CN115305201A - Microbial agent for efficiently treating oily sludge and preparation method thereof - Google Patents
Microbial agent for efficiently treating oily sludge and preparation method thereof Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F11/00—Treatment of sludge; Devices therefor
- C02F11/02—Biological treatment
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
Abstract
The invention belongs to the technical field of sludge treatment, and particularly relates to a microbial agent for efficiently treating oily sludge and a preparation method thereof. According to the invention, three different culture media are designed, the oily sludge is inoculated to the different culture media, and through screening culture, the bacterial liquid cultured by the culture medium A can increase the surface activity of the oily sludge, so that the oily sludge is better eluted, and the bacterial liquid cultured by the culture media B and C can effectively degrade the sludge by taking oil in the oily sludge as a carbon source. The three bacterial liquids are compounded to obtain the microbial agent, the oily sludge can be efficiently eluted within 4-7 days, the petroleum pollutants can be degraded and removed, the preparation process is simple, the culture conditions are controllable and easy to realize, and the microbial agent is suitable for industrial popularization.
Description
Technical Field
The invention belongs to the technical field of sludge treatment, and particularly relates to a microbial agent for efficiently treating oily sludge and a preparation method thereof.
Background
The petroleum industry has generated a great deal of oily sludge during the exploration, production, transportation, storage and refinement of crude oil, and the problem of treatment and disposal of the oily sludge has received increasing attention in recent years. Oily sludge, which contains high concentrations of Petroleum Hydrocarbons (PHCs) and other components that are difficult to degrade, is considered a hazardous waste in many countries, and improper disposal or insufficient disposal poses serious threats to the environment and human health, and its effective remediation has become a worldwide problem due to the increasing harmfulness and yield of oily sludge. Over the years, various oily sludge treatment methods have been developed, such as farming, incineration, solidification/stabilization, solvent extraction, ultrasonic treatment, pyrolysis, photocatalysis, chemical treatment, biodegradation, and the like. By using these techniques, the content of hazardous components can be reduced or eliminated, thereby mitigating their harmful effects on the environment and health.
The biological treatment utilizes the action of soil indigenous or exogenous microorganisms to remove pollutants, repairs petroleum polluted environment by accelerating the degradation of PHCs by microorganisms, is simple to operate, does not need to manage and generate secondary pollutants, can better accord with the strategy of sustainable development, and is concerned by numerous scholars in the field of environmental microorganisms. Therefore, the development of microbial agents for efficiently treating oily sludge is urgently needed.
Disclosure of Invention
In order to solve the technical problems, different culture media are adopted to screen and culture microbial agents in the oily sludge, so that microbial solutions capable of promoting the surface activity of the oily sludge and effectively degrading the oily sludge are respectively obtained, and the efficient degradation of the oily sludge is realized.
In order to achieve the above purpose, an embodiment of the present invention provides a preparation method of a microbial agent for efficiently treating oily sludge, the preparation method specifically includes the following steps:
respectively inoculating the oily sludge to a culture medium A, a culture medium B and a culture medium C for culture to obtain a bacterial liquid A, a bacterial liquid B and a bacterial liquid C;
and (3) mixing the bacterial liquid A, the bacterial liquid B and the bacterial liquid C according to the ratio of 0.1-10:0.1-10: mixing at a ratio of 0.1-10 to obtain a microbial agent;
the A culture medium is as follows: 1 to 10 portions of glucose, 0.1 to 10 portions of yeast extract, 1 to 10 portions of urea and K 2 HPO 4 ·3H 2 1 to 10 portions of O and KH 2 PO 4 1 to 10 portions of MgSO 4 ·7H 2 1-10 parts of O and 1-10 parts of trace element liquid; detachment1000 parts of sub-water;
the culture medium B comprises: naNO 3 1 to 15 portions of K 2 HPO 4 ·3H 2 1 to 10 portions of O and KH 2 PO 4 1 to 10 portions of MgSO 4 ·7H 2 0.1 to 1 portion of O, 1 to 10 portions of NaCl, 1 to 10 portions of KCl and CaCl 2 0.01 to 1 portion of FeCl 3 0.01 to 1 portion and 1000 portions of deionized water;
the culture medium C is as follows: NH 4 Cl 0.1-1 part, K 2 HPO 4 ·3H 2 1 to 10 portions of O and KH 2 PO 4 1 to 10 portions of MgSO 4 ·7H 2 0.1 to 1 portion of O, 1 to 10 portions of NaCl, 1 to 10 portions of KCl and CaCl 2 0.01 to 1 portion of FeCl 3 0.01 to 1 portion and 1000 portions of deionized water.
Further, the trace element liquid is: znSO 4 ·7H 2 0.1 to 10 portions of O and MnSO 4 ·H 2 0.1 to 10 portions of O and FeSO 4 ·7H 2 0.1 to 10 portions of O and CaCl 2 ·2H 2 0.01 to 1 portion of O and CoCl 2 0.01-1 part of 6H2O and CuSO 4 ·5H 2 0.01 to 1 portion of O and H 3 BO 3 0.01 to 1 portion of Na 2 MoO 4 0.01-1 part of H2O and 1000 parts of deionized water.
Further, the inoculation amount of the oily sludge is 1-5%.
Further, the process parameters of the culture process are as follows: the culture temperature is as follows: 25-35 ℃; rotating speed of a shaking table: 110-200r/min, culture time: 2-7 days.
Further, the pH value of the culture medium A is 7.0-7.5 after the culture medium A is prepared, and the culture medium A is sterilized by high-pressure steam for 30min at the temperature of 115 ℃; the pH value of the culture medium B and the culture medium C is 7.0-7.5 after the culture medium B and the culture medium C are configured, and the culture medium B and the culture medium C are sterilized by high-pressure steam for 20min at the temperature of 121 ℃.
Further, the concentration of the bacterial liquid A, the bacterial liquid B and the bacterial liquid C is more than or equal to 10 8 /mL。
Based on the same inventive concept, the embodiment of the invention also provides a microbial agent for efficiently treating oily sludge, and the microbial agent is prepared by the preparation method.
Has the advantages that:
according to the invention, different culture media are designed, the oily sludge is inoculated to different culture media, and through screening culture, the bacterial liquid cultured by the culture medium A can increase the surface activity of the oily sludge, so that the oily sludge is better eluted, and the bacterial liquid cultured by the culture media B and C can effectively degrade the sludge by taking oil in the oily sludge as a carbon source. The three bacterial liquids are compounded according to a certain proportion, so that the oily sludge can be efficiently eluted within 4-7 days, and petroleum pollutants can be degraded and removed. The microbial inoculum is simple in preparation process, controllable in culture conditions, easy to realize and suitable for industrial popularization.
Detailed Description
In order to more clearly illustrate the technical content of the present invention, the detailed description is given in conjunction with specific examples, it is obvious that the examples are only the preferred embodiments of the technical solution, and other technical solutions which can be obviously derived by those skilled in the art from the technical content disclosed still belong to the protection scope of the present invention.
In the embodiment of the present invention, the chemical reagents used can be prepared by the methods of purchase or existing preparation methods, and the equipment used is the conventional equipment in the prior art.
Example 1
Preparing a culture medium, namely weighing the following raw materials in parts by weight: 5 parts by weight of glucose, 1 part by weight of Yeast extract, 1.5 parts by weight of Urea, and K 2 HPO 4 ·3H 2 1.5 parts by weight of O, KH 2 PO 4 1.5 parts by weight of MgSO 2 4 ·7H 2 0.5 part of O, 1 part of NaCl, 1 part of KCl, 1 part of trace element liquid and 1000 parts of deionized water, stirring and dissolving, then performing pH 7.2, and performing high-pressure steam sterilization at 115 ℃ for 30min. Wherein the trace elements are prepared as follows: znSO 4 ·7H 2 0.1 part by weight of O, mnSO 4 ·H 2 0.25 part by weight of O, feSO 4 ·7H 2 0.1 part by weight of O and CaCl 2 ·2H 2 0.01 part by weight of O, coCl 2 0.1 part by weight of 6H2O, cuSO 4 ·5H 2 0.1 part by weight of O, H 3 BO 3 0.01 part by weight of Na 2 MoO 4 ·H 2 0.02 part by weight of O and 1000 parts by weight of deionized water.
And B, preparing a culture medium, namely weighing the following raw materials in parts by weight: NH (NH) 4 Cl 0.8 parts by weight, K 2 HPO 4 ·3H 2 O1 part by weight, KH 2 PO 4 1 part by weight of MgSO (MgSO) 4 ·7H 2 0.5 part of O, 1 part of NaCl, 1 part of KCl and CaCl 2 0.02 part by weight of FeCl 3 0.01 weight portion and 1000 weight portions of deionized water, stirring to dissolve, then the pH value is 7.2, and high-pressure steam sterilization is carried out for 20min at the temperature of 121 ℃.
C, preparing a culture medium, namely weighing the following raw materials in parts by weight: naNO 3 1.3 parts by weight of K 2 HPO 4 ·3H 2 O1 part by weight, KH 2 PO 4 1 part by weight of MgSO (MgSO) 4 ·7H 2 0.5 part of O, 1 part of NaCl, 1 part of KCl and CaCl 2 0.02 part by weight of FeCl 3 0.01 weight portion and 1000 weight portions of deionized water, stirring and dissolving, then pH is 7.2, and high-pressure steam sterilizing is carried out for 20min at the temperature of 121 ℃.
Collecting oil-containing sludge of Liaohe oil field as inoculum to prepare bacterial liquid suspension, inoculating the oil-containing sludge into three culture media A, B and C according to the inoculum concentration of 3%, culturing for 7 days at 30 ℃ in a shaking table at the rotating speed of 180r/min, and obtaining corresponding bacterial liquid when the bacterial liquid concentration in each culture medium reaches 108/mL, wherein the bacterial liquid A, the bacterial liquid B and the bacterial liquid C are obtained.
And mixing 1 part by weight of the bacterial liquid A, 1 part by weight of the bacterial liquid B and 1 part by weight of the bacterial liquid C to obtain the microbial agent.
An experiment is carried out on oily sludge (with the oil content of 5-20%) retrieved from a Liaohe oil field, 150g of the oily sludge is pretreated with water according to a certain proportion, and 5 experimental groups are set, namely a control group (without adding strains), a group A (with bacterial liquid A), a group B (with liquid B), a group C (with liquid C) and a group D (with the microbial agent obtained in example 1); the inoculation amounts of the group A, B, C and D inoculants are all 2%; wherein the oily sludge of A after 7 days is obviously yellow, the black viscosity is reduced, and the removal rate of the petroleum hydrocarbon pollutants is shown in Table 1.
TABLE 1
| Experimental group | Control group | Group A | Group B | Group C | Group D |
| Original oil content/g.kg -1 | 150.6 | 158.5 | 152.5 | 155.7 | 160.4 |
| Oil content/g.kg after treatment -1 | 145.3 | 105.1 | 81.5 | 89.5 | 63.3 |
| Removal rate% | 3.5 | 34.6 | 46.5 | 42.5 | 60.5 |
Example 2
Preparing a culture medium, namely weighing the following raw materials in parts by weight: 3 parts by weight of glucose, 0.6 part by weight of Yeast extract, 1.2 parts by weight of Urea, and K 2 HPO 4 ·3H 2 O2 part by weight, KH 2 PO 4 2 parts by weight of MgSO 4 ·7H 2 0.3 part of O, 2 parts of NaCl, 2 parts of KCl, 2 parts of trace element liquid and 1000 parts of deionized water, stirring and dissolving, then performing pH 7.4, and performing high-pressure steam sterilization at 115 ℃ for 30min. Wherein the trace elements are prepared as follows: znSO 4 ·7H 2 0.2 part by weight of O, mnSO 4 ·H 2 0.5 part by weight of O, feSO 4 ·7H 2 0.5 part of O and CaCl 2 ·2H 2 0.05 part by weight of O, coCl 2 0.5 parts by weight of 6H2O, cuSO 4 ·5H 2 0.5 part by weight of O, H 3 BO 3 0.03 part by weight of Na 2 MoO 4 ·H 2 0.05 part of O and 1000 parts of deionized water.
B, preparing a culture medium, namely weighing the following raw materials in parts by weight: NH (NH) 4 Cl 0.6 parts by weight, K 2 HPO 4 ·3H 2 O3 part by weight, KH 2 PO 4 2 parts by weight of MgSO 2 4 ·7H 2 0.8 part of O, 2 parts of NaCl, 2 parts of KCl and CaCl 2 0.06 parts by weight of FeCl 3 0.04 part by weight and 1000 parts by weight of deionized water, stirring and dissolving, then performing pH 7.4, and performing high-pressure steam sterilization at 121 ℃ for 20min.
C, preparing a culture medium, namely weighing the following raw materials in parts by weight: naNO 3 1.6 parts by weight of K 2 HPO 4 ·3H 2 O2 part by weight, KH 2 PO 4 2 parts by weight of MgSO 2 4 ·7H 2 O1, naCl 2, KCl 2 and CaCl 2 0.04 parts by weight of FeCl 3 0.03 weight part and 1000 weight parts of deionized water, stirring to dissolve, then carrying out pH 7.4, and carrying out high-pressure steam sterilization at 121 ℃ for 20min.
Collecting oil-containing sludge of Liaohe oilfield as inoculum to prepare bacterial liquid suspension, inoculating the oil-containing sludge into three culture media A, B and C according to inoculum concentration of 2%, culturing at 28 deg.C and rotation speed of 150r/min in shaking table for 7 days, and allowing the bacterial liquid concentration in each culture medium to reach 10 8 And obtaining corresponding bacterial liquid, namely bacterial liquid A, bacterial liquid B and bacterial liquid C by using the bacterium/mL.
And mixing 3 parts by weight of the bacterial liquid A, 2 parts by weight of the bacterial liquid B and 3 parts by weight of the bacterial liquid C to obtain the microbial agent.
Carrying out experiments on oily sludge (with the oil content of 5-20%) retrieved from Liaohe oil field, pretreating 150g of the oily sludge with water according to a certain proportion, and setting 5 experimental groups, namely a control group (without adding strains), a group A (with bacterial liquid A added), a group B (with liquid B added), a group C (with liquid C added) and a group D (with the microbial agent obtained in example 2); the inoculation amounts of the group A, B, C and D bactericides are all 2 percent; after 7 days, the oily sludge turns yellow obviously, the black color is less viscous, and the removal rate of the petroleum hydrocarbon pollutants is shown in Table 2.
TABLE 2
| Experimental group | Control group | Group A | Group B | Group C | Group D |
| Original oil content/g.kg -1 | 155.6 | 157.6 | 153.5 | 157.6 | 162.1 |
| Oil content/g.kg after treatment -1 | 151.3 | 104.9 | 83.7 | 89.1 | 59.3 |
| Removal rate% | 2.8 | 33.4 | 45.5 | 43.5 | 63.4 |
Example 3
Preparing a culture medium, namely weighing the following raw materials in parts by weight: glucose 7 parts, yeast extract 5 parts, urea 3 parts, K 2 HPO 4 ·3H 2 O2.5 parts by weight, KH 2 PO 4 2.5 parts by weight of MgSO 2 4 ·7H 2 0.5 part of O, 2.5 parts of NaCl, 2.5 parts of KCl, 2.5 parts of trace element liquid and 1000 parts of deionized water, stirring and dissolving, then, the pH value is 7.2, and the mixture is sterilized by high-pressure steam for 30min at the temperature of 115 ℃. Wherein the trace elements are prepared as follows: znSO 4 ·7H 2 0.4 part by weight of O, mnSO 4 ·H 2 0.7 part by weight of O, feSO 4 ·7H 2 0.7 part of O and CaCl 2 ·2H 2 O 0.07 parts by weight of CoCl 2 0.7 part by weight of 6H2O, cuSO 4 ·5H 2 0.7 part by weight of O, H 3 BO 3 0.05 part by weight of Na 2 MoO 4 ·H 2 0.07 part of O and 1000 parts of deionized water.
And B, preparing a culture medium, namely weighing the following raw materials in parts by weight: NH (NH) 4 Cl 0.75 parts by weight, K 2 HPO 4 ·3H 2 O5 parts by weight, KH 2 PO 4 3 parts by weight of MgSO 4 ·7H 2 0.5 weight portion of O, 3.5 weight portions of NaCl, 3.5 weight portions of KCl and CaCl 2 0.12 parts by weight of FeCl 3 0.08 weight part and 1000 weight parts of deionized water, stirring and dissolving, then performing pH 7.2, and performing high-pressure steam sterilization at 121 ℃ for 20min.
C, preparing a culture medium, namely weighing the following raw materials in parts by weight: naNO 3 2.4 parts by weight of K 2 HPO 4 ·3H 2 O3.5 parts by weight KH 2 PO 4 3.5 parts by weight of MgSO 2 4 ·7H 2 0.7 weight portion of O, 3.5 weight portions of NaCl, 3.5 weight portions of KCl and CaCl 2 0.12 parts by weight of FeCl 3 0.14 weight portion and 1000 weight portions of deionized water, stirring and dissolving, then the pH value is 7.2, and sterilizing for 20min by high-pressure steam under the condition of 121 ℃.
Collecting oil-containing sludge of Liaohe oilfield as inoculum to prepare bacterial liquid suspension, inoculating 5% of the oil-containing sludge into three culture media A, B and C, culturing at 33 deg.C at 190r/min in shaking table for 6 days, and allowing bacterial liquid concentration in each culture medium to reach 10 8 and/mL can be used for obtaining corresponding bacterial liquid, namely bacterial liquid A, bacterial liquid B and bacterial liquid C.
And mixing 5 parts by weight of the bacterial liquid A, 4 parts by weight of the bacterial liquid B and 5 parts by weight of the bacterial liquid C to obtain the microbial agent.
An experiment is carried out on oily sludge (with the oil content of 5-20%) retrieved from a Liaohe oil field, 150g of the oily sludge is pretreated with water according to a certain proportion, and 5 experimental groups are set, namely a control group (without adding strains), a group A (with bacterial liquid A), a group B (with liquid B), a group C (with liquid C) and a group D (with the microbial agent obtained in example 3); the inoculation amounts of the group A, B, C and D bactericides are all 2 percent; the removal rate of the petroleum hydrocarbon contaminants after 6 days is shown in Table 3.
TABLE 3
Experiments show that the removal rate of petroleum pollutants in the oily sludge by singly inoculating the three bacterial agents A, B and C is less than 50%, and the removal rate of pollutants in the oily sludge in an experimental group inoculated with the mixed bacterial liquid added in proportion is much higher than that of other experimental groups. After the subsequent physical and chemical treatment, the oil content of the bottom mud in the oily sludge is less than 1 percent, and the COD in the supernatant is less than 9, so that the harmless treatment of the oily sludge is realized.
The above-mentioned embodiments are only preferred embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered as the technical scope of the present invention, and equivalent substitutions or changes according to the technical solution of the present invention and its conception should be covered by the scope of the present invention.
Claims (7)
1. The preparation method of the microbial agent for efficiently treating the oily sludge is characterized by comprising the following steps of:
respectively inoculating the oily sludge to a culture medium A, a culture medium B and a culture medium C for culture to obtain a bacterial liquid A, a bacterial liquid B and a bacterial liquid C;
and (3) mixing the bacterial liquid A, the bacterial liquid B and the bacterial liquid C according to the ratio of 0.1-10:0.1-10: mixing at a ratio of 0.1-10 to obtain a microbial agent;
the A culture medium is as follows: 1 to 10 portions of glucose, 0.1 to 10 portions of yeast extract, 1 to 10 portions of urea and K 2 HPO 4 ·3H 2 1 to 10 portions of O and KH 2 PO 4 1 to 10 portions of MgSO 4 ·7H 2 1 to 10 portions of O and trace elements1-10 parts of a vegetable liquid; 1000 parts of deionized water;
the culture medium B comprises: naNO 3 1 to 15 portions of K 2 HPO 4 ·3H 2 1 to 10 portions of O and KH 2 PO 4 1 to 10 portions of MgSO 4 ·7H 2 0.1 to 1 portion of O, 1 to 10 portions of NaCl, 1 to 10 portions of KCl and CaCl 2 0.01 to 1 portion of FeCl 3 0.01 to 1 portion and 1000 portions of deionized water;
the culture medium C comprises: NH 4 0.1 to 1 portion of Cl and K 2 HPO 4 ·3H 2 1 to 10 portions of O and KH 2 PO 4 1 to 10 portions of MgSO 4 ·7H 2 0.1 to 1 portion of O, 1 to 10 portions of NaCl, 1 to 10 portions of KCl and CaCl 2 0.01 to 1 portion of FeCl 3 0.01 to 1 portion and 1000 portions of deionized water.
2. The method for preparing the microbial agent for efficiently treating the oily sludge according to claim 1, wherein the trace element liquid is: znSO 4 ·7H 2 0.1 to 10 portions of O and MnSO 4 ·H 2 0.1 to 10 portions of O and FeSO 4 ·7H 2 0.1 to 10 portions of O and CaCl 2 ·2H 2 0.01 to 1 portion of O and CoCl 2 0.01-1 part of 6H2O and CuSO 4 ·5H 2 0.01 to 1 portion of O and H 3 BO 3 0.01 to 1 portion of Na 2 MoO 4 0.01-1 part of H2O and 1000 parts of deionized water.
3. The method for preparing a microbial agent for efficiently treating oily sludge according to claim 1, wherein the inoculation amount of the oily sludge is 1-5%.
4. The preparation method of the microbial agent for efficiently treating oily sludge according to claim 1, wherein the culture process comprises the following process parameters:
the culture temperature is as follows: 25-35 ℃; rotating speed of a shaking table: 110-200r/min, culture time: 2-7 days.
5. The method for preparing the microbial agent for efficiently treating the oily sludge according to claim 1, wherein the culture medium A is configured to have a pH value of 7.0-7.5 and is autoclaved at 115 ℃ for 30min; the pH value of the culture medium B and the culture medium C is 7.0-7.5 after the culture medium B and the culture medium C are configured, and the culture medium B and the culture medium C are sterilized by high-pressure steam for 20min at the temperature of 121 ℃.
6. The method for preparing the microbial agent for efficiently treating oily sludge according to claim 1, wherein the concentration of the bacterial liquid A, the bacterial liquid B and the bacterial liquid C is not less than 10 8 /mL。
7. A microbial agent for efficiently treating oily sludge, which is prepared by the preparation method of any one of claims 1 to 6.
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Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690939A (en) * | 2009-07-13 | 2010-04-07 | 延安市微生物研究所 | Method for degrading crude oil by separating compound bacteria solution from contaminated fossil oil sample |
| CN103865855A (en) * | 2014-03-25 | 2014-06-18 | 南京工业大学 | Bacillus subtilis strain and applications thereof |
| CN104388312A (en) * | 2014-12-10 | 2015-03-04 | 青岛农业大学 | Screening method for petroleum degrading bacteria, method for preparing petroleum degrading bacteria inoculant from screened bacteria, and application of inoculant |
| CN104450597A (en) * | 2014-12-10 | 2015-03-25 | 青岛农业大学 | Preparation method of petroleum degrading bacteria solid inoculant and method for restoring petroleum-polluted soil by using prepared solid inoculant |
| CN106929419A (en) * | 2017-04-12 | 2017-07-07 | 临沂大学 | A kind of screening technique of anaerobic degradation petroleum hydrocarbon methanogen floras |
| CN106929420A (en) * | 2017-04-12 | 2017-07-07 | 临沂大学 | A kind of method of decomposing petroleum hydrocarbon |
| CN107201311A (en) * | 2017-04-12 | 2017-09-26 | 临沂大学 | A kind of screening technique of anaerobic degradation petroleum hydrocarbon methanogen floras |
| CN107338206A (en) * | 2017-07-24 | 2017-11-10 | 青岛金典生化器材有限公司 | A kind of culture medium for being enriched with Bacillus acidi lactici and preparation method thereof |
| CN109161499A (en) * | 2018-08-29 | 2019-01-08 | 华南理工大学 | A kind of application for producing surfactant bacterium and its cutting down polycyclic aromatic hydrocarbon in situ in coal/petrochemical wastewater |
| CN109401974A (en) * | 2018-12-05 | 2019-03-01 | 河北省地质环境监测院 | A kind of oil-polluted soils microbial treatment method |
| CN109777759A (en) * | 2019-03-20 | 2019-05-21 | 天津瑞达同方生物科技开发有限公司 | It is a kind of for oily sludge and the processing microbial inoculum of sewage and preparation method thereof |
| CN111748483A (en) * | 2019-03-29 | 2020-10-09 | 中国科学院沈阳应用生态研究所 | Bacillus for degrading petroleum hydrocarbon and application thereof |
| CN113265362A (en) * | 2021-06-23 | 2021-08-17 | 中南大学 | Composite microbial inoculum for efficiently converting soil heavy metal chromium as well as preparation method and application thereof |
| CN114480202A (en) * | 2022-02-16 | 2022-05-13 | 湖北茂盛生物有限公司 | Bioremediation microbial inoculum for petroleum-polluted soil, preparation device and method |
-
2022
- 2022-09-16 CN CN202211126956.7A patent/CN115305201B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101690939A (en) * | 2009-07-13 | 2010-04-07 | 延安市微生物研究所 | Method for degrading crude oil by separating compound bacteria solution from contaminated fossil oil sample |
| CN103865855A (en) * | 2014-03-25 | 2014-06-18 | 南京工业大学 | Bacillus subtilis strain and applications thereof |
| CN104388312A (en) * | 2014-12-10 | 2015-03-04 | 青岛农业大学 | Screening method for petroleum degrading bacteria, method for preparing petroleum degrading bacteria inoculant from screened bacteria, and application of inoculant |
| CN104450597A (en) * | 2014-12-10 | 2015-03-25 | 青岛农业大学 | Preparation method of petroleum degrading bacteria solid inoculant and method for restoring petroleum-polluted soil by using prepared solid inoculant |
| CN106929419A (en) * | 2017-04-12 | 2017-07-07 | 临沂大学 | A kind of screening technique of anaerobic degradation petroleum hydrocarbon methanogen floras |
| CN106929420A (en) * | 2017-04-12 | 2017-07-07 | 临沂大学 | A kind of method of decomposing petroleum hydrocarbon |
| CN107201311A (en) * | 2017-04-12 | 2017-09-26 | 临沂大学 | A kind of screening technique of anaerobic degradation petroleum hydrocarbon methanogen floras |
| CN107338206A (en) * | 2017-07-24 | 2017-11-10 | 青岛金典生化器材有限公司 | A kind of culture medium for being enriched with Bacillus acidi lactici and preparation method thereof |
| CN109161499A (en) * | 2018-08-29 | 2019-01-08 | 华南理工大学 | A kind of application for producing surfactant bacterium and its cutting down polycyclic aromatic hydrocarbon in situ in coal/petrochemical wastewater |
| CN109401974A (en) * | 2018-12-05 | 2019-03-01 | 河北省地质环境监测院 | A kind of oil-polluted soils microbial treatment method |
| CN109777759A (en) * | 2019-03-20 | 2019-05-21 | 天津瑞达同方生物科技开发有限公司 | It is a kind of for oily sludge and the processing microbial inoculum of sewage and preparation method thereof |
| CN111748483A (en) * | 2019-03-29 | 2020-10-09 | 中国科学院沈阳应用生态研究所 | Bacillus for degrading petroleum hydrocarbon and application thereof |
| CN113265362A (en) * | 2021-06-23 | 2021-08-17 | 中南大学 | Composite microbial inoculum for efficiently converting soil heavy metal chromium as well as preparation method and application thereof |
| CN114480202A (en) * | 2022-02-16 | 2022-05-13 | 湖北茂盛生物有限公司 | Bioremediation microbial inoculum for petroleum-polluted soil, preparation device and method |
Non-Patent Citations (2)
| Title |
|---|
| 王龙: "石油降解菌的筛选及其降解性能研究", 《中国优秀硕士学位论文全文数据库》 * |
| 田永娥: "微生物对储油罐底油泥无公害处理初步研究", 《中国优秀硕士学位论文全文数据库》 * |
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