CN115364919A - Constant temperature amplification detection device - Google Patents
Constant temperature amplification detection device Download PDFInfo
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- CN115364919A CN115364919A CN202211306531.4A CN202211306531A CN115364919A CN 115364919 A CN115364919 A CN 115364919A CN 202211306531 A CN202211306531 A CN 202211306531A CN 115364919 A CN115364919 A CN 115364919A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J6/00—Heat treatments such as Calcining; Fusing ; Pyrolysis
- B01J6/005—Fusing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/18—Means for temperature control
- B01L2300/1855—Means for temperature control using phase changes in a medium
Abstract
The embodiment of the application belongs to the field of medical detection, and relates to a constant-temperature amplification detection device which comprises a temperature control platform, a reaction platform, a plurality of first heat-conducting pieces and a plurality of second heat-conducting pieces; the temperature control platform is provided with a phase change temperature control cavity, and the phase change temperature control cavity is used for internally arranging a phase change material; the reaction platform is connected with the temperature control platform, and the reaction platform is provided with at least one reaction cavity for containing a liquid to be reacted; each first heat conduction piece and each second heat conduction piece are arranged in the phase-change temperature control cavity, and the first heat conduction pieces are in heat conduction connection with the heat source and used for conducting heat of the heat source to the phase-change temperature control cavity; the second heat conducting piece is connected with the reaction platform and used for conducting heat of the phase-change material in the phase-change temperature control cavity to the reaction cavity. The application provides a technical scheme can effectively promote heat conduction efficiency.
Description
Technical Field
The application relates to the technical field of medical detection, in particular to a constant-temperature amplification detection device.
Background
In the current constant temperature amplification detection device, a heating platform is in surface contact connection with a temperature control platform; in practical application, the heat source in the heating platform produces heat, and in the phase transition accuse temperature chamber on with heat conduction to accuse temperature platform through the contact surface of heating platform and accuse temperature platform, so that phase transition material melts in the phase transition accuse temperature chamber, but behind the phase transition accuse temperature chamber of heat source heat conduction to accuse temperature platform on the heating platform, only phase transition accuse temperature chamber is close to the one side on the heating platform and is heated, the temperature difference that leads to phase transition accuse temperature intracavity to be close to and keep away from these two parts of heating platform is big, the phase transition material of phase transition accuse temperature intracavity is difficult to the effective melting that obtains, heat conduction efficiency is poor, and then lead to phase transition accuse temperature chamber heat-conduction to the reaction chamber temperature instability on the reaction platform, accuse temperature ability is poor.
Disclosure of Invention
The embodiment of the application provides a constant-temperature amplification detection device, which is used for solving the problem of poor heat conduction efficiency in the prior art.
In order to solve the above technical problems, an embodiment of the present invention provides an isothermal amplification detection apparatus, which adopts the following technical solutions:
comprises a temperature control platform, a reaction platform, a plurality of first heat-conducting pieces and a plurality of second heat-conducting pieces;
the temperature control platform is provided with a phase change temperature control cavity, and the phase change temperature control cavity is used for internally arranging a phase change material; the reaction platform is connected with the temperature control platform, and is provided with at least one reaction cavity for containing a liquid to be reacted;
each first heat-conducting piece and each second heat-conducting piece are arranged in the phase-change temperature control cavity; the first heat conducting piece is in heat conduction connection with a heat source and is used for conducting heat of the heat source to the phase-change temperature control cavity; the second heat conducting piece is connected with the reaction platform and used for conducting heat of the phase-change material in the phase-change temperature control cavity to the reaction cavity.
Furthermore, the constant-temperature amplification detection device also comprises a heating platform, the heating platform is used for accommodating the heat source, and the first heat conduction piece is used for being in heat conduction connection with the heat source in the heating platform;
the temperature control platform is arranged on the heating platform in an overlapping manner; or, the heating platform is provided with a first mounting hole, and the temperature control platform is arranged in the first mounting hole.
Furthermore, a first mounting hole is formed in the heating platform, and the temperature control platform is mounted in the first mounting hole; the temperature control platform is provided with a mounting seat positioned in the phase-change temperature control cavity, the mounting seat is provided with a second mounting hole, and the reaction platform is arranged in the second mounting hole;
the second heat conducting piece is arranged on one surface, close to the phase change temperature control cavity, of the mounting seat.
Further, the reaction platform and the temperature control platform can be detachably connected, or the reaction platform and the heating platform can be detachably connected.
Further, the heat source used for accommodating the heating platform is a chemical self-heating source.
Further, at least one second heat-conducting member is arranged between two adjacent first heat-conducting members.
Furthermore, the first heat-conducting piece and the second heat-conducting piece are vertically arranged at an interval, and the vertical interval distance L between the first heat-conducting piece and the second heat-conducting piece is greater than or equal to 0mm and less than or equal to 10mm.
Further, the first heat conduction member and/or the second heat conduction member extend along the length direction of the phase change temperature control cavity.
Further, the first heat conducting member and/or the second heat conducting member are heat conducting fins or heat conducting silica gel.
Further, the isothermal amplification detection device further comprises a flow channel platform arranged on the reaction platform;
the runner platform is provided with at least one first infusion runner, and one reaction cavity is correspondingly communicated with the at least one first infusion runner.
Furthermore, the constant-temperature amplification detection device also comprises an infusion platform arranged on the flow passage platform;
the transfusion platform is provided with at least one liquid cavity, one liquid cavity is correspondingly communicated with at least one reaction cavity, and each liquid cavity is provided with a phase change valve; and a third heat-conducting piece is arranged on one surface, close to the flow channel platform, of the infusion platform, one end of the third heat-conducting piece is in heat-conducting connection with the phase change valve, and the other end of the third heat-conducting piece extends into the reaction cavity.
Furthermore, the reaction platform is provided with at least two positioning parts;
and at least two positioning parts and the reaction platform are enclosed to form a positioning area for installing the runner platform and/or the infusion platform.
Further, the isothermal amplification detection device further comprises at least one microfluidic platform;
the microfluidic platform is detachably mounted on the side face of the reaction platform, at least one second infusion flow channel is formed in the microfluidic platform, and one second infusion flow channel is correspondingly communicated with at least one first infusion flow channel.
Furthermore, at least one side surface of the reaction platform is provided with a first connecting part, and at least one side surface of the microfluidic platform is provided with a second connecting part; the first connecting part and the second connecting part are matched for use, so that the reaction platform and the microfluidic platform can be detachably connected.
Compared with the prior art, the embodiment of the application mainly has the following beneficial effects: the phase-change temperature control cavity is internally provided with a plurality of first heat-conducting pieces, so that after heat of a heat source is conducted to the first heat-conducting pieces, the heat of the heat source is dissipated to each position in the phase-change temperature control cavity through the first heat-conducting pieces, the coverage range is wide, the phase-change material at each position in the phase-change temperature control cavity can be fully heated, the heat conduction uniformity is stronger, and the melting speed of the phase-change material is effectively improved; meanwhile, when the phase-change material in the phase-change temperature control cavity is converted into a liquid state from a solid state by heat conduction of the first heat conduction piece, the liquid phase-change material fills a gap between the first heat conduction piece and the second heat conduction piece to form a heat conduction path of the first heat conduction piece, the phase-change material and the second heat conduction piece, so that after the liquid phase-change material conducts heat to the second heat conduction piece, the second heat conduction piece conducts the heat to the reaction cavity for heating and detecting a liquid to be reacted in the reaction cavity; in addition, when liquid phase-change material is behind conducting the heat to second heat-conducting piece, phase-change material changes solid state from liquid into, when first heat-conducting piece conducts the heat to solid phase-change material once more, solid phase-change material absorbs the heat once more and melts, makes each first heat-conducting piece, phase-change material and each second heat-conducting piece three form and lasts stable heat-conduction like this, effectively promotes the stability of heat-conduction efficiency and reaction intracavity temperature to the realization is to the stable accuse temperature of reaction intracavity temperature.
Drawings
In order to illustrate the present application or prior art more clearly, a brief description of the drawings needed for the description of the embodiments or prior art will be given below, it being clear that the drawings in the following description are some embodiments of the present application and that other drawings can be derived from them by a person skilled in the art without inventive effort.
FIG. 1 is a schematic perspective view of a hidden microfluidic platform according to an embodiment of the isothermal amplification and detection device of the present application;
FIG. 2 is an exploded view of a hidden microfluidic platform according to an embodiment of the isothermal amplification and detection device of the present invention;
FIG. 3 is a schematic cross-sectional view of a hidden microfluidic platform according to an embodiment of the isothermal amplification and detection device of the present invention;
FIG. 4 is a schematic perspective view of a first embodiment of the isothermal amplification and detection device according to the present invention;
FIG. 5 is a schematic perspective view of the microfluidic platform of FIG. 4;
FIG. 6 is an exploded view of a microfluidic platform according to a second embodiment of the isothermal amplification and detection device of the present invention.
Reference numerals are as follows:
100. a temperature control platform; 110. a phase change temperature control cavity; 120. a mounting seat; 121. a second mounting hole; 200. a reaction platform; 210. a reaction chamber; 220. a positioning part; 221. positioning the clamping boss; 222. positioning the side plate; 223. a guide surface; 230. positioning an interval; 240. a first connection portion; 250. connecting the side plates; 260. a third connecting portion; 300. a first heat-conducting member; 400. a second heat-conducting member; 500. a heating platform; 510. a first mounting hole; 520. a cavity; 530. an installation opening; 540. an operation port; 550. a fourth connecting portion; 600. a flow channel platform; 610. a first infusion flow channel; 700. a transfusion platform; 710. a liquid chamber; 720. a phase change valve; 730. a third heat-conducting member; 800. a microfluidic platform; 810. a second infusion flow channel; 820. a second connecting portion.
Detailed Description
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs; the terminology used in the description of the application herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application; the terms "including" and "having," and any variations thereof in the description and claims of this application and the description of the figures above, are intended to cover non-exclusive inclusions. The terms "first," "second," and the like in the description and claims of this application or in the above-described drawings are used for distinguishing between different objects and not for describing a particular order.
Reference herein to "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment can be included in at least one embodiment of the application. The appearances of the phrase in various places in the specification are not necessarily all referring to the same embodiment, nor are separate or alternative embodiments mutually exclusive of other embodiments. It is explicitly and implicitly understood by one skilled in the art that the embodiments described herein can be combined with other embodiments.
The first embodiment is as follows:
referring to fig. 1 to 3, an isothermal amplification detection apparatus according to an embodiment of the present disclosure includes a temperature control platform 100, a reaction platform 200, a plurality of first heat-conducting members 300, and a plurality of second heat-conducting members 400.
In some embodiments, referring to fig. 1 and 3, the temperature control platform 100 is provided with a phase-change temperature control chamber 110, the phase-change temperature control chamber 110 is used for accommodating a phase-change material, the phase-change material is used for absorbing heat of a heat source and releasing the absorbed heat, and the temperature control heating of the reaction chamber 210 is realized through the cooperation of the first heat conduction member 300 and the second heat conduction member 400. In practical application, the phase change material corresponding to the phase change point can be selected according to the target heating temperature of the solution to be reacted, for example, when the target heating temperature of the solution to be reacted is RPA (recombinase polymerase amplification) at 35-42 ℃, the phase change material with the phase change point at 43 ℃ can be selected, and when the target heating temperature of the solution to be reacted is LAMP (loop-mediated isothermal amplification)/HDA (helicase-dependent amplification) at 60-70 ℃, the phase change material with the phase change point at 70 ℃ can be selected.
Further, the phase change material is a solid-liquid phase change material; in practical application, when a heat source is heated to the melting temperature of the phase-change material, the phase-change material generates phase change from a solid state to a liquid state, and the phase-change material absorbs and stores a large amount of latent heat in the melting process; when the phase change material cools, the stored heat is dissipated to the environment within a certain temperature range, and reverse phase change from liquid to solid is carried out. In both phase change processes, the energy stored or released is called the latent heat of phase change.
In some embodiments, referring to fig. 2 and fig. 3, the reaction platform 200 is disposed on the temperature control platform 100, and the reaction platform 200 is provided with a reaction cavity 210, where the reaction cavity 210 is used for accommodating a liquid to be reacted and is used for heating and reacting the liquid to be reacted, so as to implement detection of the liquid to be reacted. Further, the number of above-mentioned reaction chamber 210 is at least one, so can be according to the detection demand of difference, choose for use and have the reaction platform 200 that corresponds reaction chamber 210 quantity with the detection demand, promote this application constant temperature and amplify detection device's suitability.
In some embodiments, referring to fig. 2 and 3, each of the first heat-conducting members 300 and each of the second heat-conducting members 400 are disposed in the phase-change temperature-control chamber 110; the first heat conducting member 300 is used for being in heat conduction connection with a heat source, so as to conduct heat of the heat source into the phase-change temperature control cavity 110; the second heat-conducting member 400 is connected to the reaction platform 200 for conducting heat of the phase-change material in the phase-change temperature control chamber 110 to the reaction chamber 210.
The phase-change temperature control cavity 110 is internally provided with a plurality of first heat conducting pieces 300, so that after heat of a heat source is conducted to the first heat conducting pieces 300, the heat of the heat source is dissipated to each position in the phase-change temperature control cavity 110 through the first heat conducting pieces 300, the coverage range is wide, the phase-change material at each position in the phase-change temperature control cavity 110 can be fully heated, the heat conduction uniformity is stronger, and the melting speed of the phase-change material is effectively improved; meanwhile, when the phase-change material in the phase-change temperature control cavity 110 is transformed from a solid state to a liquid state by heat conduction of the first heat-conducting member 300, the liquid phase-change material fills a gap between the first heat-conducting member 300 and the second heat-conducting member 400 to form a heat conduction path of the first heat-conducting member 300, the phase-change material and the second heat-conducting member 400, so that after the liquid phase-change material conducts the heat to the second heat-conducting member 400, the second heat-conducting member 400 conducts the heat to the reaction cavity 210 for heating and detecting the liquid to be reacted in the reaction cavity 210; in addition, after the liquid phase-change material transfers heat to the second heat-conducting member 400, the phase-change material is transformed from a liquid state to a solid state, and when the first heat-conducting member 300 transfers heat to the solid phase-change material again, the solid phase-change material absorbs heat again and melts, so that the first heat-conducting members 300, the phase-change material and the second heat-conducting members 400 form continuous and stable heat conduction, the heat conduction efficiency and the stability of the temperature in the reaction chamber 210 are effectively improved, and the stable temperature control of the temperature in the reaction chamber 210 is realized.
In some embodiments, referring to fig. 2 and 3, the isothermal amplification test device further comprises a heating platform 500, wherein the heating platform 500 is configured to accommodate the heat source, and the first heat conduction member 300 is configured to be in heat conduction connection with the heat source in the heating platform 500; the temperature control platform 100 is stacked on the heating platform 500. In practical application, the heat source generates heat, and the heat generated by the heat source is transferred to the first heat conducting member 300 in the phase-change temperature-control cavity 110 by using the heating platform 500 and the temperature-control platform 100 as heat conducting media, and the temperature-control platform 100 and the heating platform 500 are mounted in an overlapping manner, so that the contact area between the temperature-control platform 100 and the heating platform 500 is large, and the heat conduction efficiency is high.
In some embodiments, referring to fig. 2 and 3, the heating platform 500 is provided with a cavity 520 for accommodating a heat source and a mounting opening 530 communicated with the cavity 520, and the temperature control platform 100 is stacked on the mounting opening 530, so that when the heat source generates heat, the heat can be directly conducted to the temperature control platform 100, thereby reducing thermal resistance in the heat conduction process and effectively improving heat conduction efficiency.
Furthermore, the heat source is a chemical self-heating source, and compared with the existing electric heating mode, the chemical self-heating source does not depend on a power line and a battery, can be adapted to various use scenes such as outdoors and indoors, and improves the universality of the constant-temperature amplification detection device.
Further, in some embodiments, the self-heating chemical source is an iron self-heating substance (such as iron powder), which is oxidized after contacting with air to generate heat for heating the phase-change temperature-control chamber 110; in other embodiments, the chemical self-heat source is a supersaturated solution self-heat substance and a metal sheet, and after the supersaturated solution self-heat substance is stimulated by the metal member (for example, knocking the metal member), the metastable state of the supersaturated solution self-heat substance loses balance, excessive solute is precipitated, i.e., a cooling crystallization process is performed, in which the supersaturated solution self-heat substance releases heat and becomes a stable solid, so as to heat the phase-change temperature control cavity 110. The self-heating material of iron or supersaturated solution is adopted, the temperature of heat generated after self-heating is about 60 ℃, and compared with the traditional method that the temperature of heat generated after self-heating by a self-heating material of quicklime is more than 90 ℃, the safety is higher.
Further, referring to fig. 1 to 3, the heating platform 500 is provided with an operation opening 540, and the operation opening 540 is used for an operator to operate the chemical self-heating source to trigger the chemical self-heating source to release heat; if the chemical self-heating source is an iron self-heating substance (such as iron powder), a shield (such as a sealing tape) is disposed at the operation opening 540, and an operator removes the shield to communicate the cavity 520 with the outside atmosphere, so that outside air enters the cavity 520 and reacts with the iron self-heating substance to generate heat, thereby heating the phase-change temperature control cavity 110; if the chemical self-heating source is a supersaturated solution self-heating substance, the operation port 540 may be sealed or unsealed, and an operator may knock the metal piece in the accommodating cavity 520 through the operation port 540, so that the supersaturated solution is crystallized to generate heat, and the phase-change temperature control cavity 110 is heated.
In some embodiments, referring to fig. 2 and 3, the reaction platform 200 is detachably connected to the temperature control platform 100, or the reaction platform 200 is detachably connected to the heating platform 500. The detachable connection mode can be changed according to different requirements, such as changing the reaction platform 200, adapting to the reaction detection of different liquids to be reacted by changing the reaction platform 200 with different specifications of the reaction chamber 210, or adjusting the heat conduction efficiency of the second heat conduction member 400 by changing the reaction platform 200 with different specifications of the second heat conduction member 400, so as to achieve the purpose of controlling the temperature in the reaction chamber 210; if the temperature control platform 100 is replaced, the temperature control platform 100 with the phase-change temperature control cavities 110 of different specifications is replaced to adjust the filling amount of the phase-change material in the temperature control platform 100, so as to adjust the heat conduction efficiency, or the temperature control platform 100 with the first heat conduction members 300 of different specifications is replaced to adjust the heat conduction efficiency of the first heat conduction members 300, so as to achieve the purpose of controlling the temperature in the reaction cavity 210.
In some embodiments, referring to fig. 2 and 3, the reaction platform 200 is detachably connected to the heating platform 500 by a detachable structure, wherein the detachable structure comprises a connection side plate 250, a third connection portion 260 and a fourth connection portion 550, the connection side plate 250 is disposed at a side of the reaction platform 200, the third connection portion 260 is disposed on the connection side plate 250, the fourth connection portion 550 is disposed on the heating platform 500, and the third connection portion 260 and the fourth connection portion 550 are detachably connected; in practical applications, the third connection portion 260 and the fourth connection portion 550 are used together to detachably connect the reaction platform 200 and the heating platform 500; further, the reaction platform 200 and the temperature control platform 100 or the heating platform 500 may be detachably connected by a fastening manner, an inserting manner, a screw connection, a mortise and tenon connection, and the like, which is not limited herein.
In some embodiments, referring to fig. 2 and 3, at least one second heat-conducting member 400 is disposed between two adjacent first heat-conducting members 300. So make first heat-conducting member 300 and second heat-conducting member 400 form the dislocation interlock mode, use the vertical face of first heat-conducting member 300 and the vertical face of second heat-conducting member 400 as heat-conducting medium respectively, heat-conducting area is big, effectively promotes the heat-conduction efficiency of the two.
In an exemplary embodiment, the second heat-conducting members 400 and the first heat-conducting members 300 are disposed in a staggered manner, and after the reaction platform 200 is connected to the temperature control platform 100, the second heat-conducting members 400 and the first heat-conducting members 300 are engaged in a staggered manner, so as to ensure the heat-conducting area of each second heat-conducting member 400 and each first heat-conducting member 300, and further improve the heat-conducting efficiency of the two members.
Further, after the first heat conduction member 300 and the second heat conduction member 400 are disposed in a staggered manner, as the vertical distance between the surface of the first heat conduction member 300 close to the reaction platform 200 and the surface of the second heat conduction member 400 close to the phase change temperature control chamber 110 is larger, the heat conduction area between the first heat conduction member 300 and the second heat conduction member 400 is larger, and the heat conduction efficiency of the phase change material between the first heat conduction member 300 and the second heat conduction member 400 is higher.
In some embodiments, the first heat-conducting member 300 and the second heat-conducting member 400 are vertically spaced, and the vertical spacing distance L between the first heat-conducting member 300 and the second heat-conducting member 400 is 0mm ≦ L ≦ 10mm; along with the vertical distance between the first heat conduction member 300 and the second heat conduction member 400 is reduced, the phase-change material heat conduction efficiency of the first heat conduction member 300 and the second heat conduction member 400 is enhanced, and by adopting the value range of the vertical distance, the heat conduction efficiency of the phase-change materials in the first heat conduction member 300, the second heat conduction member 400 and the phase-change temperature control cavity 110 is ensured while the structural compactness of the formed constant-temperature amplification detection device is ensured.
It should be noted that the vertical distance is a distance in a vertical direction, which is a direction perpendicular to the reaction platform 200.
Further, in the present embodiment, the first heat-conducting member 300 and the second heat-conducting member 400 can be disposed in a staggered manner, so that the second heat-conducting member 400 can be influenced by heat conduction between two adjacent first heat-conducting members 300, thereby effectively improving heat conduction efficiency; the first heat conduction member 300 and the second heat conduction member 400 may be disposed opposite to each other, so as to facilitate the manufacturing process of the first heat conduction member 300 and the second heat conduction member 400.
In some embodiments, referring to fig. 2 and 3, the first heat-conducting member 300 and/or the second heat-conducting member 400 extend along the length direction of the phase-change temperature-control chamber 110. Therefore, the first heat conduction member 300 and/or the second heat conduction member 400 have a larger surface area, and after the phase-change material in the phase-change temperature control chamber 110 is converted from a solid state to a liquid state and fills the gap between the first heat conduction member 300 and the second heat conduction member 400, the contact area between the first heat conduction member 300 and/or the second heat conduction member 400 and the liquid phase-change material is larger, so that the heat conduction efficiency among the first heat conduction member 300, the phase-change material and the second heat conduction member 400 is effectively improved.
In some embodiments, referring to fig. 2 and 3, the first and/or second heat-conducting members 300, 400 are heat-conducting fins; the heat conduction fins are in a sheet shape, the sheet-shaped heat conduction fins have larger surface area, the contact area between the heat conduction fins and the phase change material is increased, if the first heat conduction piece 300 is a heat conduction fin, the heat conduction area covered by the first heat conduction piece 300 is wide, the contact area between the first heat conduction piece 300 and the phase change material in the phase change temperature control cavity 110 is large, and the efficiency of converting the phase change material from a solid state to a liquid state is increased; similarly, if the second heat conducting member 400 is a heat conducting fin, the heat conducting efficiency between the second heat conducting member 400 and the liquid phase-change material is effectively improved by the sheet-shaped heat conducting fin, so as to stably control the temperature in the reaction chamber 210. In another embodiment, the first heat-conducting member 300 and/or the second heat-conducting member 400 is a heat-conducting silicone; in the heat conduction of the first heat-conducting member 300, the phase-change material and the second heat-conducting member 400, the heat-conducting silica gel can utilize its own high heat-conducting property to improve the heat-conducting efficiency among the first heat-conducting member 300, the phase-change material and the second heat-conducting member 400, thereby realizing the stable temperature control of the temperature in the reaction chamber 210.
In some embodiments, referring to fig. 2 and 3, the isothermal amplification test device further comprises a flow channel platform 600 disposed on the reaction platform 200; the channel platform 600 is provided with at least one first infusion channel 610, and one reaction chamber 210 is correspondingly communicated with at least one first infusion channel 610.
The first infusion flow channel 610 is used for conveying an external solution to be reacted into the reaction chamber 210; in some embodiments, one reaction chamber 210 is correspondingly communicated with one first liquid conveying flow channel 610, so as to convey the liquid to be reacted into the reaction chamber 210 through the first liquid conveying flow channel 610; in other embodiments, one reaction chamber 210 is correspondingly communicated with at least two first liquid conveying flow channels 610, and the liquids to be reacted in each first liquid conveying flow channel 610 can be respectively conveyed into the reaction chamber 210 and mixed in the reaction chamber 210, so as to meet different use requirements.
In some embodiments, referring to fig. 2 and 3, the isothermal amplification test device further comprises an infusion platform 700 disposed on the flow channel platform 600;
the infusion platform 700 is provided with at least one liquid cavity 710, one liquid cavity 710 is correspondingly communicated with at least one reaction cavity 210, and each liquid cavity 710 is provided with a phase change valve 720; a third heat-conducting member 730 is disposed on a surface of the infusion platform 700 close to the flow channel platform 600, one end of the third heat-conducting member 730 is connected to the phase change valve 720 in a heat-conducting manner, and the other end of the third heat-conducting member 730 extends into the reaction chamber 210.
Initially, the phase change material in phase change valve 720 is in a solid state, in a state where phase change valve 720 is closed; after the heat in the reaction chamber 210 is conducted to the phase change valve 720 through the third heat conducting member 730, the phase change material of the phase change valve 720 is heated, and after the temperature of the phase change valve 720 reaches the phase change melting point, the phase change material filled in the phase change valve 720 is converted from the solid state to the liquid state, so that the phase change valve 720 is switched from the closed state to the open state, the liquid to be reacted in the liquid chamber 710 can enter the reaction chamber 210 through the phase change valve 720 for reaction, and the phase change material converted from the solid state to the liquid state enters the reaction chamber 210 along with the liquid to be reacted, so that the use is convenient and fast compared with the traditional method of manually opening or closing an infusion valve to realize the liquid to be reacted.
In some embodiments, referring to fig. 2 and 3, the reaction platform 200 is provided with at least two positioning portions 220; at least two positioning parts 220 and the reaction platform 200 enclose a positioning area 230 for installing the runner platform 600 and/or the infusion platform 700. The positioning section 230 is used for positioning and installing the runner platform 600 and/or the infusion platform 700, so as to improve the accuracy and stability of the installation position of the runner platform 600 and/or the infusion platform 700, and also improve the installation convenience of the runner platform 600 and/or the infusion platform 700.
In the following, the horizontal direction is a direction parallel to the reaction platform 200, and the vertical direction is a direction perpendicular to the reaction platform 200.
Further, referring to fig. 2 and 3, each positioning portion 220 includes a positioning protrusion 221 and a positioning side plate 222, the positioning side plates 222 are installed on one side of the reaction platform 200 away from the temperature control platform 100, the positioning protrusion 221 is installed on the inner side of the positioning side plate 222, and the positioning side plates 222, the positioning protrusions 221 and one side of the reaction platform 200 away from the temperature control platform 100 are enclosed to form the positioning area 230; the installation position of the flow channel platform 600 and/or the infusion platform 700 in the horizontal direction is limited by the at least two positioning side plates 222, and the height of the flow channel platform 600 and/or the infusion platform 700 in the vertical direction is limited by the positioning clamping protrusions 221, so that positioning installation on the flow channel platform 600 and/or the infusion platform 700 is formed, and the accuracy and the stability of the installation position of the flow channel platform 600 and/or the infusion platform 700 are improved.
For example, the number of the positioning portions is two, the positioning side plates 222 of the two positioning portions are respectively and oppositely disposed on two sides of the reaction platform 200 to respectively position two sides of the flow channel platform 600 and/or the infusion platform 700, and accordingly, the positioning locking protrusion 221 is disposed on each positioning side plate 222, so that two positions of the flow channel platform 600 and/or the infusion platform 700 in the horizontal direction and a previous position in the vertical direction can be positioned, and the accuracy and stability of the installation position of the flow channel platform and/or the infusion platform 700 are high.
Further, referring to fig. 2 and 3, the number of the positioning protrusions 221 is at least two, so that after the flow channel platform 600 and/or the infusion platform 700 are positioned and installed in the positioning section 230, the at least two positioning protrusions 221 can fix a plurality of positions in the vertical direction of the flow channel platform 600 and/or the infusion platform 700, so as to further improve the stability of the flow channel platform 600 and/or the infusion platform 700.
Further, referring to fig. 2 and 3, a guide surface 223 is disposed on a surface of the positioning clip protrusion 221 away from the reaction platform 200, and the guide surface 223 plays a role of guiding, so that the flow channel platform 600 and/or the infusion platform 700 can be guided and installed in the positioning area 230, thereby improving the assembly efficiency. In some embodiments, the guiding surface 223 is an inclined surface, and the length of the positioning protrusion 221 is gradually increased in a direction that the positioning protrusion 221 approaches the reaction platform 200, so that the inclined surface serves to guide and mount the flow channel platform 600 and/or the infusion platform 700, and at the same time, the side of the positioning protrusion 221 that approaches the reaction platform 200 can clamp the flow channel platform 600 and/or the infusion platform 700.
In some embodiments, referring to fig. 2-5, the isothermal amplification detection device further comprises at least one microfluidic platform 800;
the micro-fluidic platform 800 is detachably mounted on the side surface of the reaction platform 200, the micro-fluidic platform 800 is provided with at least one second infusion flow channel 810, and one second infusion flow channel 810 is correspondingly communicated with at least one first infusion flow channel 610.
The second infusion channel 810 is used for delivering a solution to be reacted into the reaction chamber 210; in practical application, as the microfluidic platform 800 is detachably connected with the reaction platform 200, the microfluidic platform 800 with the corresponding number of second infusion flow channels 810 can be selected according to different use requirements, so that the applicability is wide; in an example, one second infusion flow channel 810 is correspondingly communicated with two first infusion flow channels 610, so that the to-be-reacted liquid can be detected in different reaction chambers 210 after being shunted and respectively flowing into the corresponding first infusion flow channels 610, so as to meet different use requirements.
In some embodiments, at least one side of the reaction platform 200 described with reference to fig. 2 to 5 is provided with a first connection part 240, and at least one side of the microfluidic platform 800 is provided with a second connection part 820; the first connection portion 240 and the second connection portion 820 are used together to detachably connect the reaction platform 200 and the microfluidic platform 800.
Thus, the reaction platform 200 may have one side or at least two sides connected with the microfluidic platform 800; when one of the side surfaces of the reaction platform 200 is provided with the first connection part 240, the microfluidic platform 800 and the reaction platform 200 are connected from the side surface; when at least two side surfaces of the reaction platform 200 are provided with the first connecting parts 240, the fluidic platform can be mounted on at least two side surfaces, for example, when at least two microfluidic platforms 800 are mounted on the reaction platform 200 at the same time, the liquids to be reacted of at least two microfluidic platforms 800 can be mixed and then enter the same reaction chamber 210, or the liquids to be reacted of at least two microfluidic platforms 800 can enter different reaction chambers 210, so as to meet different use requirements.
In an example, the reaction platform 200 is square, the square reaction platform 200 has four side surfaces, and the first connecting portion 240 is disposed on at least one side surface of the reaction platform 200, so that the reaction platforms 200 corresponding to the number and the arrangement positions of the first connecting portions 240 can be selected according to different use requirements, and the versatility is high.
Meanwhile, since at least one side surface of the microfluidic platform 800 is provided with the second connection part 820, when one side surface of the microfluidic platform 800 is provided with the second connection part 820, the microfluidic platform 800 and the reaction platform 200 are connected from the side; when the second connection parts 820 are arranged on at least two side surfaces of the micro-fluidic platform 800, the second connection parts 820 on each side surface of the micro-fluidic platform 800 can be used for being connected with the first connection parts 240 on the reaction platforms 200, namely, the micro-fluidic platform 800 can be connected with at least one reaction platform 200, the liquid to be reacted is conveyed to at least one reaction platform 200 by shunting through the second transfusion flow channel 810 on the micro-fluidic platform 800, and meanwhile, the micro-fluidic platform 800 and the reaction platforms 200 can be connected from multiple directions, so that different use requirements are met, and the universality is strong.
In some embodiments, the reaction platform 200 and the microfluidic platform 800 are detachably connected by a fastening manner, and the first connection portion 240 and the second connection portion 820 may be the two fastening portions or the fastening portion and the fastening groove, and the fastening connection manner is easy to assemble and disassemble and has strong fastening stability; in another embodiment, the reaction platform 200 and the microfluidic platform 800 may be detachably connected by means of a plug-in connection, a screw connection, a mortise-and-tenon connection, and the like, which is not limited herein.
In some embodiments, the isothermal amplification detection device of the present application is used for nucleic acid detection, and at this time, the heating platform 500 may adopt a chemical self-heat source to adapt to different detection scenes indoors and outdoors, and the first heat-conducting member 300, the second heat-conducting member 400 and the phase-change material in the phase-change temperature control cavity 110 are used in cooperation, so that the three form a heat conduction system, thereby improving the heat conduction efficiency of the three, improving the temperature control performance in the reaction cavity 210, and further effectively improving the accuracy of nucleic acid detection.
Example two:
referring to fig. 6, a second embodiment of the present application provides an isothermal amplification detection apparatus, which is different from the first embodiment in that: the heating platform 500 is provided with a first mounting hole 510, and the temperature control platform 100 is installed in the first mounting hole 510.
In the process of heat conduction of the heat source built in the heating platform 500, each inner wall surface of the first mounting hole 510 can be used as a medium for heat conduction of the temperature control platform 100 and the heating platform 500 to form surrounding type heat conduction, so that the heat conduction efficiency of the temperature control platform 100 and the heating platform 500 can be further improved; and the first heat conduction member 300 and the second heat conduction member 400 in the phase change temperature control cavity 110 of the temperature control platform 100 are matched, so that the heat of the heat source on the heating platform 500 can be quickly conducted to the first heat conduction member 300, and due to the design of the first mounting hole 510, the layout of the first heat conduction member 300 and the second heat conduction member 400 can be more concentrated, the heat conduction efficiency of the first heat conduction member 300, the phase change material and the second heat conduction member 400 is faster, and the situation that the temperature of the reaction cavity 210 is unstable is further effectively avoided.
Further, the first mounting hole 510 is a long hole, and the design of the long hole can increase the contact area between the temperature control platform 100 located in the long hole and the inner wall surface of the long hole, thereby increasing the heat conduction efficiency of the two. In some embodiments, the long holes are circular long holes, and the shape of the temperature control platform 100 corresponds to the circular long holes, so that the convenience of installing the temperature control platform 100 to the circular long holes is facilitated, and the assembly efficiency is improved; in other embodiments, the long hole is a square long hole, and the shape of the temperature control platform 100 corresponds to the square long hole, so that the corners of the square long hole are used to improve the stability of the temperature control platform 100 after being installed in the square long hole.
Further, after controlling temperature platform 100 and installing to first mounting hole 510, the one side that accuse temperature platform 100 is close to reaction platform 200 and the one side parallel and level that heating platform 500 is close to reaction platform 200 so in order to guarantee the integrative nature of the constant temperature amplification detection device of this application, and promote the protectiveness to accuse temperature platform 100.
In some embodiments, the temperature-controlled stage 100 is provided with a mounting base 120 located in the phase-change temperature-controlled chamber 110, the mounting base 120 is provided with a second mounting hole 121, and the reaction stage 200 is mounted in the second mounting hole 121;
the second heat conduction member 400 is installed on a surface of the mounting base 120 close to the phase-change temperature-control chamber 110.
In practical applications, each inner wall surface of the second mounting hole 121 can be used as a medium for heat conduction between the second heat conducting member 400 and the reaction platform 200 to form a surrounding heat conduction, so as to further improve the heat conduction efficiency between the temperature controlled platform 100 and the heating platform 500.
Further, the second mounting hole 121 is a long hole, and the design of the long hole can increase the contact area between the reaction platform 200 located in the long hole and the inner wall surface of the long hole, thereby improving the heat conduction efficiency of the two.
It should be understood that the above-described embodiments are merely exemplary of some, and not all, embodiments of the present application, and that the drawings illustrate preferred embodiments of the present application without limiting the scope of the claims appended hereto. This application is capable of embodiments in many different forms and is provided for the purpose of enabling a thorough understanding of the disclosure of the application. Although the present application has been described in detail with reference to the foregoing embodiments, it will be apparent to one skilled in the art that the present application may be practiced without modification or with equivalents of some of the features described in the foregoing embodiments. All equivalent structures made by using the contents of the specification and the drawings of the present application are directly or indirectly applied to other related technical fields and are within the protection scope of the present application.
Claims (10)
1. A constant-temperature amplification detection device is characterized by comprising a temperature control platform, a reaction platform, a plurality of first heat-conducting pieces and a plurality of second heat-conducting pieces;
the temperature control platform is provided with a phase change temperature control cavity, and the phase change temperature control cavity is used for internally arranging a phase change material; the reaction platform is connected with the temperature control platform, and is provided with at least one reaction cavity for containing a liquid to be reacted;
each first heat-conducting piece and each second heat-conducting piece are arranged in the phase-change temperature control cavity; the first heat conducting piece is in heat conduction connection with a heat source and used for conducting heat of the heat source to the phase-change temperature control cavity; the second heat conducting piece is connected with the reaction platform and used for conducting heat of the phase-change material in the phase-change temperature control cavity to the reaction cavity.
2. The isothermal amplification detection device of claim 1, further comprising a heating platform for receiving the heat source, wherein the first heat conducting element is in heat-conducting connection with the heat source in the heating platform;
the temperature control platform is arranged on the heating platform in an overlapping manner; or, the heating platform is provided with a first mounting hole, and the temperature control platform is arranged in the first mounting hole.
3. The isothermal amplification detection device according to claim 2, wherein when the temperature control platform is mounted in the first mounting hole, the temperature control platform is provided with a mounting seat located in the phase-change temperature control cavity, the mounting seat is provided with a second mounting hole, and the reaction platform is mounted in the second mounting hole;
the second heat conducting piece is arranged on one surface, close to the phase change temperature control cavity, of the mounting seat.
4. The isothermal amplification detection device according to claim 2, wherein the reaction platform is detachably connected to the temperature control platform, or the reaction platform is detachably connected to the heating platform;
and/or the heat source used for accommodating the heating platform is a chemical self-heating source.
5. The isothermal amplification detection device according to any one of claims 1 to 4,
at least one second heat-conducting member is arranged between two adjacent first heat-conducting members;
and/or the first heat-conducting piece and the second heat-conducting piece are vertically arranged at intervals, and the vertical interval distance L between the first heat-conducting piece and the second heat-conducting piece is more than or equal to 0mm and less than or equal to 10mm;
and/or the first heat-conducting piece and/or the second heat-conducting piece extend along the length direction of the phase-change temperature control cavity;
and/or the first heat-conducting piece and/or the second heat-conducting piece are heat-conducting fins or heat-conducting silica gel.
6. The isothermal amplification detection device according to any one of claims 1 to 4, further comprising a flow channel platform disposed on the reaction platform;
the runner platform is provided with at least one first infusion runner, and one reaction cavity is correspondingly communicated with at least one first infusion runner.
7. The isothermal amplification detection device of claim 6, further comprising an infusion platform disposed on the flow platform;
the transfusion platform is provided with at least one liquid cavity, one liquid cavity is correspondingly communicated with at least one reaction cavity, and each liquid cavity is provided with a phase change valve; and a third heat-conducting piece is arranged on one surface, close to the flow channel platform, of the infusion platform, one end of the third heat-conducting piece is in heat-conducting connection with the phase change valve, and the other end of the third heat-conducting piece extends into the reaction cavity.
8. The isothermal amplification detection device according to claim 7, wherein the reaction platform is provided with at least two positioning portions;
and at least two positioning parts and the reaction platform are enclosed to form a positioning area for installing the runner platform and/or the infusion platform.
9. The isothermal amplification detection device of claim 6, further comprising at least one microfluidic platform;
the microfluidic platform is detachably mounted on the side face of the reaction platform, at least one second infusion flow channel is formed in the microfluidic platform, and one second infusion flow channel is correspondingly communicated with at least one first infusion flow channel.
10. The isothermal amplification detection device according to claim 9, wherein at least one side surface of the reaction platform is provided with a first connection portion, and at least one side surface of the microfluidic platform is provided with a second connection portion; the first connecting part and the second connecting part are matched for use, so that the reaction platform and the microfluidic platform can be detachably connected.
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