CN115226632A - Cassava polyploid induction device and induction breeding method thereof - Google Patents
Cassava polyploid induction device and induction breeding method thereof Download PDFInfo
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- 208000020584 Polyploidy Diseases 0.000 title claims abstract description 48
- 240000003183 Manihot esculenta Species 0.000 title claims abstract description 44
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 title claims abstract description 44
- 238000009395 breeding Methods 0.000 title claims abstract description 22
- 230000006698 induction Effects 0.000 title claims description 20
- 239000001963 growth medium Substances 0.000 claims abstract description 37
- 239000000463 material Substances 0.000 claims abstract description 20
- 230000010355 oscillation Effects 0.000 claims abstract description 13
- 238000005520 cutting process Methods 0.000 claims abstract description 10
- 238000012216 screening Methods 0.000 claims abstract description 4
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 9
- 229960001338 colchicine Drugs 0.000 claims description 6
- 230000035755 proliferation Effects 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000005259 measurement Methods 0.000 claims description 5
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- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
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- 238000009630 liquid culture Methods 0.000 claims description 3
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- 238000000034 method Methods 0.000 abstract description 11
- 230000001488 breeding effect Effects 0.000 abstract description 9
- 230000001939 inductive effect Effects 0.000 abstract description 7
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/09—Apparatus for producing changes in chromosome number
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/002—Culture media for tissue culture
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Abstract
The invention discloses an inducing device and an inducing breeding method of cassava polyploidy in the technical field of cassava inducing breeding, wherein the inducing method comprises the following steps of firstly, taking aseptic cassava tissue culture seedlings as basic materials; step two, cutting the cassava tissue culture seedlings into single-bud stem sections, and transferring the single-bud stem sections into a culture medium A for oscillation treatment; transferring the cut single-bud stem segments into a culture medium B for cultivation, and separating ploidy chimeras; step four, performing ploidy identification on the whole leaf of the tissue culture seedling obtained by culture by adopting a flow cytometer, and identifying and screening polyploid pure line materials; and fifthly, expanding propagation, strengthening seedlings, rooting and transplanting survival of the cassava polyploid pure line material. The method has the advantages that the ploidy chimera is quickly separated through continuous multiple subcultures under the tissue culture condition, the separation time of the ploidy chimera is greatly shortened, the efficiency of quickly identifying the polyploidy by adopting a flow cytometer is high, and the breeding efficiency of the cassava polyploidy can also be improved.
Description
Technical Field
The invention belongs to the technical field of cassava induced breeding, and particularly relates to an induction device and an induction breeding method for cassava polyploids.
Background
Cassava is a plant of the genus cassava of the family euphorbiaceae, is drought-tolerant and barren-resistant, and is widely planted in the americas, asia, africa and other areas. The cassava root tuber has high edible value, is one of staple food in tropical and subtropical regions, can be used for squeezing alcohol and starch, and is an important industrial raw material. Cassava is one of the only important biomass energy crops developed in China, and is widely concerned by people. However, the existing cassava varieties are lack of high-quality varieties, the coverage rate of good varieties is low, and the requirements of domestic production development cannot be met, so that the breeding of the characteristic high-quality varieties with high and stable yield is very important. Polyploid breeding is one of the effective methods for improving plant character and breeding new variety with excellent economic character. After the plant is multiplied, the plant can be changed in aspects of phenotype, physiology, biochemistry and the like, such as thickening of leaves, deepening of colors such as corolla and leaf color, huge organ presentation of corolla, fruit and the like, vigorous growth of the plant, enhanced stress resistance and the like.
The existing breeding of cassava polyploidy is mostly cultivated under the field condition, colchicine is smeared on axillary buds of field plants to be used as an inducing material, the mode is inconvenient to obtain materials and is often influenced by environmental conditions, and the separation of ploidy chimera in the field can be completed within 2-3 years, which consumes a long time.
Disclosure of Invention
In order to solve the above problems, the present invention aims to set tissue culture conditions, and then to continuously subculture cassava for many times under the tissue culture conditions to rapidly isolate a ploidy chimera.
In order to achieve the purpose, the technical scheme of the invention is as follows: an induced breeding method of cassava polyploidy comprises the following steps,
taking a sterile cassava tissue culture seedling as a basic material for polyploidy induction,
step two, cutting the tissue culture seedling of the cassava into single bud stem sections with axillary buds, transferring the single bud stem sections into a culture medium A which is prepared in advance and sterilized, then placing a triangular flask containing the culture medium A into an induction device for oscillation treatment, taking out the single bud stem sections after oscillation is finished, then washing the single bud stem sections for 3 times by using sterile water, sucking water by using filter paper, cutting two sides of the stem sections,
step three, transferring the cut single-bud stem segments into a culture medium B for cultivation, carrying out continuous subculture for 4-6 times, separating ploidy chimeras,
step four, shearing the whole leaf of the tissue culture seedling obtained by 4-6 times of subculture, adopting a flow cytometer to carry out ploidy identification, identifying and screening polyploid pure line materials,
and step five, carrying out propagation on the polyploid pure line material through subculture proliferation, treating the material after the subculture proliferation culture, carrying out strong seedling rooting culture, moving the material into a greenhouse from a culture room after 30 days of rooting culture, hardening seedlings for 7 days, taking out the hardened tissue culture seedlings, cleaning, transplanting the seedlings into a culture medium, and ventilating and moisturizing the seedlings within 7 days after transplanting.
Further, no. 8 Huanan is selected as the aseptic cassava tissue culture seedling.
Furthermore, the stem section of the single bud is 2-3 cm long.
Further, the culture medium A is a liquid culture medium of MS + 0.075-0.1 mg/L colchicine, and the pH of the culture medium A is 5.8.
Further, the shaking treatment condition is that the shaking culture is carried out for 36 to 48 hours in the dark under the condition of 1000 to 2000r/min, and the culture temperature is 27 +/-1 ℃.
Further, the culture medium B is a semisolid culture medium of MS + 0.01-0.02 mg/L BA + 0.01-0.02 mg/L NAA +30g/L sucrose, the pH value of the culture medium B is 5.8, and the culture period is 40 days.
Further, the flow cytometer is used by taking 1cm 2 The measurement was performed by placing young leaves of a certain size in a small plastic dish, adding 0.5mL of a buffer solution, cutting the leaves with a single-sided blade, standing for 3min, adding about 0.5mL of a dye solution, and filtering the solution through a microporous filter into a 2.5mL small tube.
Further, an induction device for cassava polyploidy, the induction device comprises a box body, a rocking plate and a fixed plate are sequentially arranged in the box body from top to bottom, the fixed plate is fixedly connected with the bottom of the box body, a vertical plate is fixedly connected to one side of the fixed plate, a first rotating shaft is arranged in the vertical plate and extends to two sides of the vertical plate, a motor is arranged on the first rotating shaft, a motor output shaft is fixedly connected with the first rotating shaft coaxially, a second rotating shaft is fixedly connected with two ends of the first rotating shaft in an eccentric mode, a pulling rod is fixedly connected with the second rotating shaft and is far away from one side of the first rotating shaft, a vertical rod is fixedly connected with the top of the horizontal rod, the vertical rod is fixedly connected with the rocking plate, the rocking plate is provided with a first fixed block along the width direction, a fixed rod is fixedly connected with the first fixed block, a guide plate inclined is hinged to the fixed rod, one side, the side, of the guide plate, is hinged to a second fixed block, the top of the box body is hinged to be provided with a cover, a vent pipe communicated with the interior of the box body, a first check valve is arranged in the vent pipe, a temperature sensor and a controller are arranged on the box body, a support plate, a heater is arranged on one side, the support plate, the heater is connected with a blower through a connecting pipe, and communicated with a blower through a connecting pipe.
Furthermore, a plurality of partition plates are arranged on the rocking plate, the rocking plate is divided into a plurality of placing areas by the plurality of partition plates, a placing frame is arranged in the placing areas, and a clamping block is arranged in the placing frame.
After the scheme is adopted, the following beneficial effects are realized:
1. by adopting the cassava polyploidy induction method, the polyploidy chimera can be quickly separated through continuous and repeated subculture under the tissue culture condition, the polyploidy chimera is usually separated in 2-3 years under the field condition, the scheme can completely separate the polyploidy chimera within 1 year, the separation time of the polyploidy chimera is greatly shortened, the efficiency of quickly identifying the polyploidy by adopting a flow cytometer is high, the breeding efficiency of the cassava polyploidy is also improved, the axillary buds of tissue culture seedlings are convenient to obtain and are not limited by seasons and environmental conditions, the regeneration period of the induced plants is short, all conditions of the experiment can be strictly controlled on the basis of the tissue culture technology, and the operability and the repeatability of the experiment are strong.
2. Put into to placing in the frame the triangular flask that will be equipped with culture medium A, then close the lid, the temperature-sensing ware will be measured the temperature in the meeting box, convey the temperature that obtains to the controller with the measurement, the controller is after receiving temperature information, adjust according to required temperature, when the temperature is lower, open the second check valve, pour into steam into in the box, with temperature regulation, when steam pours into more, will produce vapor in the box, then under the control of controller, first check valve will be opened, make vapor pass through the breather pipe and discharge.
When the temperature in the box body is well regulated, the motor is started under the control of the controller, wherein the rotating speed of the motor is 1000-2000 r/min, the first rotating shaft can rotate under the driving of the motor, the pulling rod can be driven to transversely move through the second rotating shaft, the swinging plate can transversely swing within a certain range under the limitation of the guide plate under the driving of the pulling rod so as to simulate the oscillation operation, the triangular flask can swing under the driving of the swinging plate, the single-bud stem section can be fully contacted with colchicine solution so as to realize the cultivation of the single-bud stem section, a plurality of frames are placed on the swinging plate, the cultivation of a plurality of cultivation samples at the same time can be realized, the cultivation efficiency can be increased to a certain degree, the triangular flask can be stably fixed on the swinging plate under the fixing action of the fixture block, the displacement of the triangular flask during the oscillation treatment can be avoided, the cultivation of the single-bud stem section is influenced, and meanwhile, the required cultivation conditions in the box body can be flexibly regulated under the control of the controller, and the cultivation requirements can be more flexibly met.
Drawings
Fig. 1 is a front sectional view of an inducing apparatus for cassava polyploidy in an embodiment of the present invention.
Fig. 2 is a top view of a rocking plate of the inducing apparatus for cassava polyploidy in the embodiment of the present invention.
Detailed Description
The following is further detailed by way of specific embodiments:
reference numerals in the drawings of the specification include: the automatic lifting device comprises a box body 1, a cover 2, a vent pipe 3, a first one-way valve 4, a first rotating shaft 5, a second rotating shaft 6, a pulling rod 7, a vertical plate 8, a fixing plate 9, a second fixing block 10, a guide plate 11, a supporting plate 12, a heater 13, a blower 14, a second one-way valve 15, a cross rod 16, a vertical rod 17, a shaking plate 18, a first fixing block 19, a partition plate 20, a placing frame 21 and a clamping block 22.
The embodiment is basically as shown in the attached figure 1: an induced breeding method of cassava polyploids comprises the following steps,
taking No. 8 sterile cassava tissue culture seedlings in south China as a basic material for polyploidy induction.
And step two, cutting the cassava tissue culture seedlings into 2-3 cm long single-bud stem sections with axillary buds, transferring the single-bud stem sections into a pre-prepared and sterilized culture medium A, wherein the pH of the culture medium A is 5.8, the culture medium A is a liquid culture medium of MS + 0.075-0.1 mg/L colchicine, placing a triangular flask containing the culture medium A into an induction device for oscillation treatment, the oscillation treatment condition is that the culture medium A is subjected to dark oscillation culture for 36-48 h under the condition of 1000-2000 r/min, the culture temperature is 27 +/-1 ℃, taking out the culture medium after oscillation is finished, then washing the culture medium with sterile water for 3 times, absorbing water, and cutting two sides of the stem sections.
And step three, transferring the cut single-bud stem segments into a culture medium B for cultivation, wherein the pH value of the culture medium B is 5.8, the culture medium B is a semisolid culture medium of MS + 0.01-0.02 mg/L BA + 0.01-0.02 mg/L NAA +30g/L cane sugar, the cultivation period is 40 days, carrying out continuous subculture for 4-6 times, and separating ploidy chimeras.
Step four, shearing the whole leaf of the tissue culture seedling obtained by 4-6 times of subculture, adopting a flow cytometer to carry out ploidy identification, and identifying and screening the polyploid pure line material, wherein the using process of the flow cytometer is to take 1cm 2 The measurement was performed by placing young leaves of a certain size in a small plastic dish, adding 0.5mL of a buffer solution, cutting the leaves with a single-sided blade, standing for 3min, adding about 0.5mL of a dye solution, and filtering the solution through a microporous filter into a 2.5mL small tube.
And fifthly, carrying out propagation on the polyploid pure line material through subculture proliferation, treating the material after the subculture proliferation, carrying out strong seedling rooting culture, moving the material into a greenhouse for hardening seedlings for 7 days after rooting culture for 30 days, taking out the hardened tissue culture seedlings, cleaning, transplanting the tissue culture seedlings into a culture medium, and ventilating and moisturizing the tissue culture seedlings after transplanting for 7 days.
Has the advantages that: by adopting the cassava polyploidy induction method, the polyploidy chimera can be quickly separated through continuous and repeated subculture under the tissue culture condition, the polyploidy chimera is usually separated in 2-3 years under the field condition, the scheme can completely separate the polyploidy chimera within 1 year, the separation time of the polyploidy chimera is greatly shortened, the efficiency of quickly identifying the polyploidy by adopting a flow cytometer is high, the breeding efficiency of the cassava polyploidy is also improved, the axillary buds of tissue culture seedlings are convenient to obtain and are not limited by seasons and environmental conditions, the regeneration period of the induced plants is short, all conditions of the experiment can be strictly controlled on the basis of the tissue culture technology, and the operability and the repeatability of the experiment are strong.
The specific implementation process is as follows: put into to placing in the frame 21 the triangular flask that will be equipped with culture medium A, then close lid 2, the temperature-sensing ware will be measured meeting the temperature in the box 1, convey the temperature that obtains to the controller with the measurement, the controller is after receiving temperature information, adjust according to required temperature, when the temperature is lower, open second check valve 15, inject steam into in the box 1, with the regulation temperature, when steam injection is more, will produce vapor in the box 1, then under the control of controller, first check valve 4 will be opened, make vapor pass through breather pipe 3 and discharge.
After the temperature in the box body 1 is well regulated, the motor is started under the control of the controller, wherein the rotating speed of the motor is 1000-2000 r/min, the first rotating shaft 5 can rotate under the driving of the motor, then the pulling rod 7 can be driven to transversely move through the second rotating shaft 6, the swinging plate 18 can transversely swing within a certain range under the limitation of the guide plate 11 under the driving of the pulling rod 7 so as to simulate the oscillation operation, so that the triangular flask can swing under the driving of the swinging plate 18, the single-bud stem section and the colchicine solution can be relatively fully contacted, the single-bud stem section can be cultured, the culturing efficiency can be increased to a certain extent, the triangular flask can be relatively stably fixed on the swinging plate 18 under the fixing action of the clamping block 22, the displacement of the triangular flask during the oscillation treatment can be avoided, the single-bud stem section culturing is influenced, meanwhile, under the control of the controller, the required flexible regulation of the box body 1 can be more suitable for different culturing conditions.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.
The foregoing are embodiments of the present invention and are not intended to limit the scope of the invention to the particular forms set forth in the specification, which are set forth in the claims below, but rather are to be construed as the full breadth and scope of the claims, as defined by the appended claims, as defined in the appended claims, in order to provide a thorough understanding of the present invention. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several variations and modifications can be made, which should also be considered as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the utility of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (9)
1. An induced breeding method of cassava polyploids is characterized in that: comprises the following steps of (a) preparing a solution,
taking a sterile cassava tissue culture seedling as a basic material for polyploidy induction,
step two, cutting the tissue culture seedling of the cassava into single bud stem sections with axillary buds, transferring the single bud stem sections into a culture medium A which is prepared in advance and sterilized, then placing a triangular flask containing the culture medium A into an induction device for oscillation treatment, taking out the single bud stem sections after oscillation is finished, then washing the single bud stem sections for 3 times by using sterile water, sucking water by using filter paper, cutting two sides of the stem sections,
step three, transferring the cut single-bud stem segment into a culture medium B for cultivation, carrying out continuous subculture for 4-6 times, separating ploidy chimera,
step four, shearing the whole leaf of the tissue culture seedling obtained by 4-6 times of subculture, adopting a flow cytometer to carry out ploidy identification, identifying and screening polyploid pure line materials,
and fifthly, carrying out propagation on the polyploid pure line material through subculture proliferation, treating the material after the subculture proliferation, carrying out strong seedling rooting culture, moving the material into a greenhouse for hardening seedlings for 7 days after rooting culture for 30 days, taking out the hardened tissue culture seedlings, cleaning, transplanting the tissue culture seedlings into a culture medium, and ventilating and moisturizing the tissue culture seedlings after transplanting for 7 days.
2. The induced breeding method of cassava polyploids according to claim 1, characterized in that: the sterile cassava tissue culture seedling is No. 8 of south China.
3. The induced breeding method of cassava polyploids according to claim 2, characterized in that: the single bud stem is 2-3 cm long.
4. The induction breeding method of cassava polyploidy according to claim 3, which is characterized in that: the culture medium A is a liquid culture medium of MS + 0.075-0.1 mg/L colchicine, and the pH value of the culture medium A is 5.8.
5. The induced breeding method of cassava polyploids according to claim 4, wherein: the shaking treatment condition is that the shaking culture is carried out for 36 to 48 hours in the dark under the condition of 1000 to 2000r/min, and the culture temperature is 27 +/-1 ℃.
6. The induced breeding method of cassava polyploids according to claim 5, wherein: the culture medium B is a semisolid culture medium of MS + 0.01-0.02 mg/L BA + 0.01-0.02 mg/L NAA +30g/L sucrose, the pH value of the culture medium B is 5.8, and the culture period is 40 days.
7. The induced breeding method of cassava polyploids according to claim 6, wherein: the flow cytometer has been usedThe stroke is 1cm 2 The measurement was carried out by placing young leaves of a small size in a small plastic dish, adding 0.5mL of a buffer solution, cutting the leaves with a single-sided blade, standing for 3min, adding about 0.5mL of a dye solution, and filtering the solution through a microporous filter into a 2.5mL small tube.
8. An induction device for cassava polyploidy is characterized in that: the air blower comprises a box body, a rocking plate and a fixed plate are sequentially arranged in the box body from top to bottom, the fixed plate is fixedly connected with the bottom of the box body, a vertical plate is fixedly connected to one side of the fixed plate, a first rotating shaft is arranged in the vertical plate and extends to two sides of the vertical plate, a motor is arranged on the first rotating shaft, a motor output shaft is fixedly connected with the first rotating shaft coaxially, a second rotating shaft is fixedly connected with two ends of the first rotating shaft eccentrically, a pulling rod is fixedly connected with the second rotating shaft, the pulling rod is far away from a transverse rod fixedly connected with one side of the first rotating shaft, a vertical rod is fixedly connected with the top of the transverse rod, the vertical rod is fixedly connected with the rocking plate, the rocking plate is provided with a first fixed block along the width direction, a fixed rod is connected with the first fixed block, a guide plate inclined to the fixed rod is hinged to the guide plate, a second fixed block is hinged to one side of the guide plate, the fixed block is fixedly connected with the fixed plate, the top of the box body is hinged with a cover, a vent pipe is communicated with the interior of the box body, a first check valve is arranged in the vent pipe, a temperature sensor and a controller are arranged on the box body, a support plate, a heater is arranged on one side of the box body, and connected with a blower through a connecting wire.
9. The cassava polyploid induction apparatus according to claim 8, wherein: the swing plate is provided with a plurality of partition plates, the swing plate is divided into a plurality of placing areas by the partition plates, a placing frame is arranged in the placing area, and a clamping block is arranged in the placing frame.
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| CN104094853A (en) * | 2014-07-24 | 2014-10-15 | 广西壮族自治区农业科学院经济作物研究所 | Induction method of cassava polyploid |
| CN205435602U (en) * | 2015-11-30 | 2016-08-10 | 佛山金葵子植物营养有限公司 | Constant temperature vibrating machine |
| CN209276497U (en) * | 2018-10-23 | 2019-08-20 | 天津忆春生物科技有限公司 | A kind of constant-temperature shaking incubator |
| CN215029073U (en) * | 2021-05-27 | 2021-12-07 | 天津斯坦德优检测技术有限公司 | Temperature-controllable water bath shaking table |
| CN216367667U (en) * | 2021-12-08 | 2022-04-26 | 淮北师范大学 | Constant temperature shaking table with test tube fixing device |
| CN217221203U (en) * | 2021-12-02 | 2022-08-19 | 北京志超伟业生物技术有限公司 | Shaking table for uniformly mixing test tubes |
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| CN205435602U (en) * | 2015-11-30 | 2016-08-10 | 佛山金葵子植物营养有限公司 | Constant temperature vibrating machine |
| CN209276497U (en) * | 2018-10-23 | 2019-08-20 | 天津忆春生物科技有限公司 | A kind of constant-temperature shaking incubator |
| CN215029073U (en) * | 2021-05-27 | 2021-12-07 | 天津斯坦德优检测技术有限公司 | Temperature-controllable water bath shaking table |
| CN217221203U (en) * | 2021-12-02 | 2022-08-19 | 北京志超伟业生物技术有限公司 | Shaking table for uniformly mixing test tubes |
| CN216367667U (en) * | 2021-12-08 | 2022-04-26 | 淮北师范大学 | Constant temperature shaking table with test tube fixing device |
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