CN115287174A - Double-temperature-zone test tube temperature adjusting device and fluorescence detection device with same - Google Patents
Double-temperature-zone test tube temperature adjusting device and fluorescence detection device with same Download PDFInfo
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- CN115287174A CN115287174A CN202211017766.1A CN202211017766A CN115287174A CN 115287174 A CN115287174 A CN 115287174A CN 202211017766 A CN202211017766 A CN 202211017766A CN 115287174 A CN115287174 A CN 115287174A
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
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- B01L7/00—Heating or cooling apparatus; Heat insulating devices
- B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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Abstract
The invention discloses dual-temperature-zone test tube temperature adjusting equipment which comprises two temperature adjusting areas with different temperatures, wherein the two temperature adjusting areas are connected through a sliding rail, and a movable base used for placing a plurality of test tubes and exposing the bottoms of the test tubes is arranged on the sliding rail in a sliding manner; all be provided with the temperature regulation base that can be in vertical direction up-and-down motion by the elevating gear drive in every temperature regulation district, the base that adjusts the temperature sets up in moving the base below, and a plurality of temperature regulation grooves are seted up at the base top that adjusts the temperature, and the quantity and the position of temperature regulation groove and the test tube one-to-one in the moving base. The invention also provides fluorescence detection equipment, wherein the light path detection device is arranged between the two temperature adjusting areas to perform fluorescence detection on the test tube. The invention has simple structure, but utilizes the wrapped temperature regulation, the temperature regulation efficiency is high, and the temperature control is more accurate; the two temperature adjusting areas are adjusted to the specified temperature at the beginning, and in the whole temperature adjusting process, the temperature does not need to be raised and lowered in each circulation, so that the experiment efficiency is greatly improved.
Description
Technical Field
The invention relates to the field of biological detection, in particular to double-temperature-zone test tube temperature adjusting equipment and fluorescence detection equipment with the same.
Background
The PCR technique, polymerase Chain Reaction (PCR), was established by Kary Mullis of PE Cetus, USA, in 1983 (1993, nobel prize). The technology can amplify specific DNA fragments by millions of times after reaction for hours in a test tube, and the technology for rapidly obtaining a large number of single nucleic acid fragments has great significance in molecular biology research, thereby greatly promoting the research progress of life science.
PCR consists of three basic reaction steps of denaturation, annealing and extension, after the steps of denaturation, annealing and extension of DNA, fluorescence value detection is carried out on a test tube through a light path detection device, detection work after one cycle of PCR is realized, one fluorescence value is measured after each cycle, and after multiple times of measurement, a growth curve is drawn by the fluorescence values, so that the growth curve can be used for observing the growth of the quantity of the DNA.
The temperature adjusting modes of the rapid fluorescence quantitative detection equipment in the prior art are mainly two, one mode is that a test tube is placed in a cavity, and the cavity is heated by cold air and hot air so as to heat the test tube, so that the temperature rise speed is high, the space temperature difference is small, but the temperature control is not accurate enough, the cost is high, and the compatibility of 96-hole amplification tubes cannot be realized; another way is to use the semiconductor to carry out temperature regulation to the module, and this kind of mode accuse temperature is accurate, but rate of heating is slower, and the radiating rate is also slower simultaneously, therefore overall efficiency is lower.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide a dual-temperature-zone test tube temperature regulating device which has the advantages of convenience in use, high temperature rising and falling speed and accurate temperature control, and the technical purpose is realized through the following technical scheme:
a dual-temperature-zone test tube temperature adjusting device comprises two temperature adjusting areas with different temperature adjusting temperatures, wherein the two temperature adjusting areas are connected through a slide rail, the slide rail is led into the two temperature adjusting areas, and a movable base used for placing a plurality of test tubes and exposing the bottoms of the test tubes is arranged on the slide rail in a sliding manner;
the temperature adjusting base is arranged in each temperature adjusting area and driven by the lifting device to move up and down in the vertical direction, the temperature adjusting base is arranged below the movable base, a plurality of temperature adjusting grooves are formed in the top of the temperature adjusting base, and the number and the positions of the temperature adjusting grooves correspond to the test tubes in the movable base one by one.
Owing to set up the temperature adjustment base of liftable, the base that adjusts the temperature rises and makes the test tube arrange the tempering tank in, heats the test tube, and the test tube descends after the heating is accomplished, realizes adjusting the temperature to the test tube fast.
Further: the size and the shape of the inner cavity of the temperature adjusting groove are matched with the bottom of the test tube.
So set up, the tempering tank just wraps up at the test tube outer wall, does not have the clearance basically between test tube and the tempering tank, makes temperature regulation efficiency promote by a wide margin.
Further: the base adjusts the temperature for the metal material, adjusts the temperature the base and provides the heat by heating element.
So set up, usable heating element wholly heaies up the base that adjusts the temperature of metal, and it is more even to heat on the one hand, makes the test tube obtain adjusting the temperature of full aspect, improves the efficiency that adjusts the temperature, and on the other hand can heat the base that adjusts the temperature well in advance, saves preheating time.
Further: the heating element is an electric heating film.
The electric heating film is adopted to heat the temperature-adjusting base, so that the environment is protected and the energy is saved.
Further: the temperature adjusting base is made of copper or aluminum.
The copper and the aluminum have good heat-conducting property, and the temperature-adjusting base made of copper and aluminum is the preferred material after multiple tests and consideration of factors such as economy and the like.
Further: the lifting device comprises
Fixing a bracket;
installing a support: the fixing bracket is fixedly arranged on the mounting bracket and is used for limiting the mounting box body in the horizontal direction;
a cam: the driving mechanism is arranged on the fixed bracket or the mounting bracket and is driven by the driving mechanism to rotate along the horizontal rotating shaft;
buffering the top block: the cam is horizontally arranged above the cam and ascends or descends along with the rotation of the cam;
installing a box body: the temperature adjusting base is arranged in the mounting box body;
a guide column: the guide post upper end set firmly in the installation box body, the outside of guide post is located to buffering kicking block sliding sleeve, and the guide post bottom is equipped with the spacing flange of butt in buffering kicking block bottom.
Through setting up eccentric cam, by actuating mechanism drive cam such as motor and rotate, the cam rotation drives installation box body and the base that adjusts the temperature and rises, carries out rapid heating to the test tube, and the cam rotates half a circle again, and the base that adjusts the temperature falls to the minimum, stops adjusting the temperature, later removes the base and removes another district that adjusts the temperature and repeat above-mentioned process that adjusts the temperature.
Further, the method comprises the following steps: the outside cover of guide post is equipped with the spring, the spring bottom set up in on the buffering kicking block, the top butt of spring when the guide post rises with the bottom of installation box body.
The spring is sleeved outside the guide post, when the buffering top block moves upwards, the spring is pre-pressed to enable the temperature adjusting base to be close to the test tube upwards, and the buffering top block further rises, so that seamless fit of the temperature adjusting groove and the test tube is guaranteed, and the temperature adjusting effect is better; simultaneously owing to set up the spring, the precision requirement just reduced to the rising of buffering kicking block, can effectively prevent to adjust the temperature the base upwards displacement too much and lead to the damage of test tube.
Further: the bottom of the installation box body is provided with an accommodating groove for accommodating the spring, and when the buffering top block rises, the top of the spring abuts against the top of the accommodating groove.
The accommodating groove has a certain transverse limiting effect on the spring, and the working performance of the spring is guaranteed.
Further: the guide post is characterized in that a threaded hole extending downwards is formed in the accommodating groove, and a threaded section is arranged at the upper part of the guide post and is matched with the threaded hole.
The guide post and the mounting base are connected through threads, so that the structure is simple, and the disassembly is convenient; when the buffering ejector block rises, the spring can be sleeved outside the threaded hole to limit the inside of the spring, and the spring and the accommodating groove limit the spring inside and outside to ensure the working performance of the spring.
The invention also provides fluorescence detection equipment which comprises the dual-temperature-zone test tube temperature adjusting equipment and a light path detection device, wherein the light path detection device is arranged towards the movement path of the movable base between the two temperature adjusting areas.
Through set up light path detection device between two temperature regulation regions, light path detection device is prior art, and it has utilized the different characteristic of material reflection wavelength that should, carries out fluorescence quantitative determination to the test tube, and the temperature regulation that the test tube every passes through two temperature regulation regions is a circulation, carries out fluorescence quantitative determination to the test tube when it passes through between two temperature regulation regions, records data, draws the curve graph with a plurality of data record, can reflect the quantity change of DNA.
Compared with the prior art, the heating mode structure is not complicated, but the wrapped temperature regulation is utilized, the temperature regulation efficiency is far more efficient than that of a semiconductor or wind-heated mode, the temperature control is more accurate than that of a wind-heated mode, and the temperature error of a test tube can be basically guaranteed to be within 0.5 ℃;
on the whole, the two temperature adjusting areas are adjusted to the specified temperature at the beginning, and in the whole temperature adjusting process, the two temperature adjusting areas basically keep temperature adjustment without temperature rise and temperature reduction in each circulation, so that the experiment efficiency is greatly improved.
Drawings
FIG. 1 is a schematic view of the construction of an outer case of the present invention;
FIG. 2 is a schematic view of the overall structure of the present invention without the housing;
FIG. 3 is a schematic view of another angle overall configuration of the present invention without the housing;
FIG. 4 is a schematic structural view of the reaction lifting device driving the heating box body to lift;
FIG. 5 is a schematic view of the bottom of the mounting cartridge;
FIG. 6 is a schematic view of a heating chamber being lifted by a lifting device without a mounting box.
Fig. 7 is a schematic view of the connection relationship between the mounting frame and the mounting box body.
In the figure, 1, a temperature adjustment area; 2. a slide rail; 3. moving the base; 4. a temperature adjusting base; 41. a temperature adjusting tank; 5. a test tube; 6. fixing a bracket; 7. a cam; 8. buffering the top block; 9. a guide post; 91. a threaded segment; 92. a limiting flange; 10. installing a box body; 101. a containing groove; 102. a threaded hole; 103. a chute; 11. an optical path detection device; 12. a fan; 13. a box body; 14. a spring; 15. a mounting frame; 151. a slide bar.
Detailed Description
In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.
In the description of the present invention, it should be noted that, unless explicitly stated or limited otherwise, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, such as "connected," which may be fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meanings of the above terms in the present invention can be understood in specific cases to those skilled in the art.
Referring to fig. 1-3, a dual-temperature-zone test tube temperature adjustment device includes an external box 13, the box 13 mainly prevents external light from interfering with the optical path detection device 11, and also makes the integration of the whole device higher, and a fan 12 for heat dissipation is provided in the box.
Two temperature adjusting areas 1 are arranged in the box body 13, the temperature adjusted by the two temperature adjusting areas 1 is different, one of the two temperature adjusting areas is about 55-65 ℃, and the other temperature adjusting area is about 95 ℃. Be equipped with fixed bolster 6 in the box 13, two district 1 that adjust the temperature set up in the both sides of support 6, are provided with slide rail 2 on the support 6, and the sliding is provided with on slide rail 2 and removes base 3, removes base 3 and is used for installing test tube 5, and exposes test tube 5 bottom, is convenient for adjust the temperature. The slide rail 2 is arranged between the two temperature adjusting areas 1 and extends into the temperature adjusting areas 1. The structure of the driving belt is driven by the motor to drive the movable seat 3 to move. Every is provided with elevating gear and the base 4 that adjusts the temperature in the region, adjusts the temperature the base top be a plurality of with remove the test tube 5 one-to-one in the base 3 the adjustment tank 41, the shape of adjustment tank 41 inner chamber and the adaptation of test tube 5 bottom (if 5 bottoms of test tube are conical in this embodiment, 41 inner chambers of adjustment tank are also conical), guarantee that the adjustment tank laminates in the outside of test tube 5, make heating efficiency higher.
As shown in fig. 4-7, the lifting device is provided with a fixing support 6 in the box 13, a mounting support 15 is fixedly arranged on the fixing support 6, a sliding strip 151 is arranged on the mounting support 15, a sliding groove 103 is arranged on the mounting box body 10, and the mounting support 15 has a transverse limiting effect on the mounting box body 10 to prevent the mounting box body 10 from being separated from the mounting support, so that the mounting box body 10 vertically slides on the mounting support 15. The installation motor and by the rotatory eccentric settings's of motor drive cam 7 on the installing support 15, cam 7 top sets up buffering kicking block 8, and installation box body 10 is located buffering kicking block 8 top, and installation box body 10 top is used for the installation base 4 that adjusts the temperature, and storage tank 101 is seted up to the bottom, is equipped with downwardly extending's screw hole 102 in the storage tank for cooperate with screw thread section 91 on guide post 9 upper portion. The guide post 9 passes through the lower buffering ejector block 8, and a limiting flange 92 is arranged at the bottom of the guide post 9 and used for abutting against the bottom of the buffering ejector block 8. The guide post 9 is sleeved with a spring 14, the bottom of the spring 14 abuts against the buffering top block 8, when the buffering top block 8 ascends, the spring 14 ascends along with the buffering top block and is sleeved outside the threaded hole 102, and the top of the spring can abut against the top of the inverted accommodating groove 101. The spring 14 is sleeved outside the guide post 9, when the buffering top block 8 moves upwards, the spring 14 is pre-pressed to enable the temperature adjusting base 4 to be close to the test tube 5 upwards, and the buffering top block 8 further rises, so that seamless fit of the temperature adjusting groove 41 and the test tube 5 is guaranteed, and the temperature adjusting effect is better; simultaneously owing to set up spring 14, the precision requirement just has reduced to the rising of buffering kicking block 8, can effectively prevent to adjust temperature base 4 upwards displacement and too much lead to the damage of test tube 5.
When the buffering jacking block 8 moves upwards, the bottom of the temperature adjusting base 4 is jacked up to enable the temperature adjusting groove 41 to rise, the test tube 5 enters the temperature adjusting groove 41 to adjust the temperature, when the bottom cam 7 rotates for a half circle again, the temperature adjusting base 4 descends, the test tube 5 is separated from the temperature adjusting groove 41 to stop adjusting the temperature, and the movable base 3 continues to slide on the sliding rail to the temperature adjusting area 1 on the other side to adjust the temperature.
The temperature adjusting base 4 is preferably made of metal materials such as aluminum and copper, the temperature adjusting base 4 is heated by a heating device such as an electrothermal film, the temperature of the temperature adjusting base 4 is increased in all directions, the temperature can be preheated to a specified temperature in advance, the experiment time is saved, the temperature adjustment is more uniform, the efficiency is higher, and the basic temperature adjusting speed can be saved by 5-10 seconds compared with the prior art.
The invention also discloses a fluorescence detection device, as shown in fig. 2, a light path detection device 11 is arranged on the side surface of the path between the two temperature adjusting areas 1 of the movable seat 3, the light path detection device 11 is the prior art, the fluorescence in the test tube 5 is detected mainly by utilizing different reflection wavelengths of different colors, and after a plurality of cycles, the detection result of the light path detection device 11 is made into a curve chart for analyzing the quantity change of the DNA.
The whole working process is as follows: when a sample is placed in the movable seat for DNA denaturation, the movable seat 3 enters the right 95 ℃ temperature adjusting area 1 and is heated for a certain time until the reagent reaches 95 ℃ of the area, and the temperature rising speed depends on the heat conduction performance and the initial temperature of the reagent and the reagent tube; then the movable seat 3 enters a left 65 ℃ temperature adjusting area after passing through a middle normal temperature area in the way, so that a sample can be cooled to 65 ℃ from 95 ℃ as fast as possible, annealing and extension of DNA are carried out in the area, a cycle is completed, then the movable seat 3 moves to the 95 ℃ temperature adjusting area again, the light path detection device carries out quantitative detection and recording on the fluorescent substance in the moving process, and the processes are repeated.
The foregoing shows and describes the general principles and broad features of the present invention and advantages thereof. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (10)
1. The utility model provides a dual temperature district test tube attemperator, includes two temperature regulation areas (1) that the temperature that adjust the temperature is different, its characterized in that: the two temperature adjusting areas (1) are connected through a sliding rail (2), the sliding rail (2) leads into the two temperature adjusting areas (1), and a moving base (3) used for placing a plurality of test tubes (5) and enabling the bottoms of the test tubes (5) to be exposed is arranged on the sliding rail (2) in a sliding mode;
all be provided with in every temperature regulation region (1) by elevating gear drive can be in the temperature regulation base (4) of vertical direction up-and-down motion, temperature regulation base (4) set up in removal base (3) below, and a plurality of temperature regulation grooves (41) are seted up at temperature regulation base (4) top, the quantity and the position of temperature regulation groove (41) with test tube (5) one-to-one in the removal base (3).
2. The dual temperature zone test tube tempering device of claim 1, wherein: the size and the shape of the inner cavity of the temperature adjusting groove (41) are matched with the bottom of the test tube (5).
3. The dual temperature zone test tube tempering device of claim 1, wherein: the temperature adjusting base (4) is made of metal, and the temperature adjusting base (4) provides heat by a heating element.
4. The dual temperature zone test tube tempering device of claim 3, wherein: the heating element is an electric heating film.
5. The dual temperature zone test tube tempering device of claim 3, wherein: the temperature adjusting base (4) is made of copper or aluminum.
6. The dual temperature zone test tube tempering device of claim 1, wherein: the lifting device comprises
A fixed bracket (6);
mounting bracket (15): the fixing support is fixedly arranged on the fixing support (6) and used for limiting the installation box body (10) in the horizontal direction;
cam (7): the driving mechanism is arranged on the fixed bracket (6) or the mounting bracket (15) and is driven by the driving mechanism to rotate along the horizontal rotating shaft;
buffer top block (8): the horizontal device is arranged above the cam (7) and ascends or descends along with the rotation of the cam (7);
mounting case (10): the temperature adjusting base (4) is arranged on the side wall of the mounting support (15) in a sliding mode and is positioned above the buffering top block (8), and the temperature adjusting base (4) is arranged in the mounting box body (10);
guide post (9): guide post (9) upper end set firmly in installation box body (10), the outside of guide post (9) is located to buffering kicking block (8) slip cap, and guide post (9) bottom is equipped with spacing flange (92) of butt in buffering kicking block (8) bottom.
7. The dual temperature zone test tube tempering device of claim 6, wherein: the outside cover of guide post (9) is equipped with spring (14), spring (14) bottom set up in on buffering kicking block (8), when guide post (9) rise the top butt of spring (14) with the bottom of installation box body (10).
8. The dual temperature zone test tube tempering device of claim 7, wherein: the bottom of the mounting box body (10) is provided with a containing groove (101) for containing the spring (14), and when the buffering top block (8) rises, the top of the spring (14) is abutted to the top of the containing groove (101).
9. The dual temperature zone test tube tempering device of claim 8, wherein: a threaded hole (102) extending downwards is formed in the accommodating groove (101), and a threaded section (91) is arranged at the upper part of the guide post (9) and is matched with the threaded hole (102).
10. A fluorescence detection device, characterized by: double-temperature-zone test tube temperature regulating device comprising the double-temperature-zone test tube temperature regulating device according to any one of claims 1 to 9, further comprising a light path detection device (11), wherein the light path detection device (11) is arranged towards a movement path of the mobile base (3) between the two temperature regulating areas (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211017766.1A CN115287174A (en) | 2022-08-24 | 2022-08-24 | Double-temperature-zone test tube temperature adjusting device and fluorescence detection device with same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211017766.1A CN115287174A (en) | 2022-08-24 | 2022-08-24 | Double-temperature-zone test tube temperature adjusting device and fluorescence detection device with same |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN115287174A true CN115287174A (en) | 2022-11-04 |
Family
ID=83831535
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211017766.1A Pending CN115287174A (en) | 2022-08-24 | 2022-08-24 | Double-temperature-zone test tube temperature adjusting device and fluorescence detection device with same |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115287174A (en) |
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2022
- 2022-08-24 CN CN202211017766.1A patent/CN115287174A/en active Pending
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