CN115350170B

CN115350170B - Application of taurine in preparation of medicine for improving antiviral parotitis capability of Chinese softshell turtles

Info

Publication number: CN115350170B
Application number: CN202211040004.3A
Authority: CN
Inventors: 沈卫锋; 刘莉; 吕孙建; 郭琦; 翁宏飚; 韩明明; 楼宝
Original assignee: Zhejiang Academy of Agricultural Sciences
Current assignee: Zhejiang Academy of Agricultural Sciences
Priority date: 2022-08-29
Filing date: 2022-08-29
Publication date: 2023-08-29
Anticipated expiration: 2042-08-29
Also published as: CN115350170A

Abstract

The invention belongs to the technical field of animal immunity enhancer pharmacy, and particularly relates to application of taurine in preparation of a medicine for improving antiviral parotitis capability of Chinese soft-shelled turtles. Application of taurine in preparing medicine for treating and preventing viral parotitis is provided. The Chinese soft-shelled turtle is young soft-shelled turtle, and each young soft-shelled turtle weighing 20 g takes 120-600 mug taurine as injection dose. The invention proves that taurine has the capability of resisting the TSHSV virus of the Chinese soft shell turtle, provides a treatment method, can obviously improve the survival rate after virus infection, and provides a candidate substance for developing an antiviral immunity enhancer of the Chinese soft shell turtle.

Description

Application of taurine in preparation of medicine for improving antiviral parotitis capability of Chinese softshell turtles

Technical Field

The invention belongs to the technical field of animal immunity enhancer pharmacy, and particularly relates to application of taurine in preparation of a medicine for improving antiviral parotitis capability of Chinese soft-shelled turtles.

Background

Chinese soft-shelled turtleTrionyxsinensis) Belonging to the class of Leptospermum, the order of Tortoise, the family of Amydaceae, the genus of Amyda, commonly known as Amyda sinensis, is an important famous aquaculture variety in China. In recent years, chinese soft-shelled turtle breeding industry has been rapidly developed.

With the rise of industrial cultivation, the natural life habit of the Chinese soft-shelled turtles is challenged greatly by the reasons of high-density cultivation, germplasm degradation and the like, the disease resistance is reduced sharply, various diseases occur frequently, and great risks and severe risks are brought to the cultivation productionThe development of healthy cultivation of the Chinese soft-shelled turtles is seriously hindered. According to national Chinese soft-shelled turtle disease monitoring reports, 5 diseases such as red bottom plate disease, beancurd sheet disease, gastroenteritis disease, gill adenitis disease and ulcer syndrome are most common in the cultivation process, wherein the death rate of the gill adenitis disease is the highest, and the cultivation production is the most affected. In recent years, a plurality of Chinese soft-shelled turtle farms in Zhejiang province burst gill adenitis, serious bleeding symptoms appear in viscera and tissues of the Chinese soft-shelled turtles, the morbidity is urgent, the mortality rate is extremely high, and once the diseases occur, the Chinese soft-shelled turtles are almost completely covered within one week in the same culture pond. The applicant confirms and separates the pathogen of the gill adenitis to be a high-lethality virus, named Chinese soft-shelled turtle hemorrhagic syndrome virus @Trionyx Sinensis Hemorrhagic Syndrome Virμs，TSHSV) The whole gene sequence was determined.

The occurrence of the Chinese soft-shelled turtle virus disease is the result of the interaction among pathogenic pathogen, environmental condition and autoimmune capability, and no specific medicine aiming at the virus disease exists at present. Immunoprophylaxis is an important prevention and control measure for viral diseases, including specific immunity for vaccination and nonspecific immunity based on immunopotentiators. The safe and effective vaccine has long development period, specific pathogen, difficult aquatic animal immunization mode and other limitation in application and popularization. The immunopotentiator has the advantage of nonspecific immunity, can comprehensively improve the disease prevention capability of animal individuals, has stable general components, can be fed in a feed adding mode, and can achieve better disease prevention effect through standard use. However, the basic research related to the immunization of the Chinese soft-shelled turtles is still relatively lacking, and the development and the application of an immunopotentiator are hindered to a certain extent.

Taurine is a free beta-sulfur-containing amino acid in organisms, named after the first separation from the bile of bull, and is widely distributed in tissues and organs of mammals, birds, fish and aquatic invertebrates, accounting for 25% of the free amino acids in the liver. The existing researches show that taurine is an active substance for regulating normal physiological activities of organisms, and has wide biological functions of diminishing inflammation, easing pain, maintaining osmotic pressure balance of the organisms, regulating nerve conduction, participating in endocrine activities, regulating lipid digestion and absorption, increasing the systole capability, improving the immunity capability of the organisms, enhancing the oxidation resistance capability of cell membranes and the like. In aquatic animal production, taurine can be used as a phagostimulant to improve food intake and promote animal growth and development; can improve the stability of erythrocyte membrane and increase the content of hemoglobin in erythrocyte, thereby enhancing the anti-hypoxia capability of animals; the immunity of the organism can be enhanced by improving the activities of superoxide dismutase and lysozyme; can improve the oxidation resistance of cell membranes, and simultaneously can be combined with strong oxidants and free radicals in the body to prevent oxidation-reduction reaction, thereby avoiding oxidation injury; can also be used as neuromodulator to affect hypothalamic-pituitary hormone secretion levels. The research has proved that taurine can obviously improve the weight gain rate and specific growth rate of young Chinese softshell turtles, but no public report exists that taurine has the capability of resisting Chinese softshell turtle TSHSV virus.

Disclosure of Invention

In order to solve the technical problems, an object of the present invention is to provide an application of taurine or taurine derivatives used for replacing taurine in preparing anti-TSHSV virus drugs. Preferably, the dosage form of the medicine is one of injection, tablet, capsule and powder; alternatively, taurine or taurine derivatives used in place of taurine are added to the feed.

Furthermore, the invention also discloses application of taurine or taurine derivatives used for replacing taurine in preparation of medicines for treating and preventing the viral parotitis of the Chinese softshell turtles. Preferably, the Chinese soft-shelled turtle is young soft-shelled turtle. The young soft-shelled turtle with the weight of 20 g is injected with 120-600 mug taurine. Preferably, the dosage form of the medicine is one of injection, tablet, capsule and powder; alternatively, taurine or taurine derivative acid for replacing taurine is added to the feed.

The invention proves that taurine has the capability of resisting the TSHSV virus of the Chinese soft shell turtle, provides a treatment method, can obviously improve the survival rate after virus infection, and provides a candidate substance for developing an antiviral immunity enhancer of the Chinese soft shell turtle.

Drawings

Fig. 1: effect of taurine injections of different concentrations on survival after TSHSV infection of chinese softshell turtles. 600. The injected amount of μg/dose increased the survival rate by 43.4% 7 days after infection.

Fig. 2: pathological section results show that: taurine reduces liver bleeding and reduces occurrence of liver coagulation phenomena by protecting integrity of liver cell nuclei, reducing intercellular vacuoles, maintaining normal states of liver cells and other ways, so that liver tissues have important protection effects in the process of resisting TSHSV by Chinese soft shell turtles, the left graph is a TSHSV virus group, and the right graph is a 600 mug taurine+TSHSV group.

Detailed Description

1. The taurine solution is prepared and injected by the following method:

1) Weighing taurine 600 mg, dissolving in 1×PBS 100 ml, mixing, and standing at 4deg.C;

2) 100 μl of taurine was aspirated with a 1 ml syringe and injected into the abdominal cavity of Trionyx sinensis Wiegmann.

2. Virus (TSHSV) injection was performed as follows:

1) 100. Mu.l TSHSV virus solution (containing 100 copies) was aspirated with a 1 ml syringe;

2) 100 μl of the virus solution was injected into the hind leg muscle of Trionyx sinensis Wiegmann (20 g of young Trionyx sinensis Wiegmann).

3. The method for feeding and managing after the detoxification is as follows: after the toxicity is removed, the Chinese soft-shelled turtle is placed in a room at 28 ℃, 8 hours a day and 2 times a day at 5 pm, the survival condition is observed every day for 7 days.

4. The method for observing and judging the antiviral ability is as follows:

1) Counting death condition after 168 hours of toxin attack, and calculating death rate;

2) Dissecting livers of the live soft-shelled turtles and the dead soft-shelled turtles, taking 1 gram of liver tissue, adding 10 ml fixing solution, and carrying out slicing, HE dyeing and observation by biotechnology companies;

3) And comparing whether taurine is injected to protect the liver of young Chinese soft-shelled turtles.

The metabonomics is utilized to find that the content of taurine is obviously reduced after the TSHSV is infected, and if 600 mug/piece (20 g of young soft-shelled turtles) of taurine is supplemented in advance before the TSHSV is infected, the disease resistance of the Chinese soft-shelled turtles to the TSHSV can be improved. As shown in FIG. 1, the survival rate after 5 days of contamination is improved by 43.4%. As shown in fig. 2, the results of pathological sections of liver show that the addition of taurine can protect the integrity of liver cell nuclei, reduce intercellular vacuoles, maintain the normal state of liver cells, reduce liver bleeding, reduce the occurrence of coagulation phenomena, and demonstrate that liver tissues have important protective effects in the process of resisting TSHSV by Chinese soft-shelled turtles.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art. The generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. Preparation of taurine for improving Chinese soft-shelled turtle resistanceTSHSVUse of a virus capable drug.

2. The use according to claim 1, wherein the pharmaceutical dosage form is one of an injection, a tablet, a capsule, a powder.

3. Use according to claim 1, characterized in that taurine is added to the feed.

4. Use according to any one of claims 1-3, wherein the chinese soft-shelled turtle is a young soft-shelled turtle.

5. The use according to claim 4, wherein each young soft-shelled turtle weighing 20 g is injected with 120-600 μg taurine.