CN115251263A - Mulberry and rhizoma polygonati composite herbal enzyme capable of reducing high blood pressure, high blood sugar and preparation method thereof - Google Patents
Mulberry and rhizoma polygonati composite herbal enzyme capable of reducing high blood pressure, high blood sugar and preparation method thereof Download PDFInfo
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- YCPOHTHPUREGFM-UHFFFAOYSA-N irbesartan Chemical compound O=C1N(CC=2C=CC(=CC=2)C=2C(=CC=CC=2)C=2[N]N=NN=2)C(CCCC)=NC21CCCC2 YCPOHTHPUREGFM-UHFFFAOYSA-N 0.000 description 1
- 229960002198 irbesartan Drugs 0.000 description 1
- 229940054346 lactobacillus helveticus Drugs 0.000 description 1
- 230000001050 lubricating effect Effects 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- XZWYZXLIPXDOLR-UHFFFAOYSA-N metformin Chemical compound CN(C)C(=N)NC(N)=N XZWYZXLIPXDOLR-UHFFFAOYSA-N 0.000 description 1
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- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000005985 organic acids Nutrition 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
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- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 238000010926 purge Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 229960002855 simvastatin Drugs 0.000 description 1
- RYMZZMVNJRMUDD-HGQWONQESA-N simvastatin Chemical compound C([C@H]1[C@@H](C)C=CC2=C[C@H](C)C[C@@H]([C@H]12)OC(=O)C(C)(C)CC)C[C@@H]1C[C@@H](O)CC(=O)O1 RYMZZMVNJRMUDD-HGQWONQESA-N 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- ZSJLQEPLLKMAKR-GKHCUFPYSA-N streptozocin Chemical compound O=NN(C)C(=O)N[C@H]1[C@@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O ZSJLQEPLLKMAKR-GKHCUFPYSA-N 0.000 description 1
- 239000005720 sucrose Substances 0.000 description 1
- 235000021147 sweet food Nutrition 0.000 description 1
- 230000035488 systolic blood pressure Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- XPFJYKARVSSRHE-UHFFFAOYSA-K trisodium;2-hydroxypropane-1,2,3-tricarboxylate;2-hydroxypropane-1,2,3-tricarboxylic acid Chemical compound [Na+].[Na+].[Na+].OC(=O)CC(O)(C(O)=O)CC(O)=O.[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O XPFJYKARVSSRHE-UHFFFAOYSA-K 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
- A23L2/382—Other non-alcoholic beverages fermented
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Mycology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides a mulberry and rhizoma polygonati compound herbal enzyme capable of reducing high blood pressure, high blood sugar and high blood lipid and a preparation method thereof. The preparation method comprises the following steps: obtaining medicinal materials; cleaning and decocting the medicinal materials to extract soluble components in the medicinal materials, filtering to remove dregs, and sequentially inoculating lactic acid bacteria, saccharomycetes and acetic acid bacteria to filtrate obtained by filtering to perform multi-channel composite fermentation; standing the fermented mixed solution, taking supernatant, filtering, and blending the filtered supernatant. According to the invention, the medicinal materials are decocted and filtered to obtain the filtrate, and the filtrate is used for a subsequent process for preparing the ferment, so that the original medicinal materials are processed in advance, the use is convenient, and furthermore, the lactic acid bacteria, the saccharomycetes and the acetic acid bacteria are inoculated into the filtrate obtained by filtering to perform multi-channel composite fermentation, so that the concentration of the taste of the Chinese medicines is reduced, the bitterness of the fermented feed liquid is reduced, and the drinkability and the commodity value of the ferment are improved.
Description
Technical Field
The invention relates to the technical field of health-care beverages, and in particular relates to a mulberry, rhizoma polygonati and mulberry composite herbal enzyme capable of reducing high blood pressure, high blood sugar and high blood lipid and a preparation method thereof.
Background
For the control of three highs, the most effective means is medicine, and the hyperlipemia patients can take the medicine for reducing blood fat, such as simvastatin, atorvastatin, xuezhikang, etc. The hypertension patients can take captopril, irbesartan, amlodipine and other medicaments. Patients with hyperglycemia can take glipizide and metformin, and can also inject insulin. And then adding diet control, for example, the total amount of salt is not more than 6 g per day, the food with high fat content is required to be eaten less, the sweet food is required to be eaten less, the diabetic patients are required to limit the intake of the staple food, and the staple food is not more than 250g per day.
The herbal ferment is a fermented beverage which is prepared by taking medicinal and edible traditional Chinese medicinal materials as raw materials and fermenting the raw materials by microorganisms and has high-content active ingredients such as organic acid, polyphenol, amino acid, vitamin, polysaccharide, polyphenol and microorganism metabolites. Because the components have the effects of resisting oxidation, supplementing nutrient substances of human bodies and improving the health of the human bodies, the products containing the ferment are quite popular with consumers in the market at present.
Long-term traditional Chinese medicine experiments and researches in China show that many traditional Chinese medicines can achieve the effect of reducing high blood pressure, high blood fat and atherosclerosis, namely, the traditional Chinese medicines comprise cassia tora, hawthorn, dried orange peel, ginkgo leaf, medlar, chrysanthemum and the like; the Chinese medicinal materials for lowering blood pressure include radix Stephaniae Tetrandrae, radix Puerariae, fructus crataegi, flos Chrysanthemi Indici, herba Catharanthi rosei, fructus forsythiae, prunellae Spica, etc.; the Chinese medicinal materials for reducing blood sugar comprise Ginseng radix, radix astragali, poria, atractylodis rhizoma, rhizoma Atractylodis, rhizoma Dioscoreae, rhizoma Polygonati, rehmanniae radix, radix Ophiopogonis, etc. Because part of the traditional Chinese medicinal materials have high toxicity and bitter taste, the traditional Chinese medicinal materials have poor utilization rate after entering human bodies, and the traditional Chinese medicines are not convenient to drink after being decocted. In addition, most of the enzyme beverage products on the market are prepared by natural fermentation, so that the effective ingredients cannot be fully dissolved out, raw material waste is easily caused, and other microbial contamination is easily caused. In addition, the natural fermentation period of the ferment is very long, generally 2-4 months, and cannot meet the large demand of the market.
Disclosure of Invention
Based on the above, the invention aims to provide the mulberry and rhizoma polygonati compound herbal enzyme for reducing the three highs and the preparation method thereof, and the mulberry and rhizoma polygonati compound herbal enzyme is used for solving the technical problems that the traditional Chinese medicine for reducing the three highs is required to be decocted for drinking so as to be inconvenient to use, the traditional Chinese medicine is bitter in taste and poor in taste, and the natural fermentation period of the enzyme beverage is long in the prior art.
The invention provides a preparation method of a mulberry and rhizoma polygonati compound herbal enzyme for reducing hypertension, hyperglycemia and hyperlipidemia, which comprises the following steps:
obtaining medicinal materials, wherein the medicinal materials comprise longan pulp, kudzu root, mulberry, dark plum, orange peel, rhizoma polygonati, hawthorn, fructus alpiniae oxyphyllae, momordica grosvenori, poria cocos, red dates, medlar, liquorice and soybeans;
cleaning and decocting the medicinal materials to extract soluble components in the medicinal materials, filtering to remove residues, and sequentially inoculating lactic acid bacteria, yeast and acetic acid bacteria to filtrate obtained by filtering to perform multi-channel compound fermentation;
standing the fermented mixed solution, taking supernatant, filtering, and blending the filtered supernatant.
In addition, the preparation method of the invention can also have the following additional technical characteristics:
further, in the step of cleaning and decocting the medicinal material to extract the soluble components in the medicinal material, the decocting is: mixing the medicinal materials, adding water according to a material-liquid ratio of 1; and adding water according to the material-liquid ratio of 1.
Further, in the step of sequentially inoculating lactic acid bacteria, yeast and acetic acid bacteria into the filtrate obtained by filtration to perform multi-channel composite fermentation, the multi-channel composite fermentation includes lactic acid bacteria fermentation, and the lactic acid bacteria include, but are not limited to, any one or more of bifidobacterium adolescentis, bifidobacterium lactis, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, bifidobacterium longum, lactobacillus acidophilus, lactobacillus casei, lactobacillus crispatus, lactobacillus bulgaricus, lactobacillus delbrueckii subspecies lactis, lactobacillus fermentum, lactobacillus gasseri, lactobacillus helveticus, lactobacillus johnsonii, lactobacillus paracasei, lactobacillus plantarum, lactobacillus reuteri, lactobacillus rhamnosus, lactobacillus salivarius or streptococcus thermophilus; the fermentation temperature of the lactic acid bacteria is 41-43 ℃, the fermentation time is 48-72h, and the fermentation requirements are as follows: fermenting until the acidity is above 3 g/L.
Further, in the step of sequentially inoculating lactic acid bacteria, yeast and acetic acid bacteria into the filtrate obtained by filtration to perform multi-channel composite fermentation, the multi-channel composite fermentation includes yeast fermentation, and the yeast includes but is not limited to saccharomyces cerevisiae; the fermentation temperature of the yeast is 25-30 ℃, the fermentation time is 118-122h, and the fermentation requirement is as follows: circulating for 3 times every day until the alcoholic strength after fermentation is 1.5-2 degree.
Further, in the step of sequentially inoculating lactic acid bacteria, yeast and acetic acid bacteria to the filtrate obtained by filtration to perform multi-pass complex fermentation, the multi-pass complex fermentation includes acetic acid bacteria fermentation, and the acetic acid bacteria include but are not limited to any one or more of acetobacter pasteurianus, chinese family AS1.41 or Shanghai brewing 1.01; the fermentation temperature of the acetic acid bacteria is 29-33 ℃, the fermentation time is 70-74h, the ventilation per minute during fermentation is 0.1 time of the feed liquid, and the fermentation requirements are as follows: fermenting until the acidity is above 15 g/L.
Further, in the step of blending the filtered supernatant, the blending method comprises the following steps:
obtaining and dissolving white granulated sugar and fish collagen peptide, and transferring the dissolved white granulated sugar and fish collagen peptide into a blending tank;
adding the filtered supernatant into the blending tank, adding the concentrated blueberry juice and the concentrated cranberry juice, stirring until the blueberry juice and the concentrated cranberry juice are uniformly mixed, and heating the blending tank to sterilize to obtain the mulberry and rhizoma polygonati compound herbal ferment capable of reducing the hypertension, the hyperglycemia and the hyperlipidemia.
Further, in the step of heating the preparation tank to sterilize:
heating the blending tank at 90-95 deg.C for 10-15min.
Further, in the step of standing the fermented mixed solution, taking the supernatant and filtering, the filtering is: the supernatant was filtered using a celite filter and a plate and frame filter in this order.
The invention also provides a mulberry and rhizoma polygonati compound herbal enzyme for reducing three highs prepared by the preparation method, which comprises the following components in parts by weight:
4 parts of longan pulp; 2 parts of kudzu root; 1 part of mulberry; 1 part of dark plum; 2 parts of orange peel; 1 part of rhizoma polygonati; 1 part of hawthorn; 1 part of fructus alpiniae oxyphyllae; 1 part of momordica grosvenori; 1 part of poria cocos; 1 part of red dates; 1 part of medlar; 0.5 part of liquorice; 2 parts of soybean;
20 parts of water and 70 parts of white granulated sugar; 30 parts of fish collagen peptide;
concentrating 5 parts of blueberry juice; 5 parts of concentrated cranberry juice;
8 parts of brown granulated sugar; fructo-oligosaccharide, lactobacillus, yeast and acetic acid bacteria 1 part respectively.
Compared with the prior art, the invention has the following beneficial effects:
the invention provides a preparation method of a mulberry and rhizoma polygonati compound herbal enzyme for reducing hypertension, hyperlipidemia and hyperglycemia, which comprises the steps of decocting medicinal materials, filtering to obtain filtrate, and using the filtrate in a subsequent process for preparing the enzyme, so that the original medicinal materials are treated in advance, inconvenience of decoction during the use of the traditional method is avoided, and the traditional method is convenient to use; further, lactic acid bacteria, saccharomycetes and acetic acid bacteria are sequentially inoculated in the filtrate obtained by filtering to perform multi-channel composite fermentation, and in the microbial fermentation process, on one hand, the original traditional Chinese medicine odor can be converted into fermentation odor, so that the concentration of the traditional Chinese medicine odor is reduced, and on the other hand, the medicine components in the traditional Chinese medicine can be converted by the microorganisms, so that the bitterness of the fermented feed liquid is reduced, and the sourness is enhanced; on the other hand, the action of the microorganism changes the structure of the active ingredients of the traditional Chinese medicinal materials in the feed liquid, reduces the molecular size of the active ingredients, improves the activity of the active ingredients, enhances the activity of the active ingredients and improves the bioavailability; moreover, the filtered supernatant is blended, so that the supernatant has the effects of reducing the three highs of the traditional Chinese medicines, has the effects of resisting oxidation and maintaining beauty, improves the drinkability and the commodity value of the ferment for reducing the three highs, and solves the technical problems that the traditional Chinese medicines are inconvenient to use when being decocted for drinking and the traditional Chinese medicines are bitter in taste and poor in taste, and the ferment drink has long natural fermentation period in the prior art.
Detailed Description
The present invention will be described in further detail with reference to specific examples, which are not intended to limit the present invention, but to illustrate the present invention. The experimental methods used in the following examples are not specifically described, and the materials, reagents and the like used in the following examples are generally commercially available under the usual conditions without specific descriptions.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Terms and explanations:
the herbal enzyme is a fermented beverage which is prepared by taking medicinal and edible traditional Chinese medicinal materials as raw materials and performing microbial fermentation, has high-content active ingredients such as organic acids, polyphenols, amino acids, vitamins, polysaccharides, polyphenols and microbial metabolites, and has the effects of resisting oxidation, supplementing nutrient substances of human bodies and improving the health of human bodies. The drink prepared by combining the traditional Chinese medicines and the ferment can meet the requirements of human bodies on the ferment, has various tastes and can meet the health care requirements of the human bodies. If the traditional vegetable and fruit enzyme is supplemented with medicinal and edible traditional Chinese medicinal materials and the traditional Chinese medicinal materials are decomposed, extracted and converted by virtue of a microbial fermentation process, the effect of the enzyme is enhanced, and the health concept of herbaceous health preservation of the public at present is also met.
The selected traditional Chinese medicinal materials of longan pulp, kudzu vine root, mulberry, dark plum, orange peel, rhizoma polygonati, hawthorn, fructus alpiniae oxyphyllae, fructus momordicae, poria cocos, red dates, medlar and liquorice belong to the catalogue of homology of medicine and food. The traditional Chinese medicinal materials are mild in medicinal property and basically non-toxic, and compared with other traditional Chinese medicinal materials, the traditional Chinese medicinal materials can be suitable for long-term eating by people at the age stage, and have a history of being used for eating common foods in China for a long time. Therefore, the food prepared from the traditional Chinese medicinal materials has high safety and is harmless to the body.
The theoretical sources of the traditional Chinese medicine are as follows: according to records in pharmacopoeia, longan pulp has the functions of tonifying heart and spleen, nourishing blood and tranquillizing mind, hawthorn has the functions of promoting digestion and invigorating stomach, promoting the circulation of qi and dissipating blood stasis, dissolving turbidity and reducing blood fat, expelling pathogenic factors from muscles and skin, allaying fever, promoting the production of body fluid to quench thirst, mulberry has the functions of nourishing yin and enriching blood, promoting blood circulation and removing blood stasis, promoting the production of body fluid to quench thirst, releasing muscles and invigorating yang, nourishing yin and enriching blood, and relaxing bowel. The medlar has the effects of nourishing liver and kidney, benefiting vital essence, clearing heat, moistening lung, lubricating intestine and relaxing bowels by fructus momordicae, warming kidney and spleen by fructus alpiniae oxyphyllae, tonifying spleen by rhizoma polygonati, moistening lung and tonifying kidney, and has the effects of warming liver and kidney, tonifying heart and spleen, promoting fluid production to quench thirst, warming kidney and tonifying yang by combining the four traditional Chinese medicines. The red dates have the effects of tonifying middle-jiao and Qi, nourishing blood and soothing nerves, the poria cocos has the effects of promoting diuresis and excreting dampness, strengthening spleen, calming heart, the dried orange peel has the effects of regulating qi and strengthening spleen, eliminating dampness and reducing phlegm, and the red dates are combined with the soybeans to achieve the effects of tonifying middle-jiao and Qi, nourishing blood and soothing nerves, strengthening spleen and promoting diuresis, and regulating qi and reducing phlegm. Licorice root, radix Glycyrrhizae is effective in clearing away heat and toxic materials, harmonizing the effects of the above drugs, astringing lung, and promoting fluid production. The combination has the functions of warming and nourishing liver and kidney, tonifying heart and spleen, releasing muscles and raising yang, nourishing blood and tranquillizing, promoting the production of body fluid and quenching thirst, nourishing kidney and tonifying spleen qi, strengthening spleen and promoting diuresis, regulating qi and reducing phlegm, purging intense heat and removing toxicity, and has the functions of reducing fat, reducing blood pressure, reducing blood sugar and enhancing immunity.
Example 1
A preparation method of mulberry and rhizoma polygonati compound herbal enzyme for reducing high blood pressure, high blood sugar and high blood sugar comprises the following steps:
(1) Obtaining medicinal materials including 4 parts of longan pulp; 2 parts of kudzu root; 1 part of mulberry; 1 part of dark plum; 2 parts of orange peel; 1 part of rhizoma polygonati; 1 part of haw flakes; 1 part of fructus alpiniae oxyphyllae; 1 part of momordica grosvenori; 1 part of poria cocos; 1 part of red dates; 1 part of medlar; 0.5 part of liquorice; 2 parts of soybean;
(2) Cleaning and decocting medicinal materials to extract soluble components in the medicinal materials, wherein the decocting comprises the following specific steps: mixing the medicinal materials, adding water according to a material-liquid ratio of 1; adding water according to the material-liquid ratio of 1;
(3) Filtering the extracting solution obtained in the step (2), adding lactobacillus into the filtrate obtained by filtering for primary fermentation at 42 ℃ for 48-72 hours, and stopping when the acidity after fermentation is more than 3 g/L; adding yeast for secondary fermentation at 28 deg.C for 120 hr, and circulating for 3 times every day during the secondary fermentation until the alcoholic strength after fermentation is 1.5%; adding acetic acid bacteria after the secondary fermentation for carrying out tertiary fermentation, wherein the temperature of the tertiary fermentation is 30 ℃, the fermentation time is 72 hours, gas needs to be introduced in the process of the tertiary fermentation, the ventilation per minute is 0.1 time of the liquid feed amount, and the fermentation is stopped when the acidity is over 15 g/L; standing the mixed solution after the third fermentation, taking supernatant liquor and filtering to obtain filtered supernatant liquor;
(4) Obtaining and dissolving 70 parts of white granulated sugar and 30 parts of fish collagen peptide, and transferring the dissolved white granulated sugar and fish collagen peptide into a blending tank;
(5) Adding the filtered supernatant into a blending tank, adding 5 parts of concentrated blueberry juice and 5 parts of concentrated cranberry juice, stirring until the mixture is uniformly mixed, and heating the blending tank for 10-15min to sterilize to obtain the mulberry and rhizoma polygonati compound herbal ferment for reducing the hypertension, the hyperglycemia and the hyperlipidemia.
Comparative example 1
A preparation method of herbal enzyme comprises the following steps:
(1) Obtaining medicinal materials including 4 parts of longan pulp; 2 parts of kudzu root; 1 part of mulberry; 1 part of dark plum; 2 parts of orange peel; 1 part of rhizoma polygonati; 1 part of hawthorn slices; 1 part of fructus alpiniae oxyphyllae; 1 part of momordica grosvenori; 1 part of poria cocos; 1 part of red dates; 1 part of medlar; 0.5 part of liquorice; 2 parts of soybean;
(2) Cleaning the medicinal materials, directly adding water, adding lactobacillus for primary fermentation at 42 deg.C for 48-72 hr, and stopping when the acidity is greater than 3 g/L; adding yeast to perform secondary fermentation, wherein the temperature of the secondary fermentation is 28 ℃, the fermentation time is 120h, the circulation is performed for 3 times every day in the process of the secondary fermentation until the alcoholic strength after the fermentation is 1.5 ℃, adding acetic acid bacteria to perform tertiary fermentation after the secondary fermentation, the temperature of the tertiary fermentation is 30 ℃, the fermentation time is more than 72h, introducing gas in the process of the tertiary fermentation, the ventilation per minute is 0.1 time of the feed liquid amount, and the fermentation is stopped when the acidity is more than 15 g/L; standing the mixed solution after the third fermentation, taking supernatant liquor and filtering to obtain filtered supernatant liquor;
(3) Obtaining and dissolving 70 parts of white granulated sugar and 30 parts of fish collagen peptide, and transferring the dissolved white granulated sugar and fish collagen peptide into a blending tank;
(4) And adding the filtered supernatant into a blending tank, adding 5 parts of concentrated blueberry juice and 5 parts of concentrated cranberry juice, stirring until the mixture is uniformly mixed, and heating the blending tank for 10-15min to sterilize to obtain the herbal ferment.
Comparative example 2
A preparation method of herbal enzyme comprises the following steps:
(1) Obtaining medicinal materials including 4 parts of longan pulp; 2 parts of kudzu root; 1 part of mulberry; 1 part of dark plum; 2 parts of orange peel; 1 part of rhizoma polygonati; 1 part of hawthorn slices; 1 part of fructus alpiniae oxyphyllae; 1 part of momordica grosvenori; 1 part of poria cocos; 1 part of red dates; 1 part of medlar; 0.5 part of liquorice; 2 parts of soybean;
(2) Cleaning and decocting medicinal materials to extract soluble components in the medicinal materials, wherein the decocting comprises the following specific steps: mixing the medicinal materials, adding water according to a material-liquid ratio of 1; adding water according to the material-liquid ratio of 1;
(3) Filtering the extracting solution obtained in the step (2), and adding lactic acid bacteria into the filtrate obtained by filtering for fermentation, wherein the fermentation temperature is 42 ℃, the fermentation time is more than 72 hours, and the fermentation acidity reaches 6.8g/L; standing the fermented mixed solution, taking supernatant liquor and filtering to obtain filtered supernatant liquor;
(4) Obtaining and dissolving 70 parts of white granulated sugar and 30 parts of fish collagen peptide, and transferring the dissolved white granulated sugar and fish collagen peptide into a blending tank;
(5) And adding the filtered supernatant into a blending tank, adding 5 parts of concentrated blueberry juice and 5 parts of concentrated cranberry juice, stirring until the two are uniformly mixed, and heating the blending tank for 10-15min to sterilize to obtain the herbal ferment.
The fermentation results of the above examples and comparative examples were measured and statistically summarized, and the results are shown in table 1 below.
TABLE 1 fermentation results of examples and comparative examples
The above results show that the preparation method of example 1 (the medicinal material is heated to extract the soluble components, and then is subjected to three-pass fermentation) has better extraction effect on the phenolic substances and more dissolved active ingredients than the preparation method of comparative example 1 (the medicinal material is not heated to extract the soluble components, and is directly subjected to three-pass fermentation). In addition, the preparation method of example 1 can produce higher acidity than comparative example 2 (fermentation using only lactic acid bacteria) by using three fermentations (lactic acid bacteria + yeast + acetic acid bacteria).
Test example 1
The three-high reducing mulberry and rhizoma polygonati composite herbal enzyme obtained in the embodiment 1 of the invention is used for carrying out the following tests to detect the three-high reducing effect.
1. Hypotensive test
SD rats with the weight of 300 +/-20 g and half of the weight of the SD rats are selected and fed with standard feed for one week to ensure that the animals adapt to the environment and observe whether adverse reactions exist. 40 SD rats were randomly divided into 4 groups, each male and female half.
Blank control group: feeding with common feed, and intragastrically irrigating with common water once a day;
hypertension model group: feeding high-fat high-sugar feed (51% common feed, 15% lard, 15% sucrose, 15% egg yolk powder and 4% salt), alternately drinking sugar saline (sugar 4%, salt 1%) and fructose (6%), and intragastrically irrigating once a day with common water;
enzyme control group: feeding with common feed, and performing intragastric lavage with ferment once a day, with dosage of 1mL/100g body weight;
enzyme preparation: feeding with high-fat high-sugar feed, alternately drinking sugar saline (sugar 4%, salt 1%) and fructose (6%), and intragastrically feeding with ferment once a day with dosage of 1mL/100g body weight;
the rats in each group were fed water ad libitum and blood pressure measurements were taken 8 weeks after feeding, as shown in Table 2.
TABLE 2 Effect of the enzyme of the present invention on blood pressure in rats after intragastric administration
Group of | Systolic pressure (mmHg) | Diastolic blood pressure (mmHg) |
Blank control group | 135.4±7.3 | 113.5±6.5 |
Hypertension model group | 176.6±9.1 | 140.2±8.3 |
Enzyme control group | 129.3±7.9 | 108.6±7.5 |
Enzyme preparation set | 146.5±8.2 | 126.5±7.7 |
As can be seen from Table 2, the ferment prepared by the invention has a certain effect of preventing rat hypertension induced by high-fat high-sugar feed.
2. Lipid and blood sugar lowering test
Selecting Kunming mice with the weight of (20 +/-2 g), after adaptively feeding the Kunming mice for one week, randomly extracting 10 mice of each half of males and females to serve as a normal control group, fasting the rest mice overnight, carrying out intraperitoneal injection on Streptozotocin (STZ) with a single high dose (60 mg/kg) for modeling, injecting citric acid-sodium citrate buffer solution with the same volume into the normal control group, after one week, fasting the mice for 6 hours, and carrying out tail vein micro-blood sampling to measure the blood sugar value of an animal model, wherein the mice with the weight of more than or equal to 16mmol/L are successful in modeling. The 20 successfully molded diabetic mice were randomly divided into two groups, each group was half male and female, and each group was fed with normal feed. The experimental group was administered by gavage (enzyme of example 1) daily in an amount of 1mL/100g of body weight, and the normal control group and the model group were administered with the same amount of physiological saline. After 8 weeks of continuous administration, the mice were fasted for 6 hours, blood was taken from the tail vein, centrifuged at 3500r/min for 10min, serum was separated, and the serum blood glucose, triglyceride (TG), and cholesterol (TC) concentrations were measured using each kit, as shown in Table 3.
TABLE 3 influence of the enzyme of the present invention on blood glucose and blood lipid in mice
Group of | Blood sugar (mmol/L) | TC(mmol/L) | TG(mmol/L) |
Normal control group | 5.33±0.36 | 2.62±0.34 | 0.87±0.10 |
Model set | 29.74±1.35 | 4.02±0.51 | 1.98±0.73 |
Experimental group | 16.25±2.39 | 3.17±0.32 | 1.38±0.33 |
As can be seen from Table 3, the enzyme prepared by the invention has a certain down-regulation effect on blood sugar and blood fat of drug-induced diabetic mice.
In conclusion, the ferment prepared by the invention has certain prevention and down-regulation effects on hypertension, hyperlipidemia and hyperglycemia, and meanwhile, the traditional Chinese medicine active extract with the effect of reducing hypertension, hyperlipidemia and hyperglycemia is compounded with the fruit and vegetable ferment, so that the traditional Chinese medicine is more easily accepted by the public, and through the decomposition effect of microorganisms, nutrient substances in various raw materials are more easily absorbed and utilized by human bodies, and the immunity can be improved, the hypertension, the hyperlipidemia and the hyperglycemia can be prevented, and the body can be strengthened after long-term drinking of the ferment.
Test example 2
The enzymes obtained in example 1 and comparative examples 1 and 2 of the present invention were subjected to DPPH radical scavenging test, hydroxyl radical scavenging test and ABTS radical scavenging test, respectively, and three parallel tests were performed for each group, and the results of the scavenging rates are shown in table 4.
TABLE 4 clearance of different enzymes
Group of | DPPH clearance (%) | Hydroxyl radical scavenging rate (%) | ABTS clearance (%) |
Example 1 | 52.3 | 68.7 | 46.8 |
Comparative example 1 | 38.9 | 50.5 | 40.3 |
Comparative example 2 | 42.8 | 52.0 | 39.8 |
As can be seen from the above table, compared to the ferment of comparative examples 1 and 2, the ferment of the present invention has better effects of removing DPPH, hydroxyl radical, ABTS radical, and has better antioxidant effect. In the comparative example 1, the raw materials of the medicinal materials are directly subjected to microbial fermentation without being subjected to extraction of soluble components, so that the oxidation resistance of the raw materials is influenced to a certain extent; in comparative example 2, although the Chinese medicinal material is extracted by soluble components, the active ingredients of the Chinese medicinal material cannot be completely decomposed and converted due to the fact that only one kind of bacteria is inoculated for fermentation, and therefore the oxidation resistance is also influenced to a certain extent.
In the description of the specification, reference to the description of "one embodiment," "some embodiments," "an example," "a specific example," or "some examples" or the like means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The above-mentioned embodiments only express several embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.
Claims (9)
1. A preparation method of a mulberry and rhizoma polygonati compound herbal enzyme capable of reducing high blood pressure, high blood sugar and high blood lipid is characterized by comprising the following steps:
obtaining medicinal materials including longan pulp, kudzu vine root, mulberry, dark plum, orange peel, rhizoma polygonati, hawthorn, sharpleaf galangal fruit, momordica grosvenori, poria cocos, red dates, medlar, liquorice and soybeans;
cleaning and decocting the medicinal materials to extract soluble components in the medicinal materials, filtering to remove dregs, and sequentially inoculating lactic acid bacteria, saccharomycetes and acetic acid bacteria to filtrate obtained by filtering to perform multi-channel composite fermentation;
standing the fermented mixed solution, taking supernatant, filtering, and blending the filtered supernatant.
2. The method for preparing according to claim 1, wherein in the step of washing and decocting the medicinal material to extract the soluble components in the medicinal material, the decocting is: mixing the medicinal materials, adding water according to a material-liquid ratio of 1; and adding water according to the material-liquid ratio of 1.
3. The method according to claim 1, wherein the multi-step complex fermentation comprises a step of sequentially inoculating lactic acid bacteria, yeast and acetic acid bacteria into the filtered filtrate to perform multi-step complex fermentation, wherein the lactic acid bacteria are one or more of bifidobacterium adolescentis, bifidobacterium lactis, bifidobacterium bifidum, bifidobacterium breve, bifidobacterium infantis, bifidobacterium longum, lactobacillus acidophilus, lactobacillus casei, lactobacillus crispatus, lactobacillus bulgaricus, lactobacillus delbrueckii subspecies lactis, lactobacillus fermentum, lactobacillus gasseri, lactobacillus johnsonii, lactobacillus paracasei, lactobacillus plantarum, lactobacillus reuteri, lactobacillus rhamnosus, lactobacillus salivarius or streptococcus thermophilus; the fermentation temperature of the lactic acid bacteria is 41-43 ℃, the fermentation time is 48-72h, and the fermentation requirements are as follows: fermenting to acidity of above 3 g/L.
4. The method according to claim 1, wherein in the step of sequentially inoculating lactic acid bacteria, yeast and acetic acid bacteria to the filtrate obtained by the filtration to perform the multi-pass complex fermentation, the multi-pass complex fermentation includes yeast fermentation, and the yeast is saccharomyces cerevisiae; the fermentation temperature of the yeast is 25-30 ℃, the fermentation time is 118-122h, and the fermentation requirement is as follows: circulating for 3 times every day until the alcoholic strength after fermentation is 1.5-2 degree.
5. The method according to claim 1, wherein in the step of sequentially inoculating lactic acid bacteria, yeast and acetic acid bacteria to the filtrate obtained by filtration to perform multi-pass complex fermentation, the multi-pass complex fermentation includes acetic acid bacteria fermentation, and the acetic acid bacteria is one or more of Acetobacter pasteurianus, chinese AS1.41 or Shanghai 1.01; the fermentation temperature of the acetic acid bacteria is 29-33 ℃, the fermentation time is 70-74h, the ventilation per minute during fermentation is 0.1 time of the feed liquid, and the fermentation requirement is as follows: fermenting until the acidity is above 15 g/L.
6. The method according to claim 1, wherein in the step of preparing the filtered supernatant, the step of preparing comprises the steps of:
obtaining and dissolving white granulated sugar and fish collagen peptide, and transferring the dissolved white granulated sugar and fish collagen peptide into a blending tank;
adding the filtered supernatant into the blending tank, adding the concentrated blueberry juice and the concentrated cranberry juice, stirring until the blueberry juice and the concentrated cranberry juice are uniformly mixed, and heating the blending tank to sterilize to obtain the mulberry and rhizoma polygonati compound herbal ferment capable of reducing the hypertension, the hyperglycemia and the hyperlipidemia.
7. The method for preparing as claimed in claim 6, characterized in that, in the step of heating the preparation tank for sterilization:
heating the blending tank at 90-95 deg.C for 10-15min.
8. The method according to claim 1, wherein the step of standing the fermented mixture, collecting the supernatant, and filtering comprises: the supernatant was filtered using a celite filter and a plate and frame filter in this order.
9. The mulberry and rhizoma polygonati compound herbal enzyme capable of reducing the three highs is prepared by the preparation method of any one of claims 1 to 8, and is characterized by comprising the following components in parts by weight:
4 parts of longan pulp; 2 parts of kudzu roots; 1 part of mulberry; 1 part of dark plum; 2 parts of orange peel; 1 part of rhizoma polygonati; 1 part of hawthorn; 1 part of fructus alpiniae oxyphyllae; 1 part of momordica grosvenori; 1 part of poria cocos; 1 part of red dates; 1 part of medlar; 0.5 part of liquorice; 2 parts of soybean;
20 parts of water and 70 parts of white granulated sugar; 30 parts of fish collagen peptide;
concentrating 5 parts of blueberry juice; 5 parts of concentrated cranberry juice;
8 parts of brown granulated sugar; fructo-oligosaccharide, lactobacillus, yeast and acetic acid bacteria 1 part respectively.
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CN111165798A (en) * | 2020-01-03 | 2020-05-19 | 江西仁仁健康产业有限公司 | Preparation method of composite fruit and vegetable enzyme and composite fruit and vegetable enzyme |
CN113598361A (en) * | 2021-08-11 | 2021-11-05 | 江西仁仁健康产业有限公司 | Herbal enzyme capable of improving human immunity and preparation method thereof |
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CN111165798A (en) * | 2020-01-03 | 2020-05-19 | 江西仁仁健康产业有限公司 | Preparation method of composite fruit and vegetable enzyme and composite fruit and vegetable enzyme |
CN113598361A (en) * | 2021-08-11 | 2021-11-05 | 江西仁仁健康产业有限公司 | Herbal enzyme capable of improving human immunity and preparation method thereof |
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