CN115282089A - Ganoderma lucidum extract nanoemulsion and preparation method and application thereof - Google Patents
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Abstract
The invention discloses a ganoderma extract nanoemulsion and a preparation method and application thereof, wherein the ganoderma extract nanoemulsion consists of ganoderma triterpene, ganoderma polysaccharide, a surfactant, a cosurfactant, an oil phase and water, wherein the mass ratio of the surfactant to the cosurfactant is 1-2. The O/W type ganoderma lucidum extract nanoemulsion is prepared by screening the types and the use amounts of all components in a formula, and is sphere-like, stable in property, uniform in distribution and good in fluidity; the ganoderma extract nanoemulsion prepared by the invention obviously improves the solubility of ganoderma triterpene and ganoderma polysaccharide, is beneficial to improving the drug-loading rate of a system and the utilization rate of effective components thereof, is suitable for being applied to the field of cosmetics, and provides a certain experimental basis for the subsequent technological production and theoretical research of ganoderma nanoemulsion cosmetics.
Description
Technical Field
The invention relates to the technical field of cosmetics, and particularly relates to a ganoderma lucidum extract nanoemulsion and a preparation method and application thereof.
Background
Ganoderma lucidum is dry fruiting body of Ganoderma lucidum (Ganoderma lucid-um) or Ganoderma sinense (Ganoderma sinense Zhao, xu et Zhang) belonging to Polyporaceae, and has effects of nourishing, strengthening, prolonging life, and restoring vital energy. Modern medical research and experiments further prove that the lucid ganoderma has the functions of resisting oxidation and aging. Researches show that the ganoderan and the ganoderma triterpene are main effective components of ganoderma and are closely related to the efficacy and the function of the medicine.
The nano-emulsion is a stable system which is formed by oil, water, surfactant (SA), cosurfactant (CoSA) and the like, has the particle size of 1-100 nm, is clear and transparent, can generate the Tyndall effect, and can be bluish opalescent in some systems. The nanoemulsion as a novel drug delivery system can increase the solubility of the drug, prolong the action time of the drug and increase the bioavailability of the drug by virtue of the characteristics of the nanoemulsion.
At present, most of researches on ganoderma lucidum extracts are carried out on the preparation of ganoderma lucidum extract oral preparations, the effects of the ganoderma lucidum extracts are mainly immunoregulation, treatment, tumor prevention and the like, and no literature report for preparing external-use type nano-emulsions by the ganoderma lucidum extracts is found.
Disclosure of Invention
In view of this, the primary objective of the present invention is to provide a ganoderma lucidum extract nanoemulsion, which is sphere-like, uniformly distributed, and has better stability.
The invention is realized by the following technical scheme:
a ganoderma lucidum extract nanoemulsion consists of ganoderma lucidum triterpene, ganoderma lucidum polysaccharide, a surfactant, a cosurfactant, an oil phase and water, wherein the mass ratio of the surfactant to the cosurfactant is 1.
Preferably, the surfactant is one or more of polysorbate-80 (Tween 80), castor oil polyoxyethylene ether 40 (EL 40) or polyoxyethylene castor oil 35 (EL 35), and more preferably the polyoxyethylene castor oil 35 (EL 35).
Preferably, the cosurfactant is one or more of absolute ethyl alcohol, glycerol or propylene glycol, and more preferably glycerol.
Preferably, the oil phase is one or a mixture of Medium Chain Triglycerides (MCT) or isopropyl myristate (IPM), more preferably Medium Chain Triglycerides (MCT).
The ganoderma triterpene and the ganoderma polysaccharide can be obtained by a commercial method and can also be prepared by a conventional method in the field.
The average particle size of the ganoderma lucidum extract nanoemulsion prepared by the invention is 28.01nm, the PDI is 0.105, the average Zeta potential is-4.05 mV, the maximum drug loading of ganoderma lucidum polysaccharide in the ganoderma lucidum extract nanoemulsion is 36.35mg/mL, and the maximum drug loading of ganoderma lucidum triterpene is 5.99mg/mL.
The invention also provides a preparation method of the ganoderma lucidum extract nanoemulsion, which comprises the following steps:
a. uniformly mixing a surfactant and a cosurfactant to obtain a mixed surfactant, and uniformly mixing the mixed surfactant and an oil phase to obtain a mixed system;
b. under the stirring state, dropwise adding water into the mixed system, and stirring until the mixed system is clear to obtain blank nanoemulsion;
c. adding Ganoderma triterpene into blank nanoemulsion, standing, centrifuging to obtain supernatant, dissolving Ganoderma polysaccharide in the supernatant, and stirring to dissolve completely to obtain Ganoderma extract nanoemulsion.
Preferably, steps (a) - (c) are carried out in a water bath at 50-60 ℃ and with a magnetic stirring speed of 1000-1500 r/min.
The third aspect of the invention provides the application of the ganoderma extract nanoemulsion in the external skin care preparation, the invention combines the ganoderma extract and the nanoemulsion to prepare the external skin care preparation with anti-aging and anti-oxidation effects, which not only can increase the solubility of the ganoderma effective components and improve the utilization value of ganoderma, but also can promote the transdermal absorption of the effective components of the ganoderma to achieve the effects of beautifying and anti-aging due to the unique advantages of the nanoemulsion preparation.
Preferably, the external skin care preparation is essence, lotion, cream, mask, lotion or spray.
Compared with the prior art, the invention has the following beneficial effects:
(1) The O/W type ganoderma lucidum extract nanoemulsion is prepared by screening the types and the use amounts of all components in a formula, and is sphere-like, stable in property, uniform in distribution and good in fluidity;
(2) The ganoderma lucidum extract nanoemulsion prepared by the invention obviously improves the solubility of ganoderma lucidum triterpene and ganoderma lucidum polysaccharide, and is beneficial to improving the drug-loading rate of a system and the utilization rate of effective components thereof;
(3) The invention combines the ganoderma lucidum extract and the nanoemulsion, is suitable for being applied to the field of cosmetics, can prepare an external skin care preparation with anti-aging and anti-oxidation effects, can increase the solubility of ganoderma lucidum active ingredients and improve the utilization value of ganoderma lucidum, can promote the transdermal absorption of the active ingredients of the nano-emulsion preparation to achieve the effects of beautifying and resisting aging due to the unique advantages of the nano-emulsion preparation, and provides a certain experimental basis for the subsequent technological production and theoretical research of ganoderma lucidum extract nano-emulsion cosmetics.
Drawings
FIG. 1 is a pseudo-ternary phase diagram for different surfactants SA;
FIG. 2 is a pseudo-ternary phase diagram for different km values for surfactant SA and co-surfactant;
FIG. 3 is a pseudo-ternary phase diagram of different oil phases;
FIG. 4 is a standard curve of ganoderan;
FIG. 5 is a standard curve of Ganoderma triterpene;
FIG. 6 is an appearance examination of a Ganoderma lucidum extract nanoemulsion;
FIG. 7 shows the Tyndall effect of the Ganoderma lucidum extract nanoemulsion;
FIG. 8 shows the type identification of the Ganoderma lucidum extract nanoemulsion;
FIG. 9 shows the morphology distribution of Ganoderma extract nanoemulsion under transmission electron microscope.
Detailed Description
The present invention is further illustrated by the following specific examples, which are not intended to limit the scope of the invention.
[ PREPARATION OF LINGZHITANOSE ]
Extracting for 2h at 70 ℃ by adopting a water bath extraction method, wherein the material-liquid ratio is 1 (W/V), so as to obtain the total polysaccharide solution of the ganoderma lucidum fruiting body. Concentrating by rotary evaporation, adding ethanol into the extractive solution until the ethanol content is 80%, stirring, standing at 4 deg.C for 24 hr, centrifuging, removing supernatant, and volatilizing ethanol in precipitate to obtain crude polysaccharide solid of fruiting body.
[ PREPARATION OF TRITERPENE OF LINGZHI ]
Accurately weighing 100g of ganoderma lucidum fruiting body coarse powder by adopting an ethanol extraction method, putting the ganoderma lucidum fruiting body coarse powder into a round-bottom flask, and mixing the ganoderma lucidum fruiting body coarse powder and the round-bottom flask according to a material-liquid ratio of 1:20 (W/V) adding anhydrous ethanol, and extracting under reflux at 70 deg.C for 2 times, each for 1.5 hr. The extract was collected by suction filtration and shaken up. And carrying out rotary evaporation and concentration on the obtained extract liquid at 55 ℃ to obtain an extract, and storing for later use.
Example 1:
(1) Uniformly mixing EL 35.7 g and glycerol 1.3g to obtain a mixed surfactant (Smix), adding MCT 1.0g into Smix, and uniformly mixing in a magnetic stirring water bath kettle at 55 deg.C and at a rotation speed of 1000-1500r/min to obtain a mixed system;
(2) Dropwise adding 4.0g of water into the mixed system under the stirring state, and stirring until the mixed system is clear to obtain a blank nano-emulsion;
(3) Adding excessive Ganoderma triterpene into blank nanoemulsion a little for multiple times, standing for 24 hr, separating precipitate from nanoemulsion after loading drug by low speed centrifugation (2000 rpm), and keeping supernatant; dissolving Ganoderma polysaccharide powder in the supernatant for several times, and stirring to dissolve completely to obtain brown yellow clear transparent Ganoderma extract nanometer emulsion.
Example 2:
(1) Uniformly mixing 3.75g of EL and 3.75g of glycerol to obtain a mixed surfactant (Smix), adding 1.25g of MCT into the Smix, and uniformly mixing in a magnetic stirring water bath kettle at 50 ℃ and at the rotating speed of 1000-1500r/min to obtain a mixed system;
(2) Dropwise adding 1.25g of water into the mixed system under the stirring state, and stirring until the mixed system is clear to obtain a blank nano-emulsion;
(3) Adding excessive Ganoderma triterpene into blank nanoemulsion a little for many times, standing for 24h, separating precipitate from the nanoemulsion after loading medicine by low speed centrifugation (2000 rpm), and keeping supernatant; dissolving Ganoderma polysaccharide powder in the supernatant for several times, and stirring to dissolve completely to obtain brown yellow clear transparent Ganoderma extract nanometer emulsion.
Example 3:
(1) Uniformly mixing 4.2g of EL and 2.1g of glycerol to obtain a mixed surfactant (Smix), adding 2.5g of IPM into the Smix, and uniformly mixing in a magnetic stirring water bath kettle at 60 ℃ and at the rotating speed of 1000-1500r/min to obtain a mixed system;
(2) Dropwise adding 1.25g of water into the mixed system under the stirring state, and stirring until the mixed system is clear to obtain blank nano-emulsion;
(3) Adding excessive Ganoderma triterpene into blank nanoemulsion a little for many times, standing for 24h, separating precipitate from the nanoemulsion after loading medicine by low speed centrifugation (2000 rpm), and keeping supernatant; dissolving Ganoderma polysaccharide powder in the supernatant for several times, and stirring to dissolve completely to obtain brown yellow clear transparent Ganoderma extract nanometer emulsion.
Example 4: prescription screening of nanoemulsion
4.1 selection of Surfactant (SA)
4.1.1 surfactant stability test
When the HLB value is 3-6, the emulsifier is W/O type, when the HLB value is 8-18, the emulsifier is O/W type, and according to the HLB value calculation formula of CoSA: HLB = HLB A *A％+HLB B * B%, the invention preselects Tween80, RH40, EL40 and EL35 as alternative SA, the HLB value of the SA ranges from 13 to 16, and glycerol is selected as CoSA. Mixing Tween80, RH40, EL40 and EL35 with glycerol according to a ratio of 1. The reagents are mixed uniformly according to the above operation, and the mixture is placed at 4 ℃, room temperature (25 ℃) and 55 ℃ respectively, and the color, clarity, viscosity, whether layering exists or not and the like of the mixture are observed after 10 min. After being mixed, the SA which is clear, transparent, non-layered and low in viscosity is selected as the alternative SA of the nano-emulsion. The results are given in the following table (table 3).
TABLE 3 stability of the formulations of the different mixed surfactants
From the results, it is known that the mixed solvent of RH40 and glycerol is milky white and has high viscosity under different conditions, and the mixed solvent of Tween80, EL40, EL35 and glycerol is clear and transparent, has low viscosity and is not layered under various conditions, so Tween80, EL40, and EL35 are selected as alternative SA for the next experimental operation.
4.1.2 selection of optimal surfactant
Uniformly mixing Tween80, EL40 and EL35 with glycerol according to a mass ratio of km =2 mix And mixing the emulsion and the oil phase uniformly according to the mass ratio of 1.
As can be seen from FIG. 1, tween80 and EL40 are not easy to form milk in the high oil phase region, and the areas of the milk forming regions are sequentially from large to small: EL35> EL40> Tween80, EL35 emulsion forming zone area is largest, so EL35 is preferably SA of nanoemulsion.
4.2 cosurfactant (CoSA) determination and Km value determination
CoSA which is commonly used in nanoemulsion comprises absolute ethyl alcohol, glycerol, propylene glycol and the like, and the fat-soluble ganoderma triterpene compound used in the invention is prepared by extracting absolute ethyl alcohol, but absolute ethyl alcohol has certain irritation to skin and is not suitable for being used as an external skin care product, and glycerol is harmless to skin, can be dissolved with a plurality of substances and has the effects of water absorption, moisture retention and oxidation resistance, so the ganoderma triterpene compound can be used as a solvent, a skin moistening agent, a moisturizing agent and the like in various skin care products.
In summary, glycerol was selected as CoSA in this experiment. And (2) mixing SA and CoSA uniformly according to the following ratio of Km =1, 1 mix And mixing the emulsion and the oil phase uniformly according to the mass ratio of 1.
As can be seen from fig. 2, the pseudo-ternary diagram has the largest emulsion forming area when km = 2.
4.3 selection of the oil phase
4.3.1 solubility test of oil phase
MCT (medium chain triglyceride), IPM (isopropyl myristate) and olive oil are common oil phases in cosmetics, and MCT is good in lubricating property, low in viscosity and capable of being mutually dissolved with other solvents; the IPM is good in ductility and fresh and has the functions of moisturizing; the olive oil is vegetable oil, is common in external cosmetics, and has natural skin caring effect.
The invention preselects the three oil phases as the oil phase to be selected. Because the ganoderma triterpene is a fat-soluble compound, the ganoderma triterpene extract is dissolved in each oil phase, and the solubility and the stability of the ganoderma triterpene in each oil phase are examined. Adding excessive Ganoderma triterpene into the three oil phases, and vortexing the mixture with a vortexer to accelerate the mixing of the medicine and various solvents. Vortex 30s every 5min and observe dissolution within 30min until the drug is no longer dissolved and some solid precipitates after standing. And then placing each centrifuge tube after being fully mixed in a water bath constant temperature oscillator at 25 +/-2 ℃ for continuously shaking for 24 hours, and observing the dissolution condition. If solid precipitate still exists, the solution is in a saturated state, and the ganoderma triterpene saturated oil solution is obtained. The saturated solution was centrifuged at 12000r/min for 10min to remove excess drug. Taking the mother liquor, and measuring the content of the ganoderma triterpene in the mother liquor by using an ultraviolet spectrophotometry. Thereby screening the SA candidate agent with better solubility. Experiments show that the ganoderma triterpene has the solubility of 1.7mg/ml in MCT and 2.8mg/ml in IPM, but has poor solubility in olive oil, and obvious layering phenomenon can occur after centrifugation, so the MCT and the IPM are selected as oil phases to be selected.
4.3.2 selection of optimal oil phase
Uniformly mixing EL35 with glycerol according to the mass ratio of km =2 mix Mixing with the candidate oil phase MCT, IPM in the mass ratio of 1.
As can be seen from fig. 3, the emulsion-forming area MCT > IPM, and therefore MCT was chosen as the best oil phase for this nanoemulsion formulation.
In summary, the optimal formula for this nanoemulsion was SA for EL35, coSA for glycerol, 2 km for km. And the formula proportion of the most stable nanoemulsion screened from the emulsion forming area of the pseudo-ternary phase diagram is as follows: EL 35:30%, glycerin: 14.4%, MCT:11.1%, water: 44.5 percent.
Example 5 quality evaluation of Ganoderma lucidum extract nanoemulsion (example 1)
5.1 nanoemulsion drug loading assay
Dissolving the prepared ganoderma extract nanoemulsion in methanol for demulsification, measuring absorbance values of ganoderma polysaccharide and triterpene solutions in the nanoemulsion at 626nm and 546nm respectively by using an ultraviolet spectrophotometry, and contrasting with a standard curve to obtain the drug loading content of the ganoderma polysaccharide and the triterpene in the nanoemulsion.
5.1.1 spectrophotometry for determining ganoderan content
Precisely weighing 2.5mg of D- (+) -glucose, adding pure water to a constant volume of 25mL, and preparing a glucose standard solution of 0.1 mg/mL. Accurately weighing 25mg of anthrone, adding concentrated sulfuric acid to a constant volume of 25mL, and preparing sulfuric acid-anthrone solution. Precisely measuring 0.2mL, 0.4 mL, 0.6 mL, 0.8mL and 1.0mL of glucose standard solution, adding pure water to 1.0mL, respectively adding 3.0mL of sulfuric acid-anthrone solution, immediately mixing well, and reacting at room temperature for 5min. The absorbance values were determined at a wavelength of 626nm under a blank control. And (4) taking the standard glucose content as an X axis and the absorbance value as a Y axis to obtain a standard curve and a regression equation. The results are shown in FIG. 4.
Precisely measuring 1.0mL of ganoderma extract nanoemulsion, diluting by 100 times with methanol, ultrasonically demulsifying for 10min, uniformly mixing, precisely measuring 0.17mL of methanol-diluted demulsified polysaccharide solution from the solution, adding methanol to 1.0mL into another test tube, adding 3.0mL of sulfuric acid-anthrone solution, immediately uniformly mixing, and reacting for 5min at room temperature. The absorbance values were determined at a wavelength of 626nm under a blank control. The measurement was performed 3 times in parallel, and the average value was obtained. And substituting the regression equation of the obtained ganoderma lucidum polysaccharide concentration to obtain the ganoderma lucidum polysaccharide content in the nano milk of 36.35mg/ml.
5.1.2 spectrophotometry for determining content of ganoderma triterpene
Adding methanol into oleanolic acid to prepare a standard solution with the content of 0.2 mg/ml. Taking 0.1,0.2,0.3,0.4 and 0.5mL of the above solution, respectively placing in 10.0mL test tubes with stoppers, volatilizing (55 ℃), cooling, adding 0.2mL of newly prepared 5% vanillin glacial acetic acid solution and 0.8mL of perchloric acid, uniformly mixing, carrying out water bath (70 ℃) for 15min, immediately carrying out ice bath for 5min, taking out, adding 4mL of ethyl acetate as soon as possible, and uniformly mixing. Under blank control, at a wavelength of 546nm, measuring absorbance value, taking mass concentration as X axis and absorbance as Y axis, and obtaining standard curve and regression equation, with the result shown in FIG. 5.
Accurately measuring 1.0mL of ganoderma extract nanoemulsion, diluting 100 times with methanol, ultrasonically demulsifying for 10min, uniformly mixing, taking 0.2mL of a sample to be measured, putting the sample into a 10mL test tube with a plug, performing the same operation according to the steps from 'volatilizing to dry', measuring absorbance, measuring each sample for 3 times in parallel, and taking an average value and recording. Substituting into the regression equation of the obtained concentration of ganoderma triterpene to obtain the ganoderma triterpene content in the nano milk of 5.99mg/ml.
The results show that the solubility of the ganoderma triterpene in the oil phase is 1.70mg/ml, the solubility of the ganoderma polysaccharide in the water is 21.72mg/ml, and the solubilities of the ganoderma triterpene and the ganoderma polysaccharide in the nano milk are 5.99mg/ml and 36.35mg/ml respectively. Experimental results show that the nanoemulsion obviously improves the solubility of ganoderma triterpene and ganoderma polysaccharide, and is beneficial to improving the drug-loading rate of a system and the utilization rate of effective components of the system.
Preparing the ganoderma extract nanoemulsion with the polysaccharide content of 6.00mg/ml and the triterpene content of 5.99mg/ml for subsequent experiments.
5.2 appearance examination of nanoemulsion
Placing the blank nanoemulsion and the Ganoderma extract nanoemulsion in containers respectively, as shown in FIG. 6, wherein the blank nanoemulsion is clear transparent emulsion and has light blue opalescence (A); the nano-emulsion added with ganoderma triterpene is light yellow clear emulsion (B); the solution after adding the ganoderma triterpene and the ganoderma polysaccharide is dark brown clear emulsion (C).
The Tyndall effect is the phenomenon that a visible 'light band' is generated by colloidal emulsion with the particle size of 1-100 nm under the irradiation of convergent light. Therefore, when laser is irradiated from the side, the nano emulsion generates a relatively obvious Tyndall effect, and the nano emulsion prepared by the experiment can be proved to meet the requirement of nano-scale particle size. As shown in fig. 7.
5.3 type identification of nanoemulsion
The method for identifying the type of the nano-emulsion comprises a dilution method, a dyeing method, a conductivity method and the like. The dyeing method is selected for the experiment because of simple operation and obvious phenomenon. The methylene blue solution is selected as the identification reagent, because the methylene blue solution is water-soluble, can rapidly diffuse in O/W type emulsion, has obvious phenomenon which is easy to observe, and cannot diffuse in W/O type emulsion. The nanoemulsion prepared in the experiment is taken and put in an EP tube, a proper amount of methylene blue solution is dripped into the EP tube, the phenomenon is observed and recorded (figure 8), and the experimental result shows that blue color is rapidly diffused after the methylene blue solution is dripped into the nanoemulsion, so the ganoderma lucidum extract nanoemulsion prepared in the experiment is O/W type nanoemulsion.
5.4 particle size distribution and Zeta potential
The results of taking 1ml of the ganoderma extract nanoemulsion, diluting the nanoemulsion with pure water by 20 times, respectively placing appropriate amounts of the ganoderma extract nanoemulsion in respective sample cells, and measuring the average particle size and the Zeta potential by using a laser particle sizer are shown in the table (table 4).
The average grain diameter of the nano-emulsion is 28.01nm, the PDI is 0.105, the prepared nano-emulsion has uniform grain diameter, uniform distribution and stable system, meets the standard grain diameter range of the nano-emulsion, and the Zeta potential is-4.05 mV. Zeta potential refers to the difference in potential between the continuous phase and the fluid stabilization layer attached to the dispersed particles, the larger the absolute value, the more stable the system. The Zeta potential result of the nano-emulsion prepared by the experiment shows that the electrostatic repulsion force of nano-emulsion particles is larger, namely the whole nano-emulsion system is more stable.
TABLE 4 mean particle size, PDI, zeta potential of Ganoderma lucidum extract nanoemulsion
5.5 morphological examination
Diluting the Ganoderma extract nanoemulsion to appropriate concentration, dripping one drop on copper mesh, soaking for about 1min, and sucking off the excessive liquid with filter paper to naturally dry; and then dripping 1 drop of 3% phosphotungstic acid solution, dyeing for about 5min, naturally airing the residual solution after absorbing the residual solution by filter paper, and observing the prepared copper mesh by using a transmission electron microscope. The results (as shown in fig. 9) show that the ganoderma lucidum extract nanoemulsion prepared by the experiment is in a sphere-like shape and is uniformly distributed.
5.6 centrifugal stability
In the experiment, the high-speed centrifugation method is adopted to investigate the centrifugal stability of the ganoderma extract nanoemulsion, and whether the ganoderma extract nanoemulsion can have the phenomena of standing layering, uneven distribution, aggregation and sedimentation and the like is observed. And centrifuging the sample at 9000r/min for 15min, wherein the result shows that the phenomena of layering, precipitation and the like do not occur in the nanoemulsion after high-speed centrifugation. Therefore, the nano-emulsion prepared by the experiment has better stability.
5.7 dilution stability
The nanoemulsion prepared by the experiment is subjected to dilution stability investigation, and after the ganoderma extract nanoemulsion is diluted by pure water by 50 times, 100 times, 500 times and 1000 times respectively, no phenomena such as layering, demulsification, flocculation and the like are observed, and the nanoemulsion prepared by the experiment is diluted by 500 times to reach the condition of infinite dilution, so that the stability of the nanoemulsion prepared by the experiment cannot be influenced and can be infinitely diluted. Meanwhile, the structure type of the ganoderma lucidum extract nanoemulsion is further proved to be an O/W type nanoemulsion.
Claims (9)
1. The ganoderma lucidum extract nanoemulsion is characterized by consisting of ganoderma lucidum triterpene, ganoderma lucidum polysaccharide, a surfactant, a cosurfactant, an oil phase and water, wherein the mass ratio of the surfactant to the cosurfactant is 1-2.
2. The ganoderma lucidum extract nanoemulsion according to claim 1, wherein the surfactant is one or more of polysorbate-80, castor oil polyoxyethylene ether 40 or polyoxyethylene castor oil 35, preferably polyoxyethylene castor oil 35.
3. The ganoderma lucidum extract nanoemulsion of claim 1, wherein the cosurfactant is one or more of absolute ethyl alcohol, glycerol or propylene glycol, and is preferably glycerol.
4. The ganoderma lucidum extract nanoemulsion of claim 1, wherein the oil phase is one or a mixture of two of medium-chain triglyceride and isopropyl myristate, preferably medium-chain triglyceride.
5. The ganoderma lucidum extract nanoemulsion of claim 1, wherein the ganoderma lucidum extract nanoemulsion has an average particle size of 28.01nm, a pdi of 0.105, an average Zeta potential of-4.05 mV, the maximum drug loading of ganoderma lucidum polysaccharide in the ganoderma lucidum extract nanoemulsion is 36.35mg/mL, and the maximum drug loading of ganoderma lucidum triterpene is 5.99mg/mL.
6. The method for preparing the ganoderma lucidum extract nanoemulsion of any one of claims 1 to 5, comprising the following steps:
a. uniformly mixing a surfactant and a cosurfactant to obtain a mixed surfactant, and uniformly mixing the mixed surfactant and an oil phase to obtain a mixed system;
b. under the stirring state, dropwise adding water into the mixed system, and stirring until the mixed system is clear to obtain blank nanoemulsion;
c. adding Ganoderma triterpene into blank nanoemulsion, standing, centrifuging to obtain supernatant, dissolving Ganoderma polysaccharide in the supernatant, and stirring to dissolve completely to obtain Ganoderma extract nanoemulsion.
7. The method for preparing ganoderma lucidum extract nanoemulsion of claim 6, wherein the steps (a) - (c) are performed in a water bath at 50-60 ℃ and under the condition of magnetic stirring rotation speed of 1000-1500 r/min.
8. Use of the ganoderma lucidum extract nanoemulsion of any one of claims 1-5 in an external skin care preparation.
9. The use according to claim 8, wherein the external skin care preparation is a serum, a lotion, a cream, a mask, a lotion or a spray.
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| CN102058530A (en) * | 2010-12-14 | 2011-05-18 | 于荣敏 | Ganoderma lucidum polysaccharide oral nanoemulsion and preparation method thereof |
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| CN109568354A (en) * | 2019-02-15 | 2019-04-05 | 中国科学院上海药物研究所 | A kind of composition and application thereof comprising ganoderma lucidum spore oil and ganoderma lucidum polysaccharide |
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| CN109876023B (en) * | 2019-03-08 | 2021-10-19 | 暨南大学 | Ganoderma lucidum spore oil nanoemulsion and preparation method and application thereof |
| CN110613643A (en) * | 2019-09-21 | 2019-12-27 | 云南辰锦威生物科技有限公司 | Repair wrinkle-removing cream and preparation method thereof |
| CN115282089A (en) * | 2022-08-02 | 2022-11-04 | 安徽中医药大学 | Ganoderma lucidum extract nanoemulsion and preparation method and application thereof |
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