CN110583705B - Application of ragweed essential oil as verticillium dahliae bactericide - Google Patents
Application of ragweed essential oil as verticillium dahliae bactericide Download PDFInfo
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Abstract
The invention relates to an application of ragweed essential oil as verticillium dahliae bactericide, which adopts two methods of contact and fumigation of ragweed essential oil to inhibit the growth of verticillium dahliae, and the result shows that: the ragweed essential oil has obvious influence on the growth of the hypha of the verticillium dahliae at a low concentration of 0.5 mu l/ml, and the growth inhibition effect is gradually enhanced along with the increase of the concentration; when the concentration is continuously increased to 5 mul/ml, the hypha of the inoculated fungus cake completely loses the activity of continuous expansion, and the growth of the hypha is completely inhibited; in a fumigation experiment, ragweed essential oil fumigates verticillium dahliae fungus cakes at the concentration of 0.2 mul/ml, and hypha of the fungus cakes are obviously inhibited; when the concentration reaches 1.2 mul/ml, the hyphae are completely killed and lose the activity; the ragweed essential oil provided by the invention can inhibit the growth of verticillium dahliae, is remarkably originated from plants, can be decomposed in nature, is environment-friendly and does not pollute the environment, and can be used for carrying out waste utilization on harmful plants.
Description
Technical Field
The invention relates to application of overground part essential oil extracted from an exotic invasive plant ragweed plant as a verticillium dahliae bactericide.
Background
The chemical synthetic bactericide is applied to agriculture, forestry and animal husbandry production, and is difficult to decompose in nature so as to be retained for a long time, so that the problems of environmental toxicity and phytotoxicity are increasingly serious. However, natural bactericidal active substances extracted from nature have incomparable advantages of synthetic bactericides such as easy degradation, no residue, low toxicity and the like, and the research of the natural bactericidal active substances is increasingly paid attention to by people. In addition, although some chemical fungicides are decomposed in soil, pathogenic bacteria gradually develop resistance to the fungicides, resulting in reduction or even loss of efficacy, since they are applied in large quantities for a long period of time. Under the circumstances, the research and development of the bactericide with novel action targets and action mechanisms are particularly important. Natural products with rich varieties, different configurations and various action mechanisms exist in the nature, and the possibility is provided for developing natural bactericides. According to investigation, more than 8 thousands of pathogenic microorganisms harmful to plants exist in the world. Plant diseases cause great losses to agriculture, whereby crop plants worldwide reduce the yield by about 500Mt per year on average. The verticillium dahliae is the main disease of cotton verticillium wilt, has a wide host range, and can be used for killing 660 plants of 38 families reported abroad, wherein 184 crops and 153 weeds are planted. According to the identification of China, at least 20 host plants are 80, and field crops comprise sunflower, eggplant, peppers, tomatoes, tobacco, potatoes, melons, watermelons, cucumbers, peanuts, kidney beans, mung beans, soybeans, sesame, beet and the like. Therefore, it is necessary to develop a natural and highly efficient bactericide for verticillium dahliae.
Ragweed (Ambrosia artemisiifolia L.) is a plant of the genus Ambrosia of the family Compositae, an annual weed, an invasive plant widely distributed worldwide and extremely harmful. After the 20 th century and the 30 th century are abroad transferred to the northeast three provinces of China, the world-wide harmful grass spreads nationwide, causing great harm to the natural environment and the production of agriculture and animal husbandry, and the documents 1 to 3 refer to.
Ragweed essential oil is reported to have contact poisoning activity on field pests and can inhibit microbial growth, but the inhibition effect of ragweed essential oil on the pathogen of the verticillium dahliae is not reported, and the report is shown in documents 4 to 8. Ragweed is fast in growth and strong in vitality, seriously harms agriculture, forestry and animal husbandry production of invaded land and stabilizes an ecosystem (see documents 9 to 10). The plant is used for extracting the essential oil, so that the essential oil is effectively utilized, and the spreading and expansion of the essential oil can be effectively controlled.
Reference documents:
wang Rui, Zhou Zhong Cheng, Zhangliang, Guo Jianying, Gaoshan Bin, Sun Yu Fang, Pawai Dong, Zhang Paoduo, Wanfang Hao.
Quzhi Ambrosia artemisiifolia main hazard and control method [ J ]. New agriculture, 2016, (14),39.
Dongli, Songzhili, the invasion speed, harm and prevention and treatment strategy of Ili valley ragweed and Sanqiao ragweed [ J ] Sinkiang agricultural science, 2017, (05),45-46.
Vidotto F,Tesio F,Ferrero A.Allelopathic effects of Ambrosia artemisiifolia L.in the invasive process[J].Crop Protection,2013,54(12),161-167.
Brückner,D.J.The allelopathic effect of ragweed(Ambrosia artemisiifolia L.)on the germination of cultivated plants[J].Novenytermeles,1998,47(6),635-644.
GC and GC/MS analysis of the volatile constituents of Ambrosia grandis and Ambrosia trifida [ J ] Mass Spectroscopy, 1996,17(3),37-41.
Chalchat,J.C.,Maksimovic,Z.A.,Petrovic,S.D.,Gorunovic,M.S.,Mraovic,M.Chemical composition and antimicrobial activity of ambrosia artemisiifolia l.essential oil[J].Journal of Essential Oil Research,2004,16(3),270-273.
Xianxinging, yinhao. ragweed has biochemical allelopathy to several economic crops [ J ]. Hainan university Committee (Nature science edition), 2003,21(1),69-73.
Major hazard and prevention and control measures of bang grass in liuyuchun [ J ]. hunan agricultural science, 2012, (24),75-75.
The hazard and control of ambrosia artemisiifolia is [ J ] Guangdong agricultural science, 2009, (10), 110-.
Disclosure of Invention
The invention aims to provide application of the ragweed essential oil as the verticillium dahliae bactericide, and the growth of the verticillium dahliae is inhibited by adopting two methods of contact and fumigation of the ragweed essential oil, and the result shows that: the ragweed essential oil has obvious influence on the growth of the hypha of the verticillium dahliae at a low concentration of 0.5 mu l/ml, and the growth inhibition effect is gradually enhanced along with the increase of the concentration; when the concentration is continuously increased to 5 mul/ml, the hypha of the inoculated fungus cake completely loses the activity of continuous expansion, and the growth of the hypha is completely inhibited; in a fumigation experiment, ragweed essential oil fumigates verticillium dahliae stipes at the concentration of 0.2 mul/ml, and hypha of a fungus cake is obviously inhibited; when the concentration reaches 1.2 mul/ml, the hyphae are completely killed and lose the activity; compared with the two methods of contact and fumigation, the sterilizing effect of fumigating verticillium dahliae by the ragweed essential oil is the best. The bacteriostatic activity of the ragweed essential oil on verticillium dahliae is not reported at home and abroad at present.
The invention relates to an application of ambrosia artemisiifolia essential oil as a verticillium dahliae bactericide, which is characterized in that the ambrosia artemisiifolia essential oil is dissolved by 0.1% Tween20 aqueous solution, diluted by 20-100 times and sprayed, contacted or fumigated by verticillium dahliae, wherein the concentration of the solution is 10-50 mu l/ml.
The invention discloses application of ragweed essential oil as a verticillium dahliae bactericide, wherein the main chemical components of the ragweed essential oil are shown in a table 1;
ragweed essential oil shown in table 1 has 35 chemical components, accounting for 96.12% of the total essential oil components, representing the main chemical components exerting bactericidal activity;
TABLE 1 essential chemical composition of Ambrosia artemisiifolia essential oil
The invention relates to an application of ragweed essential oil as a Verticillium dahliae bactericide, wherein a Verticillium dahliae (Verticillium dahliae) strain is a deciduous strong pathogenic strain V991 and is a strain preserved by Xinjiang institute of ecology and geography of the Chinese academy of sciences.
The invention relates to application of ragweed essential oil as verticillium dahliae bactericide, wherein the ragweed essential oil is obtained from the overground part of a ragweed plant by a steam distillation method, and the essential oil components of the ragweed essential oil naturally exist in the ragweed plant and can be decomposed in the nature, so that the environment is not polluted, the collection time is 2019, the collection place is 8 months: xinyuan county, Ili, Uygur autonomous region, Xinjiang.
The ragweed essential oil can be used as a common bactericide, can be prepared into different concentrations to fumigate seeds and soil, or can be mixed with other pesticides for use in the verticillium wilt occurrence period caused by the verticillium dahliae, is dissolved by Tween20 aqueous solution with the volume ratio of 0.1 percent and then is diluted by 20 to 100 times before use, and the spraying contact and fumigation concentration is 10 to 50 mu l/ml.
The application of the ragweed essential oil as the verticillium dahliae bactericide can achieve the following beneficial effects by implementation:
1. the ragweed essential oil provided by the invention can obviously inhibit the growth of verticillium dahliae hyphae under the condition of spraying contact and at a low concentration of 0.5 mu l/ml, the growth inhibition effect is gradually enhanced along with the increase of the concentration, and the verticillium dahliae hyphae can be completely killed when the concentration is increased to 5 mu l/ml; when the verticillium dahliae is fumigated, the ragweed essential oil suspension with the concentration of 0.2 mul/ml can obviously inhibit the growth of the verticillium dahliae hyphae, and the fumigation effect is better along with the increase of the concentration; when the concentration reaches 1.2. mu.l/ml, the verticillium dahliae hyphae completely lose activity.
2. The ragweed essential oil is suitable for preventing and removing verticillium wilt caused by verticillium dahliae in the farmland; the essential oil is synthesized into natural products, can be rapidly decomposed in nature, and does not pollute the environment; the ragweed essential oil is extracted from an exotic invasive harmful plant ragweed, the invasive plant has luxuriant growth in the field, strong viability, rapid expansion and high biomass, and the bactericide extracted from the invasive plant is beneficial to environmental protection, and comprehensively utilizes the harmful plant, thereby killing two birds with one stone. The ragweed essential oil is used as the verticillium dahliae bactericide, is derived from natural plants, can be decomposed in nature, is environment-friendly and does not pollute the environment, and simultaneously makes waste utilization on harmful plants.
Drawings
FIG. 1 is a diagram showing the measurement of the diameter of a colony of the ambrosia artemisiifolia essential oil influencing the growth contact of the stipe of Verticillium dahliae, wherein the action concentrations are respectively 0,0.5,1.5,2,2.5,3,3.5,4 and 5 mul/ml;
FIG. 2 shows the contact effect of ragweed essential oil of different concentrations on the growth of the hyphae of Verticillium dahliae, wherein the concentrations are 0,0.2,0.4,0.6,0.8,1, 1.2. mu.l/ml respectively;
FIG. 3 is a diagram showing the measurement of the diameter of a colony of the influence of the fumigation of the ragweed essential oil on the growth of the hyphae of the Verticillium dahliae cake, wherein the action concentrations are 0,0.2,0.4,0.6,0.8,1 and 1.2. mu.l/ml respectively;
FIG. 4 is a graph showing the fumigation effect of ragweed essential oil of different concentrations on the growth of verticillium dahliae hyphae, wherein the action concentrations are 0.2,0.4,0.6,0.8,1, 1.2. mu.l/ml respectively.
Detailed Description
The present invention will be described below by way of examples, but the present invention is not limited to the following examples.
All materials, reagents and equipment selected for use in the present invention are well known in the art, and other reagents and equipment well known in the art may be adapted for use in the practice of the present invention in the following examples.
Example 1
The spraying contact influence of the ragweed essential oil on the verticillium dahliae hyphae:
cutting the overground part of a ragweed plant collected in the field into small sections, putting the small sections into an essential oil distillation extractor, distilling for 3-6 hours to obtain ragweed essential oil, respectively adding 5, 10, 15, 20, 25, 30, 35, 40 and 50 mu l of the essential oil into 1ml of Tween20 aqueous solution with the concentration of 0.1 percent to dissolve the essential oil to prepare essential oil suspension with different concentrations, then respectively mixing the essential oil suspension with 9ml of PDA (potato culture medium) uniformly, pouring the mixture into a flat dish to ensure that the concentration of the ragweed essential oil in the flat dish is respectively 0.5 mu l/ml, 1 mu l/ml, 1.5 mu l/ml, 2 mu l/ml, 2.5 mu l/ml, 3 mu l/ml, 3.5 mu l/ml, 4 mu l/ml and 5 mu l/ml; placing the Verticillium dahliae cake with diameter of 5mm cultured for 7 days on ragweed essential oil with different concentrations, sealing with sealing film, and using 0.1% Tween20 water solution as blank control; repeating the treatment for 3 times, culturing in 25 deg.C incubator for 7 days, and observing the growth condition of mycelia;
the data statistical method comprises the following steps: detecting whether the difference among each group of data is significant (p is less than 0.05) by using the single-factor variance, and obtaining the average value and the standard deviation;
as a result: at a low concentration of 0.5. mu.l/ml, the growth of Verticillium dahliae hyphae was significantly affected, and the degree of the effect was gradually increased as the concentration was increased. When the concentration was increased to 5. mu.l/ml, the hyphae were completely killed, and the effect is shown in FIGS. 1 and 2.
Example 2
Fumigation effect of ragweed essential oil on the growth of verticillium dahliae hyphae:
the method comprises the following steps: selecting a glass plate with the diameter of 85mm, pouring 20ml of PDA culture medium, reserving 50ml of air in the plate, dissolving ragweed essential oil by using Tween20 aqueous solution with the concentration of 0.1%, respectively diluting to prepare essential oil suspension with the concentration of 10, 20, 30, 40, 50 and 60 mul/ml, respectively sucking 1ml of essential oil suspension with different concentrations, dripping the essential oil suspension on a sterilized filter paper sheet with the thickness of 70mm, putting the filter paper sheet on the inner surface of a plate cover, finally enabling the concentration of the essential oil/air in the plate to be 0.2,0.4,0.6,0.8 and 1.2 mul/ml, inoculating 7-day-cultured verticillium dahliae cake with the diameter of 5mm on the PDA culture medium, sealing the plate by using a sealing film, culturing in an incubator at the temperature of 25 ℃, using 0.1% Tween20 aqueous solution as blank contrast, repeating each concentration for 3 times, fumigating the ragweed essential oil by volatilization, observing the size of a stipe flora ring after 7 days of culture, and measuring the diameter of the stipe flora ring (the diameter of the stipe is measured inside);
as a result: when the concentration is 0.2 mul/ml (essential oil/air in dish), the growth of verticillium dahliae hyphae is obviously inhibited; when the concentration reaches 1.2. mu.l/ml, the hyphae lose activity completely, and the specific effect is shown in FIG. 3 and FIG. 4.
And (4) conclusion:
the application of the ragweed essential oil as the verticillium dahliae bactericide provided by the invention is proved by tests to comprise the following steps: when the ragweed essential oil contacts the verticillium dahliae hyphae at a low concentration of 0.5 mu l/ml, the growth of the hyphae can be obviously inhibited; when the using concentration reaches 5 mul/ml, hyphae are killed and lose activity completely; when the verticillium dahliae is fumigated by the ragweed essential oil with the concentration of 0.2 mul/ml, the growth of hyphae is also obviously inhibited; the sterilizing effect is better along with the increase of the concentration; when the concentration reaches 1.2 mul/ml, the verticillium dahliae hyphae completely lose activity, and the effect of contacting the concentration of 5 mul/ml is achieved; therefore, the fumigation of the ragweed essential oil has stronger killing power to verticillium dahliae than the spraying contact, the ragweed essential oil is extracted from the overground part of a ragweed plant, is derived from the plant, can be decomposed in the nature, is beneficial to environmental protection, and comprehensively utilizes harmful plants.
Claims (1)
1. An application of ambrosia artemisiifolia essential oil as a verticillium dahliae bactericide is characterized in that the ambrosia artemisiifolia essential oil is dissolved by a Tween20 aqueous solution with the concentration of 0.1%, diluted by 20-100 times and sprayed and contacted or fumigated with 10-50 mu l/ml.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| DE202007015735U1 (en) * | 2007-11-12 | 2008-01-03 | Öktem, Alper, Dr.med. | Preparations as pharmaceuticals, disinfectants, care and hygiene preparations or wound therapeutics |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE202007015735U1 (en) * | 2007-11-12 | 2008-01-03 | Öktem, Alper, Dr.med. | Preparations as pharmaceuticals, disinfectants, care and hygiene preparations or wound therapeutics |
Non-Patent Citations (3)
| Title |
|---|
| "Chemical Composition and Antimicrobial Activity of Ambrosia artemisiifolia L. Essential Oil";Jean-Claude Chalchat 等;《Journal of Essential Oil Research》;20040501(第16期);第271-272页，表I和II * |
| "Production of anti-fungal volatiles by non-pathogenic Fusarium oxysporum and its efficacy in suppression of Verticillium wilt of cotton";Qinghua Zhang 等;《Plant and Soil》;20150321(第392期);摘要，第106页表2 * |
| "豚草精油的提取及其生物活性的研究";王娟;《中国优秀硕士学位论文全文数据库 工程科技I辑》;20120415;第2.2.2和3.2小节 * |
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