CN115305201B - Microbial agent for efficiently treating oily sludge and preparation method thereof - Google Patents
Microbial agent for efficiently treating oily sludge and preparation method thereof Download PDFInfo
- Publication number
- CN115305201B CN115305201B CN202211126956.7A CN202211126956A CN115305201B CN 115305201 B CN115305201 B CN 115305201B CN 202211126956 A CN202211126956 A CN 202211126956A CN 115305201 B CN115305201 B CN 115305201B
- Authority
- CN
- China
- Prior art keywords
- parts
- weight
- culture medium
- oily sludge
- bacterial
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
Abstract
The invention belongs to the technical field of sludge treatment, and particularly relates to a microbial agent for efficiently treating oily sludge and a preparation method thereof. According to the invention, three different culture mediums are designed, the oily sludge is inoculated to the different culture mediums, and the bacterial liquid cultured by the culture medium A can increase the surface activity of the oily sludge, so that the oily sludge can be eluted better, and the bacterial liquid cultured by the culture medium B, C can take the oil in the oily sludge as a carbon source, so that the sludge can be effectively degraded. The microbial inoculum is obtained by compounding the three bacterial liquids, can efficiently elute oily sludge and degrade and remove petroleum pollutants within 4-7 days, has simple preparation process and controllable culture conditions, is easy to realize, and is suitable for industrialized popularization.
Description
Technical Field
The invention belongs to the technical field of sludge treatment, and particularly relates to a microbial agent for efficiently treating oily sludge and a preparation method thereof.
Background
The petroleum industry generates a large amount of oily sludge during the exploration, production, transportation, storage and refining of crude oil, and the treatment of oily sludge is a problem, and has been receiving more and more attention in recent years. Oily sludge contains high concentrations of Petroleum Hydrocarbons (PHCs) and other difficult to degrade components, is considered a hazardous waste in many countries, and mishandling or under-treatment can pose serious threats to the environment and human health, and effective remediation has become a worldwide problem due to the ever-increasing harmfulness and yield of oily sludge. Over the years, various oily sludge treatment processes have been developed, such as farming, incineration, solidification/stabilization, solvent extraction, ultrasonic treatment, pyrolysis, photocatalysis, chemical treatment, biodegradation, and the like. By using these techniques, the content of hazardous components can be reduced or eliminated, thereby mitigating their detrimental effects on the environment and health.
The biological treatment eliminates pollutants by utilizing the action of soil origin or exogenous microorganisms, repairs petroleum polluted environment by accelerating the degradation of PHCs by microorganisms, has simple operation, does not need to be managed and can not produce secondary pollutants, can conform to the strategy of sustainable development, and is focused by a plurality of scholars in the field of environmental microorganisms. Therefore, development of some microbial agents for efficiently treating oily sludge is urgently required.
Disclosure of Invention
In order to solve the technical problems, the invention adopts different culture mediums to carry out screening culture on microbial agents in the oily sludge, so as to respectively obtain microbial bacterial liquid capable of promoting the surface activity of the oily sludge and effectively degrading the oily sludge, and realize the efficient degradation of the oily sludge.
In order to achieve the above purpose, the embodiment of the invention provides a preparation method of a microbial agent for efficiently treating oil-containing sludge, which specifically comprises the following steps:
respectively inoculating the oily sludge on a culture medium A, a culture medium B and a culture medium C for culturing to obtain a bacterial solution A, a bacterial solution B and a bacterial solution C;
and (2) mixing the bacterial solution A, the bacterial solution B and the bacterial solution C according to the ratio of 0.1-10:0.1-10: mixing the materials according to the proportion of 0.1-10 to obtain a microbial agent;
the culture medium A is as follows: glucose 1-10 parts, yeast extract 0.1-10 parts, urea 1-10 parts, K 2 HPO 4 ·3H 2 O1-10 parts, KH 2 PO 4 1-10 parts of MgSO 4 ·7H 2 1 to 10 parts of O and 1 to 10 parts of microelement liquid; 1000 parts of deionized water;
the culture medium B is as follows: naNO 3 1 to 15 parts of K 2 HPO 4 ·3H 2 O1-10 parts, KH 2 PO 4 1-10 parts of MgSO 4 ·7H 2 0.1 to 1 part of O, 1 to 10 parts of NaCl, 1 to 10 parts of KCl and CaCl 2 0.01 to 1 part of FeCl 3 0.01 to 1 part of deionized water 1000 parts;
the culture medium C is as follows: NH (NH) 4 Cl 0.1-1 parts, K 2 HPO 4 ·3H 2 O1-10 parts, KH 2 PO 4 1-10 parts of MgSO 4 ·7H 2 0.1 to 1 part of O, 1 to 10 parts of NaCl, 1 to 10 parts of KCl and CaCl 2 0.01 to 1 part of FeCl 3 0.01-1 part and 1000 parts of deionized water.
Further, the trace element liquid is: znSO (ZnSO) 4 ·7H 2 0.1 to 10 portions of O and MnSO 4 ·H 2 0.1 to 10 portions of O and FeSO 4 ·7H 2 0.1 to 10 portions of O and CaCl 2 ·2H 2 O0.01-1 part, coCl 2 0.01 to 1 part of 6H2O and CuSO 4 ·5H 2 0.01 to 1 part of O, H 3 BO 3 0.01 to 1 part of Na 2 MoO 4 0.01 to 1 part of H2O and 1000 parts of deionized water.
Further, the inoculation amount of the oil-containing sludge is 1-5%.
Further, the technological parameters of the culture process are as follows: culture temperature: 25-35 ℃; rotation speed of the cradle: 110-200r/min, culture time: 2-7 days.
Further, the pH value of the culture medium A is 7.0-7.5 after the culture medium A is configured, and the culture medium A is sterilized by high-pressure steam for 30min at 115 ℃; the pH value of the B, C culture medium is 7.0-7.5 after the culture medium is prepared, and the culture medium is sterilized by high-pressure steam for 20min at the temperature of 121 ℃.
Further, the concentration of the A bacterial liquid, the B bacterial liquid and the C bacterial liquid is more than or equal to 10 8 /mL。
Based on the same inventive concept, the embodiment of the invention also provides a microbial agent for efficiently treating the oil-containing sludge, and the microbial agent is prepared by the preparation method.
The beneficial effects are that:
according to the invention, different culture mediums are designed, the oily sludge is inoculated to the different culture mediums, and the bacterial liquid cultured by the culture medium A can increase the surface activity of the oily sludge, so that the oily sludge can be eluted better, and the bacterial liquid cultured by the culture medium B, C can take the oil in the oily sludge as a carbon source, so that the sludge can be effectively degraded. The three bacterial solutions are compounded according to a certain proportion, so that the oily sludge can be efficiently eluted within 4-7 days and the petroleum pollutants can be degraded and removed. The preparation process of the microbial inoculum is simple, the culture condition is controllable and easy to realize, and the microbial inoculum is suitable for industrialized popularization.
Detailed Description
For a clearer explanation of the technical content of the present invention, reference is made to the detailed description herein with reference to specific examples, which are, obviously, only preferred embodiments of the present technical solution, and other technical solutions that will be apparent to those skilled in the art from the disclosed technical content still fall within the scope of the present invention.
In the embodiment of the invention, the chemical reagent can be obtained by purchasing or preparing by the existing preparation method, and the adopted instrument equipment is conventional equipment in the prior art.
Example 1
The preparation of a culture medium A, weighing the following raw materials in parts by weight: glucose 5 weight parts, yeast extract 1 (Yeast extract) 1 weight parts, urea 1.5 weight parts, K 2 HPO 4 ·3H 2 O1.5 weight parts, KH 2 PO 4 1.5 parts by weight of MgSO 4 ·7H 2 0.5 part by weight of O, 1 part by weight of NaCl, 1 part by weight of KCl, 1 part by weight of microelement liquid and 1000 parts by weight of deionized water, stirring and dissolving, and sterilizing for 30min under high pressure steam at 115 ℃. Wherein the microelements are prepared as follows: znSO (ZnSO) 4 ·7H 2 O0.1 weight part, mnSO 4 ·H 2 O0.25 weight part, feSO 4 ·7H 2 O0.1 part by weight, caCl 2 ·2H 2 O0.01 part by weight, coCl 2 6H2O 0.1 part by weight, cuSO 4 ·5H 2 O0.1 part by weight, H 3 BO 3 0.01 part by weight of Na 2 MoO 4 ·H 2 0.02 parts of O and 1000 parts of deionized water.
B, preparing a culture medium, and weighing the following raw materials in parts by weight: NH (NH) 4 Cl 0.8 part by weight, K 2 HPO 4 ·3H 2 O1 weight part, KH 2 PO 4 1 part by weight of MgSO 4 ·7H 2 O0.5 weight portions, naCl 1 weight portions, KCl 1 weight portionsParts by weight, caCl 2 0.02 part by weight of FeCl 3 0.01 part by weight of deionized water 1000 parts by weight, pH 7.2 after stirring and dissolution, and sterilizing with high-pressure steam at 121 ℃ for 20min.
C, preparing a culture medium, and weighing the following raw materials in parts by weight: naNO 3 1.3 parts by weight, K 2 HPO 4 ·3H 2 O1 weight part, KH 2 PO 4 1 part by weight of MgSO 4 ·7H 2 0.5 part by weight of O, 1 part by weight of NaCl, 1 part by weight of KCl and CaCl 2 0.02 part by weight of FeCl 3 0.01 part by weight of deionized water 1000 parts by weight, pH 7.2 after stirring and dissolution, and sterilizing with high-pressure steam at 121 ℃ for 20min.
Collecting oily sludge of Liaohe oil field as inoculum to prepare bacterial liquid suspension, inoculating the oily sludge into A, B, C three culture mediums according to the inoculum size of 3%, culturing in a shaking table at the speed of 180r/min at 30 ℃ for 7 days, and obtaining corresponding bacterial liquid, namely A bacterial liquid, B bacterial liquid and C bacterial liquid when the bacterial liquid concentration in each culture medium reaches 108/mL.
Taking 1 part by weight of the A bacterial liquid, 1 part by weight of the B bacterial liquid and 1 part by weight of the C bacterial liquid, and mixing to obtain the microbial agent.
Experiments are carried out on oily sludge (oil content is 5-20%) retrieved from Liaohe oil field, 150g of oily sludge is pretreated with water according to a certain proportion, and 5 experimental groups are set, namely a control group (without adding strain), a group A (adding strain A), a group B (adding strain B), a group C (adding strain C) and a group D (microbial agent obtained in example 1); the inoculation amount of the A, B, C, D group of bacteria agents is 2 percent; wherein the oily sludge after 7 days turns yellow obviously, the black viscosity becomes less, and the removal rate of petroleum hydrocarbon pollutants is shown in table 1.
TABLE 1
| Experimental group | Control group | Group A | Group B | Group C | Group D |
| Raw oil content/g.kg -1 | 150.6 | 158.5 | 152.5 | 155.7 | 160.4 |
| Oil content after treatment/g.kg -1 | 145.3 | 105.1 | 81.5 | 89.5 | 63.3 |
| Removal rate% | 3.5 | 34.6 | 46.5 | 42.5 | 60.5 |
Example 2
The preparation of a culture medium A, weighing the following raw materials in parts by weight: glucose 3 weight parts, yeast extract 0.6 weight parts, urea 1.2 weight parts, K 2 HPO 4 ·3H 2 O2 weight portion, KH 2 PO 4 2 parts by weight of MgSO 4 ·7H 2 0.3 part by weight of O, 2 parts by weight of NaCl, 2 parts by weight of KCl, 2 parts by weight of microelement liquid and 1000 parts by weight of deionized water, stirring and dissolving, and sterilizing for 30min under high pressure steam at 115 ℃. Wherein the microelements are prepared as follows: znSO (ZnSO) 4 ·7H 2 O0.2 weight part, mnSO 4 ·H 2 O0.5 weight part, feSO 4 ·7H 2 O0.5 weight part, caCl 2 ·2H 2 O0.05 part by weight, coCl 2 6H2O 0.5 part by weight, cuSO 4 ·5H 2 O0.5 weight part, H 3 BO 3 0.03 part by weight of Na 2 MoO 4 ·H 2 0.05 parts of O and 1000 parts of deionized water.
B, preparing a culture medium, and weighing the following raw materials in parts by weight: NH (NH) 4 Cl 0.6 part by weight, K 2 HPO 4 ·3H 2 O3 weight portions, KH 2 PO 4 2 parts by weight of MgSO 4 ·7H 2 0.8 part by weight of O, 2 parts by weight of NaCl, 2 parts by weight of KCl and CaCl 2 0.06 part by weight of FeCl 3 0.04 parts by weight of deionized water 1000 parts by weight, pH 7.4 after stirring and dissolution, and high pressure steam sterilization at 121 ℃ for 20min.
C, preparing a culture medium, and weighing the following raw materials in parts by weight: naNO 3 1.6 parts by weight, K 2 HPO 4 ·3H 2 O2 weight portions, KH 2 PO 4 2 parts by weight of MgSO 4 ·7H 2 O1 weight part, naCl 2 weight part, KCl 2 weight part and CaCl 2 0.04 part by weight of FeCl 3 0.03 weight part, 1000 weight parts of deionized water, stirring and dissolving, and sterilizing for 20min under high pressure steam at 121 ℃.
Collecting oily sludge from Liaohe oil field as inoculum to prepare bacterial suspension, inoculating the oily sludge into A, B, C three culture mediums according to 2% inoculum size, culturing in shaking table at 28deg.C and 150r/min for 7 days, and culturing bacterial suspension in each culture medium until bacterial concentration reaches 10 8 And (3) obtaining corresponding bacterial solutions, namely bacterial solution A, bacterial solution B and bacterial solution C.
Taking 3 parts by weight of the bacterial liquid A, 2 parts by weight of the bacterial liquid B and 3 parts by weight of the bacterial liquid C, and mixing to obtain the microbial agent.
Experiments are carried out on oily sludge (oil content is 5-20%) retrieved from Liaohe oil field, 150g of oily sludge is pretreated with water according to a certain proportion, and 5 experimental groups are set, namely a control group (without adding strain), a group A (adding strain A), a group B (adding strain B), a group C (adding strain C) and a group D (microbial agent obtained in example 2); the inoculation amount of the A, B, C, D group of bacteria agents is 2 percent; the oily sludge became significantly yellow and black viscous and less after 7 days, and the petroleum hydrocarbon pollutant removal rates were as shown in table 2.
TABLE 2
| Experimental group | Control group | Group A | Group B | Group C | Group D |
| Raw oil content/g.kg -1 | 155.6 | 157.6 | 153.5 | 157.6 | 162.1 |
| Oil content after treatment/g.kg -1 | 151.3 | 104.9 | 83.7 | 89.1 | 59.3 |
| Removal rate% | 2.8 | 33.4 | 45.5 | 43.5 | 63.4 |
Example 3
The preparation of a culture medium A, weighing the following raw materials in parts by weight: glucose 7 weight parts, yeast extract 5 weight parts, urea 3 weight parts, K 2 HPO 4 ·3H 2 O2.5 weight portions, KH 2 PO 4 2.5 parts by weight of MgSO 4 ·7H 2 0.5 part by weight of O, 2.5 parts by weight of NaCl, 2.5 parts by weight of KCl, 2.5 parts by weight of microelement liquid and 1000 parts by weight of deionized water, stirring and dissolving, and sterilizing for 30min under high pressure steam at 115 ℃. Wherein the microelements are prepared as follows: znSO (ZnSO) 4 ·7H 2 O0.4 weight part, mnSO 4 ·H 2 O0.7 weight part, feSO 4 ·7H 2 O0.7 weight portions, caCl 2 ·2H 2 O0.07 part by weight, coCl 2 6H2O 0.7 parts by weight, cuSO 4 ·5H 2 O0.7 weight part, H 3 BO 3 0.05 part by weight of Na 2 MoO 4 ·H 2 O0.07 weight portions, deionized water 1000 weight portions.
B, preparing a culture medium, and weighing the following raw materials in parts by weight: NH (NH) 4 Cl 0.75 weight portions, K 2 HPO 4 ·3H 2 O5 weight portions, KH 2 PO 4 3 parts by weight of MgSO 4 ·7H 2 O0.5 weight3.5 parts by weight of NaCl, 3.5 parts by weight of KCl and CaCl 2 0.12 part by weight of FeCl 3 0.08 weight part, 1000 weight parts of deionized water, stirring and dissolving, and sterilizing by high-pressure steam at 121 ℃ for 20min.
C, preparing a culture medium, and weighing the following raw materials in parts by weight: naNO 3 2.4 parts by weight, K 2 HPO 4 ·3H 2 O3.5 weight portions, KH 2 PO 4 3.5 parts by weight of MgSO 4 ·7H 2 0.7 part by weight of O, 3.5 parts by weight of NaCl, 3.5 parts by weight of KCl and CaCl 2 0.12 part by weight of FeCl 3 0.14 weight parts, 1000 weight parts of deionized water, stirring and dissolving, and sterilizing with high pressure steam at 121 ℃ for 20min.
Collecting oily sludge from Liaohe oil field as inoculum to prepare bacterial suspension, inoculating the oily sludge into A, B, C three culture mediums according to 5% inoculum size, culturing in shaking table at 33deg.C and 190r/min for 6 days, and culturing bacterial suspension in each culture medium until bacterial concentration reaches 10 8 And (3) obtaining corresponding bacterial solutions, namely bacterial solution A, bacterial solution B and bacterial solution C.
And 5 parts by weight of the A bacterial liquid, 4 parts by weight of the B bacterial liquid and 5 parts by weight of the C bacterial liquid are mixed to obtain the microbial agent.
Experiments are carried out on oily sludge (oil content is 5-20%) retrieved from Liaohe oil field, 150g of oily sludge is pretreated with water according to a certain proportion, and 5 experimental groups are set, namely a control group (without adding strain), a group A (adding strain A), a group B (adding strain B), a group C (adding strain C) and a group D (microbial agent obtained in example 3); the inoculation amount of the A, B, C, D group of bacteria agents is 2 percent; the removal rate of petroleum hydrocarbon contaminants after 6 days is shown in table 3.
TABLE 3 Table 3
Experiments show that the removal rate of the three bacterial agents inoculated with A, B, C on petroleum pollutants in the oil-containing sludge is less than 50%, and the removal rate of the pollutants in the oil-containing sludge in the experimental group inoculated with the mixed bacterial liquid added in proportion is far higher than that of other experimental groups. After the subsequent physicochemical treatment is carried out, the oil content of the bottom sludge in the oil-containing sludge is less than 1%, and the COD (chemical oxygen demand) in the supernatant fluid is less than 9, so that the harmless treatment of the oil-containing sludge is realized.
The above embodiments are only preferred embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be able to apply equivalents and modifications according to the technical solution and the concept of the present invention within the scope of the present invention.
Claims (4)
1. The preparation method of the microbial agent for efficiently treating the oily sludge is characterized by comprising the following steps of:
respectively inoculating the oily sludge on a culture medium A, a culture medium B and a culture medium C for culturing to obtain a bacterial solution A, a bacterial solution B and a bacterial solution C; wherein, the technological parameters of the culture process are as follows:
culture temperature: 25-35 ℃; rotation speed of the cradle: 110-200r/min, culture time: 2-7 days;
the pH value of the culture medium A is 7.0-7.5 after the culture medium A is prepared, and the culture medium A is sterilized by high-pressure steam for 30min at 115 ℃; the pH value of the B, C culture medium is 7.0-7.5 after the culture medium is prepared, and the culture medium is sterilized by high-pressure steam for 20min at 121 ℃;
and (2) mixing the bacterial solution A, the bacterial solution B and the bacterial solution C according to the ratio of 0.1-10:0.1-10: mixing the materials according to the proportion of 0.1-10 to obtain a microbial agent;
the culture medium A is as follows: glucose 1-10 parts, yeast extract 0.1-10 parts, urea 1-10 parts, K 2 HPO 4 ·3H 2 O1-10 parts, KH 2 PO 4 1-10 parts of MgSO 4 ·7H 2 1 to 10 parts of O and 1 to 10 parts of microelement liquid; 1000 parts of deionized water; wherein the microelement liquid is as follows: znSO (ZnSO) 4 ·7H 2 0.1 to 10 portions of O and MnSO 4 ·H 2 O 0.1About 10 parts of FeSO 4 ·7H 2 0.1 to 10 portions of O and CaCl 2 ·2H 2 O0.01-1 part, coCl 2 0.01 to 1 part of 6H2O and CuSO 4 ·5H 2 0.01 to 1 part of O, H 3 BO 3 0.01 to 1 part of Na 2 MoO 4 0.01 to 1 part of H2O and 1000 parts of deionized water;
the culture medium B is as follows: naNO 3 1 to 15 parts of K 2 HPO 4 ·3H 2 O1-10 parts, KH 2 PO 4 1-10 parts of MgSO 4 ·7H 2 0.1 to 1 part of O, 1 to 10 parts of NaCl, 1 to 10 parts of KCl and CaCl 2 0.01 to 1 part of FeCl 3 0.01 to 1 part of deionized water 1000 parts;
the culture medium C is as follows: NH (NH) 4 Cl 0.1-1 parts, K 2 HPO 4 ·3H 2 O1-10 parts, KH 2 PO 4 1-10 parts of MgSO 4 ·7H 2 0.1 to 1 part of O, 1 to 10 parts of NaCl, 1 to 10 parts of KCl and CaCl 2 0.01 to 1 part of FeCl 3 0.01-1 part and 1000 parts of deionized water.
2. The method for preparing a microbial agent for efficiently treating oily sludge according to claim 1, wherein the inoculum size of the oily sludge is 1-5%.
3. The method for preparing a microbial agent for efficiently treating oily sludge according to claim 1, wherein the concentration of the A bacterial liquid, the B bacterial liquid and the C bacterial liquid is 10 or more 8 /mL。
4. The microbial agent for efficiently treating the oily sludge is characterized by being prepared by the preparation method of any one of claims 1-3.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211126956.7A CN115305201B (en) | 2022-09-16 | 2022-09-16 | Microbial agent for efficiently treating oily sludge and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211126956.7A CN115305201B (en) | 2022-09-16 | 2022-09-16 | Microbial agent for efficiently treating oily sludge and preparation method thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115305201A CN115305201A (en) | 2022-11-08 |
| CN115305201B true CN115305201B (en) | 2023-09-22 |
Family
ID=83866095
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211126956.7A Active CN115305201B (en) | 2022-09-16 | 2022-09-16 | Microbial agent for efficiently treating oily sludge and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115305201B (en) |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690939A (en) * | 2009-07-13 | 2010-04-07 | 延安市微生物研究所 | Method for degrading crude oil by separating compound bacteria solution from contaminated fossil oil sample |
| CN103865855A (en) * | 2014-03-25 | 2014-06-18 | 南京工业大学 | Bacillus subtilis strain and applications thereof |
| CN104388312A (en) * | 2014-12-10 | 2015-03-04 | 青岛农业大学 | Screening method for petroleum degrading bacteria, method for preparing petroleum degrading bacteria inoculant from screened bacteria, and application of inoculant |
| CN104450597A (en) * | 2014-12-10 | 2015-03-25 | 青岛农业大学 | Preparation method of petroleum degrading bacteria solid inoculant and method for restoring petroleum-polluted soil by using prepared solid inoculant |
| CN106929419A (en) * | 2017-04-12 | 2017-07-07 | 临沂大学 | A kind of screening technique of anaerobic degradation petroleum hydrocarbon methanogen floras |
| CN106929420A (en) * | 2017-04-12 | 2017-07-07 | 临沂大学 | A kind of method of decomposing petroleum hydrocarbon |
| CN107201311A (en) * | 2017-04-12 | 2017-09-26 | 临沂大学 | A kind of screening technique of anaerobic degradation petroleum hydrocarbon methanogen floras |
| CN107338206A (en) * | 2017-07-24 | 2017-11-10 | 青岛金典生化器材有限公司 | A kind of culture medium for being enriched with Bacillus acidi lactici and preparation method thereof |
| CN109161499A (en) * | 2018-08-29 | 2019-01-08 | 华南理工大学 | A kind of application for producing surfactant bacterium and its cutting down polycyclic aromatic hydrocarbon in situ in coal/petrochemical wastewater |
| CN109401974A (en) * | 2018-12-05 | 2019-03-01 | 河北省地质环境监测院 | A kind of oil-polluted soils microbial treatment method |
| CN109777759A (en) * | 2019-03-20 | 2019-05-21 | 天津瑞达同方生物科技开发有限公司 | It is a kind of for oily sludge and the processing microbial inoculum of sewage and preparation method thereof |
| CN111748483A (en) * | 2019-03-29 | 2020-10-09 | 中国科学院沈阳应用生态研究所 | Bacillus for degrading petroleum hydrocarbon and application thereof |
| CN113265362A (en) * | 2021-06-23 | 2021-08-17 | 中南大学 | Composite microbial inoculum for efficiently converting soil heavy metal chromium as well as preparation method and application thereof |
| CN114480202A (en) * | 2022-02-16 | 2022-05-13 | 湖北茂盛生物有限公司 | Bioremediation microbial inoculum for petroleum-polluted soil, preparation device and method |
-
2022
- 2022-09-16 CN CN202211126956.7A patent/CN115305201B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690939A (en) * | 2009-07-13 | 2010-04-07 | 延安市微生物研究所 | Method for degrading crude oil by separating compound bacteria solution from contaminated fossil oil sample |
| CN103865855A (en) * | 2014-03-25 | 2014-06-18 | 南京工业大学 | Bacillus subtilis strain and applications thereof |
| CN104388312A (en) * | 2014-12-10 | 2015-03-04 | 青岛农业大学 | Screening method for petroleum degrading bacteria, method for preparing petroleum degrading bacteria inoculant from screened bacteria, and application of inoculant |
| CN104450597A (en) * | 2014-12-10 | 2015-03-25 | 青岛农业大学 | Preparation method of petroleum degrading bacteria solid inoculant and method for restoring petroleum-polluted soil by using prepared solid inoculant |
| CN106929419A (en) * | 2017-04-12 | 2017-07-07 | 临沂大学 | A kind of screening technique of anaerobic degradation petroleum hydrocarbon methanogen floras |
| CN106929420A (en) * | 2017-04-12 | 2017-07-07 | 临沂大学 | A kind of method of decomposing petroleum hydrocarbon |
| CN107201311A (en) * | 2017-04-12 | 2017-09-26 | 临沂大学 | A kind of screening technique of anaerobic degradation petroleum hydrocarbon methanogen floras |
| CN107338206A (en) * | 2017-07-24 | 2017-11-10 | 青岛金典生化器材有限公司 | A kind of culture medium for being enriched with Bacillus acidi lactici and preparation method thereof |
| CN109161499A (en) * | 2018-08-29 | 2019-01-08 | 华南理工大学 | A kind of application for producing surfactant bacterium and its cutting down polycyclic aromatic hydrocarbon in situ in coal/petrochemical wastewater |
| CN109401974A (en) * | 2018-12-05 | 2019-03-01 | 河北省地质环境监测院 | A kind of oil-polluted soils microbial treatment method |
| CN109777759A (en) * | 2019-03-20 | 2019-05-21 | 天津瑞达同方生物科技开发有限公司 | It is a kind of for oily sludge and the processing microbial inoculum of sewage and preparation method thereof |
| CN111748483A (en) * | 2019-03-29 | 2020-10-09 | 中国科学院沈阳应用生态研究所 | Bacillus for degrading petroleum hydrocarbon and application thereof |
| CN113265362A (en) * | 2021-06-23 | 2021-08-17 | 中南大学 | Composite microbial inoculum for efficiently converting soil heavy metal chromium as well as preparation method and application thereof |
| CN114480202A (en) * | 2022-02-16 | 2022-05-13 | 湖北茂盛生物有限公司 | Bioremediation microbial inoculum for petroleum-polluted soil, preparation device and method |
Non-Patent Citations (2)
| Title |
|---|
| 微生物对储油罐底油泥无公害处理初步研究;田永娥;《中国优秀硕士学位论文全文数据库》;全文 * |
| 石油降解菌的筛选及其降解性能研究;王龙;《中国优秀硕士学位论文全文数据库》;全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115305201A (en) | 2022-11-08 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Saeed et al. | Microbial bioremediation strategies with wastewater treatment potentialities–A review | |
| CN108821446B (en) | Preparation method of permeable reaction wall for relieving zero-valent iron passivation | |
| Guo et al. | Effective immobilization of Bacillus subtilis in chitosan-sodium alginate composite carrier for ammonia removal from anaerobically digested swine wastewater | |
| CN103276023B (en) | Method for promoting anaerobic fermentation of surplus sludge to produce acid by using biosurfactant | |
| CN104673710B (en) | Rhodococcus strain and its application | |
| CN105753270B (en) | A kind of water purification system suitable for high ammonia nitrogen livestock breeding wastewater | |
| CN105731750B (en) | A kind of method of mixed-matrix bioleaching removal sludge heavy-metal | |
| CN105347480A (en) | Method used for promoting biological reduction of nitrobenzene with biological carbon | |
| CN115305201B (en) | Microbial agent for efficiently treating oily sludge and preparation method thereof | |
| CN108971221B (en) | Method for restoring nitrobenzene contaminated soil | |
| Gül | Bioremediation of dyes in textile wastewater | |
| US11141764B2 (en) | Method for remediation of contaminated lands | |
| Sun et al. | Research progress of bio-slurry remediation technology for organic contaminated soil | |
| CN114084965B (en) | Oil refining wastewater biological treatment agent and use method thereof | |
| CN108660094A (en) | Thermophilic desulfurization ground bacillus TS-1 and its application | |
| CN103663716A (en) | Discharge treatment system for produced water of polymer flooding oilfield | |
| CN106623406A (en) | Waste water and acid soil combined remediation method | |
| CN107500407A (en) | A kind of method that sewage is handled using marine alga glycolipid biosurfactant | |
| JP7053173B2 (en) | Biodegradation treatment method for organic compounds | |
| CN107384829B (en) | Application of bacillus megatherium YJB3 in artificial wetland water PAEs pollution purification | |
| Deive et al. | Bioreactor development for the cultivation of extremophilic microorganisms | |
| Zhou et al. | Progress and prospect of bioremediation technology for petroleum and petrochemical contaminated sites | |
| TWI616409B (en) | Emulsive composition and method of preparing the same | |
| Dey et al. | Immobilized chromate reducing bacteria and their enzymes in bioremediation of hexavalent chromium | |
| CN103667098A (en) | Pseudomonas putida C2 for degrading unsymmetrical dimethylhydrazine and a method thereof for degrading unsymmetrical dimethylhydrazine |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |