CN215250871U - PCR detection system - Google Patents
PCR detection system Download PDFInfo
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- CN215250871U CN215250871U CN202120473386.3U CN202120473386U CN215250871U CN 215250871 U CN215250871 U CN 215250871U CN 202120473386 U CN202120473386 U CN 202120473386U CN 215250871 U CN215250871 U CN 215250871U
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Abstract
The utility model discloses a PCR detection system, which comprises a frame, and a temperature control device, an optical detection device, a chip fixing device and a control device which are arranged on the frame; the control device is respectively connected with the temperature control device, the optical detection device and the chip fixing device; the temperature control device is positioned below the chip fixing device; the optical detection device is positioned above the chip fixing device and is used for detecting the condition of the amplified nucleic acid in the reaction cavity of the chip; the control device is positioned above the optical detection device and used for driving the liquid in the chip to flow so as to realize the reaction and detection processes. The device simple structure, safety, effective, reliable and easy and simple to handle can avoid manual operation's error to cause the bad consequence that the error of result arouses.
Description
Technical Field
The utility model relates to a check out test set technical field especially relates to a PCR detecting system.
Background
Polymerase Chain Reaction (PCR) is a method of using a DNA fragment as a template, and amplifying the DNA fragment to a sufficient amount for structural and functional analysis in the presence of DNA polymerase and nucleotide substrates. The PCR detection method has very important significance in clinically and rapidly diagnosing bacterial infectious diseases and the like.
The principle of PCR is used to amplify a DNA fragment located between two known sequences, similar to the process of replication of natural DNA. The DNA molecule to be amplified is used as a template, a pair of oligonucleotide fragments which are respectively complementary with the 5 'end and the 3' end of the template are used as primers, under the action of DNA polymerase, the DNA molecule is extended along the template chain according to a semi-reserved replication mechanism until new DNA synthesis is completed, and the process is repeated, so that the target DNA fragment can be amplified.
At present, in the traditional PCR detection system, because the device has higher dependence on the result accuracy, and partial processes need manual operation during PCR detection, errors of results are probably caused by errors of manual operation, and time and labor are wasted.
Therefore, it is an urgent need to solve the problem of the art to provide a PCR detection system that can complete PCR detection by controlling other devices through a control device without manual operation.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a PCR detecting system, the device simple structure, safe, effective, reliable and easy and simple to handle, the error that can avoid manual operation causes the bad consequence that the error of result arouses.
Based on above purpose, the utility model provides a technical scheme as follows:
a PCR detection system comprises a frame, a temperature control device, an optical detection device, a chip fixing device and a control device, wherein the temperature control device, the optical detection device, the chip fixing device and the control device are arranged on the frame;
the control device is respectively connected with the temperature control device, the optical detection device and the chip fixing device;
the temperature control device is positioned below the chip fixing device;
the optical detection device is positioned above the chip fixing device and is used for detecting the condition of the amplified nucleic acid in the reaction cavity of the chip;
the control device is positioned above the optical detection device and used for driving the liquid in the chip to flow so as to realize the reaction and detection processes.
Preferably, the temperature control device comprises a heat sink and a peltier, one surface of the peltier is in close contact with the chip fixing device through heat-conducting glue, and the other surface of the peltier is fixed with the heat sink through heat-conducting glue.
Preferably, the frame includes a base plate, an outer support plate, a first fixing plate, a second fixing plate, and a third fixing plate;
the outer supporting plate is respectively and vertically connected with the base plate and the first fixing plate, and the base plate is parallel to the first fixing plate;
the temperature control device, the optical detection device, the chip fixing device and the control device are all arranged in a hollow inner cavity surrounded by the substrate, the outer support plate and the first fixing plate;
the third fixing plate is arranged between the temperature control device and the chip fixing device;
the second fixing plate is arranged between the control device and the chip fixing device;
the optical detection device and the chip fixing device are positioned between the third fixing plate and the second fixing plate.
Preferably, the temperature control device further comprises a cooling fan, an electromagnet fixing seat, a heat conducting fin, a heat insulating plate, a temperature sensor and a cooling fin supporting plate;
the radiating fin supporting plate is arranged between the base plate and the third fixing plate;
the heat radiation fan is arranged on the substrate and is positioned in a hollow inner cavity surrounded by the substrate, the third fixing plate and the heat radiation fin supporting plate;
the upper end of the heat radiation fan is provided with the electromagnet fixing seat;
the electromagnet fixing seat is connected with the lower surface of the third fixing plate through an electromagnet;
a heat conducting sheet is arranged on the upper surface of the third fixing plate, and the temperature sensor is arranged on the heat conducting sheet;
the heat insulation plate is covered on the heat conduction sheet, and the heat insulation plate is provided with a groove capable of accommodating the temperature sensor.
Preferably, the control device comprises a control motor, a control motor supporting plate and a control air tank;
the control motor is arranged on the upper surface of the second fixing plate;
the control motor supporting plate is vertically arranged between the second fixing plate and the first fixing plate;
and a sealing block is arranged on the heat insulation plate, and the control gas tank is fixed on the sealing block.
Preferably, the control device further comprises a chip pushing and gluing head, the chip pushing and gluing head is fixed on the control motor, and the chip pushing and gluing head is used for extruding the chip and driving liquid in the chip to flow.
Preferably, the control device further comprises a screw motor, a motor fixing plate and a sliding nut;
the screw rod motor is arranged on the first fixing plate;
the motor fixing plate is arranged below the first fixing plate;
the sliding nut is arranged on the motor fixing plate.
Preferably, the side wall of the control motor supporting plate is also provided with a linear slide rail and a high-pressure valve array;
the control gas tank is connected with the high-pressure valve array light path;
and the high-pressure valve array is respectively connected with the pneumatic pump and the pressure sensor.
Preferably, the optical detection device comprises an optical detection module, a photonic barrier sheet and an optoelectronic switch;
the optical detection module is arranged on the heat insulation plate;
the light blocking piece and the photoelectric switch are arranged on the side wall of the control motor supporting plate and are located on the same side as the linear sliding rail.
Preferably, the pneumatic pump is disposed on the substrate and located at one side of the control motor support plate.
The PCR detection system provided by the utility model is characterized in that the temperature control device, the optical detection device, the chip fixing device and the control device are arranged on the frame, and the control device is respectively connected with the temperature control device, the optical detection device and the chip fixing device; the temperature control device is arranged below the chip fixing device, the optical detection device is arranged above the chip fixing device, and the control device is arranged above the chip detection device. In the working process, after the control device is matched with the chip fixing device to fix the chip, liquid in the chip is driven to flow so as to realize the reaction and detection processes. Meanwhile, the temperature control device is controlled to be started through the control device, and the temperature of the environment around the chip reaction cavity is adjusted; and controlling the optical detection device to be started, and carrying out optical detection on the conditions of the amplified nucleic acid in the chip reaction cavities with different temperatures. The device simple structure can accomplish through controlling means a series of operations such as the fixed of chip, adjust the temperature, optical detection, the effectual detection efficiency that has improved, has avoided manual operation's error to cause the error of result, saves the cost.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present application, and for those skilled in the art, other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a PCR detection system provided by the present invention;
fig. 2 is a schematic structural diagram of a PCR detection system according to an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
The embodiment of the utility model provides an adopt progressive mode to write.
The embodiment of the utility model provides a PCR detecting system. The method mainly solves the technical problems that in the prior art, partial flow during PCR detection needs manual operation, and the manual operation may cause errors of results to precise PCR detection experiments.
A PCR detection system comprises a frame, and a temperature control device 1, an optical detection device 2, a chip fixing device 3 and a control device 4 which are arranged on the frame;
the control device 4 is respectively connected with the temperature control device 1, the optical detection device 2 and the chip fixing device 3;
the temperature control device 1 is positioned below the chip fixing device 3;
the optical detection device 2 is positioned above the chip fixing device 3 and is used for detecting the condition of the amplified nucleic acid in the reaction cavity of the chip;
the control device 4 is located above the optical detection device 2 and is used for driving the liquid in the chip to flow so as to realize the reaction and detection processes.
The PCR detection system provided by the utility model connects the control device 4 with the temperature control device 1, the optical detection device 2 and the chip fixing device 3 respectively by installing the temperature control device 1, the optical detection device 2, the chip fixing device 3 and the control device 4 on the frame; wherein the temperature control device 4 is arranged below the chip fixing device 3, the optical detection device 2 is arranged above the chip fixing device 3, and the control device 4 is arranged above the chip detection device 3. In the working process, after the control device 4 is matched with the chip fixing device 3 to fix the chip, the liquid in the chip is driven to flow so as to realize the reaction and detection processes. Meanwhile, the temperature control device 1 is controlled to be started through the control device 4, and the temperature of the surrounding environment of the chip reaction cavity is adjusted; and controlling the optical detection device 2 to be started, and carrying out optical detection on the conditions of the amplified nucleic acid of the chip reaction cavities with different temperatures. The device simple structure can accomplish through controlling means a series of operations such as the fixed of chip, adjust the temperature, optical detection, the effectual detection efficiency that has improved, has avoided manual operation's error to cause the error of result, saves the cost.
Preferably, the temperature control device 1 includes a heat sink and a peltier, one surface of the peltier is in close contact with the chip fixing device 3 through a heat conducting glue, and the other surface of the peltier is fixed to the heat sink through the heat conducting glue.
In the actual application process, the temperature control device 1 can be provided with a radiating fin and a Peltier, one surface of the Peltier is in close contact with the chip fixing device 3 through heat-conducting glue, and the other surface of the Peltier is fixed with the radiating fin through the heat-conducting glue. In the working process, the Peltier is used for providing rapid temperature circulation for a chip reaction cavity in the chip fixing device 3, the temperature is rapidly converted between 60 degrees and 90 degrees, and accurate and effective data are provided for nucleic acid amplification in the chip reaction cavity.
Preferably, the frame includes a base plate, an outer support plate, a first fixing plate, a second fixing plate, and a third fixing plate;
the outer supporting plate is respectively and vertically connected with the base plate and the first fixing plate, and the base plate is parallel to the first fixing plate;
the temperature control device 1, the optical detection device 2, the chip fixing device 3 and the control device 4 are all arranged in a hollow inner cavity surrounded by the substrate, the outer support plate and the first fixing plate;
the third fixing plate is arranged between the temperature control device 1 and the chip fixing device 3;
the second fixing plate is arranged between the control device 4 and the chip fixing device 3;
the optical detection device 2 and the chip fixing device 3 are located between the third fixing plate and the second fixing plate.
In the practical application process, the frame comprises a base plate, an outer supporting plate, a first fixing plate, a second fixing plate and a third fixing plate; a temperature control device 1, an optical detection device 2, a chip fixing device 3 and a control device 4 are arranged in a hollow inner cavity defined by the base plate, the first fixing plate and an outer supporting plate vertical to the base plate and the first fixing plate; a third fixing plate is arranged between the temperature control device 1 and the chip fixing device 3; a second fixing plate is arranged between the control device 4 and the chip fixing device 3; the optical detection device 2 and the chip fixing device 3 are located between the third fixing plate and the second fixing plate. In the working process, the uppermost control device 4 places the chip on the lower chip fixing device 3, and after the chip is fixed by the chip fixing device 3, the chip is optically detected by the optical detection device 2 on the same layer as the chip; the temperature control device 1 below the chip fixing device 3 is used for temperature control.
Preferably, the temperature control device 1 comprises a cooling fan 11, an electromagnet fixing seat 12, a heat conducting fin, a heat insulating plate 13, a temperature sensor 14 and a cooling fin supporting plate;
the radiating fin supporting plate is arranged between the substrate and the third fixing plate;
the heat radiation fan 11 is arranged on the substrate and is positioned in a hollow inner cavity surrounded by the substrate, the third fixing plate and the heat radiation fin supporting plate;
the upper end of the cooling fan 11 is provided with an electromagnet fixing seat 12;
the electromagnet fixing seat 12 is connected with the lower surface of the third fixing plate through an electromagnet;
the upper surface of the third fixing plate is provided with a heat conducting sheet, and the temperature sensor 14 is arranged on the heat conducting sheet;
the heat insulation plate 13 covers the heat conduction plate, and the heat insulation plate 13 is provided with a groove capable of accommodating the temperature sensor 14.
In the practical application process, the heat dissipation fan 11 in the temperature control device 1 is installed on the substrate and is located in a hollow inner cavity enclosed by the third fixing plate, the substrate and the heat dissipation fin supporting plate between the third fixing plate and the substrate; the heat dissipation fan 11 is used for dissipating heat of the hollow inner cavity; an electromagnet fixing seat 12 is arranged at the upper end of the radiating fan, and the electromagnet fixing seat 12 is connected with the lower surface of the third fixing plate through an electromagnet; the upper surface of the third fixing plate is provided with a heat conducting sheet, the temperature sensor 14 is arranged on the heat conducting sheet, and the heat insulation plate 13 is provided with a groove capable of accommodating the temperature sensor 14. In the working process, when the temperature needs to be reduced, the heat dissipation fan 11 is turned on to dissipate heat of the hollow inner cavity; the heat insulating board 13 prevents that the too high temperature of below from influencing the experiment precision, and temperature sensor 14 sets up the concave part in the middle of heat insulating board 13 for monitor below temperature, in time feed back adjusts.
Preferably, the control device 4 includes a control motor 41, a control motor support plate 42, and a control gas tank 43;
the control motor 41 is arranged on the upper surface of the second fixing plate;
the control motor supporting plate 42 is vertically arranged between the second fixing plate and the first fixing plate;
a sealing block is provided on the heat shield 13, and a control gas tank 43 is fixed to the sealing block.
In practical application, the control motor 41 of the control device 4 is arranged on the upper surface of the second fixing plate, and the control motor supporting plate 42 is vertically arranged between the second fixing plate and the first fixing plate; the control gas tank 43 in the control device is fixedly arranged on a sealing block mounted on the heat insulation plate 13. In the working process, the control motor 41 is arranged in a hollow inner cavity defined by the control motor supporting plate 42, the first fixing plate and the second fixing plate, and the control motor 41 in the working state detects a chip after the chip is fixed by the chip fixing device 3 under the control of the control gas tank 43 arranged on the sealing block.
Preferably, the control device 4 further comprises a chip pushing and gluing head, the chip pushing and gluing head is fixed on the control motor 41, and the chip pushing and gluing head is used for extruding the chip and driving liquid in the chip to flow.
In the actual application process, the control device 4 can also be provided with a chip pushing and gluing head, and the chip pushing and gluing head is fixed on the control motor 41. In the course of the work, after control motor 41 and chip fixing device 3 cooperate the fixed chip, control motor 41 drives the chip and bulldozes gluey first extrusion chip, orders about the interior liquid flow of chip to realize reaction and testing process.
Preferably, the control device 4 further comprises a lead screw motor 44, a motor fixing plate 45 and a slide nut 46;
the screw motor 44 is arranged on the first fixing plate;
the motor fixing plate 45 is arranged below the first fixing plate;
the slide nut 46 is provided on the motor fixing plate 45.
In practical application, a screw motor 44 in the control device is arranged on the first fixing plate, a motor fixing plate 45 is arranged below the first fixing plate and used for supporting a sliding nut 46 and the first fixing plate, and the sliding nut 46 is arranged on the motor fixing plate. In the working process, the screw motor drives the sliding nut 46 below the first fixing plate to rotate, so as to adjust the control motor 41.
Preferably, a linear slide rail 47 and a high-pressure valve array 48 are further arranged on the side wall of the control motor supporting plate 42;
the control gas tank 43 is connected with the high-pressure valve array 48 in a light path;
the high-pressure valve array 48 is respectively connected with the pneumatic pump and the pressure sensor.
In the practical application process, the side wall of the control motor supporting plate 42 is also provided with a linear slide rail 47 and a high-pressure valve array 48, and the high-pressure valve array 48 is connected with the light path of the control gas tank 43 and is respectively connected with a pneumatic pump and a pressure sensor; the linear slide 47 is used to fine tune the position of the high pressure valve array 48.
Preferably, the optical detection device 2 comprises an optical detection module 21, a photonic barrier 22 and an optoelectronic switch 23;
the optical detection module 21 is arranged on the heat insulation plate 13;
the light blocking plate 22 and the photoelectric switch 23 are both arranged on the side wall of the control motor supporting plate 42 and are positioned on the same side as the linear slide rail 47.
In the actual application process, the detection module 21 in the optical detection device 2 is arranged on the heat insulation board 13 and used for carrying out optical detection on the chip; the light blocking sheet 22 and the photoelectric switch 23 in the optical detection device 2 are both arranged on the control motor support plate and used for assisting optical detection.
Preferably, the pneumatic pump is disposed on the base plate at one side of the control motor support plate 42.
In practical application, a pneumatic pump may be separately disposed on the substrate, and the pneumatic pump is disposed on one side of the control motor support plate 42 for facilitating connection with the high-pressure valve array 48.
The PCR detection system provided by the present invention is described in detail above. The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (10)
1. A PCR detection system is characterized by comprising a frame, and a temperature control device (1), an optical detection device (2), a chip fixing device (3) and a control device (4) which are arranged on the frame;
the control device (4) is respectively connected with the temperature control device (1), the optical detection device (2) and the chip fixing device (3);
the temperature control device (1) is positioned below the chip fixing device (3);
the optical detection device (2) is positioned above the chip fixing device (3) and is used for detecting the condition of the amplified nucleic acid in the reaction cavity of the chip;
the control device (4) is positioned above the optical detection device (2) and used for driving the liquid in the chip to flow so as to realize the reaction and detection processes.
2. The PCR detection system according to claim 1, wherein the temperature control device (1) comprises a heat sink and a peltier, one side of the peltier is in close contact with the chip fixing device (3) through a heat conductive adhesive, and the other side of the peltier is fixed to the heat sink through a heat conductive adhesive.
3. The PCR detection system of claim 2, wherein the frame comprises a base plate, an outer support plate, a first fixing plate, a second fixing plate, and a third fixing plate;
the outer supporting plate is respectively and vertically connected with the base plate and the first fixing plate, and the base plate is parallel to the first fixing plate;
the temperature control device (1), the optical detection device (2), the chip fixing device (3) and the control device (4) are all arranged in a hollow inner cavity surrounded by the substrate, the outer support plate and the first fixing plate;
the third fixing plate is arranged between the temperature control device (1) and the chip fixing device (3);
the second fixing plate is arranged between the control device (4) and the chip fixing device (3);
the optical detection device (2) and the chip fixing device (3) are positioned between the third fixing plate and the second fixing plate.
4. The PCR detection system according to claim 3, wherein the temperature control device (1) further comprises a heat radiation fan (11), an electromagnet fixing seat (12), a heat conducting fin, a heat insulation plate (13), a temperature sensor (14) and a heat radiation fin supporting plate;
the radiating fin supporting plate is arranged between the base plate and the third fixing plate;
the heat radiation fan (11) is arranged on the substrate and is positioned in a hollow inner cavity surrounded by the substrate, the third fixing plate and the heat radiation fin supporting plate;
the upper end of the heat radiation fan (11) is provided with the electromagnet fixing seat (12);
the electromagnet fixing seat (12) is connected with the lower surface of the third fixing plate through an electromagnet;
a heat conducting sheet is arranged on the upper surface of the third fixing plate, and the temperature sensor (14) is arranged on the heat conducting sheet;
the heat insulation plate (13) is covered on the heat conduction plate, and the heat insulation plate (13) is provided with a groove capable of accommodating the temperature sensor (14).
5. The PCR detection system according to claim 4, wherein the control means (4) includes a control motor (41), a control motor support plate (42), and a control gas tank (43);
the control motor (41) is arranged on the upper surface of the second fixing plate;
the control motor supporting plate (42) is vertically arranged between the second fixing plate and the first fixing plate;
a sealing block is arranged on the heat insulation plate (13), and the control air tank (43) is fixed on the sealing block.
6. The PCR detection system according to claim 5, wherein the control device (4) further comprises a chip pushing glue head fixed on the control motor (41), and the chip pushing glue head is used for squeezing the chip to drive the liquid in the chip to flow.
7. The PCR detection system according to claim 5, wherein the control device (4) further comprises a lead screw motor (44), a motor fixing plate (45) and a slide nut (46);
the screw rod motor (44) is arranged on the first fixing plate;
the motor fixing plate (45) is arranged below the first fixing plate;
the sliding nut (46) is arranged on the motor fixing plate (45).
8. The PCR detection system of claim 7, wherein the control motor support plate (42) is further provided with a linear slide rail (47) and a high pressure valve array (48) on the side wall;
the control gas tank (43) is in optical connection with the high-pressure valve array (48);
and the high-pressure valve array (48) is respectively connected with the pneumatic pump and the pressure sensor.
9. PCR detection system according to claim 8, wherein the optical detection means (2) comprise an optical detection module (21), a Photonic plate (22) and an opto-electronic switch (23);
the optical detection module (21) is arranged on the heat insulation plate (13);
the light blocking sheet (22) and the photoelectric switch (23) are arranged on the side wall of the control motor supporting plate (42) and are located on the same side as the linear sliding rail (47).
10. The PCR detection system according to claim 9, wherein the pneumatic pump is disposed on the substrate at one side of the control motor support plate (42).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120473386.3U CN215250871U (en) | 2021-03-04 | 2021-03-04 | PCR detection system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120473386.3U CN215250871U (en) | 2021-03-04 | 2021-03-04 | PCR detection system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215250871U true CN215250871U (en) | 2021-12-21 |
Family
ID=79503016
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120473386.3U Active CN215250871U (en) | 2021-03-04 | 2021-03-04 | PCR detection system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215250871U (en) |
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2021
- 2021-03-04 CN CN202120473386.3U patent/CN215250871U/en active Active
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