CN115232747B - Full-automatic stem cell culture and expansion device based on microflow control - Google Patents
Full-automatic stem cell culture and expansion device based on microflow control Download PDFInfo
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- CN115232747B CN115232747B CN202211072109.7A CN202211072109A CN115232747B CN 115232747 B CN115232747 B CN 115232747B CN 202211072109 A CN202211072109 A CN 202211072109A CN 115232747 B CN115232747 B CN 115232747B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/02—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by impregnation, e.g. using swabs or loops
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
Abstract
The invention discloses a full-automatic stem cell culture and expansion device based on micro-flow control, and relates to the technical field of cell culture; in order to solve the problem of oxygen supply reliability; specifically including the base and be used for cultivateing the incubator of cell, the bottom of incubator is connected with the vibrations seat through the linkage oxygen supply spare, and the one end rotation of vibrations seat is connected in the base, and the other end of vibrations seat is connected in the base through the vibrations spare, the bottom of incubator is provided with the linkage oxygen supply spare, the vibrations seat is connected in the incubator through quick connector. According to the invention, the linkage oxygen supply part is arranged, so that oxygen can be supplied into the incubator, and the oxygen supply power is linked with vibration through the piston and the piston rod, so that the oxygen supply rate is positively correlated with vibration frequency, and the single oxygen supply quantity is positively correlated with the amplitude, thereby realizing the linkage of micro-flow control and oxygen supply in a vibration mode, and ensuring the reliability of oxygen supply.
Description
Technical Field
The invention relates to the technical field of cell culture, in particular to a fully-automatic stem cell culture expansion device based on micro-flow control.
Background
The stem cell culture is in-vitro expansion culture of stem cells by using cell culture equipment, and the stem cells are subjected to expansion culture by using a culture medium and a nutrient solution.
Through retrieving, chinese patent publication No. CN113481098A discloses a full-automatic stem cell culture expansion device based on micro-fluidic, including micro-fluidic regulation and control mechanism, micro-fluidic regulation and control mechanism's inside is provided with the transmission shaft, micro-fluidic regulation and control mechanism's inside is provided with the rocking disc, the lower surface of rocking disc is provided with the fulcrum, the upper surface sliding connection of rocking disc has the connecting piece, the upper surface rotation of rocking disc is connected with the connecting rod.
The above patent suffers from the following disadvantages: because the greater the vibration energy, the more oxygen loss in the nutrient solution will be accelerated, and only the aeration plate is used for oxygen supply, but the supply speed cannot be mutually linked and matched with the wake flow regulating mechanism (namely, the vibration thereof), so that the internal hypoxia condition may be caused.
Disclosure of Invention
The invention aims to solve the defects in the prior art, and provides a full-automatic stem cell culture and expansion device based on micro-flow control.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
the full-automatic stem cell culture and expansion device based on the micro-flow control comprises a base and an incubator for culturing cells, wherein the bottom of the incubator is connected with a vibration seat through a linkage oxygen supply piece;
the vibration seat is connected to the incubator through a quick connecting piece;
the linkage oxygen supply piece is including being fixed in the inside aeration pipe of incubator and being fixed in the check valve of incubator bottom outer wall, check valve one end is connected with the cylinder body, and the check valve other end passes through the hose connection in the aeration pipe, the outer wall fixed mounting of cylinder body has the check valve two, the inner wall clearance fit of cylinder body has the piston, and the bottom of piston rotates to be connected with the piston rod, and the other end of piston rod rotates to be connected in the top outer wall of base.
Preferably: the aeration pipe comprises a transverse pipe and a plurality of longitudinal pipes communicated with the transverse pipe, wherein uniform aeration holes are formed in the inner wall of one side of the longitudinal pipe, and the aeration holes face to the bottom.
Further: the quick connecting piece includes fixed mounting in the direction slide rail of vibrations seat top outer wall and fixed mounting in incubator bottom outer wall and with direction slide rail sliding fit's direction slider, the inner wall sliding connection of direction slide rail has spacing knot, and spacing knot's bottom outer wall fixed mounting has spring one, and the spacing groove with spacing knot complex is seted up to the inner wall of direction slider.
Based on the scheme: the vibration piece comprises a second spring fixedly installed on the outer wall of one side opposite to the vibration seat and the base, and a cam in contact fit with the outer wall of the bottom of the vibration seat, wherein the cam is rotationally connected to the top of the base through a main shaft, the copper drum support on the outer wall of the top of the base is connected with a motor, and the main shaft is connected with an output shaft of the motor through a coupling.
Among the foregoing, the preferred one is: the cam comprises a wheel body and adjustable protruding rods arranged on two sides of the wheel body, and the two groups of adjustable protruding rods are arranged in a staggered mode.
As a further scheme of the invention: the adjustable convex rod piece comprises a convex rod which is connected with the axial direction of the wheel body in a circular array type in a sliding way, and a ball which is connected with the end part of the convex rod in a rolling way;
the outer wall of the wheel body is fixedly provided with an external thread boss, the outer wall of the external thread boss is movably sleeved with an adjusting disc, the inner wall of the adjusting disc is provided with a chute, and the protruding rod is in limit fit with the inner wall of the chute through a limit rod;
the outer wall of the external thread boss is connected with a pressure plate through threads.
Meanwhile, the nutrient solution concentration control device further comprises a concentration control part, wherein the concentration control part comprises a one-way sealing piece arranged at the bottom of the incubator, a sampling piece for sampling the concentration of the current nutrient solution and a sensing piece for sensing the concentration.
As a preferred embodiment of the present invention: the unidirectional sealing piece comprises a filter plate fixedly arranged on the inner wall of the bottom of the incubator, a conical tube fixedly arranged on the outer wall of the bottom of the incubator and a sealing ball arranged in the conical tube, and a spring III is fixed on the outer wall of one side opposite to the filter plate and the sealing ball;
the sampling piece comprises a lifting rod which is connected to the top of the base in a sliding manner and a cotton head which is fixedly arranged on the top of the lifting rod;
the induction piece comprises a water fading taper pipe fixed on the outer wall of the top of the base and metal spring plate electrodes fixed on two sides of the water fading taper pipe respectively, wherein the two metal spring plate electrodes are connected with the same induction circuit, and the induction circuit comprises a power supply and an electronic ammeter.
Meanwhile, the concentration control part further comprises a high-concentration nutrient solution tank and a power pump, an inlet and an outlet of the power pump are respectively connected with the high-concentration nutrient solution tank and the incubator, and the electronic ammeter is connected with the power pump through an upper computer.
As a more preferable scheme of the invention: the concentration control still includes the linkage piece, the linkage piece includes the lever and sets up in the waist type hole at lever both ends, incubator's bottom outer wall fixed mounting has backup pad two, and the top outer wall fixed mounting of base has backup pad one, backup pad two and lifter all are through spacing post spacing cooperation and waist type hole respectively, the middle part rotation of spacing post is connected in backup pad one.
The beneficial effects of the invention are as follows:
1. according to the invention, the linkage oxygen supply part is arranged, so that oxygen can be supplied into the incubator, and the oxygen supply power is linked with vibration through the piston and the piston rod, so that the oxygen supply rate is positively correlated with vibration frequency, and the single oxygen supply quantity is positively correlated with the amplitude, thereby realizing microflow control and oxygen supply linkage in a vibration mode and ensuring the reliability of oxygen supply.
2. According to the invention, the plurality of vertical pipes are arranged, so that the bottom of the incubator is ensured to be fully paved, the oxygen supply area is uniform, in addition, the aeration holes are arranged towards the bottom, and the nutrient solution sediment at the bottom of the incubator can be floated while the oxygen is supplied, so that the function of preventing sediment is achieved.
3. According to the invention, the incubator can be slid to the top of the vibration seat through the cooperation of the guide sliding block and the guide sliding rail, the incubator is fixed relative to the vibration seat through the mutual clamping of the limit buckle and the limit groove, and after the culture is finished, the incubator can be acted on along the length direction of the guide sliding rail, so that the limit buckle contracts, the quick separation of the incubator and the vibration seat is completed, and the convenience of use is increased.
4. According to the invention, the protruding size of the protruding rod can be changed by rotating the adjusting disc through the adjustable protruding rod piece, so that the change of the amplitude is realized, the two groups of symmetrically arranged adjustable protruding rod pieces can also realize the switching of three modes of high-frequency single-amplitude vibration, low-frequency single-amplitude vibration and high-frequency double-amplitude vibration, and the functionality of the device is improved.
5. According to the invention, the concentration control part is arranged, and the nutrient solution contains a plurality of inorganic salt components, so that the nutrient solution is electrolyte, conductive, the higher the concentration is, the lower the resistivity is, and the concentration is monitored by monitoring the current by utilizing the characteristic, so that the concentration of the nutrient solution is ensured, and the amplification efficiency is improved.
6. According to the invention, the cotton head is arranged for sampling, and the vibration motion of the lever linkage incubator is used for realizing the functions of automatic sampling, automatic closing and automatic sensing, so that the synchronism of the device is improved.
Drawings
FIG. 1 is a schematic diagram of the whole structure of a fully automatic stem cell culture and expansion device based on micro-flow control;
FIG. 2 is a schematic cross-sectional view of a linkage oxygen supply for a fully automatic stem cell culture and expansion device based on micro-flow control according to the present invention;
FIG. 3 is a schematic view of the structure of an aeration tube of a fully automatic stem cell culture expansion device based on microflow control;
FIG. 4 is a schematic cross-sectional view of a quick connector of a fully automatic stem cell culture expansion device based on micro-flow control according to the present invention;
FIG. 5 is a schematic diagram of a vibration member of a fully automatic stem cell culture expansion device based on micro-flow control according to the present invention;
FIG. 6 is a schematic diagram of a cam structure of a fully automatic stem cell culture expansion device based on micro-flow control according to the present invention;
FIG. 7 is a schematic diagram showing the exploded structure of an adjustable male member of a fully automatic stem cell culture expansion device based on micro-flow control according to the present invention;
FIG. 8 is a schematic diagram of a concentration control unit of a fully automatic stem cell culture expansion device based on microflow control according to the present invention;
FIG. 9 is a schematic cross-sectional structure diagram of a concentration control section of a fully automatic stem cell culture expansion device based on microflow control according to the present invention;
FIG. 10 is a schematic diagram of a linkage member of a fully automatic stem cell culture expansion device based on microflow control according to the present invention;
FIG. 11 is a schematic diagram of a sensing circuit of a fully automatic stem cell culture and expansion device based on micro-flow control according to the present invention;
fig. 12 is a schematic diagram of a partial structure of a fully automatic stem cell culture expansion device based on microflow control according to the present invention.
In the figure: 1-incubator, 2-vibration seat, 3-base, 4-quick connector, 5-vibration piece, 6-linkage oxygen supply piece, 7-aeration pipe, 8-check valve one, 9-piston, 10-piston rod, 11-cylinder, 12-check valve two, 13-cross tube, 14-aeration hole, 15-longitudinal tube, 16-guide slide, 17-guide slide rail, 18-limit button, 19-spring one, 20-limit groove, 21-spindle, 22-cam, 23-motor, 24-spring two, 25-wheel body, 26-adjustable cam piece, 27-cam piece, 28-ball, 29-limit rod, 30-external thread boss, 31-adjusting disk, 32-chute, 33-pressure disk, 34-check seal piece, 35-sampling piece, 36-sensing piece, 37-filter plate, 38-spring three, 39-chute, 40-sealing ball, 41-lifting rod, 42-dewatering taper tube, 43-metal dome electrode, 44-cotton head, 45-waist-limit post, 46-47-hole, 48-support plate two.
Detailed Description
The technical scheme of the patent is further described in detail below with reference to the specific embodiments.
Embodiments of the present patent are described in detail below, examples of which are illustrated in the accompanying drawings, wherein the same or similar reference numerals refer to the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the drawings are exemplary only for explaining the present patent and are not to be construed as limiting the present patent.
Example 1:
1-12, a fully-automatic stem cell culture and expansion device based on micro-flow control comprises a base 3 and an incubator 1 for culturing cells, wherein the bottom of the incubator 1 is connected with a vibration seat 2 through a linkage oxygen supply piece 6, one end of the vibration seat 2 is rotatably connected with the base 3, the other end of the vibration seat 2 is connected with the base 3 through a vibration piece 5, the bottom of the incubator 1 is provided with a linkage oxygen supply piece 6, the vibration seat 2 is connected with the incubator 1 through a quick connecting piece 4, the linkage oxygen supply piece 6 comprises an aeration pipe 7 fixed inside the incubator 1 and a one-way valve 8 fixed on the outer wall of the bottom of the incubator 1, one end of the one-way valve 8 is connected with a cylinder body 11, the other end of the one-way valve 8 is connected with the aeration pipe 7 through a hose, the outer wall of the cylinder body 11 is fixedly provided with a one-way valve II 12 through a bolt, the inner wall of the cylinder body 11 is movably matched with a piston 9, the bottom of the piston 9 is rotatably connected with a piston rod 10, and the other end of the piston rod 10 is rotatably connected with the top outer wall of the base 3; when the incubator 1 vibrates to perform micro-flow control, the one-way valve 8 and the cylinder body 11 vibrate along with the micro-flow control, when the cylinder body 11 vibrates upwards, the piston 9 moves downwards, so that external air is sucked through the two one-way valves 12, then the one-way valve 8 vibrates downwards, and the sucked air is discharged into the incubator 1 through the one-way valve 8 and the aerator pipe 7, so that oxygen is supplied; the passage of the check valve 8 is from the cylinder 11 to the aeration pipe 7, and the passage of the second check valve 12 is from the outside to the inside of the cylinder 11.
The device can supply oxygen to the incubator 1 by arranging the linkage oxygen supply part 6, and the oxygen supply power and vibration are linked by the piston 9 and the piston rod 10, so that the oxygen supply rate and the vibration frequency are positively correlated, the single oxygen supply quantity is positively correlated with the vibration amplitude, the micro-flow control and the oxygen supply linkage in a vibration mode are realized, and the reliability of the oxygen supply is ensured.
To solve the problem of oxygen supply; as shown in fig. 3, the aeration pipe 7 comprises a transverse pipe 13 and a plurality of longitudinal pipes 15 communicated with the transverse pipe 13, wherein uniform aeration holes 14 are formed in the inner wall of one side of the longitudinal pipe 15, and the aeration holes 14 face to the bottom; this device through setting up a plurality of vertical tubes 15, it can guarantee to spread the bottom of incubator 1 to make oxygen supply region comparatively even, in addition, set up aeration hole 14 towards the bottom, in oxygen supply, also can float the nutrient solution precipitate of incubator 1 bottom, thereby play the function of preventing precipitation.
To solve the quick connection problem; as shown in fig. 4, the quick connector 4 includes a guide slide rail 17 fixed on the top outer wall of the vibration seat 2 by a bolt, and a guide slide block 16 fixed on the bottom outer wall of the incubator 1 by a bolt and slidably matched with the guide slide rail 17, wherein the inner wall of the guide slide rail 17 is slidably connected with a limit buckle 18, the bottom outer wall of the limit buckle 18 is welded with a first spring 19, and the inner wall of the guide slide block 16 is provided with a limit groove 20 matched with the limit buckle 18; during the use, the cooperation of accessible direction slider 16 and direction slide rail 17 slides incubator 1 to the top of vibrations seat 2, makes incubator 1 fixed relatively vibrations seat 2 through spacing knot 18 and spacing groove 20's mutual block to after the cultivation, can exert the effect to incubator 1 along direction rail 17 length direction, make spacing knot 18 shrink, accomplish the quick separation of incubator 1 and vibrations seat 2, thereby increased the convenience of use.
To solve the micro-flow control problem in the form of vibration; as shown in fig. 5, the vibration member 5 includes a second spring 24 welded to the outer wall of the opposite side of the vibration seat 2 and the base 3, and a cam 22 in contact with and matched with the outer wall of the bottom of the vibration seat 2, the cam 22 is rotatably connected to the top of the base 3 through a main shaft 21, a copper drum bracket on the outer wall of the top of the base 3 is connected with a motor 23, and the main shaft 21 is connected with an output shaft of the motor 23 through a coupling; when the motor 23 is started, the motor can drive the cam 22 to rotate through the main shaft 21, so that the vibration seat 2 vibrates under the action of the pulling force of the spring II 24, and the incubator 1 is driven to vibrate.
In order to solve the problem of amplitude and frequency adjustment; as shown in fig. 6 and 7, the cam 22 includes a wheel body 25 and adjustable protruding members 26 disposed on two sides of the wheel body 25, and two groups of the adjustable protruding members 26 are staggered.
The adjustable protruding rod piece 26 comprises a circular array type protruding rod 27 which is connected with the axial direction of the wheel body 25 in a sliding way, and a ball 28 which is connected with the end part of the protruding rod 27 in a rolling way; the vibration seat 2 is vibrated by the protrusion of the protruding rod 27, and the sliding of the protruding rod 27 changes the size of the protrusion of the protruding rod relative to the wheel body 25, thereby changing the amplitude.
An external thread boss 30 is welded on the outer wall of the wheel body 25, an adjusting disc 31 is movably sleeved on the outer wall of the external thread boss 30, a chute 32 is formed in the inner wall of the adjusting disc 31, and a protruding rod 27 is in limit fit with the inner wall of the chute 32 through a limit rod 29; when the adjustment disc 31 rotates, it can limit the change of the axial position of the protruding rod 27 by means of the chute 32.
The outer wall of the external thread boss 30 is connected with a pressure plate 33 through threads; after the position adjustment is completed, the pressing plate 33 may be screwed, which presses the adjusting plate 31, thereby fixing the position of the protruding rod 27.
The device can change the amplitude by arranging the adjustable protruding rod piece 26 and changing the protruding size of the protruding rod 27 in a mode of rotating the adjusting disc 31, and the two groups of adjustable protruding rod pieces 26 which are symmetrically arranged can also realize the switching of three modes of high-frequency single-amplitude vibration, low-frequency single-amplitude vibration and high-frequency double-amplitude vibration, so that the functionality of the device is improved.
In this embodiment, when in use, after the incubator 1 is fixed on the vibration seat 2 through the quick connector 4, stem cells and nutrient solution are input into the incubator 1, then vibration modes are selected according to the required modes, the states of the two groups of adjustable protruding rods 26 are adjusted, and then the motor 23 is started, so that vibration and oxygen supply can be performed.
Example 2:
a fully automatic stem cell culture and expansion device based on microflow control is shown in figure 1, in order to solve the problem of nutrient solution concentration; the present example was modified on the basis of example 1 as follows: it also comprises a concentration control part, which comprises a one-way sealing piece 34 arranged at the bottom of the incubator 1, a sampling piece 35 for sampling the concentration of the current nutrient solution and a sensing piece 36 for sensing the concentration.
The unidirectional sealing piece 34 comprises a filter plate 37 fixed on the inner wall of the bottom of the incubator 1 through bolts, a conical tube 39 welded on the outer wall of the bottom of the incubator 1 and a sealing ball 40 placed inside the conical tube 39, a spring III 38 is fixed on the outer wall of the opposite side of the filter plate 37 and the sealing ball 40, and the diameter of the sealing ball 40 is larger than the small opening diameter of the conical tube 39 and smaller than the large opening diameter of the conical tube 39.
The sampling member 35 includes a lifting rod 41 slidably coupled to the top of the base 3, and a cotton head 44 fixed to the top of the lifting rod 41 by bolts.
The sensing piece 36 comprises a water fading taper pipe 42 fixed on the outer wall of the top of the base 3 and metal spring plate electrodes 43 respectively fixed on two sides of the water fading taper pipe 42, wherein the two metal spring plate electrodes 43 are connected with the same sensing circuit, and the sensing circuit comprises a power supply and an electronic ammeter.
The concentration control part also comprises a high-concentration nutrient solution box and a power pump, wherein the inlet and the outlet of the power pump are respectively connected with the high-concentration nutrient solution box and the incubator 1, and the electronic ammeter is connected with the power pump through the upper computer.
The concentration control device is characterized by further comprising a linkage piece, wherein the linkage piece comprises a lever 47 and waist-shaped holes 46 formed in two ends of the lever 47, a second supporting plate 49 is fixed on the outer wall of the bottom of the incubator 1 through bolts, a first supporting plate 48 is fixed on the outer wall of the top of the base 3 through bolts, the second supporting plate 49 and the lifting rod 41 are respectively in limit fit with the waist-shaped holes 46 through limit posts 45, and the middle part of the limit posts 45 is rotationally connected with the first supporting plate 48.
When the incubator 1 vibrates downwards, the lifting rod 41 is driven to move upwards through the linkage action of the lever 47, so that the cotton head 44 is inserted into the bottom of the conical tube 39, the sealing ball 40 is jacked up, nutrient solution in the incubator 1 is absorbed by the cotton head 44, then the incubator 1 vibrates upwards, the sealing ball 40 is attached to the inner wall of the conical tube 39 by the elastic force of the spring III 38, self gravity and nutrient solution pressure, sealing is completed, the cotton head 44 contracts downwards, the metal spring plate electrodes 43 are communicated when the cotton head 44 contracts between the metal spring plate electrodes 43, an induction signal is generated by the electronic ammeter, when the current is smaller than a threshold value, the concentration of the reverse-pushing nutrient solution is reduced to the threshold value, the power pump is controlled to supplement, after induction, the cotton head 44 continues to contract until the inner diameter of the bottom of the water fading conical tube 42 is smaller, and the inductive nutrient solution is separated by the extrusion force.
The device is provided with the concentration control part, and the nutrient solution contains a plurality of inorganic salt components, so that the nutrient solution is electrolyte, can conduct electricity, has higher concentration and lower resistivity, and can monitor the concentration by monitoring the current by utilizing the characteristic, thereby realizing the guarantee of the concentration of the nutrient solution and improving the amplification efficiency.
In addition, through setting up cotton head 44 and taking a sample, it is through the vibrations motion of lever 47 linkage incubator 1 to realized automatic sampling, self-closing, auto-induction's function, increased the synchronism of device.
The foregoing is only a preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art, who is within the scope of the present invention, should make equivalent substitutions or modifications according to the technical scheme of the present invention and the inventive concept thereof, and should be covered by the scope of the present invention.
Claims (3)
1. The full-automatic stem cell culture and expansion device based on microflow control comprises a base (3) and an incubator (1) for culturing cells, and is characterized in that the bottom of the incubator (1) is connected with a vibration seat (2) through a linkage oxygen supply piece (6), one end of the vibration seat (2) is rotationally connected to the base (3), the other end of the vibration seat (2) is connected to the base (3) through a vibration piece (5), and the bottom of the incubator (1) is provided with the linkage oxygen supply piece (6);
the vibration seat (2) is connected to the incubator (1) through a quick connecting piece (4);
the linkage oxygen supply piece (6) comprises an aeration pipe (7) fixed in the incubator (1) and a one-way valve (8) fixed on the outer wall of the bottom of the incubator (1), one end of the one-way valve (8) is connected with a cylinder body (11), the other end of the one-way valve (8) is connected with the aeration pipe (7) through a hose, a one-way valve II (12) is fixedly arranged on the outer wall of the cylinder body (11), a piston (9) is movably matched with the inner wall of the cylinder body (11), a piston rod (10) is rotationally connected to the bottom of the piston (9), and the other end of the piston rod (10) is rotationally connected to the top outer wall of the base (3);
the vibration piece (5) comprises a spring II (24) fixedly arranged on the outer wall of one side opposite to the vibration seat (2) and the base (3) and a cam (22) in contact fit with the outer wall of the bottom of the vibration seat (2), the cam (22) is rotationally connected to the top of the base (3) through a main shaft (21), a copper drum bracket on the outer wall of the top of the base (3) is connected with a motor (23), and the main shaft (21) is connected with an output shaft of the motor (23) through a coupling;
the cam (22) comprises a wheel body (25) and adjustable protruding rods (26) arranged on two sides of the wheel body (25), and the two groups of adjustable protruding rods (26) are arranged in a staggered mode;
the adjustable protruding rod piece (26) comprises a circular array type protruding rod (27) which is connected with the axial direction of the wheel body (25) in a sliding mode and a ball (28) which is connected with the end portion of the protruding rod (27) in a rolling mode;
an outer wall of the wheel body (25) is fixedly provided with an outer thread boss (30), an outer wall of the outer thread boss (30) is movably sleeved with an adjusting disc (31), an inner wall of the adjusting disc (31) is provided with a chute (32), and a protruding rod (27) is in limit fit with the inner wall of the chute (32) through a limit rod (29);
the outer wall of the external thread boss (30) is connected with a pressure plate (33) through threads;
the concentration control part comprises a one-way sealing element (34) arranged at the bottom of the incubator (1), a sampling element (35) for sampling the concentration of the current nutrient solution and a sensing element (36) for sensing the concentration;
the unidirectional sealing piece (34) comprises a filter plate (37) fixedly arranged on the inner wall of the bottom of the incubator (1), a conical tube (39) fixedly arranged on the outer wall of the bottom of the incubator (1) and a sealing ball (40) arranged in the conical tube (39), and a spring III (38) is fixed on the outer wall of one side opposite to the filter plate (37) and the sealing ball (40);
the sampling piece (35) comprises a lifting rod (41) which is connected to the top of the base (3) in a sliding way, and a cotton head (44) which is fixedly arranged on the top of the lifting rod (41);
the induction piece (36) comprises a water fading taper pipe (42) fixed on the outer wall of the top of the base (3) and metal spring plate electrodes (43) fixed on two sides of the water fading taper pipe (42) respectively, wherein the two metal spring plate electrodes (43) are connected with the same induction circuit, and the induction circuit comprises a power supply and an electronic ammeter;
the concentration control part also comprises a high-concentration nutrient solution box and a power pump, wherein the inlet and the outlet of the power pump are respectively connected with the high-concentration nutrient solution box and the incubator (1), and the electronic ammeter is in control connection with the power pump through an upper computer;
the concentration control device is characterized by further comprising a linkage piece, wherein the linkage piece comprises a lever (47) and waist-shaped holes (46) formed in two ends of the lever (47), a second supporting plate (49) is fixedly arranged on the outer wall of the bottom of the incubator (1), a first supporting plate (48) is fixedly arranged on the outer wall of the top of the base (3), the second supporting plate (49) and the lifting rod (41) are respectively in limit fit with the waist-shaped holes (46) through limit columns (45), and the middle part of the limit columns (45) is rotationally connected to the first supporting plate (48).
2. The full-automatic stem cell culture expansion device based on micro-flow control according to claim 1, wherein the aeration pipe (7) comprises a transverse pipe (13) and a plurality of longitudinal pipes (15) communicated with the transverse pipe (13), one side inner wall of each longitudinal pipe (15) is provided with uniform aeration holes (14), and the aeration holes (14) face to the bottom.
3. The full-automatic stem cell culture expansion device based on the micro-flow control according to claim 1, wherein the quick connector (4) comprises a guide sliding rail (17) fixedly installed on the outer wall of the top of the vibration seat (2) and a guide sliding block (16) fixedly installed on the outer wall of the bottom of the incubator (1) and in sliding fit with the guide sliding rail (17), the inner wall of the guide sliding rail (17) is in sliding connection with a limit buckle (18), a first spring (19) is fixedly installed on the outer wall of the bottom of the limit buckle (18), and a limit groove (20) matched with the limit buckle (18) is formed in the inner wall of the guide sliding block (16).
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| CN102827769A (en) * | 2012-08-14 | 2012-12-19 | 中国科学院广州生物医药与健康研究院 | Automatic stem cell culture and amplification device based on microfluidics |
| CN216378239U (en) * | 2021-08-23 | 2022-04-26 | 邱斌 | Cell culture device for biotechnology |
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| JP5802751B2 (en) * | 2011-06-27 | 2015-11-04 | 株式会社日立製作所 | Cell culture device and cell culture method |
| WO2017107837A1 (en) * | 2015-12-23 | 2017-06-29 | 上海吉凯基因科技有限公司 | Fully automated continuous cell culture system |
| CN108485968B (en) * | 2018-04-28 | 2021-11-19 | 刘姣姣 | Novel cell stable culture equipment convenient to observe |
| CN208857306U (en) * | 2018-08-09 | 2019-05-14 | 卡替（上海）生物技术股份有限公司 | Expansion of stem cells device |
| CN112266874A (en) * | 2020-10-21 | 2021-01-26 | 李银玲 | Self-stabilization oxygenation type cell culture box |
| CN113136335A (en) * | 2021-04-27 | 2021-07-20 | 湖北明德健康科技有限公司 | Stem cell culture device and culture method thereof |
| CN113481098A (en) * | 2021-07-26 | 2021-10-08 | 南昌百特生物高新技术股份有限公司 | Full-automatic stem cell culture amplification device based on micro-fluidic |
| CN114107052B (en) * | 2021-12-03 | 2022-05-20 | 深圳市新一仑生物科技有限公司 | Portable stem cell amplification culture equipment capable of realizing online dynamic real-time monitoring |
| CN216378172U (en) * | 2021-12-17 | 2022-04-26 | 深圳市新一仑生物科技有限公司 | Bioreactor for stem cell exosome |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102827769A (en) * | 2012-08-14 | 2012-12-19 | 中国科学院广州生物医药与健康研究院 | Automatic stem cell culture and amplification device based on microfluidics |
| CN216378239U (en) * | 2021-08-23 | 2022-04-26 | 邱斌 | Cell culture device for biotechnology |
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