CN115245939A

CN115245939A - pcr reagent preparing rack

Info

Publication number: CN115245939A
Application number: CN202210000711.3A
Authority: CN
Inventors: 曹思; 张丽; 杨晓彤; 陈大志
Original assignee: Heilongjiang Noah Ark Gene Technology Co ltd
Current assignee: Heilongjiang Noah Ark Gene Technology Co ltd
Priority date: 2022-01-04
Filing date: 2022-01-04
Publication date: 2022-10-28

Abstract

The invention provides a pcr reagent preparation frame, and relates to the field of biological and medical experiments. This frame is prepared to pcr reagent, including the chassis, the equal fixedly connected with flank board in chassis up end both sides, two close to the front end department between the flank board and all rotate and be connected with the connection pivot, it is connected with the jacking cylinder to rotate on the chassis, chassis rear end face fixedly connected with electricity jar backup pad, fixed regulation has the configuration box on the connection pivot outer wall, the fixed inside groove of having seted up of terminal surface before the configuration box, the fixed display screen that is provided with of terminal surface before the inside groove, the terminal surface is fixed waterproof board that is provided with before the display screen, the fixed a plurality of configuration test tubes that are provided with of configuration box internal fixation, the fixed cover of configuration test tube outer wall is equipped with the heat preservation. The invention provides a pcr reagent preparation frame which has an autonomous cleaning function, does not need manual operation, is simple and trouble-saving, and can display prompt messages through a display screen, thereby being convenient for prompting configuration personnel and improving configuration efficiency.

Description

pcr reagent preparing rack

Technical Field

The invention relates to the field of biological and medical experiments, in particular to a pcr reagent preparation rack.

Background

PCR (polymerase chain reaction) has the characteristics of high sensitivity and strong specificity, has become a key technology for molecular biology research as a mature technology, and is widely applied to various experiments of bioscience and medicine. The basic principle is that based on the DNA semi-retention mechanism in vivo cell division, double-stranded DNA is made to unwind into single strand by making the temperature of an in vitro synthesis system, and then the single-stranded DNA is renatured with artificially designed primer, and the DNA polymerase is replicated into double-stranded DNA along a single-stranded template according to the position of the primer. The whole PCR process consists of three steps of high temperature denaturation, low temperature renaturation and certain temperature extension.

In a conventional PCR experiment, reagents such as DNA samples and primers are usually placed in a common 384-well or 96-well plastic substrate according to requirements, and a transparent plastic film is attached to the surface of the substrate to enclose the solution system. After the reciprocating temperature of the PCR experiment platform changes, the analysis is carried out by a common light source or laser penetrating through a plastic film. In a PCR experiment, the temperature of a DNA sample is generally required to change along with the temperature of PCR, and the higher the temperature change sensitivity from high temperature to low temperature or from low temperature to high temperature in the PCR reaction, the better. Therefore, a suitable PCR kit is urgently needed.

The prior patent (application number: 201620676882.8) discloses a kit suitable for PCR experiments, which comprises a box body, an EP pipe frame, a reagent-containing EP pipe, a sterile enzyme-free water tank and a reserved reagent-containing EP pipe, wherein a box cover is arranged above the box body, the inner wall of the whole body of the box body is provided with a heat insulation layer, the box body is internally divided into three parts by partition plates, the EP pipe frame, the reserved pipe frame and the sterile enzyme-free water tank are respectively placed in the box body, the EP pipe frame is in a plate shape similar to a 96-hole plate shape, the reagent-containing EP pipe is placed on the EP pipe frame, the reserved reagent-containing EP pipe is placed on the reserved pipe frame, a pipe cover is arranged above the reagent-containing EP pipe, the pipe cover and the pipe body are respectively provided with digital labels, and the reagent-containing EP pipe is internally provided with PCR reaction system premixed reagents of 20uL, 35uL, 40uL and 50uL standards; the kit is provided.

Although simple structure, access convenience make reagent prepare process and application of sample process obtain simplifying, have good heat preservation function, prepare the frame with current reagent on the market similarly, have the trouble problem of clearance, and need record corresponding data in order to prevent making mistakes during the configuration, present device can only carry out the record through handwriting basically, lacks corresponding prompt facility, and based on this, we propose pcr reagent and prepare the frame.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a pcr reagent preparation frame, which solves the problems that the prior device is troublesome to clean after being used and lacks a prompt function.

(II) technical scheme

In order to achieve the purpose, the invention is realized by the following technical scheme: frame is prepared to pcr reagent, including the chassis, the equal fixedly connected with side wing board in chassis up end both sides, two near the front end department between the side wing board all rotates and is connected with the connection pivot, it is connected with the jacking cylinder to rotate on the chassis, chassis rear end face fixedly connected with electricity jar backup pad, fixed the adjusting has the configuration box on the connection pivot outer wall, the fixed inside groove of having seted up of terminal surface before the configuration box, the fixed display screen that is provided with of terminal surface before the inside groove, the fixed waterproof board that is provided with of terminal surface before the display screen, the fixed a plurality of configuration test tubes that are provided with of configuration box interior, the fixed cover that is equipped with of configuration test tube outer wall, the configuration box is close to the fixed cleaning layer that is provided with of upper end department, the fixed scavenge pipe that is provided with in the cleaning layer, cleaning layer one side fixedly connected with inlet tube, inlet tube and scavenge pipe intercommunication set up.

The utility model discloses a cleaning brush, including chassis, electric jar backup pad, horizontal adjusting screw, vertical adjusting screw outer wall, chassis rear end face fixedly connected with electricity jar backup pad, electricity jar backup pad up end difference fixedly connected with telescopic link and electronic jar, the horizontal adjusting plate of telescopic link and electronic jar up end fixedly connected with, the fixed horizontal adjusting spout that has seted up of terminal surface before the horizontal adjusting plate, it is connected with horizontal adjusting screw to rotate between the horizontal adjusting screw both sides inner wall, horizontal adjusting screw outer wall upper thread has cup jointed horizontal adjusting block, the vertical regulating plate of terminal surface fixedly connected with before the horizontal adjusting block, vertical adjusting plate one side is fixed has seted up vertical adjusting spout, it is connected with vertical adjusting screw to rotate between the inner wall around the vertical adjusting spout, vertical adjusting screw has cup jointed vertical adjusting block in the screw on the outer wall, vertical adjusting block one side fixedly connected with mounting panel, fixedly connected with adjust the cylinder on the mounting panel, adjust the cylinder output runs through mounting panel and fixedly connected with driving motor, driving motor lower extreme screw has cup jointed cleaning brush.

Preferably, be provided with wireless module, control module and display module on the device respectively, display screen and display module electric connection, wireless module, display module, jacking cylinder, electronic jar, vertical motor, adjust the cylinder, driving motor and horizontal motor all with control module electric connection.

Preferably, the transverse adjusting slide block is slidably connected to the inner end of the transverse adjusting chute, and the longitudinal adjusting slide block is slidably connected to the inner end of the longitudinal adjusting chute.

Preferably, a recording plate is fixedly connected to the rear end face of the configuration box near the upper part.

Preferably, the heat-insulating layer is made of transparent heat-insulating materials.

Preferably, the waterproof plate is fixedly arranged at the inner end of the inner groove.

Preferably, the configuration box and the waterproof plate are both made of transparent glass materials.

Preferably, the fixed horizontal motor that is provided with in horizontal regulating plate one side, horizontal motor output and horizontal accommodate the lead screw fixed connection, vertical regulating plate is close to the inside fixed vertical motor that is provided with of front end, vertical motor output and vertical accommodate the lead screw fixed connection.

Preferably, the bottom of the configuration box is hinged with the output end of the jacking cylinder.

The working principle is as follows: when the pcr reagent preparation rack is used, terminal information is received through the wireless module, the terminal information is coded through the control module, and then the terminal information is displayed on a display screen through the display module for reminding; when later stage is clean, it rotates to drive horizontal accommodate the lead screw through horizontal motor, realize driving the motion of horizontal adjusting block, and then drive the motion of longitudinal adjustment board, the rethread longitudinal adjustment motor drives the motion of longitudinal adjustment lead screw, and then drive the motion of longitudinal adjustment slider, and then drive the actuating cylinder motion through mounting panel fixed mounting, finally realize driving the clean brush under it and carry out the kinematic positioning, then rotate through the clean brush of driving motor drive, the cooperation actuating cylinder carries out lift adjustment, the realization is cleaned the configuration test tube inner wall of difference, clean the back that finishes, clean brush moves to configuration box one side, will dispose the box through the jacking cylinder, the jack-up is turned over to the box body slant downwards, the evacuation washs the waste liquid can.

(III) advantageous effects

The invention provides a pcr reagent preparation frame. The method has the following beneficial effects:

1. the invention provides a pcr reagent preparation frame, which is characterized in that the autonomous movement of a cleaning brush is controlled by arranging a transverse adjusting mechanism and a longitudinal adjusting mechanism, so that the inner wall of a preparation test tube is conveniently cleaned, and the cleaning effect is better and more complete by matching with an adjusting cylinder and performing up-down lifting adjustment during cleaning.

2. The invention provides a pcr reagent preparation frame, which is connected with external terminal equipment through a wireless module, can realize remote data transmission, further conveniently sends prompting and recording information to a device through the terminal equipment, and reminds configuration personnel through display of a display screen.

3. The invention provides a pcr reagent preparation frame, which is characterized in that a jacking cylinder is arranged, and a water inlet pipe is matched to be connected into external water supply equipment, so that after the inner wall of a test tube is cleaned and prepared, cleaning liquid in the test tube can be jacked and discharged conveniently. Manual control is not needed, and the labor is saved.

4. The invention provides a pcr reagent preparation frame, and in conclusion, the device has an autonomous cleaning function, does not need manual operation, is simple and trouble-saving, displays prompt messages through a display screen, is convenient for prompting configuration personnel, and improves configuration efficiency.

Drawings

FIG. 1 is a schematic perspective view of the present invention;

FIG. 2 is a side sectional view of a portion of the present invention;

FIG. 3 is a schematic top view of the bottom frame of the present invention;

FIG. 4 is a schematic view of the internal structure of a cleaning layer according to the present invention;

FIG. 5 is a schematic view of a module connection structure according to the present invention;

fig. 6 is a schematic view of the working structure of the jacking cylinder of the present invention.

Wherein, 1, a chassis; 2. a side wing panel; 3. connecting the rotating shaft; 4. jacking a cylinder; 5. an electric cylinder support plate; 6. a configuration box; 7. a telescopic rod; 8. an electric cylinder; 9. a transverse adjusting plate; 10. a transverse adjusting chute; 11. a transverse adjusting screw rod; 12. a transverse adjusting slide block; 13. a longitudinal adjusting plate; 14. a longitudinal adjusting chute; 15. a longitudinal adjusting screw rod; 16. a longitudinal motor; 17. a longitudinal adjusting slide block; 18. mounting a plate; 19. an adjusting cylinder; 20. a drive motor; 21. cleaning the brush; 22. an inner tank; 23. a display screen; 24. a waterproof sheet; 25. preparing a test tube; 26. a heat-insulating layer; 27. a recording plate; 28. cleaning the layer; 29. cleaning the pipe; 30. a water inlet pipe; 31. a transverse motor; 32. a wireless module; 33. a control module; 34. and a display module.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example (b):

as shown in fig. 1 to 6, an embodiment of the invention provides a pcr reagent preparation frame, which includes a chassis 1, two sides of an upper end surface of the chassis 1 are fixedly connected with side wing plates 2, a connecting rotating shaft 3 is rotatably connected between the two side wing plates 2 near a front end, a jacking cylinder 4 is rotatably connected to the chassis 1, a rear end surface of the chassis 1 is fixedly connected with an electric cylinder support plate 5, a configuration box 6 is fixedly adjusted on an outer wall of the connecting rotating shaft 3, an inner groove 22 is fixedly formed on a front end surface of the configuration box 6, a display screen 23 is fixedly arranged on a front end surface of the inner groove 22, a waterproof plate 24 is fixedly arranged on a front end surface of the display screen 23, a plurality of configuration test tubes 25 are fixedly arranged in the configuration box 6, an insulating layer 26 is fixedly sleeved on an outer wall of the configuration test tube 25, a cleaning layer 28 is fixedly arranged at a position near an upper end of the configuration box 6, a cleaning tube 29 is fixedly arranged in the cleaning layer 28, a water inlet tube 30 is fixedly connected to one side of the cleaning layer 28, and the water inlet tube 30 is communicated with the cleaning tube 29.

1 rear end face fixedly connected with electric jar backup pad 5 of chassis, electric jar backup pad 5 up end both sides are fixedly connected with telescopic link 7 and electronic jar 8 respectively, telescopic link 7 and the horizontal regulating plate 9 of electronic jar 8 up end fixedly connected with, horizontal regulation spout 10 has been seted up to the fixed terminal surface before the horizontal regulating plate 9, it is connected with horizontal accommodate the lead screw 11 to rotate between the both sides inner wall of horizontal regulation spout 10, horizontal accommodate the lead screw 11 outer wall on the screw thread cup joints horizontal regulating block 12, terminal surface fixedly connected with vertical regulating plate 13 before horizontal regulating block 12, vertical regulating plate 13 one side is fixed has seted up vertical regulation spout 14, it is connected with vertical regulating screw 15 to rotate between the inner wall around vertical regulating spout 14, vertical regulating screw 15 outer wall on the screw thread cup joint vertical regulating block 17, vertical regulating block 17 one side fixedly connected with mounting panel 18, fixedly connected with adjust cylinder 19 on mounting panel 18, adjust cylinder 19 output runs through mounting panel 18 and fixedly connected with driving motor 20, terminal screw thread has cup jointed clean brush 21 under driving motor 20.

Be provided with wireless module 32, control module 33 and display module 34 on the device respectively, display screen 23 and display module 34 electric connection, wireless module 32, display module 34, jacking cylinder 4, electronic jar 8, vertical motor 16, adjust the cylinder 19, driving motor 20 and horizontal motor 31 all with control module 33 electric connection, the device passes through wireless module 32 and realizes data reception.

The transverse adjusting slide block 12 is connected to the inner end of the transverse adjusting slide groove 10 in a sliding mode, the transverse adjusting slide block 12 is prevented from rotating relative to the transverse adjusting slide groove 10 when moving, the longitudinal adjusting slide block 17 is connected to the inner end of the longitudinal adjusting slide groove 14 in a sliding mode, and the longitudinal adjusting slide block 17 is prevented from rotating relative to the longitudinal adjusting slide groove 14 when moving.

The rear end face of the configuration box 6 is fixedly connected with a recording plate 27 close to the upper part for assisting in recording information and facilitating memory.

The heat-insulating layer 26 is made of transparent heat-insulating material.

The waterproof board 24 is fixedly arranged at the inner end of the inner groove 22, and the display screen 23 and the internal electric appliance module are protected by the waterproof board 24.

The configuration box 6 and the waterproof plate 24 are both made of transparent glass materials, so that a configurator can conveniently observe the configuration box.

A transverse motor 31 is fixedly arranged on one side of the transverse adjusting plate 9, the output end of the transverse motor 31 is fixedly connected with a transverse adjusting screw rod 11, the transverse motor 31 drives the transverse adjusting screw rod 11 to rotate, the transverse adjusting slider 12 is driven to move, a longitudinal motor 16 is fixedly arranged in the longitudinal adjusting plate 13 close to the front end, the output end of the longitudinal motor 16 is fixedly connected with a longitudinal adjusting screw rod 15, and the longitudinal adjusting screw rod 15 is driven to rotate through the longitudinal motor 16, so that the longitudinal adjusting slider 17 is driven to move.

The bottom of the configuration box 6 is hinged with the output end of the jacking cylinder 4, and the configuration box 6 is driven to rotate and turn over by the action of the jacking cylinder 4.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

The pcr reagent preparation frame comprises a bottom frame (1), and is characterized in that: both sides of the upper end face of the bottom frame (1) are fixedly connected with side wing plates (2), a connecting rotating shaft (3) is rotatably connected between the two side wing plates (2) close to the front end, a jacking cylinder (4) is rotatably connected to the bottom frame (1), an electric cylinder supporting plate (5) is fixedly connected to the rear end face of the bottom frame (1), a configuration box (6) is fixedly adjusted on the outer wall of the connecting rotating shaft (3), an inner groove (22) is fixedly formed in the front end face of the configuration box (6), a display screen (23) is fixedly arranged on the front end face of the inner groove (22), a waterproof plate (24) is fixedly arranged on the front end face of the display screen (23), a plurality of configuration test tubes (25) are fixedly arranged in the configuration box (6), a heat preservation layer (26) is fixedly arranged on the outer wall of the configuration test tubes (25), a cleaning layer (28) is fixedly arranged on the position, close to the upper end, a cleaning pipe (29) is fixedly arranged in the cleaning layer (28), one side of the cleaning layer (28) is fixedly connected with a water inlet pipe (30) communicated with the cleaning pipe (29);

the rear end face of the underframe (1) is fixedly connected with an electric cylinder supporting plate (5), both sides of the upper end surface of the electric cylinder supporting plate (5) are respectively and fixedly connected with a telescopic rod (7) and an electric cylinder (8), the upper end surfaces of the telescopic rod (7) and the electric cylinder (8) are fixedly connected with a transverse adjusting plate (9), a transverse adjusting sliding groove (10) is fixedly arranged on the front end surface of the transverse adjusting plate (9), a transverse adjusting screw rod (11) is rotatably connected between the inner walls at the two sides of the transverse adjusting chute (10), a transverse adjusting sliding block (12) is sleeved on the outer wall of the transverse adjusting screw rod (11) in a threaded manner, the front end surface of the transverse adjusting slide block (12) is fixedly connected with a longitudinal adjusting plate (13), one side of the longitudinal adjusting plate (13) is fixedly provided with a longitudinal adjusting sliding groove (14), a longitudinal adjusting screw rod (15) is rotationally connected between the front inner wall and the rear inner wall of the longitudinal adjusting chute (14), a longitudinal adjusting sliding block (17) is sleeved on the outer wall of the longitudinal adjusting screw rod (15) in a threaded manner, one side of the longitudinal adjusting slide block (17) is fixedly connected with a mounting plate (18), an adjusting cylinder (19) is fixedly connected on the mounting plate (18), the output end of the adjusting cylinder (19) penetrates through the mounting plate (18) and is fixedly connected with a driving motor (20), the lower end of the driving motor (20) is sleeved with a cleaning brush (21) in a threaded manner.
2. The pcr reagent dispensing rack of claim 1, wherein: be provided with wireless module (32), control module (33) and display module (34) on the device respectively, display screen (23) and display module (34) electric connection, wireless module (32), display module (34), jacking cylinder (4), electronic jar (8), vertical motor (16), adjust cylinder (19), driving motor (20) and horizontal motor (31) all with control module (33) electric connection.
3. The pcr reagent formulation rack of claim 1, wherein: the transverse adjusting slide block (12) is connected to the inner end of the transverse adjusting slide groove (10) in a sliding mode, and the longitudinal adjusting slide block (17) is connected to the inner end of the longitudinal adjusting slide groove (14) in a sliding mode.
4. The pcr reagent dispensing rack of claim 1, wherein: a recording plate (27) is fixedly connected to the rear end face of the configuration box (6) close to the upper part.
5. The pcr reagent dispensing rack of claim 1, wherein: the heat-insulating layer (26) is made of transparent heat-insulating materials.
6. The pcr reagent dispensing rack of claim 1, wherein: the waterproof plate (24) is fixedly arranged at the inner end of the inner groove (22).
7. The pcr reagent dispensing rack of claim 1, wherein: the configuration box (6) and the waterproof plate (24) are both made of transparent glass materials.
8. The pcr reagent formulation rack of claim 1, wherein: horizontal regulating plate (9) one side is fixed and is provided with horizontal motor (31), horizontal motor (31) output and horizontal accommodate the lead screw (11) fixed connection, vertical regulating plate (13) are close to the inside fixed vertical motor (16) that is provided with of front end, vertical motor (16) output and vertical accommodate the lead screw (15) fixed connection.
9. The pcr reagent dispensing rack of claim 1, wherein: the bottom of the configuration box (6) is hinged with the output end of the jacking cylinder (4).