CN115327745A - High-flux flat field apochromatic objective lens - Google Patents
High-flux flat field apochromatic objective lens Download PDFInfo
- Publication number
- CN115327745A CN115327745A CN202211005330.0A CN202211005330A CN115327745A CN 115327745 A CN115327745 A CN 115327745A CN 202211005330 A CN202211005330 A CN 202211005330A CN 115327745 A CN115327745 A CN 115327745A
- Authority
- CN
- China
- Prior art keywords
- lens
- focal length
- cemented
- denotes
- meniscus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 230000005499 meniscus Effects 0.000 claims abstract description 38
- 238000012163 sequencing technique Methods 0.000 abstract description 14
- 238000003384 imaging method Methods 0.000 abstract description 13
- 230000004907 flux Effects 0.000 abstract description 6
- 230000000007 visual effect Effects 0.000 abstract description 3
- 230000004075 alteration Effects 0.000 description 15
- 206010010071 Coma Diseases 0.000 description 4
- 238000010586 diagram Methods 0.000 description 4
- 230000003287 optical effect Effects 0.000 description 4
- 201000009310 astigmatism Diseases 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000013459 approach Methods 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000006059 cover glass Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B13/00—Optical objectives specially designed for the purposes specified below
- G02B13/001—Miniaturised objectives for electronic devices, e.g. portable telephones, webcams, PDAs, small digital cameras
- G02B13/0055—Miniaturised objectives for electronic devices, e.g. portable telephones, webcams, PDAs, small digital cameras employing a special optical element
- G02B13/006—Miniaturised objectives for electronic devices, e.g. portable telephones, webcams, PDAs, small digital cameras employing a special optical element at least one element being a compound optical element, e.g. cemented elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Abstract
The invention discloses a high-flux flat field apochromatic objective lens which comprises a first lens group, a second lens group and a third lens group which are coaxially arranged in sequence from an object side to an image side, wherein the first lens group comprises a first meniscus lens and a second meniscus lens; the second lens group comprises a first cemented lens consisting of a first biconvex lens, a first biconcave lens and a second biconvex lens, a third biconvex lens with positive focal power, and a second cemented lens consisting of a third meniscus lens and a fourth biconvex lens; the third lens group includes a third cemented lens composed of a fourth meniscus lens and a fifth meniscus lens, and a fourth cemented lens composed of a second biconcave lens and a fifth biconvex lens. The embodiment of the invention can simultaneously increase the imaging visual field and the numerical aperture, improve the sequencing flux and can be widely applied to the technical field of optics.
Description
Technical Field
The invention relates to the technical field of optics, in particular to a high-flux flat field apochromatic objective lens.
Background
Sequencing flux is one of core indexes of a gene sequencer and reflects data output quantity in unit time; the output time of the detection result is influenced by the magnitude of the sequencing flux, and the sequencing cost is also related. In a sequencer, the core optical device influencing sequencing flux is a microscope objective. There are two core parameters in the microscope objective: one is the numerical aperture, and the parameter determines the imaging resolution of the objective lens, and the size of the imaging resolution determines the density of the DNA on the sequencing chip; the other is an imaging field of view, the larger the field of view is, the larger the range of one shooting is, and the shorter the time for completing the scanning imaging of the sequencing chip is. It follows that high numerical aperture and large field objective lenses are key to increasing sequencing throughput.
The imaging field of view and the numerical aperture are two key parameters for determining sequencing throughput, but the two parameters are usually balanced, namely the field of view of the large-numerical-aperture objective lens is smaller, and the numerical aperture of the large-field objective lens is smaller, so that the large imaging field of view and the high definition cannot be simultaneously considered.
Disclosure of Invention
In view of this, an object of the embodiments of the present invention is to provide a high-throughput flat field apochromatic objective lens, which can simultaneously achieve a large imaging field and a large numerical aperture, and improve sequencing throughput.
The embodiment of the invention provides a high-flux flat field apochromatic objective lens which comprises a first lens group, a second lens group and a third lens group which are coaxially arranged in sequence from an object space to an image space, wherein,
the first lens group comprises a first meniscus lens and a second meniscus lens which are sequentially arranged;
the second lens group comprises a first biconvex lens, a first biconcave lens, a second biconvex lens, a third meniscus lens and a fourth biconvex lens which are sequentially arranged, wherein the first biconvex lens, the first biconcave lens and the second biconvex lens form a first cemented lens, and the third meniscus lens and the fourth biconvex lens form a second cemented lens;
the third lens group comprises a fourth meniscus lens, a fifth meniscus lens, a second biconcave lens and a fifth biconvex lens which are arranged in sequence, the fourth meniscus lens and the fifth meniscus lens form a third cemented lens, and the second biconcave lens and the fifth biconvex lens form a fourth cemented lens.
Optionally, a focal length of the first lens group satisfies the following relation:
10.2<f L23 /f<11
wherein f is L23 Denotes a focal length of the first lens group, and f denotes a focal length of the objective lens.
Optionally, the focal length of the first cemented lens satisfies the following relation:
6.42<f L456 /f<7.15
wherein, f L456 Denotes a focal length of the first cemented lens, and f denotes a focal length of the objective lens.
Optionally, a focal length of the third lenticular lens satisfies the following relation:
2.91<f L7 /f<3.32
wherein, f L7 Denotes a focal length of the third lenticular lens, and f denotes a focal length of the objective lens.
Optionally, the focal length of the second cemented lens satisfies the following relation:
10.6<f L89 /f<12.3
wherein, f L89 Denotes a focal length of the second cemented lens, and f denotes a focal length of the objective lens.
Optionally, the focal length of the third cemented lens satisfies the following relation:
-6.01<f L1011 /f<-5.66
wherein f is L1011 Denotes a focal length of the third cemented lens, and f denotes a focal length of the objective lens.
Optionally, the focal length of the fourth cemented lens satisfies the following relation:
-69.1<f L1213 /f<-70.2
wherein f is L1213 Denotes a focal length of the fourth cemented lens, and f denotes a focal length of the objective lens.
Optionally, all lenses of the first lens group, the second lens group, and the third lens group are spherical lenses.
The implementation of the embodiment of the invention has the following beneficial effects: the objective lens in the embodiment comprises three lens groups coaxially arranged from an object side to an image side, and the first lens group comprises two meniscus lenses; the second lens group includes two cemented lenses and a double convex lens having an intermediate refractive power, wherein the first cemented lens composed of two double convex lenses and a double concave lens and the second cemented lens composed of a meniscus lens and a double convex lens; the third lens group comprises two cemented lenses, wherein the third cemented lens is composed of two meniscus lenses, and the fourth cemented lens is composed of a biconcave lens and a biconvex lens; the light emitted by the object to be detected firstly passes through the first lens group to reduce the aperture angle, then the second lens group corrects one or more of spherical aberration, coma aberration or chromatic aberration, and then the third lens group eliminates one or more of field curvature, astigmatism or chromatic aberration, so that the imaging visual field and the numerical aperture are simultaneously increased, and the sequencing flux is improved.
Drawings
FIG. 1 is a schematic structural diagram of a high-throughput field apochromatic objective lens provided by an embodiment of the present invention;
FIG. 2 is a MTF curve of a high-throughput apochromatic objective lens provided by an embodiment of the present invention;
FIG. 3 is a dot-sequence diagram of a high-throughput field-flattened apochromatic objective lens provided by an embodiment of the present invention;
FIG. 4 is a graph of field curvature and distortion for a high throughput field-flattened apochromatic objective lens provided by an embodiment of the present invention;
fig. 5 is a diffraction energy distribution curve of a high-throughput apochromatic objective lens according to an embodiment of the present invention.
Detailed Description
The invention is described in further detail below with reference to the figures and the specific embodiments. The step numbers in the following embodiments are provided only for convenience of illustration, the order between the steps is not limited at all, and the execution order of each step in the embodiments can be adapted according to the understanding of those skilled in the art.
As shown in fig. 1, an embodiment of the present invention provides a high-throughput flat field apochromatic objective lens, which includes a first lens group G1, a second lens group G2, and a third lens group G3 coaxially arranged in order from an object side to an image side, wherein,
the first lens group G1 includes a first meniscus lens L2 and a second meniscus lens L3 which are sequentially disposed;
the second lens group G2 includes a first biconvex lens L4, a first biconcave lens L5, a second biconvex lens L6, a third biconvex lens L7, a third meniscus lens L8 and a fourth biconvex lens L9, which are sequentially disposed, the first biconvex lens L4, the first biconcave lens L5 and the second biconvex lens L6 are combined into a first cemented lens, and the third meniscus lens L8 and the fourth biconvex lens L9 constitute a second cemented lens;
the third lens group G3 includes a fourth meniscus lens L10, a fifth meniscus lens L11, a second biconcave lens L12, and a fifth biconvex lens L13, which are sequentially disposed, the fourth meniscus lens L10 and the fifth meniscus lens L11 constitute a third cemented lens, and the second biconcave lens L12 and the fifth biconvex lens L13 constitute a fourth cemented lens.
Specifically, L1 is a cover glass of the sample, and may also be glass on the upper layer of the flow channel of the sequencing chip. The first lens group G1 comprises L2 and L3, and the first lens group forms a front collimating surface, collects a large divergence angle optical signal and converts the large divergence angle optical signal into a small angle optical signal, so that the numerical aperture is effectively increased, and excessive spherical aberration and/or coma aberration is avoided. The second lens group G2 comprises lenses L4-L9, and the second lens group is used for correcting spherical aberration, coma aberration and chromatic aberration. The third lens group G3 comprises lenses L10-L13 for eliminating curvature of field, astigmatism and chromatic aberration, wherein L11 is a thick meniscus lens.
Optionally, a focal length of the first lens group satisfies the following relation:
10.2<f L23 /f<11
wherein, f L23 Denotes a focal length of the first lens group, and f denotes a focal length of the objective lens.
Specifically, see FIG. 1,f L23 Denotes a focal length of the first lens group formed by the lenses L2 and L3.
Optionally, the focal length of the first cemented lens satisfies the following relation:
6.42<f L456 /f<7.15
wherein f is L456 Denotes a focal length of the first cemented lens, and f denotes a focal length of the objective lens.
Specifically, see FIG. 1,f L456 Denotes the focal length of the first cemented lens formed by lenses L4, L5, and L6.
Optionally, a focal length of the third lenticular lens satisfies the following relation:
2.91<f L7 /f<3.32
wherein f is L7 Denotes a focal length of the third lenticular lens, and f denotes a focal length of the objective lens.
Specifically, see FIG. 1,f L7 Indicating the focal length of lens L7.
Optionally, the focal length of the second cemented lens satisfies the following relation:
10.6<f L89 /f<12.3
wherein, f L89 Denotes a focal length of the second cemented lens, and f denotes a focal length of the objective lens.
Specifically, see FIG. 1,f L89 Indicating the focal length of the second cemented lens formed by lenses L8 and L9.
Optionally, the focal length of the third cemented lens satisfies the following relation:
-6.01<f L1011 /f<-5.66
wherein f is L1011 Denotes a focal length of the third cemented lens, and f denotes a focal length of the objective lens.
Specifically, see FIG. 1,f L1011 Indicating the focal length of the third cemented lens formed by lenses L10 and L11.
Optionally, the focal length of the fourth cemented lens satisfies the following relation:
-69.1<f L1213 /f<-70.2
wherein f is L1213 Denotes a focal length of the fourth cemented lens, and f denotes a focal length of the objective lens.
Specifically, see FIG. 1,f L1213 Showing the formation of lenses L12 and L13Focal length of the quadruple cemented lens.
In a specific embodiment, the specific parameters of each lens in the objective lens are shown in table one. Referring to fig. 1, R1-R21 in fig. 1 correspond to the surface numbers 1-21 in table one, respectively.
Watch 1
Referring to fig. 2, fig. 2 is a Modulation Transfer Function (MTF) curve of the objective lens in the above embodiment. As can be seen from FIG. 2, the cut-off frequency of the objective lens is greater than 3100lp/mm, and the full field of view range approaches the diffraction limit; the objective lens has excellent image quality and good aberration correction.
Referring to fig. 3, fig. 3 is a dot diagram of the objective lens in the above embodiment, and it can be seen from fig. 3 that the full field of view speckle size is around the airy disk size range at visible wavelengths. Referring to fig. 4, fig. 4 (a) is a field curvature diagram of the objective lens in the above embodiment, and fig. 4 (b) is a distortion curve of the objective lens in the above embodiment, it can be seen from fig. 4 that the imaging field of view of the lens is flat, and the distortion is less than 0.85%. The result data of fig. 3 and 4 show that the objective lens achieves the flat field apochromatism effect.
Referring to fig. 5, fig. 5 is a diffraction energy distribution curve of the objective lens in the above embodiment, where the abscissa is the diameter of the scattered spot and the ordinate is the percentage of energy of the scattered spot. As can be seen from FIG. 5, 90% of the scattered spot energy is concentrated in the range of 1um, and the energy concentration is high, so that the objective lens is particularly suitable for weak fluorescence signal imaging in gene sequencing.
In the above embodiment, the numerical aperture of the objective lens is 0.75, the diameter of the field of view on the imaging object side is 1.6mm, the flat field apochromatism and the focal length are 10mm, which can satisfy most high-throughput sequencing applications.
Optionally, all lenses of the first, second and third lens groups are spherical lenses.
Specifically, all the lenses L2 to L13 in the first lens group to the third lens group are spherical lenses, thereby reducing the processing cost of the lenses.
The implementation of the embodiment of the invention has the following beneficial effects: the objective lens in the embodiment comprises three lens groups coaxially arranged from an object side to an image side, and the first lens group comprises two meniscus lenses; the second lens group includes two cemented lenses and a double convex lens having an intermediate refractive power, wherein the first cemented lens composed of two double convex lenses and a double concave lens and the second cemented lens composed of a meniscus lens and a double convex lens; the third lens group comprises two cemented lenses, wherein the third cemented lens is composed of two meniscus lenses, and the fourth cemented lens is composed of a biconcave lens and a biconvex lens; the light emitted by the object to be detected firstly passes through the first lens group to reduce the aperture angle, then the second lens group corrects one or more of spherical aberration, coma aberration or chromatic aberration, and then the third lens group eliminates one or more of field curvature, astigmatism or chromatic aberration, so that the imaging visual field and the numerical aperture are simultaneously increased, and the sequencing flux is improved.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the spirit and scope of the invention as defined by the appended claims.
Claims (8)
1. A high-flux flat field apochromatic objective lens is characterized by comprising a first lens group, a second lens group and a third lens group which are coaxially arranged in sequence from an object space to an image space, wherein,
the first lens group comprises a first meniscus lens and a second meniscus lens which are sequentially arranged;
the second lens group comprises a first biconvex lens, a first biconcave lens, a second biconvex lens, a third meniscus lens and a fourth biconvex lens which are sequentially arranged, wherein the first biconvex lens, the first biconcave lens and the second biconvex lens form a first cemented lens, and the third meniscus lens and the fourth biconvex lens form a second cemented lens;
the third lens group comprises a fourth meniscus lens, a fifth meniscus lens, a second biconcave lens and a fifth biconvex lens which are arranged in sequence, the fourth meniscus lens and the fifth meniscus lens form a third cemented lens, and the second biconcave lens and the fifth biconvex lens form a fourth cemented lens.
2. The objective lens according to claim 1, wherein a focal length of the first lens group satisfies the following relation:
10.2<f L23 /f<11
wherein f is L23 Denotes a focal length of the first lens group, and f denotes a focal length of the objective lens.
3. The objective lens according to claim 1, wherein the focal length of the first cemented lens satisfies the following relation:
6.42<f L456 /f<7.15
wherein f is L456 Denotes a focal length of the first cemented lens, and f denotes a focal length of the objective lens.
4. The objective lens according to claim 1, wherein the focal length of the third biconvex lens satisfies the following relation:
2.91<f L7 /f<3.32
wherein f is L7 Denotes a focal length of the third lenticular lens, and f denotes a focal length of the objective lens.
5. The objective lens according to claim 1, wherein the focal length of the second cemented lens satisfies the following relation:
10.6<f L89 /f<12.3
wherein f is L89 Denotes a focal length of the second cemented lens, and f denotes a focal length of the objective lens.
6. The objective lens according to claim 1, wherein the focal length of the third cemented lens satisfies the following relation:
-6.01<f L1011 /f<-5.66
wherein f is L1011 Denotes a focal length of the third cemented lens, and f denotes a focal length of the objective lens.
7. The objective lens according to claim 1, wherein the focal length of the fourth cemented lens satisfies the following relation:
-69.1<f L1213 /f<-70.2
wherein f is L1213 Denotes a focal length of the fourth cemented lens, and f denotes a focal length of the objective lens.
8. The objective lens of claim 1, wherein the first meniscus lens and the second meniscus lens are both spherical lenses.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211005330.0A CN115327745B (en) | 2022-08-22 | 2022-08-22 | High-flux flat-field apochromatic objective lens |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211005330.0A CN115327745B (en) | 2022-08-22 | 2022-08-22 | High-flux flat-field apochromatic objective lens |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115327745A true CN115327745A (en) | 2022-11-11 |
| CN115327745B CN115327745B (en) | 2024-01-16 |
Family
ID=83926038
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211005330.0A Active CN115327745B (en) | 2022-08-22 | 2022-08-22 | High-flux flat-field apochromatic objective lens |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115327745B (en) |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100202062A1 (en) * | 2007-11-02 | 2010-08-12 | Nikon Corporation | Immersion type microscope objective lens |
| CN108957718A (en) * | 2017-05-27 | 2018-12-07 | 南京理工大学 | A kind of wide spectrum flat field apochromatic micro objective |
| CN109061862A (en) * | 2018-08-27 | 2018-12-21 | 中国科学院苏州生物医学工程技术研究所 | A kind of micro objective of big field of view number |
| CN114326071A (en) * | 2021-12-14 | 2022-04-12 | 宁波永新光学股份有限公司 | 20-time large numerical aperture flat field apochromatic microscope objective |
| CN114815134A (en) * | 2021-01-20 | 2022-07-29 | 长春长光华大智造测序设备有限公司 | Achromatic microscope objective and optical system |
-
2022
- 2022-08-22 CN CN202211005330.0A patent/CN115327745B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20100202062A1 (en) * | 2007-11-02 | 2010-08-12 | Nikon Corporation | Immersion type microscope objective lens |
| CN108957718A (en) * | 2017-05-27 | 2018-12-07 | 南京理工大学 | A kind of wide spectrum flat field apochromatic micro objective |
| CN109061862A (en) * | 2018-08-27 | 2018-12-21 | 中国科学院苏州生物医学工程技术研究所 | A kind of micro objective of big field of view number |
| CN114815134A (en) * | 2021-01-20 | 2022-07-29 | 长春长光华大智造测序设备有限公司 | Achromatic microscope objective and optical system |
| CN114326071A (en) * | 2021-12-14 | 2022-04-12 | 宁波永新光学股份有限公司 | 20-time large numerical aperture flat field apochromatic microscope objective |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115327745B (en) | 2024-01-16 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP3299808B2 (en) | Immersion microscope objective lens | |
| CN108873289B (en) | Microscope objective optical system and optical device | |
| CN109061862B (en) | Microscope objective lens with large visual field number | |
| JP3559623B2 (en) | Imaging lens | |
| JPH05119263A (en) | Microscope objective | |
| CN116909007B (en) | Large-view-field flat-field apochromatic microscope objective lens | |
| JPH07294808A (en) | Image forming lens | |
| JPS59149312A (en) | Photographic lens of high aperture ratio | |
| JP2003015047A (en) | Immersion system microscope objective | |
| CN114019665A (en) | Microscope objective | |
| JP4959230B2 (en) | Microscope objective lens | |
| JP2002350734A (en) | Liquid immersion system microscopic objective lens | |
| JPH10301021A (en) | Small-sized lens | |
| US4063802A (en) | Telephotographic lens system having short total length | |
| JPH10268188A (en) | Large-aperture lens for photographic at low illuminance | |
| CN115327745B (en) | High-flux flat-field apochromatic objective lens | |
| JPH09127414A (en) | Lens for reading | |
| RU2308063C1 (en) | Fast high-speed lens | |
| JPH08136816A (en) | Objective lens of microscope | |
| US5532879A (en) | Microscope objective lens | |
| JPH06160721A (en) | High magnification microscope objective lens | |
| CN216351509U (en) | Microscope objective | |
| CN113589497B (en) | Ultraviolet lens for limited conjugate distance imaging | |
| JPS61275810A (en) | Microscope objective | |
| US11467372B2 (en) | Lens system and image pickup apparatus |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |