CN115380995A - Bacterium enzyme acid composite preparation and application thereof - Google Patents

Bacterium enzyme acid composite preparation and application thereof Download PDF

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CN115380995A
CN115380995A CN202210877468.3A CN202210877468A CN115380995A CN 115380995 A CN115380995 A CN 115380995A CN 202210877468 A CN202210877468 A CN 202210877468A CN 115380995 A CN115380995 A CN 115380995A
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feed
fusarium
parts
preparation
suspension
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CN115380995B (en
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徐丽
周樱
张成杰
苏丹
陈雪姣
邓晓旭
程瑛
程超
辜玲芳
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Wuhan Sunhy Biological Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K30/00—Processes specially adapted for preservation of materials in order to produce animal feeding-stuffs
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3481—Organic compounds containing oxygen
    • A23L3/3508—Organic compounds containing oxygen containing carboxyl groups
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L3/00—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs
    • A23L3/34—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals
    • A23L3/3454—Preservation of foods or foodstuffs, in general, e.g. pasteurising, sterilising, specially adapted for foods or foodstuffs by treatment with chemicals in the form of liquids or solids
    • A23L3/3463—Organic compounds; Microorganisms; Enzymes
    • A23L3/3571—Microorganisms; Enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/25—Removal of unwanted matter, e.g. deodorisation or detoxification using enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/27—Removal of unwanted matter, e.g. deodorisation or detoxification by chemical treatment, by adsorption or by absorption
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • A23L5/20—Removal of unwanted matter, e.g. deodorisation or detoxification
    • A23L5/28—Removal of unwanted matter, e.g. deodorisation or detoxification using microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
    • Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
    • Y02P60/87—Re-use of by-products of food processing for fodder production

Abstract

The invention belongs to the technical field of feed additives, and particularly provides a bacterium enzyme acid compound preparation, which comprises the following components in parts by weight: 3-4 parts of glucose oxidase, 3-4 parts of bacillus coagulans, 1-2 parts of lactic acid preparation and 0-2 parts of carrier. The bacterial enzyme acid composite preparation provided by the invention can obviously inhibit the growth and the propagation of fusarium, aspergillus ochraceus and penicillium citrinum, can inhibit the fusarium, aspergillus ochraceus and penicillium citrinum from the source by adding the bacterial enzyme acid composite preparation as an additive into feed, effectively removes the accumulation of corresponding enzyme toxin in the feed, can improve the safety of feed products and reduce public hazards.

Description

Bacterium enzyme acid composite preparation and application thereof
Technical Field
The invention belongs to the technical field of feed additives, and particularly relates to a bacterium enzyme acid compound preparation and application thereof.
Background
Common sources of mycotoxins in feed raw materials are: aspergillus (mainly secreting aflatoxin and the like), penicillium (mainly secreting ochratoxin, citrinin and the like), fusarium (mainly secreting vomitoxin, zearalenone, T-2 toxin and the like), fusarium, aspergillus ochraceus, penicillium (mainly secreting ergotoxin) and the like. The mass propagation of the mould can cause the feed to mildew, which not only reduces the palatability of the feed, but also influences the feed intake of livestock and poultry, and the growth and propagation of the mould are supplied by nutrient components in the feed, so the mildew can also reduce the nutrient value of the feed. In addition, mycotoxin is generated in the metabolic process of the mould fungi, and the mycotoxin not only affects the immune function of the livestock and poultry after the livestock and poultry eat the mycotoxin, but also causes the poisoning phenomenon of the livestock and poultry due to the high dose of the mycotoxin. According to statistics, more than 66% of feed raw materials are polluted by at least 1 mycotoxin, more than 2 mycotoxin exist in 35% of feed raw materials, the content of the mycotoxins such as corn meal, bean pulp and bran is 30-500 times of the content of whole grains, almost all peanut meal is polluted by the mycotoxins, and the feed mildew seriously harms the feed industry and the animal husbandry.
Therefore, development of green products capable of inhibiting harmful mold in feed raw materials is urgently needed, so that the safety of feed products is improved, and public hazards are reduced.
Disclosure of Invention
The invention aims to provide a feed additive capable of inhibiting growth and propagation of various moulds.
Therefore, the invention provides a bacterium enzyme acid compound preparation, which comprises the following components: 3-4 parts of glucose oxidase, 3-4 parts of bacillus coagulans, 1-2 parts of lactic acid preparation and 0-2 parts of carrier.
Specifically, the enzyme activity of the glucose oxidase is 8000U/g.
Specifically, the bacterial activity of Bacillus coagulans is 1.0 × 10 10 CFU/mL。
Specifically, the mass concentration of lactic acid in the lactic acid preparation is 25%.
The invention also provides a method for inhibiting the growth and the propagation of fusarium, aspergillus ochraceus and penicillium citrinum in the feed, which comprises the following steps: adding the bacterial enzyme acid composite preparation into physiological saline, extracting by a shaking table, centrifuging and taking supernate to obtain a bacterial enzyme acid composite preparation solution, and adding the bacterial enzyme acid composite preparation solution into feed to be mixed uniformly.
Specifically, the addition amount of the bacteria, enzyme and acid complexing agent solution is 0.48-1.6% by mass of the feed.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the bacterial enzyme acid composite preparation provided by the invention can obviously inhibit the growth and the propagation of fusarium, aspergillus ochraceus and penicillium citrinum, can inhibit the fusarium, aspergillus ochraceus and penicillium citrinum from the source by adding the bacterial enzyme acid composite preparation as an additive into feed, effectively removes the accumulation of corresponding enzyme toxin in the feed, can improve the safety of feed products and reduce public hazards.
The present invention will be described in further detail below with reference to the accompanying drawings.
Drawings
FIG. 1 shows the growth of Penicillium citrinum in different test groups in example 3 according to the present invention; a. test group 1; b. test group 2, c, test group 3; d. test group 4; e. test group 5; f. test group 6; g. a positive control; h. and (5) negative control.
FIG. 2 shows the growth of Penicillium citrinum in different control groups in example 3 of the present invention; a. control group 1; b. control 2, c, control 3.
FIG. 3 shows the growth of Aspergillus ochraceus in different groups according to example 3 of the invention; a. test group 1; b. test group 2, c, test group 3; d. test group 4; e. test group 5; f. test group 6; g. a positive control; h. and (5) negative control.
FIG. 4 shows growth of Aspergillus ochraceus in example 3 according to the invention; a. control group 1; b. control group 2, c, control group 3.
FIG. 5 shows the growth of Fusarium in different groups tested in example 3 of the present invention; a. test group 1; b. test groups 2, c, test group 3; d. test group 4; e. test group 5; f. test group 6; g. a positive control; h. and (5) negative control.
FIG. 6 shows the growth of Fusarium in example 3 of the present invention in different control groups; a. control group 1; b. control 2, c, control 3.
Detailed Description
The technical solutions in the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Although representative embodiments of the present invention have been described in detail, it will be understood by those skilled in the art that various modifications and changes may be made thereto without departing from the scope of the invention. Therefore, the scope of the present invention should not be limited to the embodiments, but should be defined by the appended claims and equivalents thereof.
The effect of the bacteria enzyme acid composite preparation of the present invention is examined by the following specific examples, and the lactic acid preparation used in the examples of the present invention has a lactic acid mass concentration of 25%.
Example 1:
the embodiment provides a compound preparation of an enzyme, which comprises: 3 parts of glucose oxidase, 4 parts of bacillus coagulans, 2 parts of lactic acid preparation and 2 parts of starch.
Wherein the enzyme activity of the glucose oxidase is 8000U/g, and the bacterial activity of the bacillus coagulans is 1.0 multiplied by 1010CFU/mL.
Example 2:
the embodiment provides a compound preparation of a fungal enzyme acid, which comprises the following components: 4 parts of glucose oxidase, 3 parts of bacillus coagulans, 1 part of lactic acid and 1 part of starch.
Wherein the enzyme activity of the glucose oxidase is 8000U/g, and the bacterial activity of the bacillus coagulans is 1.0 multiplied by 1010CFU/mL.
Example 3:
in the embodiment, the inhibition effect of the enzyme-acid compound preparation on fusarium, aspergillus ochraceus and penicillium citrinum is researched, and the specific steps are as follows.
1. Preparing culture medium
PDA culture medium: 200g of potatoes, 20g of glucose, 20g of agar and distilled water, and the balance is up to 1000mL. The liquid medium was not agar added.
A Chao's medium: : 3g of sodium nitrate, 1g of dipotassium hydrogen phosphate, magnesium sulfate (MgSO) 4 ·7H 2 O) 0.5g, potassium chloride 0.5g, ferrous sulfate 0.01g, sucrose 30g, agar 15-20 g, distilled water make up to 1000mL. The liquid medium was not supplemented with agar.
YPD medium: 1% of yeast extract, 2% of peptone, 2% of glucose and 2% of agar powder. The liquid medium was not supplemented with agar.
2. Preparation of experiment reagent
Preparing a mycolic acid compound preparation solution: weighing 1g of the enzyme-acid complex preparation provided in example 1, adding 99mL of sterilized normal saline, extracting for 30min in a shaking table, centrifuging to obtain supernatant, and diluting with sterilized normal saline to different concentrations for later use.
Preparing fusarium suspension: inoculating fusarium into 50mL PDA liquid culture medium, culturing at 28 ℃ for about 72h, observing the turbidity of the bacterial suspension, and selecting logarithmic phase bacterial liquid as test bacterial suspension for later use.
Preparation of ochratoxin suspension: inoculating aspergillus ochraceus into 50mL of a Chao's liquid culture medium, culturing at 28 ℃ for about 72 hours, observing the turbidity of the bacterial suspension, and selecting logarithmic phase bacterial liquid as the test bacterial suspension for later use.
Preparing a penicillium citrinum suspension: inoculating Penicillium citrinum to YPD slant, culturing at 28 deg.C for about 72 hr, and eluting bacterial solution from the slant with sterile normal saline to obtain a suspension.
Preparing a glucose oxidase solution: weighing 1g of glucose oxidase with enzyme activity of 8000U/g, adding 99mL of sterilized normal saline, extracting for 30min in a shaking table, centrifuging to obtain supernatant, and diluting with sterilized normal saline to different concentrations for later use.
Preparation of a bacillus coagulans solution: weighing 1g of Bacillus coagulans with bacterial activity of 1.0 × 1010CFU/mL, adding 99mL of sterilized normal saline, extracting for 30min in a shaking table, centrifuging to obtain supernatant, and diluting with sterilized normal saline to different concentrations for later use.
Preparing a lactic acid solution: weighing 1g of lactic acid, adding 99mL of sterilized normal saline, extracting for 30min in a shaking table, centrifuging to obtain supernatant, and diluting with sterilized normal saline to different concentrations for later use.
3. Plate mixed bacteria experiment
(1) Penicillium citrinum group
Taking 11 PDA plate culture media as a negative control group, a positive control group, a test group 1-6 and a control group 1-3 respectively, wherein the addition amount of the bacterial enzyme acid complex preparation solution and the penicillium citrinum suspension in each plate is as follows, wherein the concentration of the bacterial enzyme acid complex preparation is relative to the volume concentration of the culture media in the plate.
Positive control: adding 1mL of penicillium citrinum suspension;
negative control: adding 1mL of boiled and inactivated penicillium citrinum suspension for 5 minutes;
test group 1: adding 1mL of penicillium citrinum suspension and 0.08 percent of bacteria enzyme acid composite preparation solution;
test group 2: adding 1mL of penicillium citrinum suspension and 0.16% of bacterial enzyme acid composite preparation solution;
test group 3: adding 1mL of penicillium citrinum suspension and 0.32% of bacterial enzyme acid composite preparation solution;
test group 4: adding 1mL of penicillium citrinum suspension and 0.48% of bacterial enzyme acid composite preparation solution;
test group 5: adding 1mL of penicillium citrinum suspension and 0.64% of bacterial enzyme acid composite preparation solution;
test group 6: adding 1mL of penicillium citrinum suspension and 1.6% of bacterial enzyme acid composite preparation solution;
control group 1: adding 1mL of penicillium citrinum suspension and 1.6% of glucose oxidase solution;
control group 2: adding 1mL of penicillium citrinum suspension and 1.6% of bacillus coagulans solution;
control group 3: 1mL of Penicillium citrinum suspension and 1.6% lactic acid solution were added.
Each group was subjected to shake culture at 28 ℃ for 72 hours, and the growth of Penicillium citrinum was observed, as shown in FIGS. 1-2. No normal growth was observed on the 6 test Penicillium citrinum plates, but there were bubbles around the mycelia added to the plates, which may not kill or completely inhibit the mycelia, but the mycelia still had activity, but grew very slowly and could not reproduce. The positive control group and the control groups 1-4 have vigorous growth of penicillium citrinum.
The penicillium citrinum CGMCC 3.10140 bacteriostasis rate of each test group and each control group is detected, and the results are shown in Table 1.
TABLE 1 detection results of antibacterial rate of penicillium citrinum
Rate of inhibition of bacteria
Test group 1 90％
Test group 2 92％
Test group 3 97％
Test group 4 99％
Test group 5 100％
Test group 6 100％
Control group 1 30％
Control group 2 15％
Control group 3 10％
(2) Aspergillus ochraceus group
11 chaudou plate culture media are taken as a negative control group, a positive control group, a test group 1-6 and a control group 1-3 respectively, and the addition amount of the bacteria enzyme acid compound preparation solution and the ochratoxin suspension in each plate is as follows, wherein the concentration of the bacteria enzyme acid compound preparation is relative to the volume concentration of the culture media in the plate.
Positive control: 1mL of ochratoxin suspension is added;
negative control: adding 1mL of ochratoxin suspension inactivated by boiling for 5 minutes;
test group 1: adding 1mL of ochratoxin suspension and 0.08% of mycolic acid compound preparation solution;
test group 2: adding 1mL of ochratoxin suspension and 0.16% of mycolic acid compound preparation solution;
test group 3: adding 1mL of ochratoxin suspension and 0.32% of mycolic acid compound preparation solution;
test group 4: adding 1mL of ochratoxin suspension and 0.48% of mycolic acid compound preparation solution;
test group 5: adding 1mL of ochratoxin suspension and 0.64% of mycolic acid compound preparation solution;
test group 6: adding 1mL of ochratoxin suspension and 1.6% of mycolic acid compound preparation solution;
control group 1: adding 1mL of ochratoxin suspension and 1.6% glucose oxidase solution;
control group 2: 1mL of Aspergillus ochraceus suspension and 1.6% of Bacillus coagulans solution are added;
control group 3: 1mL of Aspergillus ochraceus suspension and 1.6% lactic acid solution were added.
Each group was subjected to shake culture at 28 ℃ for 72 hours, and the growth of Aspergillus ochraceus was observed, as shown in FIGS. 3-4. No growth of aspergillus ochraceus is observed on any of the 6 test groups, and the growth of aspergillus ochraceus is consistent with negative control, which shows that the inhibition effect of the mycophenolic acid product on aspergillus ochraceus is obvious. Positive control group and control groups 1-4 showed vigorous growth of aspergillus ochraceus.
The bacteriostasis rate of aspergillus ochraceus CGMCC 3.6255 of each test group and control group is detected, and the results are shown in Table 2.
TABLE 2 results of Aspergillus ochraceus bacteriostasis rate detection
Figure BDA0003763082500000061
Figure BDA0003763082500000071
(3) Fusarium group
11 YPD plate culture media are taken as a negative control group, a positive control group, a test group 1-6 and a control group 1-3 respectively, and the addition amount of the mycolic acid complex preparation solution and the fusarium suspension in each plate is as follows, wherein the concentration of the mycolic acid complex preparation is relative to the volume concentration of the culture media in the plate.
Positive control: adding 1mL of fusarium suspension;
negative control: adding 1mL of fusarium suspension inactivated by boiling for 5 minutes;
test group 1: adding 1mL of fusarium suspension and 0.08% of mycolic acid composite preparation solution;
test group 2: adding 1mL fusarium suspension and 0.16% mycolic acid composite preparation solution;
test group 3: adding 1mL of fusarium suspension and 0.32% of mycolic acid composite preparation solution;
test group 4: adding 1mL of fusarium suspension and 0.48% of mycolic acid composite preparation solution;
test group 5: adding 1mL of fusarium suspension and 0.64% of mycolic acid composite preparation solution;
test group 6: adding 1mL of fusarium suspension and 1.6% of mycolic acid composite preparation solution;
control group 1: adding 1mL of fusarium suspension and 1.6% of glucose oxidase solution;
control group 2: adding 1mL of fusarium suspension and 1.6% of bacillus coagulans solution;
control group 3: add 1mL of fusarium suspension and 1.6% lactic acid solution.
Each group was subjected to shake culture at 28 ℃ for 72 hours, and the growth of Fusarium was observed, as shown in FIGS. 5-6. The test group 1-3 with the addition amount of 0.08% -0.32% has the fusarium inhibition rate of more than 50%, and the test group 4-6 with the addition amount of 0.48% -1.6% can completely inhibit fusarium. The positive control group and the control groups 1-4 have vigorous growth of penicillium citrinum. Fusarium CGMCC 3.4610 bacteriostasis rate of each test group and control group is detected, and the results are shown in Table 3.
TABLE 3 detection results of the bacteriostatic rate of fusarium
Figure BDA0003763082500000072
Figure BDA0003763082500000081
In conclusion, when the addition amount is not less than 0.48%, the bacterial enzyme acid composite preparation has obvious growth inhibition effect on fusarium, aspergillus ochraceus and penicillium citrinum.
The above examples are merely illustrative of the present invention and should not be construed as limiting the scope of the invention, which is intended to be covered by the claims and any design similar or equivalent to the scope of the invention.

Claims (7)

1. A composite bacterial enzyme acid preparation, which is characterized by comprising the following components: 3-4 parts of glucose oxidase, 3-4 parts of bacillus coagulans, 1-2 parts of lactic acid preparation and 0-2 parts of carrier.
2. The mycolic acid complex formulation of claim 1, wherein: the enzyme activity of the glucose oxidase is 8000U/g.
3. The mycolic acid complex formulation of claim 1, wherein: the bacterial activity of the bacillus coagulans is 1.0 multiplied by 10 10 CFU/mL。
4. The mycolic acid complex formulation of claim 1, wherein: the mass concentration of lactic acid in the lactic acid preparation is 25%.
5. The use of a mycolic acid complex formulation of claims 1-4 in inhibiting the growth and reproduction of fusarium, aspergillus ochraceus, and penicillium citrinum.
6. A method for inhibiting the growth and the propagation of fusarium, aspergillus ochraceus and penicillium citrinum in feed is characterized by comprising the following steps: adding the mycolic acid complex preparation of any one of claims 1-4 into normal saline, extracting by a shaking table, centrifuging and taking supernatant to obtain a mycolic acid complex preparation solution, and adding the mycolic acid complex preparation solution into feed for uniformly mixing.
7. The method for inhibiting growth and reproduction of fusarium, aspergillus ochraceus and penicillium citrinum in the feed according to claim 6, wherein the method comprises the following steps: based on the mass of the feed, the addition amount of the bacteria, enzyme and acid complexing agent solution is 0.48-1.6%.
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CN107772195A (en) * 2016-08-27 2018-03-09 瑞普（天津）生物药业有限公司 One kind poultry poultry fodder takes off mould dose and preparation method thereof
CN107927328A (en) * 2017-04-26 2018-04-20 山东弘德农牧科技有限公司 A kind of preparation method and applications of feed composite microecologic agent
CN109221893A (en) * 2018-09-26 2019-01-18 山西大禹生物工程股份有限公司 Feed biology takes off mould dose and preparation method thereof
KR20210067850A (en) * 2019-11-29 2021-06-08 재단법인 발효미생물산업진흥원 Feed additive for preventing diarrhea of calf comprising mixture of Bacillus subtilis, saccharide and mineral as effective component and uses thereof
CN113575757A (en) * 2021-07-30 2021-11-02 上海长沪农业科技发展有限公司 Preparation method and application of composite microecological preparation for feed
CN114451486A (en) * 2022-01-21 2022-05-10 江西好实沃生物技术有限公司 Fungus enzyme compound feed additive and preparation method and application thereof

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