CN115281194B - Bactericide containing fluxapyroxad and trifloxystrobin - Google Patents
Bactericide containing fluxapyroxad and trifloxystrobin Download PDFInfo
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- CN115281194B CN115281194B CN202211029822.3A CN202211029822A CN115281194B CN 115281194 B CN115281194 B CN 115281194B CN 202211029822 A CN202211029822 A CN 202211029822A CN 115281194 B CN115281194 B CN 115281194B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/50—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids the nitrogen atom being doubly bound to the carbon skeleton
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/56—1,2-Diazoles; Hydrogenated 1,2-diazoles
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The invention provides a bactericide containing fluxapyroxad and trifloxystrobin, and belongs to the technical field of bactericides. The invention provides a polysaccharide of Padina japonica, which can effectively inhibit the growth of hyphae of Botrytis cinerea and effectively inhibit the spore germination of Botrytis cinerea, so that the polysaccharide of Padina japonica can be used for preparing bactericides for preventing and treating Botrytis cinerea. Meanwhile, the invention provides a bactericide containing the sector algae polysaccharide, the fluxapyroxad and the trifloxystrobin, and better sterilizing effect is realized through compounding.
Description
Technical Field
The invention belongs to the technical field of bactericides, and particularly relates to a bactericide containing fluxapyroxad and trifloxystrobin.
Background
Gray mold is a worldwide crop disease, also known as gray rot or white spot, caused by botrytis cinerea. Gray mold can infect more than 400 plants including cucurbitaceae, solanaceae, rosaceae and the like, and is a low-temperature high-humidity disease, the temperature is low, the planting density is too high, the irrigation amount is too much, and the occurrence of gray mold can be accelerated due to poor field light transmission conditions. At present, the gray mold is mainly prevented and treated by chemical pesticides, but the long-term use of the chemical pesticides can lead to the generation of drug-resistant strains, so that the drug effect is reduced, and the environment is polluted. Therefore, the selection of new green pesticides is a current urgent need.
Haemophilus is a plant of the genus Paemonius, mainly distributed in Guangdong and Fujian coasts, algal polysaccharide is an important polysaccharide type, and no related research on the function of Paemonius polysaccharide exists at present.
Disclosure of Invention
The invention aims to provide a novel bactericide for gray mold control. The invention provides the following technical scheme for realizing the purposes:
the invention provides an application of sector algae polysaccharide in preparing gray mold treatment medicines, and the sector algae polysaccharide preparation method comprises the following steps:
(1) Placing the washed and dried large-area sector algae into a pulverizer, and pulverizing to obtain large-area sector algae powder;
(2) Placing the Padina pavonica powder into 30 times of purified water, and heating in water bath at 70deg.C for 5 hr to obtain Padina pavonica extract;
(3) Cooling to room temperature, and filtering the sector algae extracting solution to obtain sector algae filtrate;
(4) Concentrating the sector algae filtrate to 1/5 of the original volume, adding 1/3 volume of sevage reagent for deproteinization, and repeating for 5 times;
(5) According to 1:4, adding 95% ethanol in proportion, shaking uniformly, precipitating with ethanol for 24 hours, and centrifuging to obtain crude polysaccharide of the sector algae;
(6) And (3) dissolving the obtained crude polysaccharide of the sector algae, filtering by using an ultrafiltration membrane with a molecular weight cut-off of 100ku, collecting the cut-off liquid, and freeze-drying to obtain the polysaccharide of the sector algae.
Secondly, the invention provides application of the sector algae polysaccharide in preparing an ash mold inhibitor, and the sector algae polysaccharide preparation method comprises the following steps:
(1) Placing the washed and dried large-area sector algae into a pulverizer, and pulverizing to obtain large-area sector algae powder;
(2) Placing the Padina pavonica powder into 30 times of purified water, and heating in water bath at 70deg.C for 5 hr to obtain Padina pavonica extract;
(3) Cooling to room temperature, and filtering the sector algae extracting solution to obtain sector algae filtrate;
(4) Concentrating the sector algae filtrate to 1/5 of the original volume, adding 1/3 volume of sevage reagent for deproteinization, and repeating for 5 times;
(5) According to 1:4, adding 95% ethanol in proportion, shaking uniformly, precipitating with ethanol for 24 hours, and centrifuging to obtain crude polysaccharide of the sector algae;
(6) And (3) dissolving the obtained crude polysaccharide of the sector algae, filtering by using an ultrafiltration membrane with a molecular weight cut-off of 100ku, collecting the cut-off liquid, and freeze-drying to obtain the polysaccharide of the sector algae.
Third, the invention provides an application of the sector algae polysaccharide in preparing an inhibitor of the spore germination of the botrytis cinerea, and the preparation method of the sector algae polysaccharide comprises the following steps:
(1) Placing the washed and dried large-area sector algae into a pulverizer, and pulverizing to obtain large-area sector algae powder;
(2) Placing the Padina pavonica powder into 30 times of purified water, and heating in water bath at 70deg.C for 5 hr to obtain Padina pavonica extract;
(3) Cooling to room temperature, and filtering the sector algae extracting solution to obtain sector algae filtrate;
(4) Concentrating the sector algae filtrate to 1/5 of the original volume, adding 1/3 volume of sevage reagent for deproteinization, and repeating for 5 times;
(5) According to 1:4, adding 95% ethanol in proportion, shaking uniformly, precipitating with ethanol for 24 hours, and centrifuging to obtain crude polysaccharide of the sector algae;
(6) And (3) dissolving the obtained crude polysaccharide of the sector algae, filtering by using an ultrafiltration membrane with a molecular weight cut-off of 100ku, collecting the cut-off liquid, and freeze-drying to obtain the polysaccharide of the sector algae.
Fourth, the invention provides a preparation method of the sector algae polysaccharide for inhibiting gray mold, which comprises the following steps:
(1) Placing the washed and dried large-area sector algae into a pulverizer, and pulverizing to obtain large-area sector algae powder;
(2) Placing the Padina pavonica powder into 30 times of purified water, and heating in water bath at 70deg.C for 5 hr to obtain Padina pavonica extract;
(3) Cooling to room temperature, and filtering the sector algae extracting solution to obtain sector algae filtrate;
(4) Concentrating the sector algae filtrate to 1/5 of the original volume, adding 1/3 volume of sevage reagent for deproteinization, and repeating for 5 times;
(5) According to 1:4, adding 95% ethanol in proportion, shaking uniformly, precipitating with ethanol for 24 hours, and centrifuging to obtain crude polysaccharide of the sector algae;
(6) And (3) dissolving the obtained crude polysaccharide of the sector algae, filtering by using an ultrafiltration membrane with a molecular weight cut-off of 100ku, collecting the cut-off liquid, and freeze-drying to obtain the polysaccharide of the sector algae.
Fifth, the present invention provides a pesticidal composition for controlling gray mold, which comprises an active ingredient and an auxiliary ingredient.
Preferably, the ratio of the active ingredient to the auxiliary ingredient is 1:4.
preferably, the active ingredients include sector algae polysaccharide, fluxapyroxad and trifloxystrobin.
Preferably, the mass ratio of the fanner polysaccharide, the fluxapyroxad and the trifloxystrobin is 5:3:2.
Preferably, the auxiliary components comprise one or more of a solvent, a dispersant, a wetting agent, a thickener and an emulsifier.
The beneficial effects of the invention are as follows:
the invention discovers that the polysaccharide of the sector fan algae can effectively inhibit the diameter of the mycelium of the gray mold and can effectively inhibit the germination quantity of the spores of the gray mold. Therefore, it can be used as a novel bactericide for treating gray mold.
Drawings
FIG. 1 is the effect of Padina macrophylla polysaccharide on the diameter of Botrytis cinerea hyphae;
FIG. 2 is a graph showing the effect of Padina macrophylla polysaccharide on the germination count of Botrytis cinerea spores.
Detailed Description
In order to clearly illustrate the technical characteristics of the scheme, the scheme is explained below through a specific embodiment.
Example 1
(1) Placing the cleaned sector algae in a 60 ℃ oven for drying until the sector algae with constant weight is placed in a pulverizer, and pulverizing to obtain sector algae powder;
(2) Placing the Padina pavonica powder into 30 times of purified water, and heating in water bath at 70deg.C for 5 hr to obtain Padina pavonica extract;
(3) Cooling to room temperature, and filtering the sector algae extracting solution to obtain sector algae filtrate;
(4) Concentrating the sector algae filtrate to 1/5 of the original volume, adding 1/3 volume of sevage reagent for deproteinization, and repeating for 5 times;
(5) According to 1:4 adding 95% ethanol in proportion, shaking, precipitating with ethanol for 24h, centrifuging at 3500rpm in a centrifuge for 20min to obtain precipitate which is coarse polysaccharide of Padina macrophylla;
(6) And (3) dissolving the obtained crude polysaccharide of the sector algae, filtering by using an ultrafiltration membrane with a molecular weight cut-off of 100ku, collecting the cut-off liquid, and freeze-drying to obtain the polysaccharide of the sector algae.
Example 2
Inhibition of Botrytis cinerea polysaccharide on growth of Botrytis cinerea mycelium
(1) Adding Padina macrophylla polysaccharide into PDA culture medium to obtain culture medium with concentration of 0,30,60,120mg/ml, pouring the culture medium into culture dish, and taking 0mg/ml as control group;
(2) After the culture medium is solidified, a puncher is used for taking the strains with vigorous growth at the edge of a colony with the diameter of 6mm, and the strains are placed on a PDA flat plate for culture;
(3) After culturing in an incubator at 25℃for 72 hours, the diameter of each colony was measured using the crisscross method, the average value was used to represent the diameter of the colony, and the rate of inhibition of hypha growth was determined using the following formula:
inhibition of hypha growth rate = (control group colony diameter-treatment group colony diameter)/(control group colony diameter-0.6) ×100%;
TABLE 1 inhibition of Botrytis cinerea polysaccharide
| Concentration of | Hypha diameter | Inhibition rate |
| 0 | 6.12±0.21 | - |
| 30 | 4.59±0.18 | 27.72% |
| 60 | 3.45±0.16 | 48.37% |
| 120 | 1.46±0.19 | 84.42% |
The experimental results of fig. 1 and table 1 show that the polysaccharide of the circular fan prepared by the present invention can effectively inhibit the growth of mycelium of gray mold.
Example 3
Inhibition of Botrytis cinerea polysaccharide on germination of Botrytis cinerea spores
(1) Adding Padina macrophylla polysaccharide into PDA culture medium to obtain culture medium with concentration of 0,30,60,120mg/ml, pouring the culture into culture dish, and taking 0mg/ml as control group;
(2) Inoculating 10ul of spore suspension onto 9cm medicated PDA plate, spreading with coater, and culturing in 25 deg.C incubator for 48 hr;
(3) Detecting germination rate of more than 100 spores under a microscope, wherein the germination rate is used as a mark of germination according to the fact that the length of each spore bud exceeds half of the diameter length of each spore;
(4) Spore germination rate (%) =spore germination number/total number of detected spores x 100%,
spore germination inhibition (%) = (control germination rate-treatment germination rate/control spore germination rate) ×100%.
TABLE 2 Effect of Padina Botrytis polysaccharide on spore germination count
| Concentration of | Spore germination count | |
| 0 | 94.33±1.53 | - |
| 30 | 70.00±2.65 | 25.79% |
| 60 | 17.33±2.08 | 81.62% |
| 90 | 4.67±2.52 | 95.05% |
The experimental results of fig. 2 and table 2 show that the polysaccharide of sector algae prepared by the invention can effectively inhibit the spore germination number of gray mold.
Example 4
The invention provides a pesticide composition for preventing and treating gray mold, which is prepared from 10% of sector algae polysaccharide, 6% of fluxapyroxad, 4% of trifloxystrobin, 12% of N-methylpyrrolidone, 2% of sodium lignin sulfonate, 5% of sodium dodecyl sulfate and water to 100%. The gray mold can be better prevented and treated by the combination of the sector algae polysaccharide, the fluxapyroxad and the trifloxystrobin.
The technical features of the present invention that are not described in the present invention may be implemented by or using the prior art, and are not described in detail herein, but the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, but is also intended to be within the scope of the present invention by those skilled in the art.
Claims (2)
1. The application of the sector algae polysaccharide in preparing the gray mold treatment medicine is characterized in that the sector algae polysaccharide preparing method comprises the following steps:
(1) Placing clean and dried to constant weight of Sargassum horneri in pulverizer, pulverizing to obtain Sargassum horneri powder
(2) Placing the Padina pavonica powder into 30 times of purified water, and heating in water bath at 70deg.C for 5 hr to obtain Padina pavonica extract;
(3) Cooling to room temperature, and filtering the sector algae extracting solution to obtain sector algae filtrate;
(4) Concentrating the sector algae filtrate to 1/5 of the original volume, adding 1/3 volume of sevage reagent for deproteinization, and repeating for 5 times;
(5) According to 1:4, adding 95% ethanol in proportion, shaking uniformly, precipitating with ethanol for 24 hours, and centrifuging to obtain crude polysaccharide of the sector algae;
(6) And (3) dissolving the obtained crude polysaccharide of the sector algae, filtering by using an ultrafiltration membrane with a molecular weight cut-off of 100ku, collecting the cut-off liquid, and freeze-drying to obtain the polysaccharide of the sector algae.
2. The application of the sector algae polysaccharide in preparing the gray mold inhibitor is characterized in that the sector algae polysaccharide preparing method comprises the following steps:
(1) Placing the washed and dried large-area sector algae into a pulverizer, and pulverizing to obtain large-area sector algae powder;
(2) Placing the Padina pavonica powder into 30 times of purified water, and heating in water bath at 70deg.C for 5 hr to obtain Padina pavonica extract;
(3) Cooling to room temperature, and filtering the sector algae extracting solution to obtain sector algae filtrate;
(4) Concentrating the sector algae filtrate to 1/5 of the original volume, adding 1/3 volume of sevage reagent for deproteinization, and repeating for 5 times;
(5) According to 1:4, adding 95% ethanol in proportion, shaking uniformly, precipitating with ethanol for 24 hours, and centrifuging to obtain crude polysaccharide of the sector algae;
(6) And (3) dissolving the obtained crude polysaccharide of the sector algae, filtering by using an ultrafiltration membrane with a molecular weight cut-off of 100ku, collecting the cut-off liquid, and freeze-drying to obtain the polysaccharide of the sector algae.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211029822.3A CN115281194B (en) | 2022-08-26 | 2022-08-26 | Bactericide containing fluxapyroxad and trifloxystrobin |
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Citations (6)
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| CN101790985A (en) * | 2010-03-11 | 2010-08-04 | 浙江工业大学 | Use of cis-butenedioic anhydride compounds in preparation of botrytis resistant bactericides |
| CN105707072A (en) * | 2014-12-03 | 2016-06-29 | 中国科学院大连化学物理研究所 | Spirulina polysaccharide and application thereof |
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| CN111838166A (en) * | 2020-06-10 | 2020-10-30 | 青岛海大生物集团有限公司 | Preparation method and application of chlorella polysaccharide bactericide compound |
| CN111848827A (en) * | 2020-06-10 | 2020-10-30 | 青岛海大生物集团有限公司 | Novel chlorella polysaccharide bactericide and preparation method thereof |
| CN112175102A (en) * | 2020-10-27 | 2021-01-05 | 华南农业大学 | Preparation method and application of algal polysaccharide disease-resistant inducer |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2868253B1 (en) * | 2004-03-30 | 2006-07-21 | Cie Financiere Et De Participa | USE OF ULVANES AS ACTIVATORS OF PLANT DEFENSE REACTIONS AND RESISTANCE AGAINST BIOTIC OR ABIOTIC CONSTRAINTS |
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Patent Citations (6)
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| CN101790985A (en) * | 2010-03-11 | 2010-08-04 | 浙江工业大学 | Use of cis-butenedioic anhydride compounds in preparation of botrytis resistant bactericides |
| CN105707072A (en) * | 2014-12-03 | 2016-06-29 | 中国科学院大连化学物理研究所 | Spirulina polysaccharide and application thereof |
| CN106832030A (en) * | 2017-01-22 | 2017-06-13 | 嵊州市派特普科技开发有限公司 | The method that active polysaccharide is extracted from marine alga |
| CN111838166A (en) * | 2020-06-10 | 2020-10-30 | 青岛海大生物集团有限公司 | Preparation method and application of chlorella polysaccharide bactericide compound |
| CN111848827A (en) * | 2020-06-10 | 2020-10-30 | 青岛海大生物集团有限公司 | Novel chlorella polysaccharide bactericide and preparation method thereof |
| CN112175102A (en) * | 2020-10-27 | 2021-01-05 | 华南农业大学 | Preparation method and application of algal polysaccharide disease-resistant inducer |
Non-Patent Citations (2)
| Title |
|---|
| Moroccan seaweed polysaccharides elicit defense response and induce protection against Botrytis cinerea in tomato plants;MZIBRA A, AASFAR A, AZAROUAL S E, et al.;Moroccan Journal of Agricultural Sciences;第3卷(第1期);1-9 * |
| 海藻多糖的生理功能及其在动物生产上的应用;周华杰等;饲料与畜牧(第3期);23-26 * |
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