CN115362857A - Method for preventing and treating rice bacterial wilt - Google Patents

Method for preventing and treating rice bacterial wilt Download PDF

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CN115362857A
CN115362857A CN202211158828.0A CN202211158828A CN115362857A CN 115362857 A CN115362857 A CN 115362857A CN 202211158828 A CN202211158828 A CN 202211158828A CN 115362857 A CN115362857 A CN 115362857A
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rice
spraying
seedbed
bacterial wilt
medicament
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李真峰
梁文涛
冯辉
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Zhejiang Shiyuan Jinniu Chemical Co ltd
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Zhejiang Shiyuan Jinniu Chemical Co ltd
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    • AHUMAN NECESSITIES
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    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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    • A01N43/80Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with nitrogen atoms and oxygen or sulfur atoms as ring hetero atoms five-membered rings with one nitrogen atom and either one oxygen atom or one sulfur atom in positions 1,2
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
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    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
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    • AHUMAN NECESSITIES
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    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
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Abstract

The invention discloses a method for preventing and treating rice bacterial wilt, which comprises the steps of carrying out medicament spraying prevention or spraying treatment firstly when rice seedlings stand to reach the stage of one leaf and one heart, then carrying out clear water spraying seedbed seedling washing, and finally carrying out crop spraying by using alginic acid aqueous solution, wherein the medicament comprises a medicament combination A or a medicament combination B, the medicament combination A comprises 50 ml of Kejia, hymexazol and Wajiafu respectively, mixing the medicament combination A and the medicament combination A with 15-30L of water for dilution for later use, the spraying prevention operation surface of the medicament combination A with the dosage is a 120 square meter rice seedbed, and the spraying treatment operation surface is a 60 square meter rice seedbed; the medicament combination B comprises 25 ml of fushuaide, 50 ml of hymexazol and 50 ml of Woljiafu respectively, and the mixture is mixed and diluted by 15 to 30 liters of water for standby application, and the application method is the same as that of the medicament combination A. The invention leads the rice seedlings to grow healthier while avoiding the harm of damping off by a mixed application technology of killing high and low grade fungi and alginic acid with the functions of relieving pesticide damage and promoting the growth of the rice seedlings.

Description

Method for preventing and treating rice bacterial wilt
Technical Field
The invention relates to the technical field of rice seedling blight prevention and control, and particularly relates to a rice seedling blight prevention and control method applied to rice seedlings.
Background
The prior art of the rice damping-off can only solve the harm caused by one kind of bacteria, and actually, the damping-off is harmed by at least three kinds of bacteria in a mixed way. The pathogenic bacteria of the rice rhizoctonia solani can be more than 7, the most main pathogenic bacteria are three, namely, pythium, fusarium and rhizoctonia solani, wherein the pythium is low-grade fungi, and the rest of the pythium belongs to high-grade fungi. Therefore, the agent for preventing and controlling the rice damping-off must be effective to high and low fungi, and simultaneously, the chemical injury cannot occur. In the existing control technology of rice damping-off, a plurality of people try to control by using a medicament, when rice seedlings are damaged by only one kind of bacteria, the control effect of the existing technology is good, but most of the existing schemes are single-medicament control, the effect is unstable, the bacteria and the medicament can not be matched, and the effect is not good. The bactericidal drugs are various in types, mild in some types and general in effect, while the bactericidal drugs are strong in nature and good in bactericidal effect in some types, the pathogenic bacteria of the rice damping-off are prevented and treated, and the pesticide damage to the rice crops is easily caused.
Disclosure of Invention
In order to solve the defects of the technology, the invention provides a method for preventing and controlling the rice bacterial wilt, which adopts the mixed application technology of killing high and low fungi and alginic acid with the functions of relieving pesticide damage and promoting the growth of rice seedlings, so that the rice seedlings can grow healthier while avoiding the damage of the bacterial wilt.
The technical scheme adopted by the invention for realizing the technical effects is as follows:
a method for preventing and treating rice bacterial wilt is applied to preventing and treating the bacterial wilt of rice seedlings, and comprises the following steps of spraying a medicament for preventing or treating in a stage of setting up a needle of each rice seedling to a 'one-leaf one-heart' stage, and then spraying clear water to a seedbed for seedling washing, wherein the medicament comprises a medicament combination A or a medicament combination B:
the medicament combination A and the dosage thereof are as follows:
kejia 50 ml
Hymexazol 50 ml
Wojiafu 50 ml
Mixing, adding 15-30L of water for dilution for later use;
the spraying prevention operation surface of the pesticide combination A with the dosage is a rice seedbed with 120 square meters, and the spraying treatment operation surface is a rice seedbed with 60 square meters;
the medicament combination B and the dosage thereof are as follows:
fortune is 25 ml
Hymexazol 50 ml
Wojiafu 50 ml
Mixing, adding 15-30L of water for dilution for later use;
the spraying prevention operation surface of the pesticide combination B is a rice seedbed with the square meter of 120, and the spraying treatment operation surface is a rice seedbed with the square meter of 60.
Preferably, in the method for controlling rice bacterial wilt disease, the spraying prevention operation is performed in the early stage of needle setting of the rice seedlings, and the spraying treatment operation is performed in the middle or late stage of growing the rice seedlings in a way of growing one leaf and one heart.
Preferably, in the method for controlling rice bacterial wilt, the operation of spraying the clear water to the seedbed to wash the seedlings is within 2 to 3 hours after the operation of spraying prevention or spraying treatment is completed.
Preferably, in the method for controlling the rice bacterial wilt, the water consumption for washing the seedlings by spraying the clean water on the seedbed is 30-40 liters per 100 square meters of the seedbed of the rice.
Preferably, in the method for controlling rice bacterial wilt, the alginic acid water-fertilizer solution is also applied after the seedbed is washed by spraying clear water.
The beneficial effects of the invention are as follows: the invention adopts the mixed application technology of killing high and low grade fungi and alginic acid with the functions of relieving pesticide damage and promoting the growth of rice seedlings, so that the rice seedlings can grow healthier while avoiding the damage of damping off.
Detailed Description
For a further understanding of the invention, reference will now be made to the following examples which are set forth in part by way of illustration:
example 1:
a method for preventing and treating rice bacterial wilt is applied to preventing and treating the bacterial wilt of rice seedlings, and comprises the steps of respectively carrying out medicament spraying prevention or spraying treatment when the rice seedlings stand to reach the stage of one leaf and one heart, and then carrying out clear water spraying on a seedbed to wash the seedlings, wherein the medicaments comprise a medicament combination A;
the medicament combination A and the dosage thereof are as follows:
kejia 50 ml
Hymexazol 50 ml
Wojiafu 50 ml
Mixing, adding 15-30L of water, and diluting for later use;
the spraying prevention operation surface of the pesticide combination A is a rice seedbed with 120 square meters, and the spraying treatment operation surface is a rice seedbed with 60 square meters.
Further, in example 1, the spray prevention work was performed at the initial stage of the needle raising of the young rice seedlings, and the spray treatment work was performed at the middle or late stage of the growth of the young rice seedlings in "one leaf and one heart". The clean water spraying seedbed seedling washing operation is carried out within 2-3 hours after the spraying prevention or spraying treatment operation is finished. Wherein the water consumption for spraying clear water on the seedbed to wash the seedlings is 30-40 liters per 100 square meters of the rice seedbed.
Example 2:
a method for preventing and treating rice bacterial wilt is applied to preventing and treating the bacterial wilt of rice seedlings, and comprises the steps of respectively carrying out medicament spraying prevention or spraying treatment when the rice seedlings stand to reach the stage of one leaf and one heart, and then carrying out clear water spraying on a seedbed to wash the seedlings, wherein the medicaments comprise a medicament combination B;
the medicament combination B and the dosage thereof are as follows:
fortune is 25 ml
Hymexazol 50 ml
Wojiafu 50 ml
Mixing, adding 15-30L of water, and diluting for later use;
the spraying prevention operation surface of the pesticide combination B is a rice seedbed with the square meter of 120, and the spraying treatment operation surface is a rice seedbed with the square meter of 60.
Further, in example 2, the spray prevention work was performed at the initial stage of the needle raising of the young rice seedlings, and the spray treatment work was performed at the middle or late stage of the growth of the young rice seedlings in "one leaf and one heart". The clean water is sprayed on the seedbed to wash the seedlings within 2 to 3 hours after the spraying prevention or spraying treatment operation is finished. Wherein the water consumption for spraying clear water to the seedbed to wash the seedlings is 30-40 liters per 100 square meters of the rice seedbed.
To verify the preventive effect of combination a, the following analysis was carried out in combination with the specific test examples:
1. purpose of the experiment
The purpose of the test is as follows: the effect of the drug combination A on preventing bacterial wilt of rice at the early stage of vertical acupuncture is verified.
2. General description of the test site
The names of the farmers: zheng Zhongyue
Natural paddy field, and good irrigation condition.
3. Location and time of experiment
Test site: yunhang district ren and Zhengyongtai village
Test time: no. 4/16
4. Test arrangement and design
The rice seedbed is divided into units at intervals of 120 square meters, the units are blocked by partition plates, 8 test blocks which are not adjacent to each other are arranged, wherein 1 test block is subjected to test treatment, and the rest 7 test blocks are subjected to comparison treatment. In order to ensure the accuracy of the test and the consistency of the rice planting density of each test block, the test can be completed through a rice planter, and the manual error interference is avoided. Specifically, the planting density was set to 10cm inter-plant distance.
Test example 1: the procedure is carried out as described in example 1.
Comparative example 1: the procedure was as in example 1 except that only 50 ml of hymexazol was applied.
Comparative example 2: the procedure was as in example 1 except that 50 ml of the solution was used.
Comparative example 3: the procedure was as in example 1 except that only 50 ml of hymexazol and Wo Gufu ml were applied.
Comparative example 4: the procedure was as in example 1 except that only 50 ml of Kejia and Wo Gufu ml were applied.
Comparative example 5: the procedure was as in example 1 except that only 50 ml of Kejia and 50 ml of hymexazol were applied.
Comparative example 6: the method is the same as the method 1 except that only 50 ml of Kejia and 50 ml of hymexazol are applied, and the seedlings are not sprayed and washed by clear water.
Comparative example 7: blank control.
5. Test survey and result analysis
The investigation method comprises the following steps: five-point sampling method. After the above-mentioned test, visual observation was carried out one month after the rice seedling was inserted, and it was found that the disease rate of the rice suffering from the bacterial wilt disease was high in the field of comparative example 7. The total count of the rice bacterial wilt disease strains is determined by using a 100x100 cm iron frame, taking 5 frames of data of the test example and the comparative example respectively, and the data are respectively as follows:
TABLE 1
Figure BDA0003858515730000061
6. Test results
The use of example 1 for preventing rice bacterial wilt in the early stage of the needle setting can significantly and effectively reduce the incidence of rice bacterial wilt. In test example 1 and comparative examples 1 to 5, no dead diseased plants were seen (dead diseased plants appeared later as the growth period continued), and in test example 1, only 3 diseased plants with disease grade 1 were seen, and in comparative examples 1 to 5, the disease grade of the diseased plants was also mostly grade 1 or 2, and the number of the diseased plants of grade 1 was extremely large, and distributed in the interval of 90 to 95%, and no diseased plants of grade 3 and 4 were seen, in comparative example 6, diseased plants of grade 3 and 4 began to appear, and the number was 18% of the total number of diseased plants, while in comparative example 7, nearly half of the number of diseased plants of grade 4 were present in addition to the disease grades of grade 1, grade 2, and grade 3.
7. Conclusion of the experiment
The experiment shows that the application of the embodiment 1 can effectively reduce the possibility of the rice bacterial wilt in the early stage of rice needle setting. Comparative examples 1 and 2 show that the application of 50 ml of hymexazol or 50 ml of hymexazol can not effectively reduce the incidence rate of the rice bacterial wilt disease greatly. The reason is that hymexazol only has good control effect on lower fungi, but has limited control effect on other higher fungi, and Kejia can not effectively prevent the occurrence of rice bacterial wilt when used alone. The comparative examples 3 and 4 show that when hymexazol is combined with fujiafu or kejiafu, the prevention effect of the rice bacterial wilt can be improved to a certain extent, and on the basis of the comparative examples 1 and 2, the prevention effect can be further enhanced, and through observation and analysis, the reason is that the hymexazol or kejiafu are respectively compounded with the fujiafu to provide the rice seedlings with alginic acid fertilizer, promote the growth of the rice seedlings and improve the disease resistance of the rice seedlings against the bacterial wilt. In combination with the comparative example 5, the control effect on the rice seedling bacterial wilt is better than that of the comparative examples 1, 2, 3 and 4 when the hymexazol and the Kejia are compounded for use, because the hymexazol and the Kejia have a synergistic effect and can further improve the disease resistance of the rice seedling to the bacterial wilt. In contrast, in comparative example 6, when hymexazol was used well in combination with comparative example 5, it was found that the number of plants having a bacterial wilt disease in rice was increased by a significant amount as compared with comparative example 3 and comparative example 4, particularly as compared with comparative example 5, and also as compared with comparative example 1 and comparative example 2. It was found from the observation in the combination test that the rice in comparative example 6 had some traces of the toxicity of the drug, thereby causing a decrease in the disease resistance against the fungi that cause the bacterial wilt of rice. Therefore, when the hymexazol is used in combination with the Kejia, if the seedling washing operation is not carried out, the hymexazol and the Kejia compound medicine remained on the rice seedlings have a certain toxic action on rice plants, so that the disease resistance of the rice seedlings to the bacterial wilt cannot be increased, the disease resistance of the rice seedlings to fungi causing the bacterial wilt of rice can be reduced, and the probability of preventing the rice seedlings from the bacterial wilt of rice is increased. It can be known from the combination of the test example 1 and the comparative example 5 that the Wolfu added in the test example 1 can produce a certain detoxification effect, can reduce the toxicity of the drug to the rice without affecting the killing effect on the fungi causing the rice bacterial wilt, and has a synergistic effect when treating the rice bacterial wilt. According to the combination of the test example 1, the comparative example 5 and the comparative example 6, under the cooperation of seedling washing, the combination of Wolffia can generate a certain detoxification effect, so that the toxicity of the drug to the rice can be reduced, and the killing effect on fungi causing the rice bacterial wilt cannot be influenced.
To verify the therapeutic effect of combination a, the following analysis was performed in conjunction with specific test examples:
1. purpose of the experiment
The purpose of the test is as follows: the effect of the drug combination A on the bacterial wilt of rice in the stage of 'one leaf and one heart' is verified.
2. General description of the test site
The name of the farmer: zheng Zhongyue
Natural paddy field, and good irrigation condition.
3. Location and time of experiment
Test site: yunhang district ren and Zhengyongtai village
Test time: no. 5/8
4. Test arrangement and design
A blank control field which is not subjected to rice bacterial wilt prevention and control is taken as a test object and is divided into 8 test blocks which are not adjacent to each other, and the test blocks are separated from the adjacent non-test blocks by partition plates. The area size of each test block was set to 60 square meters. In order to ensure the accuracy of the test, the rice planting density of the blank control field is consistent, the test can be completed through a rice transplanter, and the manual error interference is avoided. Specifically, the planting density was set to 10cm inter-plant distance.
Test example 1: the procedure is as in example 1.
Comparative example 1: the procedure was as in example 1 except that only 50 ml of hymexazol was applied.
Comparative example 2: the procedure was as in example 1 except that 50 ml of the solution was used.
Comparative example 3: the procedure was as in example 1 except that only 50 ml of hymexazol and Wo Gufu ml were applied.
Comparative example 4: the procedure was as in example 1 except that only 50 ml of Kejia and Wo Gufu ml were applied.
Comparative example 5: the procedure was as in example 1 except that only 50 ml of Kejia and 50 ml of hymexazol were applied.
Comparative example 6: the procedure was as in example 1 except that only 50 ml of Kejia and 50 ml of hymexazol were applied and no spraying of clean water was applied to wash the seedlings.
Comparative example 7: blank control.
5. Test investigation and result analysis
The investigation method comprises the following steps: five sampling method. After the above-described test, visual observation was carried out after one month when the young rice seedlings entered the "one-leaf one-heart" stage, and it was found that the retention rate of diseased plants was the highest in the field of comparative example 7. The total count of the rice bacterial wilt disease strains is calculated by using a 100x100 cm iron frame, taking 5 frames of data in the test example and the comparative example (each frame ensures that the number of the disease strains suffering from the rice bacterial wilt disease is consistent), and the data are respectively as follows:
TABLE 1
Figure BDA0003858515730000091
Figure BDA0003858515730000101
6. Test results
In comparative example 6, 49 diseased plants still alive and the remaining diseased plants died, in comparative example 7, 62 diseased plants still alive and the remaining diseased plants died, in test example 1 and comparative examples 1 to 5, no dead diseased plants were found, and in test example 1, only 4 diseased plants having a disease grade of 1 were found, and the diseased plants in comparative examples 1 to 5 were also mostly rated at 1 or 2, and no diseased plants of 3 and 4. In comparative example 6, the disease strains of grade 1, grade 2, grade 3 and grade 4 were distributed in approximately similar numbers, and there were 4 disease strains that were nearly dead.
7. Conclusion of the experiment
The experiment shows that the diseased rice plants can be effectively treated by using the example 1 during the period of one leaf and one heart of the rice, and the diseased rice plants with the disease grade at the first grade and the second grade can be effectively treated. Comparative examples 1 and 2 show that the disease of the rice bacterial wilt disease cannot be effectively and greatly treated by only applying 50 ml of hymexazol or only 50 ml of Kejia 50 ml. The reason is that hymexazol only has good control effect on lower fungi, but has limited control effect on other higher fungi. Kejia can not effectively treat the rice bacterial wilt disease when used alone. In contrast, it can be found from comparative example 3 and comparative example 4 that when hymexazol is used in combination with wolfurf or kohlrabi is used in combination with wolfurf, the effect of treating rice bacterial wilt can be improved to some extent compared with comparative example 1 and comparative example 2, because hymexazol or kohlrabi is used in combination with wolfurf respectively, alginic acid fertilizer can be provided for rice seedlings to promote the growth of the rice seedlings and improve the disease resistance of the rice seedlings against the bacterial wilt, but the effect of treating rice bacterial wilt is not better than that of hymexazol in combination with kohlrabi in comparative example 5. In comparative example 5, the control effect of the hymexazol compound for controlling the bacterial wilt of rice is better than that of comparative example 1, comparative example 2, comparative example 3 and comparative example 4, because the hymexazol and the hymexazol compound for controlling the bacterial wilt of rice have a synergistic effect, and the disease resistance of the rice against the bacterial wilt can be further improved. In the comparative example 6, when the hymexazol combined plant is used well, the total number of the diseased plants is still maintained to be higher than that in the comparative example 3 and the comparative example 4 after spraying treatment, and is obviously increased compared with that in the comparative example 1 and the comparative example 2, and in the combined test, the rice in the comparative example 6 has a certain degree of drug poisoning traces, so that the disease resistance of the fungi causing the rice bacterial wilt is reduced. Therefore, when the hymexazol is preferably used in combination with the rice, if the rice is not subjected to seedling washing operation, the residual drug on the rice has a certain toxic action on rice plants, but also can reduce the disease resistance of the rice to fungi causing the rice bacterial wilt, and increases the risk that the rice with the disease (rice bacterial wilt) grade of 1 or 2 is continuously deteriorated to the grade 3 or 4 to aggravate the disease. According to the combination of the test example 1 and the comparative example 6, under the coordination of seedling washing, the combination of Woljiafu can generate a certain detoxification effect, so that the toxicity of the drug to rice can be reduced, and the killing effect on fungi causing rice bacterial wilt cannot be influenced.
The test of the prevention effect and the treatment effect of the drug combination B on the rice bacterial wilt disease is the same as the test of the prevention effect and the treatment effect of the drug combination A on the rice bacterial wilt disease, the difference is only that the drug combination B is different from the drug combination A, the data recorded by the test shows that the drug combination B also has better prevention and treatment effects on the rice bacterial wilt disease, and the action principle is the same as the verification test of the prevention effect and the treatment effect of the drug combination A on the rice bacterial wilt disease.
The invention adopts the mixed application technology of killing high and low grade fungi and alginic acid with the functions of relieving pesticide damage and promoting the growth of rice seedlings, so that the rice seedlings can grow healthier while avoiding the damage of damping off.
The foregoing shows and describes the general principles, essential features, and advantages of the invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are merely illustrative of the principles of the invention, but that various changes and modifications may be made without departing from the spirit and scope of the invention, which is defined by the appended claims and their equivalents.

Claims (4)

1. The method for preventing and treating the rice bacterial wilt is characterized by being applied to the prevention and treatment of the rice bacterial wilt, firstly carrying out medicament spraying prevention or spraying treatment when the rice seedlings stand to the stage of one leaf and one heart, and then carrying out clear water spraying on a seedbed to wash the seedlings, wherein the medicaments comprise a medicament combination A or a medicament combination B:
the medicament combination A and the dosage thereof are as follows:
kejia 50 ml
Hymexazol 50 ml
Wojiafu 50 ml
Mixing, adding 15-30L of water, and diluting for later use;
the spraying prevention operation surface of the pesticide combination A with the dosage is a rice seedbed with 120 square meters, and the spraying treatment operation surface is a rice seedbed with 60 square meters;
the medicament combination B and the dosage thereof are as follows:
fortune is 25 ml
Hymexazol 50 ml
Wojiafu 50 ml
Mixing, adding 15-30L of water for dilution for later use;
the spraying prevention operation surface of the pesticide combination B is a rice seedbed with the square meter of 120, and the spraying treatment operation surface is a rice seedbed with the square meter of 60.
2. The method for controlling rice bacterial wilt according to claim 1, wherein the spray prevention operation is performed at the early stage of needle setting of a rice seedling, and the spray treatment operation is performed at the middle or late stage of growth of the rice seedling in the form of "one leaf and one heart".
3. The method for controlling rice bacterial wilt according to claim 1, wherein the operation of spraying clear water to a seedbed for seedling washing is performed within 2 to 3 hours after the operation of spraying prevention or spraying treatment is completed.
4. The method for controlling rice bacterial wilt according to claim 3, wherein the water consumption for spraying the clear water to the seedbed to wash the seedlings is 30-40L per 100 square meters of the seedbed of the rice.
CN202211158828.0A 2022-09-22 2022-09-22 Method for preventing and treating rice bacterial wilt Pending CN115362857A (en)

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