CN115372603A - Thrombus elastogram analyzer and using method thereof - Google Patents
Thrombus elastogram analyzer and using method thereof Download PDFInfo
- Publication number
- CN115372603A CN115372603A CN202210854535.XA CN202210854535A CN115372603A CN 115372603 A CN115372603 A CN 115372603A CN 202210854535 A CN202210854535 A CN 202210854535A CN 115372603 A CN115372603 A CN 115372603A
- Authority
- CN
- China
- Prior art keywords
- module
- blood
- sample
- reagent card
- shaking
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000000034 method Methods 0.000 title claims abstract description 26
- 208000007536 Thrombosis Diseases 0.000 title claims abstract description 14
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 123
- 239000008280 blood Substances 0.000 claims abstract description 78
- 210000004369 blood Anatomy 0.000 claims abstract description 78
- 238000001514 detection method Methods 0.000 claims abstract description 55
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 239000000523 sample Substances 0.000 claims description 156
- 230000007246 mechanism Effects 0.000 claims description 53
- 239000002699 waste material Substances 0.000 claims description 16
- 238000005259 measurement Methods 0.000 claims description 15
- 239000000725 suspension Substances 0.000 claims description 10
- 230000009471 action Effects 0.000 claims description 5
- 239000000758 substrate Substances 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 4
- 230000008569 process Effects 0.000 abstract description 10
- 238000012546 transfer Methods 0.000 description 10
- 230000015271 coagulation Effects 0.000 description 9
- 238000005345 coagulation Methods 0.000 description 9
- 230000023555 blood coagulation Effects 0.000 description 5
- 238000007689 inspection Methods 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000020764 fibrinolysis Effects 0.000 description 3
- 108010049003 Fibrinogen Proteins 0.000 description 2
- 102000008946 Fibrinogen Human genes 0.000 description 2
- 208000032843 Hemorrhage Diseases 0.000 description 2
- 208000034158 bleeding Diseases 0.000 description 2
- 230000000740 bleeding effect Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 229940012952 fibrinogen Drugs 0.000 description 2
- PGOHTUIFYSHAQG-LJSDBVFPSA-N (2S)-6-amino-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-4-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-5-amino-2-[[(2S)-2-[[(2S)-2-[[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-5-amino-2-[[(2S)-1-[(2S,3R)-2-[[(2S)-2-[[(2S)-2-[[(2R)-2-[[(2S)-2-[[(2S)-2-[[2-[[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-1-[(2S)-2-[[(2S)-2-[[(2S)-2-[[(2S)-2-amino-4-methylsulfanylbutanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]propanoyl]pyrrolidine-2-carbonyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]-4-methylpentanoyl]amino]acetyl]amino]-3-hydroxypropanoyl]amino]-4-methylpentanoyl]amino]-3-sulfanylpropanoyl]amino]-4-methylsulfanylbutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-hydroxybutanoyl]pyrrolidine-2-carbonyl]amino]-5-oxopentanoyl]amino]-3-hydroxypropanoyl]amino]-3-hydroxypropanoyl]amino]-3-(1H-imidazol-5-yl)propanoyl]amino]-4-methylpentanoyl]amino]-3-hydroxybutanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-5-carbamimidamidopentanoyl]amino]-5-oxopentanoyl]amino]-3-hydroxybutanoyl]amino]-3-hydroxypropanoyl]amino]-3-carboxypropanoyl]amino]-3-hydroxypropanoyl]amino]-5-oxopentanoyl]amino]-5-oxopentanoyl]amino]-3-phenylpropanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-methylbutanoyl]amino]-4-methylpentanoyl]amino]-4-oxobutanoyl]amino]-5-carbamimidamidopentanoyl]amino]-3-(1H-indol-3-yl)propanoyl]amino]-4-carboxybutanoyl]amino]-5-oxopentanoyl]amino]hexanoic acid Chemical compound CSCC[C@H](N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H]([C@@H](C)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1cnc[nH]1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCCN)C(O)=O PGOHTUIFYSHAQG-LJSDBVFPSA-N 0.000 description 1
- 108010094028 Prothrombin Proteins 0.000 description 1
- 102100027378 Prothrombin Human genes 0.000 description 1
- 108090000190 Thrombin Proteins 0.000 description 1
- 108010000499 Thromboplastin Proteins 0.000 description 1
- 102000002262 Thromboplastin Human genes 0.000 description 1
- 230000002159 abnormal effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000003511 endothelial effect Effects 0.000 description 1
- 238000009533 lab test Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229940039716 prothrombin Drugs 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 229960004072 thrombin Drugs 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/483—Physical analysis of biological material
- G01N33/487—Physical analysis of biological material of liquid biological material
- G01N33/49—Blood
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Physics & Mathematics (AREA)
- Food Science & Technology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Ecology (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention relates to a thromboelastogram analyzer and a using method thereof, wherein the thromboelastogram analyzer comprises a rack, a sample module, a shaking module, a sample tube grabbing module, a detection module, a reagent card bin, a reagent card grabbing module and a liquid transferring module; the shaking module is arranged on the rack and used for shaking blood in the sample tube, and the sample tube grabbing module is arranged on the rack and used for grabbing the sample tube on the sample module or the sample tube on the shaking module; the detection module is provided with a reaction cup, and the reaction cup is used for placing the uniformly shaken blood sample; the detection module is used for detecting blood in the reaction cup and generating a thrombelastogram; the reagent card bin is arranged on the frame body and used for placing reagent cards; the reagent card grabbing module is used for moving the reagent card in the reagent card bin to the detection module so as to enable the reagent on the reagent card to react with blood; the liquid transferring module is used for transferring the blood in the sample tube after shaking up into the reaction cup. The thrombus elastogram analyzer performs automatic detection in the whole process, thereby greatly improving the detection efficiency.
Description
Technical Field
The invention relates to the technical field of medical instruments, in particular to a thromboelastogram analyzer and a using method thereof.
Background
The coagulation function of the human body is completed by the combined action of platelets, a coagulation system, a fibrinolysis system, a vascular endothelial system and the like. The bleeding problems are clinically associated with a variety of diseases, and 90% of them can be diagnosed by laboratory tests of blood coagulation. The conventional detection items at present are blood coagulation routine and thromboelastogram. The blood coagulation routine includes Prothrombin Time (PT), activated Partial Thromboplastin Time (APTT), thrombin Time (TT), fibrinogen (FIB), etc., and analysis is performed to check whether each part of the coagulation system is abnormal in function.
The thromboelastogram is used for detecting the change curve of the clot viscoelasticity strength along with time in the coagulation process of a blood clot, can comprehensively and dynamically observe the whole coagulation and fibrinolysis processes, can quickly and completely detect the whole processes from the beginning of the coagulation to the formation and the stabilization of the blood clot, and provides data on the properties and the dynamic aspects of the coagulation and the fibrinolysis. The two are combined clinically, and the coagulation routine and the thrombus elastogram project are detected simultaneously, so that the more comprehensive coagulation function of a patient is known, and the bleeding risk is avoided.
However, at present, four items of blood coagulation and thromboelastogram in hospitals are mainly detected by a semi-automatic thromboelastogram instrument, samples, reagents and liquid transfer in the process are all manually operated, human factors can be generated to influence the detection result or the operation of the instrument requires a manual operation instrument, the automation degree is not high, the labor cost is increased, and in addition, manual detection easily causes carelessness and results in low detection precision.
Disclosure of Invention
The invention aims to provide a full-automatic thrombus elastogram analyzer which saves labor and has higher precision and a using method thereof. According to an aspect of the present invention, there is provided a thromboelastogram analyzer comprising:
the rack comprises a rack body and a base plate arranged on the rack body;
the sample module is arranged on the substrate and is used for placing a plurality of sample tubes;
the shaking module is arranged on the rack and used for shaking the blood in the sample tube;
the sample tube grabbing module is arranged on the frame body and used for grabbing the sample tubes on the sample module or the sample tubes on the shaking module;
the detection module is provided with a reaction cup, and the reaction cup is used for placing the uniformly shaken blood sample and reagent; the detection module is used for detecting the blood in the reaction cup and generating a thrombelastogram;
the reagent card bin is arranged on the frame body and used for placing reagent cards;
the reagent card grabbing module is used for moving a reagent card in the reagent card bin to the detection module so as to enable a reagent on the reagent card to react with blood;
and the pipetting module is used for transferring the blood and the reacted reagent in the sample tube after shaking up into the reaction cup.
As an embodiment of the invention, the shaking module comprises a shaking component and a clamping hand arranged on the shaking component, and the clamping hand is used for clamping a sample tube; the shake-up component can shake up the blood in the sample tube placed on the gripper, or the shake-up component can shake up the sample module to shake up the blood in the sample tube on the sample module.
As an embodiment of the present invention, the detection module further comprises a reaction platform, the reagent card grasping module can move the reagent card to the reaction platform, and the pipetting module can extract the reagent on the reagent card from the reaction platform and transfer the reagent to the reaction cup.
As an embodiment of the invention, the thromboelastogram analyzer further comprises a TIP cabin, a plurality of TIP heads can be placed in the TIP cabin, and the pipetting module can pick up the TIP heads from the TIP cabin and transfer blood in a sample tube or reagents on a reagent card through the TIP heads.
As an embodiment of the present invention, the thromboelastogram analyzer further comprises a waste module for receiving a used TIP head and a reagent card, wherein the TIP head is transferred to the waste module by a pipetting module and the reagent card is transferred into the waste module by the reagent card grasping module.
As an embodiment of the present invention, the detection module includes a horizontal moving mechanism, a vertical moving mechanism, a swinging mechanism, a cup cover removing mechanism, a reaction cup, an elastogram cup cover, and an elasticity measurement module, the elasticity measurement module is fixed on the vertical moving mechanism and can move up and down with the vertical moving mechanism, the swinging mechanism and the cup cover removing mechanism are fixed on the horizontal moving mechanism and can move horizontally with the horizontal moving mechanism, the reaction cup is mounted on the swinging mechanism and can rotate back and forth with the swinging mechanism, and the elastogram measurement module is used for measuring elasticity of blood and can form a thrombogram.
As an embodiment of the invention, the elasticity measuring module comprises an elasticity measuring bracket, a suspension wire, a probe and an angle sensor, wherein the upper end of the suspension wire is fixed on the elasticity measuring bracket, the probe is fixed at the lower end of the suspension wire, the angle sensor is fixed on the probe, the elastogram cup cover can be installed on the probe and can be detached from the probe by the cup detaching mechanism, and the angle sensor can detect the rotation angle of the probe and output the thromboelastogram.
The invention also provides a use method of the thromboelastogram analyzer, which is based on the thromboelastogram analyzer and comprises the following steps:
the sample tube grabbing module picks up a sample tube in the sample module;
the shaking module shakes the blood in the sample tube uniformly;
the reagent card grabbing module grabs the reagent card from the reagent card bin and places the reagent card in a reaction table of the detection module;
the liquid transferring module picks up the TIP head from the TIP bin, sucks blood from the sample tube after shaking up, adds the blood into a reaction cup on the detection module, and discards the TIP head;
the liquid transferring module loads a TIP head from the TIP bin for sampling the reagent on the reagent card and transplanting the reagent card into a reaction cup, and then discards the TIP head; the elasticity measuring module detects the blood elasticity in the reaction cup and outputs a thrombelastogram.
As an embodiment of the present invention, the shaking module shaking the blood in the sample tube includes:
shaking up the blood in the sample tube on the clamping hand by the shaking-up component; or
The sample block is shaken by a shake-up assembly to shake up the blood in the sample tube on the sample block.
As an embodiment of the invention, the detecting the blood elasticity in the reaction cup and outputting the thrombus elasticity map by the elasticity measuring module comprises:
moving the reaction cup to the lower part of the elastic force measuring module through a horizontal moving mechanism;
moving the elasticity measuring module downwards, and inserting the elasticity chart cup cover into the reaction cup;
the swinging mechanism starts to swing to drive the elastogram reaction cup to rotate back and forth, and the elastogram cup cover and the probe also rotate back and forth under the action of blood clots in the reaction cup;
the angle sensor detects the probe rotation angle and outputs a thromboelastogram.
The embodiment of the invention has the following beneficial effects:
in the thromboelastogram analyzer in the embodiment, the rack comprises a rack body and a base plate arranged on the rack body, and the sample module is arranged on the base plate and used for placing a plurality of sample tubes; the shaking module is arranged on the rack and used for shaking blood in the sample tube, and the sample tube grabbing module is arranged on the rack and used for grabbing the sample tube on the sample module or the sample tube on the shaking module; the detection module is provided with a reaction cup, and the reaction cup is used for placing the uniformly shaken blood sample; the detection module is used for detecting the blood in the reaction cup and generating a thrombelastogram; the reagent card bin is arranged on the frame body and used for placing reagent cards; the reagent card grabbing module is used for moving the reagent card in the reagent card bin to the detection module so as to enable the reagent on the reagent card to react with blood; the liquid transferring module is used for transferring the blood in the sample tube after shaking up into the reaction cup. The thrombelastogram analyzer in this embodiment, whole testing process need not artifically, and full automation detects to improved detection efficiency greatly, owing to be equipment automated inspection, do not have the artifical error that appears easily, it is also higher to detect the precision.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic diagram of a thrombelastogram analyzer according to an embodiment of the present invention;
FIG. 2 is a schematic diagram of the detection module shown in FIG. 1;
FIG. 3 is a flow chart of a method of using a thromboelastography analyzer in accordance with an embodiment of the present invention;
fig. 4 is a flowchart of step S160 in fig. 3;
wherein: 100. a frame; 110. a frame body; 120. a substrate; 130. a base plate; 140. a left support plate; 150. a right support plate; 200. a sample module; 300. shaking up the module; 400. a sample tube gripping module; 500. a detection module; 510. a horizontal movement mechanism; 520. a vertical moving mechanism; 530. a swing mechanism; 540. a cup cover removing mechanism; 550. a reaction cup; 560. an elasticity measuring module; 561. an elasticity measurement mount; 562. a suspension wire; 563. a probe; 564. an angle sensor; 600. a reagent card bin; 700. a reagent card capture module; 800. a pipetting module; 900. TIP storehouse; 1000. and (4) a waste module.
Detailed Description
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Preferred embodiments of the present invention are shown in the drawings. The invention may, however, be embodied in different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1-2, an embodiment of the invention provides a thromboelastogram analyzer, which is mainly used for detecting four blood coagulation items and thromboelastogram.
The thromboelastogram analyzer in this embodiment includes a rack 100, a sample module 200, a shake-up module 300, a sample tube grasping module 400, a detection module 500, a reagent card magazine 600, a reagent card grasping module 700, and a pipetting module 800.
The rack 100 includes a body 110 and a base plate 120 disposed on the body 110.
Further, the rack 100 further includes a bottom plate 130 disposed on the frame body 110, and a left support plate 140 and a right support plate 150 between the base plate 120 and the bottom plate 130. By arranging the base plate 120, the bottom plate 130, the left support plate 140, the right support plate 150 and the like on the rack body 110, the space for installing the sample module 200, the shaking module 300, the sample tube grabbing module 400, the detection module 500, the reagent card bin 600, the reagent card grabbing module 700, the pipetting module 800 and the like can be reasonably planned, so that the thromboelastogram analyzer can obtain smaller volume.
The sample module 200 is disposed on the substrate 120 and used for placing a plurality of sample tubes; by placing a plurality of sample tubes on the sample module 200, one sample tube does not need to be placed after detection, and the detection efficiency can be improved.
The shaking module 300 is disposed on the base plate 120 and used for shaking the blood in the sample tube. Blood in the appearance pipe need shake even before detecting through detection module 500, shake even process through shaking even module 300 and can realize blood automatically, shake even to required state through machinery, can be accurate shake even to can be so that the testing result is more accurate.
Further, the shaking module 300 comprises a shaking assembly (not shown in the figure) and a clamping hand (not shown in the figure) arranged on the shaking assembly, wherein the clamping hand is used for clamping the sample tube; the shake-up assembly can shake up blood in a sample tube placed on the gripper, or the shake-up assembly can shake up the sample module 200 to shake up blood in a sample tube on the sample module 200. In this embodiment, shaking may be performed for a plurality of detection states, for example, if only blood in some sample tubes on the sample module 200 needs to be detected, the sample tubes on the sample module 200 may be gripped and placed on the gripper by the sample tube gripping module 400, and the sample tubes on the gripper may be shaken only by the shaking assembly, so that the property of blood samples in sample tubes that are not detected yet in the future may not be affected. For another example, when blood on all sample tubes on the sample module 200 needs to be detected, the shaking module 300 can directly shake the sample module 200 to shake the blood in the sample tubes on the sample module 200, so that the blood in the sample tubes in the sample module 200 can be directly sucked by the pipetting module 800, steps of clamping and placing the sample tubes on the sample module 200 on the clamping hands and transferring the used sample tubes on the clamping hands back to the sample module 200 by the sample tube grabbing module 400 are reduced, and the detection efficiency is greatly improved.
The sample tube grasping module 400 is disposed on the rack body 110, and specifically, the sample tube grasping module 400 includes a robot and a moving platform, so as to change the position of the robot by the moving platform, and grasp the sample tube on the sample module 200 or the sample tube on the gripper by the robot.
The detection module 500 is arranged on the substrate 120, the reaction cup 550 is arranged on the detection module 500, and the reaction cup 550 is used for placing the uniformly shaken blood sample; the detection module 500 is used to detect blood in the reaction cup 550 and generate a thromboelastogram. Specifically, the detection module 500 detects a reaction after the blood in the reaction cup 550 is mixed with the reagent on the reagent card, thereby obtaining the thromboelastogram.
Further, the detection module 500 includes a horizontal moving mechanism 510, a vertical moving mechanism 520, a swinging mechanism 530, a cup cover removing mechanism 540, a reaction cup 550, an elastogram cup cover and an elasticity measurement module 560, wherein the elasticity measurement module 560 is fixed on the vertical moving mechanism 520 and can move up and down along with the vertical moving mechanism 520, the swinging mechanism 530 and the cup cover removing mechanism 540 are fixed on the horizontal moving mechanism 510 and can move horizontally along with the horizontal moving mechanism 510, the reaction cup 550 is installed on the swinging mechanism 530 and can rotate back and forth along with the swinging mechanism 530, the elastogram cup cover is arranged on the elasticity measurement module 560 and is used for being placed in the reaction cup 550 for rotating relative to the reaction cup 550, and thus the elastogram measurement module can measure the elasticity of blood and can form a thrombus elastogram.
Further, the elastic force measuring module 560 comprises an elastic force measuring support 561, a suspension wire 562, a probe 563, and an angle sensor 564, wherein the upper end of the suspension wire 562 is fixed on the elastic force measuring support 561, the probe 563 is fixed at the lower end of the suspension wire 562, the angle sensor 564 is fixed on the probe 563, an elastic force diagram cup cover can be mounted on the probe 563 and can be detached from the probe 563 by the cup detaching mechanism 540, and the angle sensor 564 can detect the rotation angle of the probe 563 and output a thromboelastogram. In this embodiment, when the detection is required, the reaction cup 550 is moved to the lower part of the elasticity measuring module 560 by the horizontal moving mechanism 510; then the elasticity measuring module 560 moves downwards, and the elasticity chart cup cover is inserted into the reaction cup 550; then the swinging mechanism 530 starts swinging to drive the elastogram reaction cup 550 to rotate back and forth, and the elastogram cup cover and the probe 563 also rotate back and forth under the action of the blood clot in the reaction cup 550; the angle sensor 564 thus detects the rotation angle of the probe 563 and outputs a thromboelastogram. Therefore, the detection is finished, and the elastic cup cover self-cup-cover-removing mechanism 540 is removed from the probe 563 after the detection is finished. Whole testing process is automated inspection, and detection efficiency is high, also makes a round trip to rotate under the effect of the blood clot in reaction cup 550 through elasticity picture bowl cover and probe 563, can improve the detection precision.
The reagent card grasping module 700 is disposed on the rack 110, and the reagent card grasping module 700 is configured to move the reagent card in the reagent card magazine 600 to the detection module 500 to react the reagent on the reagent card with blood.
Further, the detecting module 500 further includes a reaction platform (not shown), the reagent card grabbing module 700 can move the reagent card to the reaction platform, and the pipetting module 800 can take the reagent on the reagent card from the reaction platform and transfer the reagent to the reaction cup 550, so as to conveniently suck the reagent on the reagent card.
Of course, in some embodiments, the reagent card may be transferred to the reaction cup 550 for several seconds and then removed by the reagent card grasping module 700, and the reagent in the reagent card may also react with the blood in the reaction cup 550.
The pipetting module 800 is used to transfer the blood in the shaken well into the reaction cup 550.
Further, the pipetting module 800 is also used to aspirate and transfer reagents from the reagent card into the reaction cup 550.
Specifically, the pipetting module 800 includes a robot arm and a mobile station, so that the position of the robot arm can be changed by the mobile station, and blood in the sample tube and reagent on the reagent card can be extracted by the robot arm.
Further, the thromboelastogram analyzer further comprises a TIP bin 900, the TIP bin 900 is arranged on the left support plate 140, a plurality of TIP heads can be placed in the TIP bin 900, and the pipetting module 800 can pick up the TIP heads from the TIP bin 900 and transfer blood in the sample tubes or reagents on the reagent cards through the TIP heads. It should be noted that the blood in the sampling tube and the reagent on the reagent card need to be sucked through different TIPs.
Further, the thromboelastogram analyzer further comprises a waste module 1000, the waste module 1000 is disposed on the bottom plate 130, the waste module 1000 is used for receiving the used TIP head and the reagent card, wherein the TIP head is transferred to the waste module 1000 through the liquid transfer module 800, and the reagent card is transferred into the waste module 1000 through the reagent card grasping module 700. The used reagent card and TIP head can be transferred into the waste module 1000 through the waste module 1000, thereby facilitating recovery.
The thrombelastogram analyzer in this embodiment, whole testing process need not artifically, and full automation detects to improved detection efficiency greatly, owing to be equipment automated inspection, do not have the artifical error that appears easily, it is also higher to detect the precision.
Referring to fig. 1-4, the present invention further provides a method for using the thromboelastogram analyzer, the method comprising the following steps:
s110, the sample tube grabbing module 400 picks up sample tubes in the sample module 200;
s120, shaking the blood in the sample tube by the shaking module 300;
s130, the reagent card grabbing module 700 grabs the reagent card from the reagent card bin 600 and places the reagent card in a reaction platform of the detection module 500;
s140, the liquid transferring module 800 picks up the TIP head from the TIP bin 900, sucks blood from the sample tube after shaking up, adds the blood into the reaction cup 550 on the detection module 500, and discards the TIP head;
s150, loading a TIP head from the TIP bin 900 by the liquid transfer module 800, wherein the TIP head is used for sampling the reagent on the reagent card and transferring the reagent card to the reaction cup 550, and then discarding the TIP head;
s160, the elastic force measuring module 560 detects the elastic force of the blood in the cuvette 550 and outputs a thromboelastogram.
The use method of the thromboelastogram analyzer in the embodiment can automatically detect blood and output the thromboelastogram through the steps, so that the detection efficiency can be greatly improved.
In this embodiment, the sequence of steps S110 to S160 is not limited.
In one embodiment, the shake-up module 300 shakes up the blood in the sample tube includes:
shaking up the blood in the sample tube on the clamping hand by the shaking-up component; or
The sample block 200 is shaken by a shake assembly to shake up the blood in the sample tube on the sample block 200.
In a specific embodiment, S110 is before S120, which mainly corresponds to detecting blood in a portion of the sample tube on the sample module 200, and the sample tube is picked up in the sample module 200 by the sample tube grasping module 400, then the sample tube is moved to the hand grip of the shaking module 300, and then the blood in the sample tube on the hand grip is shaken by S120.
In another specific embodiment, S120 is before S110, and mainly for all the vials on the sample module 200 that need to be tested, the sample module 200 is shaken by the shaking module 300 to shake the blood in all the vials on the sample module 200 at a time, and then the vials are picked up in the sample module 200 by the vial grabbing module 400, and the blood in the vials on the vial grabbing module 400 is sucked by the pipetting module 800. Of course, the step S110 may be omitted, and the blood in the sample tube on the sample module 200 may be directly sucked by the pipette module 800. The detection efficiency is further improved.
In one embodiment, the elastic force measuring module 560 detects the elastic force of blood in the cuvette 550 and outputs the thromboelastogram including:
s161, moving the reaction cup 550 to the position below the elasticity measuring module 560 through the horizontal moving mechanism 510;
s162, moving the elasticity measuring module 560 downwards, and inserting the elasticity chart cup cover into the reaction cup 550;
s163 and the swinging mechanism 530 start swinging to drive the elastogram reaction cup 550 to rotate back and forth, and the elastogram cup cover and the probe 563 also rotate back and forth under the action of the blood clot in the reaction cup 550;
s164, the angle sensor 564 detects the rotation angle of the probe 563, and outputs a thromboelastogram.
By this step S160, the thrombelastogram can be automatically, rapidly, and accurately acquired.
Further, the use method of the thrombelastogram analyzer further comprises the following steps:
s170, after the measurement is finished, the cup cover removing mechanism 540 removes the cup cover of the elastic chart from the probe 563;
s180, the reagent card grabbing module 700 grabs the tested reagent card from the reaction table and throws the tested reagent card into the waste module 1000.
Through steps S170 and S180, waste generated during the inspection process can be automatically recycled.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the claims. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (10)
1. A thromboelastogram analyzer, comprising:
the rack comprises a rack body and a base plate arranged on the rack body;
the sample module is arranged on the substrate and is used for placing a plurality of sample tubes;
the shaking module is arranged on the rack and used for shaking the blood in the sample tube;
the sample tube grabbing module is arranged on the frame body and used for grabbing the sample tubes on the sample module or the sample tubes on the shaking module;
the detection module is provided with a reaction cup, and the reaction cup is used for placing the uniformly shaken blood sample and reagent; the detection module is used for detecting the blood in the reaction cup and generating a thrombelastogram;
the reagent card bin is arranged on the frame body and used for placing a reagent card;
the reagent card grabbing module is used for moving a reagent card in the reagent card bin to the detection module so as to enable a reagent on the reagent card to react with blood;
and the pipetting module is used for transferring the blood and the reacted reagent in the sample tube after shaking up into the reaction cup.
2. The thromboelastogram analyzer of claim 1, wherein the shake-up module comprises a shake-up assembly and a gripper disposed on the shake-up assembly, the gripper being configured to grip a sample tube; the shaking assembly can shake up blood in the sample tube placed on the gripper, or the shaking assembly can shake the sample module to shake up blood in the sample tube on the sample module.
3. The thromboelastogram analyzer of claim 2, wherein the detection module further comprises a reaction platform, the reagent card grasping module is capable of moving the reagent card onto the reaction platform, and the pipetting module is capable of extracting reagents on the reagent card from the reaction platform and transferring the reagents into the reaction cup.
4. The thromboelastogram analyzer of claim 1, further comprising a TIP compartment in which a plurality of TIP heads can be placed, the pipetting module being capable of picking up a TIP head from the TIP compartment and transferring reagents on a blood or reagent card in a sample tube through the TIP head.
5. The thromboelastogram analyzer of claim 4, further comprising a waste module for receiving spent TIP heads and reagent cards, wherein TIP heads are transferred to the waste module by a pipetting module and reagent cards are transferred into the waste module by the reagent card grasping module.
6. The thromboelastogram analyzer of claim 1, wherein the detection module comprises a horizontal moving mechanism, a vertical moving mechanism, a swinging mechanism, a cup cover removing mechanism, a reaction cup, an elastogram cup cover and an elasticity measurement module, the elasticity measurement module is fixed on the vertical moving mechanism and can move up and down along with the vertical moving mechanism, the swinging mechanism and the cup cover removing mechanism are fixed on the horizontal moving mechanism and can move horizontally along with the horizontal moving mechanism, the reaction cup is mounted on the swinging mechanism and can rotate back and forth along with the swinging mechanism, and the elastogram measurement module is used for measuring the elasticity of blood and can form the thromboelastogram.
7. The thromboelastogram analyzer of claim 6, wherein the elasticity measurement module comprises an elasticity measurement bracket, a suspension wire, a probe, an angle sensor, the upper end of the suspension wire is fixed on the elasticity measurement bracket, the probe is fixed on the lower end of the suspension wire, the angle sensor is fixed on the probe, the elastogram cover can be mounted on the probe and can be detached from the probe by the cup detaching mechanism, and the angle sensor can detect the rotation angle of the probe and output the thromboelastogram.
8. A method for using a thromboelastogram analyzer, the method being based on the thromboelastogram analyzer of claim 7, the method comprising:
the sample tube grabbing module picks up a sample tube in the sample module;
the shaking module shakes the blood in the sample tube uniformly;
the reagent card grabbing module grabs the reagent card from the reagent card bin and places the reagent card in a reaction platform of the detection module;
the liquid transferring module picks up the TIP head from the TIP bin, sucks blood from the sample tube after shaking up, adds the blood into a reaction cup on the detection module, and discards the TIP head;
the liquid transferring module loads a TIP head from the TIP bin for sampling the reagent on the reagent card and transplanting the reagent card into a reaction cup, and then discards the TIP head; the elasticity measuring module detects the blood elasticity in the reaction cup and outputs a thrombelastogram.
9. The method of using a thromboelastography analyzer of claim 8, wherein the shaking module shaking the blood in the sample tube comprises:
shaking up the blood in the sample tube on the clamping hand by the shaking-up component; or
The sample block is shaken by a shake-up assembly to shake up the blood in the sample tube on the sample block.
10. The method for using the thromboelastogram analyzer according to claim 9, wherein the elastic force measuring module detecting the elastic force of blood in the reaction cup and outputting the thromboelastogram comprises:
moving the reaction cup to the lower part of the elastic force measuring module through a horizontal moving mechanism;
moving the elasticity measuring module downwards, and inserting the elasticity chart cup cover into the reaction cup;
the swinging mechanism starts to swing to drive the elastogram reaction cup to rotate back and forth, and the elastogram cup cover and the probe also rotate back and forth under the action of blood clots in the reaction cup;
the angle sensor detects the probe rotation angle and outputs a thromboelastogram.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210854535.XA CN115372603A (en) | 2022-07-15 | 2022-07-15 | Thrombus elastogram analyzer and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210854535.XA CN115372603A (en) | 2022-07-15 | 2022-07-15 | Thrombus elastogram analyzer and using method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115372603A true CN115372603A (en) | 2022-11-22 |
Family
ID=84062639
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210854535.XA Pending CN115372603A (en) | 2022-07-15 | 2022-07-15 | Thrombus elastogram analyzer and using method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115372603A (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103837694A (en) * | 2014-03-20 | 2014-06-04 | 南通市伊士生物技术有限责任公司 | Full-automatic two-channel quantitative detector |
| CN113219188A (en) * | 2021-04-25 | 2021-08-06 | 深圳沃德生命科技有限公司 | Integrated modular structure and equipment for thrombus elasticity and blood coagulation analysis |
| CN113791235A (en) * | 2021-11-18 | 2021-12-14 | 深圳优迪生物技术有限公司 | Full-automatic thromboelastogram instrument and blood sample testing method |
| CN113985043A (en) * | 2021-10-27 | 2022-01-28 | 赫菲斯科技(深圳)有限公司 | Thrombelastogram instrument |
| CN114062703A (en) * | 2021-11-09 | 2022-02-18 | 赫菲斯科技(深圳)有限公司 | Thrombelastogram detection module and detection method thereof |
-
2022
- 2022-07-15 CN CN202210854535.XA patent/CN115372603A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103837694A (en) * | 2014-03-20 | 2014-06-04 | 南通市伊士生物技术有限责任公司 | Full-automatic two-channel quantitative detector |
| CN113219188A (en) * | 2021-04-25 | 2021-08-06 | 深圳沃德生命科技有限公司 | Integrated modular structure and equipment for thrombus elasticity and blood coagulation analysis |
| CN113985043A (en) * | 2021-10-27 | 2022-01-28 | 赫菲斯科技(深圳)有限公司 | Thrombelastogram instrument |
| CN114062703A (en) * | 2021-11-09 | 2022-02-18 | 赫菲斯科技(深圳)有限公司 | Thrombelastogram detection module and detection method thereof |
| CN113791235A (en) * | 2021-11-18 | 2021-12-14 | 深圳优迪生物技术有限公司 | Full-automatic thromboelastogram instrument and blood sample testing method |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN110398596B (en) | Full-automatic sample injection blood cell analysis and measurement method and device and method for uniformly mixing peripheral blood test tubes | |
| JP2009515140A (en) | How to process chemical and coagulation samples in a laboratory work cell | |
| EP3101428B1 (en) | Automatic analytical apparatus | |
| US9915675B2 (en) | Methods and apparatus for determining aspiration and/or dispensing volume and/or pipette positioning | |
| CN110291377B (en) | Sample preparation device | |
| KR101854704B1 (en) | Multi-sample automatic analyzing apparatus | |
| JPS58154662A (en) | Automatic analyzer | |
| US20140064019A1 (en) | Sample processing apparatus and sample processing method | |
| CN104730268A (en) | Method and device for detecting liquid samples | |
| JPH0989907A (en) | Automatic hemanalysis device | |
| CN114324930A (en) | Integrated blood detection and analysis system and method | |
| CN110927397A (en) | Sample analyzer, sample analysis method and storage medium | |
| JP5137969B2 (en) | Collection of liquid analysis samples for clinical analysis purposes | |
| CN115372603A (en) | Thrombus elastogram analyzer and using method thereof | |
| KR101770679B1 (en) | Method of in vitro automatic diagnostics | |
| CN114460320B (en) | Sample analyzer and sample detection process thereof | |
| US20140011290A1 (en) | Collection of liquid analytical samples for clinical analytical purpose and device thereof | |
| JPS63269061A (en) | Liquid specimen portionwise sampling method of automatic chemical analyser | |
| EP3896460A1 (en) | Automatic sampling method for handling whole blood | |
| CN219424244U (en) | Test tube mixing and moving mechanism | |
| CN113960301B (en) | Full-automatic thrombus elastogram detection device | |
| CN110967505A (en) | Sample retesting method and sample analysis device | |
| CN114545008B (en) | Sample analyzer and manual sample feeding assembly thereof | |
| CN114460321A (en) | Sample analyzer and test tube detection assembly thereof | |
| CN115704825A (en) | Sample test control method, sample analysis apparatus, and storage medium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20221122 |
|
| RJ01 | Rejection of invention patent application after publication |