuum freeze dryer is-40 ℃ to-45 ℃ and the setting pressure is 10pa to 15pa.
Preferably, the mint extract comprises the following components in parts by weight: 60-80 parts of mint, 10-15 parts of honeysuckle, 3-10 parts of chrysanthemum and 6-10 parts of liquorice.
More preferably, the raw materials of the mint extract comprise the following components in parts by weight: 70 parts of mint, 13 parts of honeysuckle, 6 parts of chrysanthemum and 8 parts of liquorice.
More preferably, the mint extract is prepared by adopting a water extraction, concentration and alcohol precipitation process; the content of active ingredients in the mint extract is more than or equal to 98 percent, and the water content is less than or equal to 5 percent.
The invention also aims to provide a preparation method of the honey loquat leaf extract for moistening and nourishing throat and lung, which comprises the following steps:
1) Cleaning the poria cocos, the coltsfoot flower, the ginger, the houttuynia cordata, the peucedanum root, the platycodon grandiflorum and the momordica grosvenori in parts by weight, removing impurities, drying, mixing and grinding to 16 meshes to obtain mixed powder;
2) Placing the mixed powder obtained in the step 1) into an extraction tank, adding 4 times of purified water, controlling the temperature of the purified water to be 30-40 ℃, decocting for 2 hours, and filtering the decoction into a storage tank for later use; adding 2 times of purified water with the temperature of 30-40 ℃, decocting for 2 hours, and filtering decoction liquid into a storage tank for later use;
3) Combining the two decoctions in the step 2), filtering and concentrating into a concentrated solution; then carrying out alcohol precipitation on the concentrated solution for 4h to 6h by adopting an ethanol solution; mixing the concentrated solution after alcohol precipitation with the loquat leaf extract and the mint extract in parts by weight, and stirring until the concentrated solution is completely dissolved; then adding the sodium benzoate in parts by weight, and stirring until the sodium benzoate is completely dissolved; then adding the citric acid in parts by weight, and stirring until the citric acid is completely dissolved to obtain a mixed material;
4) Putting the honey in parts by weight into warm water at the temperature of 45-50 ℃, heating for 6-8 h in a water-separating manner, and filtering to obtain honey filtrate for later use; mixing the maltose with the weight part and 1.5 times of the purified water by volume, heating and decocting with slow fire to obtain thick syrup for later use; dissolving the almond essence in parts by weight in ethanol to prepare essence solution for later use;
5) Adding the honey filtrate obtained in the step 4) into the mixture obtained in the step 3), and stirring at the temperature of 55-65 ℃ for 30min to 50min; cooling to 45 ℃, adding the syrup and the essence solution obtained in the step 4), continuously stirring and uniformly mixing, and cooling to obtain a mixture;
6) Filtering the mixture obtained in the step 5) by using a 120-mesh sieve and a 200-mesh sieve, concentrating in vacuum, sterilizing, subpackaging, sealing and storing to obtain the honey loquat leaf extract for moistening the throat and nourishing the lung.
Preferably, in step 6), the process parameters of the vacuum concentration are as follows: the vacuum degree is 0.058MPa to 0.095MPa; the temperature is 30-35 ℃.
According to the technical scheme, the invention has the beneficial effects that:
the throat-moistening and lung-nourishing honey loquat leaf extract disclosed by the invention takes loquat leaf extract, mint extract, honey and other natural components as raw materials, and the loquat leaf has the effects of clearing heat, moistening lung, relieving cough and reducing sputum and the like; herba Menthae extract has effects in treating wind-heat type common cold, headache, conjunctival congestion, sore throat, and toothache; the honey has the effects of clearing heat, reducing pathogenic fire, clearing lung, relieving cough, loosening bowel to relieve constipation, expelling toxin, beautifying and the like; the honey loquat leaf extract collects the effects of the natural raw materials, and by using the unique extraction process and the preparation method, the effective components and active components in the raw materials are greatly preserved, and the honey loquat leaf extract does not contain pigments, additives and preservatives, is healthy and safe, and has no toxic or side effect. The honey loquat leaf extract for wetting and nourishing the throat and the lung has good taste, has the effects of wetting the throat and clearing the lung, clearing heat and diffusing the lung, nourishing the lung, clearing heat and releasing toxin, relieving cough and reducing sputum, can also supplement nutrition, improve immunity and prevent cold, and has low production cost and good storage stability.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is described in further detail below with reference to embodiments. The exemplary embodiments and descriptions of the present invention are provided to explain the present invention, but not to limit the present invention.
Example 1
Throat-moistening and lung-nourishing honey loquat leaf extract and preparation method thereof
The honey loquat leaf extract for wetting and nourishing the throat comprises main materials and auxiliary materials, wherein the main materials comprise the following components in parts by weight: 58kg of loquat leaf extract, 15kg of mint extract, 100kg of honey, 14kg of poria cocos, 9kg of flos farfarae, 3kg of ginger, 4kg of houttuynia cordata, 5kg of radix peucedani, 9kg of platycodon grandiflorum and 7kg of momordica grosvenori; the auxiliary materials comprise the following components in parts by weight: 8kg of maltose, 1kg of sodium benzoate, 2kg of almond essence and 1.5kg of citric acid.
The preparation method of the loquat leaf extract comprises the following steps:
s1, cleaning folium eriobotryae, and drying for 2 hours by using vacuum microwave to reduce the water content of the folium eriobotryae to 6%; sending the dried loquat leaves into a high-speed medicine grinder, and grinding into 16 meshes for later use; refluxing and de-esterifying the crushed loquat leaves by using diethyl ether to obtain de-esterified loquat leaf powder;
s2, adding the degreased loquat leaf powder prepared in the step S1 into an ultrasonic cleaning machine, adding purified water with the volume 6 times that of the degreased loquat leaf powder, mixing to obtain an enzymolysis solution, controlling the enzymolysis solution at 45 ℃, adding 600U/g cellulase, 1000U/g pectinase, 6000U/g alkaline protease and 3000U/g trypsin, adjusting the pH value to 7.5, and adjusting the temperature to 45 ℃ and the ultrasonic field intensity to 30W/cm 2 Carrying out ultrasonic compound enzymolysis for 2 times under the ultrasonic frequency of 20kHz, wherein the ultrasonic compound enzymolysis time is 80min each time;
s3, after the ultrasonic complex enzymolysis is finished, inactivating enzyme for 10min at 95 ℃ stably, then carrying out suction filtration and reduced pressure concentration on the filtrate, adding a 95% ethanol solution with 5 times of volume for precipitation, and then centrifuging for 10min at the rotating speed of 3000 r/min; centrifuging at 6000r/min for 15min, discarding the bottom residue, and collecting the filtrate;
s4, preparing a chloroform-n-butanol mixed solution with the volume ratio of 4:1; mixing the filtrate obtained in the step S3 with a chloroform-n-butanol mixed solution according to a volume ratio of 4:1, stirring at 45 ℃ and 100r/min for 30min, and centrifuging at 3000r/min for 5min to obtain a honey filtrate for later use; adding a chloroform-n-butanol mixed solution with the volume of 1/4 of the filtrate into the filtrate, and repeating the above process for 5 times;
s5, taking the filtrate treated in the step S4, performing adsorption decoloration treatment by using a mixture of activated carbon and calcium chloride, and then centrifuging at a rotating speed of 6000r/min for 10min; filtering the filtrate with a filter bag of 1 μ to obtain clear extractive solution of fructus Eriobotryae after centrifuging;
s6, subjecting the clear loquat extract obtained in the step S5 to reverse osmosis concentration equipment to concentrate the clear loquat extract;
and S7, putting the concentrated loquat extracting solution obtained in the step S6 into a vacuum freeze dryer, setting the temperature at minus 40 ℃ and the pressure at 10Pa, and carrying out vacuum freeze drying for 12h to obtain a loquat leaf extract with the water content of less than 5%.
The mint extract comprises the following raw materials in parts by weight: 60kg of mint, 10kg of honeysuckle, 3kg of chrysanthemum and 6kg of liquorice; the mint extract is prepared by adopting the process of water extraction, concentration and alcohol precipitation; the content of active ingredients in the mint extract is more than or equal to 98 percent, and the water content is less than or equal to 5 percent.
The preparation method of the honey loquat leaf extract for moistening and nourishing the throat and the lung comprises the following steps:
1) Cleaning the poria cocos, the coltsfoot flower, the ginger, the houttuynia cordata, the peucedanum praeruptorum, the platycodon grandiflorum and the momordica grosvenori in parts by weight, removing impurities, drying, mixing and grinding to 16 meshes to obtain mixed powder;
2) Placing the mixed powder obtained in the step 1) into an extraction tank, adding 4 times of purified water, controlling the temperature of the purified water to be 30 ℃, decocting for 2 hours, and filtering the decoction into a storage tank for later use; adding 2 times volume of purified water at 30 deg.C, decocting for 2 hr, filtering decoction, and storing in a storage tank;
3) Combining the two decoctions in the step 2), filtering and concentrating into a concentrated solution; precipitating the concentrated solution with ethanol for 4h; mixing the concentrated solution after alcohol precipitation with the loquat leaf extract and the mint extract in parts by weight, and stirring until the concentrated solution is completely dissolved; then adding the sodium benzoate in parts by weight, and stirring until the sodium benzoate is completely dissolved; then adding the citric acid in parts by weight, and stirring until the citric acid is completely dissolved to obtain a mixed material;
4) Adding the honey in parts by weight into warm water at 45 ℃ for heating for 6 hours in a water separating manner, and filtering to obtain honey filtrate for later use; mixing the maltose with the weight part and 1.5 times of the purified water by volume, heating and decocting with slow fire to obtain thick syrup for later use; dissolving the almond essence in parts by weight in ethanol to prepare essence solution for later use;
5) Adding the honey filtrate obtained in the step 4) into the mixture obtained in the step 3), and stirring for 30min at the temperature of 55 ℃; cooling to 45 ℃, adding the syrup and the essence solution obtained in the step 4), continuously stirring and uniformly mixing, and cooling to obtain a mixture;
6) Filtering the mixture obtained in the step 5) by using a 120-mesh sieve and a 200-mesh sieve, then carrying out vacuum concentration under the conditions of a vacuum degree of 0.058MPa and a temperature of 30 ℃, sterilizing, subpackaging, sealing and storing to obtain the throat-moistening and lung-nourishing honey loquat leaf extract.
Example 2
Throat-moistening and lung-nourishing honey loquat leaf extract and preparation method thereof
The honey loquat leaf extract for wetting and nourishing the throat and the lung comprises a main material and an auxiliary material,
the main material comprises the following components in parts by weight: 65kg of loquat leaf extract, 18kg of mint extract, 110kg of honey, 15kg of poria cocos, 12kg of tussilago farfara, 5kg of ginger, 5kg of houttuynia cordata, 7kg of radix peucedani, 12kg of platycodon grandiflorum and 10kg of momordica grosvenori; the auxiliary materials comprise the following components in parts by weight: 10kg of maltose, 1.5kg of sodium benzoate, 2.5kg of almond essence and 2kg of citric acid.
The preparation method of the loquat leaf extract comprises the following steps:
s1, cleaning folium eriobotryae, and drying for 2.5 hours by using vacuum microwave to reduce the water content of the folium eriobotryae to 8%; sending the dried loquat leaves into a high-speed medicine grinder, and grinding into 16 meshes for later use; refluxing and de-esterifying the crushed loquat leaves by using diethyl ether to obtain de-esterified loquat leaf powder;
s2, adding the degreased loquat leaf powder prepared in the step S1 into an ultrasonic cleaning machine, adding purified water with the volume 6 times that of the degreased loquat leaf powder, mixing to obtain an enzymolysis solution, controlling the enzymolysis solution at 45 ℃, adding 700U/g cellulase, 1100U/g pectinase, 7000U/g alkaline protease and 5000U/g trypsin, adjusting the pH value to 7.5, and adjusting the temperature at 45 ℃ and the ultrasonic field intensity at 35W/cm 2 Carrying out ultrasonic compound enzymolysis for 2 times under the ultrasonic frequency of 2225kHz, wherein the ultrasonic compound enzymolysis time is 80min each time;
s3, after the ultrasonic complex enzyme hydrolysis is finished, carrying out stable enzyme deactivation at 95 ℃ for 13min, then carrying out suction filtration and reduced pressure concentration on the filtrate, adding a 95% ethanol solution with 5 times of volume for precipitation, and then centrifuging for 12min at the rotating speed of 3000 r/min; centrifuging at 6000r/min for 17min, discarding the bottom residue, and collecting the filtrate;
s4, preparing a chloroform-n-butanol mixed solution with the volume ratio of 4:1; mixing the filtrate obtained in the step S3 with a chloroform-n-butanol mixed solution according to a volume ratio of 4:1, stirring at 45 ℃ and 100r/min for 30min, and centrifuging at 3000r/min for 5min to obtain a honey filtrate for later use; adding a chloroform-n-butanol mixed solution with the volume of 1/4 of the filtrate into the filtrate, and repeating the above process for 5 times;
s5, taking the filtrate treated in the step S4, performing adsorption decoloration treatment by using a mixture of activated carbon and calcium chloride, and then centrifuging at a rotating speed of 6000r/min for 12min; filtering the filtrate with a filter bag of 1 μ to obtain clear extractive solution of fructus Eriobotryae after centrifuging;
s6, taking the clarified loquat extracting solution obtained in the step S5, and concentrating the clarified loquat extracting solution through reverse osmosis concentration equipment;
and S7, putting the concentrated loquat extracting solution obtained in the step S6 into a vacuum freeze dryer, setting the temperature at minus 40 ℃ and the pressure at 11Pa, and carrying out vacuum freeze drying for 15h to obtain a loquat leaf extract with the water content of less than 5%.
The mint extract comprises the following raw materials in parts by weight: 65kg of mint, 12kg of honeysuckle, 5kg of chrysanthemum and 7kg of liquorice; the mint extract is prepared by adopting a water extraction, concentration and alcohol precipitation process; the content of effective components in the mint extract is more than or equal to 98 percent, and the water content is less than or equal to 5 percent.
The preparation method of the honey loquat leaf extract for moistening and nourishing the throat and the lung comprises the following steps:
1) Cleaning the poria cocos, the coltsfoot flower, the ginger, the houttuynia cordata, the peucedanum root, the platycodon grandiflorum and the momordica grosvenori in parts by weight, removing impurities, drying, mixing and grinding to 16 meshes to obtain mixed powder;
2) Placing the mixed powder obtained in the step 1) into an extraction tank, adding 4 times of purified water, controlling the temperature of the purified water to be 35 ℃, decocting for 2 hours, and filtering the decoction into a storage tank for later use; adding 2 times volume of purified water at 35 deg.C, decocting for 2 hr, filtering decoction, and storing in a tank;
3) Combining the two decoctions in the step 2), filtering and concentrating into a concentrated solution; precipitating the concentrated solution with ethanol for 5h; mixing the concentrated solution after alcohol precipitation with the loquat leaf extract and the mint extract in parts by weight, and stirring until the concentrated solution is completely dissolved; adding the sodium benzoate in the weight part, and stirring until the sodium benzoate is completely dissolved; then adding the citric acid in parts by weight, and stirring until the citric acid is completely dissolved to obtain a mixed material;
4) Adding the honey in parts by weight into warm water at 48 ℃ for heating for 6.5 hours in a water separating manner, and filtering to obtain honey filtrate for later use; mixing the maltose with the weight part with 1.5 times of the volume of purified water, heating and decocting with slow fire to be viscous, and preparing syrup for later use; dissolving the almond essence in parts by weight in ethanol to prepare essence solution for later use;
5) Adding the honey filtrate obtained in the step 4) into the mixture obtained in the step 3), and stirring at the temperature of 58 ℃ for 35min; cooling to 45 ℃, adding the syrup and the essence solution obtained in the step 4), continuously stirring and uniformly mixing, and cooling to obtain a mixture;
6) Filtering the mixture obtained in the step 5) with a 120-mesh sieve and a 200-mesh sieve, performing vacuum concentration under the conditions of a vacuum degree of 0.07MPa and a temperature of 30 ℃, sterilizing, subpackaging, sealing and storing to obtain the throat-moistening and lung-nourishing honey loquat leaf extract.
Example 3
Throat-moistening and lung-nourishing honey loquat leaf extract and preparation method thereof
The loquat leaf extract with honey for moistening and nourishing throat comprises main materials and auxiliary materials, and 7
The main material comprises the following components in parts by weight: 70kg of loquat leaf extract, 20kg of mint extract, 120kg of honey, 15.5kg of poria cocos, 13kg of flos farfarae, 6kg of ginger, 7kg of houttuynia cordata, 8kg of radix peucedani, 13kg of platycodon grandiflorum and 11kg of momordica grosvenori; the auxiliary materials comprise the following components in parts by weight: 11kg of maltose, 2.5kg of sodium benzoate, 3kg of almond essence and 3.5kg of citric acid.
The preparation method of the loquat leaf extract comprises the following steps:
s1, cleaning folium eriobotryae, and drying for 2.5 hours by using vacuum microwave to reduce the water content of the folium eriobotryae to 9%; sending the dried loquat leaves into a high-speed medicine grinder, and grinding into 16 meshes for later use; refluxing and de-esterifying the crushed loquat leaves by using diethyl ether to obtain de-esterified loquat leaf powder;
s2, adding the degreased loquat leaf powder prepared in the step S1 into an ultrasonic cleaning machine, adding purified water with the volume 6 times that of the degreased loquat leaf powder, mixing to obtain an enzymolysis solution, controlling the enzymolysis solution at 50 ℃, adding 800U/g cellulase, 1000U/g pectinase, 8000U/g alkaline protease and 5000U/g trypsin, adjusting the pH value to 8, and adjusting the temperature to 50 ℃ and the ultrasonic field intensity to 35W/cm 2 Carrying out ultrasonic compound enzymolysis for 2 times under the ultrasonic frequency of 25kHz, wherein the ultrasonic compound enzymolysis time is 80min each time;
s3, after the ultrasonic complex enzyme hydrolysis is finished, carrying out stable enzyme deactivation at 95 ℃ for 15min, then carrying out suction filtration and reduced pressure concentration on the filtrate, adding a 95% ethanol solution with 5 times of volume for precipitation, and then centrifuging at the rotating speed of 3000r/min for 10min; centrifuging at 6000r/min for 15min, discarding the bottom residue, and collecting the filtrate;
s4, preparing a chloroform-n-butanol mixed solution with the volume ratio of 4:1; mixing the filtrate obtained in the step S3 with a chloroform-n-butanol mixed solution according to a volume ratio of 4:1, stirring at 50 ℃ for 30min at a rotating speed of 100r/min, and centrifuging at a rotating speed of 3000r/min for 5min to obtain a honey filtrate for later use; adding a chloroform-n-butanol mixed solution with the volume of 1/4 of the filtrate into the filtrate, and repeating the above process for 5 times;
s5, taking the filtrate treated in the step S4, performing adsorption decoloration treatment by using a mixture of activated carbon and calcium chloride, and then centrifuging at a rotating speed of 6000r/min for 12min; after the centrifugation is finished, taking the filtrate and filtering the filtrate by a filter bag with the diameter of 1 mu to obtain clear loquat extracting solution;
s6, taking the clarified loquat extracting solution obtained in the step S5, and concentrating the clarified loquat extracting solution through reverse osmosis concentration equipment;
s7, putting the concentrated loquat extract obtained in the step S6 into a vacuum freeze dryer, setting the temperature at minus 40 ℃ and the pressure at 12Pa, and carrying out vacuum freeze drying for 18h to obtain the loquat leaf extract with the water content of less than 5%.
The mint extract comprises the following raw materials in parts by weight: the mint extract comprises the following components in kg by weight: 70kg of mint, 13kg of honeysuckle, 6kg of chrysanthemum and 8kg of liquorice; the mint extract is prepared by adopting a water extraction, concentration and alcohol precipitation process; the content of active ingredients in the mint extract is more than or equal to 98 percent, and the water content is less than or equal to 5 percent.
The preparation method of the honey loquat leaf extract for moistening and nourishing the throat and the lung comprises the following steps:
1) Cleaning the poria cocos, the coltsfoot flower, the ginger, the houttuynia cordata, the peucedanum root, the platycodon grandiflorum and the momordica grosvenori in parts by weight, removing impurities, drying, mixing and grinding to 16 meshes to obtain mixed powder;
2) Placing the mixed powder obtained in the step 1) into an extraction tank, adding 4 times of purified water, controlling the temperature of the purified water to be 40 ℃, decocting for 2 hours, and filtering the decoction into a storage tank for later use; adding 2 times volume of purified water at 40 deg.C, decocting for 2 hr, filtering decoction, and storing in a tank;
3) Combining the two decoctions in the step 2), filtering and concentrating into a concentrated solution; precipitating the concentrated solution with ethanol for 5h; mixing the concentrated solution after alcohol precipitation with the loquat leaf extract and the mint extract in parts by weight, and stirring until the concentrated solution is completely dissolved; then adding the sodium benzoate in parts by weight, and stirring until the sodium benzoate is completely dissolved; then adding the citric acid in parts by weight, and stirring until the citric acid is completely dissolved to obtain a mixed material;
4) Adding the honey in the weight parts into warm water at 45 ℃ and heating for 7 hours in a water separating manner, and filtering to obtain honey filtrate for later use; mixing the maltose with the weight part with 1.5 times of the volume of purified water, heating and decocting with slow fire to be viscous, and preparing syrup for later use; dissolving the almond essence in parts by weight by using ethanol to prepare essence solution for later use;
5) Adding the honey filtrate obtained in the step 4) into the mixture obtained in the step 3), and stirring at 60 ℃ for 40min; cooling to 45 ℃, adding the syrup and the essence solution obtained in the step 4), continuously stirring and uniformly mixing, and cooling to obtain a mixture;
6) Filtering the mixture obtained in the step 5) with a 120-mesh sieve and a 200-mesh sieve in a double-layer manner, then carrying out vacuum concentration under the conditions of a vacuum degree of 0.065MPa and a temperature of 30 ℃, sterilizing, subpackaging, and sealing and storing to obtain the throat-moistening and lung-nourishing honey loquat ointment.
Example 4
Throat-moistening and lung-nourishing honey loquat leaf extract and preparation method thereof
The honey loquat leaf extract for wetting and nourishing the throat and the lung comprises a main material and an auxiliary material,
the main material comprises the following components in parts by weight: 77kg of loquat leaf extract, 22kg of mint extract, 140kg of honey, 16kg of poria cocos, 14kg of tussilago farfara, 7kg of ginger, 8kg of houttuynia cordata, 9kg of radix peucedani, 15kg of platycodon grandiflorum and 12kg of momordica grosvenori; the auxiliary materials comprise the following components in parts by weight: 12kg of maltose, 2.5kg of sodium benzoate, 4.5kg of almond essence and 4kg of citric acid.
The preparation method of the loquat leaf extract comprises the following steps:
s1, cleaning folium eriobotryae, and drying for 2.5 hours by using vacuum microwave to reduce the water content of the folium eriobotryae to 10%; sending the dried loquat leaves into a high-speed medicine grinder, and grinding into 16 meshes for later use; refluxing and de-esterifying the crushed loquat leaves by using diethyl ether to obtain de-esterified loquat leaf powder;
s2, adding the de-esterified loquat leaf powder prepared in the step S1 into an ultrasonic cleaning machine, adding purified water with the volume 7 times that of the de-esterified loquat leaf powder, mixing to obtain an enzymolysis solution, controlling the enzymolysis solution at 50 ℃,adding 700U/g cellulase, 1100U/g pectinase, 9000U/g alkaline protease and 6000U/g trypsin, adjusting pH to 8, and ultrasonic field intensity at 48 deg.C and 38W/cm 2 Carrying out ultrasonic compound enzymolysis for 2 times under the ultrasonic frequency of 24kHz, wherein the ultrasonic compound enzymolysis time is 80min each time;
s3, after the ultrasonic complex enzyme hydrolysis is finished, inactivating enzymes stably for 14min at 100 ℃, then carrying out suction filtration and reduced pressure concentration on the filtrate, adding a 95% ethanol solution with 5 times of volume for precipitation, and then centrifuging for 14min at the rotating speed of 3000 r/min; centrifuging at 6000r/min for 18min, discarding the bottom residue, and collecting the filtrate;
s4, preparing a chloroform-n-butanol mixed solution with the volume ratio of 4:1; mixing the filtrate obtained in the step S3 with a chloroform-n-butanol mixed solution according to a volume ratio of 4:1, stirring at 48 ℃ for 30min at a rotating speed of 110r/min, and centrifuging at a rotating speed of 3000r/min for 5min to obtain a honey filtrate for later use; adding a chloroform-n-butanol mixed solution with the volume of 1/4 of the filtrate into the filtrate, and repeating the above process for 5 times;
s5, taking the filtrate treated in the step S4, performing adsorption decoloration treatment by using a mixture of activated carbon and calcium chloride, and then centrifuging at a rotating speed of 6000r/min for 14min; filtering the filtrate with a filter bag of 1 μ to obtain clear extractive solution of fructus Eriobotryae after centrifuging;
s6, taking the clarified loquat extracting solution obtained in the step S5, and concentrating the clarified loquat extracting solution through reverse osmosis concentration equipment;
and S7, putting the concentrated loquat extracting solution obtained in the step S6 into a vacuum freeze dryer, setting the temperature at minus 45 ℃ and the pressure at 14Pa, and carrying out vacuum freeze drying for 20h to obtain a loquat leaf extract with the water content of less than 5%.
The mint extract comprises the following raw materials in parts by weight: 75kg of mint, 13kg of honeysuckle, 8kg of chrysanthemum and 8kg of liquorice; the mint extract is prepared by adopting the process of water extraction, concentration and alcohol precipitation; the content of effective components in the mint extract is more than or equal to 98 percent, and the water content is less than or equal to 5 percent.
The preparation method of the honey loquat leaf extract for moistening and nourishing the throat and the lung comprises the following steps:
1) Cleaning the poria cocos, the coltsfoot flower, the ginger, the houttuynia cordata, the peucedanum root, the platycodon grandiflorum and the momordica grosvenori in parts by weight, removing impurities, drying, mixing and grinding to 16 meshes to obtain mixed powder;
2) Placing the mixed powder obtained in the step 1) into an extraction tank, adding 4 times of purified water, controlling the temperature of the purified water to be 38 ℃, decocting for 2 hours, and filtering the decoction into a storage tank for later use; adding 2 times volume of purified water at 38 deg.C, decocting for 2 hr, filtering decoction, and storing in a tank;
3) Combining the two decoctions in the step 2), filtering and concentrating into a concentrated solution; precipitating the concentrated solution with ethanol for 5.5h; mixing the concentrated solution after alcohol precipitation with the loquat leaf extract and the mint extract in parts by weight, and stirring until the concentrated solution is completely dissolved; then adding the sodium benzoate in parts by weight, and stirring until the sodium benzoate is completely dissolved; then adding the citric acid in parts by weight, and stirring until the citric acid is completely dissolved to obtain a mixed material;
4) Adding the honey in parts by weight into warm water at 48 ℃ for heating for 7.5 hours in a water separating manner, and filtering to obtain honey filtrate for later use; mixing the maltose with the weight part and 1.5 times of the purified water by volume, heating and decocting with slow fire to obtain thick syrup for later use; dissolving the almond essence in parts by weight in ethanol to prepare essence solution for later use;
5) Adding the honey filtrate obtained in the step 4) into the mixture obtained in the step 3), and stirring for 45min at the temperature of 60 ℃; cooling to 45 ℃, adding the syrup and the essence solution obtained in the step 4), continuously stirring and uniformly mixing, and cooling to obtain a mixture;
6) Filtering the mixture obtained in the step 5) with a 120-mesh sieve and a 200-mesh sieve, vacuum concentrating under the conditions of vacuum degree of 0.085MPa and temperature of 32 ℃, sterilizing, subpackaging, sealing and storing to obtain the throat-moistening and lung-nourishing honey loquat leaf extract.
Example 5
Throat-moistening and lung-nourishing honey loquat leaf extract and preparation method thereof
The honey loquat leaf extract for wetting and nourishing the throat comprises main materials and auxiliary materials, wherein the main materials comprise the following components in parts by weight: 80kg of loquat leaf extract, 25kg of mint extract, 150kg of honey, 18kg of poria cocos, 15kg of coltsfoot flower, 8kg of ginger, 9kg of houttuynia cordata, 10kg of radix peucedani, 17kg of platycodon grandiflorum and 13kg of momordica grosvenori; the auxiliary materials comprise the following components in parts by weight: 14kg of maltose, 3kg of sodium benzoate, 5kg of almond essence and 5.5kg of citric acid.
The preparation method of the loquat leaf extract comprises the following steps:
s1, cleaning folium eriobotryae, and drying for 2h to 3h by using vacuum microwaves to reduce the water content of the folium eriobotryae to 6-12%; sending the dried loquat leaves into a high-speed medicine grinder, and grinding into 16 meshes for later use; refluxing and de-esterifying the crushed loquat leaves by using diethyl ether to obtain de-esterified loquat leaf powder;
s2, adding the degreased loquat leaf powder prepared in the step S1 into an ultrasonic cleaning machine, adding 8 times of purified water for mixing to obtain an enzymolysis solution, controlling the enzymolysis solution to be 55 ℃, adding 800U/g of cellulase, 1200U/g of pectinase, 10000U/g of alkaline protease and 7000U/g of trypsin, adjusting the pH value to be 8.5, and controlling the temperature to be 50 ℃ and the ultrasonic sound field intensity to be 40W/cm 2 Carrying out ultrasonic compound enzymolysis for 2 times under the ultrasonic frequency of 25kHz, wherein the ultrasonic compound enzymolysis time is 80min each time;
s3, after the ultrasonic complex enzymolysis is finished, inactivating enzyme stably for 15min at 100 ℃, then carrying out suction filtration and reduced pressure concentration on the filtrate, adding a 95% ethanol solution with 5 times of volume for precipitation, and then centrifuging for 15min at the rotating speed of 3000 r/min; centrifuging at 6000r/min for 20min, discarding the bottom residue, and collecting the filtrate;
s4, preparing a chloroform-n-butanol mixed solution with the volume ratio of 4:1; mixing the filtrate obtained in the step S3 with a chloroform-n-butanol mixed solution according to a volume ratio of 4:1, stirring at 50 ℃ for 30min at a rotating speed of 120r/min, and centrifuging at a rotating speed of 3000r/min for 5min to obtain a honey filtrate for later use; adding a chloroform-n-butanol mixed solution with the volume of 1/4 of the filtrate into the filtrate, and repeating the above process for 5 times;
s5, taking the filtrate treated in the step S4, performing adsorption decoloration treatment by using a mixture of activated carbon and calcium chloride, and then centrifuging at a rotating speed of 6000r/min for 15min; filtering the filtrate with a filter bag of 1 μ to obtain clear extractive solution of fructus Eriobotryae after centrifuging;
s6, taking the clarified loquat extracting solution obtained in the step S5, and concentrating the clarified loquat extracting solution through reverse osmosis concentration equipment;
and S7, putting the concentrated loquat extracting solution obtained in the step S6 into a vacuum freeze dryer, setting the temperature at minus 45 ℃ and the pressure at 15Pa, and carrying out vacuum freeze drying for 24h to obtain a loquat leaf extract with the water content of less than 5%.
The mint extract comprises the following raw materials in parts by weight: 80kg of mint, 15kg of honeysuckle, 10kg of chrysanthemum and 10kg of liquorice; the mint extract is prepared by adopting a water extraction, concentration and alcohol precipitation process; the content of effective components in the mint extract is more than or equal to 98 percent, and the water content is less than or equal to 5 percent.
The preparation method of the honey loquat leaf extract for moistening and nourishing the throat and the lung comprises the following steps:
1) Cleaning the poria cocos, the coltsfoot flower, the ginger, the houttuynia cordata, the peucedanum root, the platycodon grandiflorum and the momordica grosvenori in parts by weight, removing impurities, drying, mixing and grinding to 16 meshes to obtain mixed powder;
2) Placing the mixed powder obtained in the step 1) into an extraction tank, adding 4 times of purified water, controlling the temperature of the purified water to be 40 ℃, decocting for 2 hours, and filtering the decoction into a storage tank for later use; adding 2 times volume of purified water at 40 deg.C, decocting for 2 hr, filtering decoction, and storing in a tank;
3) Combining the two decoctions in the step 2), filtering and concentrating into a concentrated solution; precipitating the concentrated solution with ethanol solution for 6h; mixing the concentrated solution after alcohol precipitation with the loquat leaf extract and the mint extract in parts by weight, and stirring until the concentrated solution is completely dissolved; adding the sodium benzoate in the weight part, and stirring until the sodium benzoate is completely dissolved; then adding the citric acid in parts by weight, and stirring until the citric acid is completely dissolved to obtain a mixed material;
4) Adding the honey in parts by weight into warm water at 50 ℃ for heating for 8 hours in a water separating manner, and filtering to obtain honey filtrate for later use; mixing the maltose with the weight part and 1.5 times of the purified water by volume, heating and decocting with slow fire to obtain thick syrup for later use; dissolving the almond essence in parts by weight by using ethanol to prepare essence solution for later use;
5) Adding the honey filtrate obtained in the step 4) into the mixture obtained in the step 3), and stirring at 65 ℃ for 50min; cooling to 45 ℃, adding the syrup and the essence solution obtained in the step 4), continuously stirring and uniformly mixing, and cooling to obtain a mixture;
6) Filtering the mixture obtained in the step 5) with a 120-mesh sieve and a 200-mesh sieve, performing vacuum concentration under the conditions of a vacuum degree of 0.095MPa and a temperature of 35 ℃, sterilizing, subpackaging, sealing and storing to obtain the throat-moistening and lung-nourishing honey loquat leaf extract.
The above description is only a preferred embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes may be made to the embodiment of the present invention by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims (10)

1. The throat-moistening and lung-nourishing honey loquat leaf extract is characterized by comprising main materials and auxiliary materials, wherein the main materials comprise the following components in parts by weight: 58-80 parts of loquat leaf extract, 15-25 parts of mint extract, 100-150 parts of honey, 14-18 parts of poria cocos, 9-15 parts of flos farfarae, 3-8 parts of ginger, 4-9 parts of houttuynia cordata, 5-10 parts of radix peucedani, 9-17 parts of platycodon grandiflorum and 7-13 parts of momordica grosvenori; the auxiliary materials comprise the following components in parts by weight: 8-14 parts of maltose, 1-3 parts of sodium benzoate, 2-5 parts of almond essence and 1.5-5.5 parts of citric acid.
2. The honey loquat cream for wetting and nourishing the throat and the lung according to claim 1, wherein the honey loquat cream for wetting and nourishing the throat comprises a main material and an auxiliary material, and the main material comprises the following components in parts by weight: 70 parts of loquat leaf extract, 20 parts of mint extract, 120 parts of honey, 15.5 parts of poria cocos, 13 parts of flos farfarae, 6 parts of ginger, 7 parts of houttuynia cordata, 8 parts of radix peucedani, 13 parts of platycodon grandiflorum and 11 parts of momordica grosvenori; the auxiliary materials comprise the following components in parts by weight: 11 parts of maltose, 2.5 parts of sodium benzoate, 3 parts of almond essence and 3.5 parts of citric acid.
3. The honey loquat cream for wetting and nourishing throat and lung according to claim 1, wherein the loquat leaf extract is prepared by the steps of:
s1, cleaning loquat leaves, and drying the loquat leaves for 2h to 3h by using vacuum microwaves to reduce the water content of the loquat leaves to 6 percent to 12 percent; sending the dried loquat leaves into a high-speed medicine grinder, and grinding into 16 meshes for later use; refluxing and de-esterifying the crushed loquat leaves by using diethyl ether to obtain de-esterified loquat leaf powder;
s2, adding the degreased loquat leaf powder prepared in the step S1 into an ultrasonic cleaning machine, adding 6~8 times of volume of purified water, mixing to obtain an enzymolysis solution, controlling the enzymolysis solution to be at 45-55 ℃, adding cellulase, pectinase, alkaline protease and trypsin, adjusting the pH value to be 7.5-8.5, and performing ultrasonic compound enzymolysis for 2 times;
s3, after the ultrasonic complex enzymolysis is finished, inactivating enzymes at 95-100 ℃ for 10min-15min in stability, then carrying out suction filtration, decompressing and concentrating the filtrate, adding a 95% ethanol solution with the volume 5 times of that of the filtrate for precipitation, and then centrifuging at the rotating speed of 3000r/min for 10min-15min; centrifuging at the rotating speed of 6000r/min for 15min to 20min, then abandoning bottom filter residues, and reserving filtrate;
s4, preparing a chloroform-n-butanol mixed solution with the volume ratio of 4:1; mixing the filtrate obtained in the step S3 with a chloroform-n-butanol mixed solution according to a volume ratio of 4:1, stirring at a temperature of 45-50 ℃ and a rotating speed of 100-120 r/min for 30min, and then centrifuging at a rotating speed of 3000r/min for 5min to obtain a honey filtrate for later use; adding a chloroform-n-butanol mixed solution with the volume of 1/4 of the filtrate into the filtrate, and repeating the above process for 5 times;
s5, taking the filtrate treated in the step S4, performing adsorption decoloration treatment by using a mixture of activated carbon and calcium chloride, and centrifuging at a rotating speed of 6000r/min for 10min to 15min; filtering the filtrate with a filter bag of 1 μ to obtain clear extractive solution of fructus Eriobotryae after centrifuging;
s6, taking the clarified loquat extracting solution obtained in the step S5, and concentrating the clarified loquat extracting solution through reverse osmosis concentration equipment;
and S7, putting the concentrated loquat extracting solution obtained in the step S6 into a vacuum freeze dryer, and carrying out vacuum freeze drying for 12-24 hours to obtain a loquat leaf extract with the water content of less than 5%.
4. The honey loquat cream for wetting the throat and nourishing the lung according to claim 3, wherein in the step S2, the cellulase, the pectinase, the alkaline protease and the trypsin are added in the following order: 600U/g-800U/g, 1000U/g-1200U/g, 6000U/g-10000U/g, 3000U/g-7000U/g.
5. The honey loquat cream for wetting and nourishing throat and lung according to claim 3, wherein in the step S2, the technological parameters of the ultrasonic composite enzymolysis are as follows: the enzymolysis temperature is 45-50 ℃, the enzymolysis time is 80min, and the sound field intensity is 30W/cm 2 ～40W/cm 2 The ultrasonic frequency is 20 kHz-25 kHz.
6. The throat-moistening and lung-nourishing honey loquat paste according to claim 3, wherein in the step S7, the setting temperature of the vacuum freeze dryer is-40 ℃ to-45 ℃, and the setting pressure is 10Pa to 15Pa.
7. The honey loquat cream for wetting and nourishing the throat and the lung according to claim 1, wherein the mint extract is prepared from the following raw materials in parts by weight: 60-80 parts of mint, 10-15 parts of honeysuckle, 3-10 parts of chrysanthemum and 6-10 parts of liquorice.
8. The honey loquat cream for wetting and nourishing the throat and the lung according to claim 7, wherein the mint extract is prepared by a water extraction, concentration and alcohol precipitation process; the content of effective components in the mint extract is more than or equal to 98 percent, and the water content is less than or equal to 5 percent.
9. The preparation method of the honey loquat cream for moistening and nourishing the throat and the lung according to any one of the claims 1~8, comprising the steps of:
1) Cleaning the poria cocos, the coltsfoot flower, the ginger, the houttuynia cordata, the peucedanum root, the platycodon grandiflorum and the momordica grosvenori in parts by weight, removing impurities, drying, mixing and grinding to 16 meshes to obtain mixed powder;
2) Placing the mixed powder obtained in the step 1) into an extraction tank, adding 4 times of purified water, controlling the temperature of the purified water to be 30-40 ℃, decocting for 2 hours, and filtering the decoction into a storage tank for later use; adding 2 times of purified water with the temperature of 30-40 ℃, decocting for 2 hours, and filtering decoction liquid into a storage tank for later use;
3) Mixing the two decoctions obtained in the step 2), filtering and concentrating to obtain a concentrated solution; then carrying out alcohol precipitation on the concentrated solution for 4h to 6h by adopting an ethanol solution; mixing the concentrated solution after alcohol precipitation with the loquat leaf extract and the mint extract in parts by weight, and stirring until the concentrated solution is completely dissolved; then adding the sodium benzoate in parts by weight, and stirring until the sodium benzoate is completely dissolved; then adding the citric acid in parts by weight, and stirring until the citric acid is completely dissolved to obtain a mixed material;
4) Putting the honey in parts by weight into warm water at the temperature of 45-50 ℃, heating for 6-8 h in a water-separating manner, and filtering to obtain honey filtrate for later use; mixing the maltose with the weight part and 1.5 times of the purified water by volume, heating and decocting with slow fire to obtain thick syrup for later use; dissolving the almond essence in parts by weight in ethanol to prepare essence solution for later use;
5) Adding the honey filtrate obtained in the step 4) into the mixture obtained in the step 3), and stirring at the temperature of 55-65 ℃ for 30min to 50min; cooling to 45 ℃, adding the syrup and the essence solution obtained in the step 4), continuously stirring and uniformly mixing, and cooling to obtain a mixture;
6) Filtering the mixture obtained in the step 5) by using a 120-mesh sieve and a 200-mesh sieve, concentrating in vacuum, sterilizing, subpackaging, sealing and storing to obtain the honey loquat leaf extract for moistening the throat and nourishing the lung.
10. The preparation method of honey loquat cream for wetting and nourishing throat and lung according to claim 9, wherein in step 6), the vacuum concentration process parameters are as follows: the vacuum degree is 0.058MPa to 0.095MPa; the temperature is 30-35 ℃.
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Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1242219A (en) * 1999-06-02 2000-01-26 戴振先 Traditional Chinese medicine soft extract with bee honey for removing dust from lungs
CN1788782A (en) * 2004-12-16 2006-06-21 神奇梵净山铜仁药业有限公司 Loquat leaf extractum for nourishing lung and relieving cough
CN101874882A (en) * 2010-06-21 2010-11-03 江西滕王阁药业有限公司 Strong loquat leaf extract (blended with honey) and preparation method thereof
CN104231096A (en) * 2013-06-13 2014-12-24 段玉虎 Ultrasonic-assisted method for degreasing and extracting potentilla anserina polysaccharide
CN104305457A (en) * 2014-11-14 2015-01-28 广西壮族自治区农业科学院农产品加工研究所 Preparation method of cumquat and loquat extract
CN107296924A (en) * 2017-08-16 2017-10-27 黄山市歙县绿色三潭枇杷生物科技有限公司 A kind of loquat leaf extract and preparation method thereof
CN107455709A (en) * 2017-08-09 2017-12-12 安徽省奇圣养蜂专业合作社 A kind of Momordica grosvenori cough-relieving honey paste and preparation method thereof
CN108065017A (en) * 2016-11-14 2018-05-25 威海紫光生物科技开发有限公司 A kind of health-care food composition of clearing
CN109125258A (en) * 2018-10-25 2019-01-04 华润三九（南昌）药业有限公司 A kind of preparation method of intensified loquet distillate
CN111011794A (en) * 2019-11-06 2020-04-17 福建省神蜂科技开发有限公司 Extraction process of honey loquat leaf extract

Patent Citations (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1242219A (en) * 1999-06-02 2000-01-26 戴振先 Traditional Chinese medicine soft extract with bee honey for removing dust from lungs
CN1788782A (en) * 2004-12-16 2006-06-21 神奇梵净山铜仁药业有限公司 Loquat leaf extractum for nourishing lung and relieving cough
CN101874882A (en) * 2010-06-21 2010-11-03 江西滕王阁药业有限公司 Strong loquat leaf extract (blended with honey) and preparation method thereof
CN104231096A (en) * 2013-06-13 2014-12-24 段玉虎 Ultrasonic-assisted method for degreasing and extracting potentilla anserina polysaccharide
CN104305457A (en) * 2014-11-14 2015-01-28 广西壮族自治区农业科学院农产品加工研究所 Preparation method of cumquat and loquat extract
CN108065017A (en) * 2016-11-14 2018-05-25 威海紫光生物科技开发有限公司 A kind of health-care food composition of clearing
CN107455709A (en) * 2017-08-09 2017-12-12 安徽省奇圣养蜂专业合作社 A kind of Momordica grosvenori cough-relieving honey paste and preparation method thereof
CN107296924A (en) * 2017-08-16 2017-10-27 黄山市歙县绿色三潭枇杷生物科技有限公司 A kind of loquat leaf extract and preparation method thereof
CN109125258A (en) * 2018-10-25 2019-01-04 华润三九（南昌）药业有限公司 A kind of preparation method of intensified loquet distillate
CN111011794A (en) * 2019-11-06 2020-04-17 福建省神蜂科技开发有限公司 Extraction process of honey loquat leaf extract

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
何传波;魏好程;熊何健;吴国宏;: "枇杷叶多糖酶法提取工艺优化及其离子交换层析纯化", 食品科学, no. 08, pages 45 - 50 *

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