CN115363021A - Silver ion-containing antibacterial composition and preparation method thereof - Google Patents

Silver ion-containing antibacterial composition and preparation method thereof Download PDF

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CN115363021A
CN115363021A CN202111092640.6A CN202111092640A CN115363021A CN 115363021 A CN115363021 A CN 115363021A CN 202111092640 A CN202111092640 A CN 202111092640A CN 115363021 A CN115363021 A CN 115363021A
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silver
antibacterial
water
ion
silver ion
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周慕妍
周志才
周志茹
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A01N25/04—Dispersions, emulsions, suspoemulsions, suspension concentrates or gels
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/22—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing ingredients stabilising the active ingredients
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/16—Heavy metals; Compounds thereof
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/50—Isolated enzymes; Isolated proteins

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  • Health & Medical Sciences (AREA)
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  • Environmental Sciences (AREA)
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  • Agronomy & Crop Science (AREA)
  • Wood Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Toxicology (AREA)
  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Inorganic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
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  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention belongs to the technical field of antibacterial compositions, and particularly relates to a silver ion antibacterial composition and a preparation method thereof. A silver ion antibacterial composition is prepared from the following raw materials: electrolytic silver water, biomass polysaccharide, antibacterial peptide, an auxiliary agent and water. The mass concentration of the silver ion long-acting antibacterial composition prepared by the invention in electrolytic silver water is 5-30ppm, so that the long-acting antibacterial effect can be ensured, the accumulation of silver ions in the environment or human body is avoided, and the trace efficient antibacterial effect is realized; the silver ion long-acting antibacterial composition prepared by the application can prolong the antibacterial effect of a system and prolong the action of the antibacterial composition and a bacterial cell membrane under the synergistic action of the chitosan derivative and the antibacterial peptide.

Description

Silver ion-containing antibacterial composition and preparation method thereof
Technical Field
The invention belongs to the technical field of A01N antibacterial agents, and particularly relates to a silver ion long-acting antibacterial composition and a preparation method thereof.
Background
Inorganic compounds are used as the early antibacterial agents, and sulfur powder and copper preparations, among them, are still used. However, with the improvement of the quality of life by people with the progress of society, the antibacterial agents used in daily life are also changing significantly.
The antibacterial agents appearing in daily life of people include hypochlorous acid antibacterial agents, ethanol antibacterial agents and silver ion antibacterial agents, and the silver ion antibacterial agents strongly adsorb protease of bacterial bodies mainly through strong oxidizability and are rapidly combined with the protease to destroy the protease of the bacterial bodies, so that the bacteria cannot breathe, lose activity and die. For example, the Chinese patent with the application number of 202110320134.1 discloses a long-acting spectral activity nano silver ion disinfectant and a preparation method thereof, and the interaction between a silver ion solution and a stabilizing agent and a dispersing agent is utilized in the disclosed patent to ensure that the prepared disinfectant has long-acting, spectral and efficient sterilization effects.
However, in the patent disclosed, the disinfectant can achieve the purpose of antibiosis due to the action of the disinfectant and the mycoprotease, and with the mutation and development of bacteria, only one gene is changed to be enough to resist the disinfectant, so that the antibacterial disinfectant loses the effect.
Therefore, in view of the current situation, it is an important task to prepare an antibacterial composition having a better effect.
Disclosure of Invention
In order to solve the above problems, the present invention provides a silver ion long-acting antibacterial composition, which is prepared from the following raw materials: electrolyzed silver water, biomass polysaccharide, antibacterial peptide, auxiliary agent and water;
the biomass polysaccharide is at least one selected from sodium alginate, chitosan, carrageenan, gelatin, carboxymethyl cellulose and derivatives thereof.
In some preferred embodiments, the concentration of the electrolyzed silver water is 5-30ppm by mass.
In some preferred embodiments, the concentration of the electrolyzed silver water is 10-20ppm by mass.
In some preferred embodiments, the concentration of the electrolyzed silver water is 15ppm by mass.
In some preferred embodiments, the biomass polysaccharide is chitosan.
In some preferred embodiments, the biomass polysaccharide is selected from chitosan derivatives.
In some preferred embodiments, the chitosan is a modified chitosan.
In some preferred embodiments, the modified chitosan is carboxymethyl chitosan.
In some preferred embodiments, the degree of substitution of the carboxymethyl chitosan is 60 to 90%.
In some preferred embodiments, the degree of substitution of the carboxymethyl chitosan is 80%.
Carboxymethyl chitosan, cat # C832672, available from mclin reagent net.
In some preferred embodiments, the weight ratio of the electrolyzed silver water to the antimicrobial peptide is 8: (1-5).
In some preferred embodiments, the weight ratio of the electrolyzed silver water to the antimicrobial peptide is 8:2.5.
in some preferred embodiments, the antimicrobial peptide is selected from Human β -defensin-3.
Human β -defensin-3, CAS:221230-01-9, available from MedChemexpress LLC of the United states.
In some preferred embodiments, the auxiliary agent is selected from at least one of a dispersant, a stabilizer, a complexing agent, and a pH adjuster.
In some preferred embodiments, the stabilizer is selected from at least one of glucose and citric acid.
In some preferred embodiments, the complexing agent is selected from at least one of tetrasodium glutamate diacetate, trisodium methylglycine diacetate, and tetrasodium iminodisuccinate.
In the experimental process, the applicant finds that the added carboxymethyl chitosan can form a further micro-crosslinking phenomenon with antibacterial peptide, complexing agent tetrasodium aminodisuccinate and electrolytic silver water, and the antibacterial peptide can be attached to the surface of the carboxymethyl chitosan to further coat the electrolytic silver water; in addition, the existence of the amino disuccinic acid tetrasodium can form further complexation with silver ions, so that a slow release effect can be formed; in addition, in the experimental process, the applicant finds that the mass concentration of the electrolytic silver water and the weight ratio of the electrolytic silver water to the antibacterial peptide have larger influence on the antibacterial effect, and the selected electrolytic silver water with the mass concentration of 5-30ppm can play a better antibacterial effect. When the mass concentration of the electrolytic silver water is too high, the electrolytic silver water can quickly permeate into the cell membranes of bacteria, the antibacterial effect takes effect immediately, but the action of protonated ammonia and the like in the system and negative charges of the bacteria is influenced, further interaction force of active groups in the system is caused, and the long-acting property of the antibacterial effect is reduced;
meanwhile, when the weight ratio of the electrolyzed silver water to the antibacterial peptide is 8:2.5, the antibacterial peptide can provide the condition of interaction with bacterial cell membranes, so that the long-acting effect is achieved, the occurrence of bacterial drug resistance is avoided, and meanwhile, the stability of the silver ion long-acting antibacterial composition is improved due to the synergistic effect of the antibacterial peptide and a stabilizer.
In some preferred embodiments, the raw materials for preparing the long-acting antibacterial composition containing silver ions comprise, by weight: 1-5 parts of electrolytic silver water, 0.1-2 parts of biomass polysaccharide, 0.1-1 part of antibacterial peptide, 0.1-2 parts of stabilizer, 0.1-1 part of complexing agent and 30-50 parts of water.
The second aspect of the present invention provides a method for preparing a long-acting antibacterial composition containing silver ions, comprising the steps of:
adding electrolytic silver water and antibacterial peptide into water, mixing, adding biomass polysaccharide and auxiliary agent, controlling reaction temperature at 30-45 ℃, mixing and reacting for 60-90 minutes, and cooling to 25 ℃ to obtain the antibacterial peptide.
In the process of preparing the long-acting antibacterial composition containing silver ions, the applicant finds that after the biomass polysaccharide and the auxiliary agent are added, the electrolyzed silver water and the antibacterial peptide can be ensured to form a complex structure, the slow-release effect of the electrolyzed silver water can be formed, and the antibacterial effect can be obviously prolonged compared with the effective period of a common silver ion antibacterial agent.
Has the advantages that: the silver ion long-acting antibacterial composition prepared by the invention has the following advantages:
1. the mass concentration of the silver ion long-acting antibacterial composition prepared by the invention in electrolytic silver water is 5-30ppm, so that the long-acting antibacterial effect can be ensured, the accumulation of silver ions in the environment or human body is avoided, and the trace efficient antibacterial effect is realized;
2. the silver ion long-acting antibacterial composition prepared by the application can prolong the antibacterial effect of a system and the action of the antibacterial composition and a bacterial cell membrane under the synergistic action of the chitosan derivative and the antibacterial peptide, the stability of the antibacterial composition is enhanced, and the occurrence of bacterial drug resistance is avoided.
Detailed Description
Examples
Example 1
A long-acting antibacterial composition containing silver ions comprises the following raw materials in parts by weight: 3 parts of electrolytic silver water, 1.2 parts of carboxymethyl chitosan, 0.9 part of antibacterial peptide, 1 part of stabilizer, 0.4 part of complexing agent and 35 parts of water.
The electrolytic silver water has the mass concentration of 15ppm and is purchased from Japan oasis corporation.
Carboxymethyl chitosan, cat # C832672, available from michelin reagent net.
The antibacterial peptide is selected from Human beta-defensin-3, CAS:221230-01-9, available from MedChemexpress LLC of the United states.
The stabilizing agent is citric acid.
The complexing agent is tetrasodium iminodisuccinate.
The preparation method of the silver ion long-acting antibacterial composition comprises the following steps:
adding electrolytic silver water and antibacterial peptide into water, mixing, adding biomass polysaccharide and auxiliary agent, controlling reaction temperature at 35 ℃, mixing and reacting for 75 minutes, and cooling to 25 ℃ to obtain the antibacterial peptide.
Example 2
A long-acting antibacterial composition containing silver ions comprises the following raw materials in parts by weight: 3 parts of electrolytic silver water, 1.2 parts of carboxymethyl chitosan, 0.3 part of antibacterial peptide, 1 part of stabilizer, 0.4 part of complexing agent and 35 parts of water.
The electrolytic silver water has the mass concentration of 15ppm and is purchased from Japan oasis corporation.
Carboxymethyl chitosan, cat # C832672, available from michelin reagent net.
The antibacterial peptide is selected from Human beta-defensin-3, CAS:221230-01-9, available from MedChemexpress LLC of the United states.
The stabilizing agent is citric acid.
The complexing agent is tetrasodium iminodisuccinate.
The preparation method of the long-acting antibacterial composition containing silver ions comprises the following steps:
adding electrolytic silver water and antibacterial peptide into water, mixing, adding biomass polysaccharide and auxiliary agent, controlling reaction temperature at 35 ℃, mixing and reacting for 75 minutes, and cooling to 25 ℃ to obtain the antibacterial peptide.
Example 3
A long-acting antibacterial composition containing silver ions comprises the following raw materials in parts by weight: 3 parts of electrolytic silver water, 1.2 parts of chitosan, 0.5 part of antibacterial peptide, 1 part of stabilizer, 0.4 part of complexing agent and 35 parts of water.
The electrolytic silver water has a mass concentration of 15ppm and is purchased from Japan oasis.
Chitosan, purchased from zibozerun biotechnology limited;
the antibacterial peptide is selected from Human beta-defensin-3, CAS:221230-01-9, available from MedChemexpress LLC, USA.
The stabilizing agent is citric acid.
The complexing agent is tetrasodium iminodisuccinate.
The preparation method of the silver ion long-acting antibacterial composition comprises the following steps:
adding electrolytic silver water and antibacterial peptide into water, mixing, adding biomass polysaccharide and auxiliary agent, controlling reaction temperature at 35 ℃, mixing and reacting for 75 minutes, and cooling to 25 ℃ to obtain the antibacterial peptide.
Example 4
A long-acting antibacterial composition containing silver ions comprises the following raw materials in parts by weight: 3 parts of electrolytic silver water, 1.2 parts of carboxymethyl chitosan, 0.5 part of antibacterial peptide, 0.4 part of complexing agent and 35 parts of water.
The electrolytic silver water has the mass concentration of 15ppm and is purchased from Japan oasis corporation.
Carboxymethyl chitosan, cat # C832672, available from michelin reagent net.
The antibacterial peptide is selected from Human beta-defensin-3, CAS:221230-01-9, available from MedChemexpress LLC of the United states.
The complexing agent is tetrasodium iminodisuccinate.
The preparation method of the silver ion long-acting antibacterial composition comprises the following steps:
adding electrolytic silver water and antibacterial peptide into water, mixing, adding biomass polysaccharide and an auxiliary agent, controlling the reaction temperature to be 35 ℃, mixing and reacting for 75 minutes, and cooling to 25 ℃ to obtain the antibacterial peptide.
Example 5
A long-acting antibacterial composition containing silver ions comprises the following raw materials in parts by weight: 3 parts of electrolytic silver water, 1.2 parts of carboxymethyl chitosan, 0.5 part of antibacterial peptide, 1 part of stabilizer, 0.4 part of complexing agent and 35 parts of water.
The electrolytic silver water has the mass concentration of 15ppm and is purchased from Japan oasis corporation.
Carboxymethyl chitosan, cat # C832672, available from michelin reagent net.
The antibacterial peptide is selected from Human beta-defensin-3, CAS:221230-01-9, available from MedChemexpress LLC of the United states.
The stabilizing agent is citric acid.
The complexing agent is EDTA-2Na.
The preparation method of the silver ion long-acting antibacterial composition comprises the following steps:
adding electrolytic silver water and antibacterial peptide into water, mixing, adding biomass polysaccharide and an auxiliary agent, controlling the reaction temperature to be 35 ℃, mixing and reacting for 75 minutes, and cooling to 25 ℃ to obtain the antibacterial peptide.
And (3) performance testing:
1. and (3) testing antibacterial performance: the silver ion long-acting antibacterial composition prepared in example 1 of the present application and a commercially available (Nanjing Tianshi blue Shield Biotechnology Co., ltd., product No. 628) silver ion antibacterial agent were used for antibacterial performance test, the test strains were Klebsiella pneumoniae, candida albicans, and Pseudomonas aeruginosa, and the test was divided into two procedures:
1) The first test: the test was performed directly with example 1 and a commercially available antimicrobial and the results were recorded;
2) And (3) testing for the second time: placing the antibacterial composition prepared by the application and a commercially available antibacterial agent in the same environment, testing by using the same method after 90 days, and recording the experimental result; the results of the antibacterial property test are summarized in table 1.
Table 1:
Figure BDA0003268113960000051
Figure BDA0003268113960000061
2. and (3) stability testing: the antibacterial compositions prepared in examples 1 to 5 were used for stability testing, and were respectively placed at 20 c and 40 c for 90 days, and the changes were observed, and the results were counted in the following table 2.
Table 2:
Figure BDA0003268113960000062

Claims (10)

1. the silver ion-containing antibacterial composition is characterized by being prepared from the following raw materials: electrolyzed silver water, biomass polysaccharide, antibacterial peptide, auxiliary agent and water;
the biomass polysaccharide is at least one selected from sodium alginate, chitosan, carrageenan, gelatin, carboxymethyl cellulose and derivatives thereof.
2. The silver ion-containing antibacterial composition of claim 1, wherein the electrolytic silver water has a mass concentration of 5 to 30ppm.
3. The silver-ion containing antimicrobial composition of claim 1, wherein said biomass polysaccharide is chitosan.
4. The silver ion-containing antimicrobial composition of claim 3, wherein said chitosan is a modified chitosan.
5. The silver ion-containing antimicrobial composition of claim 1, wherein the weight ratio of the electrolyzed silver water to the antimicrobial peptide is 8: (1-5).
6. The silver ion-containing antimicrobial composition of claim 7, wherein the weight ratio of the electrolyzed silver water to the antimicrobial peptide is 8:2.5.
7. the silver ion-containing antibacterial composition of claim 1, wherein the auxiliary agent is at least one selected from the group consisting of a dispersant, a stabilizer, a complexing agent, and a pH adjuster.
8. The silver-ion-containing antimicrobial composition of claim 6, wherein the stabilizer is at least one selected from the group consisting of glucose and citric acid.
9. The silver-ion-containing antimicrobial composition according to claim 7, wherein the complexing agent is at least one selected from the group consisting of tetrasodium glutamate diacetate, trisodium methylglycinediacetate, and tetrasodium iminodisuccinate.
10. A method of preparing an antimicrobial composition containing silver ions according to any one of claims 1 to 9, comprising the steps of:
adding electrolytic silver water and antibacterial peptide into water, mixing, adding biomass polysaccharide and auxiliary agent, controlling reaction temperature at 30-45 deg.C, mixing and reacting for 60-90 min, and cooling to 25 deg.C to obtain the final product.
CN202111092640.6A 2021-09-17 2021-09-17 Silver ion-containing antibacterial composition and preparation method thereof Withdrawn CN115363021A (en)

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Application publication date: 20221122