CN115191518A - Method for preparing DHA algae oil powder feed additive by using fermentation filtrate - Google Patents

Method for preparing DHA algae oil powder feed additive by using fermentation filtrate Download PDF

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Publication number
CN115191518A
CN115191518A CN202210659005.XA CN202210659005A CN115191518A CN 115191518 A CN115191518 A CN 115191518A CN 202210659005 A CN202210659005 A CN 202210659005A CN 115191518 A CN115191518 A CN 115191518A
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dha
feed additive
fermentation filtrate
preparing
fermentation
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李伟鸿
钱洁
李培培
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Novosana Taicang Co ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/158—Fatty acids; Fats; Products containing oils or fats
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/10—Shaping or working-up of animal feeding-stuffs by agglomeration; by granulation, e.g. making powders
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K40/00—Shaping or working-up of animal feeding-stuffs
    • A23K40/30—Shaping or working-up of animal feeding-stuffs by encapsulating; by coating

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Physiology (AREA)
  • Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Fodder In General (AREA)
  • Feed For Specific Animals (AREA)

Abstract

The application discloses a method for preparing a DHA algae oil powder feed additive by using fermentation filtrate, which comprises the following steps: firstly, carrying out dehydration treatment on DHA algal oil fermentation filtrate to obtain concentrated solution; then emulsifying, homogenizing and embedding the concentrated solution, an embedding material and an emulsifying agent to obtain an emulsion; and finally, drying the emulsion to obtain the DHA algae oil powder feed additive. According to the application, the DHA algae oil fermentation filtrate is dehydrated to form concentrated solution, and then embedding materials are added for emulsification and homogenization, and then the concentrated solution is dried to form DHA algae oil powder which can be used as a feed additive to be added into animal feed; in addition, the DHA algae oil fermentation filtrate is recycled after dehydration, and the dehydrated water vapor hardly contains or contains few organic substance residues, so that the wastewater discharge qualification rate can be improved.

Description

Method for preparing DHA algae oil powder feed additive by using fermentation filtrate
Technical Field
The application relates to the field of biotechnology, in particular to a method for preparing a DHA algal oil powder feed additive by utilizing fermentation filtrate.
Background
DHA (docosahexaenoic acid), commonly known as NAOHUANG, is a polyunsaturated fatty acid which is very important to human body and belongs to an important member of Omega-3 unsaturated fatty acid family. DHA is not self-synthesized in the body and must be taken by food. It has the functions of strengthening intelligence, promoting brain cell development and curing angiocardiopathy and cerebrovascular disease, etc., and is known as new-generation functional health-care factor, so that it can be extensively used in the infant food additive and medicine industry.
At present, DHA grease mainly comes from fermentation production, and is obtained by carrying out enzymolysis and wall breaking on fermentation liquor and then centrifuging. However, in the above process, a large amount of fermentation filtrate is generated along with the separation and extraction of DHA oil, and a certain amount of DHA remains in the fermentation filtrate; if the fermentation filtrate is directly discharged, not only is the residual DHA wasted, but also the protein, glucose, bio-fiber, COD, BOD, and the like contained in the fermentation filtrate increase the wastewater treatment cost.
Disclosure of Invention
In view of the above problems, the application provides a method for preparing a DHA algae oil powder feed additive by using a fermentation filtrate, which comprises the steps of dehydrating the DHA algae oil fermentation filtrate to form a concentrated solution, subsequently adding an embedding material, emulsifying, homogenizing and drying to form DHA algae oil powder, wherein the DHA algae oil powder can be used as a feed additive to be added into animal feed, and the DHA algae oil fermentation filtrate is dehydrated and then recycled, wherein water vapor subjected to dehydration treatment almost contains no or very little organic matter residues, and the wastewater discharge qualification rate can be improved.
In order to achieve the above purpose, the present application provides the following technical solutions:
a method for preparing DHA algae oil powder feed additive by using fermentation filtrate comprises the following steps:
step one, carrying out dehydration treatment on DHA algae oil fermentation filtrate to obtain concentrated solution;
step two, emulsifying, homogenizing and embedding the concentrated solution, an embedding material and an emulsifier to obtain emulsion;
and step three, drying the emulsion to obtain the DHA algae oil powder feed additive.
Preferably, the DHA algal oil fermentation filtrate is obtained by the following steps:
firstly, inoculating a strain into a fermentation medium for fermentation after enlarged culture to obtain fermentation liquor;
secondly, adjusting the pH value of the fermentation liquor to be alkaline and heating;
thirdly, carrying out enzymolysis wall breaking on the fermentation liquor by using compound enzyme to obtain enzymolysis liquid;
fourthly, centrifuging the enzymolysis liquid in a nitrogen environment, and taking supernatant liquor;
and fifthly, extracting DHA grease from the supernatant to obtain the residual DHA algae oil fermentation filtrate.
Preferably, after the first step, the method further comprises: and step four, carrying out standardization treatment on the concentrated solution to enable DHA in the concentrated solution to reach a preset concentration.
Preferably, the predetermined concentration is 5% to 20%, and more preferably, the predetermined concentration is 10%.
Preferably, the embedding material comprises: at least one of starch, maltodextrin, and starch syrup dry powder.
Preferably, the emulsifier comprises: at least one of monoglyceride, sucrose fatty acid ester, and soybean phospholipid.
Preferably, the emulsification comprises: and (4) ultrasonic emulsification.
Preferably, homogenization comprises: shearing and homogenizing, or high-pressure homogenizing.
Preferably, after the second step, the method further comprises: and step five, concentrating the emulsion.
Preferably, the drying comprises: spray drying, or freeze drying.
The DHA algal oil fermentation filtrate is waste liquid remained after strain inoculation, fermentation, enzymolysis wall breaking and centrifugal extraction of DHA oil. The rest DHA algae oil fermentation filtrate generally contains more than 1% of DHA residue, the DHA algae oil fermentation filtrate is reprocessed and utilized, and the prepared DHA algae oil powder feed additive can be used as a feed additive for livestock, aquaculture animals or companion animals. The livestock comprises cattle, pig, chicken, duck, goose, etc., the aquaculture animals comprise fish, shrimp, etc., and the companion animals comprise cat, dog, etc.
The DHA content of the DHA algal oil fermentation filtrate can be improved through dehydration treatment. In order to accurately control the concentration of DHA, the concentrated solution is subjected to standardization treatment to obtain the concentrated solution with preset concentration.
Mixing the concentrated solution, embedding material and emulsifier, and emulsifying, homogenizing and embedding with a homogenizer under closed circulation condition to obtain emulsion.
Drying the emulsion to obtain DHA algae oil powder, and making full use of beneficial substances such as DHA, protein, glucose, biological fiber, etc. contained in DHA algae oil fermentation filtrate as feed additive to make animal feed contain more abundant nutritional components.
According to the application, the DHA algae oil fermentation filtrate is recycled after dehydration, and the dehydrated water vapor hardly contains or contains few organic matters, so that the wastewater discharge qualified rate can be improved.
Drawings
Various additional advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiments. The drawings are only for purposes of illustrating the preferred embodiments and are not to be construed as limiting the application. Also, like reference numerals are used to refer to like parts throughout the drawings. In the drawings:
FIG. 1 is a block flow diagram of a method for preparing a DHA algal oil meal feed additive using a fermentation filtrate according to the present application;
fig. 2 is a block flow diagram of another method for preparing DHA algal oil meal feed additive using fermentation filtrate according to the present application.
Detailed Description
The technical solutions of the present application will be described clearly and completely below with reference to embodiments of the present application, and it should be apparent that the described embodiments are only a part of the embodiments of the present application, and not all embodiments. All other embodiments obtained by a person of ordinary skill in the art based on the embodiments in the present application without making any creative effort belong to the protection scope of the present application.
Referring to fig. 1, a method for preparing DHA algal oil meal feed supplement using fermentation filtrate according to the present application is illustrated by examples 1 to 3.
Example 1
The method for preparing the DHA algae oil powder feed additive by utilizing the fermentation filtrate comprises the following steps:
step 1, carrying out dehydration treatment on DHA algal oil fermentation filtrate to obtain concentrated solution. The dehydration treatment may be performed by multiple effect evaporation cycles using steam.
And 2, mixing the concentrated solution with an embedding material and an emulsifying agent, and emulsifying, homogenizing and embedding the mixture by a homogenizer under a closed cycle condition to obtain an emulsion. Wherein the embedding material is starch, and the emulsifier is monoglyceride and diglyceride and sucrose fatty acid ester; the concentrated solution, the embedding material and the emulsifier comprise the following components in parts by mass: 30 parts of concentrated solution, 60 parts of embedding material and 10 parts of emulsifier. Wherein the emulsification is ultrasonic emulsification, and the ultrasonic frequency is 20KHz; homogenizing under high pressure of 20MPa.
And 3, drying the emulsion to obtain the DHA algae oil powder feed additive. The emulsion may be spray dried.
Example 2
The method for preparing the DHA algae oil powder feed additive by utilizing the fermentation filtrate comprises the following steps:
step 1, carrying out dehydration treatment on DHA algal oil fermentation filtrate to obtain concentrated solution. The dehydration treatment may be performed by multiple effect evaporation cycles using steam.
And 2, mixing the concentrated solution with an embedding material and an emulsifier, and emulsifying, homogenizing and embedding the mixture by a homogenizer under a closed cycle condition to obtain the emulsion. Wherein the embedding material is maltodextrin, and the emulsifier is soybean phospholipid; the concentrated solution, the embedding material and the emulsifier comprise the following components in parts by mass: 35 parts of concentrated solution, 50 parts of embedding material and 15 parts of emulsifier. Wherein the emulsification is ultrasonic emulsification, and the ultrasonic frequency is 30KHz; homogenization is shear homogenization, with a homogenization rate of 8000rpm.
And 3, drying the emulsion to obtain the DHA algae oil powder feed additive. The emulsion may be spray dried.
Example 3
The method for preparing the DHA algae oil powder feed additive by utilizing the fermentation filtrate comprises the following steps:
step 1, carrying out dehydration treatment on DHA algal oil fermentation filtrate to obtain concentrated solution. The dehydration treatment may be performed by using steam through a multi-effect evaporation cycle.
And 2, mixing the concentrated solution with an embedding material and an emulsifier, and emulsifying, homogenizing and embedding the mixture by a homogenizer under a closed cycle condition to obtain the emulsion. Wherein the embedding material is starch syrup dry powder, and the emulsifying agent is sucrose fatty acid ester and soybean phospholipid; the concentrated solution, the embedding material and the emulsifier comprise the following components in parts by mass: 40 parts of concentrated solution, 50 parts of embedding material and 10 parts of emulsifier. Wherein the emulsification is ultrasonic emulsification, and the ultrasonic frequency is 25KHz; homogenizing under high pressure of 55MPa.
And 3, drying the emulsion to obtain the DHA algae oil powder feed additive. The emulsion may be freeze-dried.
Example 4
The method for preparing the DHA algae oil powder feed additive by utilizing the fermentation filtrate comprises the following steps:
step 1, carrying out dehydration treatment on DHA algal oil fermentation filtrate to obtain concentrated solution. The dehydration treatment may be performed by multiple effect evaporation cycles using steam.
And 2, standardizing the concentrated solution to enable DHA in the concentrated solution to reach a preset concentration. Wherein the preset concentration is 5% -20%.
And 3, mixing the concentrated solution with an embedding material and an emulsifier, and emulsifying, homogenizing and embedding the mixture by a homogenizer under a closed cycle condition to obtain the emulsion. Wherein the embedding material is starch, and the emulsifier is monoglyceride and diglyceride and sucrose fatty acid ester; the concentrated solution, the embedding material and the emulsifier comprise the following components in parts by mass: 30 parts of concentrated solution, 60 parts of embedding material and 10 parts of emulsifier. Wherein the emulsification is ultrasonic emulsification, and the ultrasonic frequency is 20KHz; homogenizing under high pressure of 20MPa.
And 4, drying the emulsion to obtain the DHA algae oil powder feed additive. The emulsion may be spray dried.
Example 5
The method for preparing the DHA algae oil powder feed additive by utilizing the fermentation filtrate comprises the following steps:
step 1, carrying out dehydration treatment on DHA algal oil fermentation filtrate to obtain concentrated solution. The dehydration treatment may be performed by using steam through a multi-effect evaporation cycle.
And 2, carrying out standardized treatment on the concentrated solution to enable DHA in the concentrated solution to reach a preset concentration. Wherein the preset concentration is 20%.
And 3, mixing the concentrated solution with an embedding material and an emulsifier, and emulsifying, homogenizing and embedding the mixture by a homogenizer under a closed cycle condition to obtain the emulsion. Wherein the embedding material is maltodextrin, and the emulsifier is soybean phospholipid; the concentrated solution, the embedding material and the emulsifier comprise the following components in parts by mass: 35 parts of concentrated solution, 50 parts of embedding material and 15 parts of emulsifier. Wherein the emulsification is ultrasonic emulsification, and the ultrasonic frequency is 30KHz; homogenization is shear homogenization, with a homogenization rate of 15000rpm.
And 4, drying the emulsion to obtain the DHA algae oil powder feed additive. The emulsion may be spray dried.
Example 6
The method for preparing the DHA algae oil powder feed additive by utilizing the fermentation filtrate comprises the following steps:
step 1, carrying out dehydration treatment on DHA algal oil fermentation filtrate to obtain concentrated solution. The dehydration treatment may be performed by using steam through a multi-effect evaporation cycle.
And 2, mixing the concentrated solution with an embedding material and an emulsifier, and emulsifying, homogenizing and embedding the mixture by a homogenizer under a closed cycle condition to obtain the emulsion. Wherein the embedding material is starch syrup dry powder, and the emulsifier is sucrose fatty acid ester and soybean phospholipid; the concentrated solution, the embedding material and the emulsifier comprise the following components in parts by mass: 40 parts of concentrated solution, 50 parts of embedding material and 10 parts of emulsifier. Wherein the emulsification is ultrasonic emulsification, and the ultrasonic frequency is 25KHz; homogenizing under 55MPa.
And 3, concentrating the emulsion. The high pressure homogeneous emulsion was concentrated under reduced pressure.
And 4, drying the emulsion to obtain the DHA algae oil powder feed additive. The emulsion may be freeze-dried.
Referring to fig. 2, a method for preparing DHA algal oil meal feed supplement using fermentation filtrate according to the present application is illustrated by example 7.
Example 7
The method for preparing the DHA algae oil powder feed additive by utilizing the fermentation filtrate comprises the following steps:
step 1, inoculating the strain into a fermentation culture medium for fermentation after amplification culture to obtain fermentation liquor. Wherein the strain is Schizochytrium limacinum, and the culture medium is glucose.
And 2, adjusting the pH value of the fermentation liquor to be alkaline and heating. Wherein the pH value of the fermentation liquor is 7.5-9, and the temperature rise is 50-60 ℃.
And 3, carrying out enzymolysis wall breaking on the fermentation liquor by using the complex enzyme to obtain an enzymolysis liquid. Wherein the complex enzyme is a mixture of alkaline protease and lipase.
And 4, centrifuging the enzymolysis liquid in a nitrogen environment, and taking supernatant liquor. Wherein, the rotating speed is 8000rpm.
And 5, extracting DHA grease from the supernatant to obtain the residual DHA algal oil fermentation filtrate. The DHA oil can be extracted by removing the water in the supernatant, and the wastewater is the residual DHA algae oil fermentation filtrate.
And 6, carrying out dehydration treatment on the DHA algal oil fermentation filtrate by multi-effect evaporation and recycling steam to obtain a concentrated solution.
And 7, standardizing the concentrated solution to enable the concentration of DHA in the concentrated solution to reach 10%.
And 8, mixing 30 parts by mass of the concentrated solution with 60 parts by mass of embedding material (starch) and 10 parts by mass of emulsifier (monoglyceride and diglyceride and sucrose fatty acid ester), and performing ultrasonic emulsification, high-pressure homogenization and embedding by using a homogenizer under a closed cycle condition to obtain an emulsion. Wherein the ultrasonic frequency is 25KHz, and the homogenizing pressure is 55MPa.
And 9, carrying out reduced pressure concentration treatment on the high-pressure homogenized emulsion.
And step 10, carrying out spray drying on the emulsion to obtain the DHA algae oil powder feed additive.
It should be noted that the above-mentioned embodiments illustrate rather than limit the application, and that those skilled in the art will be able to design alternative embodiments without departing from the scope of the appended claims.

Claims (10)

1. A method for preparing a DHA algae oil powder feed additive by using fermentation filtrate is characterized by comprising the following steps:
step one, carrying out dehydration treatment on DHA algal oil fermentation filtrate to obtain a concentrated solution;
step two, emulsifying, homogenizing and embedding the concentrated solution, an embedding material and an emulsifier to obtain emulsion;
and step three, drying the emulsion to obtain the DHA algae oil powder feed additive.
2. The method for preparing a DHA algal oil meal feed additive by using fermentation filtrate according to claim 1, wherein the DHA algal oil fermentation filtrate is obtained by the following steps:
firstly, inoculating a strain into a fermentation medium for fermentation after enlarged culture to obtain fermentation liquor;
secondly, adjusting the pH value of the fermentation liquor to be alkaline and heating;
thirdly, carrying out enzymolysis and wall breaking on the fermentation liquor by using compound enzyme to obtain enzymolysis liquid;
fourthly, centrifuging the enzymolysis liquid in a nitrogen environment, and taking supernatant liquor;
and fifthly, extracting DHA grease from the supernatant to obtain the residual DHA algae oil fermentation filtrate.
3. The method for preparing DHA algal oil meal feed additive according to the fermentation filtrate of claim 1, wherein after the first step, the method further comprises:
and step four, carrying out standardization treatment on the concentrated solution to enable DHA in the concentrated solution to reach a preset concentration.
4. The method for preparing a DHA algal oil meal feed additive by using fermentation filtrate according to claim 3, wherein the predetermined concentration is 5% -20%.
5. The method for preparing DHA algal oil meal feed additive according to claim 1, wherein the embedding material comprises: at least one of starch, maltodextrin, and starch syrup dry powder.
6. The method for preparing DHA algal oil meal feed additive according to claim 1, wherein the emulsifier comprises: at least one of monoglyceride, sucrose fatty acid ester, and soybean phospholipid.
7. The method for preparing DHA algal oil meal feed additive from fermentation filtrate according to claim 1, wherein the emulsifying comprises: and (4) ultrasonic emulsification.
8. The method for preparing a DHA algal oil meal feed additive of claim 1, wherein the homogenizing comprises: shearing and homogenizing, or high-pressure homogenizing.
9. The method for preparing DHA algal oil meal feed additive according to the fermentation filtrate of claim 1, wherein after the second step, the method further comprises:
and step five, concentrating the emulsion.
10. The method for preparing a DHA algal oil meal feed additive from fermentation filtrate according to claim 1, wherein the drying includes: spray drying, or freeze drying.
CN202210659005.XA 2022-06-09 2022-06-09 Method for preparing DHA algae oil powder feed additive by using fermentation filtrate Pending CN115191518A (en)

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Citations (6)

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Publication number Priority date Publication date Assignee Title
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CN102919512A (en) * 2012-09-26 2013-02-13 内蒙古金达威药业有限公司 DHA (docosahexaenoic acid)-rich microalgae powder and preparation method thereof
CN103820326A (en) * 2014-03-20 2014-05-28 青岛琅琊台集团股份有限公司 Method for producing dry algae powder by virtue of cultivation of ocean microalgae
CN108559615A (en) * 2018-05-04 2018-09-21 南京工业大学 Method for comprehensively utilizing DHA (docosahexaenoic acid) fermented wastewater/algae residues
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Patent Citations (6)

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Publication number Priority date Publication date Assignee Title
CN101999522A (en) * 2010-09-10 2011-04-06 厦门汇盛生物有限公司 Microalgae whole cell powder for making mammals produce DHA milk in high yield and preparation method thereof
CN102919512A (en) * 2012-09-26 2013-02-13 内蒙古金达威药业有限公司 DHA (docosahexaenoic acid)-rich microalgae powder and preparation method thereof
CN103820326A (en) * 2014-03-20 2014-05-28 青岛琅琊台集团股份有限公司 Method for producing dry algae powder by virtue of cultivation of ocean microalgae
CN108559615A (en) * 2018-05-04 2018-09-21 南京工业大学 Method for comprehensively utilizing DHA (docosahexaenoic acid) fermented wastewater/algae residues
CN111919909A (en) * 2020-08-16 2020-11-13 烟台大学 Algae oil microcapsule powder and preparation method thereof
CN114041601A (en) * 2021-12-06 2022-02-15 润科生物工程（福建）有限公司 Process for preparing DHA microcapsule powder

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