CN115307581A - Plant leaf area measuring system based on photosynthetic apparatus and use method - Google Patents
Plant leaf area measuring system based on photosynthetic apparatus and use method Download PDFInfo
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- 241000196324 Embryophyta Species 0.000 claims abstract description 76
- 238000009529 body temperature measurement Methods 0.000 claims abstract description 66
- 238000001931 thermography Methods 0.000 claims abstract description 66
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- 238000012545 processing Methods 0.000 claims abstract description 10
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- 230000029553 photosynthesis Effects 0.000 claims description 10
- 238000010672 photosynthesis Methods 0.000 claims description 10
- 229910001148 Al-Li alloy Inorganic materials 0.000 claims description 4
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- 235000002568 Capsicum frutescens Nutrition 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01B—MEASURING LENGTH, THICKNESS OR SIMILAR LINEAR DIMENSIONS; MEASURING ANGLES; MEASURING AREAS; MEASURING IRREGULARITIES OF SURFACES OR CONTOURS
- G01B11/00—Measuring arrangements characterised by the use of optical techniques
- G01B11/28—Measuring arrangements characterised by the use of optical techniques for measuring areas
- G01B11/285—Measuring arrangements characterised by the use of optical techniques for measuring areas using photoelectric detection means
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Abstract
The invention discloses a plant leaf area measuring system based on a photosynthetic apparatus and a using method thereof, wherein the plant leaf area measuring system based on the photosynthetic apparatus comprises: the infrared thermal imaging temperature measurement camera system is arranged below an original leaf chamber on the photosynthetic instrument and is used for acquiring image information of plant leaves to be measured in real time; the auxiliary leaf chamber is arranged between the bottom of the original leaf chamber on the photosynthetic instrument and the infrared thermal imaging temperature measurement camera system, one end of the top of the auxiliary leaf chamber is hermetically communicated with the bottom of the original leaf chamber on the photosynthetic instrument, and one end of the bottom of the auxiliary leaf chamber is hermetically covered on the infrared thermal imaging temperature measurement camera system; and the picture information processing module is used for processing the image information of the plant leaves to be measured to obtain the leaf area information and the leaf temperature information of the plant leaves to be measured. According to the invention, the leaf chamber is improved, and the infrared thermal imaging temperature measurement camera system is additionally arranged, so that the real-time measurement of the leaf area and the leaf temperature is realized, the photosynthetic apparatus can measure smaller leaves, and the measurement accuracy of the photosynthetic apparatus is greatly improved.
Description
Technical Field
The invention relates to the technical field of plant leaf area measurement, in particular to a plant leaf area measurement system based on a photosynthetic apparatus and a use method thereof.
Background
The LI-COR6400/6800 portable photosynthetic determination system (LI-6400/6800) is a main instrument for the research of plant photosynthetic physiological ecology at present, is widely applied to the teaching and scientific research of related subjects such as forestry, agriculture, ecology, plant protection and the like, and is a large-scale basic instrument for the research and teaching of the related colleges and universities and research institutions of the agriculture, forestry and life science in China. LI-6400 according to leaf area and leaf temperature、CO 2 And H 2 And (4) calculating photosynthetic and moisture physiological related indexes such as net photosynthetic rate (Pn), intercellular carbon dioxide concentration (Ci), transpiration rate (Tr), stomatal conductance (Gs) and the like according to the change condition of O, and drawing a photosynthetic daily change curve, a photoresponse curve and a CO2 response curve. The leaf area is basic data of related parameters of photosynthesis and water utilization calculated by the photosynthesis meter, the accuracy of the leaf area influences the accuracy of photosynthetic indexes, and the leaf area is very important for subsequent photosynthetic physiological research and analysis of a photoresponse curve and a carbon dioxide response curve and is a key parameter of a photosynthetic measurement system.
Based on years of teaching and scientific research practice, most of the plants to be detected in LI-6400/6800 are plants with larger leaf areas or can be matched with the plant types with leaf chambers of the instrument. At present, because LI-6400/6800 lacks of a component for directly measuring the leaf area, for the leaf which is smaller than the area of the standard leaf chamber of LI-6400/6800, such as the strip-shaped leaves, lobular plants and irregular shapes (such as tippet leaves, broad oval leaves, needle leaves, pinnate hemiphragma leaves, heart-shaped leaves and halberd leaves), like wheat, hot pepper, jujube tree juveniles and acacia, because LI-6400/6800 has no module for measuring the leaf area, the accurate area of the leaf chamber is difficult to calculate, so that the leaf area must be measured by destructive sampling, and the experimental continuity is influenced. Destructive sampling methods are not suitable for limited sampling or long-term testing to track the growth, leaf expansion or stress tolerance of the same plant. In addition, blade area measurement errors may result due to certain changes in the angle and orientation of the blades during sampling. LI-6400/6800 is used for carrying out continuous tests such as stress, destructive sampling and measuring of leaf area greatly influences experimental results, and in addition, for the leaf chambers with different sizes, the convenience and the economy of the instrument are reduced due to the fact that the adaptive leaf chambers need to be replaced. However, adding a blade area measurement module faces two technical challenges:
1) The top of the leaf chamber is a light source, and a leaf area measuring module cannot be installed;
2) The bottom of the leaf chamber is provided with a temperature measuring probe (a leaf temperature thermocouple), a leaf area measuring module is not installed in space, and a common camera is difficult to shoot the leaves with clear edges due to interference of a top light source, so that the leaf area cannot be accurately measured. Therefore, the matched blade area measuring module needs to be installed at the bottom of the blade chamber, has the temperature measuring function and can clearly shoot the blade profile under the interference of background light.
In addition, if a leaf area measuring instrument such as a leaf area analyzer is purchased, the economy, portability and applicability of the LI-6400/6800 are seriously affected due to the high price of the leaf area measuring instrument. In addition, the specific orientation of the leaf blade in the leaf chamber has certain change in the moving process, so that the sampling and measuring leaf area has certain error, and the actual error in the calculation of the photosynthetic parameter is larger (> 20%). Therefore, there is a need to develop a lossless and accurate LI-6400/6800 leaf area measurement module.
Disclosure of Invention
The invention aims to provide a plant leaf area measuring system based on a photosynthetic apparatus, which can greatly improve the data credibility, applicability and convenience of LI-6400/6800 by modifying a leaf chamber, adding an infrared thermal imaging temperature measurement camera system and matching with an original leaf chamber of the LI-6400/6800 photosynthetic apparatus to perform nondestructive measurement on the leaf area in the leaf chamber.
The invention provides a plant leaf area measuring system based on a photosynthetic apparatus, which is arranged on the photosynthetic apparatus and comprises:
the infrared thermal imaging temperature measurement camera system is arranged below a primary leaf chamber on the photosynthetic instrument and is used for acquiring image information of plant leaves to be measured in real time;
the auxiliary leaf chamber is arranged between the bottom of the original leaf chamber on the photosynthetic apparatus and the infrared thermal imaging temperature measurement camera system, one end of the top of the auxiliary leaf chamber is hermetically communicated with the bottom of the original leaf chamber on the photosynthetic apparatus, and one end of the bottom of the auxiliary leaf chamber is hermetically covered on the infrared thermal imaging temperature measurement camera system;
and the picture information processing module is used for processing the image information of the plant leaves to be measured, which is acquired by the infrared thermal imaging temperature measurement camera system, so as to obtain the leaf area information and the leaf temperature information of the plant leaves to be measured.
Furthermore, the auxiliary leaf chamber is a rectangular cylinder, and the cross section of the auxiliary leaf chamber is a rectangular surface with the length of 3cm multiplied by 3 cm.
Furthermore, the top of the auxiliary leaf chamber is provided with an opening, and the opening at the top is sleeved at the bottom of the original leaf chamber on the photosynthetic instrument and is communicated with the bottom of the original leaf chamber on the photosynthetic instrument in a sealing way;
the bottom surface of the auxiliary blade chamber is provided with an installation groove;
the camera lens of the infrared thermal imaging temperature measurement camera system is located in the mounting groove and faces the top opening of the auxiliary leaf chamber, and the outer wall of the camera lens of the infrared thermal imaging temperature measurement camera system is connected with the mounting groove in a sealing mode.
Further, the distance between a camera lens of the infrared thermal imaging temperature measurement camera system and the top opening of the auxiliary leaf chamber is larger than 20cm.
Further, the auxiliary vane chamber is made of an aluminum lithium alloy.
Further, the infrared thermal imaging temperature measurement camera system sends the image information of the plant leaves to be measured, which is shot in real time, to the picture information processing module, and the picture information processing module processes and analyzes the image information to obtain the leaf area information and the leaf temperature information of the plant leaves to be measured.
The invention provides a method for using a plant leaf area measuring system based on a photosynthetic apparatus, which comprises the following steps:
arranging an infrared thermal imaging temperature measurement camera system below an original leaf chamber on the photosynthetic instrument;
arranging an auxiliary leaf chamber between the bottom of a primary leaf chamber on the photosynthetic instrument and the infrared thermal imaging temperature measurement camera system, and hermetically communicating one end of the top of the auxiliary leaf chamber with the bottom of the primary leaf chamber on the photosynthetic instrument, and hermetically covering one end of the bottom on the infrared thermal imaging temperature measurement camera system;
placing plant leaves to be measured in an auxiliary leaf chamber, and acquiring image information of the plant leaves to be measured by using an infrared thermal imaging temperature measurement camera system;
the infrared thermal imaging temperature measurement camera system transmits the acquired image information to the picture information processing module, and the picture information processing module processes the acquired image information to obtain leaf area information and leaf temperature information of the plant leaves to be measured.
Compared with the prior art, the invention has the following beneficial effects:
in the invention, the auxiliary leaf chamber is additionally arranged at the lower part of the original leaf chamber of the LI-6400/6800 photosynthetic apparatus, and the infrared thermal imaging temperature measurement camera system for acquiring the leaf image information of the plant to be measured is arranged in the auxiliary leaf chamber, so that the technical defect that the LI-6400/6800 photosynthetic apparatus cannot measure the leaf area is creatively solved, in addition, because the infrared thermal imaging temperature measurement camera system is an infrared thermal imaging temperature measurement camera system, the temperature measurement on the leaf area of the plant is realized, and the nondestructive measurement of the leaf area is realized, and the problem that the accurate area of the leaf in the leaf chamber is difficult to calculate because no module for measuring the leaf area is arranged in the conventional LI-6400/6800 photosynthetic apparatus, and the continuity of the experiment is influenced because the leaf area is measured by destructive sampling is solved. In addition, the real-time measurement of the leaf area and the leaf temperature is realized by modifying the leaf chamber and additionally installing the infrared thermal imaging temperature measurement camera system, the measurement accuracy of the photosynthetic instrument is greatly improved, the LI-6400/6800 photosynthetic instrument can measure smaller leaves, and the applicability of the LI-6400/6800 photosynthetic instrument is enhanced.
Drawings
The accompanying drawings, which are included to provide a further understanding of the invention and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the principles of the invention and not to limit the invention. In the drawings:
FIG. 1 is a schematic structural diagram of a plant leaf area measuring system based on a photosynthetic apparatus according to the present invention;
FIG. 2 is a schematic diagram of a system structure of a plant leaf area measuring system based on a photosynthetic apparatus according to the present invention;
FIG. 3 is a schematic flow chart illustrating the operation principle of a system for measuring the leaf area of a plant based on a photosynthetic apparatus according to the present invention;
fig. 4 is a schematic diagram illustrating the effect comparison between the infrared blade image (right) taken by the infrared thermal imaging temperature measurement camera system and the camera image (left) taken by the infrared thermal imaging temperature measurement camera system in the embodiment of the plant leaf area measurement system based on the photosynthetic apparatus according to the present invention;
fig. 5 is a schematic diagram of temperature measurement in an embodiment of a plant leaf area measurement system based on a photosynthetic apparatus according to the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention are clearly and completely described below with reference to the drawings in the embodiments of the present invention, but it should be understood that the scope of the present invention is not limited by the specific embodiments.
Example 1
As shown in fig. 1-3, a system for measuring the area of a plant leaf based on a photosynthetic apparatus is provided on the photosynthetic apparatus, and comprises:
the infrared thermal imaging temperature measurement camera system is arranged below a primary leaf chamber on the photosynthetic instrument and is used for acquiring image information of plant leaves to be measured in real time;
the auxiliary leaf chamber is arranged between the bottom of the original leaf chamber on the photosynthetic apparatus and the infrared thermal imaging temperature measurement camera system, one end of the top of the auxiliary leaf chamber is hermetically communicated with the bottom of the original leaf chamber on the photosynthetic apparatus, and one end of the bottom of the auxiliary leaf chamber is hermetically covered on the infrared thermal imaging temperature measurement camera system;
and the picture information processing module is used for processing the image information of the plant leaf to be measured, which is acquired by the infrared thermal imaging temperature measurement camera system, so as to obtain the leaf area information and the leaf temperature information of the plant leaf to be measured.
The plant leaf area measuring system creatively solves the technical defect that the LI-6400/6800 photosynthetic instrument cannot measure the leaf area by additionally arranging the auxiliary leaf chamber at the lower part of the primary leaf chamber of the LI-6400 photosynthetic instrument and arranging the infrared thermal imaging temperature measurement camera system for acquiring the leaf image information of the plant to be measured in the auxiliary leaf chamber.
In addition, the auxiliary leaf chamber is arranged at the bottom of the original leaf chamber on the photosynthetic apparatus, so that the problem of installation of an infrared thermal imaging temperature measurement camera system for acquiring leaf image information is solved, the air tightness of the original leaf chamber of the LI-6400/6800 photosynthetic apparatus is ensured, and the measurement condition is met. The method comprises the steps of placing plant leaves to be measured in a primary leaf chamber and an auxiliary leaf chamber on a communicated photosynthetic instrument, acquiring image information of the plant leaves to be measured by using an infrared thermal imaging temperature measurement camera system, transmitting the acquired image information to a picture information processing module by using the infrared thermal imaging temperature measurement camera system, processing and analyzing the received picture information by using the picture information processing module, and obtaining leaf area information and leaf temperature information of the plant leaves to be measured according to a processing result.
The auxiliary leaf chamber is in a rectangular column shape, the cross section of the auxiliary leaf chamber is a rectangular surface of 3cm multiplied by 3cm, the auxiliary leaf chamber is in sealed connection with the original leaf chamber on the photosynthesis instrument conveniently, and a measuring leaf chamber of the plant leaf is formed.
The top of the auxiliary leaf chamber is an opening, and the opening at the top is sleeved at the bottom of the original leaf chamber on the photosynthetic instrument and is communicated with the bottom of the original leaf chamber on the photosynthetic instrument in a sealing way. In order to realize the sealing connection between the top of the auxiliary leaf chamber and the bottom of the primary leaf chamber on the photosynthetic instrument, a sealing gasket can be arranged between the top of the auxiliary leaf chamber and the bottom of the primary leaf chamber on the photosynthetic instrument.
The bottom surface of the auxiliary leaf chamber is provided with a mounting groove, a camera lens of the infrared thermal imaging temperature measurement camera system is positioned in the mounting groove and faces the top opening of the auxiliary leaf chamber, and the outer wall of the camera lens of the infrared thermal imaging temperature measurement camera system is connected with the mounting groove in a sealing mode. In order to meet the sealing condition when the plant leaf area is measured, the infrared thermal imaging temperature measurement camera system and the auxiliary leaf chamber can be connected in a sealing mode through the sealing ring.
The distance between the camera lens of the infrared thermal imaging temperature measurement camera system and the top opening of the auxiliary leaf chamber is more than 20cm. Because a certain photographing distance is needed between a camera lens in the infrared thermal imaging temperature measurement camera system and a photographed object, a clear infrared image can be photographed about 20cm away from a target object, so that the distance between the camera lens of the infrared thermal imaging temperature measurement camera system and the top opening of the auxiliary leaf chamber is more than 20cm, and the camera shooting condition of the infrared thermal imaging temperature measurement camera system is met.
The auxiliary vane chamber is made of aluminum lithium alloy. The auxiliary blade chamber is made of the aluminum-lithium alloy, so that the overall strength of the auxiliary blade chamber is increased, the auxiliary blade chamber has good tightness, corrosion resistance, fatigue resistance and proper ductility, and the actual measurement requirement is met.
The infrared thermal imaging temperature measurement camera system is in communication connection with the picture information processing module, and sends the image information of the plant leaves to be measured, which is shot in real time, to the picture information processing module, and the picture information processing module performs specific processing and analysis according to the image information of the plant leaves shot by the infrared thermal imaging temperature measurement camera system, so that the leaf area information and the leaf temperature information of the plant leaves to be measured can be obtained.
When the image information processing module processes and analyzes the shot image information of the plant leaves and obtains the leaf area information of the plant leaves to be measured, imageJ software is specifically adopted for processing, and the processing process is as follows:
(1) Open ruler picture using ImageJ, note: the ruler picture needs to be taken under the same condition as the blade infrared picture.
(2) The ruler is marked using a straight tool. Setting a scale: a menu bar-Analyze-Setscale, enters the corresponding unit and length values in a pop-up dialog box.
(3) Rectangular leaf, opening the infrared image of leaf, selecting Rectangular leaf, and drawing the shape of leaf. And clicking the analysis-measure to obtain the area of the measured leaf.
(4) For other shapes of blades, freehand electricities were chosen to draw the blade profile along the blade edge. And clicking the analysis-measure to obtain the area of the leaf.
The left picture of fig. 4 is a picture shot by a camera, the right picture of fig. 4 is an infrared leaf picture shot by the plant leaf area measuring system of the photosynthesis apparatus, and the two pictures are compared to find that the edge of the leaf picture shot by infrared is clear and is not influenced by a background light source. The common camera is affected by the light source, and the effect is poor.
When the image information processing module processes and analyzes the image information of the shot plant leaves and obtains the leaf temperature information of the plant leaves to be measured, the specific processing process is as follows:
the infrared thermal imaging temperature measurement camera system in the application document utilizes a thermal imaging technology to convert the temperature difference of plant leaves to be measured into a real-time video image to be displayed, and the specific infrared thermal imaging technology is that a micro thermal radiation detector, an optical imaging objective lens and an optical machine scanning system are utilized to receive an infrared radiation signal of a measured object, a focused infrared radiation energy distribution pattern is reflected to a photosensitive element of the infrared detector through spectral filtering and spatial filtering, namely, an infrared thermal image of the measured object is scanned and focused on a unit or a light splitting detector, the detector converts the infrared radiation energy into an electric signal, the electric signal is amplified and converted into a standard video signal, and the standard video signal is displayed on an infrared thermal image through a television screen or a monitor.
The infrared thermal imaging temperature measurement camera system converts infrared radiation energy emitted by the blade into an electric signal, the size of the infrared radiation energy corresponds to the surface temperature of the blade, and the surface temperature of the blade can be determined according to the size of the converted electric signal. As shown in fig. 5, the leaf temperature information in the image information of the photographed plant leaf obtained by the picture information processing module.
According to the invention, the original leaf chamber on the photosynthetic apparatus is improved, and the infrared thermal imaging temperature measurement camera system is added, so that the real-time measurement of the leaf area and the leaf temperature can be realized, and the measurement accuracy of the photosynthetic apparatus is greatly improved. The photosynthetic apparatus can measure smaller leaves, and the data credibility, applicability and convenience of the LI-6400/6800 can be greatly improved. Meanwhile, the plant leaf area measuring system realizes temperature measurement on the plant leaf area and nondestructive measurement on the leaf area by adopting the thermal imaging technology of the infrared thermal imaging temperature measurement camera system, realizes tracking of limited samples or long-term tests of growth, leaf expansion or stress resistance of the same plant, and avoids the problem that adaptive leaf chambers need to be replaced when measuring the leaves with different sizes.
Example 2
The invention provides a method for using a plant leaf area measuring system based on a photosynthetic apparatus, which comprises the following steps:
arranging an infrared thermal imaging temperature measurement camera system below a primary leaf chamber on a photosynthetic instrument;
arranging an auxiliary leaf chamber between the bottom of a primary leaf chamber on a photosynthetic instrument and an infrared thermal imaging temperature measurement camera system, and hermetically communicating one end of the top of the auxiliary leaf chamber with the bottom of the primary leaf chamber on the photosynthetic instrument, wherein one end of the bottom is hermetically covered on the infrared thermal imaging temperature measurement camera system;
placing plant leaves to be measured in an auxiliary leaf chamber, and acquiring image information of the plant leaves to be measured by using an infrared thermal imaging temperature measurement camera system;
the infrared thermal imaging temperature measurement camera system transmits the acquired image information to the picture information processing module, and the picture information processing module processes the acquired image information to obtain the leaf area information and the leaf temperature information of the plant leaves to be measured.
Finally, the description is as follows: the above disclosure is only one specific embodiment of the present invention, however, the present invention is not limited thereto, and any modifications that can be made by those skilled in the art should fall within the protection scope of the present invention.
Claims (7)
1. The utility model provides a plant leaf area measurement system based on photosynthetic apparatus establishes on photosynthetic apparatus which characterized in that includes:
the infrared thermal imaging temperature measurement camera system is arranged below an original leaf chamber on the photosynthetic instrument and is used for acquiring image information of plant leaves to be measured in real time;
the auxiliary leaf chamber is arranged between the bottom of the original leaf chamber on the photosynthetic apparatus and the infrared thermal imaging temperature measurement camera system, one end of the top of the auxiliary leaf chamber is hermetically communicated with the bottom of the original leaf chamber on the photosynthetic apparatus, and one end of the bottom of the auxiliary leaf chamber is hermetically covered on the infrared thermal imaging temperature measurement camera system;
and the picture information processing module is used for processing the image information of the plant leaves to be measured, which is acquired by the infrared thermal imaging temperature measurement camera system, so as to obtain the leaf area information and the leaf temperature information of the plant leaves to be measured.
2. A photosynthesis instrument based plant leaf area measurement system according to claim 1, wherein: the auxiliary leaf chamber is in a rectangular column shape, and the cross section of the auxiliary leaf chamber is a rectangular surface of 3cm multiplied by 3 cm.
3. A photosynthesis instrument based plant leaf area measurement system according to claim 2, wherein: the top of the auxiliary leaf chamber is provided with an opening, and the opening at the top is sleeved at the bottom of the original leaf chamber on the photosynthetic instrument and is communicated with the bottom of the original leaf chamber on the photosynthetic instrument in a sealing way;
the bottom surface of the auxiliary blade chamber is provided with an installation groove;
the camera lens of the infrared thermal imaging temperature measurement camera system is located in the mounting groove and faces the top opening of the auxiliary leaf chamber, and the outer wall of the camera lens of the infrared thermal imaging temperature measurement camera system is connected with the mounting groove in a sealing mode.
4. A photosynthesis instrument based plant leaf area measurement system according to claim 3, wherein: the distance between a camera lens of the infrared thermal imaging temperature measurement camera system and the top opening of the auxiliary leaf chamber is larger than 20cm.
5. A photosynthesis instrument based plant leaf area measurement system according to claim 1, wherein: the auxiliary leaf chamber is made of aluminum lithium alloy.
6. A photosynthesis instrument based plant leaf area measurement system according to claim 1, wherein: the infrared thermal imaging temperature measurement camera system sends image information of the plant leaves to be measured, which is shot in real time, to the picture information processing module, and the picture information processing module processes and analyzes the image information to obtain leaf area information and leaf temperature information of the plant leaves to be measured.
7. Use of a photosynthesis instrument based plant leaf area measurement system according to any one of claims 1 to 6, wherein: the method comprises the following steps:
arranging an infrared thermal imaging temperature measurement camera system below a primary leaf chamber on a photosynthetic instrument;
arranging an auxiliary leaf chamber between the bottom of a primary leaf chamber on a photosynthetic instrument and an infrared thermal imaging temperature measurement camera system, and hermetically communicating one end of the top of the auxiliary leaf chamber with the bottom of the primary leaf chamber on the photosynthetic instrument, wherein one end of the bottom is hermetically covered on the infrared thermal imaging temperature measurement camera system;
placing plant leaves to be measured in an auxiliary leaf chamber, and acquiring image information of the plant leaves to be measured by using an infrared thermal imaging temperature measurement camera system;
the infrared thermal imaging temperature measurement camera system transmits the acquired image information to the picture information processing module, and the picture information processing module processes the acquired image information to obtain leaf area information and leaf temperature information of the plant leaves to be measured.
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