CN115250918A - Plant tissue culture device with shading mechanism - Google Patents

Plant tissue culture device with shading mechanism Download PDF

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Publication number
CN115250918A
CN115250918A CN202211034500.8A CN202211034500A CN115250918A CN 115250918 A CN115250918 A CN 115250918A CN 202211034500 A CN202211034500 A CN 202211034500A CN 115250918 A CN115250918 A CN 115250918A
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China
Prior art keywords
incubator
plant tissue
tissue culture
plate
box
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CN202211034500.8A
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CN115250918B (en
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范付华
丁贵杰
万云梅
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Guizhou University
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Guizhou University
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H4/00Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
    • A01H4/001Culture apparatus for tissue culture

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  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cell Biology (AREA)
  • Botany (AREA)
  • Environmental Sciences (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention discloses a plant tissue culture device with a shading mechanism, and relates to the technical field of plant tissue culture. The invention comprises an incubator, wherein two opposite inner walls of the incubator are respectively matched with a conveyor belt in a rotating way, a plurality of cross rods are matched between the two conveyor belts in a rotating way, and balancing weights are fixed on the lower sides of the cross rods; the top of incubator is installed led lamp and electric putter, normal running fit has two V-arrangement baffles, normal running fit has the connecting rod between two V-arrangement baffles, and the connecting rod is installed on electric putter's output. The bottom of the culture bottle is conveniently limited through the action of the limiting groove, the culture bottle placed in the limiting groove is conveniently clamped and limited through the action of the arc-shaped clamping plate movably matched on one side of the supporting plate, the belt pulley is conveniently driven to rotate through the action of the motor, and the belt pulley drives the conveying belt to rotate, so that the positions of the cross rods on one side of the observation window can be adjusted in the rotating process of the conveying belt.

Description

Plant tissue culture device with shading mechanism
Technical Field
The invention belongs to the technical field of plant tissue culture, and particularly relates to a plant tissue culture device with a shading mechanism.
Background
Plant tissue culture is a technique of culturing isolated plant organs, tissues, cells and protoplasts in an aseptic and artificially controlled environment by using a proper culture medium to regenerate the cells or the whole plants. Also known as plant isolated culture. The whole process is aseptic operation, the culture condition can be artificially controlled, and the annual culture, research and production are convenient to carry out. Short growth period, high propagation rate, convenient management and contribution to industrial production and automatic control.
Because the plant materials adopted by the tissue culture completely grow under the culture medium and the climatic environment condition which are provided by people, different plants need different illumination conditions in the culture process, and the shading angle needs to be adjusted for different plants.
The angle of the inconvenient regulation shading of in-process that current incubator was using, the condition of plant tissue culture in inconvenient people's observation incubator simultaneously influences the control of plant tissue culture and then influences plant tissue culture's effect.
Disclosure of Invention
The invention aims to provide a plant tissue culture device with a shading mechanism, which solves the technical problem that the shading angle of the existing incubator is inconvenient to adjust in the using process.
In order to solve the technical problems, the invention is realized by the following technical scheme:
a plant tissue culture device with a shading mechanism comprises an incubator, wherein two opposite inner walls of the incubator are respectively matched with a conveyor belt in a rotating manner, a plurality of transverse rods are matched between the two conveyor belts in a rotating manner, the lower sides of the transverse rods are respectively fixed with a balancing weight, the upper sides of the transverse rods are respectively provided with a limiting groove and two fixing plates, the two fixing plates are symmetrically distributed on two sides of the limiting groove, one side of each fixing plate is elastically matched with a supporting plate, two sliding columns and two springs are arranged between the supporting plate and the fixing plates, one side of the supporting plate is elastically matched with an arc-shaped clamping plate, a culture bottle is arranged between the two arc-shaped clamping plates, and the bottom of the culture bottle is positioned in the limiting groove;
the two sides of the culture bottle are respectively provided with a light screen, the light screens are fixed on the upper sides of the cross rods, the arc-shaped clamping plate is positioned between the two light screens, a shielding plate and a culture medium are arranged in the culture bottle, the shielding plate is positioned on the upper side of the culture medium, and plant culture tissues are arranged on the upper side of the shielding plate;
the top of the incubator is provided with an LED lamp and an electric push rod, two V-shaped baffles are in running fit, a connecting rod is in running fit between the two V-shaped baffles, the connecting rod is arranged on the output end of the electric push rod, one end of each V-shaped baffle is fixed with an anti-collision plate, and one side of each anti-collision plate is provided with an anti-collision strip;
one side normal running fit of incubator has the chamber door, is equipped with the observation window on the chamber door, and one side of observation window is equipped with the shielding curtain.
Optionally, the inner wall of the incubator is in running fit with two belt pulleys, and the transmission belt is matched on the peripheral sides of the two belt pulleys.
Optionally, a rotating shaft is arranged between the belt pulley and the incubator, and a connecting rod is fixed between the two opposite belt pulleys.
Optionally, a motor is arranged on one side of the incubator, and a rotating shaft is arranged at the output end of the motor.
Optionally, one end of the sliding column penetrates through and is in sliding fit with the fixing plate, and the spring is located on the periphery side of the sliding column.
Optionally, all be equipped with the connection mouth on two relative inner walls of incubator, the one end of connection mouth all is equipped with the shower nozzle, and the other end of connection mouth all is connected with the hose, and the one end of hose is connected with the humidifier.
Optionally, two door hinges are arranged between the box door and the incubator, and a sealing strip is fixed on the inner side of the box door.
Optionally, one side of the box door is provided with a rolling box, a clamping ring and two rails, the shielding curtain is rolled in the rolling box, the two sides of the shielding curtain are in sliding fit in the rails, and the lower side of the shielding curtain is provided with a buckle matched with the clamping ring.
Optionally, the lower side of the incubator is provided with four support legs, and the four support legs are respectively located at four corners of the lower side of the incubator.
Optionally, a screw rod is matched with the inner thread of the supporting leg, a base is movably matched with one end of the screw rod, and an anti-slip gasket is arranged on the lower side of the base.
The embodiment of the invention has the following beneficial effects:
according to one embodiment of the invention, the bottom of the culture bottle is conveniently limited through the action of the limiting groove, the arc-shaped clamping plate can be conveniently attached to the side wall of the culture bottle through the action of the spring, the arc-shaped clamping plate is conveniently attached to the side wall of the culture bottle through the action of the arc-shaped clamping plate in a movable fit manner on one side of the supporting plate, the culture bottle placed in the limiting groove is conveniently clamped and limited, the stability of the culture bottle on the cross rod is improved, the belt pulley is conveniently driven to rotate through the action of the motor, the belt pulley is further convenient to drive the conveying belts to rotate, the positions of a plurality of cross rods on one side of the observation window can be conveniently adjusted in the rotating process of the conveying belts, the cross rod can be conveniently rotated between two conveying belts through the action of the balancing weight in the process of adjusting the positions of the cross rods, the culture bottle is further enabled to be in an upright state all the time, the possibility of toppling of the culture bottle is reduced, the height of the connecting rod is conveniently adjusted through the action of the electric push rod, the V-shaped baffle can be conveniently driven to rotate between two supporting columns, the brightness generated by the LED lamp, the light is conveniently adjusted, the effect of the anti-collision of the culture bottle is further, and the observation window is improved.
Of course, it is not necessary for any product in which the invention is practiced to achieve all of the above-described advantages at the same time.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to the drawings without creative efforts.
FIG. 1 is a schematic perspective view of a front view according to an embodiment of the present invention;
FIG. 2 is a schematic view of the structure at A in FIG. 1;
FIG. 3 is a schematic view of an assembly structure of the curtain on one side of the door according to an embodiment of the present invention;
FIG. 4 is a schematic view of an assembly structure of a led lamp on the top of an incubator according to an embodiment of the present invention;
FIG. 5 is a schematic cross-sectional view illustrating an embodiment of the present invention;
FIG. 6 is a schematic view of an assembly structure of a culture flask on a cross bar according to an embodiment of the present invention;
FIG. 7 is a schematic view of the structure at B in FIG. 6;
FIG. 8 is a schematic cross-sectional view of a culture flask according to an embodiment of the present invention.
Wherein the figures include the following reference numerals:
the cultivation box comprises a cultivation box 1, a belt pulley 2, a conveyor belt 3, a cross rod 4, a limiting groove 5, a fixing plate 6, a sliding column 7, a spring 8, a supporting plate 9, an arc-shaped clamping plate 10, a motor 11, a connecting nozzle 12, a spray head 13, a fixing column 14, an LED lamp 15, a supporting column 16, a V-shaped baffle 17, an anti-collision strip 18, an electric push rod 19, a connecting rod 20, a supporting leg 21, a screw rod 22, a base 23, an anti-slip gasket 24, a door 25, a box door 26, a rolling box 27, a shielding curtain 28, a rail 29, a buckle 30, a clamping ring 31, a sealing strip 32, an anti-collision plate 33, a shielding plate 34, a cultivation medium 35, a cultivation bottle 36 and a light shielding plate 37.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. The following description of at least one exemplary embodiment is merely illustrative in nature and is in no way intended to limit the invention, its application, or uses.
To maintain the following description of the embodiments of the present invention clear and concise, a detailed description of known functions and known components of the invention have been omitted.
Referring to fig. 1-6, in the present embodiment, a plant tissue culture apparatus with a light shielding mechanism is provided, including an incubator 1, two opposite inner walls of the incubator 1 are both rotatably fitted with conveyor belts 3, a plurality of cross bars 4 are rotatably fitted between the two conveyor belts 3, the lower sides of the cross bars 4 are both fixed with balancing weights, the upper sides of the cross bars 4 are both provided with limiting grooves 5 and are provided with two fixing plates 6, and the two fixing plates 6 are symmetrically distributed at two sides of the limiting grooves 5, one side of the fixing plate 6 is elastically fitted with a support plate 9, two sliding posts 7 and two springs 8 are arranged between the support plate 9 and the fixing plate 6, one side of the support plate 9 is elastically fitted with arc-shaped clamping plates 10, a culture bottle 36 is arranged between the two arc-shaped clamping plates 10, and the bottom of the culture bottle 36 is located in the limiting groove 5;
the two sides of the culture bottle 36 are provided with the light shielding plates 37, the light shielding plates 37 are fixed on the upper side of the cross rod 4, the arc-shaped clamping plate 10 is located between the two light shielding plates 37, the shielding plate 34 and the culture medium 35 are arranged in the culture bottle 36, the shielding plate 34 is located on the upper side of the culture medium 35, plant culture tissues are arranged on the upper side of the shielding plate 34, specifically, holes are formed in the shielding plate 34, the plant tissues are convenient to contact with the culture medium 35 through the holes, and the root of the plant tissues are convenient to be shielded through the shielding plate 34 and the light shielding plates 37;
the top of the incubator 1 is provided with an LED lamp 15 and an electric push rod 19, the two V-shaped baffles 17 are in running fit, a connecting rod 20 is in running fit between the two V-shaped baffles 17, the connecting rod 20 is arranged at the output end of the electric push rod 19, one end of each V-shaped baffle 17 is fixed with an anti-collision plate 33, one side of each anti-collision plate 33 is provided with an anti-collision strip 18, specifically, two fixing columns 14 are arranged between the LED lamp 15 and the incubator 1, two ends of each V-shaped baffle 17 are provided with supporting columns 16, the supporting columns 16 are fixed on the incubator 1, and the V-shaped baffles 17 are in running fit with one side of the supporting columns 16;
a box door 26 is rotatably matched at one side of the incubator 1, an observation window is arranged on the box door 26, and a shielding curtain 28 is arranged at one side of the observation window.
The application of one aspect of the embodiment is as follows: in the using process of the culture device, the culture bottle 36 with plant tissues is placed on the limiting groove 5, then the supporting plate 9 pushes the arc-shaped clamping plate 10 to approach to the outer wall of the culture bottle 36 under the action of the spring 8, then the arc-shaped clamping plate 10 clamps the culture bottle 36 tightly, further the culture bottle 36 is limited on the upper side of the cross rod 4, then the box door 26 is closed to enable the box door 26 to hermetically store the culture bottle 36 in the culture box 1, then the electric push rod 19 drives the connecting rod 20 to move upwards, further the V-shaped baffle 17 is driven to rotate around the supporting column 16, the V-shaped baffle 17 can shield the led lamp 15, further the irradiation intensity of the led lamp 15 on the culture bottle 36 is realized, then when the condition of the culture bottle 36 in the culture box 1 needs to be observed, the electric push rod 19 can drive the two V-shaped baffles 17 to open, then the shielding curtain 28 is pulled open to enable a person to observe the observation window from the observation window, the condition of plant tissue culture is observed, then the belt pulley 2 is driven to rotate through the motor 11, the belt pulley 2 can drive the conveyor belt 3 to rotate, further the position of the shielding rod 4 is adjusted, the convenience is convenient for observing of the different plant tissues on the different observation window 4, and the observation of the culture bottle 36, and finally the observation window can observe the plant tissues again according to the illumination intensity of the culture bottle 15. It should be noted that all the electric devices referred to in this application may be powered by a storage battery or an external power source.
The bottom of the culture bottle 36 is conveniently limited under the action of the limiting groove 5, the arc-shaped clamping plate 10 can be conveniently and tightly attached to the side wall of the culture bottle 36 under the action of the spring 8, the arc-shaped clamping plate 10 is conveniently and integrally attached to the side wall of the culture bottle 36 under the action of the movable fit of the arc-shaped clamping plate 10 on one side of the supporting plate 9, the culture bottle 36 placed in the limiting groove 5 can be conveniently clamped and limited, the stability of the culture bottle 36 on the cross rod 4 is improved, the belt pulley 2 can be conveniently driven to rotate under the action of the motor 11, the belt pulley 2 can further drive the conveyor belt 3 to rotate, the positions of a plurality of cross rods 4 on one side of the observation window can be adjusted during the rotation process of the conveyor belt 3, the cross rod 4 can be conveniently rotated between the two conveyor belts 3 under the action of the balancing weight, and then make blake bottle 36 be in the state of standing vertically always, reduce the possibility that blake bottle 36 appears empting, the height of connecting rod 20 is conveniently adjusted to the effect through electric putter 19, and then the convenience can drive V-arrangement baffle 17 at the in-process that connecting rod 20 removed and rotate between two support columns 16, and then conveniently carry out the shading to the light that led lamp 15 produced, convenient two V-arrangement baffle 17 one end to the contact of buffering of effect through anticollision strip 18, reduce the possibility that two V-arrangement baffles 17 appear damaging in the use, conveniently observe the condition of blake bottle 36 in incubator 1 through the effect of observation window, and then conveniently adjust the shading luminance of V-arrangement baffle 17 to led lamp 15 according to the condition that the observation obtained, and then improve the effect of cultivateing plant tissue.
Please refer to fig. 1, the inner wall normal running fit of incubator 1 of this embodiment has two belt pulleys 2, conveyer belt 3 cooperates in the week side of two belt pulleys 2, be equipped with the pivot between belt pulley 2 and incubator 1, be fixed with the connecting rod between two relative belt pulleys 2, one side of incubator 1 is equipped with motor 11, a pivot dress is established on the output of motor 11, conveniently drive belt pulley 2 through the effect of motor 11 and rotate, conveniently drive another belt pulley 2 through the effect of connecting rod and rotate simultaneously, and then conveniently drive two conveyer belts 3 and rotate simultaneously, and then conveniently adjust the position of horizontal pole 4 in incubator 1.
Referring to fig. 2, one end of the sliding column 7 of the present embodiment penetrates through and is slidably fitted in the fixing plate 6, and the spring 8 is located on the periphery of the sliding column 7, so that the supporting plate 9 is conveniently supported by the action of the sliding column 7, and the supporting plate 9 is further conveniently moved on one side of the fixing plate 6.
Please refer to fig. 1 and show, all be equipped with on the relative two inner walls of incubator 1 of this embodiment and connect mouth 12, the one end of connecting mouth 12 all is equipped with shower nozzle 13, and the other end of connecting mouth 12 all is connected with the hose, and the one end of hose is connected with the humidifier, and the effect through the humidifier is convenient to carry out the humidification operation in to incubator 1 through connecting mouth 12, simultaneously through heating to the humidifier, conveniently improves the temperature in incubator 1.
Please refer to fig. 3, in the embodiment, two door hinges 25 are disposed between the box door 26 and the incubator 1, a sealing strip 32 is fixed on an inner side of the box door 26, a rolling box 27, a snap ring 31 and two rails 29 are disposed on one side of the box door 26, a shielding curtain 28 is rolled in the rolling box 27, two sides of the shielding curtain 28 are both slidably fitted in the rails 29, a buckle 30 fitted with the snap ring 31 is disposed on a lower side of the shielding curtain 28, the box door 26 is conveniently mounted on one side of the incubator 1 through the effect of the door hinges 25, a joint between the box door 26 and the incubator 1 is conveniently sealed through the effect of the sealing strip 32, the shielding curtain 28 is conveniently clamped on the buckle 30 through the effect of the snap ring 31, and is further convenient to fix the shielding curtain 28, the shielding curtain 28 is conveniently rolled through the effect of the rolling box 27, the unfolded shielding curtain 28 is conveniently limited through the effect of the rails 29, and the possibility of shaking of the shielding curtain 28 in the use process is reduced.
Please refer to fig. 1, the downside of incubator 1 of this embodiment is equipped with four supporting legs 21, and four supporting legs 21 are located four corners of incubator 1 downside respectively, supporting leg 21 female fit has lead screw 22, the one end clearance fit of lead screw 22 has base 23, the downside of base 23 is equipped with anti-skidding gasket 24, conveniently adjust the distance between anti-skidding gasket 24 and the ground through the effect of lead screw 22 screw-thread fit in supporting leg 21, make incubator 1 can the horizontally arrange subaerially in, through the effect of base 23 clearance fit in lead screw 22 one end, make things convenient for the laminating that anti-skidding gasket 24 can be inseparable subaerial, can provide skid-proof effect to incubator 1 placing subaerial through the effect of anti-skidding gasket 24.
The above embodiments may be combined with each other.
It should be noted that in the description of the present specification, descriptions such as "first", "second", etc. are only used for distinguishing features, and do not have actual sequence or directional meaning, and the present application is not limited thereto.
In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.
The preferred embodiments of the invention disclosed above are intended to be illustrative only. The preferred embodiments are not intended to be exhaustive or to limit the invention to the precise embodiments disclosed. Obviously, many modifications and variations are possible in light of the above teaching. The embodiments were chosen and described in order to best explain the principles of the invention and the practical application, to thereby enable others skilled in the art to best utilize the invention. The invention is limited only by the claims and their full scope and equivalents.

Claims (10)

1. A plant tissue culture device with a shading mechanism, comprising: the cultivation box comprises a cultivation box (1), wherein two opposite inner walls of the cultivation box (1) are respectively in running fit with a conveyor belt (3), a plurality of transverse rods (4) are respectively in running fit between the two conveyor belts (3), balancing weights are respectively fixed on the lower sides of the transverse rods (4), limiting grooves (5) are respectively formed in the upper sides of the transverse rods (4), two fixing plates (6) are respectively arranged, the two fixing plates (6) are symmetrically distributed on two sides of each limiting groove (5), a supporting plate (9) is elastically matched on one side of each fixing plate (6), two sliding columns (7) and two springs (8) are arranged between the supporting plate (9) and the fixing plate (6), arc-shaped clamping plates (10) are elastically matched on one side of the supporting plate (9), a cultivation bottle (36) is arranged between the two arc-shaped clamping plates (10), and the bottom of the cultivation bottle (36) is located in the limiting grooves (5);
the two sides of the culture bottle (36) are respectively provided with a light screen (37), the light screens (37) are fixed on the upper side of the cross rod (4), the arc-shaped clamping plate (10) is positioned between the two light screens (37), a shielding plate (34) and a culture medium (35) are arranged in the culture bottle (36), the shielding plate (34) is positioned on the upper side of the culture medium (35), and plant culture tissues are arranged on the upper side of the shielding plate (34);
the top of the incubator (1) is provided with an LED lamp (15) and an electric push rod (19), two V-shaped baffles (17) are in running fit, a connecting rod (20) is in running fit between the two V-shaped baffles (17), the connecting rod (20) is arranged at the output end of the electric push rod (19), one end of each V-shaped baffle (17) is fixed with an anti-collision plate (33), and one side of each anti-collision plate (33) is provided with an anti-collision strip (18);
one side normal running fit of incubator (1) has chamber door (26), be equipped with the observation window on chamber door (26), one side of observation window is equipped with blind curtain (28).
2. The plant tissue culture apparatus with a light shielding mechanism according to claim 1, wherein the inner wall of the incubator (1) is rotatably fitted with two pulleys (2), and the conveyor belt (3) is fitted around the periphery of the two pulleys (2).
3. The plant tissue culture device with the light shielding mechanism as claimed in claim 2, wherein a rotating shaft is provided between the belt pulley (2) and the incubator (1), and a connecting rod is fixed between the two opposite belt pulleys (2).
4. A plant tissue culture device with shading mechanism according to claim 3, characterized in that the incubator (1) is provided with a motor (11) on one side, and the rotating shaft is mounted on the output end of the motor (11).
5. A plant tissue culture apparatus with a light shading mechanism according to claim 1, wherein one end of the slide column (7) penetrates and is slidably fitted in the fixing plate (6), and the spring (8) is located on the peripheral side of the slide column (7).
6. The plant tissue culture device with the light shielding mechanism is characterized in that the two opposite inner walls of the culture box (1) are respectively provided with a connecting nozzle (12), one end of each connecting nozzle (12) is provided with a spray head (13), the other end of each connecting nozzle (12) is connected with a hose, and one end of each hose is connected with a humidifier.
7. The plant tissue culture device with the shading mechanism according to claim 1, wherein two door hinges (25) are arranged between the box door (26) and the incubator (1), and a sealing strip culture bottle (36) is fixed on the inner side of the box door (26).
8. The plant tissue culture device with the light shielding mechanism is characterized in that a rolling box (27), a clamping ring (31) and two rails (29) are arranged on one side of the box door (26), the shielding curtain (28) is rolled in the rolling box (27), two sides of the shielding curtain (28) are in sliding fit in the rails (29), and a buckle (30) matched with the clamping ring (31) is arranged on the lower side of the shielding curtain (28).
9. The plant tissue culture apparatus with a light shielding mechanism according to claim 1, wherein the lower side of the incubator (1) is provided with four support legs (21), and the four support legs (21) are respectively located at four corners of the lower side of the incubator (1).
10. The plant tissue culture device with the light shielding mechanism as claimed in claim 9, wherein the supporting leg (21) is internally threaded with a screw rod (22), one end of the screw rod (22) is movably fitted with a base (23), and the lower side of the base (23) is provided with an anti-slip gasket (24).
CN202211034500.8A 2022-08-26 2022-08-26 Plant tissue culture device with shading mechanism Active CN115250918B (en)

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Cited By (1)

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