CN115350217A - Application of fructus Rosae Laevigatae or fructus Rosae Laevigatae water extract in preparing medicine for promoting skin wound repair - Google Patents
Application of fructus Rosae Laevigatae or fructus Rosae Laevigatae water extract in preparing medicine for promoting skin wound repair Download PDFInfo
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
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- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/738—Rosa (rose)
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
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- A61P17/02—Drugs for dermatological disorders for treating wounds, ulcers, burns, scars, keloids, or the like
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- A61K2236/30—Extraction of the material
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- A61K2236/331—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using water, e.g. cold water, infusion, tea, steam distillation, decoction
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- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention relates to application of cherokee rose fruit or aqueous extract of cherokee rose fruit as a unique active component in preparing a medicament for promoting skin wound repair, wherein the cherokee rose fruit is powder of dry fruits, and the preparation method of the aqueous extract of cherokee rose fruit comprises the following steps: taking dried cherokee rose fruit, crushing, adding water, uniformly stirring, performing immersion treatment, performing ultrasonic treatment, filtering and centrifuging after treatment, taking supernate, concentrating the supernate, and cooling the obtained concentrated solution to obtain the cherokee rose fruit water extract. The invention breaks through the limitation that only antibiotics and local disinfection are used in the traditional clinical wound care and the healing is realized by completely depending on the self-healing capability of a patient and the long-time tissue remodeling effect, applies the cherokee rose fruit or the aqueous extract of the cherokee rose fruit to the field of skin wound surface repair, and has obvious repair effect.
Description
Technical Field
The invention relates to the technical field of skin wound repair, in particular to application of cherokee rose fruit or cherokee rose fruit aqueous extract in preparing a medicament for promoting skin wound repair.
Background
Skin wound repair is a hot problem in clinical medical research, and the quality of the repair effect directly affects the function and the beauty of the body, and even causes health problems such as pain, infection and the like. The skin wound repair is a healing process after trauma, relates to complex coordination effects of various growth factors, cells and the like, mainly comprises four stages of hemostasis and blood coagulation, inflammation, proliferation and tissue remodeling, and medicaments for promoting the skin wound repair sold on the market at present are mainly designed aiming at the four stages, such as hemostatic medicaments, anti-inflammatory medicaments, collagen synthesis promoting medicaments and the like. According to the source classification, the traditional Chinese medicine, the molecular medicine, the marine medicine and the like are mainly used.
The external traditional Chinese medicine has unique features on repairing skin wounds, and the Chinese traditional medicine theory brings the mechanism of promoting skin wound repair by the external traditional Chinese medicine into consideration: the modern medical research proves that the externally applied traditional Chinese medicine can promote the repair of skin wounds by various modes of inhibiting bacteria, sterilizing, promoting the angiogenesis of the wounds, secreting growth factors, synthesizing collagen, regulating the pH of the wounds and the like. Although molecular drugs also play an important role in skin wound repair, due to the high price, the search for high-efficiency and low-price drugs is always a hot spot of medical research, and traditional Chinese medicines have the characteristics of high efficiency, low toxic and side effects and the like, and become an important screening library for the research and development of related innovative drugs in the world.
Disclosure of Invention
The invention aims to overcome the defects of the existing skin wound repair technology and provides a new application of cherokee rose fruit or a cherokee rose fruit aqueous extract, namely the cherokee rose fruit aqueous extract is used for preparing a medicament for promoting the repair of skin wounds.
The specific scheme is as follows:
the application of the fructus rosae laevigatae or the fructus rosae laevigatae aqueous extract as the only active component in preparing the medicine for promoting the skin wound repair.
Further, the cherokee rose fruit is powder of dried fruit.
Further, the preparation method of the aqueous cherokee rose fruit extract comprises the following steps: taking dried cherokee rose fruit, adding water after crushing, uniformly stirring for dipping treatment, then carrying out ultrasonic treatment, filtering and centrifuging after the treatment, taking supernatant, concentrating the supernatant, and cooling the obtained concentrated solution to obtain the cherokee rose fruit aqueous extract.
Further, the preparation method of the aqueous extract of cherokee rose-hip comprises the following steps: taking dried cherokee rose fruit, crushing, adding water according to the material-liquid ratio of 1-1.
Further, carrying out vacuum freeze drying on the concentrated solution at the temperature of between 0 and minus 20 ℃ for 24 to 48 hours to obtain the aqueous extract of the cherokee rose fruit.
The invention also provides a medicament for promoting skin wound repair, which takes the cherokee rose fruit or aqueous extract of the cherokee rose fruit as the only active component.
Further, the cherokee rose fruit is powder of dried fruit.
Further, the preparation method of the aqueous cherokee rose fruit extract comprises the following steps: taking dried cherokee rose fruit, crushing, adding water, uniformly stirring, carrying out immersion treatment, then carrying out ultrasonic treatment, filtering and centrifuging after the treatment, taking supernatant, concentrating the supernatant, and cooling the obtained concentrated solution to obtain the cherokee rose fruit aqueous extract;
preferably, dried cherokee rose fruit is taken, crushed, added with water according to the material-liquid ratio of 1-1.
Further, carrying out vacuum freeze drying on the concentrated solution at the temperature of between 0 and minus 20 ℃ for 24 to 48 hours to obtain the aqueous extract of the cherokee rose fruit.
Furthermore, the medicine for promoting the skin wound repair also comprises at least one pharmaceutically acceptable auxiliary material, and the medicine is prepared into any one of powder, solution, suspension, emulsion, foam, lotion, gel, liniment, serous fluid and spray.
Has the advantages that:
the cherokee rose fruit or cherokee rose fruit aqueous extract provided by the invention has obvious effect on repairing skin wound, and breaks through the limitation that only antibiotics and local disinfection are used in the traditional wound clinical care, and the wound is completely healed by the self-healing capability and long-time tissue remodeling action of a patient.
The medicine for promoting skin wound repair provided by the invention can relieve paralysis scar tissues and shorten wound healing time, has small toxic and side effects, and has important clinical value.
Drawings
In order to illustrate the technical solution of the present invention more clearly, the drawings will be briefly described below, and it is apparent that the drawings in the following description relate only to some embodiments of the present invention and are not intended to limit the present invention.
FIG. 1 is a graph showing the effect of aqueous extract of Cherokee rose on the repair of the HaCaT scratch of keratinocytes in accordance with one embodiment 2 of the present invention;
FIG. 2 is a graph showing the therapeutic effect of aqueous extract of Cherokee Rose on the repair of skin wounds of mice, according to an embodiment 3 of the present invention;
FIG. 3 is a graph showing the effect of aqueous extract of Cherokee Rose fruit on the expression levels of matrix metalloproteinase and collagen in fibroblast 3T3-L1 according to example 4 of the present invention.
Detailed Description
Definitions for some of the terms used in the present invention are given below, and other terms not described have definitions and meanings known in the art:
the variety source of the fructus rosae laevigatae is Rosa laevigata michx of rosaceous plants, and the dry mature fruits of the fructus rosae laevigatae are named as traditional Chinese medicines in the invention.
Preferred embodiments of the present invention will be described in more detail below. While the following describes preferred embodiments of the present invention, it should be understood that the present invention may be embodied in various forms and should not be limited by the embodiments set forth herein. Those skilled in the art will recognize that the specific techniques or conditions, not specified in the examples, are according to the techniques or conditions described in the literature of the art or according to the product specification. The reagents or instruments used are conventional products which are commercially available, and are not indicated by manufacturers. In the following examples, "%" means weight percent, unless otherwise specified.
The main reagents used below included:
HaCaT cells: cell bank of Chinese academy of sciences
Fibroblast 3T3-L1: cell bank of Chinese academy of sciences
A first antibody: wuhan Aibo Taike Biotech Co., ltd
Secondary antibody: jackson ImmunoResearch Laboratories, inc.
Example 1A method for preparing an aqueous extract of Cherokee Rose fruit
Taking a proper amount of dried cherokee rose fruit, crushing, and adding ddH according to the material-liquid ratio of 1 2 O (secondary distilled water), stirring, soaking at 4 deg.C for 12 hr, ultrasonic extracting at 45kHz for 30min, vacuum filtering, centrifuging at 4000rpm/min for 10min, collecting supernatant, rotary evaporating for concentration (55 deg.C), and vacuum freeze drying the concentrate for 48 hr to obtain fructus Rosae Laevigatae aqueous extract, and storing at-20 deg.C.
Example 2 Effect of aqueous extracts of Cherokee Rose on HaCaT scratch repair of keratinocytes
Using the aqueous extract of Cherokee Rose fruit prepared in example 1, ddH was added 2 And O, preparing the Cherokee rose fruit aqueous extract mother liquor with the concentration of 100 mg/mL.
The experiment was divided into blank and drug groups. Taking HaCaT cells in logarithmic growth phase according to the proportion of 5X 10 5 The amount of seed/well was seeded into 12-well plates and placed in CO 2 Incubator (37 ℃,5% CO) 2 ) Culturing until the cell confluence is close to 100%. Making cell scratch with 200 μ L micropipettor suction head vertical pore plate, washing with PBS for 1 time, adding culture medium into blank group, adding culture medium containing fructus Rosae Laevigatae water extract with final concentration of 100 μ g/mL into medicinal group, and adding into culture medium at 0h, 24h andthe photographic record is observed by a microscope for 48 h.
The closer the cell distance on both sides of the scratch, the smaller the scratch area, indicating a higher cell mobility. The experimental results are shown in figure 1, in which CON is a blank group, and compared with the blank group, the aqueous cherokee rose-hip extract shows significant cell migration promoting effect at 24h, 48h and 72 h.
The results show that the aqueous extract of cherokee rose-hip can accelerate the epithelialization process of the wound surface by promoting the migration of keratinocytes, thereby promoting the repair of the wound surface.
Example 3 Effect of aqueous extracts of Cherokee Rose on wound repair on the dorsal skin of mice
Using the aqueous extract of Cherokee Rose fruit prepared in example 1, ddH was added 2 And O, preparing the mother solution of the aqueous extract of the cherokee rose-hip with the concentration of 100mg/mL, and carrying out wound repair experiments.
After 10 mice were bred for 3 days, they were randomly divided into a blank group and a cherokee rose group. All mice were anesthetized by intraperitoneal injection (20% urethane), the hair on the back of the mice was removed, and a 1cm x 1cm full-thickness skin incision was made in the center of the dorsal spine of each mouse using a skin biopsy device and scissors. After the model building, the blank group is only treated by normal saline, mice of the cherokee rose group are treated by aqueous extract of cherokee rose fruit with low concentration (20 mg/kg/d) and high concentration (80 mg/kg/d), and 2 groups are treated continuously for 14d (1 time/d). The wound surface of each group was photographed on the day of surgery, 7 th and 14 th days after surgery, the wound surface healing rate was calculated, and the wound surface repair condition was evaluated.
The experimental result is shown in figure 2, in the figure, CON is a blank group, and compared with the blank group, the cherokee rose fruit aqueous extract can obviously promote the healing of the skin wound of a mouse and promote scabbing and falling off at 7d and 14 d.
Example 4 Effect of aqueous extracts of Cherokee Rose on the expression levels of matrix Metalloproteinase and collagen in fibroblasts 3T3-L1
Using the aqueous extract of Cherokee Rose fruit prepared in example 1, ddH was added 2 And O, preparing the Cherokee rose fruit aqueous extract mother liquor with the concentration of 100 mg/mL.
The experiment was divided into blank and drug groups. Taking fibroblast 3T3-L1 in logarithmic growth phase according to the ratio of 1 × 10 6 Of one/holeQuantitatively inoculated into 6-well plates and placed in CO 2 Incubator (37 ℃,5% 2 ) The culture was carried out overnight. The drug group was added with 100mg/mL of the mother solution of aqueous extract of Cherokee rose fruit to a final concentration of 10, 100 and 200. Mu.g/mL, respectively, and cultured for further 24 hours. The total protein extract is obtained after cell lysis and centrifugation and the protein concentration is measured. Protein electrophoresis was performed by SDS-PAGE, and the proteins were transferred to PVDF membrane at a constant pressure of 100V for 1 hour. The PVDF membrane was placed in a TBST blocking solution containing 5% BSA for 1h at room temperature, followed by addition of primary antibody (1. TBST was washed three times and incubated for 1h at room temperature with the addition of a secondary antibody (1. After washing three times with TBST, ECL luminescence solution was added and imaged with a ChemiDoc XRS + system.
The collagen I and III (COL 1A1 and COL3A 1) are the main collagen components in skin tissues, and can promote the repair of skin wound surface when the content of the collagen I and III is increased, improve the healing quality of the wound surface, increase the elasticity and flexibility of new tissues and avoid the formation of scars. Matrix metalloproteinase 1 (MMP 1) can degrade a variety of collagens including types I, II, III, VII, VIII, and X; MMP2 can degrade basement membrane, collagen types V, VII, IX and X; MMP9 can degrade the main components of extracellular matrix and basement membrane, such as type IV collagen, type V collagen, adhesive protein and the like.
The experimental result is shown in figure 3, wherein CON is a blank group, and compared with the blank group, the cherokee rose fruit aqueous extract can obviously inhibit the expression of MMP1, MMP2 and MMP9 in the fiber cell 3T3-L1 and up-regulate the protein level of COL1A 1. The results show that the cherokee rose fruit aqueous extract can promote the expression of collagen by inhibiting MMPs, thereby accelerating the formation of extracellular matrix, promoting wound repair and improving the quality of wound repair.
The preferred embodiments of the present invention have been described in detail, however, the present invention is not limited to the specific details of the above embodiments, and various simple modifications may be made to the technical solution of the present invention within the technical idea of the present invention, and these simple modifications are all within the protection scope of the present invention.
It should be noted that the various features described in the above embodiments may be combined in any suitable manner without departing from the scope of the invention. The invention is not described in detail in order to avoid unnecessary repetition.
In addition, any combination of the various embodiments of the present invention can be made, and the same should be considered as the disclosure of the present invention as long as the idea of the present invention is not violated.
Claims (10)
1. The application of the fructus rosae laevigatae or the fructus rosae laevigatae aqueous extract as the only active component in preparing the medicine for promoting the skin wound repair.
2. Use according to claim 1, characterized in that: the fructus Rosae Laevigatae is dried fruit powder.
3. Use according to claim 1, characterized in that: the preparation method of the aqueous extract of cherokee rose fruit comprises the following steps: taking dried cherokee rose fruit, adding water after crushing, uniformly stirring for dipping treatment, then carrying out ultrasonic treatment, filtering and centrifuging after the treatment, taking supernatant, concentrating the supernatant, and cooling the obtained concentrated solution to obtain the cherokee rose fruit aqueous extract.
4. Use according to claim 3, characterized in that: the preparation method of the aqueous cherokee rose-hip extract comprises the following steps: taking dried cherokee rose fruit, crushing, adding water according to the material-liquid ratio of 1-1.
5. Use according to claim 3 or 4, characterized in that: and (3) carrying out vacuum freeze drying on the concentrated solution at the temperature of between 0 and minus 20 ℃ for 24 to 48 hours to obtain the aqueous extract of the cherokee rose fruit.
6. A medicine for promoting skin wound repair is characterized in that: the medicine takes cherokee rose fruit or aqueous extract of cherokee rose fruit as the only active component.
7. A medicament for promoting skin wound repair according to claim 6, wherein: the fructus Rosae Laevigatae is dried fruit powder.
8. A medicament for promoting skin wound repair according to claim 6, wherein: the preparation method of the aqueous cherokee rose-hip extract comprises the following steps: taking dried cherokee rose fruit, crushing, adding water, uniformly stirring, carrying out immersion treatment, then carrying out ultrasonic treatment, filtering and centrifuging after the treatment, taking supernatant, concentrating the supernatant, and cooling the obtained concentrated solution to obtain the cherokee rose fruit aqueous extract;
preferably, the cherokee rose fruit is dried, crushed, added with water according to the material-liquid ratio of 1-1.
9. The medicament for promoting skin wound repair according to claim 8, wherein: and (3) carrying out vacuum freeze drying on the concentrated solution at the temperature of between 0 and minus 20 ℃ for 24 to 48 hours to obtain the aqueous extract of the cherokee rose fruit.
10. A medicament for promoting skin wound repair according to any one of claims 6 to 9, wherein: the medicine for promoting the skin wound repair also comprises at least one pharmaceutically acceptable auxiliary material, and is prepared into any one of powder, solution, suspension, emulsion, foam, lotion, gel, liniment, serous fluid and spray.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211135281.2A CN115350217A (en) | 2022-09-19 | 2022-09-19 | Application of fructus Rosae Laevigatae or fructus Rosae Laevigatae water extract in preparing medicine for promoting skin wound repair |
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| CN202211135281.2A CN115350217A (en) | 2022-09-19 | 2022-09-19 | Application of fructus Rosae Laevigatae or fructus Rosae Laevigatae water extract in preparing medicine for promoting skin wound repair |
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| CN202211135281.2A Pending CN115350217A (en) | 2022-09-19 | 2022-09-19 | Application of fructus Rosae Laevigatae or fructus Rosae Laevigatae water extract in preparing medicine for promoting skin wound repair |
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Citations (4)
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| CN101829214A (en) * | 2010-05-21 | 2010-09-15 | 中国人民解放军第二军医大学 | Cherokee rose leaf extract and application thereof in preparing medicine capable of curing burn and scald |
| CN105213733A (en) * | 2015-10-29 | 2016-01-06 | 青岛海之源智能技术有限公司 | A kind of wound repair Chinese medicine soap and preparation method thereof |
| CN107126472A (en) * | 2017-05-04 | 2017-09-05 | 刘英雄 | A kind of burn-scald medicine masking liquid and preparation method thereof and application method |
| US20180296624A1 (en) * | 2017-04-17 | 2018-10-18 | Tci Co., Ltd | Preparations of rosa laevigata fruit extracts and uses thereof for regulation of cd36, apoa1, and srb1 |
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2022
- 2022-09-19 CN CN202211135281.2A patent/CN115350217A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101829214A (en) * | 2010-05-21 | 2010-09-15 | 中国人民解放军第二军医大学 | Cherokee rose leaf extract and application thereof in preparing medicine capable of curing burn and scald |
| CN105213733A (en) * | 2015-10-29 | 2016-01-06 | 青岛海之源智能技术有限公司 | A kind of wound repair Chinese medicine soap and preparation method thereof |
| US20180296624A1 (en) * | 2017-04-17 | 2018-10-18 | Tci Co., Ltd | Preparations of rosa laevigata fruit extracts and uses thereof for regulation of cd36, apoa1, and srb1 |
| CN107126472A (en) * | 2017-05-04 | 2017-09-05 | 刘英雄 | A kind of burn-scald medicine masking liquid and preparation method thereof and application method |
Non-Patent Citations (2)
| Title |
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| 梁薇: "中药免疫调节作用的研究进展", 现代医药卫生, no. 06, pages 674 - 676 * |
| 赵现敏;张红英;崔保安;李改英;邱妍;胡梅;王远阁;: "中草药免疫增强剂有效成分及其作用机制的研究概况", 中国畜牧兽医, vol. 2004, no. 11, pages 949 - 950 * |
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Application publication date: 20221118 |