CN115227587B - Preparation method and application of hydrophilic astaxanthin emulsified and dispersed oil - Google Patents

Preparation method and application of hydrophilic astaxanthin emulsified and dispersed oil Download PDF

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CN115227587B
CN115227587B CN202210773805.4A CN202210773805A CN115227587B CN 115227587 B CN115227587 B CN 115227587B CN 202210773805 A CN202210773805 A CN 202210773805A CN 115227587 B CN115227587 B CN 115227587B
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astaxanthin
hydrophilic
oil
tween
preparation
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CN115227587A (en
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张勇
王天黎
郑云剑
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Elfa Biotechnology Jiaxing Co ltd
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Elfa Biotechnology Jiaxing Co ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/35—Ketones, e.g. benzophenone
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G9/00—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor
    • A23G9/32—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds
    • A23G9/36—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds containing microorganisms or enzymes; containing paramedical or dietetical agents, e.g. vitamins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23G—COCOA; COCOA PRODUCTS, e.g. CHOCOLATE; SUBSTITUTES FOR COCOA OR COCOA PRODUCTS; CONFECTIONERY; CHEWING GUM; ICE-CREAM; PREPARATION THEREOF
    • A23G9/00—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor
    • A23G9/32—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds
    • A23G9/42—Frozen sweets, e.g. ice confectionery, ice-cream; Mixtures therefor characterised by the composition containing organic or inorganic compounds containing plants or parts thereof, e.g. fruits, seeds, extracts
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L21/00—Marmalades, jams, jellies or the like; Products from apiculture; Preparation or treatment thereof
    • A23L21/10—Marmalades; Jams; Jellies; Other similar fruit or vegetable compositions; Simulated fruit products
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/03—Organic compounds
    • A23L29/035—Organic compounds containing oxygen as heteroatom
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/10—Foods or foodstuffs containing additives; Preparation or treatment thereof containing emulsifiers
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/105—Plant extracts, their artificial duplicates or their derivatives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
    • A61K8/04—Dispersions; Emulsions
    • A61K8/06—Emulsions
    • A61K8/066—Multiple emulsions, e.g. water-in-oil-in-water
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
    • A61K8/34—Alcohols
    • A61K8/345—Alcohols containing more than one hydroxy group
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/49—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing heterocyclic compounds
    • A61K8/4993—Derivatives containing from 2 to 10 oxyalkylene groups
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K8/00—Cosmetics or similar toiletry preparations
    • A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
    • A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
    • A61K8/55—Phosphorus compounds
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
    • A61Q19/00—Preparations for care of the skin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention discloses a preparation method and application of hydrophilic astaxanthin emulsified and dispersed oil, wherein the preparation method comprises the following preparation steps: step one, mixing tween and phospholipid, heating to 50-60 ℃, and stirring until the mixture is uniform to obtain a composite emulsifier; step two, mixing the composite emulsifying agent and propylene glycol, heating to 50-60 ℃, and stirring until the mixture is fully and uniformly mixed to obtain the composite emulsifying stabilizer; and thirdly, mixing the compound emulsion stabilizer with astaxanthin oil with the astaxanthin content of 10 percent, heating to 50-60 ℃, stirring until the mixture is fully and uniformly mixed, cooling to normal temperature, and homogenizing at high pressure to obtain the hydrophilic astaxanthin emulsion dispersion oil. The invention has the characteristics of strong hydrophilicity, high stability and wide application.

Description

Preparation method and application of hydrophilic astaxanthin emulsified and dispersed oil
Technical Field
The invention relates to astaxanthin emulsifying dispersion oil, in particular to a preparation method and application of hydrophilic astaxanthin emulsifying dispersion oil.
Background
The natural astaxanthin obtained from haematococcus pluvialis is orange-red ketone carotenoid, can inhibit lipid oxidation of food, effectively remove free radicals in vivo, and has various biological activities such as improving immunity of organism. Therefore, the astaxanthin extracted from haematococcus pluvialis has wide application prospect in the fields of foods, cosmetic skin care products and the like.
However, natural astaxanthin is a fat-soluble substance, has extremely poor solubility in a water-soluble system, and is limited in application form to capsules and powder. In addition, because astaxanthin is a long-chain unsaturated conjugated double bond structure system, the astaxanthin is unstable in property and easy to isomerize and degrade. Astaxanthin is sensitive to oxygen, sunlight and ultraviolet irradiation, temperature, and isomerization of astaxanthin is caused by conditions such as heat treatment and alkalinity, which greatly limit the wide application of astaxanthin. In order to enhance the stability of astaxanthin and increase the storage time of astaxanthin, it is common to prepare astaxanthin into emulsion. However, the oil phase in the emulsion is astaxanthin oil with relatively low astaxanthin content, usually only below 5%, but the astaxanthin oil with high astaxanthin content above 5% (the astaxanthin oil with high astaxanthin content is 10% in the astaxanthin oil extracted in the market at present) still cannot be well controlled in stability, and in the process of preparing the emulsion, other additives such as oil phase, ethanol and the like are added to influence the purity of the finished product, so that the application field is limited, and the hydrophilic astaxanthin emulsified dispersion oil with high astaxanthin content cannot be obtained and applied to various fields.
Disclosure of Invention
The invention aims to provide a preparation method and application of hydrophilic astaxanthin emulsified and dispersed oil. The invention has the characteristics of strong hydrophilicity, high stability and wide application.
The technical scheme of the invention is as follows: a preparation method of hydrophilic astaxanthin emulsified and dispersed oil comprises the following preparation steps:
step one, mixing tween and phospholipid, heating to 50-60 ℃, and stirring until the mixture is uniform to obtain a composite emulsifier;
step two, mixing the composite emulsifying agent and propylene glycol, heating to 50-60 ℃, and stirring until the mixture is fully and uniformly mixed to obtain the composite emulsifying stabilizer;
and thirdly, mixing the compound emulsion stabilizer with astaxanthin oil with the astaxanthin content of 10%, heating to 50-60 ℃, stirring to fully and uniformly mix, cooling to normal temperature, and carrying out superfine homogenization to nano-scale by a high-pressure homogenizer to obtain the hydrophilic astaxanthin emulsion dispersion oil.
In the preparation method of the hydrophilic astaxanthin emulsifying dispersion oil, the stirring time of the first step is 5-10 min, the stirring time of the second step is 2-5 min, the stirring time of the third step is 1-2 min, and the high-pressure superfine homogenization is carried out once.
In the preparation method of the hydrophilic astaxanthin emulsifying dispersion oil, in the first step, the mass ratio of tween to phospholipid is 3:1-6:1.
In the preparation method of the hydrophilic astaxanthin emulsifying dispersion oil, in the second step, the mass ratio of the composite emulsifier to the propylene glycol is 5:1-9:1.
In the preparation method of the hydrophilic astaxanthin emulsifying dispersion oil, in the third step, the mass ratio of the composite emulsion stabilizer to the astaxanthin oil is 3:1-9:1.
In the preparation method of the hydrophilic astaxanthin emulsifying dispersion oil, tween is an emulsifying agent of tween series, phospholipid is phosphatidylcholine or phospholipid with the content of phosphatidylcholine being 25-50%, and the purity of propylene glycol is more than 99%.
In the preparation method of the hydrophilic astaxanthin emulsifying dispersion oil, the tween adopts tween 80, tween 60, tween 40 or tween 20; the phospholipid is soybean phospholipid or lecithin.
In the preparation method of the hydrophilic astaxanthin emulsifying dispersion oil, the high-pressure homogenizing pressure in the third step is 15/65 MPpa-30/150 MPa.
The application of the hydrophilic astaxanthin emulsifying dispersion oil in preparing cosmetics, including emulsion, is also included.
The application of the hydrophilic astaxanthin emulsifying dispersion oil in preparing food is also included.
Compared with the prior art, the invention has the beneficial effects that:
the invention adopts a composite emulsifier composed of tween with hydrophile and lipophile and phospholipid, wherein the tween is more hydrophilic than lipophile, the phospholipid is less hydrophilic than lipophile, and the HLB (hydrophile-lipophile balance value) of the composite emulsifier obtained by a certain proportion reaches a stable system of 13.5-15, one side is hydrophilic group, and the other side is lipophilic group; the lipophilic group acts on the astaxanthin oil, the hydrophilic group acts on the propylene glycol, the hydrophilic group is arranged on the two-phase surface, stable protective interfacial activity is formed on the surface of the astaxanthin oil particles, and good protection is formed on the stability of the astaxanthin oil and the degradation of the astaxanthin oil.
The added propylene glycol can improve the fluidity of the composite emulsion stabilizer, reduce the viscosity of oil and reduce sediment.
The prepared hydrophilic astaxanthin emulsifying dispersion oil not only has high astaxanthin content, but also can ensure that the astaxanthin oil with high concentration is not degraded at room temperature for two years, and does not generate precipitation, layering and discoloration, the stability of the astaxanthin oil with high hydrophilicity is greatly enhanced and improved compared with that of the astaxanthin oil with low concentration, and the astaxanthin oil with high hydrophilicity is used as a functional ingredient, can be mixed with hydrophilic matrix materials at will according to the requirement and dissolved into the astaxanthin oil, widens the application field and range of the astaxanthin, such as the application in the fields of foods, including beverages, ice cream, jam and the like, cosmetics, skin care products and the like, and fully plays the functions of resisting oxidation and scavenging free radicals of the astaxanthin.
The whole preparation process is simple and feasible, is biological and environment-friendly, requires less time for each step, can be used for continuous production, and has high working efficiency.
Drawings
FIG. 1 is a graph showing the stability of the hydrophilic astaxanthin emulsion dispersion oil of example 2;
FIG. 2 is a graph showing particle diameters of the hydrophilic astaxanthin emulsifying dispersion oil of example 1;
FIG. 3 is a graph showing particle diameters of the hydrophilic astaxanthin emulsifying dispersion oil of example 2;
FIG. 4 is a graph showing particle diameters of the hydrophilic astaxanthin emulsifying dispersion oil of example 3;
FIG. 5 is a graph showing particle diameters of the hydrophilic astaxanthin emulsifying dispersion oil of example 4;
FIG. 6 is a graph of the particle size of the astaxanthin emulsion according to example 5.
Detailed Description
The invention is further illustrated by the following examples, which are not intended to be limiting.
A preparation method of hydrophilic astaxanthin emulsified and dispersed oil comprises the following preparation steps:
firstly, mixing tween and phospholipid according to a mass ratio of 3:1-6:1, heating in a water bath to 50-60 ℃, magnetically stirring for 5-10 min to fully and uniformly mix to obtain a composite emulsifier; the tween adopts tween-series emulsifying agent, and the phospholipid adopts phosphatidylcholine or phospholipid with the content of 25-50% of choline phospholipid;
mixing the composite emulsifying agent and propylene glycol according to the mass ratio of 5:1-9:1, heating to 50-60 ℃ in a water bath, and magnetically stirring for 2-5 min until the components are fully and uniformly mixed to obtain the composite emulsifying stabilizer; the purity of the propylene glycol is more than 99%;
mixing the composite emulsion stabilizer and astaxanthin oil with the astaxanthin content of 10% according to the mass ratio of 3:1-9:1, heating to 40-60 ℃ in a water bath, magnetically stirring for 1-2 min to fully and uniformly mix, cooling to normal temperature, carrying out high-pressure superfine homogenization for one time until the particles are below 400nm, and obtaining the hydrophilic astaxanthin emulsion dispersion oil with the homogenization pressure of 15/65 MPpa-30/150 MPa. The astaxanthin oil is extracted from haematococcus pluvialis.
Example 1:
a preparation method of hydrophilic astaxanthin emulsified and dispersed oil comprises the following preparation steps:
mixing tween 60 and phospholipid Pc25 according to a mass ratio of 5:1, heating to 50 ℃ in a water bath, magnetically stirring for 8min to fully dissolve, and uniformly mixing to obtain a composite emulsifier;
mixing the composite emulsifying agent and propylene glycol according to the mass ratio of 6:1, heating to 50 ℃ in a water bath, magnetically stirring for 3min to fully and uniformly mix to obtain the composite emulsifying stabilizer; the purity of the propylene glycol is more than 99%;
mixing the composite emulsion stabilizer and astaxanthin oil with the astaxanthin content of 10% according to the mass ratio of 7.3:1, heating to 45 ℃ in a water bath, magnetically stirring for 1min to fully and uniformly mix, cooling to normal temperature, carrying out high-pressure superfine homogenization for one time, and obtaining the hydrophilic astaxanthin emulsion dispersion oil with the high-pressure homogenization pressure of 15/75 MPa.
The content of astaxanthin oil in the hydrophilic astaxanthin emulsified dispersion oil was 12%.
Example 2:
a preparation method of hydrophilic astaxanthin emulsified and dispersed oil comprises the following preparation steps:
mixing Tween 80 and phospholipid Pc35 according to the mass ratio of 3.6:1, heating to 50 ℃ in a water bath, magnetically stirring for 8min to fully dissolve, and uniformly mixing to obtain a composite emulsifier;
mixing the composite emulsifying agent and propylene glycol according to the mass ratio of 5.3:1, heating to 50 ℃ in a water bath, and magnetically stirring for 3min to fully and uniformly mix to obtain the composite emulsion stabilizer; the purity of the propylene glycol is more than 99%;
mixing the composite emulsion stabilizer and astaxanthin oil with the astaxanthin content of 10% according to the mass ratio of 5.25:1, heating to 45 ℃ in a water bath, magnetically stirring for 1min to fully and uniformly mix, cooling to normal temperature, carrying out high-pressure superfine homogenization for one time, and obtaining the hydrophilic astaxanthin emulsion dispersion oil with the high-pressure homogenization pressure of 20/100 MPa.
The content of astaxanthin oil contained in the hydrophilic astaxanthin emulsion dispersion oil was 16%.
Example 3:
a preparation method of hydrophilic astaxanthin emulsified and dispersed oil comprises the following preparation steps:
mixing tween 40 and phospholipid Pc25 according to the mass ratio of 4.7:1, heating to 50 ℃ in a water bath, magnetically stirring for 8min to fully dissolve, and uniformly mixing to obtain a composite emulsifier;
mixing the composite emulsifying agent and propylene glycol according to the mass ratio of 6.8:1, heating to 50 ℃ in a water bath, and magnetically stirring for 3min to fully and uniformly mix to obtain the composite emulsion stabilizer; the purity of the propylene glycol is more than 99%;
mixing the composite emulsion stabilizer and astaxanthin oil with the astaxanthin content of 10% according to the mass ratio of 3.5:1, heating to 45 ℃ in a water bath, magnetically stirring for 1min to fully and uniformly mix, cooling to normal temperature, carrying out high-pressure superfine homogenization for one time, and obtaining the hydrophilic astaxanthin emulsion dispersion oil with the high-pressure homogenization pressure of 25/120 MPa.
The content of astaxanthin oil contained in the hydrophilic astaxanthin emulsion dispersion oil was 22%.
Example 4:
a preparation method of hydrophilic astaxanthin emulsified and dispersed oil comprises the following preparation steps:
mixing Tween 20 and phospholipid Pc45 according to a mass ratio of 5.7:1, heating to 50 ℃ in a water bath, magnetically stirring for 8min to fully dissolve, and uniformly mixing to obtain a composite emulsifier;
mixing the composite emulsifying agent and propylene glycol according to the mass ratio of 8.4:1, heating to 50 ℃ in a water bath, and magnetically stirring for 3min to fully and uniformly mix to obtain the composite emulsion stabilizer; the purity of the propylene glycol is more than 99%;
mixing the composite emulsion stabilizer and astaxanthin oil with the astaxanthin content of 10% according to the mass ratio of 3:1, heating to 45 ℃ in a water bath, magnetically stirring for 1min to fully and uniformly mix, cooling to normal temperature, carrying out high-pressure superfine homogenization for one time, and obtaining the hydrophilic astaxanthin emulsion dispersion oil with the high-pressure homogenization pressure of 25/145 MPa.
The content of astaxanthin oil in the hydrophilic astaxanthin emulsified dispersion oil was 25%.
Example 5:
the proposal also comprises the application of the astaxanthin oil in skin care products.
A preparation method of astaxanthin emulsion comprises the following steps:
the hydrophilic astaxanthin emulsified and dispersed oil prepared in the example 2 and deionized water are mixed according to the mass volume ratio of 0.4g:100ml of the water-soluble astaxanthin nano emulsion is mixed, stirred by magnetic force at normal temperature until the water-soluble astaxanthin nano emulsion is fully and uniformly mixed, and homogenized under high pressure of 10/30MPa by a homogenizer to obtain the stable, clear and transparent water-soluble astaxanthin nano emulsion. The water-soluble astaxanthin nano emulsion can be completely sieved by a 500-mesh sieve, and can not be layered, degraded and deteriorated within 2 years at normal temperature.
Example 6:
a preparation method of astaxanthin skin cream comprises the following steps:
at 40 ℃, the hydrophilic astaxanthin emulsifying and dispersing oil prepared in the example 1 is prepared according to the mass volume ratio of 1-1.2 g of hydrophilic astaxanthin emulsifying and dispersing oil: the proportion of 100mL skin cream is added into the skin cream, in particular to the skin cream which is prepared by the final preparation process of the skin cream, magnetically stirring and mixing evenly at normal temperature, and the skin cream with the astaxanthin strong antioxidant and sun-screening functions is prepared.
Example 7:
the proposal also comprises the application of the astaxanthin oil in food.
A preparation method of astaxanthin beverage comprises the following steps:
the hydrophilic astaxanthin emulsifying dispersion oil prepared in the example 3 is prepared according to the mass volume ratio of 0.6-0.8 g:100mL of the mixture is added into the dragon fruit juice milk beverage with the milk egg quality of 1.5 percent, and the uniform and stable dragon fruit juice milk beverage with the functions of scavenging free radicals and resisting oxidation is obtained after homogenization.
Example 8:
a preparation method of astaxanthin fruit and vegetable jam comprises the following steps:
the hydrophilic astaxanthin emulsifying dispersion oil prepared in the example 4 is prepared according to the mass volume ratio of 1-2 g:100mL of the astaxanthin-containing fruit and vegetable jam is added into the fruit and vegetable jam, and the fruit and vegetable jam with good stability and oxidation resistance is prepared by magnetic stirring and even mixing. The fruit and vegetable jam can be tomato jam, dragon jam, etc.
And (3) performance detection:
1. stability test of hydrophilic astaxanthin emulsified and dispersed oil:
the stability test was carried out at 60℃using the original astaxanthin oil extracted from astaxanthin extracted from Haematococcus pluvialis as a comparative example and the hydrophilic astaxanthin emulsion dispersion oil prepared in example 2 as an experimental example. FIG. 1 is a graph showing changes in astaxanthin concentration after storage at 60℃for various periods of time in comparative examples and experimental examples.
As can be seen from FIG. 1, the original astaxanthin oil has poor stability, and after 3 months at 60 ℃, the effective astaxanthin component is almost completely oxidized and decomposed, while the hydrophilic astaxanthin emulsified and dispersed oil can still basically maintain the original content and has high stability.
2. Particle size testing of examples 1-5;
the products of the examples were subjected to particle size testing using a Markov particle sizer, the results of which are shown in FIGS. 2-6.
The average particle diameter of the hydrophilic astaxanthin emulsified dispersion oil of example 1 was 161.1nm;
the average particle diameter of the hydrophilic astaxanthin emulsified dispersion oil of example 2 was 136.8nm;
the average particle diameter of the hydrophilic astaxanthin emulsified dispersion oil of example 3 was 338nm;
the average particle diameter of the hydrophilic astaxanthin emulsified dispersion oil of example 4 was 287nm;
the astaxanthin emulsion of example 5 had an average particle size of 217.7nm.
3. Transparency test of astaxanthin emulsion:
the transparency of the astaxanthin emulsion was measured using a transparency meter:
sample preparation astaxanthin emulsion was first filtered as a test sample with a 100 mesh copper screen and then placed in a 50mL beaker. Taking a self-contained vessel for containing the tested sample, cleaning the inside and the outside of the vessel by using a solvent corresponding to the tested sample, wiping the outside of the vessel by using self-contained lens paper of the instrument, spin-drying the inside of the vessel, and carefully pouring the prepared tested sample into the cleaned vessel. The liquid level is not lower than 4/5 of the vessel, so that the two transparent surfaces are ensured to have no stain for detection.
The detection method comprises the following steps: the instrument is placed horizontally, and the power supply is turned on to preheat the instrument. The upper lid of the upper measuring port of the instrument was covered, and the calibration instrument was carefully adjusted to give a display value of 100%. And opening an upper cover of the measuring port, inserting the prepared vessel, covering the upper cover, and reading the display percentage at the moment to obtain the transparency of the tested sample.
Detection result: the transparency of the astaxanthin emulsion is 99.6-99.8%. Almost equivalent to the transparency of purified water.

Claims (8)

1. A preparation method of hydrophilic astaxanthin emulsified and dispersed oil is characterized in that: the preparation method comprises the following preparation steps:
step one, mixing tween and phospholipid according to a mass ratio of 3:1-6:1, heating to 50-60 ℃, and stirring until the mixture is uniform to obtain a composite emulsifier;
mixing the composite emulsifying agent and propylene glycol according to the mass ratio of 5:1-9:1, heating to 50-60 ℃, and stirring to fully and uniformly mix to obtain the composite emulsion stabilizer;
and thirdly, mixing the compound emulsion stabilizer with astaxanthin oil with the astaxanthin content of 10%, heating to 50-60 ℃, stirring to fully and uniformly mix, cooling to normal temperature, and carrying out superfine homogenization to nano-scale by a high-pressure homogenizer to obtain the hydrophilic astaxanthin emulsion dispersion oil.
2. The method for preparing the hydrophilic astaxanthin emulsifying dispersion oil according to claim 1, wherein the method comprises the following steps: the stirring time of the first step is 5-10 min, the stirring time of the second step is 2-5 min, and the stirring time of the third step is 1-2 min.
3. The method for preparing the hydrophilic astaxanthin emulsifying dispersion oil according to claim 1, wherein the method comprises the following steps: in the third step, the mass ratio of the composite emulsion stabilizer to the astaxanthin oil is 3:1-9:1.
4. The method for preparing the hydrophilic astaxanthin emulsifying dispersion oil according to claim 1, wherein the method comprises the following steps: the tween adopts tween-series emulsifying agent, the phospholipid adopts phosphatidylcholine or phosphatidylcholine with content of 25-50%, and the purity of propylene glycol is above 99%.
5. The method for preparing the hydrophilic astaxanthin emulsifying dispersion oil according to claim 1, wherein the method comprises the following steps: the tween adopts tween 80, tween 60, tween 40 or tween 20; the phospholipid is soybean phospholipid or lecithin.
6. The method for preparing the hydrophilic astaxanthin emulsifying dispersion oil according to claim 1, wherein the method comprises the following steps: the high-pressure homogenizing pressure in the third step is 15/65 MPpa-30/150 MPa.
7. Use of a hydrophilic astaxanthin emulsifying dispersion oil according to any one of claims 1 to 6, characterized in that: the application of the hydrophilic astaxanthin emulsifying dispersion oil in preparing cosmetics comprises emulsion.
8. Use of a hydrophilic astaxanthin emulsifying dispersion oil according to any one of claims 1 to 6, characterized in that: the application of the hydrophilic astaxanthin emulsifying dispersion oil in preparing food.
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