CN115247051B - Microbial oil liquid lubricant for drilling fluid and preparation method and application thereof - Google Patents
Microbial oil liquid lubricant for drilling fluid and preparation method and application thereof Download PDFInfo
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- CN115247051B CN115247051B CN202110455226.0A CN202110455226A CN115247051B CN 115247051 B CN115247051 B CN 115247051B CN 202110455226 A CN202110455226 A CN 202110455226A CN 115247051 B CN115247051 B CN 115247051B
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- 230000000813 microbial effect Effects 0.000 title claims abstract description 204
- 239000003921 oil Substances 0.000 title claims abstract description 170
- 238000005553 drilling Methods 0.000 title claims abstract description 108
- 239000010687 lubricating oil Substances 0.000 title claims abstract description 101
- 239000012530 fluid Substances 0.000 title claims abstract description 100
- 238000002360 preparation method Methods 0.000 title claims abstract description 23
- 238000002156 mixing Methods 0.000 claims abstract description 23
- 239000002994 raw material Substances 0.000 claims abstract description 19
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 14
- 238000012258 culturing Methods 0.000 claims abstract description 12
- 241000223253 Rhodotorula glutinis Species 0.000 claims abstract description 10
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- 235000019198 oils Nutrition 0.000 claims description 159
- 238000000855 fermentation Methods 0.000 claims description 42
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- 239000007788 liquid Substances 0.000 claims description 16
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- RYYKJJJTJZKILX-UHFFFAOYSA-M sodium octadecanoate Chemical compound [Na+].CCCCCCCCCCCCCCCCCC([O-])=O RYYKJJJTJZKILX-UHFFFAOYSA-M 0.000 claims description 13
- -1 fatty acid ester Chemical class 0.000 claims description 12
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- 239000004519 grease Substances 0.000 claims description 7
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- HJVAFZMYQQSPHF-UHFFFAOYSA-N 2-[bis(2-hydroxyethyl)amino]ethanol;boric acid Chemical compound OB(O)O.OCCN(CCO)CCO HJVAFZMYQQSPHF-UHFFFAOYSA-N 0.000 claims description 4
- NWGKJDSIEKMTRX-AAZCQSIUSA-N Sorbitan monooleate Chemical compound CCCCCCCC\C=C/CCCCCCCC(=O)OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O NWGKJDSIEKMTRX-AAZCQSIUSA-N 0.000 claims description 4
- AGXUVMPSUKZYDT-UHFFFAOYSA-L barium(2+);octadecanoate Chemical compound [Ba+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O AGXUVMPSUKZYDT-UHFFFAOYSA-L 0.000 claims description 4
- 239000008116 calcium stearate Substances 0.000 claims description 4
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- GVGUFUZHNYFZLC-UHFFFAOYSA-N dodecyl benzenesulfonate;sodium Chemical compound [Na].CCCCCCCCCCCCOS(=O)(=O)C1=CC=CC=C1 GVGUFUZHNYFZLC-UHFFFAOYSA-N 0.000 claims description 4
- 235000019359 magnesium stearate Nutrition 0.000 claims description 4
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- XOOUIPVCVHRTMJ-UHFFFAOYSA-L zinc stearate Chemical compound [Zn+2].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O XOOUIPVCVHRTMJ-UHFFFAOYSA-L 0.000 claims description 4
- 238000004519 manufacturing process Methods 0.000 claims description 2
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical group CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 claims description 2
- 241000222175 Diutina rugosa Species 0.000 claims 1
- 239000013505 freshwater Substances 0.000 abstract description 25
- 239000000314 lubricant Substances 0.000 abstract description 14
- 230000001050 lubricating effect Effects 0.000 abstract description 14
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- 241000222120 Candida <Saccharomycetales> Species 0.000 abstract description 6
- 238000006065 biodegradation reaction Methods 0.000 abstract description 5
- 231100000053 low toxicity Toxicity 0.000 abstract description 5
- 231100000956 nontoxicity Toxicity 0.000 abstract description 5
- 239000013535 sea water Substances 0.000 abstract description 3
- 239000002002 slurry Substances 0.000 description 45
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 24
- 230000000052 comparative effect Effects 0.000 description 24
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- 238000005461 lubrication Methods 0.000 description 21
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- 238000012360 testing method Methods 0.000 description 15
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- 230000032683 aging Effects 0.000 description 13
- 239000001963 growth medium Substances 0.000 description 13
- 239000002609 medium Substances 0.000 description 11
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- 238000011156 evaluation Methods 0.000 description 10
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 9
- 230000018109 developmental process Effects 0.000 description 8
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- 229920001817 Agar Polymers 0.000 description 6
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- 230000001276 controlling effect Effects 0.000 description 6
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- HPALAKNZSZLMCH-UHFFFAOYSA-M sodium;chloride;hydrate Chemical compound O.[Na+].[Cl-] HPALAKNZSZLMCH-UHFFFAOYSA-M 0.000 description 6
- 239000007858 starting material Substances 0.000 description 5
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- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 241000223233 Cutaneotrichosporon cutaneum Species 0.000 description 2
- 241001149691 Lipomyces starkeyi Species 0.000 description 2
- 241000529878 Lipomyces tetrasporus Species 0.000 description 2
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 2
- DKXNBNKWCZZMJT-UHFFFAOYSA-N O4-alpha-D-Mannopyranosyl-D-mannose Natural products O=CC(O)C(O)C(C(O)CO)OC1OC(CO)C(O)C(O)C1O DKXNBNKWCZZMJT-UHFFFAOYSA-N 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000002026 chloroform extract Substances 0.000 description 2
- 230000007797 corrosion Effects 0.000 description 2
- 238000005260 corrosion Methods 0.000 description 2
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- 239000002283 diesel fuel Substances 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
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- 229960002160 maltose Drugs 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 239000002184 metal Substances 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 239000000401 methanolic extract Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 229940049964 oleate Drugs 0.000 description 2
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
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- 235000015112 vegetable and seed oil Nutrition 0.000 description 2
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- 239000002699 waste material Substances 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 102000057297 Pepsin A Human genes 0.000 description 1
- 108090000284 Pepsin A Proteins 0.000 description 1
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 1
- 241000258161 Stichopus Species 0.000 description 1
- 241000223230 Trichosporon Species 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 150000004945 aromatic hydrocarbons Chemical class 0.000 description 1
- 239000000440 bentonite Substances 0.000 description 1
- 229940092782 bentonite Drugs 0.000 description 1
- 229910000278 bentonite Inorganic materials 0.000 description 1
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- ONCZQWJXONKSMM-UHFFFAOYSA-N dialuminum;disodium;oxygen(2-);silicon(4+);hydrate Chemical compound O.[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[O-2].[Na+].[Na+].[Al+3].[Al+3].[Si+4].[Si+4].[Si+4].[Si+4] ONCZQWJXONKSMM-UHFFFAOYSA-N 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000003925 fat Substances 0.000 description 1
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- 239000007789 gas Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000003301 hydrolyzing effect Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000004020 luminiscence type Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 150000002739 metals Chemical class 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000003345 natural gas Substances 0.000 description 1
- 235000014593 oils and fats Nutrition 0.000 description 1
- 229940111202 pepsin Drugs 0.000 description 1
- 239000003209 petroleum derivative Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K8/00—Compositions for drilling of boreholes or wells; Compositions for treating boreholes or wells, e.g. for completion or for remedial operations
- C09K8/02—Well-drilling compositions
- C09K8/03—Specific additives for general use in well-drilling compositions
- C09K8/035—Organic additives
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2208/00—Aspects relating to compositions of drilling or well treatment fluids
- C09K2208/34—Lubricant additives
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Materials Engineering (AREA)
- Organic Chemistry (AREA)
- Lubricants (AREA)
Abstract
The invention relates to the technical field of oil and gas well exploitation, and provides a microbial oil liquid lubricant for drilling fluid, a preparation method and application thereof, wherein the main raw material of the lubricant is microbial oil; the microbial oil is prepared by respectively culturing 4 oil-producing strains, and mixing the 4 oil-producing strains, wherein the 4 oil-producing strains are respectively Saccharomyces cerevisiae, candida Pityrosporum, rhodotorula glutinis and ascomycete oleaginous yeast. The microbial oil liquid lubricant for the drilling fluid provided by the embodiment of the invention has the advantages of good lubricating effect, no fluorescence, no toxicity or low toxicity, environment friendliness, easiness in biodegradation and good compatibility with a drilling fluid system. The microbial oil liquid lubricant for the drilling fluid provided by the embodiment of the invention is suitable for well type drilling fluids such as vertical wells, inclined wells, horizontal wells and the like, and fresh water and sea water systems.
Description
Technical Field
The invention relates to the technical field of oil and gas well exploitation, in particular to a microbial oil liquid lubricant for drilling fluid and a preparation method and application thereof.
Background
The lubricant for drilling fluid in the petroleum and natural gas drilling process is an important drilling fluid additive, and has the functions of improving the lubricating performance of the drilling fluid, reducing the friction between a drilling tool, a sleeve and a well wall, reducing the rotation torque of a drill string in the drilling process and the resistance generated during tripping, and minimizing the occurrence rate of stuck drilling accidents.
The liquid lubricants for the drilling fluid are various in types, and the main raw materials comprise crude oil, diesel oil, white oil, vegetable oil and the like, but are mostly non-renewable resources, contain aromatic hydrocarbons, are difficult to degrade, and have the problems of environmental pollution and the like in the production and use processes.
Today, economic development of all countries in the world is under double pressure of energy supply safety and ecological environment protection, and in order to realize sustainable coordinated development of energy, environment and economy, all countries are devoting to development of renewable energy. The oil-producing microorganism can efficiently utilize carbohydrate obtained by hydrolyzing cellulose and hemicellulose to produce grease, and the grease is one of basic substances for constructing and maintaining life movement and is also an important industrial raw material, so that the microbial oil-producing technology has great development prospect in the aspect of relieving global energy crisis.
However, how to develop a lubricant for drilling fluids that is environmentally friendly and has good performance is still one of the significant research subjects.
Disclosure of Invention
Aiming at the dual pressures of energy supply safety and ecological environment protection facing the economic development of various countries in the world at present, in order to realize sustainable and coordinated development of energy, environment and economy and research and development of lubricants for drilling fluids, the application provides a microbial oil liquid lubricant for the drilling fluids, and a preparation method and application thereof.
The invention provides a microbial oil liquid lubricant for drilling fluid, which is prepared from the following main raw materials of microbial oil; the microbial oil is prepared by respectively culturing 4 oil-producing strains, and mixing the 4 oil-producing strains, wherein the 4 oil-producing strains are respectively Saccharomyces cerevisiae, candida Pityrosporum, rhodotorula glutinis and ascomycete oleaginous yeast.
In a preferred embodiment of the present invention, the microbial oil is prepared according to the following steps:
culturing the 4 oleaginous strains respectively to obtain 4 corresponding microbial fermentation broths respectively; mixing the 4 microbial fermentation liquids according to the volume ratio to obtain a microbial fermentation mixed liquid, and centrifugally separating the microbial fermentation mixed liquid to obtain microbial thalli; finally, extracting the microbial thalli by an acid heating method to obtain the microbial oil.
In the preferred embodiment of the invention, the temperature for respectively culturing the 4 oleaginous strains is 24-30 ℃, and the culture time is more than 24 hours.
In a preferred embodiment of the present invention, the starting materials of the microbial oil liquid lubricant for drilling fluid include the microbial oil and a stabilizer.
In a preferred embodiment of the present invention, the stabilizer is selected from the group consisting of stearates, in particular one or more of calcium stearate, zinc stearate, sodium stearate, magnesium stearate and barium stearate.
In a preferred embodiment of the present invention, the starting material of the microbial oil liquid lubricant for drilling fluid further comprises an emulsifier.
Further preferably, the emulsifier is selected from one or more of sodium dodecyl benzene sulfonate, fatty acid methyl ester sulfonate, succinic acid ester sulfonate, span 80 and tween 85.
In a preferred embodiment of the present invention, the starting material of the microbial oil liquid lubricant for drilling fluids further comprises an antiwear agent.
Further preferably, the antiwear agent is one or more selected from vulcanized lard, vulcanized cottonseed oil, vulcanized fatty acid ester and triethanolamine borate.
In a more preferred embodiment of the invention, the microbial oil liquid lubricant for drilling fluid consists of the following raw materials in parts by weight:
70-90 parts of microbial oil; 1-3 parts of antiwear agent; 3-7 parts of stabilizer; 3-5 parts of emulsifying agent.
The embodiment of the invention provides a preparation method of the microbial oil liquid lubricant for drilling fluid, which comprises the following steps: mixing the microbial oil and other raw materials according to the weight ratio for more than 5 hours at the temperature of 50-80 ℃ and the stirring speed of 300-600 rpm to obtain the microbial oil liquid lubricant for drilling fluid.
In addition, the embodiment of the invention provides application of the microbial oil liquid lubricant for drilling fluid in preparation of drilling fluid.
Compared with the prior art, the microbial oil liquid lubricant for the drilling fluid is mainly prepared by respectively culturing and mixing 4 different oil-producing strains of the candida stichopus, the candida pinosus, the rhodotorula glutinis and the ascomycete oleaginous. The embodiment of the invention successfully prepares the microbial oil liquid lubricant for drilling fluid by utilizing microbial oil, and the main raw material system microbial oil is produced to replace the main raw materials of the current main-stream lubricant, such as crude oil, diesel oil, white oil, vegetable oil and the like; the lubricant has the advantages of good lubricating effect, easy biodegradation, environmental protection, good compatibility with drilling fluid systems, and the like, and has great development prospect.
Furthermore, the microbial oil adopted by the invention has short fermentation period, is not influenced by site, season, climate change and the like, can be continuously produced in four seasons, has rich resources of oleaginous microbial strains, has broad spectrum of carbon sources, and can utilize and convert various agricultural and forestry waste lignocellulose raw materials.
After the microbial oil liquid lubricant for the drilling fluid is added into the drilling fluid, the fluid loss is basically unchanged, and the microbial oil liquid lubricant is favorable for stabilizing the well wall. The microbial oil liquid lubricant for the drilling fluid provided by the embodiment of the invention has good stability in water-based drilling fluid, and can obviously reduce the extreme pressure lubrication coefficient. The microbial oil liquid lubricant for the drilling fluid provided by the embodiment of the invention is suitable for well type drilling fluids such as vertical wells, inclined wells, horizontal wells and the like, and fresh water and sea water systems.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
The object of the present invention is achieved by the following technical solutions, aiming at the above problems in the prior art:
the invention provides a microbial oil liquid lubricant for drilling fluid, which is prepared from the following main raw materials of microbial oil; the microbial oil is prepared by respectively culturing 4 oil-producing strains, and mixing the 4 oil-producing strains, wherein the 4 oil-producing strains are respectively Saccharomyces cerevisiae, candida Pityrosporum, rhodotorula glutinis and ascomycete oleaginous yeast.
The microbial oil liquid lubricant for the drilling fluid provided by the embodiment of the invention has the advantages of good lubricating effect, no fluorescence, no toxicity or low toxicity, environmental friendliness, easiness in biodegradation and good compatibility with a drilling fluid system, and is suitable for well types such as vertical wells, inclined wells, horizontal wells and the like.
The preparation method of the microbial oil in the specific embodiment of the invention comprises the following steps:
(1) Preparing a microbial fermentation mixed solution: respectively culturing 4 different oleaginous strains in 4 culture mediums to respectively obtain 4 corresponding microbial fermentation broths; then taking the 4 microbial fermentation broths according to the volume ratio, and uniformly mixing to obtain a microbial fermentation mixed liquor;
(2) And (3) centrifugal separation: centrifugally separating the microbial fermentation mixed liquor obtained in the step (1) to obtain mixed microbial thalli;
(3) Acid heating extraction: adding acid into the microbial cells obtained in the step (2), uniformly mixing, heating and cooling; and adding the chloroform/methanol mixed extract, mixing, centrifuging to recover chloroform layer, and volatilizing to remove chloroform to obtain microbial oil.
The oleaginous strain in the above (1) step comprises: oleaginous yeast of the genus Latin Wen Xueming Lipomyces starkeyi, accession number CICC 1809, accession number CM0077 (main component: 5°Be wort, agar, etc.);
trichosporon Pitussilago (Latin Wen Xueming Trichosporon cutaneum, accession number: GDMCC2.68, accession number: guangdong province microorganism strain collection, medium number: 13 (main ingredient: malt extract, D-maltose, agar, etc.));
rhodotorula glutinis (Latin Wen Xueming Rhodotorula glutinis, accession number: GDMCC 2.28, accession number: guangdong province microorganism strain collection, medium number: 13 (main ingredient: malt extract, D-maltose, agar, etc.);
ascomycete oleaginous yeast (latin Wen Xueming Lipomyces tetrasporus, accession number: ATCC32372, accession number: american type culture collection, medium:culture medium 200: YM agar or YM broth (main component: pepsin digest of animal tissue, glucose, agar, etc.)).
The carbon source of the oleaginous microorganism strain provided by the embodiment of the invention is broad-spectrum, and can be used for utilizing and converting various agricultural and forestry waste lignocellulose raw materials. The invention adopts the 4 microbial agents to culture and mix, so that the produced grease has good lubricity, and the problems of poor adaptability and the like of single-component microbial agents in use can be avoided. If 1 or two mixed bacterial agents of 4 bacterial agents are used, the performance of the product cannot meet the use requirement.
In the specific embodiment of the invention, the 4 culture mediums are respectively a culture medium for obtaining a fermentation broth of the oleaginous yeast of the Sterill, a culture medium for a fermentation broth of the Pityrosporum, a culture medium for a fermentation broth of the Rhodotorula glutinis and a culture medium for a fermentation broth of the ascomycetes oleaginous yeast; the main composition of each medium is as described above.
In the present invention, the 4 oleaginous strains and their culture media are all commercially available products, which are available from their collection. The basic parameters of the corresponding 4 microorganism fermentation broths are as follows:
TABLE 1 basic parameters of 4 microbial fermentation broths according to the examples of the invention
In the invention, the culture temperature and the culture time of the oleaginous strain are critical, the culture temperature influences the regulation of fatty acid components by cells, the proper temperature is beneficial to the propagation and survival rate of thalli, and the culture time influences the total number of thalli, thereby influencing the yield of grease. In the preferred embodiment of the invention, the temperature for respectively culturing the 4 oleaginous strains is 24-30 ℃ and can be 28-30 ℃; the culture time is more than 24 hours, for example, 24-48 hours. The specific operation can be as follows: the inoculation amount of the strain is 1-3%, the temperature is controlled to be 24-30 ℃, and the strain is preferably cultured for 24-48 hours at 200rpm in an oscillating way, so that 4 microbial fermentation broths are obtained.
And according to the volume ratio, 1 part of each of the 4 microorganism fermentation broths is uniformly mixed to obtain a microorganism fermentation mixed liquid, and then the microorganism fermentation mixed liquid is separated to obtain microorganism mixed thalli. In addition, the process for extracting microbial oil by the acid heating method is not particularly limited; examples of the acid heating process are: firstly, adding 6mL of hydrochloric acid (the concentration is 4 mol/L) into each gram of microorganism mixed thallus, shaking and mixing uniformly, standing for 30min, boiling for 3min, rapidly cooling at-20 ℃, and recording the volume as V 0 The method comprises the steps of carrying out a first treatment on the surface of the Then add 2 volumes (2V 0 ) Is a chloroform/methanol extract (chloroform/methanol volume ratio of 1: 1) And (3) after uniformly mixing, centrifuging at 3000rpm for 15min to recover a chloroform layer, adding 20-50 mL of sodium chloride solution (mass fraction is 0.1%), uniformly mixing, centrifuging at 3000rpm for 15min to recover the chloroform layer, and volatilizing to remove chloroform to obtain the microbial oil.
In a specific embodiment of the invention, the main component oleate of the prepared microbial oil comprises the following technical indexes: density of 0.84g/cm 3 ～0.88g/cm 3 Boiling point 168-170 ℃, viscosity 5.1 mPas-5.5 mPas, flash point > 110 ℃, and the like.
In a preferred embodiment of the present invention, the raw materials of the microbial oil liquid lubricant for drilling fluid of the present invention include a stabilizer in addition to the microbial oil, so as to further improve the overall performance of the microbial oil liquid lubricant. The stabilizer is preferably stearate, specifically one or more of calcium stearate, zinc stearate, sodium stearate, magnesium stearate and barium stearate, and more preferably sodium stearate.
In a preferred embodiment of the present invention, the starting material of the microbial oil liquid lubricant for drilling fluid further comprises an emulsifier. The emulsifier can be one or more selected from sodium dodecyl benzene sulfonate, fatty acid methyl ester sulfonate, succinic acid ester sulfonate, span 80 and tween 85.
In a preferred embodiment of the present invention, the starting material of the microbial oil liquid lubricant for drilling fluids further comprises an antiwear agent. More preferably, the antiwear agent is one or more of vulcanized lard, vulcanized cottonseed oil, vulcanized fatty acid ester and triethanolamine borate.
Specifically, the raw materials of the microbial oil liquid lubricant for the drilling fluid comprise microbial oil, a stabilizer, an emulsifier, an antiwear agent and the like. In a preferred embodiment of the invention, the microbial oil liquid lubricant for drilling fluid consists of the following raw materials in parts by weight: 70-90 parts of microbial oil; 1-3 parts of antiwear agent; 3-7 parts of stabilizer; 3-5 parts of emulsifying agent.
In the present invention, the weight part of the microbial oil is preferably 75 to 90 parts, more preferably 77 to 85 parts, and most preferably 82 parts.
In the present invention, the weight part of the antiwear agent is preferably 1.5 to 2.5 parts, more preferably 1.8 to 2.2 parts, and most preferably 2 parts. In the invention, the antiwear agent is preferably one or more of vulcanized lard, vulcanized cottonseed oil, vulcanized fatty acid ester and triethanolamine borate.
In the present invention, the weight part of the stabilizer is preferably 4 to 6 parts, more preferably 4.5 to 5.5 parts, and most preferably 5 parts. For example, the stabilizer includes one or more of calcium stearate, zinc stearate, sodium stearate, magnesium stearate and barium stearate.
In the present invention, the weight part of the emulsifier is preferably 3.5 to 4.5 parts, more preferably 3.8 to 4.2 parts, and most preferably 3.9 parts. For example, the emulsifier comprises one or more of sodium dodecyl benzene sulfonate, fatty acid methyl ester sulfonate, succinic acid ester sulfonate, span 80 and tween 85. The auxiliary components in the embodiment of the invention can better ensure that the lubricant keeps stable state, stable performance and improved lubricity, and are beneficial to application.
The embodiment of the invention provides a preparation method of the microbial oil liquid lubricant for drilling fluid, which comprises the following steps: mixing the microbial oil and other raw materials according to the weight ratio for more than 5 hours at the temperature of 50-80 ℃ and the stirring speed of 300-600 rpm to obtain the microbial oil liquid lubricant for drilling fluid. The specific operation can be as follows: and (2) fully mixing 70-90 parts of microbial oil, 1-3 parts of antiwear agent, 3-7 parts of stabilizer and 3-5 parts of emulsifier for 5-8 hours under the conditions that the temperature is 50-80 ℃ and the stirring speed is 300-600 rpm to obtain the microbial oil liquid lubricant for drilling fluid.
In the preparation method of the microbial oil liquid lubricant for drilling fluid provided by the embodiment of the invention, the types and the amounts of the microbial oil, the antiwear agent, the stabilizer and the emulsifier are the same as those of the microbial oil, the antiwear agent, the stabilizer and the emulsifier in the technical scheme, and are not repeated here.
In the preparation method of the embodiment of the invention, the mixing temperature is preferably 50-70 ℃, more preferably 55-65 ℃ and most preferably 60 ℃; the mixing time is preferably 5 to 7 hours, more preferably 5.5 to 6.5 hours, most preferably 6 hours; the mixing is performed under stirring conditions, preferably at a speed of 350 to 550rpm, more preferably 400 to 500rpm, most preferably 450rpm.
The invention adopts microbial oil to successfully prepare the microbial oil liquid lubricant for drilling fluid; the lubricant has the advantages of good lubricating effect, no fluorescence, no toxicity or low toxicity, environmental protection, easy biodegradation and good compatibility with drilling fluid systems.
The microbial oil liquid lubricant for the drilling fluid provided by the embodiment of the invention has the advantages that the volume addition amount of the microbial oil liquid lubricant for the drilling fluid is generally 2-4% in the applied water-based drilling fluid, the stability of the drilling fluid is good, and the extreme pressure lubrication coefficient can be obviously reduced. Some experimental results show that: the reduction rate of the lubricating coefficient in the fresh water slurry is more than 78%, the reduction rate of the lubricating coefficient in the 4% brine slurry is more than 75%, and the reduction rate of the lubricating coefficient in the saturated brine slurry is more than 60%; when the addition amount of the additive in the fresh water base slurry is 1%, the apparent viscosity change is small, the thickening is avoided, and the foaming rate is less than 4%; the compatibility in the water-based drilling fluid is good, the rheological property of the drilling fluid is not obviously affected, and the water-based drilling fluid plays an important role in reducing the underground complex conditions such as stuck drilling and the like and ensuring safe, high-quality and quick drilling.
After the microbial oil liquid lubricant for the drilling fluid provided by the embodiment of the invention is added into the drilling fluid, the filtration loss is basically unchanged, and the microbial oil liquid lubricant is favorable for stabilizing the well wall.
The microbial oil liquid lubricant for the drilling fluid provided by the embodiment of the invention is nontoxic or low in toxicity, low in fluorescence, and has a fluorescence level of less than 3, no corrosion to metals, no pollution to the environment, biodegradability and no influence on analysis and evaluation of stratum data.
After the microbial oil liquid lubricant for the drilling fluid provided by the embodiment of the invention is added into the drilling fluid, EC of the microbial oil liquid lubricant is prepared 50 The value is more than 110000mg/L, and the environmental protection performance is good.
The microbial oil liquid lubricant for the drilling fluid provided by the embodiment of the invention is suitable for well type drilling fluids such as vertical wells, inclined wells, horizontal wells and the like, and fresh water and sea water systems.
For further understanding of the present application, the microbial oil liquid lubricant for drilling fluids provided herein, and the preparation method and application thereof are specifically described below with reference to examples. The raw materials used in the following examples of the present invention are all commercially available.
Example 1 preparation of microbial fats and oils
(1) Preparation of microbial mixed fermentation liquor
a. Inoculating oleaginous yeast (Latin Wen Xueming Lipomyces starkeyi, accession number: CICC 1809, accession number: china industry microbiological culture Collection center) to a culture medium (culture medium number: CM 0077), controlling the inoculation amount to be 3%, and performing shake culture at 28-30 ℃ and a rotating speed of 200rpm for 24-48 hours to obtain a microbial fermentation broth A;
b. inoculating the candida Pitussilago (Latin Wen Xueming Trichosporon cutaneum, accession number: GDMCC2.68, accession number: guangdong province microorganism strain collection center) into a culture medium (culture medium number: 13), controlling the inoculation amount to be 2%, controlling the culture temperature to be 28-30 ℃, and performing shake culture at a rotating speed of 200rpm for 24-48 hours to obtain a microorganism fermentation liquor B;
c. inoculating rhodotorula glutinis (Latin Wen Xueming Rhodotorula glutinis, accession number: GDMCC 2.28, accession number: guangdong province microorganism strain collection center) to a culture medium (culture medium number: 13), controlling the inoculation amount to be 2%, controlling the culture temperature to be 25-28 ℃, and carrying out shake culture at a rotation speed of 200rpm for 24-48 hours to obtain a microorganism fermentation liquor C;
d. ascomycete oleaginous yeast (latin Wen Xueming Lipomyces tetrasporus, accession number: ATCC32372, accession number: american type culture collection) was inoculated into a medium (medium:culture medium 200: YM agar or YM broth) with an inoculum size of 3%, controlling the culture temperature at 24 ℃ and performing shake culture at 200rpm for 24-48 h to obtain a microbial fermentation broth D;
e. the 4 kinds of microorganism fermentation broths A, B, C, D (basic parameters are shown in the above table 1) obtained in the above were uniformly mixed in 1 part by volume, to obtain microorganism mixed fermentation broths.
(2) Centrifugal separation
Centrifugally separating the microbial mixed fermentation broth obtained in the step (1) at a speed of 3000rpm to obtain microbial mixed thalli;
(3) Extraction of microbial oil by acid heating method
a. Adding 6mL of hydrochloric acid (with the concentration of 4 mol/L) into each gram of microorganism mixed thallus obtained in the step (2), shaking and mixing uniformly, standing for 30min, boiling for 3min, rapidly cooling at-20 ℃, and recording the volume as V at the moment 0 ；
b. Add 2 volumes (2V 0 ) Is a chloroform/methanol extract (chloroform/methanol volume ratio of 1: 1) After uniform mixing, the chloroform layer is recovered by centrifugation at 3000rpm for 15 min;
c. adding 20-50 mL sodium chloride solution (mass fraction 0.1%), mixing, centrifuging at 3000rpm for 15min, recovering chloroform layer, volatilizing to remove chloroform, and obtaining microbial oil.
The main component oleate of the microbial oil prepared comprises the following technical indexes: density of 0.84g/cm 3 ～0.88g/cm 3 The boiling point is 168-170 ℃, the viscosity is 5.1-5.5 mPas, and the flash point is more than 110 ℃.
Example 2 preparation of Lubricants
70 parts by weight of the microbial oil prepared in example 1, 1.5 parts by weight of a sulfurized fatty acid ester (the fatty acid chain length is 16 to 18, the same applies to the following examples), 4 parts by weight of sodium stearate, 3.5 parts by weight of a fatty acid methyl ester sulfonate (the fatty acid chain length is 16 to 18, the same applies to the following examples) were added to a reaction vessel, and mixed for 5 hours at 50℃and 350rpm to obtain a microbial oil liquid lubricant for drilling fluids.
Example 3
77 parts by weight of the microbial oil prepared in example 1, 1.8 parts by weight of a sulfurized fatty acid ester, 4.5 parts by weight of sodium stearate, 3.8 parts by weight of fatty acid methyl ester sulfonate were added to a reaction kettle, and reacted at 55℃and 400rpm for 5.5 hours to obtain a microbial oil liquid lubricant for drilling fluid.
Example 4
82 parts by weight of the microbial oil prepared in example 1, 2 parts by weight of a sulfurized fatty acid ester, 5 parts by weight of sodium stearate, and 3.9 parts by weight of fatty acid methyl ester sulfonate were added to a reaction vessel, and reacted at 60℃and 450rpm for 6 hours to obtain a microbial oil liquid lubricant for drilling fluid.
Example 5
85 parts by weight of the microbial oil prepared in example 1, 2.2 parts by weight of a vulcanized fatty acid ester, 5.5 parts by weight of sodium stearate and 4.2 parts by weight of fatty acid methyl ester sulfonate are added into a reaction kettle and reacted for 6.5 hours at 65 ℃ and 500rpm to obtain the microbial oil liquid lubricant for drilling fluid.
Example 6
90 parts by weight of the microbial oil prepared in example 1, 2.5 parts by weight of a sulfurized fatty acid ester, 6 parts by weight of sodium stearate, and 4.5 parts by weight of fatty acid methyl ester sulfonate were added to a reaction kettle, and reacted at 70℃and 550rpm for 7 hours to obtain a microbial oil liquid lubricant for drilling fluid.
Example 7
80 parts by weight of the microbial oil prepared in example 1, 2 parts by weight of a vulcanized fatty acid ester, 5 parts by weight of sodium stearate and 4.0 parts by weight of fatty acid methyl ester sulfonate are added into a reaction kettle and reacted for 6 hours at 60 ℃ and 450rpm to obtain the microbial oil liquid lubricant for drilling fluid.
Example 8
90 parts by weight of the microbial oil prepared in example 1, 2.5 parts by weight of a sulfurized fatty acid ester, 6 parts by weight of sodium stearate, and 4.5 parts by weight of fatty acid methyl ester sulfonate were added to a reaction kettle, and reacted at 70℃and 550rpm for 7 hours to obtain a microbial oil liquid lubricant for drilling fluid.
Example 9
The procedure of example 4 was followed to prepare a microbial oil liquid lubricant for drilling fluids, except that the vulcanized fatty acid ester was not added during the preparation, and 92 parts by weight of the microbial oil prepared in example 1 was used instead of 82 parts by weight of the microbial oil in example 4.
Example 10
A microbial oil liquid lubricant for drilling fluids was prepared according to the procedure of example 4, except that sodium stearate was not added during the preparation, and 82 parts by weight of the microbial oil of example 4 was replaced with 85 parts by weight of the microbial oil of example 1.
Comparative example 1
A microbial oil liquid lubricant for drilling fluids was prepared as in example 4, except that the microbial oil used was replaced with microbial oil extracted from microbial fermentation broth A by the acid heating method.
Comparative example 2
A microbial oil liquid lubricant for drilling fluids was prepared as in example 4, except that the microbial oil used was replaced with microbial oil extracted from microbial fermentation broth C by the acid heating method.
Comparative example 3
A microbial oil liquid lubricant for drilling fluids was prepared as in example 4, except that the microbial oil used was replaced with: microbial oil extracted from microbial fermentation broth A, B by an acid heating method according to a volume ratio of 1:1.
Comparative example 4
A microbial oil liquid lubricant for drilling fluids was prepared as in example 4, except that the microbial oil used was replaced with: microbial oil extracted from microbial fermentation broth C, D by an acid heating method according to a volume ratio of 1:1.
Comparative example 5
A microbial oil liquid lubricant for drilling fluids was prepared as in example 4, except that the microbial oil used was replaced with: microbial oil extracted from microbial fermentation broth A, B, D by an acid heating method according to a volume ratio of 1:1:1.
Comparative example 6
A microbial oil liquid lubricant for drilling fluids was prepared as in example 4, except that the microbial oil used was replaced with: microbial oil extracted from microbial fermentation broth B, C, D by an acid heating method according to a volume ratio of 1:1:1.
Evaluation of Performance
(1) Lubricating property
400mL of prepared fresh water-based slurry is respectively added into a No. 0 high-stirring cup and a No. 1 high-stirring cup, 4g of the microbial oil liquid lubricant for drilling fluid provided by examples 2-10 and comparative examples 1-6 is respectively added into the No. 1-15 high-stirring cup-based slurry, and the mixture is stirred for 5min and poured into a No. 0 aging tank and a No. 1 aging tank; and then the aging tank is thermally rolled for 16 hours in a roller heating furnace at 120 ℃, taken out and cooled to room temperature, stirred for 5 minutes at high temperature, and the extreme pressure lubrication coefficient of the aging tank is measured (the aging tank is tested by adopting a digital display type extreme pressure lubrication instrument of OFITE provided by the national science and technology Co., ltd.). The measured results show the reduction rate of the lubricating coefficient in the fresh water slurry in Table 2.
400mL of prepared fresh water base slurry is respectively added into a No. 0 high stirring cup and a No. 1 high stirring cup to 15 high stirring cups, 16g of NaCl is added into the No. 0 high stirring cup and the No. 1 high stirring cup, 4g of the microbial oil liquid lubricant for drilling fluid provided by examples 2 to 10 and comparative examples 1 to 6 is respectively added into the No. 1 high stirring cup base slurry to 15 high stirring cup, the mixture is stirred for 5min, and the mixture is poured into a No. 0 aging tank and a No. 1 aging tank to 15 aging tank; and then the aging tank is thermally rolled for 16 hours in a roller heating furnace at 120 ℃, taken out and cooled to room temperature, stirred for 5 minutes, and the extreme pressure lubrication coefficient (the detection instrument is the same as above) is measured. The measured results show the reduction rate of the lubricating coefficient in 4% brine slurry in Table 2.
400mL of prepared fresh water base slurry is respectively added into a No. 0 high stirring cup and a No. 1 high stirring cup to 15 high stirring cups, 144g of NaCl is added into the No. 0 high stirring cup and the No. 1 high stirring cup, 4g of the microbial oil liquid lubricant for drilling fluid provided by examples 2 to 10 and comparative examples 1 to 6 is respectively added into the No. 1 high stirring cup base slurry to 15 high stirring cup, the mixture is stirred for 5min, and the mixture is poured into a No. 0 aging tank and a No. 1 aging tank to 15 aging tank; and then the aging tank is thermally rolled for 16 hours in a roller heating furnace at 120 ℃, taken out and cooled to room temperature, stirred for 5 minutes, and the extreme pressure lubrication coefficient (the detection instrument is the same as above) is measured. The results of the measurement are shown in Table 2 as the reduction rate of the lubricating coefficient in the saturated brine slurry.
In the detection, the preparation method of the dilute aqueous slurry specifically comprises the following steps: 400mL of distilled water is added into a high stirring cup, 0.8g of anhydrous sodium carbonate and 20g of sodium bentonite are added under stirring, the high stirring is carried out for 20min, the stirring is interrupted at least twice to scrape off the adhesion substance adhered on the wall of the cup, the sealing maintenance is carried out for 24h at 25+/-1 ℃ to ensure that the apparent viscosity reaches 13-15 mpa.s, and if the addition amount of bentonite cannot be properly regulated.
The extreme pressure lubrication coefficient reduction rate calculation formula is:
wherein:
k: extreme pressure lubrication coefficient reduction rate;
K base group : extreme pressure lubrication coefficient of drilling fluid without lubricant;
K moistening device : extreme pressure lubrication coefficient of drilling fluid to which lubricant has been added.
TABLE 2 evaluation results of lubricating Properties of microbial lipid liquid Lubricants prepared by the present invention
In the results of table 2, the reduction rate of the fresh water slurry lubrication coefficient of the microbial oil liquid lubricant provided by the preferred embodiment of the invention under the high temperature condition of 120 ℃ is more than 78%, the reduction rate of the 4% saline slurry lubrication coefficient is more than 75%, and the reduction rate of the saturated saline slurry lubrication coefficient is more than 60%, which indicates that the microbial oil liquid lubricant provided by the preferred embodiment of the invention has better lubrication performance.
(2) Fluorescence level
The microbial oil liquid lubricants provided in examples 2 to 10 and comparative examples 1 to 6 of the present invention were subjected to fluorescence level detection by the following method:
(1) taking a clean test tube 1# to 5# for standby;
(2) using an analytical balance to take 0.010g of the lubricant samples provided in examples 2-10 and comparative examples 1-6 respectively, putting the lubricant samples into a No. 1 test tube, adding isopropanol to 10ml, soaking for about 15 minutes to completely dissolve the lubricant samples, wherein the concentration of the solution in the No. 1 test tube is 1000mg/L;
(3) taking 50ul of solution from a 1# test tube by using a micro liquid taking device, adding the solution into a 2# test tube, adding isopropanol to 10ml, and shaking uniformly, wherein the concentration of the solution in the 2# test tube is 5mg/L;
(4) taking 100ul of solution from a 1# test tube by using a micro liquid taking device, adding the solution into a 3# test tube, adding isopropanol to 10ml, and shaking uniformly, wherein the concentration of the solution in the 3# test tube is 10mg/L;
(5) taking 150ul of solution from the No. 1 test tube by using a micro liquid taking device, adding the solution into the No. 4 test tube, adding isopropanol to 10ml of the solution, and shaking the solution uniformly, wherein the concentration of the solution in the No. 4 test tube is 15mg/L;
(6) 200ul of solution is taken from a No. 1 test tube by a micro liquid taking device and added into a No. 5 test tube, isopropanol is added until 10ml of the solution is uniformly shaken, and the concentration of the solution in the No. 5 test tube is 20mg/L;
(7) and (3) respectively pouring the samples with different concentrations prepared in the steps (3) to (6) into a cuvette, setting three-dimensional scanning parameters for scanning, storing map data (the measuring instrument is a QFA-3DII three-dimensional quantitative fluorescence analyzer provided by Beijing Yongsheng general science and technology development Co., ltd.), and calculating the fluorescence level. The results of the fluorescence level measurement are shown in Table 3.
TABLE 3 evaluation results of fluorescence grades of microbial lipid liquid lubricants prepared by the present invention
The results in Table 3 show that the microbial oil liquid lubricants provided in the preferred embodiments 2-8 of the present invention have a fluorescence level of < 3, and have no effect on analysis and evaluation of formation data.
(3) Apparent viscosity
The apparent viscosity of the microorganism grease liquid lubricants provided in examples 2 to 10 and comparative examples 1 to 6 of the present invention was measured after the fresh water base slurry was added, and the measurement steps were as follows:
400mL of fresh water slurry is measured in a No. 1-4 high stirring cup, 4g of the microbial oil liquid lubricant provided in examples 2-10 and comparative examples 1-6 are added in the No. 1 high stirring cup, the No. 2 high stirring cup, the No. 3 high stirring cup and the No. 4 high stirring cup respectively, the high stirring is carried out for 5min, 600 read values (the measuring instrument is a ZNN-D6B type electric six-speed viscometer provided by Qingdades cis electronic mechanical Co., ltd.) of the No. 1 slurry are measured, and the apparent viscosity value is calculated, and the apparent viscosity difference value of the No. 2 slurry, the No. 3 slurry, the No. 4 slurry and the No. 1 slurry is the apparent viscosity rise value. The preparation method of the fresh water-based slurry is the same as that of the fresh water-based slurry when the lubrication coefficient is measured.
The measurement results are shown in Table 4.
TABLE 4 apparent viscosity evaluation results of the microbial lipid liquid lubricants prepared by the present invention
The results in table 4 show that the apparent viscosity of the microbial oil liquid lubricant provided by the examples of the present invention is slightly reduced after the microbial oil liquid lubricant is added into the fresh water base slurry, so that the microbial oil liquid lubricant provided by the examples of the present invention has no obvious influence on the rheological property of drilling fluid.
(4) Medium pressure fluid loss
The liquid lubricants of the microbial oils and fats provided in examples 2 to 10 and comparative examples 1 to 6 of the present invention were added to fresh water base slurry, and then the filtration loss under pressure was measured as follows:
400mL of fresh water slurry is measured in a No. 1-4 high stirring cup, 4g of microbial oil liquid lubricant provided in examples 2-10 and comparative examples 1-6 are added in the No. 1-4 high stirring cup, the high stirring is carried out for 5min, the medium pressure filtration loss of the No. 1-4 slurry is measured (the measuring instrument is SD-4 type multi-medium pressure filtration loss instrument provided by Qingdao cis electronic mechanical Co., ltd.), and the medium pressure filtration loss difference value of the No. 2, no. 3, no. 4 slurry and the No. 1 slurry is the filtration loss increase value. The preparation method of the fresh water-based slurry is the same as that of the fresh water-based slurry when the lubrication coefficient is measured.
The measurement results are shown in Table 5.
TABLE 5 evaluation results of medium pressure fluid loss of microbial lipid liquid lubricants prepared by the present invention
In the results of table 5, the microbial oil liquid lubricant provided in the preferred embodiment of the present invention has a small change in fluid loss after being added to the fresh water-based slurry, indicating that the microbial oil liquid lubricant provided in the preferred embodiment of the present invention is advantageous for stabilizing the well wall.
(5) Foaming ratio
The foaming rate of the microbial oil liquid lubricants provided in examples 2 to 10 and comparative examples 1 to 6 of the present invention was measured as follows:
400mL of cured fresh water slurry is respectively taken in a No. 1-3 high stirring cup, 4g of the microbial oil liquid lubricant provided in examples 2-10 and comparative examples 1-6 are respectively added, high stirring is carried out for 5min, timing is started after stirring is stopped, the slurry is poured into a 1000mL measuring cylinder within 20s, the volume is read out within 30s, and the foaming rate is calculated. The preparation method of the fresh water-based slurry is the same as that of the fresh water-based slurry when the lubrication coefficient is measured.
The measurement results are shown in Table 6.
TABLE 6 evaluation results of foaming Rate of microbial lipid liquid lubricants prepared by the present invention
In the results of table 6, after the microbial oil liquid lubricant provided in the examples of the present invention is added to the fresh water base slurry, the foaming rate is less than 4.0%, which indicates that the microbial oil liquid lubricant provided in the examples of the present invention has good compatibility with water-based drilling fluid and does not substantially foam.
(6) Biotoxicity of
The microbial oil liquid lubricants provided in examples 2 to 10 and comparative examples 1 to 6 of the present invention were subjected to biotoxicity detection by a luminescent bacterial method, and the detection method is as follows:
firstly, preparing a microbial oil liquid lubricant into 200mL of 50000mg/L solution by using 3% sodium chloride solution, stirring for 30min at 12000rpm to enable a sample to be fully dissolved, preparing 10mL of each of 0, 10000mg/L, 25000mg/L, 50000mg/L, 100000mg/L and 200000mg/L to be tested microbial oil liquid lubricant sample solutions by using the solution, and standing for 60min; then, 10mg of luminescent bacteria T3 powder is sequentially added into the diluted solution, fully and uniformly vibrated, and the relative luminescence value EC after the luminescent bacteria and the sample are contacted for 15min is respectively measured by using 3% sodium chloride solution as a contrast 50 (15 min) was experimentally determined. The measurement results are shown in Table 7.
TABLE 7 evaluation results of biotoxicity of microbial lipid liquid lubricants prepared by the present invention
| Biotoxicity EC 50 ，mg/L | |
| Example 2 | 115900 |
| Example 3 | 128000 |
| Example 4 | 139000 |
| Example 5 | 113500 |
| Example 6 | 114400 |
| Example 7 | 137700 |
| Example 8 | 126600 |
| Example 9 | 97000 |
| Example 10 | 108000 |
| Comparative example 1 | 88300 |
| Comparative example 2 | 92000 |
| Comparative example 3 | 93200 |
| Comparative example 4 | 94600 |
| Comparative example 5 | 96700 |
| Comparative example 6 | 98200 |
Table 7 results of the invention in the preferred embodiment of the microbial oil liquid lubricant samples EC 50 The value is more than 110000mg/L (which is far more than 30000mg/L of nontoxic index specified by national environmental protection), which indicates that the microbial oil liquid lubricant provided by the embodiment of the invention is green and nontoxic.
The experimental detection proves that the microbial oil liquid lubricant for the drilling fluid provided by the embodiment of the invention has good stability in water-based drilling fluid, can obviously reduce the extreme pressure lubrication coefficient, the lubrication coefficient reduction rate in fresh water slurry is more than or equal to 78%, the lubrication coefficient reduction rate in 4% brine slurry is more than or equal to 75%, and the lubrication coefficient reduction rate in saturated brine slurry is more than or equal to 60%; when the addition amount of the base slurry is 1%, the apparent viscosity change is small, the viscosity is not increased, the foaming rate is less than 4%, the compatibility in the water-based drilling fluid is good, the rheological property of the drilling fluid is not obviously affected, and the base slurry plays a vital role in reducing the underground complex conditions such as stuck drilling and the like and ensuring safe, high-quality and quick drilling; no toxicity or low toxicity, low fluorescence, the fluorescence level is less than 3, no corrosion to metal, no pollution to environment, biodegradability and no influence on analysis and evaluation of stratum data; the filtration loss is basically unchanged, which is beneficial to stabilizing the well wall; biotoxicity EC 50 The value is more than 110000mg/L, and the environmental protection performance is good.
From the above examples, the present invention preferably provides a microbial oil liquid lubricant for drilling fluid, which comprises the following materials in parts by weight: 70-90 parts of microbial oil; 1-3 parts of antiwear agent; 3-7 parts of stabilizer; 3-5 parts of emulsifying agent; the preparation method of the microbial oil comprises the following steps: culturing 4 different oleaginous strains to obtain microbial fermentation liquid, mixing the 4 microbial fermentation liquid according to a volume ratio to obtain microbial fermentation mixed liquid, centrifugally separating the microbial fermentation mixed liquid to obtain microbial thalli, and finally obtaining microbial grease by an acid heating method. The microbial oil liquid lubricant for the drilling fluid provided by the invention has the advantages of good lubricating effect, no fluorescence, no toxicity or low toxicity, environmental protection, easiness in biodegradation and good compatibility with a drilling fluid system, and is suitable for vertical wells, inclined wells, horizontal wells and the like.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
While the invention has been described with respect to the preferred embodiments, it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention and are intended to be within the scope of the present invention.
Claims (7)
1. The microbial oil liquid lubricant for the drilling fluid is characterized by comprising the following raw materials of microbial oil, a stabilizer, an emulsifier and an antiwear agent; the stabilizer is selected from stearate, the microbial oil is prepared by respectively culturing 4 oleaginous strains, and the 4 oleaginous strains are respectively oleaginous yeast of St. Cover, candida rugosa, rhodotorula glutinis and ascomycete oleaginous yeast;
the microbial oil is prepared according to the following steps:
culturing the 4 oleaginous strains respectively to obtain 4 corresponding microbial fermentation broths respectively; mixing the 4 microbial fermentation liquids according to the volume ratio of 1:1:1:1 to obtain a microbial fermentation mixed liquid, and centrifugally separating the microbial fermentation mixed liquid to obtain microbial thalli; finally, extracting the microbial thalli by an acid heating method to obtain microbial grease; the temperature for respectively culturing the 4 oleaginous strains is 24-30 ℃, and the culture time is more than 24 hours.
2. The microbial oil liquid lubricant for drilling fluids according to claim 1, wherein the stabilizer is one or more of calcium stearate, zinc stearate, sodium stearate, magnesium stearate and barium stearate.
3. The microbial oil liquid lubricant for drilling fluid according to claim 1, wherein the emulsifier is one or more of sodium dodecylbenzene sulfonate, fatty acid methyl ester sulfonate, succinic acid ester sulfonate, span 80 and tween 85.
4. The microbial oil liquid lubricant for drilling fluids according to claim 3, wherein the antiwear agent is one or more of vulcanized lard, vulcanized cottonseed oil, vulcanized fatty acid ester and triethanolamine borate.
5. The microbial oil liquid lubricant for drilling fluid according to claim 4, which is composed of the following raw materials in parts by weight:
70-90 parts of microbial oil; 1-3 parts of antiwear agent; 3-7 parts of stabilizer; 3-5 parts of emulsifying agent.
6. A method for producing the microbial oil liquid lubricant for drilling fluids according to any one of claims 1 to 5, comprising: mixing the microbial oil and other raw materials according to the weight ratio for more than 5 hours at the temperature of 50-80 ℃ and the stirring speed of 300-600 rpm to obtain the microbial oil liquid lubricant for drilling fluid.
7. Use of the microbial oil liquid lubricant for drilling fluids according to any one of claims 1 to 5 in the preparation of drilling fluids.
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