AU2021103028A4 - Method for preparing linalool microcapsule for controlling plant soil-borne disease - Google Patents
Method for preparing linalool microcapsule for controlling plant soil-borne disease Download PDFInfo
- Publication number
- AU2021103028A4 AU2021103028A4 AU2021103028A AU2021103028A AU2021103028A4 AU 2021103028 A4 AU2021103028 A4 AU 2021103028A4 AU 2021103028 A AU2021103028 A AU 2021103028A AU 2021103028 A AU2021103028 A AU 2021103028A AU 2021103028 A4 AU2021103028 A4 AU 2021103028A4
- Authority
- AU
- Australia
- Prior art keywords
- linalool
- microcapsule
- controlling plant
- plant soil
- borne diseases
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Ceased
Links
- CDOSHBSSFJOMGT-UHFFFAOYSA-N linalool Chemical compound CC(C)=CCCC(C)(O)C=C CDOSHBSSFJOMGT-UHFFFAOYSA-N 0.000 title claims abstract description 172
- 239000001490 (3R)-3,7-dimethylocta-1,6-dien-3-ol Substances 0.000 title claims abstract description 86
- CDOSHBSSFJOMGT-JTQLQIEISA-N (R)-linalool Natural products CC(C)=CCC[C@@](C)(O)C=C CDOSHBSSFJOMGT-JTQLQIEISA-N 0.000 title claims abstract description 86
- 229930007744 linalool Natural products 0.000 title claims abstract description 86
- 201000010099 disease Diseases 0.000 title claims abstract description 52
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 52
- 239000003094 microcapsule Substances 0.000 title claims abstract description 40
- 238000000034 method Methods 0.000 title claims description 17
- 238000002360 preparation method Methods 0.000 claims description 10
- 238000001291 vacuum drying Methods 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 229920000858 Cyclodextrin Polymers 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 4
- 239000002245 particle Substances 0.000 claims description 4
- 238000000227 grinding Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 28
- 239000000575 pesticide Substances 0.000 abstract description 21
- 239000003795 chemical substances by application Substances 0.000 abstract description 14
- 241000589771 Ralstonia solanacearum Species 0.000 abstract description 10
- 239000002689 soil Substances 0.000 abstract description 10
- 239000013043 chemical agent Substances 0.000 abstract description 3
- 230000000813 microbial effect Effects 0.000 abstract description 3
- 239000000447 pesticide residue Substances 0.000 abstract description 3
- 241000233645 Phytophthora nicotianae Species 0.000 abstract 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 32
- 241000196324 Embryophyta Species 0.000 description 27
- 241000208125 Nicotiana Species 0.000 description 27
- 239000003085 diluting agent Substances 0.000 description 20
- 230000001580 bacterial effect Effects 0.000 description 16
- 241000233647 Phytophthora nicotianae var. parasitica Species 0.000 description 15
- 238000003958 fumigation Methods 0.000 description 10
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 8
- 230000002401 inhibitory effect Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 244000061176 Nicotiana tabacum Species 0.000 description 5
- 238000002835 absorbance Methods 0.000 description 5
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 230000001018 virulence Effects 0.000 description 5
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000013641 positive control Substances 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 239000005802 Mancozeb Substances 0.000 description 3
- 239000005807 Metalaxyl Substances 0.000 description 3
- 229910052802 copper Inorganic materials 0.000 description 3
- 239000010949 copper Substances 0.000 description 3
- ZQEIXNIJLIKNTD-UHFFFAOYSA-N methyl N-(2,6-dimethylphenyl)-N-(methoxyacetyl)alaninate Chemical compound COCC(=O)N(C(C)C(=O)OC)C1=C(C)C=CC=C1C ZQEIXNIJLIKNTD-UHFFFAOYSA-N 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- -1 /or Species 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 2
- 244000105624 Arachis hypogaea Species 0.000 description 2
- 235000010777 Arachis hypogaea Nutrition 0.000 description 2
- 235000018262 Arachis monticola Nutrition 0.000 description 2
- 239000006137 Luria-Bertani broth Substances 0.000 description 2
- 241000031556 Phytophthora sp. Species 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000006285 cell suspension Substances 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000020232 peanut Nutrition 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- QNBTYORWCCMPQP-JXAWBTAJSA-N (Z)-dimethomorph Chemical compound C1=C(OC)C(OC)=CC=C1C(\C=1C=CC(Cl)=CC=1)=C/C(=O)N1CCOCC1 QNBTYORWCCMPQP-JXAWBTAJSA-N 0.000 description 1
- 240000005250 Chrysanthemum indicum Species 0.000 description 1
- 235000018959 Chrysanthemum indicum Nutrition 0.000 description 1
- JJLJMEJHUUYSSY-UHFFFAOYSA-L Copper hydroxide Chemical compound [OH-].[OH-].[Cu+2] JJLJMEJHUUYSSY-UHFFFAOYSA-L 0.000 description 1
- 239000005750 Copper hydroxide Substances 0.000 description 1
- 239000005751 Copper oxide Substances 0.000 description 1
- 239000005761 Dimethomorph Substances 0.000 description 1
- 239000005821 Propamocarb Substances 0.000 description 1
- 241000232299 Ralstonia Species 0.000 description 1
- 208000027418 Wounds and injury Diseases 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229910001956 copper hydroxide Inorganic materials 0.000 description 1
- 229910000431 copper oxide Inorganic materials 0.000 description 1
- 238000012272 crop production Methods 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 208000014674 injury Diseases 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 239000006877 oatmeal agar Substances 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- WZZLDXDUQPOXNW-UHFFFAOYSA-N propamocarb Chemical compound CCCOC(=O)NCCCN(C)C WZZLDXDUQPOXNW-UHFFFAOYSA-N 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000008223 sterile water Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
- A01N25/26—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests in coated particulate form
- A01N25/28—Microcapsules or nanocapsules
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
OF THE DISCLOSURE
The present disclosure provides use of linalool in controlling plant soil-borne diseases, and
relates to the technical field of pesticides. The present disclosure provides the use of linalool in
controlling plant soil-borne diseases, and prepares a linalool microcapsule for controlling plant soil
borne diseases. The agent has excellent control effects on soil-bome diseases caused by Phytophthora
nicotianae and Ralstonia solanacearum Ralstonia solanacearumand a better effect on fields where
both types of diseases occur in a mixed manner. Compared with chemical agents, the agent has
considerable efficacy, is environmentally friendly, and has no pesticide residue; the agent has superior
efficacy to microbial agents and stable efficacy.
Description
[01] The present disclosure belongs to the technical field of pesticides, and particularly relates to use of linalool in controlling plant soil-borne diseases.
[02] Soil-bome diseases seriously harm crop production; there are a variety of pathogens that have wide routes of transmission and are difficult to control. In particular, Phytophthorasp. and Ralstonia solanacearum can infect a plurality of crops and often lead to destructive diseases. Tobacco black shank and bacterial wilt are caused by these two pathogens, which are seriously harmful. Particularly, these two diseases occur in tobacco production areas of southern China in a mixed manner and are difficult to control. At present, control of these two diseases mainly depends on chemical agents. For example, control pesticides for tobacco black shank mainly include metalaxyl mancozeb, dimethomorph, propamocarb and the like; control pesticides for bacterial wilt mainly include copper agents such as thiediazole copper and copper hydroxide; these agents are very prone to pesticide residues, environmental pollution, and pesticide resistance of pathogens. Microbial pesticides deeply investigated so far have problems of poor control effect and unstable efficacy, which are difficult to replace chemical pesticides.
[03] In view of this, an objective of the present disclosure is to provide use of linalool in controlling plant soil-borne diseases; linalool has excellent antibacterial and control effects on tobacco black shank and bacterial wilt and particularly a better effect on tobacco fields where both types of diseases occur in a mixed manner compared with the existing agents.
[04] To achieve the above objective, the present disclosure provides the following technical solutions:
[05] The present disclosure provides use of linalool in controlling plant soil-borne diseases.
[06] Preferably, the plant soil-borne diseases may include plant soil-borne diseases caused by Ralstonia solanacearumand/or Phytophthora sp.
[07] The present disclosure further provides a method for preparing a linalool microcapsule for controlling plant soil-borne diseases, including the following steps: mixing linalool, p-cyclodextrin and water in a mass ratio of 1:8:8, stirring for 40 min, vacuum drying and grinding a resulting mixture, and collecting a portion with a particle size of <200 m to obtain the linalool microcapsule.
[08] Preferably, the vacuum drying may be conducted 70°C for 12 h.
[09] The present disclosure further provides a linalool microcapsule for controlling plant soil borne diseases prepared by the foregoing preparation method.
[10] Preferably, the content of linalool in the linalool microcapsule may be 80 5% by weight.
[11] The present disclosure provides use of the linalool microcapsule for controlling plant soil borne diseases prepared by the foregoing preparation method or the foregoing linalool microcapsule in controlling plant soil-borne diseases.
[12] Preferably, the plant may include one or more of tobacco, peanut, and/or vegetables.
[13] Preferably, the soil-borne diseases may include tobacco black shank and/or bacterial wilt.
[14] The present disclosure provides a method for controlling plant soil-borne diseases, where a diluent of the linalool microcapsule for controlling plant soil-borne diseases prepared by the foregoing preparation method or the foregoing linalool microcapsule is applied in fields.
[15] The present disclosure provides use of linalool in controlling plant soil-borne diseases, and prepares a linalool microcapsule for controlling plant soil-borne diseases. The agent has excellent control effects on soil-borne diseases caused by Phytophthoranicotianaeand R. solanacearumand a better effect on fields where both types of diseases occur in a mixed manner. Compared with chemical agents, the agent has considerable efficacy, is environmentally friendly, and has no pesticide residue; the agent has superior efficacy to microbial agents and stable efficacy.
[16] FIG. 1 illustrates a fumigation-based inhibitory effect of linalool on P nicotianae, where the left panel illustrates a fumigation-based inhibitory effect of 5 1 of linalool per Petri dish, and the right panel illustrates CK.
[17] The present disclosure provides use of linalool in controlling plant soil-borne diseases.
[18] The present disclosure has no particular limitation on the source of the linalool, and a commercially available reagent conventional in the art may be used. The soil-borne diseases of the present disclosure may preferably include plant soil-bome diseases caused by R. solanacearumand/or Phytophthora sp., more preferably tobacco black shank and bacterial wilt, and most preferably a mixed disease of tobacco black shank and bacterial wilt.
[19] The present disclosure further provides a method for preparing a linalool microcapsule for controlling plant soil-borne diseases, including the following steps: mixing linalool, p-cyclodextrin and water in a mass ratio of 1:8:8, stirring for 40 min, vacuum drying and grinding a resulting mixture, and collecting a portion with a particle size of <200 m to obtain the linalool microcapsule.
[20] In the present disclosure, the vacuum drying may preferably be conducted at 70°C for 12 h. The linalool microcapsule of the present disclosure uses linalool as an active ingredient and cyclodextrin as an embedding agent.
[21] The present disclosure further provides a linalool microcapsule for controlling plant soil borne diseases prepared by the foregoing preparation method.
[22] In the present disclosure, the content of linalool in the linalool microcapsule may preferably be 80 5% by weight.
[23] The present disclosure provides use of the linalool microcapsule for controlling plant soil borne diseases prepared by the foregoing preparation method or the foregoing linalool microcapsule in controlling plant soil-borne diseases. In the present disclosure, the plant may preferably include tobacco, peanut, and vegetables. In the present disclosure, the soil-borne diseases may preferably include tobacco black shank and/or bacterial wilt, and more preferably tobacco black shank caused by P nicotianae and tobacco bacterial wilt caused by R. solanacearum.
[24] The present disclosure provides a method for controlling plant soil-borne diseases, where a diluent of the linalool microcapsule for controlling plant soil-borne diseases prepared by the foregoing preparation method or the foregoing linalool is applied in fields.
[25] In the present disclosure, a 400- to 800-fold diluent of the linalool microcapsule may preferably be applied in the fields, 50 ml of the diluent may be applied to each plant, roots may be watered every seven days, and the application may be conducted twice in total.
[26] The use of linalool in controlling plant soil-borne diseases provided by the present disclosure will be described below in detail in connection with examples, but they should not be construed as limiting the protection scope of the present disclosure.
[27] Example 1
[28] Preparation of linalool microcapsule
[29] (1) Linalool, j-cyclodextrin and water were mixed in a mass ratio of 1:8:8, and fully stirred for 40 min;
[30] (2) the above sample was dried in a 70°C vacuum oven for 12 h; and
[31] (3) dried product was gently ground and sieved to obtain a portion with a particle size of 200 ptm, i.e., microcapsules, where linalool content was 80± 5%.
[32] Example 2
[33] Inhibitory effect of linalool on P nicotianae
[34] 1. Test methods
[35] 1.1 Plate antibacterial test for linalool
[36] Mycelium growth rate method. Linalool was dissolved in dimethylsulfoxide (DMSO) (0.5%, v/v) to prepare Oatmeal Agar Plates (OA, 30 g of oatmeal was boiled in 1,000 ml of water for 20 min, filtered through four layers of gauzes, mixed with 17 g of agar, and sterilized at 121°C for 20 min) at different concentrations; final concentrations of linalool were 5, 10, 20, 40, 80, and 120 pl/L. P nicotianaecakes (5 mm) were plated onto OA plates supplemented with different concentrations of Chrysanthemum indicum essential oil, cultured at 28°C for five days; using DMSO in the same dilution ratio as a control, colony diameters were measured by the cross method, and mycelial growth inhibition rate was calculated.
[37] 1.2 Measurement of fumigation effect
[381 The fumigation activity of linalool against P nicotianaewas measured by the sealed plate method. P nicotianaecakes with a diameter of 5 mm were plated onto OA plates supplemented with 0.625, 1.25, 2.5, 5, 10, 20, and 40pl (equivalent to 4.92, 9.84, 19.69, 39.37, 78.74, 157.48, and 314.96 ptl/L) of linalool, sealed with sealing film in triplicate, and cultured at 28°C for five days. Colony diameters were measured by the cross method, and IC5o was calculated; a plate without linalool was used as a control.
[39] Mycelial growth inhibition rate was calculated according to the following formula: (Control diameter - 5 mm) - (Treatment diameter - 5mm) Control diameter - 5 mm Inhibition rate (%)= X 100%,Formula L
[401 2. Results and analysis
[41] Measured by the mycelium growth rate method, a curve of virulence of linalool against P nicotianaeis y = 0.6431x + 3.5278 (R2 = 0.9935), where x is a logarithmic concentration, and y is a probability value; the IC5o is 18.03pl/L, indicating high antibacterial activity against P nicotianae. In addition, it is found that linalool has a better fumigation effect on P nicotianae, where the virulence curve is y = 2.7340 + 2.2765x (R2 = 0.9959), and ICo is 9.8941 (8.8932-11.0076). FIG. 1 illustrates that treatment with 5 pl of linalool per Petri dish can completely inhibit the growth of P nicotianae; therefore, linalool has excellent inhibitory and fumigation effects on P nicotianae.
[42] Example 3
[431 Inhibitory effect of linalool on R. solanacearum
[44] 1. Materials and methods
[45] The virulence of linalool against R. solanacearum was measured by the contact method. Preserved R. solanacearumwas activated on a Luria-Bertani (LB) agar (at 28°C); a single colony was picked into an LB broth, and cultured under shaking at 160 rpm and 28°C for 48 h; the concentration of the cell suspension was adjusted to 1 x 109 CFU/ml for use. Using the pesticide solution-cell suspension mixing method, linalool was dissolved in sterile water with 0.2% DMSO to formulate into a 400 t/L mother liquor, which was diluted to 200, 100, 50, 25, and 12.5 p/L, respectively. 5 ml each of diluents were charged into test tubes with 5 ml of LB broth, and 10pl of R. solanacearum suspension was charged thereinto; each concentration was repeated in triplicate. A culture medium without pesticide solution was used as a negative control; a culture medium without pesticide solution but with R. solanacearum was used as a positive control. All test tubes were subjected to shaking culture on a shaker (at 160 rpm and 28C) and removed until the absorbance (OD600) of the positive control was around 1.2; absorbance after treatment with each agent was measured spectrophotometrically; antibacterial effect and EC5 were calculated. Antibacterial effect (%) =
(Absorbance of positive control - Absorbance of treatment group)/Absorbance of positive control x 100, Formula II.
[46] 2. Results and analysis
[47] After measurement, a curve of virulence of linalool against R. solanacearum is y = 1.9377x + 2.5111, where x is a logarithmic concentration, and y is a probability value; the correlation coefficient is 0.9972, the EC5 is 19.2518 pl/L, and the 95% confidence interval ranges from 17.6625 to 20.9842. It is indicated that linalool has strong virulence against R. solanacearum and potential application value.
[48] Example 4
[49] Fumigation experiment for linalool in the control of tobacco black shank
[50] 1. Materials and methods
[51] Soil fumigation experiment: 1% and 2% diluents (diluted with 5% Tween 80 solution) of linalool microcapsule (prepared in Example 1) were prepared; sterile soil with P nicotianae-infected grains was potted (3 g/pot), and each pot contained 500 g of soil; 50 ml each of diluents of linalool microcapsule was poured into the pots; the pots were sealed with plastic film and fumigated for 5 days; at 5 days, the film was uncovered for sampling; after sampling, 50 ml each of diluents were poured into the pots again, sealed, fumigated for two days, and sampled again.
[52] Pot experiment: Tobacco seedlings of uniform size were transplanted in the fumigated soil and sampled at 5 days; the disease index was recorded at 15 days.
[53] 2. Results
[54] From Tables 1 and 2, the linalool microcapsule has a better control effect on tobacco black shank and a concentration effect, i.e., the inhibitory effect of the linalool microcapsule on P nicotianaeis enhanced as the concentration increases. The control effect of the linalool microcapsule is 82.61% 15 days after transplanting. Meanwhile, 2% addition leads to pesticide injury, and 1% addition shows a better control effect.
[55] Table 1 The copy number of DNA fragments of P nicotianae in the fumigated soil Treat Fumigation for 5 days Fumigation for 7 days Potting for 5 days ment
CK 11,827,274.97 247,720.52a 12,527,258.76 980,953.52a 11,796,836.01 392,644.87a
1% 5,517,261.79 76,709.39b 602,992.56 10,953.22b 614,390.14 29,411.72b
2% 603,049.13 9,405.51c 72,389.78 4,869.53b 128,774.24 10,354.35b
[56] Table 2 The control effect of linalool microcapsule on tobacco black shank (at 15 days) Treatment Disease index Control effect(%)
1% 12.18 82.61
CK 70.05
[57] Example 5
[58] Pot experiment for linalool in the control of tobacco bacterial wilt
[59] The linalool microcapsule prepared in Example 1 was diluted 400-, 600- and 800-fold, and poured into roots of healthy potted tobacco seedlings at the 5-to-6-leaf stage; 20 ml of each diluent was poured per seedling; the procedure was repeated in triplicate every 15 seedlings were treated. After the seedlings were treated with the agent for 24 h, several holes were punched around the caudex using a sterile needle, and 108c fu/ml R. solanacearum suspension was poured along the caudex, 20 ml per seedling. The seedlings were cultivated in a phytotron at 28°C; once the control incidence was more than 90%, the number of diseased seedlings was recorded and the corrected efficacy was calculated.
[60] Corrected efficacy (%) = (Treated incidence - control incidence)/Control incidence x 100, Formula III.
[61] The control effect is shown in Table 3. The 400-, 600- and 800-fold diluents of linalool microcapsule have some control effects on tobacco bacterial wilt, and particularly, the 400-fold diluent has the optimal control effect, which reaches up to 69.05%.
[62] Table 3 Control effect of linalool on tobacco bacterial wilt (12 days) Treatment Incidence (%) Control effect (%)
400-fold diluent of linalool 28.89 3.85 69.05
600-fold diluent of linalool 42.22 3.85 54.76
800-fold diluent of linalool 53.33 6.67 42.86
CK 93.33 6.67
[63] Example 6
[64] Control effect of linalool on fields suffering from both tobacco bacterial wilt and tobacco black shank
[65] The test was conducted in the Zheng'an tobacco field, Zunyi in 2017. Tobacco black shank and bacterial wilt were occurred in a mixed manner in experimental fields. The tobacco cultivar planted was K326. Test pesticides were a 400-fold diluent of linalool microcapsule prepared in the example, a 400-fold diluent of 58% metalaxyl mancozeb (a major pesticide for controlling tobacco black shank), and a 400-fold diluent of 20% thiediazole copper (a major pesticide for controlling bacterial wilt), respectively. Plots were arranged randomly, area of each plot was 30 m2 , and tests were done in triplicate. Pesticide application began once tobacco plants had symptoms in the fields; each pesticide was applied twice every seven days; roots was watered and 50 ml of the pesticide was applied to each plant.
[66] The incidence was investigated 10 days after the second pesticide application, and disease index and control effect were calculated. Results are shown in Table 4. Because both tobacco black shank and bacterial wilt occurred in the tobacco field and the control effect of each major pesticide for a single disease was undesired in production, the linalool microcapsule had a significantly better effect than both pesticides and exhibited an advantage of controlling both diseases. However, as the diseases were aggravated at a later stage, none of pesticides could effectively control disease development, but linalool microcapsule-treated tobacco plants exhibited milder disease severity than those treated with others.
[67] Table 4 Control effect of linalool on tobacco bacterial wilt (12 days) Treatment Incidence (%) Control effect (%)
400-fold diluent of linalool 8.37 1.17d 61.50a
400-fold diluent of 58% 48.10b 12.32 1.39c metalaxyl mancozeb
400-fold diluent of 20% 37.95c 15.32 1.82b thiediazole copper
CK 29.53 3.39a
[68] The foregoing descriptions are only preferred implementations of the present disclosure. It should be noted that several improvements and modifications may further be made by a person of ordinary skill in the art without departing from the principle of the present disclosure, and such improvements and modifications should also be deemed as falling within the protection scope of the present disclosure.
Claims (5)
1. A method for preparing a linalool microcapsule for controlling plant soil-borne diseases, comprising the following steps: mixing linalool, p-cyclodextrin and water in a mass ratio of 1:8:8, stirring for 40 min, vacuum drying and grinding a resulting mixture, and collecting a portion with a particle size of <200 pm to obtain the linalool microcapsule.
2. The preparation method according to claim 1, wherein the vacuum drying is conducted at 70°C for 12 h.
3. A linalool microcapsule for controlling plant soil-borne diseases prepared by the preparation method according to claim 1 or 2.
4. The linalool microcapsule according to claim 3, wherein the content of linalool in the linalool microcapsule is 80 5% by weight.
5. Use of the linalool microcapsule for controlling plant soil-borne diseases prepared by the preparation method according to claim 1 or 2 in controlling plant soil-borne diseases.
1 /1
FIG. 1
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2021103028A AU2021103028A4 (en) | 2021-06-02 | 2021-06-02 | Method for preparing linalool microcapsule for controlling plant soil-borne disease |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AU2021103028A AU2021103028A4 (en) | 2021-06-02 | 2021-06-02 | Method for preparing linalool microcapsule for controlling plant soil-borne disease |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AU2021103028A4 true AU2021103028A4 (en) | 2021-07-29 |
Family
ID=76972133
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AU2021103028A Ceased AU2021103028A4 (en) | 2021-06-02 | 2021-06-02 | Method for preparing linalool microcapsule for controlling plant soil-borne disease |
Country Status (1)
| Country | Link |
|---|---|
| AU (1) | AU2021103028A4 (en) |
-
2021
- 2021-06-02 AU AU2021103028A patent/AU2021103028A4/en not_active Ceased
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN112899205B (en) | Pseudomonas chlororaphis MN225969 and application thereof | |
| CN1932006A (en) | Prepn process of strain and prepn for controlling blight of vegetable biologically | |
| CN101054565A (en) | Biological control strain capable of preventing and curing root knot nematode disease for greenhouse vegetable | |
| NO316012B1 (en) | Pseudomonas strain, as well as composition and method of controlling plant diseases | |
| CN116622581B (en) | Bacillus bailii HM-3 and application thereof | |
| HU214457B (en) | Method for screening antifungal activity, compositions containing antifungal microorganisms screened with this method, and method for inhibiting plant fungal diseases | |
| HU220838B1 (en) | Microorganisms for biological control of plant diseases | |
| CN116391726B (en) | Compound biocontrol microbial inoculum for preventing and treating bacterial canker of kiwi fruits | |
| CN112889843A (en) | Killed rice-germ bacillus wettable powder for preventing and treating citrus red spiders and application thereof | |
| CN102524303A (en) | Mixture for preventing blight and verticillium wilt of cotton | |
| AU2021103028A4 (en) | Method for preparing linalool microcapsule for controlling plant soil-borne disease | |
| CN114634878B (en) | Composite microbial preparation and application thereof in preventing and controlling root knot nematode | |
| KR20220090446A (en) | Novel bacteriophage effective for fire blight bacteria and black shoot blight disease bacteria, and use thereof | |
| CN113207896B (en) | Application of linalool in preventing and treating plant soil-borne diseases | |
| CN111995472A (en) | Disease-resistant growth-promoting type composite slow-release fertilizer and preparation method thereof | |
| CN114747595B (en) | Application of Burkholderia cepacia JT79 in controlling meloidogyne incognita | |
| CN112553085B (en) | Nontoxic rice blast strain for preventing and controlling rice blast and application thereof | |
| CN114891679B (en) | Pseudomonas fluorescens and application thereof in preventing and controlling cherry branch diseases | |
| CN110583705B (en) | Application of ragweed essential oil as verticillium dahliae bactericide | |
| CN116333891B (en) | Biocontrol bacterium JSNL-TX55 for gray mold and anthracnose of strawberries and application thereof | |
| CN113502227B (en) | Fusarium vine, microbial inoculum and herbicide containing same and application of Fusarium vine | |
| CN111876337B (en) | Fusarium oxysporum and fermentation method and application thereof | |
| CN109082389B (en) | Soil streptomycete with biological fertilizer and biological pesticide potentials | |
| CN115261278A (en) | Novel application of bacillus pumilus LYMC-3 and fermentation liquor thereof and preparation method of fermentation liquor thereof | |
| نبيل جاسم اليوسف | EVALUATING THE EFFECTIVENESS OF SOME ORGANIC FERTILIZERS, BIO-PREPARATIONS AND THEIR COMBINATIONS TO RESIST FUSARIUM WILT DISEASE ON CUCUMBER |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FGI | Letters patent sealed or granted (innovation patent) | ||
| MK22 | Patent ceased section 143a(d), or expired - non payment of renewal fee or expiry |