CN108559615A - Method for comprehensively utilizing DHA (docosahexaenoic acid) fermented wastewater/algae residues - Google Patents
Method for comprehensively utilizing DHA (docosahexaenoic acid) fermented wastewater/algae residues Download PDFInfo
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- 241000195493 Cryptophyta Species 0.000 title abstract 3
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- 235000020669 docosahexaenoic acid Nutrition 0.000 title description 28
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- 238000000855 fermentation Methods 0.000 claims abstract description 49
- 230000004151 fermentation Effects 0.000 claims abstract description 49
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 42
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- 108010014251 Muramidase Proteins 0.000 claims description 2
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 claims description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 2
- 238000000265 homogenisation Methods 0.000 claims description 2
- 229960000274 lysozyme Drugs 0.000 claims description 2
- 239000004325 lysozyme Substances 0.000 claims description 2
- 235000010335 lysozyme Nutrition 0.000 claims description 2
- 238000001694 spray drying Methods 0.000 claims description 2
- 241000219112 Cucumis Species 0.000 claims 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 claims 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 claims 1
- 239000002023 wood Substances 0.000 claims 1
- 238000000605 extraction Methods 0.000 abstract description 17
- 229940041514 candida albicans extract Drugs 0.000 abstract description 7
- 239000012138 yeast extract Substances 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000003912 environmental pollution Methods 0.000 abstract description 4
- 210000002421 cell wall Anatomy 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 13
- 235000019198 oils Nutrition 0.000 description 11
- 241000003595 Aurantiochytrium limacinum Species 0.000 description 9
- 239000004615 ingredient Substances 0.000 description 8
- 239000002028 Biomass Substances 0.000 description 6
- 239000012530 fluid Substances 0.000 description 6
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- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
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- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
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- 230000015572 biosynthetic process Effects 0.000 description 2
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- 235000008504 concentrate Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000021323 fish oil Nutrition 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
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- DVSZKTAMJJTWFG-SKCDLICFSA-N (2e,4e,6e,8e,10e,12e)-docosa-2,4,6,8,10,12-hexaenoic acid Chemical compound CCCCCCCCC\C=C\C=C\C=C\C=C\C=C\C=C\C(O)=O DVSZKTAMJJTWFG-SKCDLICFSA-N 0.000 description 1
- GZJLLYHBALOKEX-UHFFFAOYSA-N 6-Ketone, O18-Me-Ussuriedine Natural products CC=CCC=CCC=CCC=CCC=CCC=CCCCC(O)=O GZJLLYHBALOKEX-UHFFFAOYSA-N 0.000 description 1
- 241000251468 Actinopterygii Species 0.000 description 1
- 244000189799 Asimina triloba Species 0.000 description 1
- 235000006264 Asimina triloba Nutrition 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- 241000233652 Chytridiomycota Species 0.000 description 1
- 241001191009 Gymnomyza Species 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 239000007832 Na2SO4 Substances 0.000 description 1
- 108090000526 Papain Proteins 0.000 description 1
- 101710093543 Probable non-specific lipid-transfer protein Proteins 0.000 description 1
- 241000598397 Schizochytrium sp. Species 0.000 description 1
- 241000986329 Schizochytrium sp. CCTCC M209059 Species 0.000 description 1
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 1
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 1
- 210000003719 b-lymphocyte Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000023555 blood coagulation Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 230000006037 cell lysis Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 235000005911 diet Nutrition 0.000 description 1
- 230000037213 diet Effects 0.000 description 1
- KAUVQQXNCKESLC-UHFFFAOYSA-N docosahexaenoic acid (DHA) Natural products COC(=O)C(C)NOCC1=CC=CC=C1 KAUVQQXNCKESLC-UHFFFAOYSA-N 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000839 emulsion Substances 0.000 description 1
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- 235000013305 food Nutrition 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 235000014666 liquid concentrate Nutrition 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
- 229940055729 papain Drugs 0.000 description 1
- 235000019834 papain Nutrition 0.000 description 1
- 235000021085 polyunsaturated fats Nutrition 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
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- 230000001932 seasonal effect Effects 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052938 sodium sulfate Inorganic materials 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 238000010025 steaming Methods 0.000 description 1
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- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B1/00—Production of fats or fatty oils from raw materials
- C11B1/02—Pretreatment
- C11B1/025—Pretreatment by enzymes or microorganisms, living or dead
-
- C—CHEMISTRY; METALLURGY
- C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
- C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
- C11B3/00—Refining fats or fatty oils
- C11B3/16—Refining fats or fatty oils by mechanical means
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Mechanical Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention discloses a method for comprehensively utilizing wastewater and waste residues generated by DHA fermentation. The method comprises the following specific steps: concentrating the fermentation liquor after fermentation is finished, and separating wastewater; breaking cell wall, extracting oil and fat, collecting algae residue, and concentrating (or not concentrating) algae residue and waste water for reuse. The invention takes the extraction of DHA grease and the pretreatment of fermentation wastewater and waste residue into consideration, realizes the high-efficiency extraction of grease, recycles wastewater and waste residue, reduces the discharge of wastewater and waste residue, saves the production cost and greatly reduces the environmental pollution. The method can realize the extraction rate of the grease above 85%; the waste water can partially replace pure water, and the waste residue is an efficient nitrogen source and can partially or completely replace yeast extract.
Description
Technical field
The present invention is field of microbial fermentation, in particular for the extraction of intracellular product, by the broken wall and DHA grease of cell
Extraction be combined, in the technique in downstream realize waste water and dregs pretreatment and grease extraction.
Background technology
In recent years, the important function due to polyunsaturated fatty acid in health was increasingly by the extensive pass of people
Note.In numerous unsaturated fatty acids, it is polyunsaturated fat that docosahexaenoic acid (DHA), which is a kind of important ω -3,
Acid can promote the development of brain and retina, do the functions such as blood coagulation, reducing blood lipid.But DHA cannot be synthesized by human body, Zhi Nengcong
It is obtained in diet, to meet nutritional need.Traditional DHA is mainly derived from deep sea fish oil, these fishes can accumulate the original of DHA oil
It is food because being them with the microalgae that can generate DHA in ocean in cell.Since traditional deep sea fish oil is compared with microalgae grease
Have the shortcomings that very much, including the reasons such as environmental pollution, Accumulation of heavy metals and seasonal variations, microalgae DHA grease be more and more
It is valued by people.Currently, having proven to schizochytrium limacinum (schizochytrium sp.) because of grease and DHA content height, grease
Do not have fishlike smell, to person poultry harmless and be easy to culture etc. advantages, be DHA production ideal commercial strain.
Microalgae DHA productions, belong to microorganism fluid fermentation arts, unavoidably will produce fermentation waste water and waste residue, wherein
The mineral ions and acid ion that waste water contains a large amount of moisture and is not used by, this is important to maintaining the infiltration of zymotic fluid to be pressed with
Effect, can be as the substitute of pure water；Waste residue contains the ingredients such as a large amount of protein, polypeptide, carbohydrate, other function factors,
A kind of novel nitrogen source, the substitute as nitrogen substance in fermentation medium can be used as.Meanwhile DHA is produced as a kind of intracellular
Object needs the broken wall for carrying out cell in oil extraction processes, can also be related to cell for the smaller fermentation system of biomass
Concentration.Therefore, the pretreatment that the collection and waste residue of fermentation waste water are integrated in the oil extraction processes of downstream, to realize grease height
Imitate green production, application to waste and reducing environmental pollution is of great significance.
Invention content
The present invention is directed to comprehensive downstream grease extraction operations, and the extraction of grease is combined with the pretreatment of waste, was both carried
The extraction efficiency of high grease, and waste reclamation can will be generated in oil extraction processes.
The specific technical solution of the present invention is：
A kind of method of the waste water algae-residue comprehensive utilization of DHA fermentations, wherein this method is useless containing fermentation is obtained after fermentation
The step of water and/or fermentation algae-residue and reuse, and use physics centrifugation side during obtaining fermentation waste water and/or fermentation algae-residue
Method collects grease.
Method of the present invention, wherein the method for obtaining fermentation waste water is, after fermentation, using natural subsidence,
The method of centrifugation or addition external source sedimentation agent detaches waste water and thalline, obtains Fermented Condensed liquid and waste water.
Method of the present invention, wherein the method for obtaining fermentation algae-residue is that, after fermentation, separation is obtained containing useless
The Fermented Condensed liquid of water and mycetome, and concentrate is subjected to broken wall；Bacterium solution after broken wall is heated to 95-98 DEG C, and protects
It holds and is centrifuged at this temperature；Oil phase A and aqueous phase B are obtained, wherein aqueous phase B is the hydrolyzate containing algae-residue, by hydrolyzate
Concentration obtains algae-residue.
Method of the present invention, wherein the wall-breaking method is enzymatic shell-broken, acid system broken wall, liquid nitrogen broken wall and high pressure
One kind in homogenate method broken wall.
Method of the present invention, wherein enzyme used in the enzymatic shell-broken is alkali protease, papain, molten
Or mixtures thereof one kind of bacterium enzyme and glusulase.
Method of the present invention, wherein the method for the concentration is that distillation, vacuum rotary steam, freeze-drying or spraying are dry
One kind in dry.
Method of the present invention, wherein the algae-residue nitrogen content (being in terms of 50% by water content after concentration) is total weight
0.5-5.5%, the 2-8% that ammonia nitrogen is the 0.01-0.05% of total weight, carbohydrate is total weight.
Method of the present invention, wherein it is 0%-60% that the waste water substitutes pure water rate in the medium.
Method of the present invention, wherein the waste residue is in culture medium as nitrogen source substitute, substitutive medium nitrogen pool
0%-100% substitute
Method of the present invention, wherein the waste water and waste residue may be used alone or in combination.
In method of the present invention, a preferred embodiment is：
Using schizochytrium limacinum as fermentation strain, after seed culture, fermented and cultured is carried out in fermentation tank, after fermentation
Zymotic fluid be process object involved by the invention.Its specific steps are：
A. the acquisition of fermentation waste water：It will be fermented using centrifugation or natural subsidence or the method for adding sedimentation agent after fermentation
Liquid concentrates, and it is fermentation waste water of the present invention to collect the waste water after centrifugation or the supernatant after natural subsidence.Distinguishingly, this hair
It is bright that toxic action should must not be played to the growth and breeding of cell using addition sedimentation agent.
B. the broken wall of cell：After the concentration that step A is obtained zymotic fluid carry out broken wall, wall-breaking method can be enzymatic shell-broken,
Acid system broken wall, liquid nitrogen broken wall and high-pressure homogenization broken wall.Wherein use the enzyme used in enzymatic shell-broken for alkali protease, pawpaw egg
Or mixtures thereof one kind of white enzyme, lysozyme and glusulase；Acid used in acid system broken wall is hydrochloric acid or sulfuric acid；Liquid nitrogen broken wall and height
Pressure homogenate method broken wall does not have particular/special requirement.Distinguishingly, higher in cell concentration, A processing steps can not be used, directly
The broken wall that the zymotic fluid after fermentation is carried out to step B cell is connect, in this way, not just being related to the reuse of fermentation waste water.
The extraction of C.DHA greases and the acquisition of algae-residue：Bacterium solution after step B broken walls is heated to 95-98 DEG C, to eliminate
Emulsion is then maintained at the extraction for using three-phase centrifuge to carry out grease at a temperature of this.It is DHA maos wherein to collect oil phase
Oil；Water phase is collected, is algae-residue hydrolyzate, contains the nutrition such as abundant protein, polypeptide, carbohydrate, other function factors in the phase
Ingredient.
D. algae-residue hydrolyzate concentrates：The algae-residue hydrolyzate that step C is obtained can directly use or concentrated without concentration
After use.The method of concentration can be that distillation, vacuum rotary steam, freeze-drying or spray drying obtain.Wherein, distill and depressurize rotation
The water of steaming can be used for fermenting required pure water.
E. the reuse of fermentation waste water and algae-residue.The algae-residue hydrolyzate that the waste water and step C or D that step A is collected are collected can be with
It, can be respectively according to the 0%- of the 0%-60% of water consumption and culture medium nitrogen pool as the substitute of fresh water and nitrogen source ingredient
100% replacement is used alone or be used in mixed way.
The acquisition methods of above-described grease extraction and fermentation waste water, waste residue are the processing methods of a set of synthesis, simultaneous
Concentration and the broken wall for having cared for cell are collected simultaneously the nutritional ingredient of algae-residue after waste water and broken wall after fermentation, can be recycled
Using.Salt and acid ion in fermentation waste water provide certain osmotic pressure for the growth of cell, are suitble to halophilic split
Grow the growth of chytrid；For fermentation residue because of breaking-wall cell and hydrolysis, free or hydrolysis intracellular nutritional ingredient is same to adapt to
It is utilized in the growth of schizochytrium limacinum.And growth and the DHA of schizochytrium limacinum cell are not influenced under the conditions of suitable adding proportion
Synthesis, even better than former culture medium.
The beneficial effects of the present invention are：
In entire oil extraction processes, required operating procedure is doubled as to the preprocessing means of latter operation, such as this hair
The concentration of bright involved cell is pre- as the hydrolysis of cytotrophy ingredient as the means of acquisition fermentation waste water, the broken wall of cell
Processing, the collection of grease being collected by centrifugation as algae-residue hydrolyzate.The present invention provides it is a kind of green, environmental protection DHA extraction and
Waste water and dregs recycle technique, reduce production cost and waste processing cost, and reduce environmental pollution.
Schizochytrium limacinum fermentation mistake is met with the ratio reuse of optimization using the fermentation waste water and waste residue acquired in the present invention
The nutritional condition of journey can promote the growth of cell and the accumulation of DHA, and due to easy to operate, low for equipment requirements, adapt to
Industrialized production.
Specific implementation mode
Embodiment 1
1 the present embodiment of embodiment is the bacterial strain and fermentation culture medium used in the present invention
The schizochytrium limacinum that bacterial strain uses is Schizochytrium sp.hx-308, is to be screened by Nanjing University of Technology, is excellent
Change simultaneously preservation, preservation place is another Type Tissue Collection (CCTCC), and deposit number is CCTCC M 209059.
Fermentation medium used is：Anhydrous Na2SO412-24g/L, MgSO4·7H2O 1-5g/L, (NH4)2SO4 2-
6g/L, KCl 1-3g/L, K2SO40.5-2.0g/L, CaCl20.1-0.5g/L, KH2PO41-4g/L, yeast extract 4-8g/L, just
Beginning glucose 20-60g/L, and when concentration of glucose is less than 5g/L by adding glucose solution (concentration of glucose 60-
80%) it is 1-5g/L to keep the concentration of glucose in zymotic fluid, until (entire fermentation process is that 100-140 is small to fermentation ends
When).
2 the present embodiment of embodiment is the analysis of fermentation waste water and waste residue nutritional ingredient
It on the basis of embodiment 1, ferments 120 hours, fermentation waste water is obtained using the method for centrifugation, using basic protein
Enzyme carries out broken wall to cell, DHA grease and hydrolysis algae-residue is collected after centrifugation through three-phase centrifuge, water phase algae-residue uses vacuum rotary steam
Method obtain concentration algae-residue.It is as shown in Table 1 and Table 2 to the analysis of the fermentation waste water and concentration algae-residue that are obtained.
Table 1：The ion component and content of fermentation waste water
Table 2：The total nitrogen and amino acid of waste residue form and the comparison of content and yeast extract
As shown in table 1, abundant mineral element and acid ion are contained in fermentation waste water, to maintaining the infiltration of fermentation process
It is pressed with important role.As shown in table 2, in waste residue rich content aminoacid ingredient, total nitrogen content reached 3.45%, though
Right total nitrogen content is less than traditional yeast extract, and waste residue can be added by the nitrogen pool needed for culture medium in practical application.
Influence of the adding proportion of the different waste water of embodiment 3 to biomass, oil content and DHA content
On the basis of embodiment 1, replaces the ratio of pure water to prepare fermentation medium with different fermentation waste waters and sent out
Ferment culture, fermentation results are listed in Table 3 below.
Influence of the 3 different fermentations waste water ratio of table to fermentation
As shown in table 3, fermentation waste water can with partial alternative pure water without influence schizochytrium limacinum growth and DHA accumulation,
But the higher fermentation substituted than being unfavorable for DHA, in short, 40% fermentation below substituted than not influencing schizochytrium limacinum DHA.
Influence of the adding proportion of the different waste residues of embodiment 4 to biomass, oil content and DHA content
On the basis of embodiment 1, it is added according to the nitrogen content of used yeast extract with different waste residues, such as table
Shown in 4, the usage amount of control group yeast extract is 4g/L.It is on this basis 4g/L × 10.16%=according to nitrogen pool
0.4064g/L prepares fermentation medium and carries out fermented and cultured, and fermentation results are listed in Table 5 below.
Table 4：Yeast extract and algae-residue difference adding proportion
Table 5：Influence of the different waste residue ratios to fermentation
As shown in table 5, the addition of waste residue does not influence schizochytrium limacinum biomass and the accumulation of DHA, but algae-residue replacing whole ferment
Female cream, biomass and fat content decrease.
The influence of 5 waste water of embodiment and waste residue mixing addition to biomass, oil content and DHA content
On the basis of embodiment 3 and embodiment 4, prepared using different waste water additive amounts and different waste residue additive amounts
Fermentation medium carries out fermented and cultured, and fermentation results are listed in Table 6 below.
Influence of the fermentation waste water and waste residue of 6 different proportion of table to fermentation
Claims (10)
1. a kind of method of the waste water algae-residue comprehensive utilization of DHA fermentations, which is characterized in that this method is containing acquisition hair after fermentation
Ferment waste water and/or fermentation algae-residue and the step of reuse, and during obtaining fermentation waste water and/or fermentation algae-residue using physics from
Heart method collects grease.
2. according to the method described in claim 1, it is characterized in that, the method for obtaining fermentation waste water is, after fermentation, to adopt
Waste water and thalline are detached with the method for natural subsidence, centrifugation or addition external source sedimentation agent, obtain Fermented Condensed liquid and waste water.
3. according to the method described in claim 1, it is characterized in that, the method for acquisition fermentation algae-residue is, after fermentation, to divide
From Fermented Condensed liquid of the acquisition containing waste water and mycetome, and concentrate is subjected to broken wall；Bacterium solution after broken wall is heated to 95-
98 DEG C, and keep being centrifuged at this temperature；Oil phase A and aqueous phase B are obtained, wherein aqueous phase B is the hydrolyzate containing algae-residue,
Hydrolyzate is concentrated and obtains algae-residue.
4. according to the method described in claim 3, it is characterized in that, the wall-breaking method is enzymatic shell-broken, acid system broken wall, liquid nitrogen
One kind in broken wall and high-pressure homogenization broken wall.
5. according to the method described in claim 4, it is characterized in that, the enzyme used in the enzymatic shell-broken is alkali protease, wood
Or mixtures thereof one kind of melon protease, lysozyme and glusulase.
6. according to the method described in claim 3, it is characterized in that, the method for the concentration is distillation, vacuum rotary steam, freezes and do
One kind in dry or spray drying.
7. according to the method described in claim 1, it is characterized in that, the algae-residue nitrogen content（With water content after concentration for 50%
Meter）The 2-8% that 0.5-5.5%, ammonia nitrogen for total weight are the 0.01-0.05% of total weight, carbohydrate is total weight.
8. according to the method described in claim 1, it is characterized in that, the waste water substitutes pure water in the medium, it is instead of rate
0%-60%。
9. according to the method described in claim 1, it is characterized in that, the waste residue in culture medium as nitrogen source substitute, substitute
The 0%-100% of culture medium nitrogen pool.
10. according to the method described in claim 1, it is characterized in that, the waste water and waste residue can be used alone or mix makes
With.
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| CN115191518A (en) * | 2022-06-09 | 2022-10-18 | 挪亚圣诺（太仓）生物科技有限公司 | Method for preparing DHA algae oil powder feed additive by using fermentation filtrate |
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