CN115263040A - Nucleic acid amplification detection laboratory - Google Patents
Nucleic acid amplification detection laboratory Download PDFInfo
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- CN115263040A CN115263040A CN202211083568.5A CN202211083568A CN115263040A CN 115263040 A CN115263040 A CN 115263040A CN 202211083568 A CN202211083568 A CN 202211083568A CN 115263040 A CN115263040 A CN 115263040A
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- 230000003321 amplification Effects 0.000 title claims abstract description 54
- 238000003199 nucleic acid amplification method Methods 0.000 title claims abstract description 54
- 238000001514 detection method Methods 0.000 title claims abstract description 20
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 20
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 20
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 claims abstract description 114
- 239000002906 medical waste Substances 0.000 claims abstract description 65
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 42
- 239000000463 material Substances 0.000 claims abstract description 34
- 238000000034 method Methods 0.000 claims abstract description 4
- 238000012546 transfer Methods 0.000 claims description 105
- 238000004458 analytical method Methods 0.000 claims description 33
- 238000002474 experimental method Methods 0.000 claims description 23
- 208000015181 infectious disease Diseases 0.000 abstract description 7
- 230000001681 protective effect Effects 0.000 abstract description 6
- 238000012864 cross contamination Methods 0.000 abstract description 4
- 230000008569 process Effects 0.000 abstract description 3
- 239000000523 sample Substances 0.000 description 71
- 230000000694 effects Effects 0.000 description 4
- 241000700605 Viruses Species 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003139 buffering effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000006101 laboratory sample Substances 0.000 description 1
- 239000004816 latex Substances 0.000 description 1
- 229920000126 latex Polymers 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000007726 management method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000005180 public health Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
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- E—FIXED CONSTRUCTIONS
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- E04H—BUILDINGS OR LIKE STRUCTURES FOR PARTICULAR PURPOSES; SWIMMING OR SPLASH BATHS OR POOLS; MASTS; FENCING; TENTS OR CANOPIES, IN GENERAL
- E04H3/00—Buildings or groups of buildings for public or similar purposes; Institutions, e.g. infirmaries or prisons
- E04H3/08—Hospitals, infirmaries, or the like; Schools; Prisons
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Abstract
The invention discloses a nucleic acid amplification detection laboratory capable of being developed and having an infection risk, which is provided with a plurality of independent special channels for personnel, samples, materials, medical wastes, preparation reagents, three-level protective equipment medical wastes and the like, provides convenient, reasonable, practical and efficient laboratory biological safety management conditions, and avoids multiple cross contamination and biological potential safety hazards to laboratory areas, personnel, materials and samples in the laboratory operation process.
Description
Technical Field
The invention belongs to the field of nucleic acid amplification detection laboratories, and particularly relates to a laboratory capable of carrying out nucleic acid amplification detection with infection risk.
Background
The biological safety problem of the current nucleic acid amplification detection laboratory is raised to an unprecedented level of attention, and becomes one of the major public health problems facing the world; laboratory nucleic acid amplification detection is an important standard for population screening, clinical diagnosis and monitoring of treatment effects, and biological safety of a nucleic acid amplification detection laboratory is important.
Laboratories with infection risk, such as virus nucleic acid amplification detection, etc., should be provided with at least three experimental zones, namely a reagent storage and preparation zone, a specimen preparation zone, and an amplification and product analysis zone, and should be completely independent in physical space; the reagent storage and preparation area, the specimen preparation area and the amplification and product analysis area are provided with independent buffer rooms or air lock chambers to ensure the stability of the space pressure in the three experimental areas; the reagent storage and preparation area needs to be provided with a special one-way channel for transferring the configured reagent to the sample preparation area, the sample preparation area needs to be provided with a special one-way channel for transferring the prepared sample to the amplification and product analysis area, and the reagent storage and preparation area, the sample preparation area and the amplification and product analysis area need to be respectively provided with a special one-way channel for transferring the medical wastes of each experimental area to the medical waste disposal area; limited to the above requirements and the available space limitations of the laboratory, the laboratory will be provided with a personnel channel and a logistics channel, referred to as dual channels for short, respectively; however, when detection with infection risk such as virus nucleic acid amplification detection is performed, double channels are arranged in the passing and transferring processes of laboratory personnel, samples, materials and medical wastes, and various cross contamination and biological potential safety hazards appear.
Under the condition of double-channel arrangement, materials such as reagents, consumables and the like are transported through personnel channels, and personnel in each experimental area enter a laboratory from each area buffer room, and particularly when the materials are transported to enter a laboratory specimen preparation area, if no special channel exists, great hospital risk is caused; under the binary channels sets up the condition, the sample is transported and is passed through personnel's passageway, is brought into the laboratory from between the buffering of specimen preparation district by the experimenter, very easily causes to pollute personnel's passageway and use the current other personnel of this passageway to infect the risk.
The laboratory sample preparation area relates to factors such as unpacking of a sample transfer box, oscillation of a sample acquisition tube, uncovering, sample preparation, overflow and exposure of samples in the transfer box and the like, and is managed and operated according to the protection requirements of a biological safety three-level laboratory; experimenters need to wear disposable three-level protective equipment such as waterproof isolation clothes, conjoined protective clothes, N95 masks, medical protective caps, double-layer latex gloves, waterproof shoe covers and the like to carry out related experimental operation; when the experimenter leaves the experimental area, the related detached protective equipment is transported as medical waste; under the condition that the laboratory is provided with double channels, laboratory personnel in the region can be detached and packaged in the buffer room and are taken away from the nucleic acid amplification detection laboratory by the personnel channel, so that the personnel channel is extremely easy to be polluted and other personnel who use the channel to pass are easily infected.
Disclosure of Invention
The invention aims to provide a laboratory capable of developing nucleic acid amplification detection with infection risk, which solves the problems in the prior art, and avoids cross contamination of the internal space of the laboratory by arranging a plurality of independent transfer channels such as personnel, samples, materials, medical wastes, preparation reagents and the like, thereby ensuring the biological safety and the standard development of nucleic acid amplification detection with infection risk in the laboratory for nucleic acid amplification detection.
In order to achieve the purpose, the invention provides the following scheme: the invention provides a nucleic acid amplification detection laboratory, which consists of a one-way personnel passage channel, a material transfer channel, a sample transfer channel, a medical waste transfer channel, a reagent storage and preparation area, a specimen preparation area and an amplification and product analysis area; the reagent storage and preparation area, the specimen preparation area, the amplification and product analysis area are completely independent in physical space, and are respectively provided with a buffer room connected with a one-way passage channel for personnel.
The reagent storage and preparation area experiment area and the specimen preparation area are respectively provided with a material transfer cabinet connected with a material transfer channel; the sample preparation area experimental area is provided with a sample transfer cabinet connected with a sample transfer channel; a closed gallery is arranged above the material transfer cabinet and the sample transfer cabinet in the sample preparation area, and a stair is arranged on the side surface of the sample transfer cabinet and is separated from the material transfer cabinet, the sample transfer cabinet and the medical waste transfer channel in the sample preparation area in space; the reagent storage and preparation area medical waste transfer window is positioned above the gallery and is connected with the medical waste transfer channel; the reagent storage and preparation area experiment area is provided with a preparation reagent transfer window connected with the specimen preparation area.
The specimen preparation area experimental area is provided with a medical waste transfer cabinet connected with a medical waste transfer channel; the sample preparation area experimental area is provided with a sample transfer window which is connected with the amplification and product analysis area experimental area; the sample preparation area buffer room is arranged between the one-way personnel passing channel and the medical waste transferring channel, the buffer room is connected with the one-way personnel passing channel, and the medical waste transferring cabinet is arranged in the buffer room and connected with the medical waste transferring channel; a closed experiment table is arranged between the medical waste transfer cabinet in the sample preparation area buffer room and the sample preparation area experiment area and is separated from the medical waste transfer cabinet and the sample preparation area experiment area space; the sample transfer window of the amplification and product analysis area is positioned above the experiment table and is connected with the experiment area of the amplification and product analysis area.
And the amplification and product analysis area experimental area is provided with a medical waste transfer cabinet connected with a medical waste transfer channel.
Compared with the prior art, the invention has the following beneficial effects.
The invention discloses a nucleic acid amplification detection laboratory capable of being developed with an infection risk, which is provided with an independent material transfer channel, a sample transfer channel and a laboratory three-level protection equipment medical waste special transfer channel on the premise of ensuring that the physical spaces of a reagent storage and preparation area, a specimen preparation area, an amplification and product analysis area are completely independent, and each laboratory is provided with a buffer room, an independent one-way preparation reagent transfer channel, an independent medical waste transfer channel and a personnel one-way passage channel, so that convenient, reasonable, practical and efficient biological safety management conditions are provided for the nucleic acid amplification detection laboratory, and multiple cross contamination and biological safety potential hazards of the laboratory to laboratory areas, personnel, materials and samples in the sample, material and medical waste transfer process of the laboratory are avoided.
Drawings
FIG. 1 is a schematic diagram of a laboratory for detecting nucleic acid amplification according to an embodiment of the present invention.
FIG. 2 is a side view of a transfer cabinet of the material transfer passage.
FIG. 3 is a side view of a transfer cabinet of the sample transfer path.
FIG. 4 is a side view of the transfer cabinet with the specimen preparation area facing the sample transfer path.
Figure 5 is a side view of the medical waste transport channel facing the reagent storage and preparation area.
FIG. 6 is a side view of a medical waste delivery window of the reagent storage and preparation area.
FIG. 7 is a cross-sectional view of three channels of the specimen preparation area material delivery cabinet, the sample delivery cabinet, and the reagent storage and preparation area medical waste delivery window.
FIG. 8 is a side view of the sample delivery window of the sample preparation zone facing the amplification and product analysis zones.
FIG. 9 is a side view of the medical waste transfer channel facing the buffer compartment of the specimen preparation area.
FIG. 10 is a side view of a waste transfer cabinet in a buffer room of a specimen preparation area.
FIG. 11 is a side view of a specimen transport window in the amplification and product analysis zones.
FIG. 12 is a cross-sectional view of the medical waste delivery cabinet and sample delivery window channel between the buffer compartments of the sample preparation section.
Detailed Description
The structure, proportion, size and the like shown in the attached drawings of the specification are used for matching with the content disclosed by the specification for understanding and reading, modification of the structure, change of the proportion relation or adjustment of the size can be carried out under the condition that the effect and the achieved purpose which can be generated by the invention are not influenced, and auxiliary functions such as a changing room, a material storage area, a sample receiving area, a medical waste processing area, a wind pressure control area and the like can be arranged in related channels; the following is a more detailed description of the present invention by way of specific embodiments.
Reference numerals in the drawings of the specification include: the one-way personnel passage channel 11, the material transfer channel 21, the sample transfer channel 31, the medical waste transfer channel 41, the reagent storage and preparation area 210, the reagent storage and preparation area buffer room 211, the reagent storage and preparation area experiment area 212, the reagent storage and preparation area material transfer cabinet 311, the reagent storage and preparation area medical waste transfer window 511, the preparation reagent transfer window 411, the sample preparation area 220, the sample preparation area buffer room 221, the sample preparation area experiment area 222, the sample preparation area material transfer cabinet 321, the sample preparation area sample transfer cabinet 331, the staircase 611, the corridor 711, the sample preparation area medical waste transfer cabinet 521, the sample preparation area buffer room medical waste transfer cabinet 531, the experiment table 721, the amplification and product analysis area 230, the amplification and product analysis area buffer room 231, the amplification and product analysis area experiment area 232, the amplification and product analysis area sample transfer window 421, and the amplification and product analysis area medical waste transfer cabinet 541.
The invention provides a nucleic acid amplification detection laboratory, which consists of a one-way personnel passage channel 11, a material transfer channel 21, a sample transfer channel 31, a medical waste transfer channel 41, a reagent storage and preparation area 210, a specimen preparation area 220 and an amplification and product analysis area 230; the reagent storage and preparation area 210, the sample preparation area 220 and the amplification and product analysis area 230 are completely independent in physical space, and are respectively connected with the one-way passage 11 through the reagent storage and preparation area buffer room 211, the sample preparation area buffer room 221 and the amplification and product analysis area buffer room 231 for experimenters to enter and exit each experimental area.
A reagent storage and preparation area material transfer cabinet 311 is arranged in the reagent storage and preparation area experimental area 212 and connected with the material transfer channel 21, and is used for unidirectionally transferring materials required by the reagent storage and preparation area experimental area; a gallery 711 is arranged above the sample preparation area material transfer cabinet 321 and the sample preparation area sample transfer cabinet 331, and a stair 611 is arranged on the side surface of the sample preparation area sample transfer cabinet 331; the gallery 711 and the stairs 611 are of a closed structure, and the material transfer cabinet 321 in the specimen preparation area and the sample transfer cabinet 331 in the specimen preparation area are separated from the medical waste transfer passage 41 through the gallery 711 and the stairs 611, so that the effect of mutual independence in physical space is achieved; the reagent storage and preparation area medical waste delivery window 511 is positioned above the gallery 711 and is connected with the medical waste transfer passage 41; medical waste transport personnel carry out reagent storage and medical waste transport of the preparation area experimental area 212 through the corridor 711 by the stairs 611; the prepared reagent delivery window 411 is connected with the specimen preparation area 220 and used for unidirectional delivery of reagent storage and preparation of reagents such as amplification reaction mixed liquid and the like prepared in the preparation area experiment area.
A specimen preparation area material transfer cabinet 321 arranged in the specimen preparation area experimental area 222 is connected with the material transfer channel 21 and is used for unidirectionally transferring materials required by the specimen preparation area experimental area; the sample preparation area sample transfer cabinet 331 is connected with the sample transfer channel 31 and used for transferring the sample transfer box in a one-way mode; a sample preparation area medical waste transfer cabinet 521 is arranged to be connected with the medical waste transfer channel 41 and used for transferring medical waste generated in the sample preparation area experimental area 222 in a one-way manner; the amplification and product analysis zone sample transfer window 421 is configured to communicate with the amplification and product analysis zone experimental region 232 for unidirectional transfer of the sample preparation zone experimental region 222 for preparation of the sample.
The sample preparation area buffer room 221 is arranged between the one-way passing channel 11 of the personnel and the medical waste transfer channel 41, the sample preparation area buffer room 221 is communicated with the one-way passing channel 11 of the personnel, the sample preparation area buffer room 221 is communicated with the medical waste transfer channel 41 through a sample preparation area buffer room medical waste transfer cabinet 531, and is used for one-way transferring the medical waste of the three-stage protective equipment which is detached when the experimenter in the sample preparation area leaves the experimental area 222 of the sample preparation area; an experiment table 721 is arranged between the sample preparation area buffer medical waste transfer cabinet 531 and the sample preparation area experiment area 222, and the experiment table 721 is of a closed structure; the specimen preparation area buffer room medical waste transfer cabinet 531 is separated from the specimen preparation area experimental area 222 through the experiment table 721, so that the mutual independent effect in the physical space is realized; the amplification and product analysis zone sample delivery window 421 is located above the experimental bench 721.
The amplification and product analysis area experimental area 232 is internally provided with an amplification and product analysis area medical waste transfer cabinet 541 which is connected with the medical waste transfer channel 41 and is used for unidirectionally transferring the medical waste generated by the amplification and product analysis area experimental area 232.
Claims (1)
1. A nucleic acid amplification detection laboratory comprises a one-way personnel passage, a material transfer passage, a sample transfer passage, a medical waste transfer passage, a reagent storage and preparation area, a specimen preparation area and an amplification and product analysis area; the method is characterized in that: the three experimental areas of the reagent storage and preparation area, the sample preparation area, the amplification and product analysis area are completely independent in physical space and are respectively provided with a buffer room connected with a one-way passage channel for personnel; the reagent storage and preparation area experiment area and the specimen preparation area are respectively provided with a material transfer cabinet connected with a material transfer channel; the sample preparation area is provided with a sample transfer cabinet connected with a sample transfer channel; a closed corridor is arranged above the material transfer cabinet and the sample transfer cabinet in the sample preparation area, and a stair is arranged on the side surface of the sample transfer cabinet and is spatially separated from the material transfer cabinet, the sample transfer cabinet and the medical waste transfer channel in the sample preparation area; the reagent storage and preparation area medical waste transfer window is positioned above the gallery and is connected with the medical waste transfer channel; the reagent storage and preparation area experiment area is provided with a prepared reagent transfer window connected with the specimen preparation area; the specimen preparation area experimental area is provided with a medical waste transfer cabinet connected with a medical waste transfer channel; the sample preparation area experimental area is provided with a sample transfer window which is connected with the amplification and product analysis area experimental area; the sample preparation area buffer room is arranged between the one-way personnel passing channel and the medical waste transferring channel, the buffer room is connected with the one-way personnel passing channel, and the medical waste transferring cabinet is arranged in the buffer room and connected with the medical waste transferring channel; a closed experiment table is arranged between the medical waste transfer cabinet in the sample preparation area buffer room and the sample preparation area experiment area and is separated from the medical waste transfer cabinet and the sample preparation area experiment area space; the sample transfer window of the amplification and product analysis area is positioned above the experiment table and is connected with the experiment area of the amplification and product analysis area; and the amplification and product analysis area experimental area is provided with a medical waste transfer cabinet connected with a medical waste transfer channel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211083568.5A CN115263040B (en) | 2022-09-07 | 2022-09-07 | Nucleic acid amplification detection laboratory |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211083568.5A CN115263040B (en) | 2022-09-07 | 2022-09-07 | Nucleic acid amplification detection laboratory |
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| CN115263040A true CN115263040A (en) | 2022-11-01 |
| CN115263040B CN115263040B (en) | 2024-02-02 |
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