CN115216361A - Preparation method of ganoderma lucidum spore oil - Google Patents
Preparation method of ganoderma lucidum spore oil Download PDFInfo
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- CN115216361A CN115216361A CN202210968105.0A CN202210968105A CN115216361A CN 115216361 A CN115216361 A CN 115216361A CN 202210968105 A CN202210968105 A CN 202210968105A CN 115216361 A CN115216361 A CN 115216361A
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/04—Pretreatment of vegetable raw material
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- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/10—Production of fats or fatty oils from raw materials by extracting
- C11B1/104—Production of fats or fatty oils from raw materials by extracting using super critical gases or vapours
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P20/00—Technologies relating to chemical industry
- Y02P20/50—Improvements relating to the production of bulk chemicals
- Y02P20/54—Improvements relating to the production of bulk chemicals using solvents, e.g. supercritical solvents or ionic liquids
Abstract
The invention discloses a preparation method of ganoderma lucidum spore oil, which comprises the following steps: s1, pretreating ganoderma lucidum spores, removing excessive outer ganoderma lucidum spore shell wall, and only remaining inner ganoderma lucidum spore shell wall to wrap contents; s2, alternately putting the pretreated ganoderma spores into a refrigerator and a defreezer, and performing multiple freezing and unfreezing circulating embrittlement treatments; s3, uniformly mixing the ganoderma lucidum spores subjected to the circulating embrittlement treatment, vitamin E and potassium sorbate, and putting the mixture into a superfine powder wall breaking machine for low-temperature wall breaking treatment to obtain wall-broken ganoderma lucidum spore powder; s4, uniformly stirring the wall-broken ganoderma lucidum spore powder and ethanol, granulating by using a granulator to obtain ganoderma lucidum spore particles with the particle size of 0.1-0.4 cm, and drying the ganoderma lucidum spore particles; and S5, adding the dried ganoderma lucidum spore particles into a supercritical extraction kettle for extraction to obtain yellow ganoderma lucidum spore oil.
Description
Technical Field
The invention relates to the technical field of ganoderma lucidum processing, and particularly relates to a preparation method of ganoderma lucidum spore oil.
Background
The glossy ganoderma spore oil is yellow transparent liquid, and has main components of ganoderic acid, unsaturated fatty acid, glossy ganoderma polysaccharide, etc. it can induce or promote the phagocytosis of macrophage to kill tumor cell directly or indirectly, raise immunity of organism, and reduce the occurrence of poor spirit, anorexia, vomiting, alopecia, etc. caused by radiotherapy and chemotherapy. In addition, the Ganoderma spore oil has analgesic, antiinflammatory, toxic materials clearing away, liver protecting, digestive function promoting, blood cholesterol and triglyceride reducing, platelet coagulation inhibiting, thrombosis eliminating, blood viscosity reducing, hemoglobin providing, oxygen supply to heart and brain promoting, blood circulation promoting, blood vessel softening, blood purifying, free radicals eliminating, blood pressure regulating, metabolism promoting, and immunity regulating effects.
In the existing preparation process of the ganoderma lucidum spore oil, ganoderma lucidum spore powder is usually directly put into a superfine crusher for wall breaking, and then the ganoderma lucidum spore oil is separated from the ganoderma lucidum spore powder after wall breaking. Through the wall breaking treatment of the superfine powder crusher, the ganoderma lucidum spore shell wall is crushed into powder from a shell shape, the surface area of the ganoderma lucidum spore shell wall is greatly increased, the separation and purification difficulty of the ganoderma lucidum spore oil is increased, a large amount of ganoderma lucidum spore oil is easy to adhere to and even permeate into the powder ganoderma lucidum spore shell wall, and the yield of the ganoderma lucidum spore oil is reduced.
Disclosure of Invention
The invention aims to provide a preparation method of ganoderma lucidum spore oil, which skillfully combines chemical wall cutting and mechanical wall breaking together, so that the shell wall quantity of ganoderma lucidum spores during later-stage wall breaking is reduced, the shell wall is convenient to break, the wall breaking rate is high, the separation and purification difficulty of ganoderma lucidum spore oil is reduced, the adhesion and absorption of powdery ganoderma lucidum spore shell wall on ganoderma lucidum contents are greatly reduced, the yield of the ganoderma lucidum spore oil can be effectively improved, and the use effect is good.
In order to achieve the purpose, the invention adopts the following technical scheme:
a method for preparing Ganoderma spore oil comprises the following steps:
s1, pretreating ganoderma lucidum spores, removing excessive outer ganoderma lucidum spore shell wall, and only remaining inner ganoderma lucidum spore shell wall to wrap contents;
s2, alternately putting the pretreated ganoderma spores into a refrigerator and a defreezer, and performing multiple freezing and unfreezing circulating embrittlement treatments;
s3, uniformly mixing the ganoderma lucidum spores subjected to the circulating embrittlement treatment, vitamin E and potassium sorbate, and putting the mixture into a superfine powder wall breaking machine for low-temperature wall breaking treatment to obtain wall-broken ganoderma lucidum spore powder;
s4, uniformly stirring the wall-broken ganoderma lucidum spore powder and ethanol, granulating by using a granulator to obtain ganoderma lucidum spore particles with the particle size of 0.1-0.4 cm, and drying the ganoderma lucidum spore particles;
and S5, adding the dried ganoderma lucidum spore particles into a supercritical extraction kettle for extraction to obtain yellow ganoderma lucidum spore oil.
Preferably, the preprocessing procedure in step S1 is:
s11, preparing a mixed enzyme solution of cellulase, hemicellulase and pectinase, wherein the mass concentrations of the cellulase, the hemicellulase and the pectinase are respectively 2.2%, 1.6% and 1.9%;
s12, putting the ganoderma lucidum spores into a mixed enzyme solution at 25-32 ℃, and stirring until only 5% -10% of inner-layer ganoderma lucidum spore shell wall is left to wrap the content, wherein the stirring speed is 2500-3500r/min;
and S13, washing the ganoderma spores for multiple times by adopting pure water, filtering, and then freeze-drying the ganoderma spores.
Preferably, the conditions of the cyclic embrittlement treatment in step S2 are: the freezing temperature is-15 to-10 ℃, the freezing time is 10 to 15min, the unfreezing time is 3 to 10min, and the cycle times of freezing and unfreezing are 2 to 3 times.
Preferably, the adding proportion of the ganoderma lucidum spores, the vitamin E and the potassium sorbate in the step S3 is (85-90): (2-5): (1-3).
Preferably, the temperature of the low-temperature wall breaking treatment in the step S3 is-8 to-5 ℃, and the temperature is in CO 2 Under the protection of the atmosphere of (2), wall breaking is carried out.
Preferably, the mass of the ethanol added in the step S4 is 4-8% of the ganoderma lucidum spore powder.
Preferably, the specific process of step S5 is: drying Ganoderma spore granulePlacing in supercritical CO 2 In the extraction kettle, supercritical CO is carried out 2 Extracting, wherein the extraction pressure of an extraction kettle is 24-30MPa, and the extraction temperature of the extraction kettle is 35-40 ℃; performing two-stage separation by a separation kettle, wherein the separation pressure of a first-stage separation kettle is 14-18MPa, the separation temperature of the first-stage separation kettle is 40-50 ℃, the separation pressure of a second-stage separation kettle is 3-6MPa, and the separation temperature of the second-stage separation kettle is 35-40 ℃; and (4) obtaining yellow ganoderma lucidum spore oil after complete extraction.
Compared with the background art, the invention has the following beneficial effects:
1. the method skillfully combines chemical wall cutting and mechanical wall breaking together, pretreats the ganoderma lucidum spores, removes excessive ganoderma lucidum spore shell walls on the outer layers, only leaves a thin ganoderma lucidum spore shell wall on the inner layer to wrap the content, effectively reduces the wall thickness of the ganoderma lucidum spores, reduces the shell wall amount of the ganoderma lucidum spores during later-stage wall breaking, is convenient to break the shell wall, has high wall breaking rate, reduces the separation and purification difficulty of ganoderma lucidum spore oil, greatly reduces the adhesion and absorption of the ganoderma lucidum content by the powdery ganoderma lucidum spore shell wall, can effectively improve the yield of the ganoderma lucidum spore oil, and has good use effect.
2. The method performs circulating embrittlement treatment on the ganoderma lucidum spores with the shell walls thinned to 5% -10% by freezing and unfreezing, can quickly catalyze the thinned ganoderma lucidum spore shell walls, and is convenient for mechanically breaking the ganoderma lucidum spores in the later period.
3. The invention takes vitamin E as an antioxidant, potassium sorbate as a preservative and CO as 2 The ganoderma lucidum spore is subjected to low-temperature wall breaking treatment at the low temperature of-8 to-5 ℃ for protecting atmosphere, so that the deterioration phenomena of nutrient loss, rancidity and the like caused by wall breaking of the ganoderma lucidum spore can be effectively reduced, and the quality of the ganoderma lucidum spore oil can be effectively improved.
4. The invention evenly stirs the wall-broken ganoderma lucidum spore powder and ethanol, obtains 0.1-0.4 cm ganoderma lucidum spore particles through granulation and drying treatment, and then places the ganoderma lucidum spore particles in supercritical CO 2 The second-stage separation and extraction are carried out in the extraction kettle, so that the yield of the ganoderma lucidum spore oil can be effectively improved.
Drawings
FIG. 1 is a block flow diagram of the present invention.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and do not limit the invention.
A method for preparing Ganoderma spore oil is described with reference to figure 1.
Example 1
A method for preparing Ganoderma spore oil comprises the following steps:
s1, pretreating ganoderma lucidum spores, removing excessive outer ganoderma lucidum spore shell wall, and only remaining inner ganoderma lucidum spore shell wall to wrap contents;
the pretreatment process in the step S1 is as follows:
s11, preparing a mixed enzyme solution of cellulase, hemicellulase and pectinase, wherein the mass concentrations of the cellulase, the hemicellulase and the pectinase are respectively 2.2%, 1.6% and 1.9%; s12, putting the ganoderma lucidum spores into a mixed enzyme solution at 28 ℃, and stirring until only an inner ganoderma lucidum spore shell wall with the wall thickness of about 8% is left to wrap the content, wherein the stirring speed is 3200r/min; s13, washing the ganoderma spores by pure water for 3 times, filtering, and then freeze-drying the ganoderma spores;
s2, alternately putting the pretreated ganoderma lucidum spores into a refrigerator and a thawing machine, and performing freezing and thawing circulating embrittlement treatment for multiple times;
the conditions of the cyclic embrittlement treatment in step S2 are: the freezing temperature is-12 ℃, the freezing time is 12min, the thawing time is 6min, and the cycle times of freezing and thawing are 3 times;
s3, uniformly mixing the ganoderma lucidum spores subjected to the circulating embrittlement treatment, vitamin E and potassium sorbate, and putting the mixture into a superfine powder wall breaking machine for low-temperature wall breaking treatment to obtain ganoderma lucidum spore powder subjected to wall breaking, wherein the ganoderma lucidum spores, the vitamin E and the potassium sorbate are addedAdding the following components in a ratio of 88 2 Breaking the cell wall under the protection of the atmosphere;
s4, uniformly stirring the ganoderma lucidum spore powder subjected to wall breaking and ethanol, granulating by using a granulator to obtain ganoderma lucidum spore particles with the particle size of 0.3cm, and drying the ganoderma lucidum spore particles, wherein the mass of the ethanol is 5% of that of the ganoderma lucidum spore powder;
s5, placing the dried ganoderma lucidum spore particles in supercritical CO 2 In an extraction kettle, supercritical CO is carried out 2 Extracting, wherein the extraction pressure of an extraction kettle is 27MPa, and the extraction temperature of the extraction kettle is 38 ℃; performing two-stage separation by a separation kettle, wherein the separation pressure of a first-stage separation kettle is 16MPa, the separation temperature of the first-stage separation kettle is 45 ℃, the separation pressure of a second-stage separation kettle is 4MPa, and the separation temperature of the second-stage separation kettle is 37 ℃; and (4) obtaining yellow ganoderma lucidum spore oil after complete extraction.
Example 2
A method for preparing Ganoderma spore oil comprises the following steps:
s1, pretreating ganoderma lucidum spores, removing excessive outer ganoderma lucidum spore shell wall, and only remaining inner ganoderma lucidum spore shell wall to wrap contents;
the pretreatment process in the step S1 is as follows:
s11, preparing a mixed enzyme solution of cellulase, hemicellulase and pectinase, wherein the mass concentrations of the cellulase, the hemicellulase and the pectinase are respectively 2.2%, 1.6% and 1.9%; s12, putting the ganoderma lucidum spores into a mixed enzyme solution at 30 ℃, and stirring until only an inner ganoderma lucidum spore shell wall with the wall thickness of about 8% is left to wrap the contents, wherein the stirring speed is 3500r/min; s13, washing the ganoderma lucidum spores for 3 times by adopting pure water, filtering, and then freeze-drying the ganoderma lucidum spores;
s2, alternately putting the pretreated ganoderma lucidum spores into a refrigerator and a thawing machine, and performing freezing and thawing circulating embrittlement treatment for multiple times;
the conditions of the cyclic embrittlement treatment in step S2 are: the freezing temperature is-15 ℃, the freezing time is 15min, the thawing time is 8min, and the cycle times of freezing and thawing are 3 times;
s3, uniformly mixing the ganoderma lucidum spores subjected to the circulating embrittlement treatment, vitamin E and potassium sorbate, and putting the mixture into a superfine powder wall breaking machine for low-temperature wall breaking treatment to obtain ganoderma lucidum spore powder subjected to wall breaking, wherein the adding ratio of the ganoderma lucidum spores, the vitamin E and the potassium sorbate is 85 2 Breaking the cell wall under the protection of the atmosphere;
s4, uniformly stirring the ganoderma lucidum spore powder subjected to wall breaking and ethanol, granulating by using a granulator to obtain ganoderma lucidum spore particles with the particle size of 0.3cm, and drying the ganoderma lucidum spore particles, wherein the mass of the ethanol is 4% of that of the ganoderma lucidum spore powder;
s5, placing the dried ganoderma lucidum spore particles in supercritical CO 2 In an extraction kettle, supercritical CO is carried out 2 Extracting, wherein the extraction pressure of an extraction kettle is 25MPa, and the extraction temperature of the extraction kettle is 36 ℃; performing two-stage separation by a separation kettle, wherein the separation pressure of a first-stage separation kettle is 17MPa, the separation temperature of the first-stage separation kettle is 43 ℃, the separation pressure of a second-stage separation kettle is 5MPa, and the separation temperature of the second-stage separation kettle is 36 ℃; and (4) obtaining yellow ganoderma lucidum spore oil after complete extraction.
Example 3
A method for preparing Ganoderma spore oil comprises the following steps:
s1, pretreating ganoderma lucidum spores, removing excessive ganoderma lucidum spore shell walls on the outer layer, and only remaining inner ganoderma lucidum spore shell walls to wrap contents;
the pretreatment process in the step S1 comprises the following steps:
s11, preparing a mixed enzyme solution of cellulase, hemicellulase and pectinase, wherein the mass concentrations of the cellulase, the hemicellulase and the pectinase are respectively 2.2%, 1.6% and 1.9%; s12, putting the ganoderma lucidum spores into a mixed enzyme solution at 28 ℃, and stirring until only an inner ganoderma lucidum spore shell wall with the wall thickness of about 8% is left to wrap the contents, wherein the stirring speed is 3200r/min; s13, washing the ganoderma spores by pure water for 3 times, filtering, and then freeze-drying the ganoderma spores;
s2, alternately putting the pretreated ganoderma lucidum spores into a refrigerator and a thawing machine, and performing freezing and thawing circulating embrittlement treatment for multiple times;
the conditions of the cyclic embrittlement treatment in step S2 are: the freezing temperature is-12 ℃, the freezing time is 13min, the thawing time is 5min, and the cycle times of freezing and thawing are 3 times;
s3, uniformly mixing the ganoderma lucidum spores subjected to the circulating embrittlement treatment, vitamin E and potassium sorbate, and putting the mixture into a superfine powder wall breaking machine for low-temperature wall breaking treatment to obtain ganoderma lucidum spore powder subjected to wall breaking, wherein the adding ratio of the ganoderma lucidum spores, the vitamin E and the potassium sorbate is 89 2 Under the protection of the atmosphere, wall breaking is carried out;
s4, uniformly stirring the wall-broken ganoderma lucidum spore powder and ethanol, granulating by using a granulator to obtain ganoderma lucidum spore particles with the particle size of 0.3cm, and drying the ganoderma lucidum spore particles, wherein the mass of the ethanol added is 7% of that of the ganoderma lucidum spore powder;
s5, placing the dried ganoderma lucidum spore particles in supercritical CO 2 In an extraction kettle, supercritical CO is carried out 2 Extracting, wherein the extraction pressure of an extraction kettle is 28MPa, and the extraction temperature of the extraction kettle is 38 ℃; performing two-stage separation by a separation kettle, wherein the separation pressure of the first-stage separation kettle is 14MPa, the separation temperature of the first-stage separation kettle is 42 ℃, the separation pressure of the second-stage separation kettle is 5MPa, and the separation temperature of the second-stage separation kettle is 35 ℃; and (4) obtaining yellow ganoderma lucidum spore oil after complete extraction.
Example 4
A method for preparing Ganoderma spore oil comprises the following steps:
s1, pretreating ganoderma lucidum spores, removing excessive outer ganoderma lucidum spore shell wall, and only remaining inner ganoderma lucidum spore shell wall to wrap contents;
the pretreatment process in the step S1 is as follows:
s11, preparing a mixed enzyme solution of cellulase, hemicellulase and pectinase, wherein the mass concentrations of the cellulase, the hemicellulase and the pectinase are respectively 2.2%, 1.6% and 1.9%; s12, putting the ganoderma lucidum spores into a mixed enzyme solution at 32 ℃, and stirring until only an inner layer ganoderma lucidum spore shell wall with the wall thickness of about 7% is left to wrap the contents, wherein the stirring speed is 3500r/min; s13, washing the ganoderma spores by pure water for 3 times, filtering, and then freeze-drying the ganoderma spores;
s2, alternately putting the pretreated ganoderma lucidum spores into a refrigerator and a thawing machine, and performing freezing and thawing circulating embrittlement treatment for multiple times;
the conditions of the cyclic embrittlement treatment in step S2 are: the freezing temperature is-10 ℃, the freezing time is 15min, the thawing time is 10min, and the cycle times of freezing and thawing are 3 times;
s3, uniformly mixing the ganoderma lucidum spores subjected to the circulating embrittlement treatment, vitamin E and potassium sorbate, and putting the mixture into a superfine powder wall breaking machine for low-temperature wall breaking treatment to obtain ganoderma lucidum spore powder subjected to wall breaking, wherein the adding ratio of the ganoderma lucidum spores, the vitamin E and the potassium sorbate is 90 2 Under the protection of the atmosphere, wall breaking is carried out;
s4, uniformly stirring the wall-broken ganoderma lucidum spore powder and ethanol, granulating by using a granulator to obtain ganoderma lucidum spore particles with the particle size of 0.3cm, and drying the ganoderma lucidum spore particles, wherein the mass of the ethanol added is 8% of that of the ganoderma lucidum spore powder;
s5, placing the dried ganoderma lucidum spore particles in supercritical CO 2 In the extraction kettle, supercritical CO is carried out 2 Extracting, wherein the extraction pressure of an extraction kettle is 30MPa, and the extraction temperature of the extraction kettle is 37 ℃; performing two-stage separation by a separation kettle, wherein the separation pressure of a first-stage separation kettle is 18MPa, the separation temperature of the first-stage separation kettle is 50 ℃, the separation pressure of a second-stage separation kettle is 6MPa, and the separation temperature of the second-stage separation kettle is 40 ℃; and obtaining yellow ganoderma lucidum spore oil after complete extraction.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any changes or substitutions that can be easily conceived by those skilled in the art within the technical scope of the present invention are included in the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the claims.
Claims (7)
1. The preparation method of the ganoderma lucidum spore oil is characterized by comprising the following steps:
s1, pretreating ganoderma lucidum spores, removing excessive ganoderma lucidum spore shell walls on the outer layer, and only remaining inner ganoderma lucidum spore shell walls to wrap contents;
s2, alternately putting the pretreated ganoderma lucidum spores into a refrigerator and a thawing machine, and performing freezing and thawing circulating embrittlement treatment for multiple times;
s3, uniformly mixing the ganoderma lucidum spores subjected to the circulating embrittlement treatment, vitamin E and potassium sorbate, and putting the mixture into a superfine powder wall breaking machine for low-temperature wall breaking treatment to obtain wall-broken ganoderma lucidum spore powder;
s4, uniformly stirring the wall-broken ganoderma lucidum spore powder and ethanol, granulating by using a granulator to obtain ganoderma lucidum spore particles with the particle size of 0.1-0.4 cm, and drying the ganoderma lucidum spore particles;
and S5, adding the dried ganoderma lucidum spore particles into a supercritical extraction kettle for extraction to obtain yellow ganoderma lucidum spore oil.
2. The method for preparing ganoderma lucidum spore oil as claimed in claim 1, wherein the pretreatment process in the step S1 is:
s11, preparing a mixed enzyme solution of cellulase, hemicellulase and pectinase, wherein the mass concentrations of the cellulase, the hemicellulase and the pectinase are respectively 2.2%, 1.6% and 1.9%;
s12, putting the ganoderma lucidum spores into a mixed enzyme solution at 25-32 ℃, and stirring until only 5% -10% of inner-layer ganoderma lucidum spore shell wall is left to wrap the content, wherein the stirring speed is 2500-3500r/min;
and S13, washing the ganoderma lucidum spores for multiple times by adopting pure water, filtering, and then freeze-drying the ganoderma lucidum spores.
3. The method for preparing ganoderma lucidum spore oil according to claim 1, wherein the conditions of the circulating embrittlement treatment in the step S2 are as follows: the freezing temperature is-15 to-10 ℃, the freezing time is 10 to 15min, the unfreezing time is 3 to 10min, and the cycle times of freezing and unfreezing are 2 to 3 times.
4. The method for preparing ganoderma lucidum spore oil as claimed in claim 1, wherein the method comprises the following steps: in the step S3, the adding proportion of the ganoderma lucidum spores, the vitamin E and the potassium sorbate is (85-90): (2-5): 1-3).
5. The method for preparing ganoderma lucidum spore oil according to claim 1, wherein the temperature of the low-temperature wall breaking treatment in step S3 is-8 to-5 ℃, and the temperature is CO 2 Under the protection of the atmosphere.
6. The method for preparing ganoderma lucidum spore oil as claimed in claim 1, wherein the method comprises the following steps: in the step S4, the mass of the added ethanol is 4-8% of the ganoderma lucidum spore powder.
7. The method for preparing ganoderma lucidum spore oil as claimed in claim 1, wherein the specific process of the step S5 is as follows: placing the dried Ganoderma spore particles in supercritical CO 2 In the extraction kettle, supercritical CO is carried out 2 Extracting, wherein the extraction pressure of an extraction kettle is 24-30MPa, and the extraction temperature of the extraction kettle is 35-40 ℃; performing two-stage separation by a separation kettle, wherein the separation pressure of a first-stage separation kettle is 14-18MPa, the separation temperature of the first-stage separation kettle is 40-50 ℃, the separation pressure of a second-stage separation kettle is 3-6MPa, and the separation temperature of the second-stage separation kettle is 35-40 ℃; and (4) obtaining yellow ganoderma lucidum spore oil after complete extraction.
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| CN103981018A (en) * | 2014-04-23 | 2014-08-13 | 珀莱雅化妆品股份有限公司 | Preparation method of ganoderma lucidum spores oil |
| CN106318600A (en) * | 2016-09-11 | 2017-01-11 | 开平市民丰食品有限公司 | Method for preparing ganoderma spore oil |
| CN106318612A (en) * | 2016-09-19 | 2017-01-11 | 中山大学 | Method for supercritical extraction of ganoderma lucidum spore oil |
| CN108485794A (en) * | 2018-02-06 | 2018-09-04 | 青岛润达生物科技有限公司 | A kind of preparation method of ganoderma lucidum spore oil |
| CN112680275A (en) * | 2021-03-22 | 2021-04-20 | 南昌之禾生物工程有限公司 | Supercritical extraction process of ganoderma lucidum spore oil with high extraction rate |
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