CN115316378A

CN115316378A - Tumor sample low temperature storage device

Info

Publication number: CN115316378A
Application number: CN202211263993.2A
Authority: CN
Inventors: 赖宇雄; 刘兆宇; 周新科
Original assignee: Fifth Affiliated Hospital Of Guangzhou Medical University Guangdong Provincial Laboratory Affiliated Hospital Of Guangzhou Regenerative Medicine And Health
Current assignee: Fifth Affiliated Hospital Of Guangzhou Medical University Guangdong Provincial Laboratory Affiliated Hospital Of Guangzhou Regenerative Medicine And Health
Priority date: 2022-10-17
Filing date: 2022-10-17
Publication date: 2022-11-11
Anticipated expiration: 2042-10-17
Also published as: CN115316378B

Abstract

The invention discloses tumor sample low-temperature storage equipment which comprises a tumor tissue storage table, a support frame, liquid supply cylinders, a tumor tissue storage mechanism and a monomer taking and placing type sealing mechanism, wherein the support frame is arranged on the bottom wall of the tumor tissue storage table, the liquid supply cylinders are symmetrically arranged at two ends of the tumor tissue storage table, the tumor tissue storage mechanism is arranged on the bottom wall of the tumor tissue storage table, the tumor tissue storage mechanism comprises a tissue bearing mechanism and an air flow discharging mechanism, and the tissue bearing mechanism is arranged on the bottom wall of the tumor tissue storage table. The invention belongs to the technical field of tissue sample storage, and particularly relates to a tumor sample low-temperature storage device; the invention provides a tumor sample low-temperature storage device which can inhibit the growth of ice crystals on the surface of tumor tissues and can effectively protect tumor tissue cells at low temperature.

Description

Tumor sample low temperature storage device

Technical Field

The invention belongs to the technical field of tissue sample storage, and particularly relates to tumor sample low-temperature storage equipment.

Background

After obtaining a new tumor tissue, the cell line in the tumor tissue is first cultured and expanded and then frozen. Tumor tissue cryopreservation can be used for backup when cells are lost due to pollution and other conditions, in addition, almost all tumor tissue cells are mutated to a certain extent in the process of culture and passage, and generally, the tumor tissue cells cannot be used after being cultured for dozens of generations, so that the tumor tissue needs to be cryopreserved, and the cells can be cultured again for use after the tumor tissue is recovered.

The existing liquid nitrogen storage device is mostly stored manually, the storage process is quite complicated, and as the temperature of the liquid nitrogen can reach-196 ℃ at the lowest, the hand is manually inserted into the liquid nitrogen space for operation, so that great threat is easily caused to the safety of operators; and when the tumor tissue is stored, ice crystals are easy to appear on the surface of the tumor tissue, so that cells in the tissue are damaged mechanically and lose activity.

Disclosure of Invention

Aiming at the situation and overcoming the defects of the prior art, the scheme provides a tumor sample low-temperature storage device, aiming at the problem that ice crystals are easy to appear on the surface of tumor tissues during low-temperature storage, the cryopreservation agent mixing injection structure is creatively combined with the sealing structure, and through the arranged liquid mixing damage-free mechanism and the sealing type communication storage mechanism, the tumor tissue cells are stored at low temperature without mechanical damage under the intervention of an inhibitor, so that the problem that the tumor tissues are scrapped due to the fact that the cells are easily mechanically damaged due to the generation of the ice crystals on the surface in the cryopreservation process of the tumor tissues, which is difficult to solve by the prior art, is solved; the problem that operators take and place the storage container manually is solved by the arrangement of the automatic lifting structure;

the invention provides a tumor sample low-temperature storage device which can inhibit the growth of ice crystals on the surface of tumor tissue and effectively protect tumor tissue cells at low temperature.

The technical scheme adopted by the scheme is as follows: the scheme provides a tumor sample low-temperature storage device, deposit platform, support frame, liquid supply section of thick bamboo, tumor tissue and deposit mechanism and monomer including tumor tissue and get the type of putting and seal up deposit mechanism, the support frame is located tumor tissue and is deposited a diapire, the tumor tissue is deposited a both ends to liquid supply section of thick bamboo symmetry, tumor tissue is located tumor tissue and is deposited a diapire by tumor tissue deposit mechanism, tumor tissue is deposited the mechanism and is born the mechanism and air current discharge mechanism including the tissue, the tissue bears the mechanism and locates tumor tissue and deposit a diapire, air current discharge mechanism locates the tissue and bears the mechanism bottom, tumor tissue deposit mechanism upper wall is located to monomer get the type of putting seal up deposit mechanism, monomer gets the type of putting and seals up deposit mechanism and includes seal up deposit mechanism and sealed mechanism, seal up deposit mechanism and locate the mechanism upper wall that seals up deposit.

According to a further preferable scheme of the scheme, the tissue bearing mechanism comprises a storage opening, a freezing storage box, a ring electromagnet, an iron plate, a vibration reduction spring, a storage barrel, a bearing screen plate and internal threads, wherein the storage opening is formed in the upper wall of the tumor tissue storage table, the freezing storage box is arranged in the inner wall of the storage opening, the freezing storage box is a cavity with an upper end opened, multiple groups of the ring electromagnets are arranged at the bottom of the freezing storage box, multiple groups of the iron plate are attached to the bottom wall of the freezing storage box above the ring electromagnet, the vibration reduction spring is arranged on the upper wall of the iron plate, the storage barrel is arranged on one side, away from the iron plate, of the vibration reduction spring, the storage barrel is arranged with an upper end opened, the internal threads are arranged on the inner wall of the opening of the storage barrel, and the bearing screen plate is arranged on the inner wall of the storage barrel below the internal threads; the air flow discharging mechanism comprises an ultrasonic atomizer, a flexible hose, an air fog pipe, a flow dividing pipe and a one-way exhaust valve, the ultrasonic atomizer is arranged on the bottom wall of the liquid supply barrel, the power end of the ultrasonic atomizer penetrates through the inner wall of the liquid supply barrel, the flexible hose sequentially penetrates through the annular electromagnet, the freezing box, the iron plate and the damping spring and is communicated with the bottom wall of the storage barrel, the air fog pipe is symmetrically arranged on the upper wall of the liquid supply barrel, the air fog pipe is communicated with the upper wall of the liquid supply barrel, the flow dividing pipe is communicated with the flexible hose and the air fog pipe, and the one-way exhaust valve is communicated with the side wall of the storage barrel; place the tumor tissue to bear the weight of otter board upper wall, ring electro-magnet circular telegram produces magnetism, the ring electro-magnet sees through freezing the bottom wall of deposit case and carries out magnetic force to adsorb to iron plate, iron plate carries out dynamic positioning through damping spring to the storage cylinder, at this moment, the inside air impurity that leaves of storage cylinder, ultrasonic atomizer carries out the oscillation atomization through the hemicellulose B solution anti-ice crystal inhibitor of power end inside the liquid supply section of thick bamboo, fog passes through the fog pipe and enters into the expansion tube through the shunt tubes inside, the expansion tube carries the fog inside the storage cylinder, the growth of suppression tumor tissue surface ice crystal.

Preferably, the sealing mechanism comprises a guide hole, a guide post, a lifting frame, a sealing electromagnet and a lifting electromagnet, wherein multiple groups of guide holes are formed in the upper wall of the freezing box, the guide post is slidably arranged on the inner wall of the guide hole, the lifting frame is arranged on the upper wall of the guide post, the sealing electromagnet is arranged on the bottom wall of the guide hole, the lifting electromagnet is arranged on the bottom wall of the guide post, and the sealing electromagnet and the lifting electromagnet are oppositely arranged; the sealing storage mechanism comprises a cover plate, a separation threaded hole, a rotating block, a rotating frame, a sealing cylinder, external threads, a taking and placing threaded hole, a taking and placing bolt, a locking nut, a sealing spring and a connecting block, wherein the cover plate is arranged on the inner wall of the lifting frame, a plurality of groups of the separation threaded holes are formed in the upper wall of the cover plate, the rotating block is arranged in the separation threaded hole and is in threaded connection with the separation threaded hole, the rotating frame is arranged on the upper wall of the rotating block, the taking and placing threaded hole is formed in the upper wall of the rotating block, the taking and placing bolt is arranged in the taking and placing threaded hole and is in threaded connection with the taking and placing threaded hole, the sealing spring is arranged on the bottom wall of the rotating block outside the taking and placing bolt, the connecting block is arranged on one side, away from the rotating block, of the sealing cylinder is rotatably arranged on one side, away from the sealing spring, the sealing cylinder is a cavity with an opening at the lower end, the taking and placing bolt penetrates through the connecting block and placing end, the outer side wall of the rotating block, the locking nut is arranged on the outer side wall of the sealing cylinder, and the taking and placing bolt is arranged on the opening; the sealing electromagnet and the lifting electromagnet are electrified to generate magnetism, the sealing electromagnet and the lifting electromagnet are arranged in the same pole, the sealing electromagnet pushes the lifting electromagnet to ascend through repulsion force, the lifting electromagnet drives the guide post to slide along the inner wall of the guide hole, the guide post drives the lifting frame to ascend, the lifting frame drives the cover plate to ascend and is far away from the upper wall of the freezing storage box, after the tumor tissue storage is completed, the repulsion force between the sealing electromagnet and the lifting electromagnet is reduced, the lifting electromagnet drives the guide post to slowly descend along the guide hole, after the sealing electromagnet is attached to the lifting electromagnet, the magnetic pole of the sealing electromagnet is changed, the heteropolar setting between the sealing electromagnet and the lifting electromagnet is realized, the sealing electromagnet and the lifting electromagnet are adsorbed through magnetic force, the lifting frame is attached to the upper wall of the freezing storage box, the cover plate seals the freezing storage box, the rotating block is locked inside the separation threaded hole through the rotating frame, the locking nut is rotated to be far away from the upper wall of the rotating block along the taking and placing bolt, the bolt is in a loose state, the taking and placing bolt is rotated to be close to the taking and the placing of the placing and placing storage cylinder, and the external thread is rotated to be close to the fixing bolt.

Specifically, the tumor tissue storage table is provided with a control button on the side wall.

Wherein, control button respectively with ring electromagnet, ultrasonic nebulizer, seal up deposit electro-magnet and lift electro-magnet electric connection.

The beneficial effect who adopts above-mentioned structure this scheme to gain as follows:

(1) According to the scheme, the stored tumor tissues can be taken out in a single body through the arranged split mechanism, so that the other stored tumor tissues are prevented from being taken out repeatedly, the rotating block is rotated through the rotating frame and is rotated out along the separation threaded hole, the rotating block drives the sealing cylinder to ascend through the taking and placing bolt, and the sealing cylinder drives the storage cylinder to be far away from the interior of the freezing storage box, so that the single tumor tissues are taken out;

(2) Redundant air in the storage barrel is discharged through the arranged one-way exhaust valve, so that the activity storage efficiency of tumor tissues is improved, an inhibitor in the liquid supply barrel is atomized through the arranged ultrasonic atomizer, fog enters the telescopic hose through the fog pipe through the flow dividing pipe, the telescopic hose injects the fog into the storage barrel, and the air in the storage barrel is extruded out through the one-way exhaust valve by the gushed fog;

(3) Through the dynamic fixing mechanism who sets up, can accomplish under the effect of ring electro-magnet and take out with the separation the developments of storage cylinder, simultaneously under damping spring's elastic deformation, when needs are whole take out to freezing incasement portion tumour tissue, damping spring passes through the elasticity extension and drives the storage cylinder and float out from the liquid nitrogen to avoid the operation personnel to deepen the liquid nitrogen and take out the storage cylinder, reduce operation personnel injured probability.

Drawings

Fig. 1 is a schematic diagram of an overall structure of a tumor sample cryogenic storage apparatus according to the present embodiment;

fig. 2 is a perspective view of a tumor sample cryogenic storage device according to the present disclosure;

fig. 3 is an oblique view of a tumor sample cryogenic storage device according to the present solution;

fig. 4 is an exploded view of a tumor sample cryogenic storage device according to the present disclosure;

fig. 5 is a top view of a tumor sample cryogenic storage device according to the present scheme;

FIG. 6 isbase:Sub>A partial sectional view A-A of FIG. 5;

FIG. 7 is a partial sectional view taken along line B-B of FIG. 5;

FIG. 8 is an enlarged view of the portion A of FIG. 2;

FIG. 9 is an enlarged view of the portion B of FIG. 3;

fig. 10 is a schematic structural diagram of a freezing storage box of a tumor sample low-temperature storage device according to the present scheme;

fig. 11 is a schematic structural diagram of a lifting frame of a tumor sample low-temperature storage device according to the present embodiment;

fig. 12 is a schematic structural diagram of a tumor tissue storage table of a tumor sample cryogenic storage device according to the present disclosure.

The tumor tissue storage device comprises a tumor tissue storage table 1, a support frame 2, a liquid supply cylinder 3, a liquid supply cylinder 4, a tumor tissue storage mechanism 5, a tissue bearing mechanism 6, a storage opening 7, a freezing storage box 8, a ring electromagnet 9, an iron plate 10, a vibration damping spring 11, a storage cylinder 12, a bearing screen plate 13, an internal thread 14, an airflow discharge mechanism 15, an ultrasonic atomizer 16, a flexible hose 17, a mist pipe 18, a shunt pipe 19, a one-way exhaust valve 20, a single taking and placing type sealing mechanism 21, a sealing mechanism 22, a guide hole 23, a guide column 24, a lifting frame 25, a sealing electromagnet 26, a lifting electromagnet 27, a sealing storage mechanism 28, a cover plate 29, a separation threaded hole 30, a rotating block 31, a rotating frame 32, a sealing cylinder 33, an external thread 34, a threaded hole 35, a taking and placing bolt 36, a locking nut 37, a sealing spring, 38, a connecting block 39 and a control button.

The accompanying drawings are included to provide a further understanding of the present solution and are incorporated in and constitute a part of this specification, illustrate embodiments of the solution and together with the description serve to explain the principles of the solution and not to limit the solution.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments; all other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present disclosure without any creative effort belong to the protection scope of the present disclosure.

In the description of the present solution, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present solution.

As shown in fig. 1-3, the present embodiment provides a tumor sample low-temperature storage device, which includes a tumor tissue storage platform 1, a support frame 2, a liquid supply cylinder 3, a tumor tissue storage mechanism 4, and a single body taking and placing type sealing mechanism 20, wherein the support frame 2 is disposed on the bottom wall of the tumor tissue storage platform 1, the liquid supply cylinder 3 is symmetrically disposed at two ends of the tumor tissue storage platform 1, the tumor tissue storage mechanism 4 is disposed on the bottom wall of the tumor tissue storage platform 1, the tumor tissue storage mechanism 4 includes a tissue bearing mechanism 5 and an airflow discharging mechanism 14, the tissue bearing mechanism 5 is disposed on the bottom wall of the tumor tissue storage platform 1, the airflow discharging mechanism 14 is disposed at the bottom of the tissue bearing mechanism 5, the single body taking and placing type sealing mechanism 20 is disposed on the upper wall of the tumor tissue storage mechanism 4, the single body taking and placing type sealing mechanism 20 includes a sealing mechanism 21 and a sealing storage mechanism 27, the sealing mechanism 21 is disposed on the upper wall of the tissue bearing mechanism 5, and the sealing storage mechanism 27 is disposed on the upper wall of the sealing mechanism 21.

As shown in fig. 1-4, 7, 8, 10 and 12, the tissue bearing mechanism 5 includes a storage port 6, a freezing storage box 7, a ring electromagnet 8, an iron plate 9, a damping spring 10, storage cylinders 11, a bearing screen 12 and internal threads 13, the storage port 6 is disposed on the upper wall of the tumor tissue storage platform 1, the freezing storage box 7 is disposed on the inner wall of the storage port 6, the freezing storage box 7 is a cavity with an open upper end, multiple groups of the ring electromagnet 8 are disposed at the bottom of the freezing storage box 7, multiple groups of the iron plate 9 are attached to the bottom wall of the freezing storage box 7 above the ring electromagnet 8, the damping spring 10 is disposed on the upper wall of the iron plate 9, the storage cylinders 11 are disposed on the side of the damping spring 10 away from the iron plate 9, the storage cylinders 11 are disposed with open upper ends, the internal threads 13 are disposed on the inner wall of the openings of the storage cylinders 11, and the bearing screen 12 is disposed on the inner wall of the storage cylinders 11 below the internal threads 13; the air flow discharge mechanism 14 comprises an ultrasonic atomizer 15, a flexible hose 16, a fog pipe 17, a flow dividing pipe 18 and a one-way exhaust valve 19, wherein the ultrasonic atomizer 15 is arranged on the bottom wall of the liquid supply barrel 3, the power end of the ultrasonic atomizer 15 penetrates through the inner wall of the liquid supply barrel 3, the flexible hose 16 sequentially penetrates through a circular electromagnet 8, a freezing box 7, an iron plate 9 and a damping spring 10 and is communicated with the bottom wall of the storage barrel 11, the fog pipe 17 is symmetrically arranged on the upper wall of the liquid supply barrel 3, the fog pipe 17 is communicated with the upper wall of the liquid supply barrel 3, the flow dividing pipe 18 is communicated between the flexible hose 16 and the fog pipe 17, and the one-way exhaust valve 19 is communicated with the side wall of the storage barrel 11; place the tumor tissue and bear the weight of otter board 12 upper wall, 8 circular ring electro-magnets are electrified and are produced magnetism, circular ring electro-magnets 8 are passed through to freeze and are deposited 7 diapalls of case and carry out magnetic force absorption to iron plate 9, iron plate 9 carries out dynamic positioning through damping spring 10 to storage tube 11, at this moment, the inside air impurity that leaves of storage tube 11, ultrasonic nebulizer 15 passes through power end and prevents that the ice crystal inhibitor is carried out the vibration atomizing to the hemicellulose B solution of liquid supply section of thick bamboo 3 inside, fog passes through fog pipe 17 and enters into inside flexible hose 16 through shunt tubes 18, flexible hose 16 carries storage tube 11 with fog inside, the growth of tumor tissue surface ice crystal is suppressed.

As shown in fig. 1, fig. 2, fig. 4-fig. 7, fig. 9 and fig. 11, the sealing mechanism 21 includes a guiding hole 22, a guiding post 23, a lifting frame 24, a sealing electromagnet 25 and a lifting electromagnet 26, wherein multiple groups of the guiding hole 22 are disposed on the upper wall of the freezing storage box 7, the guiding post 23 is slidably disposed on the inner wall of the guiding hole 22, the lifting frame 24 is disposed on the upper wall of the guiding post 23, the sealing electromagnet 25 is disposed on the bottom wall of the guiding hole 22, the lifting electromagnet 26 is disposed on the bottom wall of the guiding post 23, and the sealing electromagnet 25 and the lifting electromagnet 26 are disposed opposite to each other; the sealing storage mechanism 27 comprises a cover plate 28, a separation threaded hole 29, a rotating block 30, a rotating frame 31, a sealing cylinder 32, external threads 33, a taking and placing threaded hole 34, a taking and placing bolt 35, a locking nut 36, a sealing spring 37 and a connecting block 38, wherein the cover plate 28 is arranged on the inner wall of the lifting frame 24, multiple groups of the separation threaded holes 29 are arranged on the upper wall of the cover plate 28, the rotating block 30 is arranged inside the separation threaded hole 29, the rotating block 30 is in threaded connection with the separation threaded hole 29, the rotating frame 31 is arranged on the upper wall of the rotating block 30, the taking and placing threaded hole 34 is arranged on the upper wall of the rotating block 30, the taking and placing bolt 35 is arranged inside the taking and placing threaded hole 34, the taking and placing bolt 35 is in threaded connection with the taking and placing threaded hole 34, the sealing spring 37 is arranged on the bottom wall of the rotating block 30 outside the taking and placing bolt 35, the connecting block 38 is arranged on one side, far away from the rotating block 30, the sealing cylinder 32 is rotatably arranged on one side, far away from the sealing spring 37, the sealing cylinder 32 is a cavity with an opening at the lower end, one far away from the rotating block 30, the outer side wall of the sealing cylinder 35 is arranged on the outer side wall of the sealing cylinder 35, and the external threads 33, and the sealing cylinder 35 is connected with the external threads 33; the sealing electromagnet 25 and the lifting electromagnet 26 are electrified to generate magnetism, the sealing electromagnet 25 and the lifting electromagnet 26 are arranged in the same pole, the sealing electromagnet 25 pushes the lifting electromagnet 26 to rise through repulsion force, the lifting electromagnet 26 drives the guide post 23 to slide along the inner wall of the guide hole 22, the guide post 23 drives the lifting frame 24 to rise, the lifting frame 24 drives the cover plate 28 to rise away from the upper wall of the freezing storage box 7, after the tumor tissues are stored, the repulsion force between the sealing electromagnet 25 and the lifting electromagnet 26 is reduced, the lifting electromagnet 26 drives the guide post 23 to slowly descend along the guide hole 22, after the sealing electromagnet 25 is attached to the lifting electromagnet 26, the magnetic pole of the sealing electromagnet 25 is changed, and the sealing electromagnet 25 and the lifting electromagnet 26 are arranged in a different pole, the sealing electromagnet 25 and the lifting electromagnet 26 are adsorbed by magnetic force, the lifting frame 24 is attached to the upper wall of the freezing storage box 7, the cover plate 28 seals the freezing storage box 7, the rotating block 30 is rotated through the rotating frame 31, the rotating block 30 is locked inside the separation threaded hole 29, the locking nut 36 is rotated, the locking nut 36 rotates along the taking and placing bolt 35 to be far away from the upper wall of the rotating block 30, the taking and placing bolt 35 is in a loosening state, the taking and placing bolt 35 is rotated, the taking and placing bolt 35 drives the sealing cylinder 32 to rotate to be close to the storage cylinder 11, the sealing cylinder 32 is screwed into the internal thread 13 through the external thread 33, the storage cylinder 11 is sealed through the sealing cylinder 32, the locking nut 36 is rotated, the locking nut 36 rotates along the bolt 35 to be close to the upper wall of the rotating block 30, and the taking and placing bolt 35 is fixed.

As shown in fig. 1, the tumor tissue storage table 1 is provided with a control button 39 on the side wall.

The control button 39 is electrically connected with the ring electromagnet 8, the ultrasonic atomizer 15, the sealing electromagnet 25 and the lifting electromagnet 26 respectively.

When the freezing storage box is used specifically, the bottom wall of the lifting frame 24 is attached to the upper wall of the freezing storage box 7 in an initial state, and a hemicellulose B solution anti-icing crystal inhibitor is injected into the liquid supply cylinder 3.

In the first embodiment, when tumor tissue needs to be stored in the cryopreservation box 7, the control button 39 controls the sealing electromagnet 25 and the lifting electromagnet 26 to be started, the sealing electromagnet 25 and the lifting electromagnet 26 are electrified to generate magnetism, the sealing electromagnet 25 and the lifting electromagnet 26 are arranged in the same polarity, the sealing electromagnet 25 pushes the lifting electromagnet 26 to rise through repulsive force, the lifting electromagnet 26 drives the guide post 23 to slide along the inner wall of the guide hole 22, the guide post 23 drives the lifting frame 24 to rise, the lifting frame 24 drives the cover plate 28 to rise and be away from the upper wall of the cryopreservation box 7, the control button 39 controls the circular electromagnet 8 to be started, the circular electromagnet 8 is electrified to generate magnetism, the circular electromagnet 8 carries out magnetic adsorption on the iron plate 9 through the bottom wall of the cryopreservation box 7, the iron plate 9 carries out dynamic positioning on the storage barrel 11 through the damping spring 10, the tumor tissue is placed on the upper wall of the bearing screen plate 12, at the moment, the damping spring 10 is in an extended state, liquid nitrogen is injected into the cryopreservation box 7, and the liquid nitrogen is injected into the bottom wall of the storage barrel 11;

the control button 39 controls the current passing through the sealing electromagnet 25 and the lifting electromagnet 26 to be reduced, the repulsive force between the sealing electromagnet 25 and the lifting electromagnet 26 is reduced, the lifting electromagnet 26 drives the guide post 23 to slowly descend along the guide hole 22, after the sealing electromagnet 25 is attached to the lifting electromagnet 26, the control button 39 controls the magnetic pole of the sealing electromagnet 25 to change, the heteropolar arrangement between the sealing electromagnet 25 and the lifting electromagnet 26 is realized, the sealing electromagnet 25 and the lifting electromagnet 26 are adsorbed by magnetic force, the lifting frame 24 is attached to the upper wall of the freezing box 7, the cover plate 28 seals the freezing box 7, the rotating block 30 is rotated through the rotating frame 31, the rotating block 30 is locked inside the separation threaded hole 29, the locking nut 36 is rotated, the locking nut 36 rotates along the taking and placing bolt 35 to be far away from the upper wall of the rotating block 30, the taking and placing bolt 35 is in a loose state, the rotating bolt 35 rotates, the taking and placing bolt 35 drives the sealing cylinder 32 to rotate, the sealing cylinder 32 to be close to the storage cylinder 11 through the deformation of the sealing spring 37, the sealing cylinder 32 is screwed into the internal thread 13 through the external thread 33, the sealing cylinder 32 seals the storage cylinder 11, the sealing cylinder 11, the damping spring 10 is elastically deformed to shorten the taking and the taking of the locking nut 36 to rotate the locking nut 35 to be close to the internal of the locking nut 35.

In the second embodiment, based on the above embodiment, after the tumor tissue is stored in the storage barrel 11, the external air also enters the storage barrel 11, so that air impurities are stored in the storage barrel 11, the control button 39 controls the ultrasonic atomizer 15 to start, the ultrasonic atomizer 15 vibrates and atomizes the hemicellulose B solution ice crystal inhibitor in the liquid supply barrel 3 through the power end, the mist enters the flexible hose 16 through the mist pipe 17 via the shunt pipe 18, and the flexible hose 16 conveys the mist into the storage barrel 11, so that on one hand, the growth of ice crystals on the surface of the tumor tissue is inhibited, the mechanical damage of tumor tissue cells is reduced, on the other hand, the gushing mist extrudes the air in the storage barrel 11, and the air is discharged out of the storage barrel 11 through the one-way exhaust valve 19, so as to better store the tumor tissue at a low temperature;

when a single tumor tissue needs to be taken out, the control button 39 controls the circular electromagnet 8 to be powered off, the power-off magnetism of the circular electromagnet 8 disappears, the rotating block 30 is screwed out of the separation threaded hole 29 through the rotating frame 31, the telescopic hose 16 extends, the storage barrel 11 cannot be taken out of the freezing storage box 7 through the separation threaded hole 29, and therefore the single tumor tissue is taken out; repeating the above operation when using next time.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present solution have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the solution, the scope of which is defined in the appended claims and their equivalents.

The present solution and its embodiments have been described above, but the description is not limited thereto, and what is shown in the drawings is only one of the embodiments of the present solution, and the actual structure is not limited thereto. In summary, those skilled in the art should appreciate that they can readily use the present disclosure without inventive faculty to devise similar arrangements and embodiments without departing from the spirit and scope of the present disclosure.

Claims

1. The utility model provides a tumour sample cryogenic storage equipment, includes that tumour tissue deposits platform (1), support frame (2) and supplies liquid cylinder (3), its characterized in that: still deposit mechanism (4) and monomer including tumour tissue and get the type and deposit mechanism (20) of putting, tumour tissue is located in support frame (2) and is deposited platform (1) diapire, supply liquid section of thick bamboo (3) symmetry to locate tumour tissue and deposit platform (1) both ends, tumour tissue is deposited mechanism (4) and is located tumour tissue and deposit platform (1) diapire, tumour tissue is deposited mechanism (4) and is born mechanism (5) and air current discharge mechanism (14) including the tissue, tumour tissue is deposited platform (1) diapire is located in tissue bearing mechanism (5), air current discharge mechanism (14) are located tissue and are born mechanism (5) bottom, tumour tissue is deposited mechanism (4) upper wall is located in monomer getting the type and deposit mechanism (20), mechanism (27) is deposited including sealing mechanism (21) and sealed in monomer getting the type and deposit mechanism (20), sealing mechanism (21) are located tissue and are born mechanism (5) upper wall, sealing mechanism (27) are deposited and are located and are sealed on mechanism (21) upper wall.

2. The tumor sample cryogenic storage device of claim 1, wherein: organize and bear mechanism (5) including depositing mouth (6), freezing case (7), ring electro-magnet (8), iron plate (9), damping spring (10), storage cylinder (11), bear otter board (12) and internal thread (13), deposit mouth (6) and locate tumor tissue and deposit platform (1) upper wall, freeze case (7) and locate and deposit mouth (6) inner wall, it is upper end open-ended cavity to freeze case (7) of depositing.

3. The tumor specimen cryogenic storage device of claim 2, wherein: ring electro-magnet (8) multiunit is located and is frozen case (7) bottom, freezing case (7) diapire of depositing above ring electro-magnet (8) is located in the laminating of iron plate (9) multiunit, iron plate (9) upper wall is located in damping spring (10), storage cylinder (11) are located damping spring (10) and are kept away from one side of iron plate (9), storage cylinder (11) are the upper end opening setting, storage cylinder (11) opening part inner wall is located in internal thread (13), storage cylinder (11) inner wall of internal thread (13) below is located in bearing otter board (12).

4. A tumor sample cryogenic storage device according to claim 3, wherein: airflow discharge mechanism (14) include ultrasonic nebulizer (15), expansion hose (16), fog pipe (17), shunt tubes (18) and one-way discharge valve (19), liquid supply section of thick bamboo (3) diapire is located in ultrasonic nebulizer (15), ultrasonic nebulizer (15) power end runs through and locates liquid supply section of thick bamboo (3) inner wall.

5. The tumor sample cryogenic storage device of claim 4, wherein: expansion hose (16) run through ring electro-magnet (8), freeze case (7), iron plate (9), damping spring (10) intercommunication in proper order and locate storage cylinder (11) diapire, supply liquid section of thick bamboo (3) upper wall is located to fog pipe (17) symmetry, supply liquid section of thick bamboo (3) upper wall is located in fog pipe (17) intercommunication, between expansion hose (16) and fog pipe (17) are located in shunt tubes (18) intercommunication, storage cylinder (11) lateral wall is located in one-way exhaust valve (19) intercommunication.

6. The tumor sample cryogenic storage device of claim 5, wherein: the sealing mechanism (21) comprises guide holes (22), guide columns (23), a lifting frame (24), sealing electromagnets (25) and lifting electromagnets (26), wherein the upper wall of the freezing storage box (7) is arranged in multiple groups of the guide holes (22), and the inner wall of the guide holes (22) is arranged in the guide columns (23) in a sliding manner.

7. The tumor sample cryogenic storage device of claim 6, wherein: the lifting frame (24) is arranged on the upper wall of the guide column (23), the sealing electromagnet (25) is arranged on the bottom wall of the guide hole (22), the lifting electromagnet (26) is arranged on the bottom wall of the guide column (23), and the sealing electromagnet (25) and the lifting electromagnet (26) are arranged oppositely.

8. The tumor sample cryogenic storage device of claim 7, wherein: sealed mechanism (27) of depositing is including apron (28), separation screw hole (29), commentaries on classics piece (30), rotating turret (31), sealed section of thick bamboo (32), external screw thread (33), get and put screw hole (34), get and put bolt (35), lock nut (36), seal up and deposit spring (37) and connecting block (38), lift frame (24) inner wall is located in apron (28), apron (28) upper wall is located to separation screw hole (29) multiunit, it is inside to change piece (30) to locate separation screw hole (29), changes piece (30) and separation screw hole (29) threaded connection, rotating turret (31) are located and are changeed piece (30) upper wall.

9. The tumor sample cryogenic storage device of claim 8, wherein: get and put screw hole (34) and locate commentaries on classics piece (30) upper wall, get and put bolt (35) and locate to get and put inside screw hole (34), get and put bolt (35) and get and put screw hole (34) threaded connection, seal up and deposit spring (37) and locate commentaries on classics piece (30) diapire of getting and putting bolt (35) outside, one side of keeping away from commentaries on classics piece (30) is located to seal up and deposit spring (37) connecting block (38), one side of keeping away from seal up and deposit spring (37) is located in sealing barrel (32) rotation, and sealing barrel (32) are lower extreme open-ended cavity.

10. The tumor sample cryogenic storage device of claim 9, wherein: get one end through connection piece (38) that the bolt (35) of putting kept away from commentaries on classics piece (30) and locate sealing cylinder (32) upper wall, lock nut (36) are located getting of commentaries on classics piece (30) top and are put bolt (35) outside, lock nut (36) with get and put bolt (35) threaded connection, sealing cylinder (32) opening part lateral wall is located in external screw thread (33).