CN115212235A - Production method of rabbit fur capable of causing inflammation by diluted vaccinia virus - Google Patents
Production method of rabbit fur capable of causing inflammation by diluted vaccinia virus Download PDFInfo
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- CN115212235A CN115212235A CN202210878980.XA CN202210878980A CN115212235A CN 115212235 A CN115212235 A CN 115212235A CN 202210878980 A CN202210878980 A CN 202210878980A CN 115212235 A CN115212235 A CN 115212235A
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- vaccinia virus
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- 241000283973 Oryctolagus cuniculus Species 0.000 title claims abstract description 62
- 241000700618 Vaccinia virus Species 0.000 title claims abstract description 57
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 27
- 206010061218 Inflammation Diseases 0.000 title claims abstract description 13
- 230000004054 inflammatory process Effects 0.000 title description 4
- 238000007710 freezing Methods 0.000 claims abstract description 13
- 230000008014 freezing Effects 0.000 claims abstract description 13
- 238000004806 packaging method and process Methods 0.000 claims abstract description 4
- 238000010008 shearing Methods 0.000 claims abstract description 4
- 210000001550 testis Anatomy 0.000 claims description 18
- 238000010790 dilution Methods 0.000 claims description 5
- 239000012895 dilution Substances 0.000 claims description 5
- 238000002347 injection Methods 0.000 claims description 4
- 239000007924 injection Substances 0.000 claims description 4
- 238000007865 diluting Methods 0.000 claims description 3
- 238000005360 mashing Methods 0.000 claims description 3
- 238000010257 thawing Methods 0.000 claims description 3
- 206010046865 Vaccinia virus infection Diseases 0.000 claims 2
- 208000007089 vaccinia Diseases 0.000 claims 2
- 239000002994 raw material Substances 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 3
- 239000007787 solid Substances 0.000 abstract description 3
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 230000002757 inflammatory effect Effects 0.000 description 20
- 239000000427 antigen Substances 0.000 description 2
- 102000036639 antigens Human genes 0.000 description 2
- 108091007433 antigens Proteins 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 238000005406 washing Methods 0.000 description 2
- 238000001816 cooling Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- A61K35/36—Skin; Hair; Nails; Sebaceous glands; Cerumen; Epidermis; Epithelial cells; Keratinocytes; Langerhans cells; Ectodermal cells
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
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- Animal Behavior & Ethology (AREA)
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- Environmental Sciences (AREA)
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- Pharmacology & Pharmacy (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Dermatology (AREA)
- Epidemiology (AREA)
- Developmental Biology & Embryology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Animal Husbandry (AREA)
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- Engineering & Computer Science (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a production method of diluted vaccinia virus inflammation-causing rabbit fur, which comprises the following steps: firstly, selecting an inflammation-causing rabbit meeting the requirement of producing an extract; secondly, feeding and observing for more than two days and shearing; thirdly, feeding and observing the diluted vaccinia virus; fourthly, rapidly freezing and collecting rabbit skins; and step five, packaging, warehousing and freezing treatment, and the invention has the beneficial effects that: the production efficiency is improved; the method lays a solid foundation for the future enlargement of production and supply of sufficient raw materials.
Description
Technical Field
The invention relates to the technical field of inflammatory rabbit fur production, and particularly relates to a production method of diluted vaccinia virus inflammatory rabbit fur.
Background
With the increasing market share of the rabbit fur extract injection caused by vaccinia virus, the yield of the rabbit fur extract injection is increased; therefore, the yield of the raw material vaccinia virus-induced rabbit skin is required to be greatly increased, the vaccinia virus seeds are an important raw material of the vaccinia virus-induced rabbit skin, the batch yield is low at present, the cost is high, the requirement on the production process is high, the high-risk vaccinia virus seeds belong to high-risk products, and the bottleneck for restricting the production capacity of the inflammation-induced rabbit skin is formed.
The application of vaccinia virus seeds still has a large promotion space, and in order to meet the requirements of increasing the yield of products and reducing the cost, the research on the optimal dilution concentration of vaccinia virus seeds in application and the improvement of the utilization efficiency of vaccinia virus seeds are the problems which need to be solved urgently at present.
An effective solution to the problems in the related art has not been proposed yet.
Disclosure of Invention
Technical problem to be solved
Aiming at the defects of the prior art, the invention provides a production method of diluted vaccinia virus inflammation-causing rabbit fur, and aims to solve the problems in the background technology.
(II) technical scheme
In order to realize the advantages, the specific technical scheme adopted by the invention comprises the following operation steps:
firstly, selecting an inflammation-causing rabbit meeting the production requirement of an extract; secondly, feeding and observing for more than two days and shearing; thirdly, feeding and observing the diluted vaccinia virus; fourthly, rapidly freezing and collecting rabbit skins; and fifthly, packaging, warehousing and freezing.
Further, the preparation method of the diluted vaccinia virus comprises the following steps:
selecting male rabbits for passage; step two, quarantine observation; step three, inoculating the male rabbits; step four, feeding and observing the inoculated male rabbits; step five, taking testicles of male rabbits; step six, repairing and washing the testicles; seventhly, mashing the adopted testis; step eight, freezing and thawing the mashed testis; step nine, centrifuging the frozen and thawed testicles; step ten, subpackaging the centrifuged testis; and step eleven, diluting the vaccinia virus.
Further, the dilution factor of the vaccinia virus is 1000-fold.
Further, the vaccinia virus after injection dilution is fed to observe the pox occurrence: the number of the pox with the diameter of 1-2 cm is more than 80, and the number of the pox with the diameter of less than 1cm is more than 100.
Furthermore, the rapid freezing adopts the rapid cooling temperature of the rabbit skin of-18 ℃, the adopted rabbit skin needs to be complete, the color is reddish, no putrefactive odor is generated, and the pox is purplish red.
Further, the fifth step, the sixth step, the seventh step, the tenth step and the eleventh step are all performed inside the biosafety cabinet.
(III) advantageous effects
Compared with the prior art, the invention provides a production method of diluted vaccinia virus inflammation-causing rabbit skin, which has the following beneficial effects:
1. after the vaccinia virus seeds are diluted by 1000 times, the inflammatory rabbit skin meeting the quality standard of the inflammatory rabbit skin caused by vaccinia virus can be produced, and the production requirement for the inflammatory rabbit skin can be met only by producing 5 batches of vaccinia virus seeds; 6 batches of vaccinia virus seeds are produced in a small amount, and the operating cost of workshop facilities can be saved by 40 ten thousand yuan; a large amount of labor and plant facility operating cost is saved for a company;
2. if the vaccinia virus seeds can produce the inflammatory rabbit skin meeting the quality standard of the inflammatory rabbit skin of the vaccinia virus seeds after being diluted by 1000 times, 22500 sheets of inflammatory rabbit skin can be produced by each batch of antigen, 67.5 ten thousand sheets of inflammatory rabbit skin can be produced every year, and a solid foundation is laid for the future production expansion and sufficient raw material supply.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings needed in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.
FIG. 1 is a schematic flow chart of the inflammatory rabbit skin method of the present invention;
FIG. 2 is a flow chart of the preparation of diluted vaccinia virus according to the invention.
Detailed Description
For further explanation of the various embodiments, the drawings which form a part of the disclosure and which are incorporated in and constitute a part of this specification, illustrate embodiments and, together with the description, serve to explain the principles of operation of the embodiments, and to enable others of ordinary skill in the art to understand the various embodiments and advantages of the invention, and, by reference to these figures, reference is made to the accompanying drawings, which are not to scale and wherein like reference numerals generally refer to like elements.
According to the embodiment of the invention, a production method of diluted vaccinia virus inflammatory rabbit skin is provided.
The present invention will be further described with reference to the accompanying drawings and the detailed description thereof, as shown in fig. 1-2, according to the method for producing diluted vaccinia virus inflammatory rabbit skin of the embodiment of the present invention, in the first step, rabbits meeting the requirement of producing extract are selected for inflammation; step two, raising and observing for more than two days and shearing; thirdly, feeding and observing the diluted vaccinia virus; fourthly, rapidly freezing and collecting rabbit skins; and fifthly, packaging, warehousing and freezing.
In one embodiment, the method for preparing a diluted vaccinia virus comprises the steps of:
selecting male rabbits for passage; step two, quarantine observation; step three, inoculating the male rabbits; step four, feeding and observing the inoculated male rabbits; step five, taking testicles of male rabbits; step six, repairing and washing the testicles; seventhly, mashing the adopted testis; step eight, freezing and thawing the mashed testis; step nine, centrifuging the frozen and thawed testicles; step ten, subpackaging the centrifuged testis; and step eleven, diluting the vaccinia virus.
In one embodiment, the vaccinia virus is diluted 1000-fold.
In one embodiment, the diluted vaccinia virus feed is administered to observe pox onset: the number of the pox with the diameter of 1-2 cm is more than 80, and the total number of the pox with the diameter of less than 1cm is more than 100.
In one embodiment, the rapid freezing temperature of the rabbit skin is-18 ℃, the rabbit skin needs to be complete, reddish in color, without putrefactive odor, and purplish red in whelk.
In one embodiment, the fifth step, the sixth step, the seventh step, the tenth step and the eleventh step are all performed inside the biosafety cabinet.
Although the production cost of batches of vaccinia virus seeds is not changed greatly (about 200 yuan/ml), the use cost of each milliliter of vaccinia virus seeds is obviously reduced, if 10 ten thousand of inflammatory rabbit skins are produced in one year, and the current vaccinia virus seeds are diluted by 1; if the vaccinia virus seeds can produce the inflammatory rabbit skin meeting the quality standard of the inflammatory rabbit skin of the vaccinia virus after being diluted by 1000 times, the production requirement for the inflammatory rabbit skin can be met only by producing 5 batches of vaccinia virus seeds; 6 batches of vaccinia virus seeds are produced in a small amount, and the operating cost of workshop facilities can be saved by 40 ten thousand yuan; in addition, only 2 months are needed for producing 5 batches of vaccinia virus seeds, and the workers in the vaccinia virus seed workshop can be distributed to other workshops for 10 months, so that a large amount of labor and plant facility operating cost are saved for a company.
If 30 batches of vaccinia virus seeds are produced at full load in one year, the yield of one batch of vaccinia virus seeds is about 600ml at present, the dilution multiple of the current vaccinia virus seeds is 1; if the vaccinia virus seeds can produce the inflammatory rabbit skin meeting the quality standard of the inflammatory rabbit skin of the vaccinia virus seeds after being diluted by 1000 times, 22500 sheets of inflammatory rabbit skin can be produced by each batch of antigen, 67.5 ten thousand sheets of inflammatory rabbit skin can be produced every year, and a solid foundation is laid for the future production expansion and sufficient raw material supply.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and should not be taken as limiting the scope of the present invention, which is intended to cover any modifications, equivalents, improvements, etc. within the spirit and scope of the present invention.
Claims (6)
1. A production method of diluted vaccinia virus inflammation-causing rabbit fur is characterized by comprising the following steps: firstly, selecting an inflammation-causing rabbit meeting the production requirement of an extract; secondly, feeding and observing for more than two days and shearing; thirdly, feeding and observing the diluted vaccinia virus; fourthly, rapidly freezing and collecting rabbit skins; and fifthly, packaging, warehousing and freezing.
2. The method for producing diluted vaccinia virus inflammation-causing rabbit fur according to claim 1, wherein the method for producing diluted vaccinia virus comprises the following steps:
step one, selecting male rabbits for passage;
step two, quarantine observation;
step three, inoculating the male rabbits;
step four, feeding and observing the inoculated male rabbits;
step five, taking testis of the male rabbit;
step six, the testicles are trimmed and washed;
seventhly, mashing the taken testis;
step eight, performing freeze thawing on the mashed testis;
step nine, centrifuging the frozen and thawed testicles;
step ten, subpackaging the centrifuged testis;
step eleven, diluting the vaccinia virus.
3. The method for producing diluted vaccinia virus-producing rabbit fur as claimed in claim 2, wherein the dilution of vaccinia virus is 1000-fold.
4. The method for producing diluted vaccinia virus-causing rabbit fur according to claim 1, wherein the diluted vaccinia virus fed for injection is used for observing pox-causing conditions: the number of the pox with the diameter of 1-2 cm is more than 80, and the total number of the pox with the diameter of less than 1cm is more than 100.
5. The method for producing diluted vaccinia virus inflammation-causing rabbit fur as claimed in claim 1, wherein the quick freezing temperature of the rabbit fur is-18 ℃, the rabbit fur is intact, reddish in color, without putrefactive odor, and the pox is purplish red.
6. The method for producing diluted vaccinia virus-causing rabbit skin according to claim 2, wherein the fifth step, the sixth step, the seventh step, the tenth step and the eleventh step are all performed inside a biosafety cabinet.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210878980.XA CN115212235A (en) | 2022-07-25 | 2022-07-25 | Production method of rabbit fur capable of causing inflammation by diluted vaccinia virus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210878980.XA CN115212235A (en) | 2022-07-25 | 2022-07-25 | Production method of rabbit fur capable of causing inflammation by diluted vaccinia virus |
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| Publication Number | Publication Date |
|---|---|
| CN115212235A true CN115212235A (en) | 2022-10-21 |
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| CN202210878980.XA Pending CN115212235A (en) | 2022-07-25 | 2022-07-25 | Production method of rabbit fur capable of causing inflammation by diluted vaccinia virus |
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6365192B1 (en) * | 1999-04-15 | 2002-04-02 | Nippon Zoki Pharmaceutical Co., Ltd. | Bioactivating substance |
| CN1493302A (en) * | 2002-10-31 | 2004-05-05 | 威世药业（如皋）有限公司 | Rabbit skin containing bioactive substance and its use |
| CN1613305A (en) * | 2004-12-06 | 2005-05-11 | 威世药业（如皋）有限公司 | Cony fur containing biological active substances and use thereof |
| CN111560318A (en) * | 2019-10-28 | 2020-08-21 | 威世药业（如皋）有限公司 | Freeze-drying process for vaccinia virus |
| CN111904981A (en) * | 2020-09-07 | 2020-11-10 | 威世药业（如皋）有限公司 | Production method of variola pox virus inflammation-causing rabbit fur |
-
2022
- 2022-07-25 CN CN202210878980.XA patent/CN115212235A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6365192B1 (en) * | 1999-04-15 | 2002-04-02 | Nippon Zoki Pharmaceutical Co., Ltd. | Bioactivating substance |
| CN1493302A (en) * | 2002-10-31 | 2004-05-05 | 威世药业（如皋）有限公司 | Rabbit skin containing bioactive substance and its use |
| US20060051375A1 (en) * | 2002-10-31 | 2006-03-09 | Cheung Wing S | Rabbit skin comprising biological active substances and its use |
| CN1613305A (en) * | 2004-12-06 | 2005-05-11 | 威世药业（如皋）有限公司 | Cony fur containing biological active substances and use thereof |
| CN111560318A (en) * | 2019-10-28 | 2020-08-21 | 威世药业（如皋）有限公司 | Freeze-drying process for vaccinia virus |
| CN111904981A (en) * | 2020-09-07 | 2020-11-10 | 威世药业（如皋）有限公司 | Production method of variola pox virus inflammation-causing rabbit fur |
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