CN115248319A

CN115248319A - POCT blood cell analyzer and using method thereof

Info

Publication number: CN115248319A
Application number: CN202110454620.2A
Authority: CN
Inventors: 谭玉华; 秦军芳; 梁铁柱
Original assignee: Shenzhen Dymind Biotechnology Co Ltd
Current assignee: Shenzhen Dymind Biotechnology Co Ltd
Priority date: 2021-04-26
Filing date: 2021-04-26
Publication date: 2022-10-28

Abstract

The application provides a use method of a POCT blood cell analyzer, which comprises the following steps: receiving a starting trigger signal; performing hardware self-check; initializing the whole machine; receiving the kit for loading; and carrying out automatic sample pretreatment on the sample on the kit and carrying out sample detection after the automatic sample pretreatment is finished.

Description

POCT blood cell analyzer and using method thereof

Technical Field

The application relates to the technical field of medical instruments, in particular to a POCT blood cell analyzer, a pipettor, a detection seat, a using method of the POCT blood cell analyzer, a kit, an assembly seat, a sample detection device and a microporous sheet.

Background

The hemocyte analyzer is called hemocyte analyzer, blood-ball meter, blood-cell counter, etc. and is one of the widely used instruments for clinical examination in hospital. Most parts of the components in the conventional blood cell analyzer belong to a cleaning system, because the use trace of the previous sample tube must be cleaned before the next sample tube detection. The whole cleaning system is complex in structure and multiple in parts, and a large amount of reagents are needed in the cleaning process, so that the cleaning system occupies a long time.

Compared with the traditional blood cell analyzer, the POCT blood cell analyzer is greatly simplified in instrument components, and the POCT blood cell analyzer can completely remove relevant components of a cleaning liquid path in the traditional blood analysis, so that the complexity and the production cost of the instrument are greatly reduced.

However, some conventional POCT hematology analyzers have too simple functions, complex structures, low automation degrees, and high costs.

Disclosure of Invention

The present application provides a POCT blood cell analyzer, a pipette, a detection holder, a method of using a POCT blood cell analyzer, a kit, an assembly holder, a sample detection device, and a microchip, to at least partially solve the above technical problems.

In order to solve the technical problem, the application adopts a technical scheme that: provided is a method for using a POCT blood cell analyzer, comprising: receiving a starting trigger signal; performing hardware self-check; initializing the whole machine; receiving the kit loading; and carrying out automatic sample pretreatment on the sample on the kit and carrying out sample detection after the automatic sample pretreatment is finished.

The beneficial effect of this application is: different from the prior art, the POCT blood cell analyzer, the pipettor, the detection seat, the using method of the POCT blood cell analyzer, the kit, the assembly seat, the sample detection device and the microchip provided by the application have the advantages of novel structure, practicability, reliability, low cost and capability of efficiently and completely performing automated detection.

Drawings

In order to more clearly illustrate the technical solutions in the embodiments, the drawings needed to be used in the description of the embodiments are briefly introduced below, it is obvious that the drawings in the following description are only some embodiments of the present application, and other drawings can be obtained by those skilled in the art without inventive efforts, wherein:

fig. 1 is a schematic perspective view of a POCT blood cell analyzer according to an embodiment of the present disclosure;

fig. 2 is a schematic block diagram of a POCT blood cell analyzer according to an embodiment of the present disclosure;

fig. 3 is a schematic diagram of an internal structure of a POCT blood cell analyzer according to an embodiment of the present application;

fig. 4 is a schematic diagram of an internal structure of a POCT blood cell analyzer according to an embodiment of the present application, in which a pipette is omitted;

fig. 5 is a schematic view of an air path structure of a pressure buildup system according to an embodiment of the present application;

fig. 6 is a schematic perspective view of a pipette provided in an embodiment of the present application;

fig. 7 is a schematic perspective view of a puncture head according to an embodiment of the present application;

fig. 8 is a schematic bottom view of a metal shielding cover and a transmission mechanism thereof of a POCT blood cell analyzer according to an embodiment of the present disclosure;

fig. 9 is a schematic perspective view of a detection seat of a POCT blood cell analyzer according to an embodiment of the present disclosure;

fig. 10 is a schematic diagram of an exploded structure of a detection seat of a POCT blood cell analyzer according to an embodiment of the present application;

fig. 11 is a schematic side view of a conductive holder and a conductive column of a POCT blood cell analyzer according to another embodiment of the present disclosure;

FIG. 12 is a schematic top view of a kit according to an embodiment of the present disclosure;

fig. 13 is a schematic diagram of a simplified perspective structure of a POCT blood cell analyzer according to another embodiment of the present application;

fig. 14 is a flow chart illustrating the use of the POCT blood cell analyzer according to an embodiment of the present disclosure;

FIG. 15 is a schematic view of an exploded view of a cartridge according to one embodiment of the present application;

FIG. 16 is a schematic exploded view of another perspective of a kit according to an embodiment of the present application;

FIG. 17 is a schematic cross-sectional view of a viewing angle of a kit according to an embodiment of the present disclosure;

FIG. 18 is a schematic cross-sectional view of another perspective of a kit according to an embodiment of the present disclosure;

FIG. 19 is a schematic perspective view of a microporous sheet according to an embodiment of the present application;

fig. 20 is a schematic cross-sectional structure of the microporous sheet shown in fig. 19.

Detailed Description

The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.

It should be noted that, if directional indications (such as up, down, left, right, front, and back … …) are involved in the embodiment of the present application, the directional indications are only used to explain the relative position relationship between the components, the motion situation, and the like in a specific posture (as shown in the drawing), and if the specific posture is changed, the directional indication is changed accordingly.

In addition, if there is a description of "first", "second", etc. in the embodiments of the present application, the description of "first", "second", etc. is for descriptive purposes only and is not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions between various embodiments may be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, such a combination should not be considered to exist, and is not within the protection scope of the present application.

The application provides a POCT blood cell analyzer and a using method thereof.

As shown in fig. 1 to 14, the POCT blood cell analyzer includes a housing 10, a display screen 11, a discharge port 12, a syringe 220, a frame 301, a pressure buildup system, a detection seat 300 and a transmission mechanism thereof, a pipette 400 and a transmission mechanism thereof, a metal shielding cover 500 and a transmission mechanism thereof, and the like.

The detection seat 300 can be in and out of the outlet 12 in a reciprocating manner so as to facilitate loading or taking out of the reagent kit 20, the pipette 400 and the transmission mechanism thereof are used for realizing full-automatic pretreatment of a sample to be detected (which can be pre-loaded on the reagent kit 20), the full-automatic pretreatment comprises automatic sample adding, automatic reagent adding (diluent, hemolytic agent, staining lysis reagent and the like are pre-loaded on the reagent kit 20), automatic mixing (bubbling or stirring) and the like, the pressure building system is used for providing positive pressure and negative pressure required by the pipette 400 for the full-automatic pretreatment, the metal shielding cover 500 and the transmission mechanism thereof are used for automatically detecting in cooperation with the pressure building system, the automatic detection means that when the metal shielding cover 500 and the detection seat 300 are covered to form an electromagnetic shielding space, the air pressure connecting device 510 of the pressure building system is communicated with the pressure action cavity 140 on the reagent kit 20 (the pressure action cavity 140 is communicated with the rear cell of the impedance detection cell), and the pressure chamber 210 of the pressure building system starts to provide the negative pressure or the positive pressure so that liquid in the front cell 120 of the impedance detection cell of the reagent kit 20 flows to the rear cell of the impedance detection cell through the microporous reagent kit 20 and records relevant parameters in the process. Wherein, the pneumatic connecting device 510 of the pressure building system is connected with the metal shielding cover 500 and moves synchronously.

The pressure building system comprises a pressure chamber 210, a first injector 222 and a second injector 223 which are arranged in a linkage mode, a driving piece 224, an air filter 225, a first solenoid valve SV1, a second solenoid valve SV2, a third solenoid valve SV3, a pneumatic connecting device 510 and the like.

The pipette 400 includes an air duct 401, an outer sleeve 402 sleeved on the periphery of the air duct 401, a synchronizing block 403, a light blocking sheet 404, an optical coupler 405, a fixing plate 406, a through slot 407, a stopper 4031 for unloading the mounting head (201 or 204), and the like.

The metal shielding cover 500 is mounted on the frame 301 through a mounting frame 520, and is lifted and lowered in the vertical direction through a motor 530 and a guide post 540, the metal shielding cover 500 is in a cover shape with an opening at the lower part, and an air pressure connecting device 510 connected with a pressure building system extends into the metal shielding cover 500.

The test socket 300 includes a main body 310, a conductive holder 303 connected to the main body 310, metal shielding sockets (320, 321, 322) covering the main body 310, an optical test component 330 embedded in the main body 310, a peltier 340, a heat sink 350, a fan 360, and the like, which are sequentially disposed near a test cavity of the optical test component 330.

Hereinafter, the POCT blood cell analyzer will be described in detail with reference to examples.

A first embodiment, as shown in fig. 1 to 13, provides a POCT blood cell analyzer, which includes a housing 10, a detection seat 300, and a pipette 400.

The test socket 300 may extend into or out of the housing 10 for receiving a reagent cartridge 20 provided with an impedance test cell, and the test socket 300 is provided with a power supply (e.g., a conductive holder 303 shown in fig. 9) for matching impedance test. The cartridge 20 has a mounting head receiving bay for mounting a mounting head (tip head 201 or piercing head 204).

The pipettor 400 is disposed in the housing 10 and above the detection seat 300, and is used for performing corresponding operations on the reagent kit 20, wherein the corresponding operations include a pipetting operation, an air bubble-pumping blending operation, a stirring blending operation, and a sealing membrane puncturing operation. When the mounting head needs to be unloaded (including both the cases of normal detection completion and accidental power failure), the pipette 400 moves to a preset position (for example, the front pool 120 in fig. 12) outside the mounting head accommodating pool to perform mounting head unloading operation, so that it is ensured that the pipette 400 does not repeatedly place the mounting head when the mounting head accommodating pool is already provided with the mounting head under the situation of accidental power failure restart, the running path for unloading the mounting head can be shortened, and meanwhile, the alignment requirement for unloading the mounting head is reduced (the caliber of the general mounting head accommodating pool is relatively small, and accurate alignment is required during unloading).

The present embodiment further provides a POCT blood cell analyzer, which includes a housing 10, a detection seat 300, a pipette 400, and a detector (which may be a detection element such as an optical coupler 405).

The test socket 300 can extend into or out of the housing 10 for receiving the reagent cartridge 20 provided with the impedance test cell, and the test socket 300 is provided with a power supply for matching impedance test. The reagent cartridge 20 has a mounting head receiving bay for mounting a mounting head.

The pipette 400 is disposed in the housing 10 above the test seat 300 for performing corresponding operations on the reagent cartridge 20.

The detector is used for detecting whether the mounting head is loaded on the liquid transfer device 400 when the POCT blood cell analyzer needs to unload the mounting head, if the liquid transfer device 400 is detected to be loaded with the mounting head, the liquid transfer device 400 moves to a mounting head accommodating pool or a preset position beyond the mounting head accommodating pool to execute mounting head unloading operation, and whether the mounting head is loaded on the liquid transfer device 400 or not can be known through the detector, so that unloading operation can be selectively carried out, and the POCT blood cell analyzer is relatively more intelligent.

The present embodiment also provides a POCT blood cell analyzer for receiving the package of the reagent kit 20, and the POCT blood cell analyzer includes a detection seat 300 and a pipette 400.

The detection seat 300 is used for receiving the reagent kit 20 provided with the impedance detection cell, and the detection seat 300 is provided with a power supply part matched with impedance detection. The reagent cartridge 20 has a mounting head receiving bay for mounting a mounting head.

The pipette 400 is disposed above the detection seat 300, and is configured to load the mounting head to perform corresponding operations on the reagent cartridge 20, and when the mounting head needs to be unloaded, the pipette 400 moves to the mounting head accommodating pool or a predetermined position outside the mounting head accommodating pool to perform mounting head unloading operations. In this embodiment, the POCT blood cell analyzer further includes a detector (which may be a detection element such as an optical coupler 405), where the detector is configured to detect whether the mounting head is loaded on the pipette 400 when the POCT blood cell analyzer needs to unload the mounting head, and if the mounting head is detected to be loaded on the pipette 400, the pipette 400 unloads the mounting head to the mounting head receiving pool or a preset position other than the mounting head receiving pool. The operation of unloading the mounting head to a preset position outside the mounting head accommodating pool by the liquid transfer device 400 is simpler, that is, the pool body with a large caliber is directly found nearby for unloading, so that the mounting head can be prevented from being repeatedly placed under the condition that the mounting head accommodating pool is already provided with the mounting head, the running path for unloading the mounting head can be shortened, and the alignment requirement for unloading the mounting head can be reduced.

Wherein, the kit 20 has a plurality of cell bodies, and the preset position is one of the cell bodies. The plurality of tank bodies include a mounting head accommodating tank matched with the mounting head and a plurality of function tanks, and the pipettor 400 unloads the mounting head into any one of the function tanks. The functional pools may include a diluent pool 111, a forebay 120 (WBC or RBC detection pool), etc., and the unloading of the mounting head by the pipettor 400 into any one of the functional pools is actually a selection of an absolute empty space and a relatively large open pool location.

As shown in fig. 6, the pipette 400 includes an airway tube 401 and an outer sleeve 402, and the mounting head is unloaded when the outer sleeve 402 moves axially relative to the airway tube 401.

The embodiment also provides a using method of the POCT blood cell analyzer, which comprises the following steps:

the liquid transfer device 400 of the POCT blood cell analyzer moves to the mounting head accommodating Chi Jiazai to carry out related operations of first detection;

the first detection has two conditions of normal detection completion and accidental power failure, before the second detection is carried out, the mounting head used in the first detection needs to be unloaded, and the pipettor 400 of the POCT blood cell analyzer moves to a preset position outside the mounting head accommodating pool to execute mounting head unloading operation. The use method can ensure that the liquid shifter 400 can not repeatedly place the mounting head under the condition that the mounting head accommodating pool is provided with the mounting head, can also shorten the running path when the mounting head is unloaded, and simultaneously reduces the alignment requirement when the mounting head is unloaded.

the first detection has two conditions of normal detection completion and accidental power failure, before the second detection, the mounting head used in the first detection needs to be unloaded, and the detector can detect whether the mounting head is loaded on the liquid transfer device 400 of the POCT blood cell analyzer;

if it is detected that the mounting head is loaded on the pipette 400, the pipette 400 moves to a predetermined position outside the mounting head accommodating pool to perform mounting head unloading operation.

The use method can know whether the mounting head is loaded on the liquid moving device 400 or not and further selectively carry out unloading operation, and is relatively more intelligent.

The present embodiment also provides a method for using a POCT blood cell analyzer, including:

moving a liquid transfer device of the POCT blood cell analyzer to the mounting head accommodating Chi Jiazai for carrying out related operations of first detection;

the first detection has two conditions of normal detection completion and accidental power failure, and before the second detection is carried out, the reagent kit 20 and the mounting head used in the first detection need to be taken out, so whether the POCT blood cell analyzer is provided with the reagent kit 20 needs to be detected;

before the second detection, whether a mounting head is loaded on the pipette 400 of the POCT hemocytometer may be detected by a detector;

if the fact that the POCT hematology analyzer is equipped with the reagent kit 20 and the mounting head is loaded on the pipette 400 is detected, the pipette 400 is controlled to move to the mounting head accommodating pool or a preset position outside the mounting head accommodating pool to execute mounting head unloading operation. When the preset position outside the mounting head accommodating pool is selected to execute the mounting head unloading operation, the mounting head cannot be repeatedly placed under the condition that the mounting head accommodating pool is already provided with the mounting head, the running path of the mounting head during unloading can be shortened, and meanwhile, the alignment requirement of the mounting head during unloading is reduced.

The preset position may correspond to the inside of the test socket 300 or to the outside of the test socket 300. That is, the unloading operation is not limited to unloading the mounting head onto the test socket 300 or onto the reagent cartridge 20, but may be unloading directly to an area other than the test socket 300 inside the instrument, for example, a mounting head recovery cassette is provided inside the instrument, and the maintenance person periodically empties the mounting head recovery cassette.

The mounting head may be unloaded by axial movement of the outer sleeve 402 of the pipette 400 relative to the airway 401 of the pipette 400. In a specific embodiment, the pipette 400 unloads the mounting head through a fixed stop or a following retractable stop 4031, the stop 4031 is a retractable motor, a vertically or horizontally disposed electromagnet, or a U-shaped orifice, wherein the outer sleeve 402 may be connected with the synchronizing block 403, and when the stop 4031 stops the synchronizing block 403 and the pipette 400 is raised, the mounting head is unloaded by the lower end of the outer sleeve 402.

Referring to fig. 1 to 13 together, the present embodiment provides a pipette 400, where the pipette 400 includes a gas tube 401 and a detecting element.

The airway tube 401 is used for loading an installation head (tip head 201 or puncture head 204), and the detection piece is arranged at the side edge of the airway tube 401 and used for detecting that the installation head is loaded and connected on the airway tube 401.

The pipette 400 further comprises an outer sleeve 402, the outer sleeve 402 is sleeved on the periphery of the air duct 401, when the air duct 401 is loaded with the mounting head, the air duct 401 is inserted into the mounting head, so that the mounting head pushes the outer sleeve 402 to generate position change along the axial direction of the air duct 401, and the detection piece is used for detecting the position of the outer sleeve 402 so as to judge whether the mounting head is sleeved on the air duct 401.

When the airway tube 401 is sleeved with the mounting head, the outer sleeve 402 is located at a first position (a state position shown in fig. 6) of the airway tube 401, and when the mounting head is not sleeved on the airway tube 401, the outer sleeve 402 is located at a second position of the airway tube 401, and the first position is higher than the second position.

The outer sleeve 402 is connected with a light blocking sheet 404, the detection piece is an optical coupler 405 matched with the light blocking sheet 404, when the optical channel of the optical coupler 405 is blocked by the light blocking sheet 404, the air duct 401 is connected with the mounting head in a sleeved mode, and when the optical channel of the optical coupler 405 is not blocked by the light blocking sheet 404, the air duct 401 is connected with the mounting head in a non-sleeved mode.

The pipette 400 includes a fixing plate 406 disposed at a side of the air duct 401, and the fixing plate 406 is optically coupled to the pipette. The air duct 401 of the pipette 400 can independently move up and down, and the entire pipette 400 can move in two or three dimensions.

The fixing plate 406 is provided with a through slot 407, a pair of light receiving and emitting portions of the optocoupler 405 extend into the through hole and are located on the side of the airway tube 401, the outer cannula 402 is further connected with a synchronizing block 403, the synchronizing block 403 extends into the through slot 407, the airway tube 401 rises and the mounting head is unloaded when the synchronizing block 403 is blocked, after the mounting head is unloaded, the outer cannula 402 is still sleeved on the periphery of the airway tube 401, and because the synchronizing block 403 extends into the through slot 407, the outer cannula 402 does not fall off relative to the airway tube 401, but is naturally supported at the bottom of the through slot 407 (i.e., at the second position).

before pipetting, judging whether a mounting head is arranged on an air duct 401 of the detection pipette 400;

if the fact that the mounting head is not mounted on the air duct 401 of the pipette 400 is detected, an alarm is given. Since the pipeline of the POCT blood cell analyzer is air flow, not liquid flow, and the pipeline cannot suck liquid, the air duct 401 must be operated when the mounting head (tip head 201) is sleeved, and therefore, it is necessary to detect whether the mounting head is mounted on the air duct 401 of the pipette 400.

In the step of detecting whether the mounting head is mounted on the air duct 401 of the pipette 400, the outer sleeve 402 is sleeved on the periphery of the air duct 401, and the position of the outer sleeve 402 is detected by the detection part so as to judge whether the mounting head is sleeved on the air duct 401.

When the airway tube 401 is sleeved with the mounting head, the outer sleeve 402 is located at a first position of the airway tube 401, and when the mounting head is not sleeved on the airway tube 401, the outer sleeve 402 is located at a second position of the airway tube 401, wherein the first position is higher than the second position.

The detection piece is an optical coupler 405, and a light blocking sheet 404 matched with the optical coupler 405 is correspondingly arranged on the outer sleeve 402. When the light blocking sheet 404 blocks the light channel of the optical coupler 405, it is determined that the mounting head outer sleeve 402 is sleeved on the air duct 401.

The present embodiment provides a POCT blood cell analyzer, which includes a housing 10, a detection seat 300, a pipette 400, and a detection member.

The detection seat 300 can extend into or out of the shell 10 and is used for receiving the reagent box 20 provided with the impedance detection cell; the pipette 400 is arranged in the shell 10 and positioned above the detection seat 300, the pipette 400 comprises a gas guide tube 401, and the gas guide tube 401 is used for loading a mounting head (tip head 201 or puncture head 204); the detection piece is arranged on the side edge of the air duct 401 and used for detecting whether the installation head is loaded on the air duct 401, and the detection piece can be an optical coupler 405 and other detection elements capable of detecting whether an object exists.

In this embodiment, the loading state of the mounting head can be known by the detection element, so that the phenomenon that the internal gas circuit of the POCT blood cell analyzer is polluted by the liquid transfer operation of the liquid transfer device in the state that the mounting head is not loaded is avoided.

In a third embodiment, referring to fig. 1 to 13 together, the present embodiment provides a POCT blood cell analyzer, which includes a detection seat 300, a liquid transfer device 400 disposed above the detection seat 300, and a pressure build-up system, wherein the detection seat 300 is used for receiving the reagent kit 20, the reagent kit 20 is provided with a front cell 120 and a rear cell communicated through a micro-hole, and the pressure build-up system includes a pressure chamber 210, an air pressure connection device 510, a first syringe 222, a second syringe 223, and a driving member 224.

The first injector 222 is communicated with the pressure chamber 210 or the pipette 400, and is used for establishing positive pressure or negative pressure in the pressure chamber 210, and is also used for assisting the pipette 400 to perform pipetting, or is used for bubbling and mixing liquid in the forebay 120, the second injector 223 is communicated with the pipette 400, and is used for sucking and spitting samples and reagents, the driving member 224 is used for simultaneously driving the first injector 222 and the second injector 223, the first injector 222 and the second injector 223 may be linked injectors, so that the same driving member 224 is conveniently shared, the driving member 224 may be a motor, and of course, the first injector 222 and the second injector 223 may also be split injectors and independently controlled by different motors.

The POCT blood cell analyzer also comprises electromagnetic valves, wherein the electromagnetic valves comprise a first electromagnetic valve SV1, a second electromagnetic valve SV2 and a third electromagnetic valve SV3.

A first solenoid valve SV1 selectively communicates the first syringe 222 with the pressure chamber 210 and the second syringe 223; the second solenoid valve SV2 selectively communicates the first solenoid valve SV1 with the pressure chamber 210, the outside atmosphere, which is connected to the second solenoid valve SV2 through the air filter 225, the pipe T10; the third solenoid valve SV3 selectively communicates the pneumatic connection 510 with the pressure chamber 210, and the lines T8 and T7 communicate via the third solenoid valve SV3 when the third solenoid valve SV3 is energized.

The first injector 222 may draw air through the air filter 225, the T10 line, the second solenoid valve SV2, T5 line, and the first solenoid valve SV1, T1 line, and then establish a positive pressure to the pressure chamber 210 through the T1 line, the first solenoid valve SV1, T5 line, and the second solenoid valve SV2, T6 line.

The first injector 222 can suck the gas in the pressure chamber 210 through the T1 line, the first solenoid valves SV1, T5 line, and the second solenoid valves SV2, T6 line to establish a negative pressure, and then exhaust the gas through the T1 line, the first solenoid valves SV1, T5 line, the second solenoid valves SV2, T10 line, and the air filter 225.

After the pressure chamber 210 establishes the positive pressure or the negative pressure, the positive pressure or the negative pressure can be output through the T7 pipeline, the third solenoid valve SV3, the T8 pipeline and the pneumatic connecting device 510. The T8 pipeline may be a relatively hard pipeline, that is, the hardness of the T8 pipeline is greater than that of the second conduit 221, and the greater hardness of the T8 pipeline can prevent the pipe wall of the T8 pipeline from being attached together to block the internal airflow channel of the T8 pipeline when outputting negative pressure.

When the first solenoid valve SV1 connects the T1 pipeline with the T2 pipeline, the positive pressure or negative pressure generated by the first syringe 222 and the second syringe 223 acts on the pipette 400 at the same time, that is, when a large amount of sample suction or sample push is required, the first syringe 222 can play a role in assisting in pipetting.

The volume of the first syringe 222 is larger than that of the second syringe 223, the first syringe 222 is mainly used for pressure buildup, the second syringe 223 is mainly used for sample pushing/sucking, and the two syringes can be used for pressure buildup or sample pushing/sucking as required.

The ratio of the volume of the first syringe 222 to the volume of the second syringe 223 may be 80 to 120:1, for example, the first syringe 222 has a volume of 10 ml and the second syringe 223 has a volume of 100. Mu.l. The driver 224 drives the piston rods of the first syringe 222 and the second syringe 223 through the linkage plate 231 so that the first syringe 222 and the second syringe 223 move synchronously. Wherein, the piston rod of the second syringe 223 is in sealing fit with the tail end of the outer cylinder of the second syringe 223.

The POCT blood cell analyzer further includes a housing 10 and a rack 301, the pressure chamber 210 is disposed on the housing 10 or on the rack 301, and the pipette 400, the first syringe 222, the second syringe 223, and the driving member are disposed on the rack 301.

The detection seat 300 is slidably or rotatably disposed with respect to the frame 301 and located below the pipette 400.

The POCT blood cell analyzer further includes a metal shielding cover 500, the detecting base 300 is capable of moving to a liquid preparation station (i.e. the position shown in fig. 3) relative to the frame 301 so as to facilitate corresponding operations of the liquid transfer device 400, the detecting base 300 is capable of moving to a sample detection station (not shown in the figure, corresponding to the position right below the metal shielding cover 500) relative to the frame 301, and the metal shielding cover 500 is configured to descend at the sample detection station to cooperate with the detecting base 300 to form a relatively closed metal cavity to shield external electromagnetic interference. The rack 301 may have a double-layered structure, in which the lower layer is provided with the detection seat 300, and the upper layer is provided with the pipette 400, the metal shielding cap 500, and the syringe 220.

As shown in fig. 1, the housing 10 is provided with a display 11 and a delivery port 12, the testing seat 300 is used for moving to the delivery port 12 to receive the reagent kit 20 and loading the reagent kit into the testing seat 300, and the distance between the liquid preparation station and the delivery port 12 is smaller than the distance between the sample detection station and the delivery port 12, that is, the delivery port 12 is the liquid preparation station and the sample detection station in sequence towards the inside of the instrument. The detection base 300 reciprocates between the loading station, the liquid preparation station and the sample detection station, and the liquid preparation station is positioned between the loading station and the sample detection station. In other implementations, the liquid dispensing station and the sample testing station may be the same station.

The present embodiment further provides a method for using the POCT blood cell analyzer, which includes the steps of:

controlling the movement of the driver 224 to establish positive or negative pressure to the pressure chamber 210 via the first syringe 222;

controlling the drive 224 to move to pipette through the second syringe 223 and the pipette 400;

the pressure release of the pressure chamber 210 is controlled to apply positive pressure to the front cell 120 or negative pressure to the back cell via the pneumatic connection 510 to achieve the flow of liquid in the front cell 120 to the back cell through the micropores.

The POCT blood cell analyzer that this embodiment provided is rationally distributed, compact structure, convenient to use.

Referring to fig. 1 to 13, the present embodiment provides a kit 20, wherein the kit 20 includes a box 100 and a microfluidic test strip 600.

The cartridge 100 includes an impedance detection cell (including a front cell 120, a rear cell, and a pressure chamber 140, which are connected to each other) for performing an impedance detection.

The microfluidic test strip 600 is integrally connected, assembled or not connected to the cartridge 100 and is located at the side of the impedance test cell.

The microfluidic test strip 600 is provided with a sample application hole 601 for receiving a sample to be laid in the microfluidic test strip 600 after entering.

The cartridge 100 further comprises a diluent tank 111, a hemolytic agent tank 106, an optical detection tank, and a staining lysis tank 602, wherein the diluent tank 111 is used for packaging diluent, the hemolytic agent tank 106 is used for packaging hemolytic agent, the staining lysis tank 602 is used for packaging staining lysis agent, and the optical detection tank is integrally connected or assembled with the cartridge 100.

The cartridge 100 further comprises a mounting head receiving chamber for receiving a mounting head (tip head 201 or) puncture head 204, and a sample receiving chamber for receiving a sample tube.

Referring to fig. 13, the present embodiment further provides a POCT blood cell analyzer, which includes a detecting base 300, an image recognition base 610, and an image recognition device 620.

The detection seat 300 is provided with a power supply (such as the conductive holder 303 shown in fig. 9) for matching impedance detection, the detection seat 300 is used for receiving the loading of the reagent kit 20, the image recognition base 610 is arranged at the side of the detection seat 300, and the image recognition device 620 is arranged above the image recognition base 610 and is used for matching with the image recognition base 610 to perform image detection on the microfluidic detection chip 600.

The POCT hematology analyzer further comprises a liquid transfer device 400, the liquid transfer device 400 is arranged above the detection seat 300, the POCT hematology analyzer comprises a metal shielding cover 500 matched with the detection seat 300, the metal shielding cover 500 can move up and down or rotate, and the image recognition device 620 is independently arranged or connected with the metal shielding cover 500.

The POCT blood cell analyzer and the kit 20 provided by the embodiment can simultaneously support impedance detection and microfluidic image detection, and have optimized joint detection efficiency and better effect.

In a fifth embodiment, referring to fig. 1 to 13, the present embodiment provides a POCT blood cell analyzer, which includes a frame 301, a detection seat 300, and a pipette 400.

The testing seat 300 is slidably or rotatably disposed relative to the rack 301, the testing seat 300 reciprocates between a loading station and a sample testing station, and the testing seat 300 slides out of or is screwed out of the rack 301 to be located at the loading station to receive the reagent cartridge 20 to load the reagent cartridge 20 or allow the reagent cartridge 20 to be taken out.

The POCT blood cell analyzer also comprises a liquid preparation station; the liquid preparation station is arranged between the loading station and the sample detection station; or the liquid preparation station and the sample detection station are the same station.

The testing socket 300 is a semi-surrounding shape with an opening at the upper part, the testing socket 300 is matched with the reagent kit 20 in a snap fit mode, and the testing socket 300 comprises a body part 310 and metal shielding sockets (320, 321 and 322) wrapping the periphery of the body part 310.

The POCT cytometer also includes a metal shield cover 500 that mates with the metal shield mounts (320, 321, 322). The metal shielding cover 500 is disposed above the test socket 300 and is configured to be movable in a vertical direction at the sample testing station, and a vertical edge of the metal shielding cover 500 abuts against an upper surface of the metal shielding socket (320).

The POCT blood cell analyzer further includes a pressure building system, one end of the pressure building system is provided with an air pressure connecting device 510 extending into the metal shielding cover 500 and moving synchronously with the metal shielding cover 500, and the reagent kit 20 is provided with a pressure acting cavity 140 in butt joint with the air pressure connecting device 510.

The embodiment provides a using method of a POCT blood cell analyzer, which comprises the following steps:

the test seat 300 slides out or rotates out of the rack 301 to receive the reagent cartridge 20 at the loading station;

the detection seat 300 slides into or is screwed into the frame 301 and moves to a liquid preparation station;

the pipettor 400 moves to load a mounting head (tip head 201 or puncture head 204) pre-placed on the kit 20 to perform corresponding operations so as to complete liquid preparation;

the detection seat 300 moves from the liquid preparation station to the sample detection station below the metal shielding cover 500;

the metal shielding cover 500 moves downward to be engaged with the detection base 300;

the pneumatic connection 510 connected to the metal shielding cover 500 starts to provide pressure to drain the liquid in the impedance measuring cell on the reagent cartridge 20 for impedance measurement.

The POCT blood cell analyzer and the using method thereof provided by the embodiment have high automation degree, and pretreatment liquid preparation operation of the liquid to be detected is automatically completed in the analyzer, so that the randomness and the error of manual operation are avoided.

In a sixth embodiment, referring to fig. 1 to 13, the present embodiment provides a POCT blood cell analyzer, which includes a detection seat 300 and a metal shielding cover 500.

The detection base 300 reciprocates between the loading station and the sample detection station, and is used for receiving the reagent kit 20 with the impedance detection cell at the loading station, and the metal shielding cover 500 is arranged at the sample detection station and is used for covering the detection base 300 when the detection base 300 moves to the sample detection station so as to shield the electromagnetic signal. The POCT blood cell analyzer also comprises a liquid preparation station; the liquid preparation station is positioned between the loading station and the sample detection station; or the liquid preparation station and the sample detection station are the same station.

Detect seat 300 and be the upper portion open-ended and enclose the form partly, metal shield cover 500 is the open-ended and enclose the form partly of lower part, detects seat 300 and kit 20 snap-fit, detects seat 300 and includes this body portion 310 and wraps in the metal shield seat (320, 321, 322) of this body portion 310 periphery, detects seat 300 and sets up to be able to be in metal shield cover 500 below translation or rotation, and metal shield cover 500 sets up to be able to move from top to bottom in vertical direction.

the test seat 300 slides out or unscrews out of the rack 301 to receive the loading of the reagent cartridge 20 at the loading station;

the liquid transfer device 400 performs corresponding operations on the reagent kit 20 at the liquid preparation station;

the metal shielding cover 500 is covered with the detection base 300 and the detection is performed by an impedance method, specifically, the detection base 300 moves to the lower side of the metal shielding cover 500, and then the metal shielding cover 500 moves downwards to cover the detection base 300.

Wherein, the liquid preparation of the kit 20 by the pipette 400 comprises: the pipette 400 sucks a blood sample and a reagent, including a hemolytic agent, a diluent, and the like, pre-loaded in the reagent cartridge 20 and moves into a detection cell of the reagent cartridge 20.

The POCT blood cell analyzer and the using method thereof provided by the embodiment have high automation degree, and the pretreatment liquid preparation operation of the liquid to be detected is automatically completed in the analyzer, so that the randomness and the error of manual operation are avoided.

In a seventh embodiment, referring to fig. 1 to 13, the present embodiment provides a testing socket 300, wherein the testing socket 300 includes a testing cavity 302 and a conductive holder 303.

The detection cavity 302 is used for receiving and loading a reagent box 20 provided with an impedance detection cell, the reagent box 20 is provided with electrodes, and the conductive support 303 is used for electrically connecting the electrodes. The electrodes are conductive posts 122 disposed on opposite sides of the cartridge 20.

As shown in fig. 10, in an embodiment, the conductive holder 303 includes a downwardly inclined spring portion 304, and the spring portion 304 is used for holding and electrically connecting the conductive pillar 122. The conductive holder 303 further includes a fixing connection portion 305 integrally connected to the elastic piece portion 304, and the shape of the fixing connection portion 305 is not limited, and a fixing hole may be provided to facilitate fastening assembly by a screw.

As shown in fig. 11, in another embodiment, the conductive holder 303 includes a fixed connection portion 305 and a bending portion 306 (which may be a V-shaped bending portion or an arc-shaped bending portion) extending and bending, the bending portion 306 is pushed open when the conductive pillar 122 is assembled from top to bottom, and the bending portion 306 resets and abuts against the conductive pillar 122 when the conductive pillar 122 is assembled in place. The conductive base 303 may be one and disposed on one side of the conductive pillar 122, or two and disposed on two sides of the conductive pillar 122 respectively to further increase the reliability of the electrical connection. Specifically, the bending portion 306 may include a first bending portion 3061 and a second bending portion 3062 connected to the fixed connection portion 305 in sequence, the first bending portion 3061 is pushed open when the conductive pillar 122 is assembled from top to bottom, and the second bending portion 3062 abuts against the conductive pillar 122 when the conductive pillar 122 is assembled in place.

The detection seat 300 further comprises a body part 310 and metal shielding seats (320, 321, 322), wherein the body part 310 is provided with a detection cavity 302, the side wall of the body part 310 is provided with an assembling groove 307 communicated with the detection cavity 302, the conductive support 303 extends into the detection cavity 302 through the assembling groove 307, the metal shielding seats (320, 321, 322) are covered on the periphery of the body part 310 and are insulated and spaced from the conductive support 303, and the metal shielding seats (320, 321, 322) comprise side plates (320, 321) surrounding the body part 310 and a bottom plate (322) attached to the bottom of the body part 310.

The kit 20 further comprises an integrally connected or detachable optical detection cell, the detachable optical detection cell can be inserted through the rectangular insertion hole 107, the detection seat 300 further comprises an optical detection assembly 330 corresponding to the optical detection cell, the optical detection assembly 330 comprises a light emitting assembly, a light receiving assembly and a detection cavity located between the light emitting assembly and the light receiving assembly, the included angle range between the axis of the light emitting assembly and the axis of the light receiving assembly is 0-60 degrees, and specifically can be 0 degree, 20 degrees, 30 degrees, 45 degrees, 60 degrees and the like.

The optical detection elements 330 are in multiple groups, and the light source cavities of the multiple groups of optical detection elements 330 are separated from each other by the light blocking wall 333 to avoid the mutual interference of the detection lights.

For example, the multiple sets of optical detection components 330 may include two sets of laser detection components 331 and one set of LED light source detection components 332, a light blocking wall 333 is disposed between the detection cavities of the laser detection components 331, and the light blocking wall 333 is preferably blackened, so that the light absorption effect and the light reflection effect are better, and the influence of the reflected light on the normal optical detection is avoided.

The detection base 300 further comprises a peltier 340, a heat sink 350 and a fan 360 which are sequentially arranged close to the detection cavity of the laser detection component 331, the heat sink 350 comprises a heat absorption substrate 351 attached to the peltier 340 and heat dissipation fins 352 vertically connected with the heat absorption substrate 351, the fan 360 can be arranged at one end, away from the heat absorption substrate 351, of the heat dissipation fins 352, the detection temperature can be controlled by arranging the peltier 340, the heat sink 350 and the fan 360, and the detection result obtained by detection at the preset detection temperature is relatively more accurate.

The bottom of the detection cavity 302 is provided with a tact switch 334, the tact switch 334 can know the loading condition of the reagent kit 20, when the reagent kit 20 is loaded, the tact switch 334 is pressed, and when the reagent kit 20 is not loaded, the tact switch 334 is in a bounce state.

The present embodiment further provides a POCT hematology analyzer, which includes a pipette 400, the aforementioned detection seat 300, and the pipette 400 disposed above the detection seat 300, wherein the pipette 400 is used for performing corresponding operations on the reagent kit 20.

The conductive holder 303 of the POCT blood cell analyzer and the detection seat 300 thereof provided by the embodiment includes the elastic sheet part 304 inclined downwards, so that the POCT blood cell analyzer has better adaptability and stability when being electrically connected and matched with the electrode of the reagent kit 20, and the possibility of poor contact is greatly reduced.

In an eighth embodiment, the present embodiment provides a method for using a POCT blood cell analyzer, comprising the steps of:

in response to the reagent cartridge 20 being placed into the detection seat 300, controlling the pipette 400 to move to above the first mounting head accommodation well of the reagent cartridge 20 and descend to load the first mounting head, wherein the first mounting head may be the tip head 201;

controlling the pipette 400 to move to the diluent reservoir 111 of the kit 20 to move the diluent into the forewell 120 (i.e., WBC and/or RBC detection reservoir) for performing the impedance method detection;

controlling the pipettor 400 to move to the sample tube/sample dilution well/other sample placement location to move the sample into the forewell 120, wherein the sample in the sample dilution well is the diluted sample;

inflating, bubbling and uniformly mixing or stirring the liquid to be detected in the forebay 120;

controlling the metal shielding cover 500 to cover the detection seat 300 to establish a communication relationship between the rear pool of the reagent kit 20 and the pressure building system;

controlling the pressure building system to guide the liquid to be tested from the front pool 120 into the rear pool;

and detecting the liquid to be detected by an impedance detection method.

Wherein controlling the pipette 400 to move above the first mounting head receiving bay of the reagent cartridge 20 and to descend to load the first mounting head comprises: the pipetter 400 is controlled to move three-dimensionally with respect to the first mounting head placement tank, so that the pipetter 400 and the first mounting head are pressed against each other to be loaded onto the first mounting head.

Controlling the pipettor 400 to move to the diluent well 111 of the reagent cartridge 20 to move the diluent well into the forewell 120 for impedance testing and/or controlling the pipettor 400 to move the sample into the forewell 120 where the sample tube/sample diluent well/other sample is placed includes: the pipette 400 moves in three dimensions relative to the forebay 120 and uses the first mounting head cavity, typically having a volume of 100 microliters to 1000 microliters, as a transfer location for the liquid to draw in the diluent and/or the sample, and typically does not exceed 4/5 of this volume when in use to avoid contamination of the liquid into the tubing of the pressurized system.

Uniformly mixing the liquid to be detected in the forebay 120, wherein the liquid to be detected is sucked and spitted by the liquid transfer device 400 through the first mounting head; or the liquid transfer device 400 injects bubbles into the liquid to be measured through the first mounting head; or the liquid transfer device 400 stirs the liquid to be detected through the first mounting head.

After the liquid to be tested in the forebay 120 is uniformly mixed, the liquid transfer device 400 is further included to unload the first mounting head to the first mounting head accommodating bay.

The pipette 400 unloading the first mounting head to the first mounting head receiving bay further includes the pipette 400 moving above the second mounting head receiving bay and lowering to load the second mounting head.

The pipetter 400 includes after moving to the second mounting head accommodation pool and descending to load the second mounting head:

the pipettor 400 moves to the sample tube/sample dilution cell/other sample placement to move the sample into the light detection cell;

and detecting the liquid to be detected by an optical detection method.

The pipette 400 includes, after moving to the second mounting head accommodation pool and descending to load the second mounting head:

the pipettor 400 moves to a sample tube to move the sample into the staining lysis cell 602 for incubation and then moves into the microfluidic detection sheet 600;

the particles in the microfluidic test strip 600 are detected by image detection.

The present embodiment further provides a POCT blood cell analyzer, which includes a detection seat 300, a pipette 400, a pressure build system, a metal shielding cover 500, and a transmission assembly.

The detection seat 300 is used for receiving the reagent kit 20, the reagent kit 20 is provided with a front pool 120 and a rear pool which are communicated through a micropore, and a first mounting head, diluent and a sample are arranged on the reagent kit 20;

the pipettor 400 is used to load the first mounting head and move the diluent, sample into the forebay 120;

the metal shielding cover 500 is used for covering the detection seat 300, and the pressure building system is used for leading the liquid to be detected from the front pool 120 into the rear pool.

The transmission assembly is used for driving the detection seat 300, the pipettor 400 and the metal shielding cover 500 to perform one-dimensional, two-dimensional or three-dimensional motion, and the transmission assembly may be formed by a motor, a screw rod, a nut, a slide rail, a slide block, a gear, a rack, a synchronous belt, and the like.

The reagent cartridge 20 is further provided with a second mounting head and an optical detection pool, and the pipette 400 is further used for loading the second mounting head and moving the sample into the optical detection pool for optical detection.

Or, the kit 20 is further provided with a second mounting head, a staining lysis reagent, a microfluidic detection sheet 600, and the poct hematology analyzer further comprises an image detection base 610 and an image detection device 620 which are arranged at intervals, the pipette 400 is further used for loading the second mounting head and transferring the sample into the staining lysis reagent for incubation, then transferring the incubated sample into the microfluidic detection sheet 600, and performing image detection on the microfluidic detection sheet 600 through the image detection base 610 and the image detection device 620.

The POCT blood cell analyzer and the using method thereof provided by the embodiment have the advantages that the automation degree is high, the automatic liquid distribution is completed in the analyzer, the consistency of the process operation is good, and the impedance detection, the optical detection and the image detection can be simultaneously supported.

In a ninth embodiment, referring to fig. 1 to 13, the present embodiment provides a POCT blood cell analyzer, which includes a detecting seat 300, a puncture head loading mechanism, a puncture head 204, and a pipette 400. Wherein the puncture head loading mechanism may be an independent mechanism or the same mechanism as the pipette 400.

The test seat 300 is used for containing the reagent kit 20, and the reagent kit 20 is provided with a diluent pool and a hemolytic agent pool, and the openings of the diluent pool and the hemolytic agent pool are provided with sealing films.

The puncture head 204 is used for puncturing at least two sealing films on the kit 20 loaded in the POCT blood cell analyzer one by one, and the puncture head loading mechanism is used for loading the puncture head 204.

The piercing tip 204 is provided on the cartridge 20 and/or on the test seat 300. The kit 20 and/or the detection seat 300 are provided with placement positions matched with the puncture head 204, and the puncture head 204 is loaded by the liquid transfer device 400 and then synchronously moves along with the liquid transfer device 400.

The puncture head 204 further horizontally swings after passing through the sealing film, whereby the diameter of the through hole formed in the sealing film can be enlarged.

Preferably, at least two sealing films are located on the same straight line or on the same arc, so that the movement path of puncturing one by one is relatively simple.

As shown in fig. 7, puncture head 204 includes a main body 2042 and a pointed portion 2041, and pointed portion 2041 is provided at one end of main body 2042. The cross section of the sharp portion 2041 may be in the shape of a straight line, a cross, a Chinese character mi, a Y, or the like. The main body 2042 is provided with a flange 2044 at an end away from the sharp portion 2041, and the flange 2044 can facilitate the puncture head 204 to be hung on the puncture head accommodating pool 104, and can form a stable abutting fit with the outer sleeve 402.

Referring to fig. 1 to 13 together, the present embodiment provides a POCT blood cell analyzer, which includes a testing seat 300 and a mounting head.

The test seat 300 is used for loading the reagent box 20, the reagent box 20 is provided with a diluent pool and a hemolytic agent pool, openings of the diluent pool and the hemolytic agent pool are provided with sealing films, and the mounting head is used for puncturing the sealing films on the reagent box 20 loaded in the POCT hematology analyzer one by one. In this embodiment, the mounting head is a tip head 201 or a dedicated piercing head 204, and is provided on the cartridge 20. The kit 20 is provided with a placing position matched with the mounting head, the outer diameter of the puncture head 204 is larger than that of the tip head 201, and the opening formed after the puncture head 204 penetrates through the sealing film is larger than the diameter of the opening, into which the tip head 201 extends, so that a negative pressure cavity can be prevented from being formed in the tank when the tip head absorbs liquid, and the liquid absorption accuracy is ensured.

The POCT blood cell analyzer further includes a pipette 400, and the mounting head moves with the pipette 400 after being loaded by the pipette 400.

At least two sealing films are positioned on the same straight line or the same arc line. The movement path of the pricking operation one by one can be relatively simple. The mounting head further swings horizontally after penetrating the sealing film to enlarge the caliber of the through hole on the sealing film.

In an embodiment, the tip head 201 can prick the first through hole with the first stroke first, prick the second through hole with the second stroke again in order to inhale kind, and the first stroke is less than the second stroke, forms the precision when negative pressure sealed chamber influences imbibition from this when can avoiding imbibition.

In another embodiment, the tip head is lifted after the through hole is pierced and then sample sucking is carried out, and the phenomenon that the accuracy of liquid sucking is affected by forming a negative pressure sealing cavity during liquid sucking can be avoided.

The puncture head 204 includes a main body 2042 and a pointed portion 2041 provided at an end of the main body 2042.

The sharp portion 2041 has a cross-section in the shape of a straight line, a cross, a Chinese character mi, a Y, or the like. The main body 2042 is provided with a flange 2044 at an end thereof remote from the sharp part 2041.

In this embodiment, the tip head 201 may be used as it is, or a dedicated piercing head 204 may be used for piercing.

Referring to fig. 1 to 13 together, the eleventh embodiment provides an analyzer for POCT blood cells, which includes a housing 10, a pipette 400, and a piercing tip 204.

The pipettor 400 is arranged in the casing 10, the pipettor 400 includes an air duct 401, the puncture head 204 includes a main body portion 2042 and a sharp portion 2041, the sharp portion 2041 is arranged at one end of the main body portion 2042, and the other end of the main body portion 2042 is provided with an accommodating cavity 2045 for the air duct 401 to insert.

The sharp portion 2041 is used to pierce a sealing film on the reagent cartridge 20 when the POCT blood cell analyzer performs automated detection. The cross section of the sharp portion 2041 is in a shape of a straight line, a cross, a meter, or a Y, and a flange portion 2044 is provided at an end of the main body portion 2042 away from the sharp portion 2041 to facilitate hanging on the case 100 of the reagent cartridge 20. POCT blood cell analyzer still includes detects the seat 300, detects the seat 300 and is used for installing kit 20, and kit 20 is equipped with the puncture head and places the pond, and flange portion 2044 bearing places the pond periphery in the puncture head.

The pipettor further comprises an outer sleeve 402 sleeved on the periphery of the air guide tube 401, the lower end of the outer sleeve 402 is abutted to the flange portion 2042, and one end, close to the flange portion 2044, of the main body portion 2042 is provided with a plurality of ribs 2043 extending towards the sharp portion 2041 so as to improve strength. Piercing head 204 may be integrally formed from a metal or plastic material.

As shown in fig. 12, this embodiment provides a kit 20, wherein the kit 20 comprises a plurality of well sites, and one of the well sites is used for placing the aforementioned puncture head 204.

The plurality of wells include a diluent well 111 and/or a hemolytic agent well 106, and the diluent well 111 and/or the hemolytic agent well 106 are provided with sealing films (not shown).

The cell site also includes a WBC detection cell and/or an RBC detection cell for performing impedance-based detection.

As shown in fig. 12, the kit 20 further includes a microfluidic test strip 600, and the microfluidic test strip 600 can perform image detection through an image detection base 610 and an image detection device 620.

In this embodiment, the puncturing operation of the sealing membrane does not need to be performed manually, and a better consistent operation can be realized by using a special puncturing head 204 or tip head 201.

In a twelfth embodiment, referring to fig. 1 to 13, the present embodiment provides a POCT blood cell analyzer, which includes a detecting base 300 and a pressure-building element.

The detection seat 300 is used for installing the kit 20, the kit 20 comprises an impedance detection pool for performing impedance detection, the impedance detection pool comprises a front pool 120 and a rear pool which are communicated through micropores, a pressure building component is communicated with the front pool 120 and/or the rear pool air passage and used for providing pressure so that liquid to be detected in the front pool 120 flows to the rear pool through the micropores, the pressure building component is also used for providing pressure to the front pool 120 or the rear pool before the liquid to be detected is injected into the front pool 120 so that the micropores are unblocked, when the pressure building component provides negative pressure, the impedance detection can be performed, and when the pressure building component provides positive pressure, the micropores can be backflushed so as to ensure the unblocking of the micropores.

The pressure build-up assembly includes an injector 220 and a pressure chamber 210 in air communication, the injector 220 being used to build up positive or negative pressure to the pressure chamber 210. The pressure building component is used for providing air flow to uniformly mix bubbles in the liquid to be measured or enabling the micropores to be unblocked, and specifically, the air blowing or the air suction can be carried out through the front pool 120/the rear pool to enable the micropores to be unblocked.

The POCT blood cell analyzer includes a plurality of sets of gate valves, which connect the pressure chamber 210 and the injector 220.

The POCT blood cell analyzer includes a metal shielding cover 500 engaged with the detection seat 300, the metal shielding cover 500 is provided with an air pressure connection device 510 communicated with the pressure chamber 210 or the syringe 220, and the air pressure connection device 510 is used for communicating with the rear cell or the front cell 120. The metal shield cover 500 is provided to move in a vertical direction.

The POCT blood cell analyzer further includes a pipette 400, and the pipette 400 is disposed above the detection seat 300 and is used for performing corresponding operations on the reagent kit 20.

The liquid transfer device 400 is communicated with the pressure building component through a pipeline, and the liquid transfer device 400 is used for loading a mounting head on the reagent kit 20 and performing corresponding operation through the mounting head.

the receiving reagent kit is loaded into the detection seat 300, the reagent kit comprises an impedance detection cell for performing impedance detection, and the impedance detection cell comprises a front cell 120 and a rear cell which are communicated through micropores;

before the liquid to be measured is injected into the front pool 120, pressure is provided to the front pool 120 or the rear pool so that micropores are unblocked, which specifically comprises: the micropores are left open by providing pressure to the front cell 120 or the back cell by the pressure in the pressure chamber 210 of the pressure build-up assembly; or the micro-pores are left open by providing pressure to the anterior chamber 120 or the posterior chamber through the syringe 220 of the pressurizing assembly.

A thirteenth embodiment, please refer to fig. 1 to 13 together, which provides a POCT blood cell analyzer, the POCT blood cell analyzer includes a detection seat 300, a human-computer interaction module and a processor, the detection seat 300 is used for receiving the package of the reagent kit 20, at least two item detection pools are disposed on the reagent kit 20, an auxiliary detector for detecting items corresponding to the item detection pools is disposed in the detection seat 300, the human-computer interaction module is in communication connection with the detection seat 300 and is used for selecting detection items, the human-computer interaction module is a combination of a touch screen or a display screen 11 and mechanical keys, and the processor is in signal connection with the detection seat 300 and the human-computer interaction module and is used for receiving detection signals of the detection items and the auxiliary detector and comparing whether the detection items and the detection signals correspond to each other, if so, the subsequent operation can be normally performed, and if not, the correct reagent kit 20 needs to be replaced or the correct detection items need to be reselected. The item detection cells are detachably mounted on the reagent cartridge 20, and the auxiliary detector is used for detecting whether the corresponding item detection cells exist.

The auxiliary detector comprises an optical detection assembly 330, the optical detection assembly 330 comprises a light emitting assembly and a light receiving assembly, and an optical signal obtained by the light receiving assembly is used for assisting in judging whether an item detection pool on the reagent kit 20 corresponds to a selected detection item.

The auxiliary detector includes a tact switch 334, and a signal obtained by the tact switch 334 is used to assist in determining whether the item detection pool on the reagent cartridge 20 corresponds to the selected detection item.

The auxiliary detector comprises an image recognition base 610 and an image recognition device 620 which are oppositely arranged at intervals, and signals obtained by the image recognition base 610 and the image recognition device 620 are used for assisting in judging whether the item detection pool on the reagent box 20 corresponds to the selected detection item.

The POCT blood cell analyzer further includes a code scanner for acquiring label information corresponding to the reagent kit 20, wherein the label information may include information such as name, type, and production time.

The present embodiment further provides a method for using the POCT blood cell analyzer, which includes the following steps:

selecting an item to be detected in a man-machine interaction module;

acquiring corresponding detection position information of the kit 20;

and judging whether the detection position information corresponds to the selected item to be detected or not through the processor, outputting a corresponding prompt if the detection position information does not correspond to the selected item to be detected, normally performing subsequent operation if the detection position information corresponds to the selected item to be detected, and replacing the correct kit 20 or reselecting the correct detection item if the detection position information does not correspond to the selected item to be detected.

In the step of acquiring the corresponding detection bit information of the reagent kit 20, the corresponding detection bit information of the reagent kit 20 includes a scattered light signal, a transmitted light signal or an electric signal triggered by a microswitch.

Wherein the items to be tested comprise at least one of blood routine tests, CRP tests, SAA tests and blood cell classification tests.

acquiring item detection information supported by the kit 20 through an auxiliary detector;

selecting an item to be detected in a man-machine interaction module;

and judging whether the item detection information supported by the kit 20 corresponds to the selected item to be detected, and if not, outputting a corresponding prompt.

The scheme provided by the embodiment compares the item which the user wants to detect with the detected item which can be actually supported by the kit before detection, and can better play a fool-proof effect.

In a fourteenth embodiment, referring to fig. 1 to 13, the present embodiment provides a POCT blood cell analyzer, which includes a housing 10, a detection seat 300, a pipette 400, a first conduit 211, and an anti-folding member 212.

The test socket 300 can extend into or out of the housing 10 for receiving the reagent cartridge 20 provided with the impedance test cell, and the test socket 300 is provided with a power supply (e.g., a conductive holder 303 shown in fig. 9) for matching impedance test.

One end of the first conduit 211 is in air path communication with the pipette 400, the other end of the first conduit 211 is in air path communication with a pressure build-up system (for example, the second injector 223 in fig. 5), the pressure build-up system may include at least one of the injector 220, the pressure chamber 210, the solenoid valves (SV 1, SV2, SV 3), the multi-way joint, and the manifold plate, and the folding prevention piece 212 is combined with the first conduit 211 to prevent the first conduit 211 from being folded excessively to block the internal air guide channel of the first conduit 211. The inner diameter of the first conduit 211 is relatively thin, and may be generally 0.5 mm to 1.5 mm, and is easily over-bent and blocked in practical use.

The anti-folding member 212 may be a sleeve sleeved on the outer periphery of the first conduit 211, the hardness of the sleeve is less than or equal to the hardness of the first conduit 211, of course, the hardness of the sleeve may also be greater than the hardness of the first conduit 211, after the sleeve is sleeved on the outer periphery of the first conduit 211, the overall hardness of the first conduit 211 and the sleeve is relatively increased, the hardness of the first conduit 211 is relatively increased, the deformation of the first conduit 211 itself is relatively smaller when the air pressure in the first conduit 211 changes, and further the precision when a sample is sucked may be improved, the wall thickness of the sleeve may be greater than or equal to the wall thickness of the first conduit 211, and the anti-bending capability of the sleeve may be less than or equal to the anti-bending capability of the first conduit 211, thereby reducing the abrasion of the sleeve to the first conduit 211, and simultaneously preventing the first conduit 211 from being excessively bent to block the internal air guide channel of the first conduit 211. The section of the sleeve is circular or C-shaped. The anti-folding member 212 may also be a spring sleeved on the outer circumference of the first guide tube 211. The folding prevention piece 212 may also be a strap wrapped around the first conduit 211, and the strap may be a cloth strap, a plastic strap, or a metal strap.

The first conduit 211 is connected at both ends thereof to the pipette 400 and the second syringe 223, respectively. The end of the first conduit 211 is provided with a joint part, which can be arranged at one end or two ends of the first conduit 211, and the joint part can be specifically a clamp, a clamp joint, a flanging joint, a straight joint or a threaded joint for gas path sealing connection.

The POCT blood cell analyzer provided by the embodiment can better avoid the occurrence of the condition of air passage obstruction and improve the stability of the analyzer due to the anti-folding piece 212 arranged on the periphery of the relatively thin first conduit 211.

In a fifteenth embodiment, referring to fig. 1 to 13, the POCT blood cell analyzer includes a housing 10, a detection seat 300, a liquid-moving device 400, a pressure-building system, a first conduit 211, and a second conduit 221.

The two ends of the first conduit 211 are respectively connected with the pipette 400 and the second syringe 223, the second conduit 221 connects the syringe 220, the pressure chamber 210 and the solenoid valves (SV 1, SV2 and SV 3), the inner diameter of the first conduit 211 (i.e. the T3 pipeline in fig. 5) is smaller than or equal to the inner diameter of the second conduit 221 (i.e. the pipelines in fig. 5 except T3 and T4), and the hardness of the first conduit 211 is larger than that of the second conduit 221. The first conduit 211 mainly controls the accuracy of sample sucking or pushing, and requires a small error due to gas compression, so that the first conduit has a large hardness and a relatively small inner diameter, while the second conduit 221 mainly builds pressure, and requires a rapid pressure, so that the inner diameter can be relatively large.

The ratio of the inner diameter of the first guide duct 211 to the inner diameter of the second guide duct 221 may be greater than 0.5 and less than or equal to 1. The ratio of the length of the first conduit 211 to the length of the second conduit 221 is greater than 1 and less than or equal to 1.5, and the relatively long length of the first conduit 211 may facilitate installation of a fitting (e.g., a bail fitting). That is, the first conduit 211 is relatively thin and stiff, and the second conduit 221 is relatively thick and soft.

As shown in fig. 5, the POCT blood cell analyzer further includes a folding prevention member 212, and the folding prevention member 212 is combined with the first guide tube 211 to prevent the first guide tube 211 from being excessively folded to block the internal air guide passage of the first guide tube 211.

The anti-folding member 212 may be a sleeve or a spring sleeved on the outer circumference of the first guiding tube 211. The hardness of the sleeve is less than or equal to that of the first conduit 211, certainly, the hardness of the sleeve can also be greater than that of the first conduit 211, after the sleeve is sleeved on the outer periphery of the first conduit 211, the overall hardness of the first conduit 211 and the sleeve is relatively increased, the hardness of the first conduit 211 is relatively greater, the deformation of the first conduit 211 is relatively smaller when the air pressure in the first conduit 211 changes, the precision during suction and discharge operation can be further improved, the wall thickness of the sleeve can be greater than or equal to that of the first conduit 211, and the bending resistance of the sleeve can be less than or equal to that of the first conduit 211. The section of the sleeve is circular or C-shaped. The anti-folding member 212 may also be a band wrapped around the first conduit 211. The bandage is a cloth bandage, a plastic bandage or a metal bandage.

The hardness of the first guide pipe 211 is greater than or equal to that of the second guide pipe 221, the hardness of the first guide pipe 211 is relatively greater, and the deformation of the first guide pipe 211 is relatively smaller when the air pressure in the first guide pipe 211 changes, so that the precision during sample suction can be improved; the wall thickness of the first conduit 211 is less than or equal to the wall thickness of the second conduit 221; the bending resistance of the first guide duct 211 is less than or equal to that of the second guide duct 221. This embodiment corresponds according to two kinds of different demands and carries out internal diameter, hardness apolegamy, and the satisfying requirement that can be pertinence realizes accurate, the high efficiency of instrument to it has to prevent a 212 to design to thinner first pipe 211, and the condition that the gas circuit is unable to appear can be better avoided, has further improved instrument stability.

In a sixteenth embodiment, referring to fig. 1 to 14, a method for using a POCT blood cell analyzer is provided, which includes the following steps:

s10, receiving a starting trigger signal, specifically, a user presses a mechanical key to switch on a power supply;

s11, performing hardware self-check, namely detecting and feeding back voltages and currents output by ports on a circuit board;

s12, judging whether the pipettor is loaded with an installation head or not, wherein the installation head can be a tip head or a puncture head through a detection element such as a correlation optical coupler or a reflection optical coupler;

s18, if yes, the liquid moving machine carries out mounting head unloading, and unloading positions can be specific positions in the instrument or certain pool positions on the reagent kit 20 in the instrument;

and S13, if not, initializing the whole machine, namely performing zero reset or zero confirmation and pressure zero reset on each moving part, loading the received kit 20 after initialization is completed, performing full-automatic sample pretreatment (automatic sample mixing and corresponding reagents) in the POCT blood cell analyzer, and performing sample detection after the automatic sample pretreatment is completed.

Wherein, the pipettor includes after carrying out the mounting head uninstallation:

s19, taking the detection seat 300 out of the warehouse for taking out the reagent box 20, wherein in this case, the power is cut off accidentally usually in the last use, and the mounting head and the reagent box 20 are still left in the instrument;

s20, the detection seat 300 returns to the warehouse and then carries out the whole machine initialization step, which is to ensure that no external element (a mounting head and a reagent box 20) exists in the machine, and then the subsequent startup process action is not influenced by the existence of the tip head or the reagent box 20.

In the step of judging whether the pipettor is loaded with the mounting head, the method further comprises: whether the reagent kit 20 is installed in the detection seat 300 is judged, generally speaking, if power is cut off accidentally, the reagent kit 20 and the installation head exist in the instrument at the same time, so that whether the installation head exists in the instrument or not can be judged only, whether the installation head exists in the instrument or not and whether the reagent kit 20 exists in the instrument or not can be judged at the same time, wherein the judgment of the reagent kit 20 can be judged by a mode of a light touch switch and the like in the detection seat 300.

After the step of initializing the whole machine, the method also comprises the following steps:

and S14, performing pressure detection, and judging whether the pressure in the air path pipeline reaches a threshold range, wherein one, two or more pressure chambers 210 can be arranged in the POCT blood cell analyzer provided by the application, positive pressure or negative pressure is established through an injector and reaches a certain pressure value range, specifically, a pressure self-detection process is performed after initialization, the pressure self-detection needs to establish negative pressure with a certain value, the pressure can be built-25-30 kpa, and the pressure building process depends on the electromagnetic valve, the injector, the pressure sensor, the pressure chambers 210 and the air path pipeline. The pressure building process is as follows: in the pressure building process, the pressure sensor detects the pressure of the pressure chamber 210 at any time, firstly, the injector motor drives the injector to pull outwards at the maximum speed, and meanwhile, the electromagnetic valve is opened to build negative pressure operation, at the moment, the injector is directly communicated with the pressure chamber 210, no other air vent is opened in the pressure chamber 210, and negative pressure can be built when the injector pulls outwards. And if the motor of the injector moves to the maximum stroke, namely after the injector is pulled to the maximum range, the target pressure is not built, closing the electromagnetic valve, initializing the injector at the maximum speed by using the maximum distance, opening the electromagnetic valve after the initialization is finished, simultaneously continuing pulling the injector outwards to build the pressure until the target pressure is reached, and reporting a pressure building fault if the pressure building construction period is finished and the target pressure is not reached. At this point, the build-up of pressure is complete. The pressure self-check is continued, and when the target pressure is established, the pressure sensor opens the solenoid valve to communicate the interior of the pressure chamber 210 with the air and release the negative pressure if it detects that the target pressure is normal. And simple blank test is carried out after the pressure self-test, the kit 20 is not required to be placed, and the signal under the no-load condition is directly measured to ensure that the POCT blood cell analyzer has normal test performance. The main measurement process is performed next.

And S15, performing signal detection, including judging a detection signal after the electromagnetic shielding assembly is matched, further judging the electromagnetic shielding effect and judging whether current and voltage signals are in a threshold range, wherein the signal detection can comprise various photoelectric signals and the like, and also including simulating the detection of an impedance method after the metal shielding cover and the metal shielding seat are combined and sealed, and judging the shielding effect of the metal shielding cover and the metal shielding seat or the signal quality of the circuit board card per se according to an output result.

And S16, entering a standby state, wherein the power-off or the detection can be selected.

Specifically, the step of entering the standby state comprises thereafter:

s17, receiving a shutdown trigger signal, namely pressing a shutdown key by a user, wherein in order to prevent misoperation, the method can be set to display whether to determine shutdown or not on a screen after the user presses the shutdown key;

s21, judging whether the reagent kit 20 exists or not, wherein the judgment of whether the reagent kit 20 exists or not can be carried out through a tact switch in the detection seat 300;

s22, if the reagent kit 20 is judged to be the reagent kit, the state of the door is further judged;

s24, if the door is judged to be in an open state, prompting to take out the reagent kit 20 and returning to the step of judging whether the reagent kit 20 exists or not;

s23, if the bin gate is judged to be in a closed state, opening the bin gate and prompting to take out the reagent kit 20 and then returning to the step of judging whether the reagent kit 20 exists or not;

s25, if the kit 20 is judged to be absent, further judging the door state;

s26, if the bin gate is judged to be in an open state, closing the bin gate and prompting to close the power supply;

and S27, if the bin gate is judged to be in a closed state, prompting to close the power supply.

The step of entering the standby state then comprises:

s31, receiving sample ID setting and measurement mode setting, specifically, providing options such as mode setting on a screen, and providing some selectable items for a user to select, such as CRP detection, SAA detection, blood detection, etc.;

s32, receiving a test starting trigger signal, namely a touch key or an entity key can be provided on a screen or an instrument to generate the test starting trigger signal;

s33, checking whether the information to be detected (such as reagent information or code scanning comparison/IC card reading comparison) is matched with the measurement mode through popup prompting, if not, clicking to cancel (S46) and returning to the step S31 again, and receiving sample ID setting and measurement mode setting;

s34, clicking to confirm if the matching is performed;

s35, automatically opening a bin door;

s36, prompting to put the kit in a pop-up window mode and the like;

s37, clicking to determine after the kit is put into the kit;

s38, detecting whether the kit 20 exists or not, and if not, returning to the step S36 to prompt the user to put in the kit 20;

s39, if yes, automatically closing the bin gate;

and S40, carrying out an automatic liquid preparation test, wherein the specific flow of the automatic liquid preparation test can refer to the embodiment.

S41, displaying the test result, specifically displaying the test result through a display screen or outputting the test result through a printing device;

s42, click determination;

s43, automatically opening a bin gate and prompting to take out the reagent kit 20;

s44, the user clicks and determines after taking out the kit 20;

and S45, automatically closing the bin gate and entering a standby state.

The POCT blood cell analyzer provided by the embodiment is reasonable and orderly in use method, and can be used for rapidly detecting a single item or simultaneously detecting a plurality of items.

The embodiment also provides a POCT blood cell analyzer, which includes a housing 10 and a pipette 400 of the detection seat 300.

The detection seat 300 can extend into or out of the shell 10, is used for receiving the reagent kit 20 to be loaded, and reciprocates between a reagent kit 20 loading station and a sample detection station; and a pipette 400 disposed in the housing 10 and above the detection seat 300, for performing an automated sample pretreatment on the reagent cartridge 20.

The kit 20 comprises a front pool 120 and a back pool which are communicated through a micropore, and the POCT blood cell analyzer further comprises a pressure building system which is arranged in the shell 10 and is used for enabling the liquid to be detected to flow from the front pool 120 to the back pool for impedance detection.

The POCT blood cell analyzer further comprises metal shielding seats (320, 321 and 322) used for being covered with the detection seat 300, the kit 20 comprises a pressure action cavity 140 communicated with the rear pool, the metal shielding seats (320, 321 and 322) are provided with air pressure connecting devices 510 connected with a pressure building system, and the air pressure connecting devices 510 are in sealing butt joint with the pressure action cavity 140 when the metal shielding seats (320, 321 and 322) are covered with the detection seat 300.

The pipette 400 is provided with a detection member (for example, an optical coupler 405 shown in fig. 6), the pipette 400 includes an air duct 401 and an outer sleeve 402 sleeved on the periphery of the air duct 401, the air duct 401 is used for loading an installation head installed on the reagent kit 20, when the air duct 401 loads the installation head, the air duct 401 is inserted into the installation head, so that the installation head pushes the outer sleeve 402 to generate position change along the axial direction of the air duct 401, and the detection member is used for detecting the position of the outer sleeve 402 to further determine whether the installation head is sleeved on the air duct 401.

Pipette 400 further includes a head retracting mechanism (referred to above as a blocker 4031) for stopping the raising of the overtube 402 when the airway tube 401 is raised so that the overtube 402 unloads the mounting head.

The head withdrawing mechanism is an electromagnet, a motor or a U-shaped opening baffle which is arranged in the axial direction or the radial direction relative to the air guide pipe 401.

The pressure building system comprises a pressure chamber 210, a first injector 222 and a second injector 223 which are arranged in a linkage mode, wherein the first injector 222 is used for building positive pressure or negative pressure for the pressure chamber 210, and the second injector 223 is communicated with the pipette 400 and used for sucking a reagent and/or a sample by taking an inner cavity of the mounting head as a transfer position in liquid when the pipette 400 is connected with the mounting head in a sleeved mode.

The POCT blood cell analyzer includes a first guide tube 211 and a second guide tube 221, the first guide tube 211 is connected between a second syringe 223 and the pipette 400, the second guide tube 221 is used for connecting the syringe and the pressure chamber 210, and the inner diameter of the first guide tube 211 is smaller than that of the second guide tube 221.

The first guide tube 211 is provided at the outer circumference thereof with an anti-folding member 212, and the anti-folding member 212 is a sleeve, a spring or a strap.

The embodiment further provides a POCT blood cell analyzer, which includes a detection seat 300, a pipette 400 disposed above the detection seat 300, and a pressure buildup system, wherein the detection seat 300 is used for receiving the reagent kit 20, the reagent kit 20 is provided with a front cell 120 and a rear cell which are communicated through a micropore and are provided with a reagent, a sample, and a mounting head, and the pressure buildup system includes a pressure chamber 210, an air pressure connecting device 510, a first injector 222, a second injector 223, a driving member 224, a first solenoid valve SV1, a second solenoid valve SV2, and a third solenoid valve SV3.

The pneumatic connecting device 510 is connected with the pressure chamber 210 and is used for applying positive pressure or negative pressure to the front pool 120 or the rear pool to realize that the liquid in the front pool 120 flows to the rear pool; the first syringe 222 is communicated with the pressure chamber 210/the pipette 400, and is used for establishing positive pressure or negative pressure for the pressure chamber 210, assisting the pipette 400 to perform pipetting, or performing bubbling and uniform mixing on liquid in the forebay 120; the second syringe 223 is in communication with the pipettor 400 for pipetting; the drive member is used to drive the first syringe 222 and the second syringe 223 simultaneously; a first solenoid valve SV1 selectively communicates the first syringe 222 with the pressure chamber 210 and the second syringe 223; the second solenoid valve SV2 selectively communicates the first solenoid valve with the pressure chamber 210 and the outside atmosphere; the third solenoid valve SV3 selectively communicates the pneumatic connection with the pressure chamber 210.

The application also provides a kit 20, and the kit 20 comprises a box body 100, tip head accommodating tanks (101, 102, 103), a mounting head accommodating tank, a sample accommodating tank 105, a hemolytic agent tank 106, a rectangular insertion hole 107, a circular insertion hole 108, a front tank 120, a mounting cavity, a mounting seat 160, a micro-porous sheet 170, electrodes 121 of the front tank 120, rear tank electrodes 165, a pressure acting cavity 140, tip heads (201, 202, 203), a puncture head 204, a sample tube 205, a hemolytic agent container 206, a first tank body 207, a second tank body 208 and the like.

The following will specifically describe each embodiment of the kit 20 by way of examples.

A seventeenth embodiment, please refer to fig. 15 and 20 together, in which the embodiment of the present invention provides a microporous sheet 170, the microporous sheet 170 includes a sheet 171, the sheet 171 is provided with micropores 172 allowing cells to pass through one by one, the micropores 172 can be made into different specifications according to cells with different particle sizes, the sheet 171 is a plastic sheet or a ceramic sheet, the mechanical strength of the plastic sheet or the ceramic sheet is relatively weak, the sheet 171 of the embodiment of the present invention is further provided with a reinforcing part 173 to ensure the mechanical strength of the microporous sheet 170, and at the same time, the microporous sheet 170 is convenient to install, when the microporous sheet 170 is installed, the micropores 172 are not easily polluted or worn by contact, the material cost of the plastic sheet or the ceramic sheet is relatively cheap, and the microporous sheet can be used as a disposable product without using expensive materials capable of being repeatedly cleaned and used.

Sheet 171 has first 175 and second 176 opposite surfaces, and first 175 and/or second 176 surfaces are provided with reinforcements 173.

The reinforcement 173 may be disposed proximate to an edge of the sheet 171. The reinforcement 173 may be configured as a torus, which may be a continuous integral torus or a torus formed by a plurality of hulls around the torus, with or without an outer edge of the torus coinciding with an outer edge of the sheet 171.

The convex ring is connected to the first surface 175 and/or the second surface 176 of the sheet 171 by a vertical surface, an inclined surface or an arc surface, wherein the inclined surface or the arc surface can further reduce sample residue and improve detection accuracy.

The first surface 175 of the sheet 171 is provided with a concave drainage part 174 at the periphery of the micro-holes 172, and the concave drainage part 174 may be spherical or conical.

The ratio of the thickness of the protruding ring to the thickness of the sheet body 171 is 0.2-2, preferably, the ratio of the width of the protruding ring to the radius of the sheet body 171 is less than or equal to 1, if the thickness of the protruding ring is too small, the protruding ring cannot play a good role in reinforcing mechanical strength, if the thickness of the protruding ring is too large, materials are wasted, and if the thickness of the protruding ring is too large, the wall thickness of the micropores 172 of the microporous sheet 170 is also difficult to control, which results in a complex manufacturing process. The ratio of the width of the convex ring to the radius of the sheet 171 is 0.2-0.8, and similarly, if the width of the convex ring is too small, the convex ring cannot provide a good mechanical strength reinforcing effect, and if the width of the convex ring is too large, the wall thickness of the micropores 172 of the microporous sheet 170 is difficult to control, which results in a complex manufacturing process, and if the width of the convex ring is too large (for example, close to the radius of the sheet 171), the axial channel of the micropores 172 is lengthened, which further causes the backflow of the sample particles to be detected, which affects the accuracy of detection when the cells pass through.

The embodiment provides a microporous sheet 170, which can improve the mechanical strength of the microporous sheet 170 by providing a reinforcing part 173, and prevent the microporous sheet 170 made of plastic material or ceramic material from being easily deformed when being assembled, and also provides a kit 20 including the microporous sheet 170, and the specific structure of the kit 20 is referred to below.

Eighteenth embodiment, referring to fig. 15 and 20, the present application provides a reagent kit 20, wherein the reagent kit 20 includes a kit body 100, a mounting seat 160, and a microporous sheet 170.

The cartridge 100 includes a front cell 120, the cartridge 100 is provided with electrodes 121 of the front cell 120 corresponding to the front cell 120, and the front cell 120 may be provided with two groups, which are respectively used for performing WBC (white blood cell) detection and RBC (red blood cell) detection in cooperation.

As shown in fig. 6, the mounting base 160 is connected to the box 100, and the mounting base 160 has an axial drainage chamber 167 (the axial drainage chamber 167 may also be referred to as a rear cell), and a rear cell electrode 165 extending to the axial drainage chamber 167.

As shown in fig. 1, 2 and 6, the microporous sheet 170 is provided with micropores 172 allowing cells to pass through one by one, the microporous sheet 170 is disposed between the front cell 120 and the axial drainage chamber 167, the front cell 120 and the axial drainage chamber 167 are communicated through the micropores 172, and the electrodes 121 of the front cell 120 and the electrodes 165 of the rear cell are respectively spaced at both sides of the microporous sheet 170.

The mounting socket 160 is detachably coupled to the cartridge 100.

In an embodiment, the electrodes 121 of the front cell 120 may be integrally injection molded with the cartridge body 100 or may be removably attached. The rear cell electrode 165 and the mounting seat 160 can be integrally injection-molded or detachably connected, the front cell 120 electrode 121 and/or the rear cell electrode 165 are columnar electrodes, and the length of the rear cell electrode 165 is greater than or equal to that of the front cell 120 electrode 121. The microporous sheet 170 may be integrally injection molded or removably attached to the case 100, or the microporous sheet 170 may be integrally injection molded or removably attached to the mounting cup 160.

The box body 100 is provided with a mounting cavity 130, and the assembling seat 160 is in snap fit, threaded fit, interference fit, laser welding fit or bonding fit with the mounting cavity 130.

The box body 100 and/or the assembly seat 160 are plastic bodies, the front cell 120 electrode 121 is embedded in the box body 100 and is flush, protruded or recessed on the outer surface of the box body 100, the rear cell electrode 165 is embedded in the assembly seat 160 and is flush, protruded or recessed on the outer end surface of the assembly seat 160, the outer ends (i.e., two ends far away from each other) of the front cell 120 electrode 121 and the rear cell electrode 165 are used for connecting working voltage, the inner ends (i.e., two ends close to each other) of the front cell 120 electrode 121 and the rear cell electrode 165 are in contact with a sample liquid to be detected, and the axial drainage cavity 167 is filled with the sample liquid to be detected during detection.

The kit 20 further includes an inner gasket 164, wherein the inner gasket 164 is disposed between the microporous sheet 170 and the anterior chamber 120, and in particular, between the first surface 175 of the microporous sheet 170 and the anterior chamber 120, such that the sample fluid to be tested in the anterior chamber 120 can only enter the axial drainage lumen 167 through the micropores 172.

The reagent vessel 20 further comprises an outer sealing ring 166, the outer sealing ring 166 being arranged between the mounting socket 160 and the free end of the mounting chamber 130.

In one embodiment, the inner seal ring 164, the outer seal ring 166, the microporous sheet 170, and the mounting socket 160 are separate structural components.

In another embodiment, the inner seal ring 164, the outer seal ring 166, the microporous sheet 170 and the mounting seat 160 may be an integral structure to reduce the number of mounting parts, the difficulty of mounting, and the time of mounting, wherein the inner seal ring 164 and the outer seal ring 166 may be formed by injection molding using a relatively soft plastic material by a two-shot molding process.

In another embodiment, the inner seal ring 164, the outer seal ring 166, and the mounting seat 160 are an integral structure, and the microporous sheet 170 is detachably connected to the integral structure, so that the number of mounting components can be reduced, and the manufacturing accuracy and the yield of the microporous sheet 170 can be ensured. Wherein the inner seal ring 164 has a flexibility such that when the microporous sheet 170 is assembled with the integral structure, the inner seal ring 164 can be assembled behind the inner seal ring 164 by the flexibility of the inner seal ring 164, and the inner seal ring 164 still seals against the first surface 175 of the microporous sheet 170.

The reagent kit 20 provided by the embodiment has novel structure and convenient assembly.

In a nineteenth embodiment, referring to fig. 15 and 20, the present embodiment provides a mounting base 160, wherein the mounting base 160 includes a mounting cylinder 168 and a rear cell electrode 165.

The mounting cylinder 168 is provided with an axial drainage cavity 167 and a radial liquid outlet slot 1611 (see fig. 16) which are communicated with each other, wherein the radial liquid outlet slot 1611 is used for discharging gas or liquid in the axial drainage cavity 167; the rear cell electrode 165 is connected to the mounting cylinder 168 and extends into the axial drainage lumen 167.

A first preset distance is formed between the inner end of the radial liquid outlet groove 1611 and the inner end of the assembly cylinder 168, a second preset distance is formed between the inner end of the rear cell electrode 165 and the inner end of the assembly cylinder 168, and the first preset distance is smaller than or equal to the second preset distance. When first preset distance is less than second preset distance, the bubble in axial drainage chamber 167 discharges axial drainage chamber 167 more easily, if remain the bubble in axial drainage chamber 167, can influence and detect the precision. The inner end is referred to the inside of the cartridge 100, and when the forebay 120 is referred to, the end pointing to the forebay 120 is the inner end, and the end departing from the forebay 120 is the outer end, for example, the inner end of the mounting cavity 130 is communicated with the forebay 120 through the via hole 132, and the outer end of the mounting cavity 130 is an open end for receiving the mounting seat 160.

The axial length of the radial exit slot 1611 in the axial drainage lumen 167 is greater than or equal to the length of the rear cell electrode 165 that extends into the axial drainage lumen 167. Wherein the inner end of rear cell electrode 165 may or may not extend into axial drainage lumen 167, i.e., the inner end of rear cell electrode 165 may protrude, be flush, or be recessed at the bottom surface of axial drainage lumen 167.

Further, the outer surface of the mounting cylinder 168 is provided with a recessed area communicated with the radial liquid outlet groove 1611 to form a flow guide groove 1612, the flow guide groove 1612 can enable gas or liquid in the axial flow guide cavity 167 to flow away through the flow guide groove 1612 obliquely above after coming out of the radial liquid outlet groove 1611 directly above, so that the two mounting bases 160 can share one pressure acting cavity 140 communicated with the two mounting bases 160, and the pressure acting cavity 140 is communicated with the mounting cavity 130 through a through hole 133 (which may be in a sector shape as shown in fig. 17), and further communicated with the axial flow guide cavity 167 sequentially through the flow guide groove 1612 and the radial liquid outlet groove 1611.

The inner end of the fitting cylinder 168 is provided with a micro-porous sheet 170 for allowing cells to pass therethrough one by one, and the specific structure of the micro-porous sheet 170 can be referred to the previous embodiment.

The distance from the inner end of the rear cell electrode 165 to the microporous sheet 170 is a third preset distance which is 0.2-2 times the axial length of the axial drainage cavity 167, and experiments prove that in the range, better signal precision can be obtained during impedance detection.

The inner end of the mounting cylinder 168 is provided with a sinking platform 1613, the microporous sheet 170 is matched and connected with the sinking platform 1613, and the reinforcing part 173 of the microporous sheet 170 is abutted against the sinking platform 1613.

As shown in fig. 16, the mounting tube 168 includes a rear Chi Tong, an end plate 162 and an outer tube 163 which are integrally connected, the rear Chi Tong is provided with an axial flow guide cavity 167 and a radial liquid outlet groove 1611, the end plate 162 radially connects the rear Chi Tong and the outer tube 163, and the outer tube 163 is sleeved on the periphery of the rear Chi Tong at intervals.

The radial drain channel 1611 may be circular, kidney-shaped or rectangular.

The application also provides a reagent kit 20, the reagent kit 20 comprises a box body 100 and the assembling seat 160, the box body 100 is provided with a front pool 120 and a mounting cavity 130 which are communicated, the assembling cylinder 168 is connected with the mounting cavity 130, and the front pool 120 is communicated with the axial drainage cavity 167 through the micropores 172 of the microporous sheet 170.

The reagent kit 20 and the assembly seat 160 thereof provided by the embodiment can conveniently discharge the bubbles in the assembly cylinder 168 by limiting the distance between the inner end of the radial liquid outlet slot 1611 and the inner end of the rear cell electrode 165 and the inner end of the assembly cylinder 168, and prevent the bubbles from remaining in the assembly cylinder 168 to influence the detection precision in the impedance method detection.

In a twentieth embodiment, referring to fig. 15 and 20, an assembly base 160 according to an embodiment of the present invention includes an assembly cylinder 168 and a rear cell electrode 165, the rear cell electrode 165 is a cylindrical electrode, and the rear cell electrode 165 is connected to the assembly cylinder 168.

The assembling barrel 168 is provided with a radial liquid outlet groove 1611 and a buckling part 1631, the buckling part 1631 and the radial liquid outlet groove 1611 are arranged in an aligned mode, the buckling part 1631 can be a buckling hole or a buckling lug, correspondingly, the outer surface of the mounting cavity 130 is provided with a buckling block 131 corresponding to the buckling hole, and the opening directions of one of the buckling parts 1631 and the radial liquid outlet groove 1611 are consistent and arranged in an aligned mode, so that the die can be conveniently pulled out from the same direction during injection molding of the die.

The assembly barrel 168 is provided with an axial drainage cavity 167, the axial drainage cavity 167 is communicated with the radial liquid outlet groove 1611, and the rear pool electrode 165 extends to the axial drainage cavity 167.

The rear cell electrode 165 may be embedded in the mounting seat 160 by injection molding and flush, protruded or recessed on the outer end surface of the mounting seat 160.

In the embodiment of the application, the assembly cylinder 168 comprises a rear Chi Tong, an end plate 162 and an outer cylinder 163 which are integrally connected, a radial liquid outlet groove 1611 and an axial flow guide cavity 167 are arranged on a rear Chi Tong, and the end plate 162 radially connects a rear Chi Tong and the outer cylinder 163.

The outer barrel 163 may extend axially with a plurality of positioning protrusions 1632 arranged at intervals, and the fastening portion 1631 is arranged corresponding to the positioning protrusions 1632.

A first preset distance is formed between the inner end of the radial liquid outlet groove 1611 and the inner end of the assembly cylinder 168, a second preset distance is formed between the inner end of the rear cell electrode 165 and the inner end of the assembly cylinder 168, and the first preset distance is smaller than or equal to the second preset distance.

The rear cell electrode 165 and the mounting cylinder 168 may be integrally formed or removably attached.

The inner end of the rear Chi Tong is provided with a sink 1613 for mounting the microporous sheet 170 that allows cells to pass through one by one.

The application also provides a reagent kit 20, the reagent kit 20 comprises a box body 100 and the assembling seat 160, the box body 100 is provided with a front pool 120 and a mounting cavity 130 which are communicated, the assembling cylinder 168 of the assembling seat 160 is connected with the mounting cavity 130, and the front pool 120 is communicated with the axial drainage cavity 167 through micropores 172 of a microporous sheet 170.

The reagent cartridge 20 and the assembling seat 160 thereof provided in this embodiment can facilitate the mold drawing operation after the mold forming by aligning the radial liquid outlet slot 1611 and the fastening part 1631.

In a twenty-first embodiment, referring to fig. 15 and 20, an embodiment of the present invention provides a mounting base 160, wherein the mounting base 160 includes a mounting cylinder 168 and a rear cell electrode 165.

The assembling cylinder 168 is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611 which are communicated with each other, a recessed area communicated with the radial liquid outlet groove 1611 is formed on the outer surface of the assembling cylinder 168 to form a flow guide groove 1612, the flow guide groove 1612 can enable gas or liquid in the axial drainage cavity 167 to smoothly flow to the pressure action cavity 140 through the flow guide groove 1612 (for example, obliquely upward) after coming out of the radial liquid outlet groove 1611 (for example, directly upward), so that the two assembling bases 160 can share one pressure action cavity 140 communicated with the two assembling bases 160, and the pressure action cavity 140 is communicated with the mounting cavity 130 through a through hole 133 (which can be in a fan shape as shown in fig. 17) and is further communicated with the axial drainage cavity 167 through the flow guide groove 1612 and the radial liquid outlet groove 1611 in sequence.

The rear cell electrode 165 is connected to the mounting cylinder 168 and extends into the axial drainage lumen 167.

The guiding gutter 1612 sets up along the surface circumference of assembly section of thick bamboo 168, and the guiding gutter 1612 is the arc structure, and the central angle of arc structure is 0 ~ 360, can make more smooth and easy flow direction pressure effect chamber 140 of sample liquid. The depth of the concave of the flow guide groove 1612 is 1/5-4/5 of the wall thickness of the assembly cylinder 168, so that the flow guide groove 1612 has certain mechanical strength and is not easy to damage while the liquid is ensured to flow out smoothly.

The radial liquid outlet groove 1611 is connected with the flow guide groove 1612 through a transition surface, and the transition surface is a vertical plane, an inclined plane or an arc surface.

Rear cell electrode 165 is embedded in mounting base 160 and is flush, raised or recessed on the outer end face of mounting base 160.

A first preset distance is reserved between the inner end of the radial liquid outlet groove 1611 and the inner end of the assembling cylinder 168; a second preset distance is formed between the inner end of the rear cell electrode 165 and the inner end of the assembly cylinder 168; the first preset distance is less than or equal to the second preset distance.

As shown in fig. 16, the mounting tube 168 comprises a rear Chi Tong, an end plate 162 and an outer tube 163 which are integrally connected, a rear Chi Tong is provided with a radial liquid outlet groove 1611 and an axial flow guide cavity 167, and the end plate 162 radially connects the rear Chi Tong and the outer tube 163.

A plurality of positioning protrusions 1632 are axially extended from the outer cylinder 163, and the fastening portion 1631 is disposed corresponding to the positioning protrusions 1632.

The application also provides a reagent kit 20, the reagent kit 20 comprises a box body 100 and the assembling seat 160, the box body 100 is provided with a forebay 120 and a mounting cavity 130 which are communicated; the mounting cylinder 168 of the mounting socket 160 is connected to the mounting chamber 130, and the front cell 120 is in communication with the axial drainage chamber 167 via the pores 172 of the microporous sheet 170.

The reagent kit 20 and the assembly base 160 thereof provided by this embodiment can facilitate the two assembly bases 160 to share one pressure acting cavity 140 communicated with the two assembly bases 160 by providing the radial liquid outlet slot 1611 on the assembly barrel 168 and forming a recessed area on the outer surface of the radial liquid outlet slot 1611 to form the flow guide slot 1612, so as to facilitate the negative pressure drainage of the pressure acting cavity 140.

In a twenty-second embodiment, referring to fig. 15 and 20, an embodiment of the present invention provides a mounting base 160, wherein the mounting base 160 includes a mounting cylinder 168 and a rear cell electrode 165.

The assembly cylinder 168 is a plastic assembly cylinder and is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611 which are communicated with each other, and the radial liquid outlet groove 1611 is used for discharging gas or liquid in the axial drainage cavity 167; the rear cell electrode 165 is connected to the mounting cylinder 168 and extends into the axial drainage lumen 167.

The outer surface of the mounting cylinder 168 is provided with a recessed area communicated with the radial liquid outlet groove 1611 to form a flow guide groove 1612, the flow guide groove 1612 can enable gas or liquid in the axial flow guide cavity 167 to flow out through the radial liquid outlet groove 1611 (for example, right above) and then flow to the pressure action cavity 140 smoothly through the flow guide groove 1612 (for example, obliquely above), so that the two mounting bases 160 can share one pressure action cavity 140 communicated with the two mounting bases 160, the pressure action cavity 140 is communicated with the mounting cavity 130 through a through hole 133 (which may be in a sector shape as shown in fig. 17), and further communicated with the axial flow guide cavity 167 sequentially through the flow guide groove 1612 and the radial liquid outlet groove 1611.

The assembling barrel 168 is provided with a buckling part 1631, the buckling part 1631 is aligned with the radial liquid outlet groove 1611, the buckling part 1631 can be a buckling hole, and the buckling part 1631 and the radial liquid outlet groove 1611 are aligned to enable the die to be conveniently pulled out from the same direction during injection molding of the die.

A first preset distance is reserved between the inner end of the radial liquid outlet groove 1611 and the inner end of the assembling cylinder 168; a second preset distance is formed between the inner end of the rear cell electrode 165 and the inner end of the assembly cylinder 168; the first preset distance is less than or equal to the second preset distance. When first preset distance is less than second preset distance, the bubble in the axial drainage chamber 167 discharges axial drainage chamber 167 more easily, if remain the bubble in the axial drainage chamber 167, can influence and detect the precision.

The axial length of the radial exit slots 1611 in the axial drainage lumen 167 is greater than or equal to the length of the rear cell electrode 165 extending into the axial drainage lumen 167.

The inner end of the fitting cylinder 168 is provided with a microporous sheet 170 allowing cells to pass therethrough one by one. The distance from the inner end of the rear cell electrode 165 to the microporous sheet 170 is a third predetermined distance, which is 0.2-2 times the axial length of the axial drainage lumen 167. The inner end of the mounting cylinder 168 is provided with a counter-sunk platform 1613, and the microporous sheet 170 is connected with the counter-sunk platform 1613 in a matching way.

In the embodiment of the application, the assembly barrel 168 comprises a rear Chi Tong, an end plate 162 and an outer barrel 163 which are integrally connected, the rear Chi Tong is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611, the end plate 162 radially connects the rear Chi Tong with the outer barrel 163, the outer barrel 163 is sleeved on the periphery of the rear Chi Tong at intervals, and a buckling part 1631 is arranged on the outer barrel 163.

The application also provides a reagent kit 20, the reagent kit 20 comprises a box body 100 and the assembling seat 160, the box body 100 is provided with a forebay 120 and a mounting cavity 130 which are communicated; the assembling cylinder 168 of the assembling seat 160 is connected with the mounting cavity 130, the front cell 120 is communicated with the axial drainage cavity 167 through the micropores 172 of the microporous sheet 170, the front cell 120, the mounting cavity 130, the axial drainage cavity 167, the radial liquid outlet groove 1611 and the pressure action cavity 140 jointly form a drainage channel, and the axial drainage cavity 167 can receive negative pressure to suck a sample to be tested in the front cell 120 through the micropores 172 of the microporous sheet 170.

The reagent cartridge 20 and the assembling seat 160 thereof provided by the embodiment have novel structures and are convenient to manufacture and assemble.

In a twenty-third embodiment, referring to fig. 15 and 20, an embodiment of the present disclosure provides a reagent kit 20, where the reagent kit 20 includes a box 100, a hemolytic agent pool 106 is disposed on the box 100, and a diluent pool 111 is disposed near the hemolytic agent pool 106.

At least any two of the hemolytic agent tank 106, the diluent tank 111, and the cartridge 100 are integrally connected, and at least any two of the hemolytic agent tank 106, the diluent tank 111, and the cartridge 100 are detachably connected. That is, the cartridge 100, the hemolyzing agent tank 106, and the diluent tank 111 may be of a single-piece structure or of any separate structure.

For example, the cartridge 100, the hemolytic agent pool 106, and the diluent pool 111 are three separate pieces and detachably connected to each other; alternatively, the diluent reservoir 111 and the cartridge 100 are an integral structure, and the separate hemolytic agent container 206 is detachably assembled to the hemolytic agent reservoir 106; or, the hemolytic agent pool 106 and the box body 100 are an integral structure, and a separate diluent pool test tube is detachably assembled in the diluent pool 111; alternatively, the hemolytic agent pool 106 and the diluent pool 111 are integrally connected and detachably connected to the cartridge 100.

Any two of the hemolytic agent reservoir 106, the diluent reservoir 111, and the cartridge 100 form a combination, and the combination is provided with an assembling portion (for example, an inner peripheral wall of a mounting hole provided in the cartridge 100), and another of the hemolytic agent reservoir 106, the diluent reservoir 111, and the cartridge 100 is provided with a detachable portion (for example, an outer peripheral wall of the hemolytic agent container 206 shown in fig. 15), and the detachable portion is connected to the assembling portion in a fitting manner.

The surface of the assembling portion or the disassembling portion is further provided with a protrusion 2061, and the protrusion 2061 may make the hemolytic agent container 206 abut against and tightly fit with the insertion hole provided on the cartridge 100.

In one embodiment, the cartridge 100 has two assembly positions, and the hemolytic agent pool 106 and the diluent pool 111 are respectively disposed in the two assembly positions.

In the embodiment of the present application, the cartridge 100 includes a front cell 120 and a mounting chamber 130 which are communicated with each other, the front cell 120 and the mounting chamber 130 are communicated with each other through a via hole 132 (see fig. 17 and 6), the front cell 120 is equipped with an electrode 121 of the front cell 120, the reagent cartridge 20 further includes a mounting seat 160 connected with the mounting chamber 130, the mounting seat 160 is provided with a micro-porous sheet 170 and a rear cell electrode 165, the micro-porous sheet 170 is installed at the via hole 132, the electrode 121 of the front cell 120 and the rear cell electrode 165 are respectively spaced at two sides of the micro-porous sheet 170, the mounting seat 160 includes a mounting cylinder 168, and the mounting cylinder 168 is provided with an axial drainage chamber 167 and a radial liquid outlet groove 1611.

In the embodiment of the application, the assembling cylinder 168 comprises a rear Chi Tong, an end plate 162 and an outer cylinder 163 which are integrally connected, the rear Chi Tong is provided with an axial drainage cavity 167 and a radial liquid outlet groove 1611, the end plate 162 radially connects the rear Chi Tong with the outer cylinder 163, and the outer cylinder 163 is sleeved on the periphery of the rear Chi Tong at intervals.

The volume of the diluent reservoir 111 is typically relatively large, and thus the volume of the diluent reservoir 111 is greater than or equal to the volume of the hemolytic agent reservoir 106 in the present embodiment.

The open ends of the diluent reservoir 111 and the hemolytic agent reservoir 106 are provided with sealing films to facilitate long-term preservation of the diluent hemolytic agent.

The kit 20 provided by the embodiment has a novel structure and a plurality of detachable schemes, and can meet different detection requirements.

In a twenty-fourth embodiment, referring to fig. 15 and 20, a reagent kit 20 is provided in the embodiments of the present application, where the reagent kit 20 includes a box 100 and at least one item detection cell, and the at least one item detection cell is integrally connected or detachably connected to the box 100.

The item detection pool includes a first item detection pool for performing a first item detection and a second item detection pool for performing a second item detection, and the first item detection pool and/or the second item detection pool are integrally connected or detachably connected to the cartridge 100.

The reagent cartridge 20 further includes a third item detection cell for performing a third item detection, and the third item detection cell is integrally connected or detachably connected to the cartridge body 100.

The second project detection pool and the third project detection pool are arranged on the same side edge of the first project detection pool; or the second project detection pool and the third project detection pool are respectively arranged at two sides of the first project detection pool.

The detection items of the first item detection pool, the second item detection pool and the third item detection pool can be selected from specific protein detection, biochemical detection, immunoassay and blood routine detection, for example, the blood routine detection can be performed by the cooperation of the forebay 120, the installation cavity 130, the assembly seat 160 and the pressure action cavity 140, and the specific protein detection, biochemical detection, immunoassay and the like can be performed by the cooperation of the optical detection cup.

The detection items of the first item detection pool, the second item detection pool and the third item detection pool are the same, different or not completely the same; or any one of the first item detection pool, the second item detection pool and the third item detection pool supports more than two detection items.

The cartridge 100 is provided with insertion holes which may comprise rectangular insertion holes 107 and circular insertion holes 108 through which the second item detection cells are inserted, or which are flanged for hanging fit with the insertion holes.

The second item detection cell includes a first cell body 207, a second cell body 208, and a connection 2071 connecting the first cell body 207 and the second cell body 208, the connection 2071 connecting the first cell body 207 and the second cell body 208 in an assembly with the case 100.

The first item detection pool and/or the second item detection pool is a light-transmitting plastic pool or a glass pool. The opening end of the first item detection cell and/or the second item detection cell is provided with a sealing film, and when the first item detection cell and/or the second item detection cell are/is used, the sealing film can be used after being punctured by a puncturing head 204 arranged on the box body 100.

In a twenty-fifth embodiment, please refer to fig. 15 and 20, the embodiment of the present application provides a reagent kit 20, the reagent kit 20 includes a box body 100, the box body 100 includes a forebay 120, a mounting cavity 130 and a pressure acting cavity 140, the forebay 120 is communicated with the mounting cavity 130, the pressure acting cavity 140 is vertically disposed, and the mounting cavity 130 is horizontally disposed.

The open end of the mounting cavity 130 faces the outer side surface of the box body 100, the reagent box 20 further comprises an assembling seat 160, the assembling seat 160 is connected with the mounting cavity 130, the assembling seat 160 is provided with an axial drainage cavity 167, and the axial drainage cavity 167 is communicated with the pressure acting cavity 140.

The mounting chamber 130 forms a drainage gap with the mounting seat 160, through which the axial drainage chamber 167 communicates with the pressure application chamber 140.

The assembling seat 160 is provided with a radial liquid outlet groove 1611, and the axial drainage cavity 167 is communicated with the pressure action cavity 140 through the radial liquid outlet groove 1611; the mounting block 160 is provided with a flow guide channel 1612 adjacent to the radial liquid outlet channel 1611, and the axial flow guide cavity 167 is communicated with the pressure application cavity 140 through the radial liquid outlet channel 1611 and the flow guide channel 1612.

The mounting seat 160 is provided with a microporous sheet 170 and a rear cell electrode 165, the front cell 120 is provided with a front cell 120 electrode 121, and the front cell 120 electrode 121 and the rear cell electrode 165 are respectively positioned at two sides of the microporous sheet 170 at intervals.

The mounting socket 160 is snap fit, threaded, interference fit, laser welded, or adhesively fit with the mounting cavity 130.

The kit 20 further includes an inner sealing ring 164 and an outer sealing ring 166, the inner sealing ring 164 being disposed between the microporous sheet 170 and the cartridge body 100, and the outer sealing ring 166 being disposed between the mounting cup 160 and the cartridge body 100.

In the embodiment of the present application, the forebay 120 and the installation cavity 130 are both two sets, and one pressure acting cavity 140 is communicated with the two sets of installation cavities 130.

The reagent kit 20 provided by the embodiment has a novel structure, the front pool 120, the installation cavity 130 and the pressure action cavity 140 are mutually communicated and are sequentially and vertically arranged, the open end of the pressure action cavity 140 is positioned on the upper surface of the box body 100, and the negative pressure source can be applied from the upper part of the box body 100, so that the structure of the box body 100 can be simplified, and the side surface of the box body 100 is prevented from having a more convex structure.

In a twenty-sixth embodiment, referring to fig. 15 and 20, a reagent cartridge 20 is provided in an embodiment of the present application, where the reagent cartridge 20 includes a plurality of first wells, and centers of the plurality of first wells are substantially arranged in a first straight line or a first arc line.

The reagent cartridge 20 further comprises a plurality of second wells arranged with a second straight line at the center, wherein the first straight line and the second straight line are arranged in parallel or vertically at an interval.

Or, the reagent kit 20 includes that the center is a plurality of second pond positions that the second arc line was arranged, and first arc line and second arc line are parallel interval and set up, and the arc line can be the corresponding arc line of 0 ~ 360 central angle.

The plurality of first cell sites and/or the plurality of second cell sites comprise impedance detection cells and/or optical detection cells.

The plurality of first tank positions and/or the plurality of second tank positions further comprise a diluent tank 111, and the diluent tank 111 is used for packaging diluent; the plurality of first well sites and/or the plurality of second well sites further comprise a hemolytic agent well 106, the hemolytic agent well 106 being for encapsulating hemolytic agent; the one or more first well locations and/or the plurality of second well locations further comprise a sample dilution well 112, the sample dilution well 112 being for sample dilution.

The impedance detection cell is provided with a light transmission detection window used for matching optical detection, namely, one of the front cells 120 is provided with a light transmission detection window used for matching optical detection, the front cell 120 can be integrally made of transparent plastic, and the transmittance and the smoothness of the light transmission detection window can be the same as those of the front cell 120 or higher than other parts of the front cell 120.

The plurality of first pond sites and/or the plurality of second pond sites also comprise a plurality of plug-in mounting holes.

The first plurality of well sites and/or the second plurality of well sites comprise at least one tip head receiving well (101, 102, 103) and/or a sample receiving well 105.

The first plurality of cuvettes and/or the second plurality of cuvettes include at least one puncture tip receiving cuvette 104. The application provides a kit 20 is degradable plastic kit 20, through arranging a plurality of pond position straight lines, can conveniently move the motion that the liquid device carries out shorter route when automated inspection, and wherein, move the liquid device and be arranged in shifting and mixing the liquid in each cell body.

In a twenty-seventh embodiment, referring to fig. 15 and 20 together, the reagent cartridge 20 according to the embodiment of the present invention includes a cartridge body 100, and the cartridge body 100 is provided with a detection area (e.g., an area formed by a front pool 120, a mounting cavity 130, a pressure action cavity 140, and a mounting seat 160), a reagent area (e.g., an area formed by a diluent pool 111, a hemolytic agent pool 106, and a sample accommodation pool 105), and an accessory placement area (e.g., an area formed by tip

head accommodation pools

101, 102, and 103, and a puncture head accommodation pool 104).

The cartridge 100 further comprises an expandable region (e.g., the region formed by the rectangular insertion hole 107 and the circular insertion hole 108 in the figure) disposed near the detection region, the expandable region being located at one side of the detection region, or the expandable region being located at one side of the reagent region.

The accessory placing area is arranged close to the reagent area, and the detection area is arranged close to the reagent area.

The detection zone comprises an impedance detection cell and/or an optical detection cell comprising a sensor for detecting any one of HGB, CRP, SAA parameters.

The reagent zone includes at least one of a diluent reservoir, a hemolytic agent reservoir, and a sample receiving reservoir 105.

The reagent zone comprises Chi Tiou, and the cell body zone is configured to detachably mount at least one of a diluent container, a hemolytic agent container 206, and sample holding cell 105.

The attachment placement area includes placement locations for tip heads 201, 202, 203 and/or puncture head 204.

The expandable zone includes a removable optical detection cell and/or an impedance detection cell, the optical detection cell including a sensor for detecting any one of HGB, CRP, SAA parameters.

The reagent box 20 that this application provided is degradable plastic reagent box 20, sets up through the subregion and can make things convenient for the motion that the liquid-transfering device carried out shorter route when automated inspection.

In a twenty-eighth embodiment, referring to fig. 15 and 20 together, the reagent cartridge 20 according to the embodiment of the present invention includes a cartridge body 100, wherein the cartridge body 100 is provided with a detection area (e.g., an area formed by a front pool 120, a mounting cavity 130, a pressure action cavity 140, and a mounting seat 160), a reagent area (e.g., an area formed by a diluent pool 111, a hemolytic agent pool 106, and a sample accommodation pool 105), and an accessory placement area (e.g., an area formed by tip

head accommodation pools

101, 102, and 103, and a puncture head accommodation pool 104), and the reagent area is disposed near the accessory placement area.

The cartridge 100 also includes an expandable region (e.g., the region defined by rectangular openings 107 and circular openings 108) that is positioned adjacent to the detection zone. Alternatively, the expandable region is disposed adjacent to a side of the detection region or adjacent to a side of the reagent region.

The reagent area comprises a plurality of reagent tanks which are approximately arranged in a straight line or an arc line.

The reagent zone comprises at least two tank body zones, and the at least two tank body zones comprise bottom tanks and/or bottomless insertion holes.

The accessory area comprises one or more bottomed wells for accommodating tip heads 201, 202, 203 and/or piercing heads 204.

The expandable area is provided with a circular insertion hole 108 and a rectangular insertion hole 107, the circular insertion hole 108 and the rectangular insertion hole 107 are used for installing a detection cell, and the detection cell is an optical detection cell and/or an impedance detection cell. The application provides a kit 20 is degradable plastic kit 20, sets up through the subregion and can make things convenient for the motion that moves the liquid device and carry out shorter route when automated inspection.

In a twenty-ninth embodiment, please refer to fig. 15 and 20, an embodiment of the present application provides a reagent kit 20, the reagent kit 20 includes a kit body 100, the reagent kit 20 is used for detecting a sample, and the kit body 100 is provided with a supporting portion so that the reagent kit 20 can be stably supported on a supporting surface.

In one embodiment, the box 100 includes two supporting vertical plates (not shown) as supporting parts. The support vertical plate can be provided with a mark, which is a bar code, a two-dimensional code or an identification chip to record the relevant parameters of the reagent kit 20.

The case 100 is provided with at least one support platform or support surface near the center of gravity of the reagent cartridge 20, and the support platform or support surface serves as a support.

In one embodiment, the case 100 is provided with three supporting protrusions, which form a triangle, and the projection of the center of gravity of the reagent cartridge 20 falls within the area formed by the triangle.

In one embodiment, the cartridge 100 includes at least one cell body, the bottom of which may serve as a support portion.

Optionally, the supporting vertical plate or the supporting platform or the supporting surface or the supporting protrusion and the bottom of the pool body are used together as a supporting part.

The cell body comprises a containing cavity and a skirt 124 (refer to fig. 6, a structure below the bottom 123 of the front cell 120) extending downwards from the containing cavity, the skirt 124 is used as a supporting part, and the containing cavity can contain reagents, samples or accessories (201-204).

The present application provides a cartridge 100 that is a degradable plastic cartridge. The reagent cartridge 20 is provided with an impedance detection cell and/or an optical detection cell.

The reagent kit 20 provided by the embodiment is provided with more cell bodies, and may pass through the bottom of the cell body and/or an additionally arranged supporting part to form a stable support, so as to prevent the reagent kit 20 from easily toppling over.

A thirtieth embodiment, please refer to fig. 15 and 20 together, the embodiment of the present application provides a reagent kit 20, the reagent kit 20 includes a box body 100, the box body 100 includes a plurality of cell bodies, each cell body includes a receiving cavity and a supporting portion connected to the receiving cavity, the supporting portion is a solid structure or a hollow structure, the solid or hollow supporting portion can increase the relative height of the bottom of the receiving cavity, both reagent suction can be facilitated, the corresponding pipetting device can not be inserted too deep, further the pre-storage amount of the reagent can be saved, if the supporting portion is a solid structure, the gravity center of the box body 100 can be moved downward, and the whole is more stable; when the hollow structure is hollow, plastic materials of the box body 100 can be saved.

The height of the supporting part is greater than or equal to the wall thickness of the accommodating cavity, the bottom of the accommodating cavity can be a conical bottom, specifically can be a conical arc bottom, a triangular pyramid or a polygonal pyramid bottom, and the conical bottom extends into a hollow area at the bottom of the supporting part; or the bottom of the accommodating cavity is a plane bottom, and the accommodating cavity is connected with the supporting part through the plane bottom.

When the supporting part is of a hollow structure, the bottom of the supporting part is provided with an opening end or a closed end.

The accommodating cavity and the supporting part are integrally formed or detachably connected.

When the supporting part is a hollow structure, the cross section of the hollow structure is square, round, polygonal or special-shaped, and the height of the supporting part is 0.1-0.8 times of the height of the tank body.

The cell body may be any cell body such as a reagent cell, a detection cell, a sample dilution cell 112, or an attachment placement cell (101-104).

The detection cell is used for impedance detection or optical detection.

The embodiment of the present application further provides a sample detection apparatus, which includes the aforementioned reagent kit 20 and a detection seat 300 matched with the reagent kit 20, wherein the detection seat 300 is used for sample analysis and detection, the detection seat 300 is provided with a power supply assembly electrically connected to the electrode 121 of the front cell 120 and the electrode 165 of the rear cell, and the detection seat 300 can be further provided with optical detection assemblies located at two opposite sides of the optical detection cell (for example, the first cell body 207).

The reagent box 20 that this embodiment provided can make things convenient for the liquid-transfering device to move the liquid through setting up the bottom of the pool of co-altitude not, and the liquid-transfering device can not insert too deeply, and then can practice thrift the volume of prestoring of reagent.

In a thirty-first embodiment, referring to fig. 15 and 20, a reagent cartridge 20 is provided in the embodiment of the present application, where the reagent cartridge 20 includes a cartridge body 100, the cartridge body 100 is provided with accessory placing areas (101-104), and the accessory placing areas are used for placing accessories (201-204).

The lower side of the accessory placing area is provided with a liquid leakage prevention structure which can be a bottom tank. The liquid leakage preventing structure is integrally formed with or detachably connected to the case 100. The accessory placing area is provided with placing holes, and accessories (201-204) are placed in the placing holes.

The aperture of the placing hole is smaller than or equal to the maximum radial dimension of the accessories (201-204), so that the accessories (201-204) are prevented from being completely sunk into the placing hole, and the bottom of the accessories (201-204) is prevented from being in contact deformation with the bottom of the tank body when the accessories (201-204) are inserted.

The box body 100 is provided with a containing cavity corresponding to the placing hole, and accessories (201-204) are contained in the containing cavity. The bottom of the accommodating cavity is provided with a sealing structure.

The receiving cavity is integrally formed with the box 100 or detachably connected thereto.

The depth of the inner cavity of the accommodating cavity is larger than or equal to the length of the accessories (201-204) extending into the accommodating cavity, so that the accessories (201-204) are prevented from excessively protruding out of the surface of the box body 100 when being arranged in the accommodating cavity, and meanwhile, the bottom of the accessories (201-204) is prevented from being in contact with the bottom of the pool body to deform when the accessories (201-204) are inserted.

The length of the accessories (201-204) extending into the accommodating cavity is 0.4-1 times of the length of the accessories.

The embodiment of the present application further provides a sample detection device, which includes the aforementioned reagent kit 20 and a detection seat 300 matched with the reagent kit 20, wherein the detection seat 300 is used for sample analysis and detection.

When accessories are removed, the kit 20 provided by the embodiment can prevent residual liquid carried on the surfaces of the accessories from leaking by receiving the accessories through the bottom pool.

In a thirty-second embodiment, referring to fig. 15 and 20, an embodiment of the present application provides a detection cup assembly, which includes a first tank body 207 and a second tank body 208 connected to the first tank body 207.

The first tank body 207 and the second tank body 208 are connected by a connecting piece 2071, and the connecting piece 2071 is integrally formed with or detachably connected with at least one of the first tank body 207 and the second tank body 208.

The connecting member 2071 is provided with at least one cup holder 2072, and the cup holder 2072 is detachably connected with the first tank body 207 or the second tank body 208.

In an embodiment, the connecting member 2071 is provided with a cup holder 2072 and the connecting member is integrally connected to the first tank 207, and the cup holder 2072 is provided with a placement hole having a shape the same as or different from the cross-sectional shape of the first tank 207.

In an embodiment, the connecting member 2071 is provided with two cup holders 2072, and the two cup holders 2072 are respectively provided with a first placing hole and a second placing hole, and the first placing hole and the second placing hole have the same or different shapes.

The first cell body 207 is a detection cup, the second cell body 208 is a reagent cup, and during detection, a reagent in the second cell body 208 is added into the first cell body 207 to prepare a sample to be detected, and then detection is performed.

The first cell body 207 is provided with an optical detection window for optical detection, such as optical detection by transmitted light or optical detection by scattered light.

The cross-sectional shapes of the first tank body 207 and the second tank body 208 are the same or different; or the heights of the first tank body 207 and the second tank body 208 are the same or different, and a fool-proof effect can be achieved through differential arrangement, so that the assembly and the identification are convenient.

The embodiment of the present application further provides a kit 20, where the kit 20 includes a box body 100 and the aforementioned detection cup assembly, the box body 100 is provided with an installation portion, the detection cup assembly is provided with a matching portion, and the installation portion is connected with the matching portion.

The matching part can be a convex part or a concave part arranged on the side surface of the detection cup component, and the convex part or the concave part is matched and connected with the mounting part; or the matching part is a positioning flange arranged on the detection cup assembly, and the positioning flange is matched and connected with the mounting part, for example, a flange structure is adopted to be inserted into the Kong Guazhuang on the box body 100.

The detection cup subassembly that this embodiment provided novel structure, specific detection project can set corresponding demand reagent on saucer 2072, improvement detection efficiency that can be very big, and detection project can the nimble selection.

In a thirty-third embodiment, referring to fig. 15 to 20, a reagent cartridge 20 is provided in the present embodiment, and the reagent cartridge 20 includes a cartridge body 100 and a mounting seat 160.

Cartridge 100 comprises a forebay 120, forebay 120 being equipped with electrodes 121 of forebay 120; the mounting base 160 is connected to the case 100, the mounting base 160 is mounted with a rear cell electrode 165, and the front cell 120 electrode 121 and the rear cell electrode 165 are spaced apart.

In the embodiment of the application, the axis of the electrode 121 of the front cell 120 and the axis of the electrode 165 of the rear cell are substantially in the same straight line, experiments prove that the detection precision is relatively high when the axis of the electrode 121 of the front cell 120 and the axis of the electrode 165 of the rear cell are coaxial, the electrode 121 of the front cell 120 and the electrode 165 of the rear cell are both columnar electrodes, and the manufacturing process and the assembly process are both simple and convenient.

The assembly seat 160 is provided with an axial drainage cavity 167, the axial drainage cavity 167 is communicated with the front cell 120 through the microporous sheet 170, and the electrode 121 of the front cell 120 and the electrode 165 of the rear cell are positioned at two sides of the microporous sheet 170.

The microporous sheet 170 includes a sheet body 171, the sheet body 171 is provided with micropores 172 allowing cells to pass therethrough one by one, the microporous sheet 170 is mounted on the mounting seat 160 or the cartridge 100, or the microporous sheet 170 and the mounting seat 160 or the cartridge 100 are integrally formed.

The axis of the electrode 121 of the front cell 120, the axis of the electrode 165 of the rear cell and the axis of the microporous sheet 170 are substantially collinear.

Electrode 121 of forebay 120 protrudes, is flush with, or is recessed in the inner wall of forebay 120; the rear cell electrode 165 projects, is flush with, or is recessed into the bottom wall of the axial drainage lumen 167. The two ends of the front cell 120 electrode 121 and the rear cell electrode 165 are not particularly limited, the front cell 120 electrode 121 and the rear cell electrode 165 are in contact with the liquid to be detected during detection, and the outer ends of the front cell 120 electrode 121 and the rear cell electrode 165 are used for externally connecting to an electric power supply component.

The cartridge 100 is provided with a mounting cavity 130, the mounting seat 160 includes a mounting cylinder 168, and the mounting cavity 130 is coaxially connected with the mounting cylinder 168.

The mounting sleeve 168 is snap fit, threaded, interference fit, laser welded, or adhesively engaged with the mounting cavity 130.

The front cell 120 electrode 121 is disposed on the case 100 and protrudes, is flush with, or is recessed on the outer side of the case 100, and the rear cell electrode 165 is disposed on the mounting seat 160 and protrudes, is flush with, or is recessed on the outer side of the mounting seat 160.

In a thirty-fourth embodiment, referring to fig. 15 and 20, the reagent cartridge 20 according to the embodiment of the present disclosure includes a cartridge body 100 and a holding portion 150, wherein the holding portion 150 is disposed on the cartridge body 100.

The grip 150 may be disposed near the center of the side of the case 100 or near the center of the upper surface of the case 100.

The number of the holding portions 150 is two, and the two holding portions 150 are respectively disposed at two opposite sides of the box body 100.

The grip part 150 protrudes from the upper surface or the side surface of the case 100. The outer surface of the grip part 150 is provided with a catching protrusion 151 or a catching recess.

The grip portion 150 is provided with a slip-preventing portion 152 at the tip or outside thereof.

The holding portion 150 is an elastic member, a recess 154 is disposed on a side surface of the box 100, and the holding portion 150 is connected to the recess 154 in a matching manner.

The grip portion 150 is integrally or detachably connected with the recess portion 154.

The holding part 150 is slidably connected with the recess 154, so that the holding part 150 and the box 100 move relatively, the holding part 150 can extend and retract relative to the box 100, and the storage box is convenient, does not occupy space and can be drawn out when needed.

The embodiment of the present application further provides a sample detection device, which includes the aforementioned reagent kit 20 and a detection seat 300 matched with the reagent kit 20, wherein the holding portion 150 is connected with the detection seat 300 in a matching manner.

In a thirty-fifth embodiment, referring to fig. 15 and 20, a reagent cartridge 20 is provided according to an embodiment of the present application, where the reagent cartridge 20 includes a cartridge body 100 and a blocking portion 153.

The cartridge 100 includes at least one well site (e.g., sample receiving well 105); the well is used to hold a test tube (e.g., sample tube 205).

The blocking portion 153 is provided at one side of the well site for hooking a test tube cap (not shown) of the test tube to prevent the opened test tube cap from being restored to cover the open end of the test tube.

In an embodiment, the dam 153 may be disposed to protrude, be flush with, or be recessed in the upper surface or the side surface of the case 100.

In one embodiment, the blocking portion 153 includes a connecting member connected to the case 100 and a stopping portion extending from the connecting member. The end of the blocking portion is provided with a back hook portion extending toward the case 100.

In another embodiment, the side surface of the case 100 is protruded outward to form a dam 153; or the blocking part 153 is a groove formed on the upper surface of the case 100; or the upper surface of the box body 100 is provided with a recess, the inner wall of the recess forms a blocking part 153, a test tube cap of a test tube can be inserted into the recess after being opened, and the size of the recess can correspond to that of the test tube cap to realize relatively tight fit.

The blocking portion 153 is integrally formed with or detachably connected to the case 100. Specifically, the dam 153 is snap-fit, plug-in, threaded, or removably connected to the cartridge 100 via screws/pins.

In other embodiments, the dam 153 is also slidably coupled to the cartridge 100.

In the embodiment, the well (e.g., sample receiving well 105) is disposed near the edge of the cassette 100, and the blocking portion 153 is disposed at the edge of the cassette 100.

The above description is only an embodiment of the present application, and is not intended to limit the scope of the present application, and all equivalent structures or equivalent processes performed by the present application and the contents of the attached drawings, which are directly or indirectly applied to other related technical fields, are also included in the scope of the present application.

Claims

1. A method of using a POCT blood cell analyzer, comprising:

receiving a starting trigger signal;

performing hardware self-check;

initializing the whole machine;

receiving the kit loading;

and carrying out automatic sample pretreatment on the sample on the kit and carrying out sample detection after the automatic sample pretreatment is finished.

2. Use according to claim 1, characterized in that: the hardware self-checking comprises the following steps:

judging whether the pipettor is loaded with an installation head;

if yes, the pipettor carries out installation head unloading, and if not, the complete machine initialization is carried out;

the pipette, after mounting head unloading, comprises:

taking the detection seat out of the bin for taking out the kit;

and the detection seat returns to the bin and then the whole machine initialization step is carried out.

3. The use method according to claim 1, wherein the step of determining whether the pipette is loaded with the mounting head further comprises:

and judging whether the detection seat is internally provided with a kit or not.

4. The use method according to claim 2, wherein after the step of performing the initialization of the whole machine, the method further comprises:

performing pressure detection, and judging whether the pressure in the gas circuit pipeline reaches a threshold range;

performing signal detection, including judging a detection signal after the electromagnetic shielding assembly is matched so as to judge the electromagnetic shielding effect and/or judge whether current and voltage signals are within a threshold range;

entering a standby state.

5. The use of claim 4, wherein the step of entering the standby state is followed by:

receiving a shutdown trigger signal;

judging whether the kit exists or not;

if the kit is judged to be the one with the condition of the door;

if the door is judged to be in an open state, prompting to take out the kit and returning to the step of judging whether the kit exists or not;

if the bin gate is judged to be in a closed state, opening the bin gate, prompting to take out the reagent kit, and returning to the step of judging whether the reagent kit exists or not;

if the kit is judged to be absent, further judging the door state;

if the bin gate is judged to be in an open state, closing the bin gate and prompting to close the power supply;

and if the bin gate is judged to be in a closed state, prompting to close the power supply.

6. The use of claim 4, wherein the step of entering the standby state is followed by:

receiving a sample ID setting and a measurement mode setting;

receiving a starting test trigger signal;

checking whether the information to be measured is matched with the measurement mode, and if not, re-receiving the sample ID setting and the measurement mode setting;

if the detection result is matched, the bin door is opened, and the detection seat is taken out of the bin to receive the reagent kit to be loaded.

7. The use method according to claim 6, further comprising:

detecting whether the kit exists or not;

if not, prompting to put in the kit;

if yes, the bin gate is automatically closed, and an automatic liquid preparation test is carried out.

8. The method of use of claim 7, further comprising:

displaying a test result;

and prompting the test to end.

9. The method of use of claim 8, further comprising:

the door is opened automatically and the kit is taken out.

10. The method of use of claim 9, further comprising:

acquiring a signal that the kit is taken out;

the bin gate is automatically closed.

11. A POCT blood cell analyzer, comprising:

a housing;

the detection seat can extend into or out of the shell, is used for receiving the reagent box to be loaded, and reciprocates between the reagent box loading station and the sample detection station;

and the liquid transfer device is arranged in the shell, is positioned above the detection seat and is used for carrying out automatic sample pretreatment on the kit.

12. The POCT hematology analyzer of claim 11, wherein the reagent cartridge comprises a front cell and a back cell that are connected by a microwell, the POCT hematology analyzer further comprising a pressure building system disposed within the housing for flowing a fluid to be tested from the front cell to the back cell for performing an impedance detection assay.

13. The POCT hematology analyzer of claim 12, further comprising a metal shielding cover for covering the detection seat, wherein the reagent kit comprises a pressure action cavity communicated with the rear pool, the metal shielding cover is provided with a pneumatic connecting device connected with the pressure building system, and the pneumatic connecting device is in sealed butt joint with the pressure action cavity when the metal shielding cover is covered with the detection seat.

14. The POCT blood cell analyzer of claim 12, wherein the pipette includes a detection member, the pipette includes a gas-guiding tube and an outer sleeve sleeved on the gas-guiding tube, the gas-guiding tube is used for loading a mounting head mounted on the reagent kit, when the gas-guiding tube loads the mounting head, the gas-guiding tube is inserted into the mounting head, so that the mounting head pushes the outer sleeve to generate a position change along the axial direction of the gas-guiding tube, and the detection member is used for detecting the position of the outer sleeve to determine whether the mounting head is sleeved on the gas-guiding tube.

15. The POCT cytometer of claim 12, wherein the pipettor further comprises a tip retracting mechanism for stopping the ascent of the overtube when the airway tube ascends so that the overtube unloads the mounting head.

16. The POCT hematology analyzer of claim 15, wherein the reversing mechanism is an electromagnet, a motor, or a U-shaped orifice baffle that is axially or radially disposed relative to the airway tube.

17. The POCT blood cell analyzer of claim 12, wherein the pressure buildup system comprises a pressure chamber, a first injector and a second injector, the first injector and the second injector are linked, the first injector is used for establishing positive pressure or negative pressure for the pressure chamber, and the second injector is communicated with the pipette and is used for sucking reagent and/or sample by using the inner cavity of the mounting head as a transfer position in liquid when the pipette is sleeved with the mounting head.

18. The POCT cytometer of claim 17, wherein the POCT cytometer comprises a first conduit connected between the second syringe and the pipette, and a second conduit for connecting the syringe and the pressure chamber, wherein the first conduit has an inner diameter smaller than an inner diameter of the second conduit.

19. The POCT cytometer of claim 18, wherein the first conduit is provided with an anti-folding member around its periphery, wherein the anti-folding member is a sleeve, a spring or a strap.

20. The POCT blood cell analyzer is characterized by comprising a detection seat, a liquid transfer device arranged above the detection seat and a pressure building system, wherein the detection seat is used for receiving a kit to be loaded, the kit is provided with a front pool and a rear pool which are communicated through micropores and provided with a reagent, a sample and an installing head, and the pressure building system comprises:

a pressure chamber;

the air pressure connecting device is connected with the pressure chamber and is used for applying positive pressure or negative pressure to the front pool or the rear pool to enable liquid in the front pool to flow to the rear pool;

the first injector is communicated with the pressure chamber/the pipettor and is used for establishing positive pressure or negative pressure for the pressure chamber and assisting the pipettor to move liquid or bubble and mix liquid in the forebay;

a second syringe in communication with the pipettor for pipetting;

a drive member for simultaneously driving the first and second syringes;

a first solenoid valve selectively communicating the first injector with the pressure chamber and the second injector;

a second solenoid valve selectively communicating the first solenoid valve with the pressure chamber and the outside atmosphere;

a third solenoid valve selectively communicating the pneumatic connection with the pressure chamber.