CN115337709A - Purification and filtration process for biological pharmacy - Google Patents
Purification and filtration process for biological pharmacy Download PDFInfo
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- CN115337709A CN115337709A CN202210985521.1A CN202210985521A CN115337709A CN 115337709 A CN115337709 A CN 115337709A CN 202210985521 A CN202210985521 A CN 202210985521A CN 115337709 A CN115337709 A CN 115337709A
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Abstract
The invention relates to the technical field of biological pharmacy, and discloses a purification and filtration process for biological pharmacy, which comprises the following steps: s1, degreasing raw materials, S2, precipitating protein, S3, filtering and removing impurities, S4, carrying out ultrafiltration processing, and S5, separating and purifying. The invention filters the material step by arranging the metal filter screen, the microporous filter membrane and the ultrafiltration device, can effectively remove various impurities, and can obtain the material with higher purity by processing the material by adopting ultrafiltration and ion exchange chromatography.
Description
Technical Field
The invention relates to the technical field of biological pharmacy, in particular to a purification and filtration process for biological pharmacy.
Background
The biological medicine is a product for prevention, treatment and diagnosis which is manufactured by comprehensively utilizing the principles and methods of science such as microbiology, chemistry, biochemistry, biotechnology, pharmacy and the like from organisms, biological tissues, cells, organs, body fluids and the like by applying the research results of microbiology, biology, medicine, biochemistry and the like. In the production process of biopharmaceuticals, purification is an important process. In brief, the purification is to remove impurities in the product and improve the purity of the product.
Lactoferrin is a multifunctional glycoprotein, and has various biological activities, such as antiviral, anticancer, antioxidant, and immunity regulating effects. Plays an important role in disease prevention and treatment, nutrition supplement, food and drug development and the like. The existing methods for extracting and purifying lactoferrin mainly comprise two methods: the method is low in cost, but low in production efficiency, and is not suitable for large-scale production; the other method is ultrafiltration, which separates substances with different molecular weights according to the pore sizes of membranes with different sizes, and has high production efficiency but low product purity.
Disclosure of Invention
The invention provides a purification and filtration process for biopharmaceuticals, which has the beneficial effect of higher purity and solves the problem that the ultrafiltration method mentioned in the background technology has higher production efficiency but lower product purity.
The invention provides the following technical scheme: a purification and filtration process for biopharmaceuticals, comprising the steps of:
s1, raw material degreasing: weighing a certain amount of bovine colostrum raw material, filtering the raw material through a coarse filtering device to remove large-volume impurities in the raw material, adding the filtered raw material into a centrifugal tube, placing the centrifugal tube in a high-speed refrigerated centrifuge, and performing centrifugal treatment for 0.5-1 hour at the temperature of 2-5 ℃ and the rotating speed of 8000-10000 r/min to remove upper fat in the raw material;
s2, protein precipitation: adding 1-2moL/L dilute sulfuric acid into the degreased raw material, uniformly stirring, heating in a water bath to 35-40 ℃, preserving heat for 30 minutes, putting the raw material into a high-speed freezing centrifuge again, centrifuging, and taking upper layer whey protein after centrifuging;
s3, filtering and removing impurities: placing the upper layer whey protein solution at a filtering device, and filtering the upper layer whey protein solution by adopting a microporous filter membrane with the specification of 0.45 micron to remove dust and bacteria in the solution;
s4, ultrafiltration processing: placing the solution into a tangential flow ultrafiltration device, adding distilled water to dilute the solution, wherein the ratio of the distilled water to the solution is 1:1, filtering the solution at the temperature of 34-38 ℃, the pressure of 0.2-0.5MPa and the pH value of 7, and collecting a permeate;
s5, separation and purification: and (3) separating and purifying the permeate by adopting cation exchange chromatography, eluting by using a phosphate buffer solution with the pH value of 6.9 and a sodium chloride solution, and collecting a product, thereby obtaining the high-purity lactoferrin solution.
As an alternative to the purification filtration process for biopharmaceuticals of the present invention, wherein: the coarse filtering device in the step S1 comprises a shell, a rotating seat is arranged in the shell, a driving motor used for driving the rotating seat to rotate is arranged at the lower end of the rotating seat, a filtering cylinder is arranged at the upper end of the rotating seat, a plurality of fixing grooves are formed in the filtering cylinder, and filtering assemblies are arranged in the fixing grooves;
the filter assembly comprises a filter plate, the filter plate is connected with a filter cartridge in a sliding mode, a first spring is arranged on one side of the filter plate, a fixing seat is arranged at the middle of the filter cartridge, a plurality of fixing plates are arranged on the fixing seat, the positions and the number of the fixing plates correspond to the fixing grooves one to one, and a plurality of ejector pins are arranged on the fixing plates.
As an alternative to the purification filtration process for biopharmaceuticals of the present invention, wherein: the filter plate is characterized in that first scraping plates are symmetrically arranged on the other side of the filter plate and are elastically connected with the filter plate through second springs, fixing columns are arranged at the lower ends of the first scraping plates, inclined bottoms are arranged at the lower ends of the fixing columns, and the fixing columns are elastically connected with the first scraping plates through third springs.
As an alternative to the purification filtration process for biopharmaceuticals of the present invention, wherein: be equipped with drive assembly in the roating seat, just drive assembly includes the movable block that the symmetry set up, one side of movable block is equipped with the inclined plane, just the movable block pass through the fourth spring with roating seat elastic connection, the upper end of movable block be equipped with the draw-in groove that the fixed column agrees with mutually.
As an alternative to the purification and filtration process for biopharmaceutical use of the present invention, wherein: the one end of draw-in groove is equipped with the stopper, the one end of stopper is equipped with the fixed axle, just the fixed axle pass through the torsional spring with movable block elastic connection, one side of movable block be equipped with be used for right the stopper carries out the magnetism of fixing and inhales the piece.
As an alternative to the purification filtration process for biopharmaceuticals of the present invention, wherein: the rotary seat is provided with a plurality of electric push rods, the positions and the number of the electric push rods correspond to the fixing grooves one by one, the electric push rods are fixedly connected with the rotary seat, and push plates are arranged on piston rods of the electric push rods.
As an alternative to the purification filtration process for biopharmaceuticals of the present invention, wherein: one side that the filter kept away from first scraper blade is equipped with the second scraper blade, just the one end of second scraper blade with movable block fixed connection, the lower extreme of filter is equipped with the ejector pin.
As an alternative to the purification filtration process for biopharmaceuticals of the present invention, wherein: the movable block is provided with a movable latch, and one end of the movable latch is elastically connected with the movable block through a fifth spring.
As an alternative to the purification filtration process for biopharmaceuticals of the present invention, wherein: one side of the filter plate is provided with a movable latch limiting mechanism, the movable latch limiting mechanism comprises a fixing frame, the fixing frame is n-shaped, clamping blocks are arranged at two ends of the fixing frame, and the fixing frame is elastically connected with the filter cartridge through a sixth spring.
As an alternative to the purification filtration process for biopharmaceuticals of the present invention, wherein: the upper end of cartridge filter is equipped with the inlet pipe, just the inlet pipe pass through universal swivel joint with the cartridge filter intercommunication, the outside of cartridge filter is equipped with a plurality of opening, just the opening with the fixed slot intercommunication, the opening part is equipped with the filter screen.
The invention has the following beneficial effects:
1. this a purification filtration process for bio-pharmaceuticals, this technique carries out coarse filtration to the raw materials through metal filter screen to get rid of the bulky impurity in the raw materials, then carry out centrifugal degreasing to it, carry out the albumen sediment after that, then come to filter the product through microporous membrane, rethread ultrafiltration device filters the material after that, because the purity of the material after the ultrafiltration is not high, this technical scheme purifies it through ion exchange chromatogram again, comes further to improve the purity of product. The invention filters the material step by arranging the metal filter screen, the microporous filter membrane and the ultrafiltration device, can effectively remove various impurities, and can obtain the material with higher purity by processing the material by adopting ultrafiltration and ion exchange chromatography.
2. According to the purifying and filtering process for the bio-pharmaceuticals, the coarse filtering device with the filter cylinder is arranged, the filter cylinder is internally provided with the filter plate, the filter cylinder can rotate along with the rotating seat, when materials enter the filter cylinder, the materials can be thrown out due to the rotation of the filter cylinder, the materials can be filtered through the filter plate in the throwing-out process, the filter plate is movably arranged in the filter cylinder, the filter cylinder is also internally provided with the fixed plate, the fixed plate is provided with the ejector pins, and the ejector pins are inserted into the filter holes under the normal condition; when the filter cylinder rotates, the filter plate can be thrown out under the action of centrifugal force, at the moment, the filter plate moves towards the direction far away from the fixed plate, and the ejector pin is separated from contact with the filter plate;
when needs clear up the filter, only need halt temporarily earlier, when the cartridge filter is no longer rotated, the filter can return the normal position under the effect of first spring, and at this moment, the thimble can be pegged graft on the filter once more to the completion is to the clearance of filtration pore, then start once more, filter can, cleaning efficiency is higher.
3. This a purification filtration technology for bio-pharmaceuticals, one side through at the filter sets up first scraper blade, first scraper blade is mobilizable, when the filter is thrown away under the effect of centrifugal force, first scraper blade and fixed column motion can be taken to the filter, when moving to movable block department, first scraper blade passes through fixed column and movable block joint, when chinese angelica needs clear up the filter, only need start electric putter, electric putter can promote the push pedal motion, the one end and the movable block of push pedal are contradicted, because be equipped with the inclined plane on the movable block, under the promotion of push pedal, the movable block opens, the movable block is when the motion, can take first scraper blade to move together, first scraper blade is when the motion, can scrape down the debris of filter one side, thereby accomplish the clearance to the filter, the clearance effect is better.
Drawings
FIG. 1 is a process flow diagram of the present invention.
FIG. 2 is a schematic view of the coarse filter device according to the present invention.
Fig. 3 is a schematic view of the internal structure of the filter cartridge of the present invention.
Fig. 4 is a front view of the filter plate of the present invention.
FIG. 5 is a schematic view of the back side structure of the filter plate of the present invention.
Fig. 6 is a schematic diagram of a movable block structure according to the present invention.
Fig. 7 is a schematic view of the filter cartridge structure of the present invention.
In the figure: 1. a housing; 2. a rotating base; 3. a drive motor; 4. a filter cartridge; 5. fixing grooves; 6. a filter plate; 7. a first spring; 8. a fixed seat; 9. a fixing plate; 10. a thimble; 11. a first squeegee; 12. a second spring; 13. fixing a column; 14. a third spring; 15. a movable block; 16. a fourth spring; 17. a card slot; 18. a limiting block; 19. a fixed shaft; 20. a torsion spring; 21. a magnetic attraction block; 22. an electric push rod; 23. pushing a plate; 24. a second squeegee; 25. a top rod; 26. a movable latch; 27. a fifth spring; 28. a fixed mount; 29. a clamping block; 30. a sixth spring; 31. a feed pipe; 32. and (4) opening.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Example 1
Referring to fig. 1, the present invention provides a purification and filtration process for biopharmaceuticals, comprising the steps of:
s1, raw material degreasing: weighing a certain amount of bovine colostrum raw material, filtering the raw material through a coarse filtering device to remove large-volume impurities in the raw material, adding the filtered raw material into a centrifugal tube, placing the centrifugal tube in a high-speed refrigerated centrifuge, and performing centrifugal treatment for 0.5-1 hour at the temperature of 2-5 ℃ and the rotating speed of 8000-10000 r/min to remove upper fat in the raw material;
s2, protein precipitation: adding 1-2moL/L dilute sulfuric acid into the degreased raw material, uniformly stirring, heating in a water bath to 35-40 ℃, preserving heat for 30 minutes, putting the raw material into a high-speed freezing centrifuge again, centrifuging, and taking upper layer whey protein after centrifuging;
s3, filtering and removing impurities: placing the upper layer whey protein solution at a filtering device, and filtering the upper layer whey protein solution by adopting a microporous filter membrane with the specification of 0.45 micron to remove dust and bacteria in the solution;
s4, ultrafiltration processing: placing the solution into a tangential flow ultrafiltration device, adding distilled water to dilute the solution, wherein the ratio of the distilled water to the solution is 1:1, filtering the solution at the temperature of 34-38 ℃, the pressure of 0.2-0.5MPa and the pH value of 7, and collecting a permeate;
s5, separation and purification: and separating and purifying the permeate by adopting cation exchange chromatography, eluting by using a phosphate buffer solution with the pH value of 6.9 and a sodium chloride solution, and collecting a product to obtain the high-purity lactoferrin solution.
This technique comes to carry out the coarse filtration to the raw materials through metal filter screen to get rid of the bulky impurity in the raw materials, then carry out the centrifugation degrease to it, carry out the albumen sediment after that, then come to filter the product through microporous filter membrane, rethread ultrafiltration device filters the material after that, because the purity of the material after the ultrafiltration is not high, this technical scheme purifies it through the ion exchange chromatogram again, further improves the purity of product. The invention filters the material step by arranging the metal filter screen, the microporous filter membrane and the ultrafiltration device, can effectively remove various impurities, and can obtain the material with higher purity by processing the material by adopting ultrafiltration and ion exchange chromatography.
Example 2
The present embodiment is explained based on embodiment 1, and specifically, referring to fig. 1 to 7, the coarse filtering device in step S1 includes a housing 1, a rotating base 2 is disposed in the housing 1, a driving motor 3 for driving the rotating base 2 to rotate is disposed at a lower end of the rotating base 2, a filter cartridge 4 is disposed at an upper end of the rotating base 2, a plurality of fixing slots 5 are disposed on the filter cartridge 4, and a filtering assembly is disposed in the fixing slots 5;
the filter assembly comprises a filter 6, the filter 6 is connected with a filter cartridge 4 in a sliding mode, one side of the filter 6 is provided with a first spring 7, the middle of the filter cartridge 4 is provided with a fixing seat 8, a plurality of fixing plates 9 are arranged on the fixing seat 8, the positions and the number of the fixing plates 9 correspond to the fixing grooves 5 one to one, and a plurality of ejector pins 10 are arranged on the fixing plates 9.
In the process of production, coarse filtration device is blockked up by debris easily, for guaranteeing machining efficiency, needs regularly clear up coarse filtration device, and current clearance scheme is generally the opening device shell, takes out the filter, or directly clears up the filter, and its clearance efficiency is lower, and serious influence machining efficiency for this reason, this technical scheme has still improved coarse filtration device.
The coarse filtering device comprises a shell 1, a rotary seat 2 and a filter cylinder 4 are arranged in the shell 1, a filter plate 6 is arranged in the filter cylinder 4, the filter cylinder 4 can rotate along with the rotary seat 2, when materials enter the filter cylinder 4, the materials can be thrown out due to the rotation of the filter cylinder 4, the materials can be filtered through the filter plate 6 in the throwing-out process, the filter plate 6 can be movably arranged in the filter cylinder 4, a fixing plate 9 is also arranged in the filter cylinder 4, a plurality of thimbles 10 are arranged on the fixing plate 9, the thimbles 10 are in one-to-one correspondence with filter holes on the filter plate 6,
under the normal condition, filter 6 can be drawn close to fixed plate 9 orientation under the promotion of first spring 7, at this moment, thimble 10 is pegged graft in the filter, when cartridge filter 4 rotates, filter 6 can be thrown away under the effect of centrifugal force, at this moment, filter 6 is to the direction motion of keeping away from fixed plate 9, thimble 10 and filter 6 break away from the contact, when needs clear up filter 6, only need temporary stop earlier, when cartridge filter 4 no longer rotates, filter 6 can return the normal position under the effect of first spring 7, at this moment, thimble 10 can peg graft once more on filter 6, thereby accomplish the clearance to the filter orifice, then start once more, filter can, the cleaning efficiency is higher.
Example 3
The present embodiment is explained based on embodiment 1, and specifically, referring to fig. 1 to 7, a first scraper 11 is symmetrically disposed on the other side of the filter plate 6, the first scraper 11 is elastically connected to the filter plate 6 through a second spring 12, a fixing column 13 is disposed at the lower end of the first scraper 11, an inclined bottom is disposed at the lower end of the fixing column 13, and the fixing column 13 is elastically connected to the first scraper 11 through a third spring 14.
Be equipped with drive assembly in roating seat 2, and drive assembly is equipped with the inclined plane including the movable block 15 that the symmetry set up, one side of movable block 15, and movable block 15 passes through fourth spring 16 and 2 elastic connection of roating seat, and the upper end of movable block 15 is equipped with the draw-in groove 17 that agrees with mutually with fixed column 13.
One end of the clamping groove 17 is provided with a limiting block 18, one end of the limiting block 18 is provided with a fixed shaft 19, the fixed shaft 19 is elastically connected with the movable block 15 through a torsion spring 20, and one side of the movable block 15 is provided with a magnetic attraction block 21 for fixing the limiting block 18. Be equipped with a plurality of electric putter 22 on the roating seat 2, and electric putter 22's position and quantity and fixed slot 5 one-to-one, electric putter 22 and 2 fixed connection of roating seat, and be equipped with push pedal 23 on electric putter 22's the piston rod.
In order to improve the cleaning effect of the filter plate 6, according to the technical scheme, two first scraper blades 11 are arranged on one side of the filter plate 6, the first scraper blades 11 are elastically connected with the filter plate 6 through second springs 12, meanwhile, a transmission assembly matched with the first scraper blades 11 is arranged in the rotary seat 2, the transmission assembly comprises a movable block 15, a movable fixed column 13 is arranged on the lower side of the first scraper blades 11, a clamping groove 17 matched with the fixed column 13 is formed in the movable block 15, when the filter plate 6 is thrown out under the action of centrifugal force, the filter plate 6 can drive the first scraper blades 11 and the fixed column 13 to move, when the filter plate moves to the movable block 15, the bottom end of the fixed column 13 is abutted against the movable block 15, the fixed column 13 can overcome the resistance of a third spring 14 to move upwards and then is inserted into the clamping groove 17, and at the moment, the fixed column 13 and the movable block 15 are in a clamping state;
when the chinese angelica needs to clear up filter 6, only need start electric putter 22, electric putter 22 can promote push pedal 23 motion, push pedal 23's one end is contradicted with movable block 15, because be equipped with the inclined plane on the movable block 15, under the promotion of push pedal 23, movable block 15 opens, movable block 15 is when moving, can take first scraper blade 11 to move together, first scraper blade 11 is when moving, can scrape the debris of filter 6 one side down, thereby accomplish the clearance to filter 6, the clearance effect is better.
One end of the clamping groove 17 is of an open structure, a limiting block 18 used for plugging the clamping groove 17 is arranged on one side of the movable block 15, one end of the limiting block 18 is rotatably connected with the movable block 15 through a fixed shaft 19, the other end of the limiting block is adsorbed on a magnetic suction block 21 on the movable block 15, when the movable block 15 outwards pulls the fixed column 13, the fixed column 13 can be abutted against the limiting block 18, the limiting block 18 exists, the fixed column 13 can not be separated from the clamping groove 17, the first scraper 11 can not move after moving to a certain position, at the moment, the limiting block 18 can overcome the suction force of the magnetic suction block 21 and rotate under the abutting of the fixed column 13, then the limiting block 18 is separated from the fixed column 13 and returns to the original position under the action of the torsion spring 20, and at the moment, the movable block 15 is not clamped with the fixed column 13.
Example 4
The present embodiment is explained based on embodiment 1, specifically, referring to fig. 1 to fig. 7, a second scraper 24 is disposed on one side of the filter plate 6 away from the first scraper 11, one end of the second scraper 24 is fixedly connected to the movable block 15, and a push rod 25 is disposed at the lower end of the filter plate 6.
The movable block 15 is provided with a movable latch 26, and one end of the movable latch 26 is elastically connected with the movable block 15 through a fifth spring 27.
In order to further improve the cleaning effect of the filter plate 6, the other side of the filter plate 6 is provided with a second scraper 24, the second scraper 24 is fixed on the movable block 15, when the push plate 23 pushes the movable block 15 open, the movable block 15 can be in place linkage with the movable latch limiting mechanism and is always in an open state under the action of the movable latch limiting mechanism, when the filter cartridge 4 does not rotate any more, the filter plate 6 can return to the original position, when the filter cartridge 4 rotates again, the filter plate 6 can move towards the movable block 15 under the action of centrifugal force, the lower end of the filter plate 6 is provided with a mandril 25, the mandril 25 can firstly abut against the movable latch limiting mechanism, so that the limitation of the movable latch limiting mechanism on the movable block 15 is removed, then the movable block 15 returns to the original position under the action of the fourth spring 16, in the process of returning to the original position, the second scraper 24 can be driven to move, the other side of the filter plate 6 can be cleaned through the second scraper 24, and the cleaning effect is better.
Example 5
The present embodiment is explained based on embodiment 1, and specifically, referring to fig. 1 to 7, a movable latch limiting mechanism is disposed on one side of the filter plate 6, the movable latch limiting mechanism includes a fixed frame 28, the fixed frame 28 is n-shaped, two ends of the fixed frame 28 are both provided with a latch 29, and the fixed frame 28 is elastically connected to the filter cartridge 4 through a sixth spring 30.
The movable block is provided with a movable latch 26, the fixed frame 28 is provided with a fixture block 29, when the movable block 15 is pushed open by the push plate 23, the movable latch 26 abuts against the fixture block 29, the movable latch 26 retracts into the movable block 15 under the push of the fixture block 29, and then returns to the original position under the action of the fifth spring 27, at the moment, the movable latch 26 and the fixture block 29 are clamped together, so that the movable block 15 cannot move any more, when the push rod 25 abuts against the fixed frame 28, the fixed frame and the fixture block 29 are pushed to move, the fixture block 29 is staggered with the movable latch 26, and then the movable block 15 can return to the original position under the action of the fourth spring 16.
Example 6
The present embodiment is explained based on embodiment 1, specifically, referring to fig. 1 to fig. 7, a feeding pipe 31 is disposed at an upper end of the filter cartridge 4, the feeding pipe 31 is communicated with the filter cartridge 4 through a universal rotary pipe joint, a plurality of openings 32 are disposed at an outer side of the filter cartridge 4, the openings 32 are communicated with the fixing groove 5, and a filter screen is disposed at the openings 32.
In the material passed through inlet pipe 31 entering cartridge filter 4, be equipped with a plurality of opening 32 in the outside of cartridge filter 4, the material is back in entering cartridge filter 4, can be in proper order through fixed slot 5, filter 6 then flow through opening 32, and this technical scheme mainly filters the material through filter 6, and the filter screen of opening 32 department is as the replenishment of filter 6.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the technical principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (10)
1. A purification and filtration process for biopharmaceuticals, characterized in that: the method comprises the following steps:
s1, raw material degreasing: weighing a certain amount of bovine colostrum raw material, filtering the raw material through a coarse filtering device to remove large-volume impurities in the raw material, adding the filtered raw material into a centrifugal tube, placing the centrifugal tube in a high-speed refrigerated centrifuge, and performing centrifugal treatment for 0.5-1 hour at the temperature of 2-5 ℃ and the rotating speed of 8000-10000 r/min to remove upper fat in the raw material;
s2, protein precipitation: adding 1-2moL/L dilute sulfuric acid into the degreased raw material, uniformly stirring, heating in a water bath to 35-40 ℃, preserving heat for 30 minutes, putting the raw material into a high-speed freezing centrifuge again, centrifuging, and taking upper-layer whey protein after centrifuging;
s3, filtering and removing impurities: placing the upper layer whey protein solution at a filtering device, and filtering the upper layer whey protein solution by adopting a microporous filter membrane with the specification of 0.45 micron to remove dust and bacteria in the solution;
s4, ultrafiltration processing: placing the solution into a tangential flow ultrafiltration device, adding distilled water to dilute the solution, wherein the ratio of the distilled water to the solution is 1:1, filtering the solution at the temperature of 34-38 ℃, the pressure of 0.2-0.5MPa and the pH value of 7, and collecting a permeate;
s5, separation and purification: and (3) separating and purifying the permeate by adopting cation exchange chromatography, eluting by using a phosphate buffer solution with the pH value of 6.9 and a sodium chloride solution, and collecting a product, thereby obtaining the high-purity lactoferrin solution.
2. The purification and filtration process for biopharmaceuticals of claim 1, wherein: the coarse filtering device in the step S1 comprises a shell (1), a rotating base (2) is arranged in the shell (1), a driving motor (3) used for driving the rotating base (2) to rotate is arranged at the lower end of the rotating base (2), a filtering cylinder (4) is arranged at the upper end of the rotating base (2), a plurality of fixing grooves (5) are formed in the filtering cylinder (4), and filtering components are arranged in the fixing grooves (5);
the filter assembly comprises a filter plate (6), the filter plate (6) is connected with a filter cartridge (4) in a sliding mode, a first spring (7) is arranged on one side of the filter plate (6), a fixing seat (8) is arranged in the middle of the filter cartridge (4), a plurality of fixing plates (9) are arranged on the fixing seat (8), the positions and the number of the fixing plates (9) correspond to the fixing grooves (5) one to one, and a plurality of ejector pins (10) are arranged on the fixing plates (9).
3. A purification and filtration process for biopharmaceuticals, according to claim 2, characterized in that: the filter plate is characterized in that first scraping plates (11) are symmetrically arranged on the other side of the filter plate (6), the first scraping plates (11) are elastically connected with the filter plate (6) through second springs (12), fixing columns (13) are arranged at the lower ends of the first scraping plates (11), inclined bottoms are arranged at the lower ends of the fixing columns (13), and the fixing columns (13) are elastically connected with the first scraping plates (11) through third springs (14).
4. A purification and filtration process for biopharmaceutical production according to claim 3, wherein said filtration process comprises the steps of: be equipped with drive assembly in roating seat (2), just drive assembly includes movable block (15) that the symmetry set up, one side of movable block (15) is equipped with the inclined plane, just movable block (15) through fourth spring (16) with roating seat (2) elastic connection, the upper end of movable block (15) be equipped with draw-in groove (17) that fixed column (13) agree with mutually.
5. The purification and filtration process for biopharmaceuticals according to claim 4, wherein said filtration process comprises: the one end of draw-in groove (17) is equipped with stopper (18), the one end of stopper (18) is equipped with fixed axle (19), just fixed axle (19) through torsional spring (20) with movable block (15) elastic connection, one side of movable block (15) be equipped with be used for right stopper (18) carry out the magnetism of fixing and inhale piece (21).
6. A purification and filtration process for biopharmaceuticals, according to claim 2, characterized in that: be equipped with a plurality of electric putter (22) on roating seat (2), just the position and the quantity of electric putter (22) with fixed slot (5) one-to-one, electric putter (22) with roating seat (2) fixed connection, just be equipped with push pedal (23) on the piston rod of electric putter (22).
7. A purification and filtration process for biopharmaceuticals, according to claim 3, wherein said filtration process comprises: keep away from in filter (6) one side of first scraper blade (11) is equipped with second scraper blade (24), just the one end of second scraper blade (24) with movable block (15) fixed connection, the lower extreme of filter (6) is equipped with ejector pin (25).
8. The purification and filtration process for biopharmaceuticals according to claim 4, wherein said filtration process comprises: be equipped with movable latch (26) on movable block (15), just the one end of activity latch (26) through fifth spring (27) with movable block (15) elastic connection.
9. The purification and filtration process for biopharmaceuticals according to claim 8, wherein said filtration process comprises: one side of filter (6) is equipped with movable latch stop gear, just movable latch stop gear includes mount (28), mount (28) are n shape, just the both ends of mount (28) all are equipped with fixture block (29), mount (28) through sixth spring (30) with cartridge filter (4) elastic connection.
10. A purification and filtration process for biopharmaceuticals, according to claim 2, characterized in that: the upper end of cartridge filter (4) is equipped with inlet pipe (31), just inlet pipe (31) through universal swivel joint with cartridge filter (4) intercommunication, the outside of cartridge filter (4) is equipped with a plurality of opening (32), just opening (32) with fixed slot (5) intercommunication, opening (32) department is equipped with the filter screen.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210985521.1A CN115337709B (en) | 2022-08-17 | 2022-08-17 | Purification and filtration process for biopharmaceuticals |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210985521.1A CN115337709B (en) | 2022-08-17 | 2022-08-17 | Purification and filtration process for biopharmaceuticals |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115337709A true CN115337709A (en) | 2022-11-15 |
| CN115337709B CN115337709B (en) | 2023-09-15 |
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| GB896474A (en) * | 1958-07-31 | 1962-05-16 | Gen Motors Corp | Improvements in and relating to pumps |
| CN101724013A (en) * | 2008-10-24 | 2010-06-09 | 哈尔滨康普乳品有限公司 | Method for separating and purifying immunoglobulin A, immunoglobulin G and lactoferrin from bovine colostrum in industrializing way |
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| CN212284850U (en) * | 2020-03-26 | 2021-01-05 | 南昌大学 | Be used for bio-pharmaceuticals separation and purification device |
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| CN115337709B (en) | 2023-09-15 |
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