CN115260299A - Ginseng PgWRKY2 transcription factor and application thereof - Google Patents
Ginseng PgWRKY2 transcription factor and application thereof Download PDFInfo
- Publication number
- CN115260299A CN115260299A CN202210517926.2A CN202210517926A CN115260299A CN 115260299 A CN115260299 A CN 115260299A CN 202210517926 A CN202210517926 A CN 202210517926A CN 115260299 A CN115260299 A CN 115260299A
- Authority
- CN
- China
- Prior art keywords
- ginseng
- pgwrky2
- transcription factor
- phosphorus
- sequence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 235000008434 ginseng Nutrition 0.000 title claims abstract description 72
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 title claims abstract description 70
- 235000003140 Panax quinquefolius Nutrition 0.000 title claims abstract description 70
- 108091023040 Transcription factor Proteins 0.000 title claims abstract description 48
- 102000040945 Transcription factor Human genes 0.000 title claims abstract description 48
- 241000208340 Araliaceae Species 0.000 title claims abstract 21
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims abstract description 38
- 229910052698 phosphorus Inorganic materials 0.000 claims abstract description 38
- 239000011574 phosphorus Substances 0.000 claims abstract description 38
- 230000014509 gene expression Effects 0.000 claims abstract description 30
- 238000011144 upstream manufacturing Methods 0.000 claims description 8
- 239000002299 complementary DNA Substances 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 4
- 210000000056 organ Anatomy 0.000 claims description 3
- 238000001917 fluorescence detection Methods 0.000 claims description 2
- 239000002773 nucleotide Substances 0.000 claims description 2
- 125000003729 nucleotide group Chemical group 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 235000016709 nutrition Nutrition 0.000 abstract description 6
- 230000035764 nutrition Effects 0.000 abstract description 6
- 238000001514 detection method Methods 0.000 abstract description 5
- 230000001105 regulatory effect Effects 0.000 abstract description 5
- 108090000790 Enzymes Proteins 0.000 abstract description 3
- 102000004190 Enzymes Human genes 0.000 abstract description 3
- 230000009211 stress pathway Effects 0.000 abstract description 3
- 240000004371 Panax ginseng Species 0.000 description 51
- 241000196324 Embryophyta Species 0.000 description 8
- 238000011160 research Methods 0.000 description 6
- 238000006243 chemical reaction Methods 0.000 description 5
- 230000033228 biological regulation Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000012408 PCR amplification Methods 0.000 description 3
- 108010040443 aspartyl-aspartic acid Proteins 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 239000013612 plasmid Substances 0.000 description 3
- JLIDBLDQVAYHNE-YKALOCIXSA-N (+)-Abscisic acid Chemical compound OC(=O)/C=C(/C)\C=C\[C@@]1(O)C(C)=CC(=O)CC1(C)C JLIDBLDQVAYHNE-YKALOCIXSA-N 0.000 description 2
- XMBSYZWANAQXEV-UHFFFAOYSA-N N-alpha-L-glutamyl-L-phenylalanine Natural products OC(=O)CCC(N)C(=O)NC(C(O)=O)CC1=CC=CC=C1 XMBSYZWANAQXEV-UHFFFAOYSA-N 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- 235000002789 Panax ginseng Nutrition 0.000 description 2
- 238000011529 RT qPCR Methods 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 230000006978 adaptation Effects 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000003199 nucleic acid amplification method Methods 0.000 description 2
- 238000000746 purification Methods 0.000 description 2
- 230000004044 response Effects 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 2
- 238000012163 sequencing technique Methods 0.000 description 2
- 108010026333 seryl-proline Proteins 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- YYSWCHMLFJLLBJ-ZLUOBGJFSA-N Ala-Ala-Ser Chemical compound C[C@H](N)C(=O)N[C@@H](C)C(=O)N[C@@H](CO)C(O)=O YYSWCHMLFJLLBJ-ZLUOBGJFSA-N 0.000 description 1
- PAIHPOGPJVUFJY-WDSKDSINSA-N Ala-Glu-Gly Chemical compound C[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(O)=O PAIHPOGPJVUFJY-WDSKDSINSA-N 0.000 description 1
- 241000219194 Arabidopsis Species 0.000 description 1
- UISQLSIBJKEJSS-GUBZILKMSA-N Arg-Arg-Ser Chemical compound NC(N)=NCCC[C@H](N)C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CO)C(O)=O UISQLSIBJKEJSS-GUBZILKMSA-N 0.000 description 1
- JVMKBJNSRZWDBO-FXQIFTODSA-N Arg-Cys-Ser Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(O)=O JVMKBJNSRZWDBO-FXQIFTODSA-N 0.000 description 1
- SSZGOKWBHLOCHK-DCAQKATOSA-N Arg-Lys-Asn Chemical compound NC(=O)C[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CCCN=C(N)N SSZGOKWBHLOCHK-DCAQKATOSA-N 0.000 description 1
- RIIVUOJDDQXHRV-SRVKXCTJSA-N Arg-Lys-Gln Chemical compound [H]N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(N)=O)C(O)=O RIIVUOJDDQXHRV-SRVKXCTJSA-N 0.000 description 1
- KSBHCUSPLWRVEK-ZLUOBGJFSA-N Asn-Asn-Asp Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O KSBHCUSPLWRVEK-ZLUOBGJFSA-N 0.000 description 1
- SPIPSJXLZVTXJL-ZLUOBGJFSA-N Asn-Cys-Ser Chemical compound NC(=O)C[C@H](N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CO)C(O)=O SPIPSJXLZVTXJL-ZLUOBGJFSA-N 0.000 description 1
- COWITDLVHMZSIW-CIUDSAMLSA-N Asn-Lys-Ser Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(O)=O COWITDLVHMZSIW-CIUDSAMLSA-N 0.000 description 1
- WLVLIYYBPPONRJ-GCJQMDKQSA-N Asn-Thr-Ala Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](C)C(O)=O WLVLIYYBPPONRJ-GCJQMDKQSA-N 0.000 description 1
- RTFXPCYMDYBZNQ-SRVKXCTJSA-N Asn-Tyr-Asn Chemical compound [H]N[C@@H](CC(N)=O)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC(N)=O)C(O)=O RTFXPCYMDYBZNQ-SRVKXCTJSA-N 0.000 description 1
- XEDQMTWEYFBOIK-ACZMJKKPSA-N Asp-Ala-Glu Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](C)C(=O)N[C@@H](CCC(O)=O)C(O)=O XEDQMTWEYFBOIK-ACZMJKKPSA-N 0.000 description 1
- SVFOIXMRMLROHO-SRVKXCTJSA-N Asp-Asp-Phe Chemical compound OC(=O)C[C@H](N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@H](C(O)=O)CC1=CC=CC=C1 SVFOIXMRMLROHO-SRVKXCTJSA-N 0.000 description 1
- QCVXMEHGFUMKCO-YUMQZZPRSA-N Asp-Gly-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)CNC(=O)[C@@H](N)CC(O)=O QCVXMEHGFUMKCO-YUMQZZPRSA-N 0.000 description 1
- VWWAFGHMPWBKEP-GMOBBJLQSA-N Asp-Met-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CC(=O)O)N VWWAFGHMPWBKEP-GMOBBJLQSA-N 0.000 description 1
- UCHSVZYJKJLPHF-BZSNNMDCSA-N Asp-Phe-Phe Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O UCHSVZYJKJLPHF-BZSNNMDCSA-N 0.000 description 1
- HICVMZCGVFKTPM-BQBZGAKWSA-N Asp-Pro-Gly Chemical compound OC(=O)C[C@H](N)C(=O)N1CCC[C@H]1C(=O)NCC(O)=O HICVMZCGVFKTPM-BQBZGAKWSA-N 0.000 description 1
- RVMXMLSYBTXCAV-VEVYYDQMSA-N Asp-Pro-Thr Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(O)=O RVMXMLSYBTXCAV-VEVYYDQMSA-N 0.000 description 1
- ZQFRDAZBTSFGGW-SRVKXCTJSA-N Asp-Ser-Phe Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC1=CC=CC=C1)C(O)=O ZQFRDAZBTSFGGW-SRVKXCTJSA-N 0.000 description 1
- MGSVBZIBCCKGCY-ZLUOBGJFSA-N Asp-Ser-Ser Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(O)=O MGSVBZIBCCKGCY-ZLUOBGJFSA-N 0.000 description 1
- LTARLVHGOGBRHN-AAEUAGOBSA-N Asp-Trp-Gly Chemical compound [H]N[C@@H](CC(O)=O)C(=O)N[C@@H](CC1=CNC2=C1C=CC=C2)C(=O)NCC(O)=O LTARLVHGOGBRHN-AAEUAGOBSA-N 0.000 description 1
- OTKUAVXGMREHRX-CFMVVWHZSA-N Asp-Tyr-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC(O)=O)CC1=CC=C(O)C=C1 OTKUAVXGMREHRX-CFMVVWHZSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 108010078791 Carrier Proteins Proteins 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 108091092584 GDNA Proteins 0.000 description 1
- 108700039691 Genetic Promoter Regions Proteins 0.000 description 1
- OYTPNWYZORARHL-XHNCKOQMSA-N Gln-Ala-Pro Chemical compound C[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](CCC(=O)N)N OYTPNWYZORARHL-XHNCKOQMSA-N 0.000 description 1
- SOBBAYVQSNXYPQ-ACZMJKKPSA-N Gln-Asn-Asn Chemical compound [H]N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(N)=O)C(O)=O SOBBAYVQSNXYPQ-ACZMJKKPSA-N 0.000 description 1
- IVCOYUURLWQDJQ-LPEHRKFASA-N Gln-Gln-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CCC(=O)N)NC(=O)[C@H](CCC(=O)N)N)C(=O)O IVCOYUURLWQDJQ-LPEHRKFASA-N 0.000 description 1
- MLCPTRRNICEKIS-FXQIFTODSA-N Glu-Asn-Gln Chemical compound [H]N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(N)=O)C(O)=O MLCPTRRNICEKIS-FXQIFTODSA-N 0.000 description 1
- BUZMZDDKFCSKOT-CIUDSAMLSA-N Glu-Glu-Glu Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O BUZMZDDKFCSKOT-CIUDSAMLSA-N 0.000 description 1
- PJBVXVBTTFZPHJ-GUBZILKMSA-N Glu-Leu-Asp Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)O)NC(=O)[C@H](CCC(=O)O)N PJBVXVBTTFZPHJ-GUBZILKMSA-N 0.000 description 1
- IXKRSKPKSLXIHN-YUMQZZPRSA-N Gly-Cys-Leu Chemical compound [H]NCC(=O)N[C@@H](CS)C(=O)N[C@@H](CC(C)C)C(O)=O IXKRSKPKSLXIHN-YUMQZZPRSA-N 0.000 description 1
- JUBDONGMHASUCN-IUCAKERBSA-N Gly-Glu-His Chemical compound NCC(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](Cc1cnc[nH]1)C(O)=O JUBDONGMHASUCN-IUCAKERBSA-N 0.000 description 1
- WNGHUXFWEWTKAO-YUMQZZPRSA-N Gly-Ser-Leu Chemical compound CC(C)C[C@@H](C(O)=O)NC(=O)[C@H](CO)NC(=O)CN WNGHUXFWEWTKAO-YUMQZZPRSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- BIAKMWKJMQLZOJ-ZKWXMUAHSA-N His-Ala-Ala Chemical compound C[C@H](NC(=O)[C@H](C)NC(=O)[C@@H](N)Cc1cnc[nH]1)C(O)=O BIAKMWKJMQLZOJ-ZKWXMUAHSA-N 0.000 description 1
- GJMHMDKCJPQJOI-IHRRRGAJSA-N His-Lys-Arg Chemical compound NC(N)=NCCC[C@@H](C(O)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CC1=CN=CN1 GJMHMDKCJPQJOI-IHRRRGAJSA-N 0.000 description 1
- IGBBXBFSLKRHJB-BZSNNMDCSA-N His-Lys-Phe Chemical compound C([C@H](N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CN=CN1 IGBBXBFSLKRHJB-BZSNNMDCSA-N 0.000 description 1
- OWYIDJCNRWRSJY-QTKMDUPCSA-N His-Pro-Thr Chemical compound [H]N[C@@H](CC1=CNC=N1)C(=O)N1CCC[C@H]1C(=O)N[C@@H]([C@@H](C)O)C(O)=O OWYIDJCNRWRSJY-QTKMDUPCSA-N 0.000 description 1
- XVZJRZQIHJMUBG-TUBUOCAGSA-N His-Thr-Ile Chemical compound CC[C@H](C)[C@@H](C(=O)O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@H](CC1=CN=CN1)N XVZJRZQIHJMUBG-TUBUOCAGSA-N 0.000 description 1
- 240000005979 Hordeum vulgare Species 0.000 description 1
- 235000007340 Hordeum vulgare Nutrition 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- LKACSKJPTFSBHR-MNXVOIDGSA-N Ile-Gln-Lys Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCC(=O)N)C(=O)N[C@@H](CCCCN)C(=O)O)N LKACSKJPTFSBHR-MNXVOIDGSA-N 0.000 description 1
- WUKLZPHVWAMZQV-UKJIMTQDSA-N Ile-Glu-Val Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](C(C)C)C(=O)O)N WUKLZPHVWAMZQV-UKJIMTQDSA-N 0.000 description 1
- PDTMWFVVNZYWTR-NHCYSSNCSA-N Ile-Gly-Lys Chemical compound CC[C@H](C)[C@H](N)C(=O)NCC(=O)N[C@@H](CCCCN)C(O)=O PDTMWFVVNZYWTR-NHCYSSNCSA-N 0.000 description 1
- AXNGDPAKKCEKGY-QPHKQPEJSA-N Ile-Ile-Thr Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)N[C@@H]([C@@H](C)O)C(=O)O)N AXNGDPAKKCEKGY-QPHKQPEJSA-N 0.000 description 1
- XDUVMJCBYUKNFJ-MXAVVETBSA-N Ile-Lys-His Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC1=CN=CN1)C(=O)O)N XDUVMJCBYUKNFJ-MXAVVETBSA-N 0.000 description 1
- PXKACEXYLPBMAD-JBDRJPRFSA-N Ile-Ser-Ser Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)O)N PXKACEXYLPBMAD-JBDRJPRFSA-N 0.000 description 1
- ANTFEOSJMAUGIB-KNZXXDILSA-N Ile-Thr-Pro Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H]([C@@H](C)O)C(=O)N1CCC[C@@H]1C(=O)O)N ANTFEOSJMAUGIB-KNZXXDILSA-N 0.000 description 1
- YJRSIJZUIUANHO-NAKRPEOUSA-N Ile-Val-Ala Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](C)C(=O)O)N YJRSIJZUIUANHO-NAKRPEOUSA-N 0.000 description 1
- WNGVUZWBXZKQES-YUMQZZPRSA-N Leu-Ala-Gly Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](C)C(=O)NCC(O)=O WNGVUZWBXZKQES-YUMQZZPRSA-N 0.000 description 1
- VCSBGUACOYUIGD-CIUDSAMLSA-N Leu-Asn-Asp Chemical compound [H]N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(O)=O)C(O)=O VCSBGUACOYUIGD-CIUDSAMLSA-N 0.000 description 1
- VQPPIMUZCZCOIL-GUBZILKMSA-N Leu-Gln-Ala Chemical compound CC(C)C[C@H](N)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](C)C(O)=O VQPPIMUZCZCOIL-GUBZILKMSA-N 0.000 description 1
- RTIRBWJPYJYTLO-MELADBBJSA-N Leu-Lys-Pro Chemical compound CC(C)C[C@@H](C(=O)N[C@@H](CCCCN)C(=O)N1CCC[C@@H]1C(=O)O)N RTIRBWJPYJYTLO-MELADBBJSA-N 0.000 description 1
- CNWDWAMPKVYJJB-NUTKFTJISA-N Leu-Trp-Ala Chemical compound C1=CC=C2C(C[C@H](NC(=O)[C@@H](N)CC(C)C)C(=O)N[C@@H](C)C(O)=O)=CNC2=C1 CNWDWAMPKVYJJB-NUTKFTJISA-N 0.000 description 1
- 241000209082 Lolium Species 0.000 description 1
- FHIAJWBDZVHLAH-YUMQZZPRSA-N Lys-Gly-Ser Chemical compound NCCCC[C@H](N)C(=O)NCC(=O)N[C@@H](CO)C(O)=O FHIAJWBDZVHLAH-YUMQZZPRSA-N 0.000 description 1
- QBHGXFQJFPWJIH-XUXIUFHCSA-N Lys-Pro-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CCCCN QBHGXFQJFPWJIH-XUXIUFHCSA-N 0.000 description 1
- WZVSHTFTCYOFPL-GARJFASQSA-N Lys-Ser-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CO)NC(=O)[C@H](CCCCN)N)C(=O)O WZVSHTFTCYOFPL-GARJFASQSA-N 0.000 description 1
- WINFHLHJTRGLCV-BZSNNMDCSA-N Lys-Tyr-Lys Chemical compound NCCCC[C@H](N)C(=O)N[C@H](C(=O)N[C@@H](CCCCN)C(O)=O)CC1=CC=C(O)C=C1 WINFHLHJTRGLCV-BZSNNMDCSA-N 0.000 description 1
- GPAHWYRSHCKICP-GUBZILKMSA-N Met-Glu-Glu Chemical compound CSCC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O GPAHWYRSHCKICP-GUBZILKMSA-N 0.000 description 1
- YCUSPBPZVJDMII-YUMQZZPRSA-N Met-Gly-Glu Chemical compound CSCC[C@H](N)C(=O)NCC(=O)N[C@H](C(O)=O)CCC(O)=O YCUSPBPZVJDMII-YUMQZZPRSA-N 0.000 description 1
- RSOMVHWMIAZNLE-HJWJTTGWSA-N Met-Phe-Ile Chemical compound [H]N[C@@H](CCSC)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O RSOMVHWMIAZNLE-HJWJTTGWSA-N 0.000 description 1
- WUGMRIBZSVSJNP-UHFFFAOYSA-N N-L-alanyl-L-tryptophan Natural products C1=CC=C2C(CC(NC(=O)C(N)C)C(O)=O)=CNC2=C1 WUGMRIBZSVSJNP-UHFFFAOYSA-N 0.000 description 1
- CDNPIRSCAFMMBE-SRVKXCTJSA-N Phe-Asn-Ser Chemical compound [H]N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(O)=O CDNPIRSCAFMMBE-SRVKXCTJSA-N 0.000 description 1
- MGBRZXXGQBAULP-DRZSPHRISA-N Phe-Glu-Ala Chemical compound OC(=O)[C@H](C)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](N)CC1=CC=CC=C1 MGBRZXXGQBAULP-DRZSPHRISA-N 0.000 description 1
- QSWKNJAPHQDAAS-MELADBBJSA-N Phe-Ser-Pro Chemical compound C1C[C@@H](N(C1)C(=O)[C@H](CO)NC(=O)[C@H](CC2=CC=CC=C2)N)C(=O)O QSWKNJAPHQDAAS-MELADBBJSA-N 0.000 description 1
- BPIMVBKDLSBKIJ-FCLVOEFKSA-N Phe-Thr-Phe Chemical compound C([C@H](N)C(=O)N[C@@H]([C@H](O)C)C(=O)N[C@@H](CC=1C=CC=CC=1)C(O)=O)C1=CC=CC=C1 BPIMVBKDLSBKIJ-FCLVOEFKSA-N 0.000 description 1
- IEIFEYBAYFSRBQ-IHRRRGAJSA-N Phe-Val-Ser Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](CC1=CC=CC=C1)N IEIFEYBAYFSRBQ-IHRRRGAJSA-N 0.000 description 1
- 102000045595 Phosphoprotein Phosphatases Human genes 0.000 description 1
- 108700019535 Phosphoprotein Phosphatases Proteins 0.000 description 1
- FYQSMXKJYTZYRP-DCAQKATOSA-N Pro-Ala-Lys Chemical compound NCCCC[C@@H](C(O)=O)NC(=O)[C@H](C)NC(=O)[C@@H]1CCCN1 FYQSMXKJYTZYRP-DCAQKATOSA-N 0.000 description 1
- AMBLXEMWFARNNQ-DCAQKATOSA-N Pro-Asn-Leu Chemical compound CC(C)C[C@@H](C(=O)O)NC(=O)[C@H](CC(=O)N)NC(=O)[C@@H]1CCCN1 AMBLXEMWFARNNQ-DCAQKATOSA-N 0.000 description 1
- FRKBNXCFJBPJOL-GUBZILKMSA-N Pro-Glu-Glu Chemical compound [H]N1CCC[C@H]1C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O FRKBNXCFJBPJOL-GUBZILKMSA-N 0.000 description 1
- 238000002123 RNA extraction Methods 0.000 description 1
- 108091006597 SLC15A4 Proteins 0.000 description 1
- UBRXAVQWXOWRSJ-ZLUOBGJFSA-N Ser-Asn-Asp Chemical compound C([C@@H](C(=O)N[C@@H](CC(=O)O)C(=O)O)NC(=O)[C@H](CO)N)C(=O)N UBRXAVQWXOWRSJ-ZLUOBGJFSA-N 0.000 description 1
- QPFJSHSJFIYDJZ-GHCJXIJMSA-N Ser-Asp-Ile Chemical compound CC[C@H](C)[C@@H](C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](N)CO QPFJSHSJFIYDJZ-GHCJXIJMSA-N 0.000 description 1
- ZHYMUFQVKGJNRM-ZLUOBGJFSA-N Ser-Cys-Asn Chemical compound OC[C@H](N)C(=O)N[C@@H](CS)C(=O)N[C@H](C(O)=O)CC(N)=O ZHYMUFQVKGJNRM-ZLUOBGJFSA-N 0.000 description 1
- UOLGINIHBRIECN-FXQIFTODSA-N Ser-Glu-Glu Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(O)=O UOLGINIHBRIECN-FXQIFTODSA-N 0.000 description 1
- CAOYHZOWXFFAIR-CIUDSAMLSA-N Ser-His-Ser Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC1=CNC=N1)C(=O)N[C@@H](CO)C(O)=O CAOYHZOWXFFAIR-CIUDSAMLSA-N 0.000 description 1
- HEUVHBXOVZONPU-BJDJZHNGSA-N Ser-Leu-Ile Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H]([C@@H](C)CC)C(O)=O HEUVHBXOVZONPU-BJDJZHNGSA-N 0.000 description 1
- UBRMZSHOOIVJPW-SRVKXCTJSA-N Ser-Leu-Lys Chemical compound OC[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(O)=O UBRMZSHOOIVJPW-SRVKXCTJSA-N 0.000 description 1
- FLONGDPORFIVQW-XGEHTFHBSA-N Ser-Pro-Thr Chemical compound C[C@@H](O)[C@@H](C(O)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CO FLONGDPORFIVQW-XGEHTFHBSA-N 0.000 description 1
- PPCZVWHJWJFTFN-ZLUOBGJFSA-N Ser-Ser-Asp Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(O)=O)C(O)=O PPCZVWHJWJFTFN-ZLUOBGJFSA-N 0.000 description 1
- JURQXQBJKUHGJS-UHFFFAOYSA-N Ser-Ser-Ser-Ser Chemical compound OCC(N)C(=O)NC(CO)C(=O)NC(CO)C(=O)NC(CO)C(O)=O JURQXQBJKUHGJS-UHFFFAOYSA-N 0.000 description 1
- WUXCHQZLUHBSDJ-LKXGYXEUSA-N Ser-Thr-Asp Chemical compound OC[C@H](N)C(=O)N[C@@H]([C@H](O)C)C(=O)N[C@@H](CC(O)=O)C(O)=O WUXCHQZLUHBSDJ-LKXGYXEUSA-N 0.000 description 1
- SNXUIBACCONSOH-BWBBJGPYSA-N Ser-Thr-Ser Chemical compound OC[C@H](N)C(=O)N[C@@H]([C@H](O)C)C(=O)N[C@@H](CO)C(O)=O SNXUIBACCONSOH-BWBBJGPYSA-N 0.000 description 1
- OSFZCEQJLWCIBG-BZSNNMDCSA-N Ser-Tyr-Tyr Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O OSFZCEQJLWCIBG-BZSNNMDCSA-N 0.000 description 1
- PCMZJFMUYWIERL-ZKWXMUAHSA-N Ser-Val-Asn Chemical compound [H]N[C@@H](CO)C(=O)N[C@@H](C(C)C)C(=O)N[C@@H](CC(N)=O)C(O)=O PCMZJFMUYWIERL-ZKWXMUAHSA-N 0.000 description 1
- IRKWVRSEQFTGGV-VEVYYDQMSA-N Thr-Asn-Arg Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O IRKWVRSEQFTGGV-VEVYYDQMSA-N 0.000 description 1
- MEBDIIKMUUNBSB-RPTUDFQQSA-N Thr-Phe-Tyr Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CC1=CC=CC=C1)C(=O)N[C@@H](CC1=CC=C(O)C=C1)C(O)=O MEBDIIKMUUNBSB-RPTUDFQQSA-N 0.000 description 1
- GFRIEEKFXOVPIR-RHYQMDGZSA-N Thr-Pro-Lys Chemical compound C[C@@H](O)[C@H](N)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(O)=O GFRIEEKFXOVPIR-RHYQMDGZSA-N 0.000 description 1
- RVMNUBQWPVOUKH-HEIBUPTGSA-N Thr-Ser-Thr Chemical compound [H]N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N[C@@H]([C@@H](C)O)C(O)=O RVMNUBQWPVOUKH-HEIBUPTGSA-N 0.000 description 1
- CYCGARJWIQWPQM-YJRXYDGGSA-N Thr-Tyr-Ser Chemical compound C[C@@H](O)[C@H]([NH3+])C(=O)N[C@H](C(=O)N[C@@H](CO)C([O-])=O)CC1=CC=C(O)C=C1 CYCGARJWIQWPQM-YJRXYDGGSA-N 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 241000209140 Triticum Species 0.000 description 1
- MVHHTXAUJCIOMZ-WDSOQIARSA-N Trp-Arg-Lys Chemical compound C1=CC=C2C(=C1)C(=CN2)C[C@@H](C(=O)N[C@@H](CCCN=C(N)N)C(=O)N[C@@H](CCCCN)C(=O)O)N MVHHTXAUJCIOMZ-WDSOQIARSA-N 0.000 description 1
- JWGXUKHIKXZWNG-RYUDHWBXSA-N Tyr-Gly-Gln Chemical compound C1=CC(=CC=C1C[C@@H](C(=O)NCC(=O)N[C@@H](CCC(=O)N)C(=O)O)N)O JWGXUKHIKXZWNG-RYUDHWBXSA-N 0.000 description 1
- LMKKMCGTDANZTR-BZSNNMDCSA-N Tyr-Phe-Asp Chemical compound C([C@H](N)C(=O)N[C@@H](CC=1C=CC=CC=1)C(=O)N[C@@H](CC(O)=O)C(O)=O)C1=CC=C(O)C=C1 LMKKMCGTDANZTR-BZSNNMDCSA-N 0.000 description 1
- AUZADXNWQMBZOO-JYJNAYRXSA-N Tyr-Pro-Arg Chemical compound C([C@H](N)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCCN=C(N)N)C(O)=O)C1=CC=C(O)C=C1 AUZADXNWQMBZOO-JYJNAYRXSA-N 0.000 description 1
- COYSIHFOCOMGCF-WPRPVWTQSA-N Val-Arg-Gly Chemical compound CC(C)[C@H](N)C(=O)N[C@H](C(=O)NCC(O)=O)CCCN=C(N)N COYSIHFOCOMGCF-WPRPVWTQSA-N 0.000 description 1
- COYSIHFOCOMGCF-UHFFFAOYSA-N Val-Arg-Gly Natural products CC(C)C(N)C(=O)NC(C(=O)NCC(O)=O)CCCN=C(N)N COYSIHFOCOMGCF-UHFFFAOYSA-N 0.000 description 1
- VLDMQVZZWDOKQF-AUTRQRHGSA-N Val-Glu-Gln Chemical compound CC(C)[C@@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)N)C(=O)O)N VLDMQVZZWDOKQF-AUTRQRHGSA-N 0.000 description 1
- ROLGIBMFNMZANA-GVXVVHGQSA-N Val-Glu-Leu Chemical compound CC(C)C[C@@H](C(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](C(C)C)N ROLGIBMFNMZANA-GVXVVHGQSA-N 0.000 description 1
- JZWZACGUZVCQPS-RNJOBUHISA-N Val-Ile-Pro Chemical compound CC[C@H](C)[C@@H](C(=O)N1CCC[C@@H]1C(=O)O)NC(=O)[C@H](C(C)C)N JZWZACGUZVCQPS-RNJOBUHISA-N 0.000 description 1
- OVBMCNDKCWAXMZ-NAKRPEOUSA-N Val-Ile-Ser Chemical compound CC[C@H](C)[C@@H](C(=O)N[C@@H](CO)C(=O)O)NC(=O)[C@H](C(C)C)N OVBMCNDKCWAXMZ-NAKRPEOUSA-N 0.000 description 1
- AEMPCGRFEZTWIF-IHRRRGAJSA-N Val-Leu-Lys Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(O)=O AEMPCGRFEZTWIF-IHRRRGAJSA-N 0.000 description 1
- CEKSLIVSNNGOKH-KZVJFYERSA-N Val-Thr-Ala Chemical compound C[C@H]([C@@H](C(=O)N[C@@H](C)C(=O)O)NC(=O)[C@H](C(C)C)N)O CEKSLIVSNNGOKH-KZVJFYERSA-N 0.000 description 1
- VVIZITNVZUAEMI-DLOVCJGASA-N Val-Val-Gln Chemical compound CC(C)[C@H](N)C(=O)N[C@@H](C(C)C)C(=O)N[C@H](C(O)=O)CCC(N)=O VVIZITNVZUAEMI-DLOVCJGASA-N 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 108010070944 alanylhistidine Proteins 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 229940093740 amino acid and derivative Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 108010069926 arginyl-glycyl-serine Proteins 0.000 description 1
- 108010092854 aspartyllysine Proteins 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- FCRACOPGPMPSHN-UHFFFAOYSA-N desoxyabscisic acid Natural products OC(=O)C=C(C)C=CC1C(C)=CC(=O)CC1(C)C FCRACOPGPMPSHN-UHFFFAOYSA-N 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012154 double-distilled water Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 229930003935 flavonoid Natural products 0.000 description 1
- 150000002215 flavonoids Chemical class 0.000 description 1
- 235000017173 flavonoids Nutrition 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 108010049041 glutamylalanine Proteins 0.000 description 1
- 238000012165 high-throughput sequencing Methods 0.000 description 1
- 108010018006 histidylserine Proteins 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 108010090333 leucyl-lysyl-proline Proteins 0.000 description 1
- 108010003700 lysyl aspartic acid Proteins 0.000 description 1
- 108010025153 lysyl-alanyl-alanine Proteins 0.000 description 1
- 108010045397 lysyl-tyrosyl-lysine Proteins 0.000 description 1
- 230000003050 macronutrient Effects 0.000 description 1
- 235000021073 macronutrients Nutrition 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000037353 metabolic pathway Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000009456 molecular mechanism Effects 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 1
- 238000007747 plating Methods 0.000 description 1
- 238000012257 pre-denaturation Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000010839 reverse transcription Methods 0.000 description 1
- 229960004889 salicylic acid Drugs 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 108010071207 serylmethionine Proteins 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 108010033670 threonyl-aspartyl-tyrosine Proteins 0.000 description 1
- 230000002103 transcriptional effect Effects 0.000 description 1
- 230000001131 transforming effect Effects 0.000 description 1
- 108010071635 tyrosyl-prolyl-arginine Proteins 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
- C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/13—Plant traits
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/158—Expression markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Mycology (AREA)
- Gastroenterology & Hepatology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention relates to the field of biotechnology, in particular to a ginseng PgWRKY2 transcription factor and application thereof; the invention discloses a regulating effect of ginseng PgWRKY2 transcription factor on phosphorus nutrition, realizes detection of phosphorus nutrition stress in ginseng adversity stress by revealing expression specificity of key enzyme in ginseng phosphorus stress pathway, thereby providing effective regulating measures and having important application value.
Description
Technical Field
The invention relates to the technical field of biology, in particular to a ginseng PgWRKY2 transcription factor and application thereof.
Background
Phosphorus (P) is a necessary macronutrient element for plants, participates in various metabolic processes in the plants and plays an important role in the growth and development of the plants. Plant adaptation to the phosphorus environment includes morphological physiological basis and gene expression regulation. Such as regulation of Phosphorus Transporters (PTs), secretion of amino acids and derivatives, flavonoids, phosphatases, and alterations of root structure. The metabolic pathway of phosphorus is regulated by a variety of genes, which are expressed differently under different phosphorus conditions. As in rice, osPHR2, a major transcriptional regulator of low phosphorus response, regulates adaptation to the phosphorus environment by activating the binding of the phosphorus starvation-induced gene PHT1 to the P1BS (PHR 1 binding sequence GNATATATNC) motif in the promoter region of the gene.
In addition, the molecular mechanism of plant response to phosphorus stress has also been studied in crops such as corn, soybean, ryegrass, barley and wheat. At present, in the aspect of researching plant phosphorus stress, the research is mainly concentrated in mode plants such as arabidopsis, rice, corn and the like and field crops, the research in medicinal plants is very little, the research in ginseng is not yet reported, meanwhile, the research in WRKY type transcription factors is also less, although the research reports that a plurality of WRKY type transcription factors in ginseng are induced and expressed by hormones such as salicylic acid, abscisic acid, na Cl and the like and salts, the research does not find that the WRKY type transcription factors specifically expressed by ginseng under the induction of phosphorus stress, and a marker and a technical method for detecting the phosphorus stress of ginseng from the gene expression level are not available.
Disclosure of Invention
Aiming at the defects in the prior art, the invention discloses the regulation and control effect of the ginseng PgWRKY2 transcription factor on phosphorus nutrition, and realizes the detection of the phosphorus nutrition stress in the ginseng adversity stress by disclosing the expression specificity of key enzymes in the ginseng phosphorus stress pathway, thereby providing effective regulation and control measures and having important application value.
In order to achieve the purpose, the invention provides the following technical scheme:
the nucleotide sequence of the ginseng PgWRKY2 transcription factor is shown in SEQ ID No. 1;
the coding protein sequence of the ginseng PgWRKY2 transcription factor is shown in SEQ ID No. 2.
Further, the cDNA sequence primer pair for expanding the ginseng PgWRKY2 transcription factor comprises: the sequence of the upstream primer is shown as SEQID No.3, and the sequence of the downstream primer is shown as SEQID No. 4.
Further, the primer pair for fluorescence detection of the ginseng PgWRKY2 transcription factor is as follows: the sequence of the upstream primer is shown as SEQID No.5, and the sequence of the downstream primer is shown as SEQID No. 6.
The invention also provides application of the ginseng PgWRKY2 transcription factor in ginseng organ tissues, wherein the ginseng PgWRKY2 transcription factor is expressed in the main root, the lateral root, the stem and the leaf of the ginseng, the expression level of the ginseng PgWRKY2 transcription factor in the lateral root and the main root is highest, and the expression level of the ginseng PgWRKY2 transcription factor in the leaf and the stem is lowest.
In addition, the invention also provides application of the ginseng PgWRKY2 transcription factor in improving stress resistance of ginseng under stress of different exogenous phosphorus concentrations, wherein the ginseng PgWRKY2 transcription factor has the highest expression level at the concentration of 2.0mM, has the lower expression level at the concentration of 4.0mM and has the expression level relatively lower at the concentrations of 0mM, 0.5mM and 1.0mM, and the expression level increases from the concentration of 0mM to the concentration of 2.0mM along with the increase of the phosphorus concentration and shows a positive correlation trend.
Advantageous effects
Compared with the known public technology, the technical scheme provided by the invention has the following beneficial effects:
the invention discloses a regulating effect of a ginseng PgWRKY2 transcription factor on phosphorus nutrition, in particular to a method for detecting phosphorus nutrition stress in ginseng adversity stress by responding to phosphorus stress expression of the ginseng PgWRKY2 transcription factor and disclosing expression specificity of key enzymes in a ginseng phosphorus stress pathway, thereby providing an effective regulating measure and having important application value.
Drawings
FIG. 1 is a schematic diagram of the purification and recovery of a target band according to the present invention;
FIG. 2 is a schematic diagram showing the expression of the PgWRKY2 transcription factor of ginseng in the main root, lateral root, stem and leaf of ginseng;
FIG. 3 is a schematic diagram of the expression of the ginseng PgWRKY2 transcription factor under different phosphorus concentration stresses.
Detailed Description
The invention identifies a WRKY type transcription factor PgWRKY2 responding to phosphorus stress from ginseng through high-throughput sequencing, designs a PgWRKY2 sequence PCR amplification Primer and a real-time fluorescent quantitative PCR (qRT-PCR) detection Primer by using DNAMAN and Primer Premier 6 software based on a transcriptome database, and provides a new strategy for transforming ginseng callus by using genetic engineering in the future by researching the application of the PgWRKY2 transcription factor in responding to the phosphorus stress of the ginseng, thereby improving the stress resistance of the ginseng.
The present invention will be further described with reference to the following examples.
Example 1: RNA extraction of ginseng rhizome tissue
Adopting biennial ginseng seedlings of ginseng planting bases in Fusong county, jilin province, in an intelligent artificial climate chamber (temperature 20 ℃, illumination 2000 lx) in a laboratory, carrying out water planting by using Hoagland nutrient solutions with different phosphorus concentrations, and setting 5 phosphorus concentration levels: p0 (0 mM), P1 (0.5 mM), P2 (1.0 mM), P3 (2.0 mM) and P4 (4.0 mM), and the ginseng seedlings are cultured in water until sampling is carried out once in the eighth week; RNA of ginseng rhizome tissues is extracted by using an RNAscope Total RNA Kit.
Example 2: PCR amplification
After reverse transcription into cDNA by FastKing gDNA dispensing RT SuperMix kit, 2 × Trans Taq was usedFidelity (HiFi) PCR Supermix II to perform PgWRKY2 sequence amplification (reaction system: 12.5. Mu.L 2 × Trans TaqFidelity (HiFi) PCR Supermix II, 1. Mu.L of each of the upstream and downstream primers (10. Mu. Mol/L), 1. Mu.L of template, 9.5. Mu.L of ddH2O; the reaction procedure is as follows: pre-denaturation at 95 ℃ for 4min; 35 cycles of 95 deg.C, 30s,51 deg.C, 30s,72 deg.C, 1min, 30 s; 72 deg.C, 7min. ).
Wherein, during amplification:
the upstream primer is as follows: pgWRKY2-F:5 'ATGGGAGAATTTGTTAGTATGGAG-3' (SEQ ID No. 3)
The downstream primer is: pgWRKY2-R:5 'TTACCTAAAATGTTCCGTAGAGCA-3' (SEQ ID No. 4);
after the PCR amplification reaction was completed, the TIANgel Midi purification Kit was used for the testPurifying and recovering target strip with kit (shown in FIG. 1); using the destination stripConnecting a T5 Zero Cloning Kit to a T5 vector, plating transformed escherichia coli, picking monoclonal shake bacteria, extracting plasmids by using a TIANPrep Mini Plasmid Kit, and sending the plasmids to Shanghai bio-chemical company for sequencing; the sequencing results were analyzed using DNAMAN.
Example 3: pgWRKY2 transcription factor
RNA of main root, lateral root, stem and leaf tissue of ginseng is extracted by using an RNAscope Total RNA Kit, and is reversely transcribed into cDNA serving as a template to carry out qRT-PCR detection analysis (the reaction system is FastStart Universal 2X SYBR Green 12.5 mu L, each of an upstream primer and a downstream primer is 0.5 mu L (10 mu mol/L), a cDNA template is 1 mu L, and 10.5 mu L dd H is added2O, simultaneously with 1. Mu.L of dd H2O as a negative control instead of template; the reaction procedure is as follows: 94 ℃ for 2min; 40 cycles of 94 ℃ at 15s,55 ℃ at 20s,72 ℃ at 30 s; 72 deg.C, 7min. ).
The real-time fluorescent quantitative detection primer pair for the PgWRKY2 transcription factor of the ginseng is as follows:
an upstream primer: pgWRKY2q-F:5 'TCATTCGGTTCACTGGATTACA-3' (SEQ ID No. 5)
A downstream primer: pgWRKY2q-R:5 'GCTCATCTAACTCATCCAAAG-doped 3' (SEQ ID No. 6);
each sample was set up for 3 technical and biological replicates, using 2-ΔΔCtRelative expression levels were calculated.
Experimental results show that the full length of the PgWRKY2 transcription factor is 975bp (SEQID No. 1), and 324 amino acids (SEQID No. 2) are coded; the transcription factor is obviously expressed in roots of the ginseng, and the expression quantity of PgWRKY2 and the phosphorus concentration have a positive correlation trend within a certain exogenous phosphorus concentration range, namely the higher the exogenous phosphorus concentration is, the higher the expression quantity of the PgWRKY2 transcription factor is, and accordingly, the condition that the ginseng is stressed by phosphorus can be preliminarily detected.
Example 4: detected expression pattern of PgWRKY2 transcription factor in ginseng organ tissue
As shown in figure 2, the ginseng PgWRKY2 transcription factor is expressed in the main root, lateral root, stem and leaf of ginseng, but the expression is obviously different, the expression level is highest in the lateral root and main root, and the expression level is lowest in the stem and leaf.
Example 5: expression mode of ginseng PgWRKY2 transcription factor under stress of different phosphorus concentrations
As shown in FIG. 3, under exogenous phosphorus treatment, the PgWRKY2 transcription factor was expressed in the highest amount at the P3 (2.0 mM) concentration, the next time at the P4 (4.0 mM) concentration, the expression levels were relatively low at the P0 (0 mM), P1 (0.5 mM) and P2 (1.0 mM) concentrations, and the expression increased from the P0 (0 mM) concentration to the P3 (2.0 mM) concentration with increasing phosphorus concentration, with a positive correlation trend.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; such modifications and substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Sequence listing
<110> university of Chengdu
<120> ginseng PgWRKY2 transcription factor and application thereof
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 975
<212> DNA
<213> Ginseng radix (Panax ginseng C.A. Meyer)
<400> 1
atgggagaat ttgttagtat ggaggaagat tggggtctgc aagctattgt cagaggatcc 60
accgattatt ttgatgatcc gactatttct tcattcggtt cactggatta cattcagaat 120
aataataacg atgatgattt tcatttcact tttcctaatc tttttgaagc caattataac 180
agtaatgata aagttcttgt ggatgagtta gatgagcttt acaagccctt ttatcccatg 240
tttaattctt tttccccaca agctcccatt attacctcct cctcctccga ttctttccct 300
gaagaagtcc tgaagttaaa gccagaaaat caagagatcg aagttatcca gaagcacaca 360
attcctgcta aagctgctag tcctaataaa agtaatactg cccatgcagc taaatataaa 420
aggaagaacc aacacaaaag ggtggtggtt caagtaactg ctgatggtct ttcttctgat 480
ttgtgggctt ggcgtaaata tggtcagaaa cccattaaag gatcacctta tccaaggagc 540
tattataggt gtagtagctt aaaaggatgt ttggcaagga agcaagttga acaaagctgt 600
aatgatcccg gaatgtttat cataacctat tccggggaac atagccacag ccatccaact 660
cgacggagtt ctcttgccgg tacaaaccgg cacaagttca tcaccccgaa aagcccctca 720
tcggtcaata ccagtactat tgtggcaaca cccaaagact ccagttgctc tccaacatca 780
gatatcagtg aagaagtagt aattccccag cagcccatta aacatgaaga agaggctgaa 840
ggaatcggca aggatgctga atttgttata tcagatatga tcttgaacga cgatttcttt 900
gtggaattag aggagttgga ttccctaatt tcaacttcaa ccttttacaa ttgctctacg 960
gaacatttta ggtaa 975
<210> 2
<211> 324
<212> PRT
<213> Ginseng radix (Panax ginseng C.A. Meyer)
<400> 2
Met Gly Glu Phe Val Ser Met Glu Glu Asp Trp Gly Leu Gln Ala Ile
1 5 10 15
Val Arg Gly Ser Thr Asp Tyr Phe Asp Asp Pro Thr Ile Ser Ser Phe
20 25 30
Gly Ser Leu Asp Tyr Ile Gln Asn Asn Asn Asn Asp Asp Asp Phe His
35 40 45
Phe Thr Phe Pro Asn Leu Phe Glu Ala Asn Tyr Asn Ser Asn Asp Lys
50 55 60
Val Leu Val Asp Glu Leu Asp Glu Leu Tyr Lys Pro Phe Tyr Pro Met
65 70 75 80
Phe Asn Ser Phe Ser Pro Gln Ala Pro Ile Ile Thr Ser Ser Ser Ser
85 90 95
Asp Ser Phe Pro Glu Glu Val Leu Lys Leu Lys Pro Glu Asn Gln Glu
100 105 110
Ile Glu Val Ile Gln Lys His Thr Ile Pro Ala Lys Ala Ala Ser Pro
115 120 125
Asn Lys Ser Asn Thr Ala His Ala Ala Lys Tyr Lys Arg Lys Asn Gln
130 135 140
His Lys Arg Val Val Val Gln Val Thr Ala Asp Gly Leu Ser Ser Asp
145 150 155 160
Leu Trp Ala Trp Arg Lys Tyr Gly Gln Lys Pro Ile Lys Gly Ser Pro
165 170 175
Tyr Pro Arg Ser Tyr Tyr Arg Cys Ser Ser Leu Lys Gly Cys Leu Ala
180 185 190
Arg Lys Gln Val Glu Gln Ser Cys Asn Asp Pro Gly Met Phe Ile Ile
195 200 205
Thr Tyr Ser Gly Glu His Ser His Ser His Pro Thr Arg Arg Ser Ser
210 215 220
Leu Ala Gly Thr Asn Arg His Lys Phe Ile Thr Pro Lys Ser Pro Ser
225 230 235 240
Ser Val Asn Thr Ser Thr Ile Val Ala Thr Pro Lys Asp Ser Ser Cys
245 250 255
Ser Pro Thr Ser Asp Ile Ser Glu Glu Val Val Ile Pro Gln Gln Pro
260 265 270
Ile Lys His Glu Glu Glu Ala Glu Gly Ile Gly Lys Asp Ala Glu Phe
275 280 285
Val Ile Ser Asp Met Ile Leu Asn Asp Asp Phe Phe Val Glu Leu Glu
290 295 300
Glu Leu Asp Ser Leu Ile Ser Thr Ser Thr Phe Tyr Asn Cys Ser Thr
305 310 315 320
Glu His Phe Arg
Claims (5)
1. The ginseng PgWRKY2 transcription factor is characterized in that the nucleotide sequence of the ginseng PgWRKY2 transcription factor is shown as SEQ ID No. 1;
the coding protein sequence of the ginseng PgWRKY2 transcription factor is shown in SEQ ID No. 2.
2. The ginseng PgWRKY2 transcription factor as claimed in claim 1, wherein the cDNA sequence primer pair for expanding the ginseng PgWRKY2 transcription factor is: the sequence of the upstream primer is shown as SEQ ID No.3, and the sequence of the downstream primer is shown as SEQ ID No. 4.
3. The ginseng PgWRKY2 transcription factor as claimed in claim 1, wherein the primer pair for fluorescence detection of the ginseng PgWRKY2 transcription factor is as follows: the sequence of the upstream primer is shown as SEQ ID No.5, and the sequence of the downstream primer is shown as SEQ ID No. 6.
4. The application of the ginseng PgWRKY2 transcription factor in the organ tissues of the ginseng is characterized in that the ginseng PgWRKY2 transcription factor is expressed in the main root, the lateral root, the stem and the leaf of the ginseng, the expression level of the ginseng PgWRKY2 transcription factor in the lateral root and the main root is the highest, and the expression level of the ginseng PgWRKY2 transcription factor in the leaf and the stem is the lowest.
5. The application of the ginseng PgWRKY2 transcription factor to improvement of the stress resistance of ginseng under the stress of different exogenous phosphorus concentrations is characterized in that the ginseng PgWRKY2 transcription factor has the highest expression level at the concentration of 2.0mM, has the lower expression level at the concentration of 4.0mM and has the expression level relatively lower at the concentrations of 0mM, 0.5mM and 1.0mM, and the expression level increases from the concentration of 0mM to the concentration of 2.0mM along with the increase of the phosphorus concentration and shows a positive correlation trend.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210517926.2A CN115260299A (en) | 2022-05-12 | 2022-05-12 | Ginseng PgWRKY2 transcription factor and application thereof |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202210517926.2A CN115260299A (en) | 2022-05-12 | 2022-05-12 | Ginseng PgWRKY2 transcription factor and application thereof |
Publications (1)
Publication Number | Publication Date |
---|---|
CN115260299A true CN115260299A (en) | 2022-11-01 |
Family
ID=83760155
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202210517926.2A Pending CN115260299A (en) | 2022-05-12 | 2022-05-12 | Ginseng PgWRKY2 transcription factor and application thereof |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN115260299A (en) |
Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013183961A1 (en) * | 2012-06-07 | 2013-12-12 | 강원대학교산학협력단 | Protopanaxatriol biosynthesis gene and promoting composition |
US20180319852A1 (en) * | 2017-05-02 | 2018-11-08 | Intelligent Synthetic Biology Center | Increased production of ginsenosides through yeast cell organelle improvement |
CN110607316A (en) * | 2019-08-23 | 2019-12-24 | 兰州理工大学 | Adversity stress response related gene in Lycium ruthenicum Murr, and encoding protein and cloning method thereof |
CN111217896A (en) * | 2020-02-28 | 2020-06-02 | 天津大学 | Application of ginseng PgWRKY4X transcription factor in regulating and controlling ginsenoside compound content in ginseng |
CN111909252A (en) * | 2020-09-25 | 2020-11-10 | 中国农业科学院特产研究所 | Ginseng PgbHLH149 transcription factor and application thereof |
CN112625103A (en) * | 2021-01-20 | 2021-04-09 | 上海交通大学 | Alfalfa WRKY transcription factor and application thereof in aluminum toxicity and salt stress resistance |
CN113234734A (en) * | 2021-03-22 | 2021-08-10 | 成都大学 | Sweet orange gene CsMYB30 capable of improving plant resistance and application thereof |
-
2022
- 2022-05-12 CN CN202210517926.2A patent/CN115260299A/en active Pending
Patent Citations (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2013183961A1 (en) * | 2012-06-07 | 2013-12-12 | 강원대학교산학협력단 | Protopanaxatriol biosynthesis gene and promoting composition |
US20180319852A1 (en) * | 2017-05-02 | 2018-11-08 | Intelligent Synthetic Biology Center | Increased production of ginsenosides through yeast cell organelle improvement |
CN110607316A (en) * | 2019-08-23 | 2019-12-24 | 兰州理工大学 | Adversity stress response related gene in Lycium ruthenicum Murr, and encoding protein and cloning method thereof |
CN111217896A (en) * | 2020-02-28 | 2020-06-02 | 天津大学 | Application of ginseng PgWRKY4X transcription factor in regulating and controlling ginsenoside compound content in ginseng |
CN111909252A (en) * | 2020-09-25 | 2020-11-10 | 中国农业科学院特产研究所 | Ginseng PgbHLH149 transcription factor and application thereof |
CN112625103A (en) * | 2021-01-20 | 2021-04-09 | 上海交通大学 | Alfalfa WRKY transcription factor and application thereof in aluminum toxicity and salt stress resistance |
CN113234734A (en) * | 2021-03-22 | 2021-08-10 | 成都大学 | Sweet orange gene CsMYB30 capable of improving plant resistance and application thereof |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN112375764B (en) | Fruit low-acid regulatory gene MdMYB44 and application thereof | |
CN104946665B (en) | GmMYB62Application in cultivating transgenic plant with improved stress resistance | |
CN110872598B (en) | Cotton drought-resistant related gene GhDT1 and application thereof | |
CN111235165B (en) | Lily susceptible fungal gene LrWRKY-S1 and application thereof | |
CN112608928B (en) | Longan single fruit weight character regulatory gene DlCNR8, protein and application thereof | |
CN113025626B (en) | Application of tumorous stem mustard BjuEAR1 gene in regulation of plant stress resistance | |
CN113337635A (en) | Chinese wolfberry gene and its coding protein, recombinant vector and use | |
CN111944030B (en) | Wheat stress resistance regulatory protein TaCOR58 and coding gene and application thereof | |
CN113831397A (en) | Proanthocyanidins substance regulatory factor NtMYB330, and expression vector, transformant, kit and method thereof | |
CN112175965A (en) | Gene and protein for enhancing resistance of rice blast and bacterial leaf blight and method for improving resistance of rice blast and bacterial leaf blight | |
CN109486831B (en) | Carmine radish anthocyanin biosynthesis regulatory gene RsAN1 and application thereof | |
CN108517324B (en) | NtIPMD gene affecting tobacco axillary bud differentiation | |
CN111718940A (en) | Sequence of carrot exogenous hormone-responsive related DcWRKY69 gene and application thereof | |
CN112626069A (en) | Soybean gma-miR4359b gene, expression vector thereof, preparation method and application thereof | |
CN115927311B (en) | China rose root specific expression promoter proRcbHLH120 and application thereof | |
CN115260299A (en) | Ginseng PgWRKY2 transcription factor and application thereof | |
CN111763685A (en) | PSY2 gene sequence related to synthesis of carrot carotenoid and application thereof | |
CN111394364B (en) | Zinc finger protein gene, synthesis method and application | |
CN114507674A (en) | Application of tea tree circadian rhythm gene LUX in improving cold resistance of plants | |
CN111217898B (en) | Application of protein OsZZW1 in regulation and control of drought resistance of rice | |
CN110004159B (en) | Key gene TcNAC1 for regulating salt tolerance of tamarix chinensis and application thereof | |
CN113136398A (en) | Application of GsA 24 protein and related biological material thereof in regulation and control of plant stress tolerance | |
CN106967732B (en) | Cotton glutathione peroxidase GhGPX8 and application thereof | |
CN111718944A (en) | Peanut salt-tolerant gene and application thereof | |
CN111808926A (en) | Wheat ear branch gene DNA sequence amplification method, branch gene and application |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination |