CN113136335A - Stem cell culture device and culture method thereof - Google Patents
Stem cell culture device and culture method thereof Download PDFInfo
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- CN113136335A CN113136335A CN202110458334.3A CN202110458334A CN113136335A CN 113136335 A CN113136335 A CN 113136335A CN 202110458334 A CN202110458334 A CN 202110458334A CN 113136335 A CN113136335 A CN 113136335A
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- 238000004113 cell culture Methods 0.000 title claims abstract description 42
- 238000012136 culture method Methods 0.000 title abstract description 6
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- 235000015097 nutrients Nutrition 0.000 claims abstract description 15
- 230000005540 biological transmission Effects 0.000 claims abstract description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 36
- 239000001301 oxygen Substances 0.000 claims description 36
- 229910052760 oxygen Inorganic materials 0.000 claims description 36
- 238000010438 heat treatment Methods 0.000 claims description 25
- 238000001914 filtration Methods 0.000 claims description 23
- 230000008602 contraction Effects 0.000 claims description 20
- 238000009423 ventilation Methods 0.000 claims description 14
- 238000001802 infusion Methods 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
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- 238000000034 method Methods 0.000 claims description 7
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- 102000004169 proteins and genes Human genes 0.000 claims description 5
- 108090000623 proteins and genes Proteins 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 3
- 239000000428 dust Substances 0.000 claims description 2
- 230000002093 peripheral effect Effects 0.000 claims description 2
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- 238000000429 assembly Methods 0.000 claims 3
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- 239000011247 coating layer Substances 0.000 claims 1
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- 210000004027 cell Anatomy 0.000 description 6
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- 238000012546 transfer Methods 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000003321 amplification Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
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- C12M23/10—Petri dish
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultraviolet radiation
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Abstract
The invention discloses a stem cell culture device and a culture method thereof, relating to the technical field of stem cell culture; aiming at solving the problem of low efficiency of adding nutrient solution; the device comprises a machine body, wherein a liquid box is arranged on the outer wall of the top of the machine body, a liquid conveying pipe is arranged on the outer wall of the top of the liquid box, a one-way valve is arranged on the outer wall of the circumference of the liquid conveying pipe, and the other end of the liquid conveying pipe penetrates through the outer wall of the top of the machine body; the outer wall of the top of the machine body is provided with an adjusting hand, the inner wall of the top of the machine body is provided with a rotating column, the input end of the rotating column is connected with the output end of the adjusting hand through a transmission shaft, and the outer wall of the circumference of the rotating column is provided with three connecting seats; the culture method of the device comprises the following steps: placing the culture dishes on the placing plate in sequence, and opening the ultraviolet lamp to continuously sterilize the organism for 30-100 min. The invention can quickly add nutrient solution without repeatedly opening the culture device, thereby reducing the hidden pollution danger, improving the culture yield and greatly saving the working time.
Description
Technical Field
The invention relates to the technical field of stem cell culture, in particular to a stem cell culture device and a culture method thereof.
Background
Stem cells are a type of pluripotent cells that have the ability to self-replicate. Under certain conditions, it can differentiate into a variety of functional cells. The stem cells are divided into embryonic stem cells and adult stem cells according to the development stage of the stem cells. When detecting stem cell, generally will cultivate stem cell, need carry out position shift to the incubator at the in-process of cultivateing stem cell usually, nevertheless often can arouse vibrations at the in-process that removes stem cell incubator, can arouse the culture solution of stem cell incubator inside to take place to rock when vibrations range is great, and stem cell can not arouse that the cell incubator rocks at the in-process of cultivateing, will arouse the death of cell like this, has increased the probability of cell death.
Through the retrieval, chinese patent application number is CN 202020330939.5's patent, discloses a neural stem cell culture device, including the incubator body, the sealed chamber door of hinge fixedly connected with is passed through in the front of incubator body, the front of sealed chamber door is provided with door handle, observation window and display respectively, the interior diapire of incubator body is provided with concave frid, the upper surface of concave frid is provided with pull formula culture tube support, the tube hole has been seted up to pull formula culture tube support's upper surface, the inner wall of tube hole is provided with neural stem cell culture tube, pull formula culture tube support's side fixedly connected with handle. The neural stem cell culture device in the above patent has the following disadvantages: in the long-time culture process, the culture tube needs to be opened repeatedly to inject the nutrient solution in sequence, the working efficiency is low, and the pollution risk is greatly increased.
Disclosure of Invention
The invention aims to solve the defects in the prior art and provides a stem cell culture device and a culture method thereof.
In order to achieve the purpose, the invention adopts the following technical scheme:
a stem cell culture device comprises a machine body, wherein a liquid box is arranged on the outer wall of the top of the machine body, a liquid conveying pipe is arranged on the outer wall of the top of the liquid box, a one-way valve is arranged on the outer wall of the circumference of the liquid conveying pipe, and the other end of the liquid conveying pipe penetrates through the outer wall of the top of the machine body; the top outer wall of organism is provided with the regulation hand, and the top inner wall of organism is provided with rotates the post, and the input that rotates the post is connected with the output of adjusting the hand through the transmission shaft, and the circumference outer wall that rotates the post is provided with three connecting seat, and the bottom inner wall of three connecting seat all is provided with the motor, and the top outer wall of three connecting seat all is provided with puts the dish, and the output of three motor is connected with the input of three putting the dish through the connecting axle respectively, and the top outer wall of three putting the dish all is provided with the reposition of redundant personnel structure.
Preferably: the top outer wall of organism is provided with perspective window, and the top outer wall of perspective window is provided with two mark circles, and the top outer wall of three arrangement dish all is provided with infrared lamp.
Preferably: the reposition of redundant personnel structure leads to groove, six guard plates and six ring channels including first logical groove, six second that collect liquid box, more than two, the top outer wall of safe dish has set gradually centre bore and six circular ports, the bottom outer wall of collection liquid box sets up in the bottom inner wall of centre bore, more than two first logical groove all sets up in the circumference outer wall of safe dish, six the second leads to the groove and all sets up in the circumference inner wall of centre bore, six the circumference outer wall of ring channel sets up respectively in the circumference inner wall of six circular ports, six the circumference outer wall of guard plate sets up respectively in the circumference inner wall that six circular ports are close to the ring channel.
Preferably: six the bottom outer wall of guard plate all is provided with the filter screen, and the circumference inner wall of six guard plates all coats and has the coating albumen layer, and the culture dish has all been placed to the bottom inner wall of six circular ports, and the top outer wall of six culture dishes all is provided with the lug more than two.
Preferably: the outer wall of one side of organism is provided with the chamber door, and the top outer wall of organism is provided with rubber component, and rubber component's inner wall is provided with the feed inlet, and the feed inlet includes that protective cover and sleeve pipe constitute.
Preferably: the relative both sides inner wall of organism all is provided with ultraviolet lamp, and one side inner wall of organism is provided with temperature sensor, and the inner wall all around of organism is provided with the heat preservation, and the three week inner walls that the heat preservation was hugged closely to the organism are provided with same heating chamber, and one side inner wall in heating chamber is provided with the heating rod, and one side outer wall of organism is provided with the inlet tube.
Preferably: the top outer wall of organism is provided with ventilative mouthful, and the top outer wall of ventilative mouthful is provided with first filtration piece, and first filtration piece is the slope form, and one side outer wall that ventilative mouthful is close to first filtration piece is provided with out the dirt mouth, and one side inner wall that ventilative mouthful is close to first filtration piece is provided with the second filtration piece, and one side inner wall that ventilative mouthful is close to the second filtration piece is provided with PTFE and filters the piece.
Preferably: one side outer wall of organism is provided with the mounting groove, one side outer wall of mounting groove is provided with the baffle, the bottom inner wall of mounting groove is provided with the air pump, there is shrink gasbag the output of air pump through pipe connection, one side inner wall of mounting groove is provided with the slide bar, the circumference outer wall sliding connection of slide bar has the push pedal, one side outer wall of push pedal sets up in shrink gasbag's one end, the oxygen package has been placed to the bottom inner wall of mounting groove, threaded connection is passed through in tracheal bottom to the output of oxygen package, tracheal top sets up in the top inner wall of mounting groove, and tracheal top is provided with the top cap.
Preferably: the bottom outer wall of organism is connected with same base through three snubber block, the top outer wall of three snubber block all sets up in the bottom outer wall of organism, the bottom inner wall of base is provided with the damper more than two, the inner wall all is provided with the extrusion piece around the base, the bottom outer wall of base is provided with four mounting grooves, the relative one side inner wall of four mounting grooves all is provided with the spout, the inner wall of two spouts is provided with same removal leg, the top outer wall of four removal legs all is provided with two elastic component, the top of two elastic component sets up in the top inner wall of same mounting groove.
A method for culturing a stem cell culture device comprises the following steps:
s1: placing the culture dishes on the placing plate in sequence, and opening the ultraviolet lamp to continuously sterilize the organism for 30-100 min;
s2: regulating the culture environment in the organism by the heating component;
s3: adjusting the positions of three connecting seats on the rotating column, and moving the stem cell culture body into a culture dish through a pipette when the ray emitted by an infrared lamp on the connecting seat under the feeding hole is aligned with a marking ring on the perspective window;
s4: when the ray emitted by the infrared lamp on the connecting seat under the infusion tube is aligned with the other marking ring on the perspective window, the nutrient solution is guided to the shunt structure from the infusion tube;
s5: controlling the supplementary gas of the contraction air bag to extrude the oxygen in the oxygen bag to be automatically ejected into the machine body;
s6: during the culture period, the gas inside and outside the organism is exchanged through the gas-permeable port.
The invention has the beneficial effects that:
1. when the culture solution is added, the one-way valve is opened, so that the culture solution in the solution box is guided into the shunting structure through the liquid conveying pipe, the nutrient solution is conveniently and quickly added, the culture device does not need to be repeatedly opened, the pollution hidden danger is reduced, the culture increase rate is improved, and the working time is greatly saved.
2. The stem cell culture medium is provided with the convex blocks and the coating protein layer, so that the residue of stem cells on the inner wall of the protective plate is reduced, the nutrition and respiratory metabolism of bottom cells can be obtained, the high-efficiency stem cell amplification can be realized conveniently, when the emission ray of the infrared lamp on the connecting seat under the feed port is aligned to the other marking ring on the transparent window through observation of the transparent window, the motor is closed, the pipette is inserted into the sleeve after the protective cover is opened, and the stem cell culture medium is moved into the culture dish for culture through the pipette, so that the operation is simple.
3. During the culture period, the air pump is started to supplement air into the contraction air bag according to specific use requirements, one end of the air pump extrudes the oxygen bag through the push plate along with the continuous expansion of the air in the contraction air bag, the oxygen in the oxygen bag is ejected along with the increase of the air pressure in the contraction air bag, the air pushes the top cover upwards to open, so that the oxygen is supplemented into the machine body, when the air pressure in the contraction air bag is insufficient, the oxygen in the oxygen bag is not enough to push the top cover, and the use is simple and convenient.
4. During the culture period, airflow of the machine body and the external environment is exchanged through the ventilation port to supplement clean air into the machine body, the production environment of stem cell culture is protected, the heat preservation layer prevents the temperature from being diffused too fast, repeated heating is caused, cost is increased, the culture solution flows into the liquid collection box, penetrates through the bottom of the central hole through more than two first through grooves, then is guided to flow into annular grooves in six circular holes through six second through grooves respectively, the culture solution flows into the bottom of the circular hole after circulating for a circle through the annular grooves, and flows into the culture dish through the filter screen along with the addition of the nutrient solution, so that stem cell bodies are soaked, and the efficient effect of adding the nutrient solution at one time is achieved.
5. When the device moves, if a certain moving leg meets a bulge, the bulge pushes the moving leg to compress the elastic component and move up and down in the chute, so that the overall vibration sense of the device during moving is further reduced, and the stability of the device during moving is improved.
Drawings
FIG. 1 is a schematic front view of a stem cell culture apparatus according to the present invention;
FIG. 2 is a schematic front view of a stem cell culture apparatus according to the present invention;
FIG. 3 is a schematic top view of a stem cell culture apparatus according to the present invention;
FIG. 4 is a schematic diagram of a side-view expanded structure of a stem cell culture apparatus according to the present invention;
FIG. 5 is a schematic view of a base structure of a stem cell culture apparatus according to the present invention;
FIG. 6 is a schematic diagram of a structure of a mounting plate of a stem cell culture apparatus according to the present invention;
FIG. 7 is a schematic side sectional view of a mounting plate of a stem cell culture apparatus according to the present invention;
FIG. 8 is a schematic diagram of an exploded structure of a mounting plate of a stem cell culture device according to the present invention;
FIG. 9 is a schematic circuit flow diagram of a stem cell culture apparatus according to the present invention.
In the figure: 1 machine body, 2 box doors, 3 bases, 4 adjusting hands, 5 infusion tubes, 6 liquid boxes, 7 temperature sensors, 8 rotating columns, 9 ultraviolet lamps, 10 heat preservation layers, 11 heating cavities, 12 top covers, 13 heating rods, 14 connecting seats, 15 perspective windows, 16 feeding holes, 17 rubber components, 18 first filtering components, 19 ventilation ports, 20 second filtering components, 21PTFE filtering components, 22 oxygen bags, 23 sliding rods, 24 baffle plates, 25 air pumps, 26 elastic components, 27 sliding grooves, 28 moving legs, 29 contraction air bags, 30 shock absorption blocks, 31 shock absorption components, 32 extrusion blocks, 33 arrangement discs, 34 liquid collection boxes, 35 infrared lamps, 36 protection plates, 37 bumps, 38 culture dishes, 39 motors, 40 second through grooves, 41 first through grooves, 42 annular grooves and 43 filter screens.
Detailed Description
The technical solution of the present patent will be described in further detail with reference to the following embodiments.
Reference will now be made in detail to embodiments of the present patent, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present patent and are not to be construed as limiting the present patent.
In the description of this patent, it is to be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the patent and for the simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the patent.
In the description of this patent, it is noted that unless otherwise specifically stated or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and can include, for example, fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meaning of the above terms in this patent may be understood by those of ordinary skill in the art as appropriate.
Example 1:
a stem cell culture device is shown in figures 1-3 and 6-8 and comprises a machine body 1, wherein a liquid box 6 is fixed on the outer wall of the top of the machine body 1 through bolts, a transfusion tube 5 is fixed on the outer wall of the top of the liquid box 6 through bolts, a one-way valve is fixed on the outer wall of the circumference of the transfusion tube 5 through bolts, and the other end of the transfusion tube 5 penetrates through the outer wall of the top of the machine body 1; the top outer wall of the machine body 1 is rotatably connected with an adjusting hand 4 through a rotating shaft, the top inner wall of the machine body 1 is rotatably connected with a rotating column 8 through the rotating shaft, the input end of the rotating column 8 is connected with the output end of the adjusting hand 4 through a transmission shaft, the circumferential outer wall of the rotating column 8 is fixedly provided with three connecting seats 14 through bolts, the bottom inner walls of the three connecting seats 14 are fixedly provided with motors 39 through bolts, the top outer walls of the three connecting seats 14 are rotatably connected with placing discs 33 through the rotating shaft, the output ends of the three motors 39 are respectively connected with the input ends of the three placing discs 33 through connecting shafts, and the top outer walls of the three placing discs 33 are provided with shunting structures; the top outer wall of organism 1 has perspective window 15 through the bolt fastening, the top outer wall bonding of perspective window 15 has two mark circles, the top outer wall of three arrangement dish 33 all is fixed with infrared lamp 35 through the bolt fastening, when needing to add the culture solution, manual rotation is adjusted hand 4 earlier and is made to rotate 8 rotations of post, and then adjust three connecting seat 14 positions on the rotation post 8, see through perspective window 15 and observe the connecting seat 14 point of whereabouts, when infrared lamp 35 on connecting seat 14 under transfer line 5 sent ray and aims at one of them mark circle on the perspective window 15, open check valve, make the culture solution in the liquid case 6 through transfer line 5 water conservancy diversion to the reposition of redundant personnel in the structure, and then be convenient for add the nutrient solution fast, need not to open culture apparatus repeatedly, reduce the pollution hidden danger, improve the cultivation increase rate.
To facilitate rapid addition of nutrient solution; as shown in fig. 6-8, the flow distribution structure includes a liquid collection box 34, more than two first through grooves 41, six second through grooves 40, six protection plates 36 and six annular grooves 42, the top outer wall of the placement plate 33 is sequentially provided with a central hole and six circular holes, the bottom outer wall of the liquid collection box 34 is fixed on the bottom inner wall of the central hole through bolts, more than two first through grooves 41 are all arranged on the circumferential outer wall of the placement plate 33, six second through grooves 40 are all arranged on the circumferential inner wall of the central hole, the circumferential outer walls of the six annular grooves 42 are respectively arranged on the circumferential inner walls of the six circular holes, the circumferential outer walls of the six protection plates 36 are respectively bonded on the circumferential inner walls of the six circular holes close to the annular grooves 42, the bottom outer walls of the six protection plates 36 are all fixed with filter screens 43 through bolts, and the circumferential inner walls of the six protection plates 36 are coated with coating protein layers, culture dish 38 has all been placed to the bottom inner wall of six circular ports, the top outer wall of six culture dishes 38 all bonds and has had two more than lugs 37, when 5 dropwise add culture solution from the transfer line, culture solution flows to in the collection liquid box 34, link up the centre bore bottom through two more than first logical groove 41, then in six annular groove 42 that lead respectively inflow six circular ports through six second logical grooves 40, the culture solution falls to the circular port bottom after 42 circulation of annular groove one week, along with the addition of nutrient solution, it passes in filter screen 43 inflow culture dish 38, thereby soak the stem cell body, lug 37 and encapsulated protein layer reduce the residue of stem cell at the 36 inner walls of guard plate, do benefit to the bottom cell and acquire nutrition and respiratory metabolism, be convenient for realize efficient stem cell amplification, disposable add the nutrient solution can, the operating time has been saved greatly.
To facilitate the addition of stem cell cultures; as shown in fig. 1-3 and fig. 9, the outer wall of one side of the machine body 1 is connected with a box door 2 through a hinge, the outer wall of the top of the machine body 1 is fixed with a rubber component 17 through a bolt, a feed inlet 16 is welded on the inner wall of the rubber component 17, the feed inlet 16 comprises a protective cover and a sleeve, the inner walls of two opposite sides of the machine body 1 are both fixed with ultraviolet lamps 9 through bolts, the inner wall of one side of the machine body 1 is fixed with a temperature sensor 7 through a bolt, the signal output end of the temperature sensor 7 is electrically connected with a control module, the type of the temperature sensor 7 is PT100, the inner walls around the machine body 1 are bonded with a heat insulating layer 10, the three inner walls of the machine body 1 tightly attached to the heat insulating layer 10 are provided with a same heating cavity 11, the inner wall of one side of the heating cavity 11 is fixed with a heating rod 13 through a bolt, the on-off control end of the heating rod 13 is electrically connected with the control module, the outer wall of one side of the machine body 1 is fixed with a water inlet pipe through a bolt, conveying water from a water inlet pipe into the heating cavity 11, opening the box door 2, sequentially placing the culture dishes 38 on the placing disc 33, closing the box door 2, opening the ultraviolet lamp 9 to continuously sterilize the machine body 1, driving the placing disc 33 to rotate by the starting motor 39, further conveniently adjusting the moving direction of the culture dishes 38, observing through the perspective window 15, when the infrared lamp 35 on the connecting seat 14 under the feeding hole 16 emits rays to be aligned with the other marking ring on the perspective window 15, closing the motor 39, inserting a pipette into the sleeve pipe after opening the protective cover, moving the dry cell culture body into the culture dishes 38 by the pipette for culture, starting the heating rod 13 during culture to rapidly heat the water in the heating cavity 11, monitoring the culture environment temperature in the machine body 1 in real time by the temperature sensor 7, transmitting a signal to the control module when the temperature is lower than or higher than a set value, and electrically connecting and controlling the start and stop of the heating rod 13, the insulating layer 10 prevents the temperature from being diffused too fast, resulting in repeated heating and increasing the cost.
In order to facilitate the supply of oxygen into the body 1; as shown in fig. 2-4, a ventilation port 19 is fixed on the outer wall of the top of the machine body 1 by a bolt, a first filtering member 18 is fixed on the outer wall of the top of the ventilation port 19 by a bolt, the first filtering member 18 is inclined, a dust outlet is formed on the outer wall of one side of the ventilation port 19 close to the first filtering member 18, a second filtering member 20 is fixed on the inner wall of one side of the ventilation port 19 close to the first filtering member 18 by a bolt, a PTFE filtering member 21 is fixed on the inner wall of one side of the ventilation port 19 close to the second filtering member 20 by a bolt, a mounting groove is formed on the outer wall of one side of the machine body 1, a baffle plate 24 is connected on the outer wall of one side of the mounting groove by a hinge, an air pump 25 is fixed on the inner wall of the bottom of the mounting groove by a bolt, the output end of the air pump 25 is connected with a contraction airbag 29 by a conduit, a sliding plate is fixed on the inner wall of one side of the mounting groove by a bolt 23, and a push plate is connected on the outer wall of the circumference of the sliding plate, the outer wall of one side of the push plate is bonded to one end of the contraction air bag 29, the inner wall of the bottom of the placing groove is provided with the oxygen bag 22, the output end of the oxygen bag 22 is connected to the bottom end of the air pipe through threads, the top end of the air pipe is fixed to the inner wall of the top of the placing groove through bolts, and the top end of the air pipe is connected with the top cover 12 through a hinge, when the oxygen bag is used, the oxygen bag 22 is placed in the placing groove, the output port of the oxygen bag 22 is fixed to the air pipe, the baffle 24 is closed, during the culture period, according to specific use requirements, the air pump 25 is started to supplement air into the contraction air bag 29, along with the continuous expansion of the air in the contraction air bag 29, one end of the oxygen bag 22 is extruded through the push plate, the oxygen in the oxygen bag 22 is ejected along with the increase of the air pressure in the contraction air bag 29, the air pushes the top cover 12 upwards to be opened, so as to supplement the oxygen into the machine body 1, when the air pressure in the contraction air bag 29 is insufficient, the oxygen in the oxygen bag 22 is not enough to push the top cover 12, convenient simple to use, during the cultivation, organism 1 and external environment's air current exchanges through ventilative mouthful 19, and when external air current got into organism 1 in, loop through first filter piece 18, second filter piece 20 and PTFE and filter piece 21 and filter the back, supply clean gas to organism 1 in, the production environment of protection stem cell cultivation.
In order to increase the smoothness of the equipment during movement; as shown in fig. 1, 4 and 5, the bottom outer wall of the machine body 1 is connected with the same base 3 through three shock-absorbing blocks 30, the top outer walls of the three shock-absorbing blocks 30 are all fixed on the bottom outer wall of the machine body 1 through bolts, the bottom inner wall of the base 3 is fixed with more than two shock-absorbing components 31 through bolts, the peripheral inner walls of the base 3 are all bonded with extrusion blocks 32, the bottom outer wall of the base 3 is provided with four mounting grooves, the inner wall of one side opposite to the four mounting grooves is provided with sliding grooves 27, the inner walls of the two sliding grooves 27 are connected with the same moving leg 28 through sliding blocks in a sliding manner, the top outer walls of the four moving legs 28 are all fixed with two elastic components 26 through bolts, the top ends of the two elastic components 26 are fixed on the top inner wall of the same mounting groove through bolts, the machine body 1 is pushed to move through the moving legs 28, the extrusion blocks 32 clamp the periphery of the machine body 1, vibration sense during the equipment removes is effectively slowed down to cooperation damper 31 and snubber block 30, avoids destroying the culture environment of stem cell, and when removing, certain removal leg 28 meets the arch on the way, and protruding top moves removal leg 28 makes its compression elastic component 26 and go up and down in spout 27, and whole vibration sense when further reducing the equipment and removing increases the stationarity when equipment removes.
In the embodiment, when the oxygen bag 22 is placed in the placing groove, after the outlet is fixed on the trachea, the baffle plate 24 is closed, water is delivered from the water inlet pipe to the heating chamber 11, the box door 2 is opened, the culture dishes 38 are sequentially placed on the placing disc 33 and then the box door 2 is closed, after the ultraviolet lamp 9 is opened to continuously sterilize the machine body 1, the adjusting hand 4 is manually rotated to rotate the rotating column 8, the positions of the three connecting seats 14 on the rotating column 8 are adjusted, the starting motor 39 drives the placing disc 33 to rotate, the moving direction of the culture dishes 38 is conveniently adjusted, the observation is carried out through the perspective window 15, when the infrared lamp 35 on the connecting seat 14 right below the feeding port 16 emits rays to align with the other marking ring on the perspective window 15, the motor 39 is closed, the pipette is inserted into the sleeve after the protective cover is opened, the dry cell culture is moved into the culture dish 38 through the pipette for culture, during the culture period, the heating rod 13 is started to enable the water in the heating cavity 11 to be heated up rapidly, the temperature sensor 7 monitors the culture environment temperature in the machine body 1 in real time, when the temperature is lower than or higher than a set value, a signal is transmitted to the control module, the heating rod 13 is electrically connected and controlled to be started and stopped, the heat preservation layer 10 avoids the rapid temperature dissipation, when the culture solution needs to be added, the point of the connecting seat 14 is observed through the perspective window 15, when the infrared lamp 35 on the connecting seat 14 under the infusion tube 5 sends rays to be aligned with one of the mark rings on the perspective window 15, the one-way valve is opened, so that the culture solution in the liquid box 6 is guided into the flow dividing structure through the infusion tube 5, the culture solution flows into the liquid collecting box 34, penetrates through the bottom of the central hole through more than two first through grooves 41, then is guided into the annular grooves 42 in six circular holes through six second through the annular grooves 40, and falls into the bottom of the circular hole after circulating for one circle, with the addition of nutrient solution, the nutrient solution flows into the culture dish 38 through the filter screen 43 to soak the stem cell bodies, the lug 37 and the coating protein layer reduce the residue of the stem cells on the inner wall of the protective plate 36, so as to be beneficial to obtaining nutrition and respiratory metabolism of the basal cell, during the culture period, according to the specific use requirements, the air pump 25 is started to supplement air into the contraction air bag 29, with the continuous expansion of the air in the contraction air bag 29, one end of the air pump presses the oxygen bag 22 through the push plate, the oxygen in the oxygen bag 22 is ejected out along with the increase of the air pressure in the contraction air bag 29, the air pushes the top cover 12 upwards to open, so as to supplement oxygen into the machine body 1, when the air pressure in the contraction air bag 29 is insufficient, the oxygen in the oxygen bag 22 is insufficient to push the top cover 12, during the culture period, the air flow of the machine body 1 and the external environment is exchanged through the ventilation port 19, and when the external air flow enters the machine body 1, the first filter 18, After the second filter element 20 and the PTFE filter element 21 are filtered, clean gas is supplied to the machine body 1, so as to protect the production environment of stem cell culture.
Example 2:
a method for culturing the stem cell culture device of example 1, as shown in FIG. 1, comprises the following steps:
s1: placing the culture dishes 38 on the placing plate 33 in sequence, and then opening the ultraviolet lamp 9 to continuously sterilize the organism 1 for 30-100 min;
s2: the culture environment in the body 1 is adjusted by the heating assembly;
s3: adjusting the positions of the three connecting seats 14 on the rotating column 8, and moving the stem cell culture body into a culture dish 38 through a pipette when an infrared lamp 35 on the connecting seat 14 just below the feeding hole 16 emits rays to be aligned with a mark ring on the perspective window 15;
s4: when the infrared lamp 35 on the connecting seat 14 under the infusion tube 5 emits rays to aim at another marking ring on the perspective window 15, the nutrient solution is guided to the shunt structure from the infusion tube 5;
s5: the supplementary gas of the contraction air bag 29 is controlled to extrude the oxygen in the oxygen bag 22 to be automatically ejected into the machine body 1;
s6: during the culture, the gas inside and outside the organism 1 is exchanged through the gas-permeable port 19.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (10)
1. A stem cell culture device comprises a machine body (1), and is characterized in that a liquid box (6) is arranged on the outer wall of the top of the machine body (1), a liquid conveying pipe (5) is arranged on the outer wall of the top of the liquid box (6), one-way valves are arranged on the outer wall of the circumference of the liquid conveying pipe (5), and the other end of the liquid conveying pipe (5) penetrates through the outer wall of the top of the machine body (1); the top outer wall of organism (1) is provided with adjusts hand (4), the top inner wall of organism (1) is provided with rotates post (8), the input of rotating post (8) is connected with the output of adjusting hand (4) through the transmission shaft, and the circumference outer wall of rotating post (8) is provided with three connecting seat (14), the bottom inner wall of three connecting seat (14) all is provided with motor (39), the top outer wall of three connecting seat (14) all is provided with puts dish (33), the output of three motor (39) is connected with the input of three dish (33) of putting through the connecting axle respectively, the top outer wall of three dish (33) of putting all is provided with the reposition of redundant personnel structure.
2. The stem cell culture device according to claim 1, wherein the top outer wall of the body (1) is provided with a transparent window (15), the top outer wall of the transparent window (15) is provided with two marker rings, and the top outer walls of the three mounting plates (33) are provided with infrared lamps (35).
3. A stem cell culture device according to claim 2, wherein the flow diversion structure comprises a drip box (34), more than two first through slots (41), six second through slots (40), six protection plates (36) and six annular grooves (42), the top outer wall of setting a set (33) has set gradually centre bore and six circular ports, the bottom outer wall of collection liquid box (34) sets up in the bottom inner wall of centre bore, more than two first logical groove (41) all set up in the circumference outer wall of setting a set (33), six the second leads to groove (40) and all sets up in the circumference inner wall of centre bore, six the circumference outer wall of ring channel (42) sets up respectively in the circumference inner wall of six circular ports, six the circumference outer wall of guard plate (36) sets up respectively in the circumference inner wall that six circular ports are close to ring channel (42).
4. The stem cell culture device according to claim 3, wherein the bottom outer walls of the six protection plates (36) are provided with filter screens (43), the circumferential inner walls of the six protection plates (36) are coated with protein coating layers, culture dishes (38) are placed on the bottom inner walls of the six circular holes, and the top outer walls of the six culture dishes (38) are provided with more than two lugs (37).
5. The stem cell culture device according to claim 4, wherein the outer wall of one side of the machine body (1) is provided with a box door (2), the outer wall of the top of the machine body (1) is provided with a rubber component (17), the inner wall of the rubber component (17) is provided with a feeding hole (16), and the feeding hole (16) comprises a protective cover and a sleeve.
6. The stem cell culture device according to claim 5, wherein the inner walls of two opposite sides of the machine body (1) are provided with ultraviolet lamps (9), the inner wall of one side of the machine body (1) is provided with a temperature sensor (7), the inner wall of the periphery of the machine body (1) is provided with a heat-insulating layer (10), the inner wall of three peripheries of the machine body (1) tightly attached to the heat-insulating layer (10) is provided with a same heating cavity (11), the inner wall of one side of the heating cavity (11) is provided with a heating rod (13), and the outer wall of one side of the machine body (1) is provided with a water inlet pipe.
7. The stem cell culture device according to claim 1, wherein the top outer wall of the machine body (1) is provided with a ventilation port (19), the top outer wall of the ventilation port (19) is provided with a first filtering piece (18), the first filtering piece (18) is inclined, the outer wall of the ventilation port (19) close to one side of the first filtering piece (18) is provided with a dust outlet, the inner wall of the ventilation port (19) close to one side of the first filtering piece (18) is provided with a second filtering piece (20), and the inner wall of the ventilation port (19) close to one side of the second filtering piece (20) is provided with a PTFE filtering piece (21).
8. The stem cell culture device according to claim 7, wherein a mounting groove is formed in the outer wall of one side of the machine body (1), a baffle (24) is arranged on the outer wall of one side of the mounting groove, an air pump (25) is arranged on the inner wall of the bottom of the mounting groove, the output end of the air pump (25) is connected with a contraction air bag (29) through a pipe, a sliding rod (23) is arranged on the inner wall of one side of the mounting groove, a push plate is connected to the outer wall of the circumference of the sliding rod (23) in a sliding manner, the outer wall of one side of the push plate is arranged at one end of the contraction air bag (29), an oxygen bag (22) is placed on the inner wall of the bottom of the mounting groove, the output end of the oxygen bag (22) is connected to the bottom end of the air pipe through threads, the top end of the air pipe is arranged on the inner wall of the top of the mounting groove, and the top end of the air pipe is provided with the top cover (12).
9. The stem cell culture device according to claim 8, wherein the same base (3) is connected to the bottom outer wall of the machine body (1) through three shock-absorbing blocks (30), the top outer walls of the three shock-absorbing blocks (30) are all arranged on the bottom outer wall of the machine body (1), the bottom inner wall of the base (3) is provided with more than two shock-absorbing assemblies (31), the peripheral inner walls of the base (3) are all provided with the extrusion blocks (32), the bottom outer wall of the base (3) is provided with four mounting grooves, the inner walls of the opposite sides of the four mounting grooves are all provided with the sliding grooves (27), the inner walls of the two sliding grooves (27) are provided with the same moving leg (28), the top outer walls of the four moving legs (28) are all provided with two elastic assemblies (26), and the top ends of the two elastic assemblies (26) are arranged on the top inner walls of the same mounting groove.
10. A method of culturing a stem cell culture device according to any one of claims 1 to 9, comprising the steps of:
s1: placing the culture dishes (38) on the placing plate (33) in sequence, and then turning on the ultraviolet lamp (9) to continuously sterilize the organism (1) for 30-100 min;
s2: the culture environment in the organism (1) is adjusted through the heating component;
s3: adjusting the positions of three connecting seats (14) on the rotating column (8), and when an infrared lamp (35) on the connecting seat (14) under the feeding hole (16) emits rays to be aligned with a mark ring on the perspective window (15), moving the stem cell culture body into a culture dish (38) through a pipette;
s4: when the infrared lamp (35) on the connecting seat (14) under the infusion tube (5) emits rays to be aligned with the other marking ring on the perspective window (15), the nutrient solution is guided to the shunt structure from the infusion tube (5);
s5: controlling the supplementary gas to the contraction air bag (29) to extrude the oxygen in the oxygen bag (22) to be automatically ejected into the machine body (1);
s6: during the culture period, the air inside and outside the organism (1) is exchanged through the ventilation port (19).
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| CN202110458334.3A CN113136335A (en) | 2021-04-27 | 2021-04-27 | Stem cell culture device and culture method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113913294A (en) * | 2021-10-08 | 2022-01-11 | 南京优而生物科技发展有限公司 | Method capable of inducing stem cells to secrete cytokines and culture device thereof |
| CN113999813A (en) * | 2021-10-12 | 2022-02-01 | 云南和泽西南生物科技有限公司 | Primary culture method of umbilical cord mesenchymal stem cells |
| CN115232747A (en) * | 2022-09-02 | 2022-10-25 | 湖北明德健康科技有限公司 | Full-automatic stem cell culture amplification device based on micro-flow control |
| CN115627232A (en) * | 2022-08-24 | 2023-01-20 | 曾峰 | Incubator for biological experiment cell culture |
| CN116855384A (en) * | 2023-08-28 | 2023-10-10 | 淄博市动物疫病预防与控制中心 | Riemerella anatipestifer phage culture device |
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2021
- 2021-04-27 CN CN202110458334.3A patent/CN113136335A/en not_active Withdrawn
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113913294A (en) * | 2021-10-08 | 2022-01-11 | 南京优而生物科技发展有限公司 | Method capable of inducing stem cells to secrete cytokines and culture device thereof |
| CN113999813A (en) * | 2021-10-12 | 2022-02-01 | 云南和泽西南生物科技有限公司 | Primary culture method of umbilical cord mesenchymal stem cells |
| CN115627232A (en) * | 2022-08-24 | 2023-01-20 | 曾峰 | Incubator for biological experiment cell culture |
| CN115232747A (en) * | 2022-09-02 | 2022-10-25 | 湖北明德健康科技有限公司 | Full-automatic stem cell culture amplification device based on micro-flow control |
| CN116855384A (en) * | 2023-08-28 | 2023-10-10 | 淄博市动物疫病预防与控制中心 | Riemerella anatipestifer phage culture device |
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Application publication date: 20210720 |