CN115216400B - Nucleic acid extraction detection control system and control method - Google Patents
Nucleic acid extraction detection control system and control method Download PDFInfo
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- CN115216400B CN115216400B CN202211133884.9A CN202211133884A CN115216400B CN 115216400 B CN115216400 B CN 115216400B CN 202211133884 A CN202211133884 A CN 202211133884A CN 115216400 B CN115216400 B CN 115216400B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/686—Polymerase chain reaction [PCR]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/02—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
- A61L2/08—Radiation
- A61L2/10—Ultra-violet radiation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q3/00—Condition responsive control processes
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
Abstract
A control system and method for extracting and detecting nucleic acid features that the real-time function and efficiency can reach a correct level, and the matrix reduction mode is used to obtain the value of signal frame, so obtaining some digital signals can be processed initially. The control of the LED illuminating lamp and the ultraviolet lamp is achieved through combining the interface on the touch screen, so that the efficiency is improved, and the control is accurate.
Description
Technical Field
The invention belongs to the technical field of extraction control, and particularly relates to a nucleic acid extraction detection control system and a control method.
Background
The nucleic acid extraction detection control system is a traditional nucleic acid extraction purification instrument, the nucleic acid extraction detection control system generally adopts the reaction of nucleic acid in a PCR tube, a laser emitter irradiates laser towards the PCR tube, a fluorescent signal detection piece collects fluorescent signals in the PCR tube, the fluorescent signals are transmitted to a controller and then transmitted to a background device for display, and the traditional nucleic acid extraction detection control system can only receive fewer channels for transmitting the fluorescent signals at the same time. With the development of software radio technology, high-speed digital sampling and signal processing technology is utilized, so that simultaneous receiving of multiple channels can be realized, and higher receiving sensitivity can be achieved.
The nucleic acid extraction detection control system comprises a nucleic acid extraction detection control system main body of the nucleic acid extraction detection control system, the nucleic acid extraction detection control system main body is arranged in the shell and comprises a plurality of paths of fluorescent signal acquisition channels, each path of fluorescent signal acquisition channel is uniformly connected with a controller, the controllers are also connected with the mobile communication module, and the controllers are connected with background devices in the mobile communication network through the mobile communication module.
Thus, the fluorescence signal acquisition channel is used for accessing the source fluorescence signal and outputting a fluorescence digital signal towards the controller; the controller is used for executing treatment on the fluorescence digital signal and storing the fluorescence digital signal in an external memory connected with the controller; the controller also frames the fluorescent digital signal into signal frames to perform the transfer; the background device receives the signal frame; the background device is used for analyzing and extracting the received signal frames to obtain fluorescence digital signals; the background device also stores the fluorescent digital signal; the fluorescence digital signal transmitted by the existing nucleic acid extraction detection control system has low efficiency on the real-time function and speed, and is not beneficial to the occasions where the fluorescence digital signal with high real-time function and speed efficiency is transmitted.
In addition, in the current nucleic acid extraction detection control system, an LED illuminating lamp and an ultraviolet lamp device for disinfection are often arranged, the control of the LED illuminating lamp and the ultraviolet lamp is controlled by manually pressing hardware keys, and the efficiency is low and the control is not accurate enough due to the fact that soft control is unavailable.
Disclosure of Invention
To solve the above-mentioned problems, it is an object of the present invention to provide a control system and method for nucleic acid extraction detection, which can achieve a good level of real-time performance and efficiency, and use a matrix reduction mode to obtain the value of a signal frame, so that the digital signals can be initially processed when they are obtained, and to ensure the real-time performance, the digital signals are simultaneously matrixed and various transformations are performed, and the initial digital signal is extremely reduced in capacity to perform transmission, thereby ensuring the speed and efficiency. The control of the LED illuminating lamp and the ultraviolet lamp is achieved through combining the interface on the touch screen, so that the efficiency is improved, and the control is accurate.
The invention adopts the following technical scheme.
A nucleic acid extraction detection control system comprising:
a nucleic acid extraction detection control system main body of the nucleic acid extraction detection control system and a shell of the nucleic acid extraction detection control system, wherein the nucleic acid extraction detection control system main body is arranged in the shell of the nucleic acid extraction detection control system;
the nucleic acid extraction detection control system main body comprises a plurality of paths of fluorescent signal acquisition channels, and each path of fluorescent signal acquisition channel is uniformly connected with a controller; the controller is also connected with the mobile communication module and the external memory, and the controller is connected with a background device in the mobile communication network through the mobile communication module; the fluorescence signal acquisition channel comprises a laser emitter, a fluorescence signal detection piece and a PCR tube containing nucleic acid to be detected, wherein the laser emitter, the fluorescence signal detection piece and the PCR tube are arranged in a shell of the nucleic acid extraction detection control system;
each path of the fluorescence signal acquisition channel is used for accessing a source fluorescence signal and outputting a fluorescence digital signal to a controller;
the components running on the controller comprise a signal receiving component, a signal preprocessing component, a signal transmission component and a nucleic acid extraction detection control system operating component, wherein the signal receiving component is used for receiving fluorescent signals transmitted by a fluorescent signal acquisition channel, the signal preprocessing component is used for performing framing processing on the fluorescent digital signals to obtain signal frames, the signal transmission component transmits the signal frames to the background device through the mobile communication module, the background device restores the signal frames to initial fluorescent digital signals and then performs analysis, the nucleic acid extraction detection control system operating component is used for controlling a receiving mode of the signal receiving component and a processing mode of the signal preprocessing component, and indexes confirming the receiving mode and the processing mode are transmitted to the nucleic acid extraction detection control system operating component through the background device and the mobile communication module.
A control method of a nucleic acid extraction detection control system is characterized in that a signal pre-processing unit comprises a temporary storage queue, the temporary storage queue is used for receiving and storing digital signals received by a signal receiving unit, the temporary storage queue performs cutting on the temporary storage queue according to a specification index transmitted by a background device, and the cutting comprises the following processes:
step 1: marking the appointed pointer with a mark according to the standard index, wherein the pointer with the mark forms a storage block, and the capacity of the storage block is confirmed by the standard index;
step 2: the method comprises the steps that a fluorescent digital signal to be stored is determined according to a standard index, if the fluorescent digital signal to be stored and the fluorescent digital signal stored in a storage block belong to an integral digital signal, the digital signal is stored in the storage block, if the digital signal to be stored and the digital signal stored in the storage block belong to a digital signal of a different integral, another storage block is selected from pointers which are not marked to be marked with marks, and the digital signal is stored in a new storage block, wherein the different storage block is provided with different marks;
and step 3: if the digital signal stored in the storage block is full, the pointer which is not marked is added into the storage block, and if the digital signal in the storage block is integrated, the pointer which is not used in the storage block is marked off.
The invention has the advantages that compared with the prior art, the invention can reach a good level of real-time function and efficiency, and the value of the signal frame is obtained by using the mode of square matrix reduction, so that the digital signal can be initially processed when some digital signals are obtained, the digital signals are synchronously matrixed and various conversions are executed for guaranteeing the real-time function, and the transmission is executed after the initial digital signal capacity is extremely reduced, thereby guaranteeing the speed and the efficiency. The control of the LED illuminating lamp and the ultraviolet lamp is achieved through combining the interface on the touch screen, so that the efficiency is improved, and the control is accurate.
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FIG. 1 is a block diagram of a portion of the components of the present invention;
FIG. 2 is a flow chart of steps 1 to 3 in the present invention.
Detailed Description
Some technical terms of the present invention have the following meanings:
the present application is further described below with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present application is not limited thereby.
The invention provides a nucleic acid extraction detection control system, which comprises:
a nucleic acid extraction detection control system main body of the nucleic acid extraction detection control system and a shell of the nucleic acid extraction detection control system, wherein the nucleic acid extraction detection control system main body is arranged in the shell of the nucleic acid extraction detection control system; a nucleic acid extraction detection control system, namely a nucleic acid extraction and purification instrument.
The nucleic acid extraction detection control system comprises a nucleic acid extraction detection control system main body and a controller, wherein the nucleic acid extraction detection control system main body comprises a plurality of paths of fluorescent signal acquisition channels, each path of fluorescent signal acquisition channel is uniformly connected with the controller, each fluorescent signal acquisition channel comprises a laser emitter, a fluorescent signal detection piece and a PCR tube containing nucleic acid to be detected, the laser emitter is arranged beside the PCR tube, the fluorescent signal detection piece is arranged in the PCR tube, so that the laser emitter irradiates laser towards the PCR tube, the fluorescent signal detection piece collects the fluorescent signal in the PCR tube, and the fluorescent signal acquisition channels can be connected with source fluorescent signals and output fluorescent digital signals towards the controller; the controller is also connected with the mobile communication module and the external memory, and is connected with a background device in the mobile communication network through the mobile communication module; the mobile communication module can be a 4G module, the mobile communication network can be a 4G network, and the background device can be a monitoring computer.
Each fluorescence signal acquisition channel is used for accessing a source fluorescence signal and outputting a fluorescence digital signal to a controller;
as shown in fig. 1, the components running on the controller include a signal receiving component, a signal pre-processing component, a signal transmitting component and a nucleic acid extraction and detection control system operating component, the signal receiving component is configured to receive a fluorescent signal transmitted by a fluorescent signal acquisition channel, the signal pre-processing component is configured to perform framing processing on a fluorescent digital signal to obtain a signal frame, the signal transmitting component transmits the signal frame to the background device via a mobile communication module, the background device recovers the signal frame into an initial fluorescent digital signal and then performs parsing, the nucleic acid extraction and detection control system operating component is configured to operate a receiving mode of the signal receiving component and a processing mode of the signal pre-processing component, and an index confirming the receiving mode and the processing mode is transmitted to the nucleic acid extraction and detection control system operating component via the mobile communication module via the background device.
In a preferred but non-limiting embodiment of the present invention, the signal pre-processing component divides the received fluorescent digital signal into a plurality of digital signals which are individually treated as a whole according to the index transmitted by the background device, and then processes each digital signal framing which is individually treated as a whole into a plurality of signal frames, wherein each signal frame includes an identifier, a digital signal value and a terminator, the identifier is used for representing the identifier of the digital signal which is individually treated as a whole and the identifier is located in the signal frame, the identifier is used for representing the orientation of the signal frame in the signal frame below the identifier, the digital signal value is the actual value of the corresponding digital signal, and the terminator is used for representing whether the signal frame is the last signal frame below the identifier.
In a preferred but non-limiting embodiment of the invention, the digital signal corresponding to a signal frame is the digital signal pointed by an array of pointers, represented by the matrix H:
here, x ef Representing the digital signal value pointed by a corresponding pointer and used as a component in the matrix, r is the rank of the pointer of the obtained square matrix, the categories of e and f are positive integers from 1 to r, and r is a positive integer;
said signalThe pre-treatment component is passed through the index l uv Treating the matrix H as an equivalent matrix I:
here, d ef Is a component within matrix I;
under the condition that e is equal to 1, d ef =x ef On condition that e is higher than f, d ef A true value of 0;
the index l uv The following conditions are met:
here, u and v are both the index l uv U is a positive integer from 1 to r-1, v is a positive integer from 1 to u +1, g is a positive integer from 2 to r, c is a positive integer from 1 to g, and g and c are used to represent the actual amounts of u and v, respectively;
the pre-signal processing component combines the component with non-0 value in the matrix I with the index l uv As values within a signal frame to the background device.
In a preferred but non-limiting embodiment of the present invention, the pre-signal processing unit includes a temporary storage queue, the temporary storage queue is divided into two or more storage blocks, one storage block is used for storing a digital signal which is used as a whole, pointers of one storage block are provided with same marks, pointers of different storage blocks are provided with different marks, and the pre-signal processing unit obtains the stored digital signal according to the marks of the pointers and forms a signal frame.
In a preferred but non-limiting embodiment of the invention, the terminator has two types, namely a normal terminator and an abort terminator, wherein the abort terminator represents that the signal frame is the last signal frame under an identifier, and the terminator of another signal frame is a normal terminator, and the abort terminator also has the value of the highest row number and the highest column number in the azimuth symbol.
In a preferred but non-limiting embodiment of the present invention, the background device performs grouping according to the identifier of the received signal frame, performs arrangement according to the direction symbol of the signal frame, runs a timer after receiving the signal frame with the abort symbol, confirms whether to receive the whole signal frame under the identifier in the time length set by the timer, and if there is a missing signal frame after the time length timed by the timer exceeds the set time length, transmits the corresponding identifier and direction symbol to the pre-signal processing unit through the mobile communication module, and requires re-transmission of the missing signal frame.
In a preferred but non-limiting embodiment of the present invention, after the background device receives all the signal frames under an identifier, the background device transmits the identifier and the confirmation message to the pre-signal processing unit via the mobile communication module, and the temporary storage queue in the pre-signal processing unit synchronously removes the mark of the corresponding pointer according to the zero resetting of the digital signal in the storage block of the corresponding identifier in the confirmation message.
In a preferred but non-limiting embodiment of the present invention, a touch screen is embedded on the outer wall of the casing of the nucleic acid extraction and detection control system, the controller is electrically connected with the touch screen, and an LED illuminating lamp and an ultraviolet lamp for disinfection, both of which are connected with the controller, are further arranged in the casing of the nucleic acid extraction and detection control system.
In the control method of the nucleic acid extraction detection control system according to the present invention, the signal pre-processing unit includes a temporary storage queue, the temporary storage queue is used for receiving and storing the digital signal received by the signal receiving unit, and the temporary storage queue performs a cutting operation on the temporary storage queue according to the specification index transmitted by the background device, as shown in fig. 2, the cutting operation includes the following steps:
step 1: marking the appointed pointer with a mark according to the standard index, wherein the pointer with the mark forms a storage block, and the capacity of the storage block is confirmed by the standard index;
step 2: the method comprises the steps that a fluorescent digital signal to be stored is determined according to a standard index, if the fluorescent digital signal to be stored and the fluorescent digital signal stored in a storage block belong to an integral digital signal, the digital signal is stored in the storage block, if the digital signal to be stored and the digital signal stored in the storage block belong to a digital signal of a different integral, another storage block is selected from pointers which are not marked to be marked with marks, and the digital signal is stored in a new storage block, wherein the different storage block is provided with different marks;
and step 3: if the digital signal stored in the storage block is full, the pointer which is not marked is added into the storage block, and if the digital signal in the storage block is integrated, the pointer which is not used in the storage block is marked off.
In a preferred but non-limiting embodiment of the invention, each signal frame processed by the pre-signal processing unit comes from a stored digital signal in a storage block, and the signal frame is not initially processed after the formation of the whole fluorescence digital signal, but the initial processing and transmission task of the signal frame is performed before the storage block forms the whole fluorescence digital signal.
In a preferred but non-limiting embodiment of the present invention, the storage block stores the fluorescent digital signals by using an in-sequence storage method, the in-sequence storage method stores the received digital signals according to the order of the pointers, and the processing obtains the signal frames by using a square matrix reduction method, the square matrix reduction method reduces the digital signals pointed by the pointers of one square matrix to obtain the values of the signal frames, the whole message cannot be obtained only according to one signal frame, the background device receives the actual values of the whole signal frames of the whole data, and the reliability of the signal is enhanced; the reduction method can employ an RLE algorithm for reduction.
Specifically, the signal frame includes an identifier, an azimuth symbol, a digital signal value and a terminator, the identifier is used to confirm whether a pair of signal frames is a digital signal belonging to a whole, the azimuth symbol is used to indicate the corresponding azimuth of the signal frame, the digital signal value is a signal value which is used as the actual value of the digital signal after the digital signal pointed by the corresponding square matrix pointer is reduced, the terminator has two types of normal symbols and stop symbols, when the terminator is a normal symbol, the signal frame is a normal signal frame, when the terminator is a stop symbol, the signal frame is the last signal frame of the whole fluorescence digital signal, and the stop Fu Naju has the values of the highest row number and the highest column number of the azimuth symbol;
the examples illustrate that: the pointers in a storage block are one to one hundred, are arranged by using a matrix with ten rows and ten columns, and are processed by using a matrix with two rows and two columns to obtain a signal frame, namely the matrix with ten rows and ten columns is divided into square matrix pointers with five rows and five columns according to the size of the matrix with two rows and two columns as a unit size, a digital signal pointed by one pointer in the square matrix pointer with five rows and columns represents a signal frame, the signal frame has digital signals pointed by the pointers in the corresponding matrix with two rows and two columns, the azimuths [ O ] and P ] of the signal frame are the azimuths [ O ] and P ] of the square matrix in which the pointers are located (namely the rows O and the columns P in the square matrix), the categories of O and P are positive integers from 1 to 5, the pointers are digital signal values of the reduced digital signals pointed by one, two, eleven and fifteen, which form the digital signal frame of the azimuths [ 1;1 ] 5754, the pointers are the digital signals pointed by the five, thirty-rows and thirty-six-column (thirty-column) of the pointers are the normal ending signals which form the azimuths 3552, and the highest azimuths of the ending signals are the normal ending signals 3532, which are the ending signals of the ending sign 3552.
The invention has the advantages that compared with the prior art, the invention can reach a good level of real-time function and efficiency, and the value of the signal frame is obtained by using the mode of square matrix reduction, so that the digital signal can be initially processed when some digital signals are obtained, the digital signals are synchronously matrixed and various conversions are executed for guaranteeing the real-time function, and the transmission is executed after the initial digital signal capacity is extremely reduced, thereby guaranteeing the speed and the efficiency.
In a preferred but non-limiting embodiment of the invention, an icon for illumination is clicked on an interactive interface on the touch screen, the touch screen transmits a signal of the clicked icon for illumination to the controller, and the controller sends a high-level signal to the LED illuminating lamp to enable the LED illuminating lamp to be lightened;
and clicking the icon for sterilizing on the interactive interface on the touch screen, transmitting a signal of clicking the icon for sterilizing into the controller by the touch screen, and transmitting a high-level signal to the ultraviolet lamp by the controller to realize the function of sterilizing.
Therefore, soft control is achieved by combining the interface on the touch screen to control the LED illuminating lamp and the ultraviolet lamp, so that the efficiency is improved, and the control is accurate.
The present applicant has described and illustrated embodiments of the present invention in detail with reference to the accompanying drawings, but it should be understood by those skilled in the art that the above embodiments are only preferred embodiments of the present invention, and the detailed description is only for the purpose of helping the reader to better understand the spirit of the present invention, and is not intended to limit the scope of the present invention, and on the contrary, any modifications or modifications based on the spirit of the present invention should fall within the scope of the present invention.
Claims (6)
1. A nucleic acid extraction detection control system comprising:
a receiver main body of the nucleic acid extraction detection control system and a receiver shell, wherein the receiver main body is arranged in the receiver shell;
the receiver main body comprises a plurality of paths of fluorescent signal acquisition channels, and each path of fluorescent signal acquisition channel is uniformly connected with a controller; the controller is also connected with the mobile communication module and the external memory, and the controller is connected with a background device in the mobile communication network through the mobile communication module; the fluorescence signal acquisition channel comprises a laser emitter, a fluorescence signal detection piece and a PCR tube containing nucleic acid to be detected, wherein the laser emitter, the fluorescence signal detection piece and the PCR tube are arranged in a shell of the nucleic acid extraction detection control system;
each fluorescence signal acquisition channel is used for accessing a source fluorescence signal and outputting a fluorescence digital signal to a controller;
the components running on the controller comprise a signal receiving component, a signal preprocessing component, a signal transmitting component and a receiver operating component, wherein the signal receiving component is used for receiving the fluorescent signals transmitted by the fluorescent signal acquisition channel, the signal preprocessing component is used for performing framing processing on the fluorescent digital signals to obtain signal frames, the signal transmitting component transmits the signal frames to the background device through the mobile communication module, the background device restores the signal frames to initial fluorescent digital signals and then performs analysis, the receiver operating component is used for controlling a receiving mode of the signal receiving component and a processing mode of the signal preprocessing component, and indexes confirming the receiving mode and the processing mode are transmitted to the receiver operating component through the background device and the mobile communication module;
the signal pre-processing component divides the received fluorescent digital signal into a plurality of digital signals which are individually treated as a whole according to the indexes transmitted by the background device, and then treats each digital signal frame which is individually treated as a whole into a plurality of signal frames, wherein each signal frame comprises an identifier, an azimuth symbol, a digital signal value and an abort symbol, the identifier is used for representing the identifier of the digital signal which is individually treated as a whole and is positioned in the signal frame, the azimuth symbol is used for representing the azimuth of the signal frame in the whole signal frame under the identifier, the digital signal value is the actual value of the corresponding digital signal, and the abort symbol is used for representing whether the signal frame is the last signal frame under the identifier;
the digital signal corresponding to a signal frame is the digital signal pointed by an array pointer, and is represented by a matrix H:
here, x ef Representing the value of the digital signal pointed by a corresponding pointer and being a component of the matrix, r being the rank of the pointer of the square matrix, e and f ranging from 1 to rA positive integer, r is a positive integer;
the pre-signal processing component is passed through an indicator l uv Setting the matrix H as an equal-rank matrix I:
here, d ef Is a component within matrix I;
under the condition that e is equal to 1, d ef =x ef On condition that e is higher than f, d ef A case of 0;
the index l uv The method accords with the following steps:
here, u and v are both indices l uv U is a positive integer from 1 to r-1, v is a positive integer from 1 to u +1, g is a positive integer from 2 to r, c is a positive integer from 1 to g, and g and c are used to represent the actual amounts of u and v, respectively;
the pre-signal processing component combines a component with a value other than 0 with an indicator l uv Passing to the background device as a value within a signal frame; the signal pre-processing component comprises a temporary storage queue which is divided into more than two storage blocks, one storage block is used for storing a digital signal which is used as a whole, the pointer of one storage block is provided with a same mark, the pointer of a different storage block is provided with a different mark, the signal pre-processing component acquires the stored digital signal according to the mark of the pointer and forms a signal frame,
the storage block stores the fluorescent digital signals by using an in-sequence storage method, the in-sequence storage method stores the received digital signals according to the sequence of the pointers, and the storage block processes the received digital signals to obtain the signal frames by using a square matrix reduction method, wherein the square matrix reduction method is used for reducing the digital signals pointed by the pointers of one square matrix to obtain the values of the signal frames.
2. The nucleic acid extraction detection control system of claim 1, wherein the abort symbol indicates that the signal frame is the last signal frame under an identifier, and the abort symbol of another signal frame is a normal symbol, and the abort symbol further has the value of the highest row number and the highest column number in the azimuth symbol.
3. The nucleic acid extraction detection control system according to claim 1, wherein the background device performs grouping according to identifiers of received signal frames, performs arrangement according to orientation symbols of the signal frames, runs a timer after receiving a signal frame with an abort symbol, confirms whether to receive all signal frames under the identifier within a set time period of the timer, and if there is a missing signal frame after the time period timed by the timer exceeds the set time period, transfers the corresponding identifier and orientation symbol to the pre-signal handling component via the mobile communication module to request the missing signal frame to be transferred again;
after receiving all signal frames under an identifier, the background device transmits the identifier and a confirmation message to the signal pre-processing assembly through the mobile communication module, and a temporary storage queue in the signal pre-processing assembly returns to zero according to the digital signal in a storage block of the corresponding identifier in the confirmation message, and synchronously removes marks of the corresponding pointer.
4. The nucleic acid extraction detection control system of claim 1, wherein a touch screen is embedded on an outer wall of a housing of the nucleic acid extraction detection control system, the controller is electrically connected with the touch screen, and an LED illumination lamp and an ultraviolet lamp for disinfection, both of which are connected with the controller, are further arranged in the housing of the nucleic acid extraction detection control system.
5. The method as claimed in any one of claims 1 to 4, wherein the pre-signal handling module comprises a temporary storage queue for receiving and storing the digital signals received by the signal receiving unit, the temporary storage queue performs a cutting operation on the temporary storage queue according to the specification transmitted by the background device, the cutting operation comprises the following steps:
step 1: marking the appointed pointer with a mark according to the standard index, wherein the pointer with the mark forms a storage block, and the capacity of the storage block is confirmed by the standard index;
step 2: identifying the fluorescent digital signal to be stored according to standard indexes, storing the digital signal in the storage block if the fluorescent digital signal to be stored and the fluorescent digital signal stored in the storage block belong to an integral digital signal, marking another storage block in the pointer without the mark if the digital signal to be stored and the digital signal stored in the storage block belong to a different integral digital signal, and storing the digital signal in a new storage block, wherein the different storage block has different marks;
and step 3: if the digital signal stored in the storage block is full, adding the pointer which is not marked with the mark into the storage block, and if the storage block forms an integral digital signal, removing the mark from the pointer which is not used in the storage block;
each signal frame obtained by the treatment of the signal pre-treatment component comes from a storage digital signal in a storage block, and the initial treatment and transmission task of the signal frame is executed before the storage block forms an integral fluorescent digital signal;
the storage block stores the fluorescent digital signals by using an in-sequence storage method, the in-sequence storage method stores the received digital signals according to the sequence of the pointers, and the storage block processes the received digital signals to obtain the signal frames by using a square matrix reduction method, wherein the square matrix reduction method is used for reducing the digital signals pointed by the pointers of one square matrix to obtain the values of the signal frames.
6. The control method of the control system for nucleic acid isolation detection according to claim 5, wherein an icon for illumination is clicked on the interactive interface on the touch panel, the touch panel transmits a signal indicating the click of the icon for illumination to the controller, and the controller sends a high-level signal to the LED illumination lamp to illuminate the LED illumination lamp; the icon for killing virus is clicked on the interactive interface on the touch screen, the touch screen transmits a signal of the clicked icon for killing virus to the controller, and the controller sends a high-level signal to the ultraviolet lamp to achieve the function of killing virus.
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| KR101224976B1 (en) * | 2009-01-22 | 2013-01-22 | 미쯔이 죠센 가부시키가이샤 | Fluorescence detecting device and fluorescence detecting method |
| CN105861299B (en) * | 2016-05-05 | 2018-05-22 | 广东顺德工业设计研究院(广东顺德创新设计研究院) | Droplet type digital pcr fluorescence detecting system and fluorescence detection device |
| CN107229114A (en) * | 2017-04-12 | 2017-10-03 | 上海戴泽光电科技有限公司 | Multichannel fluorescence detection system based on fiber array |
| CN107592560A (en) * | 2017-09-29 | 2018-01-16 | 北京盛世辉科技有限公司 | Control method, device, intelligent remote controller and computer-readable recording medium |
| CN111424032B (en) * | 2020-03-25 | 2022-08-30 | 广州高盛生物科技股份有限公司 | Nucleic acid extraction method, device, system, control equipment and storage medium |
| CN112957011B (en) * | 2021-02-01 | 2022-06-24 | 西安电子科技大学 | High-sensitivity weak fluorescence signal detection system, method, storage medium and application |
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