CN115191449A - 一种吉龙草提取物在防治南方根结线虫中的应用 - Google Patents
一种吉龙草提取物在防治南方根结线虫中的应用 Download PDFInfo
- Publication number
- CN115191449A CN115191449A CN202210941695.8A CN202210941695A CN115191449A CN 115191449 A CN115191449 A CN 115191449A CN 202210941695 A CN202210941695 A CN 202210941695A CN 115191449 A CN115191449 A CN 115191449A
- Authority
- CN
- China
- Prior art keywords
- extract
- meloidogyne incognita
- agrimonia pilosa
- application
- insecticidal
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000000284 extract Substances 0.000 title claims abstract description 42
- 241000243786 Meloidogyne incognita Species 0.000 title claims abstract description 34
- 241001278836 Agrimonia pilosa Species 0.000 claims abstract description 14
- 235000000641 Agrimonia pilosa Nutrition 0.000 claims abstract description 14
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 14
- 238000002360 preparation method Methods 0.000 claims abstract description 12
- 230000000694 effects Effects 0.000 claims abstract description 11
- 230000002265 prevention Effects 0.000 claims abstract description 10
- 241000238631 Hexapoda Species 0.000 claims abstract description 5
- 239000000203 mixture Substances 0.000 claims abstract description 4
- 239000002270 dispersing agent Substances 0.000 claims abstract 3
- 239000000575 pesticide Substances 0.000 claims abstract 3
- 239000000080 wetting agent Substances 0.000 claims abstract 3
- 239000000706 filtrate Substances 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 244000307697 Agrimonia eupatoria Species 0.000 claims description 4
- 241000607479 Yersinia pestis Species 0.000 claims description 4
- 235000000125 common agrimony Nutrition 0.000 claims description 4
- 239000000843 powder Substances 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- 240000008067 Cucumis sativus Species 0.000 claims description 2
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 2
- 235000007688 Lycopersicon esculentum Nutrition 0.000 claims description 2
- 240000003768 Solanum lycopersicum Species 0.000 claims description 2
- 244000061458 Solanum melongena Species 0.000 claims description 2
- 235000002597 Solanum melongena Nutrition 0.000 claims description 2
- 244000061456 Solanum tuberosum Species 0.000 claims description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 2
- 239000002689 soil Substances 0.000 claims description 2
- 230000000749 insecticidal effect Effects 0.000 claims 6
- 238000005507 spraying Methods 0.000 claims 2
- 244000144730 Amygdalus persica Species 0.000 claims 1
- 244000068988 Glycine max Species 0.000 claims 1
- 235000010469 Glycine max Nutrition 0.000 claims 1
- 235000006040 Prunus persica var persica Nutrition 0.000 claims 1
- 235000009754 Vitis X bourquina Nutrition 0.000 claims 1
- 235000012333 Vitis X labruscana Nutrition 0.000 claims 1
- 240000006365 Vitis vinifera Species 0.000 claims 1
- 235000014787 Vitis vinifera Nutrition 0.000 claims 1
- 244000273928 Zingiber officinale Species 0.000 claims 1
- 235000006886 Zingiber officinale Nutrition 0.000 claims 1
- 238000001035 drying Methods 0.000 claims 1
- 238000011010 flushing procedure Methods 0.000 claims 1
- 235000008397 ginger Nutrition 0.000 claims 1
- 239000002917 insecticide Substances 0.000 claims 1
- 239000000463 material Substances 0.000 claims 1
- 241000196324 Embryophyta Species 0.000 abstract description 20
- 230000002147 killing effect Effects 0.000 abstract description 8
- 238000011161 development Methods 0.000 abstract description 6
- 241000735332 Gerbera Species 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 5
- 239000002994 raw material Substances 0.000 abstract description 4
- 206010059866 Drug resistance Diseases 0.000 abstract description 2
- 230000009286 beneficial effect Effects 0.000 abstract description 2
- 241000244206 Nematoda Species 0.000 description 18
- 239000000243 solution Substances 0.000 description 13
- 229920001213 Polysorbate 20 Polymers 0.000 description 6
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 description 6
- 241001312741 Gekko swinhonis Species 0.000 description 5
- 238000002386 leaching Methods 0.000 description 5
- 230000003071 parasitic effect Effects 0.000 description 5
- 244000061176 Nicotiana tabacum Species 0.000 description 4
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 235000013399 edible fruits Nutrition 0.000 description 4
- 230000007613 environmental effect Effects 0.000 description 4
- 238000007873 sieving Methods 0.000 description 4
- 244000016281 Cymbalaria muralis Species 0.000 description 3
- 235000015916 Hydrocotyle vulgaris Nutrition 0.000 description 3
- 241000243785 Meloidogyne javanica Species 0.000 description 3
- 240000003705 Senecio vulgaris Species 0.000 description 3
- 241001506766 Xanthium Species 0.000 description 3
- 238000007865 diluting Methods 0.000 description 3
- 231100000225 lethality Toxicity 0.000 description 3
- 239000013642 negative control Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 239000002904 solvent Substances 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- 101800004637 Communis Proteins 0.000 description 2
- ULDHMXUKGWMISQ-UHFFFAOYSA-N carvone Chemical compound CC(=C)C1CC=C(C)C(=O)C1 ULDHMXUKGWMISQ-UHFFFAOYSA-N 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000001069 nematicidal effect Effects 0.000 description 2
- 239000005645 nematicide Substances 0.000 description 2
- 239000000419 plant extract Substances 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 238000000967 suction filtration Methods 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- WTARULDDTDQWMU-RKDXNWHRSA-N (+)-β-pinene Chemical compound C1[C@H]2C(C)(C)[C@@H]1CCC2=C WTARULDDTDQWMU-RKDXNWHRSA-N 0.000 description 1
- WTARULDDTDQWMU-IUCAKERBSA-N (-)-Nopinene Natural products C1[C@@H]2C(C)(C)[C@H]1CCC2=C WTARULDDTDQWMU-IUCAKERBSA-N 0.000 description 1
- 235000003092 Artemisia dracunculus Nutrition 0.000 description 1
- 240000001851 Artemisia dracunculus Species 0.000 description 1
- 235000000832 Ayote Nutrition 0.000 description 1
- 239000005973 Carvone Substances 0.000 description 1
- WTEVQBCEXWBHNA-UHFFFAOYSA-N Citral Natural products CC(C)=CCCC(C)=CC=O WTEVQBCEXWBHNA-UHFFFAOYSA-N 0.000 description 1
- 208000035473 Communicable disease Diseases 0.000 description 1
- 240000004244 Cucurbita moschata Species 0.000 description 1
- 235000009854 Cucurbita moschata Nutrition 0.000 description 1
- 235000009804 Cucurbita pepo subsp pepo Nutrition 0.000 description 1
- 241000234653 Cyperus Species 0.000 description 1
- 239000004278 EU approved seasoning Substances 0.000 description 1
- 241000131458 Elsholtzia Species 0.000 description 1
- 241000208152 Geranium Species 0.000 description 1
- 206010019233 Headaches Diseases 0.000 description 1
- 241000207923 Lamiaceae Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- WTARULDDTDQWMU-UHFFFAOYSA-N Pseudopinene Natural products C1C2C(C)(C)C1CCC2=C WTARULDDTDQWMU-UHFFFAOYSA-N 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 241000270295 Serpentes Species 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- XCPQUQHBVVXMRQ-UHFFFAOYSA-N alpha-Fenchene Natural products C1CC2C(=C)CC1C2(C)C XCPQUQHBVVXMRQ-UHFFFAOYSA-N 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 229930006722 beta-pinene Natural products 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- DUEPRVBVGDRKAG-UHFFFAOYSA-N carbofuran Chemical compound CNC(=O)OC1=CC=CC2=C1OC(C)(C)C2 DUEPRVBVGDRKAG-UHFFFAOYSA-N 0.000 description 1
- 239000012159 carrier gas Substances 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 229940043350 citral Drugs 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000003085 diluting agent Substances 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 235000011194 food seasoning agent Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- LCWMKIHBLJLORW-UHFFFAOYSA-N gamma-carene Natural products C1CC(=C)CC2C(C)(C)C21 LCWMKIHBLJLORW-UHFFFAOYSA-N 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- WTEVQBCEXWBHNA-JXMROGBWSA-N geranial Chemical compound CC(C)=CCC\C(C)=C\C=O WTEVQBCEXWBHNA-JXMROGBWSA-N 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 231100000869 headache Toxicity 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 208000015380 nutritional deficiency disease Diseases 0.000 description 1
- 230000010355 oscillation Effects 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000008654 plant damage Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 235000015136 pumpkin Nutrition 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 239000000341 volatile oil Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/22—Lamiaceae or Labiatae [Mint family], e.g. thyme, rosemary, skullcap, selfheal, lavender, perilla, pennyroyal, peppermint or spearmint
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P5/00—Nematocides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Dentistry (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明公开了一种吉龙草提取物在防治南方根结线虫中的应用。所述吉龙草提取物是用乙醇对吉龙草中有益物质进行提取浓缩得到的混合物。该提取物对南方根结线虫具有较高的杀灭效果，与润湿剂、分散剂、杀虫类农药混合施用可增强其作用效果，减缓南方根结线虫抗药性的产生。本发明创造性地将吉龙草提取物应用于南方根结线虫的杀灭，制备方法简单，原料成本低，制剂天然环保，抗虫效果显著，为吉龙草的高价值开发利用提供了方向，为南方根结线虫的防治提供了一种植物源环保型制剂，具有良好的开发应用前景与意义。
Description
技术领域
本发明属于农业病虫害绿色环保防治技术领域，具体涉及一种吉龙草提取物在防治南方根结线虫中的应用。
背景技术
线虫是一类低等的无脊椎动物，种类繁多，其中寄生在植物体内的称之为植物寄生线虫。植物寄生线虫是植物侵染性病害的重要病原物之一，在全世界普遍发生，为害严重。根结线虫是一类严重危害农业生产的植物寄生线虫，主要寄生在蔬菜、粮食作物、经济作物、果树、观赏植物及杂草等2000多种寄主上，热带、亚热带和温带的植物受害尤其严重，该病害发生后一般减产10％左右，严重的高达75％以上。尤其是南方根结线虫发病面积广，能侵染几乎所有栽培植物。南方根结线虫J2幼虫具有侵染植株的能力，它在土壤中感受到寄主分泌物的信号分子，并向寄主根尖部分趋向性迁移。迁移至根尖伸长区进行侵染。线虫钻入寄主根部后，利用口针将其食管腺细胞内的分泌物注射到寄主细胞内，诱导形成增大的、多核的、新陈代谢比较旺盛的并且为线虫持续提供营养物质的巨细胞。此外其细胞壁内突生长并形成典型的传递细胞，这些细胞可以增加对维管束内营养物质的吸收。根系受害后会形成大小不一性状不同的瘤状根结，开始为白色，质地柔软，后来加深成淡褐色，表面有裂纹。根系发病后其吸收和运输养分和水分的能力会下降，导致植株病弱产量降低。重病株地上部分表现更为明显，植株严重营养不良，瘦弱萎蔫，生长缓慢，叶片发黄变小，果实畸形变小或花果脱落，严重的会全株枯死。
目前，化学防治在根结线虫的防治过程中占有重要地位，在实际生产中能达到立竿见影的效果，是深受果农、菜农青睐的防治方法。但目前使用的化学杀线剂存在毒性大，污染环境，威胁人畜健康等弊端。随着公众环保意识的增强和线虫抗药性的增加，许多有效的杀线剂如神农丹、呋喃丹等已被禁用，人们更加迫切地需要安全环保的防治措施。生物防治是从生态学角度出发寻找的防治方法，以其环境相容性、绿色环保等优势成为无公害防治根结线虫的研究热点。采用生物防治的方法控制线虫，不仅能达到除害增产的效果，还可以减少化学防治带来的灾害，具有广阔的应用前景。
吉龙草，唇形科香薷属植物，也称暹罗香菜、吉笼草，一年生草本。主要生长于我国云南南部、西南部，多为人工种植，鲜见野生。《新华本草纲要》记载，吉龙草有清热、解毒、解表之功效，主要用于感冒，头痛，发热，消化不良等。由于该植物具有特殊香味，当地居民还将吉龙草当作蔬菜或佐料食用，是一种天然的植物香料。
目前对于吉龙草开发应用的相关报道较少，且多集中于提取精油的增香、抗菌方向。本发明首次公布了吉龙草提取物在杀灭南方根结线虫中的应用，为吉龙草的高价值开发利用提供了借鉴，为南方根结线虫的防治提供了一种天然植物源制剂。
发明内容
本发明的目的在于提供一种吉龙草提取物在防治南方根结线虫中的应用。其特征在于制备方法简单，原料成本低，制剂天然环保，抗虫效果显著，为吉龙草的高价值开发利用提供了方向，为南方根结线虫的防治提供了一种植物源环保型制剂。
为实现上述发明目的，本发明以阴干吉龙草为原料，经粉碎、过筛，乙醇浸提，减压浓缩等步骤，得到对南方根结线虫具有良好杀灭作用的植物提取物。具体技术方案步骤如下：
1)将阴干吉龙草进行粉碎、过筛，吉龙草粉末与70％～85％乙醇溶液以1:4～10(v/w)料液比进行混匀，置于40～50℃下，避光超声振荡40～90min，经0.22μm滤膜抽滤，滤渣重复浸提2次，合并3次抽滤滤液。
2)乙醇浸提滤液经旋转蒸发仪65℃、-0.1MPa下浓缩至原液体积的1/4，得吉龙草提取物。
取上述吉龙草提取物，以乙醇-吐温20溶液(9:1，v/v)为溶剂进行稀释，吉龙草提取物终浓度分别为62.5、125、250、500、1000μg/mL，取96孔板，向各孔中加入90μL南方根结线虫溶液(含线虫40～50条)，以16孔为一组分别加入5个浓度的吉龙草提取物溶液各10μL，以乙醇-吐温20溶液(9:1，v/v)为阴性对照，置于25℃培养箱中避光培养，24h、48h、72h时分别观察计数线虫死亡情况，计算各组致死率，公式如下：
结果表明上述吉龙草提取物在500μg/mL浓度下72h对南方根结线虫的致死率达到93.74％。
上述吉龙草提取物经GC-MS联用分析(色谱柱选用HP-5MS，以氦气为载气，流量1.0mL/min)，测定其主要组分为柠檬醛、香芹酮、β-蒎烯等。
本发明的有益效果是克服了现有技术的不足，提供一种具有杀灭南方根结线虫的以天然植物提取物及其应用。该提取物制备原料成本低，制备方法简单，所制备提取物安全环保，抗虫效果显著，其中的杀灭南方根结线虫活性成分的确定，可为揭秘防治南方根结线虫内在机理提供研究途径，为吉龙草的高价值开发利用提供了方向，为南方根结线虫的防治提供了一种植物源环保型制剂。
具体实施方式
以下结合实例对本发明的原理和特征进行描述，所举实例只用于解释本发明，并非用于限定本发明的范围。
实施例1
本发明提供一种吉龙草提取物，其制备方法如下：
1)将阴干吉龙草进行粉碎、过筛，吉龙草粉末与75％乙醇溶液以1:9(v/w)料液比进行混匀，置于45℃下，避光超声振荡90min，经0.22μm滤膜抽滤，滤渣重复浸提2次，合并3次抽滤滤液。
2)乙醇浸提滤液经旋转蒸发仪65℃、-0.1MPa下浓缩至原液体积的1/4，得吉龙草提取物。
实施例2
本发明提供一种吉龙草提取物，对其杀灭南方根结线虫进行浓度、时间梯度试验
取吉龙草提取物，以乙醇-吐温20溶液(9:1，v/v)为溶剂进行稀释，吉龙草提取物终浓度分别为62.5、125、250、500、1000μg/mL，取96孔板，向各孔中加入90μL南方根结线虫溶液(含线虫40～50条)，以16孔为一组分别加入5个浓度的吉龙草提取物溶液各10μL，以乙醇-吐温20溶液(9:1，v/v)为阴性对照，置于25℃培养箱中避光培养，24h、48h、72h时分别观察计数线虫死亡情况，计算各组致死率，公式如下：
结果表明上述吉龙草提取物在500μg/mL浓度下72h对南方根结线虫的致死率达到93.74％。
实施例3
本发明提供一种吉龙草提取物，对其杀灭不同地域、不同作物上南方根结线虫进行效果试验
2021年7月，将采集自潍坊章丘、烟台牟平高陵、烟台栖霞蛇窝泊、烟台海阳留格、烟台蓬莱村里集等地的患线虫病的西红柿、黄瓜、土豆、南瓜、茄子根系组织进行处理，通过清洗、浸泡、过筛、离心等步骤，分离得到5种作物上寄生的线虫虫体，经鉴定5种作物寄生线虫均为南方根结线虫。
取本发明吉龙草提取物，以乙醇-吐温20溶液(9:1，v/v)为溶剂进行稀释，吉龙草提取物终浓度范围在10～500μg/mL，取96孔板5个，分别向各板中加入5种作物分离到的线虫溶液90μL，再向各孔中按梯度加入各浓度吉龙草提取物稀释液，以乙醇-吐温20溶液(9:1，v/v)为阴性对照，置于25℃培养箱中避光培养，24h、48h时分别观察计数线虫死亡情况，计算各组致死率，公式如下：
将统计计算的到的24h及48h不同浓度下致死率数据进行统计分析(软件SPSS22.0)，结果显示，本发明吉龙草提取物对5种作物提取的南方根结线虫的半致死浓度(IC50)在24h时，范围在121～148μg/mL，在48h时，范围在66～101μg/mL，证明本发明吉龙草提取物对南方根结线虫具有较好的防治作用。
上述对所公开的实例的说明，仅为了使本领域专业技术人员能够实现或使用本发明。对这些实例的多种修改对本领域的专业技术人员来说将是显而易见的，且本文中所定义的一般原理可以在不脱离本发明的精神或范围的情况下，在其它实施例中实现。因此凡在本发明的精神和原则之内，所作的任何修改、等同替换、改进等，均应包含在本发明的保护范围之内。
Claims (6)
1.一种吉龙草提取物在防治南方根结线虫中的应用。
2.根据权利要求1所述一种吉龙草提取物，其制备工艺如下：吉龙草经阴干、粉碎得到吉龙草干粉，与质量分数70％～85％的乙醇溶液按料液比g:ml为1:4～10的比例充分混匀，在40～50℃下经超声仪超声提取40～90min，经0.22μm孔径的滤膜抽滤，抽滤后的滤渣重复施用乙醇溶液提取2～3次，将几次抽滤得到的所有滤液进行合并。合并后的滤液经旋转蒸发仪在65℃、-0.1MPa下浓缩至原液体积的1/4，得吉龙草提取物。
3.根据权利要求1～2所述一种吉龙草提取物在与润湿剂、分散剂混配施用以显著提高防治南方根结线虫效果中的应用，其中润湿剂添加质量为吉龙草提取物质量的0.2‰～2‰，分散剂添加质量为吉龙草提取物质量的0.1‰～15‰。
4.根据权利要求1～2所述一种吉龙草提取物在防治黄瓜、西红柿、土豆、姜、葡萄、大豆、茄子、桃中南方根结线虫的应用。
5.根据权利要求1～2所述一种吉龙草提取物在与杀虫类农药混合施用以减缓南方根结线虫产生抗药性中的应用，其中杀虫类农药质量占混合液总质量的30％～60％。
6.根据权利要求1～5所述一种吉龙草提取物在防治南方根结线虫中的施用方法如下：
1)制剂加水稀释为杀虫液，其中吉龙草提取物质量分数为1.25‰～3‰；
2)根据南方根结线虫虫害发生阶段选择不同施用量，杀虫液施用量范围是8～30kg；
3)根据不同防治需求选择施用方式为喷施或冲施；
4)根据施用土壤干湿情况选择追施0～2倍杀虫液质量的清水以增强杀虫液的作用效果；
5)根据虫害发生程度及防治需求选择追施0～2遍杀虫液，相邻两遍喷施操作间隔2～5天进行。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210941695.8A CN115191449B (zh) | 2022-08-08 | 2022-08-08 | 一种吉龙草提取物在防治南方根结线虫中的应用 |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210941695.8A CN115191449B (zh) | 2022-08-08 | 2022-08-08 | 一种吉龙草提取物在防治南方根结线虫中的应用 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115191449A true CN115191449A (zh) | 2022-10-18 |
| CN115191449B CN115191449B (zh) | 2024-01-19 |
Family
ID=83586053
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210941695.8A Active CN115191449B (zh) | 2022-08-08 | 2022-08-08 | 一种吉龙草提取物在防治南方根结线虫中的应用 |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115191449B (zh) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000053020A1 (en) * | 1999-03-10 | 2000-09-14 | Ecosmart Technologies, Inc. | Nematicidal compositions containing plant essential oils and mixtures or blends thereof |
| KR20080074809A (ko) * | 2008-04-15 | 2008-08-13 | 이동운 | 천연 식물 추출물 및 이를 포함하는 해충 방제용 조성물 |
| US20080220038A1 (en) * | 2004-01-23 | 2008-09-11 | Lanny Franklin | Nematicidal Compositions and Methods of Using Them |
| CN102676300A (zh) * | 2012-06-01 | 2012-09-19 | 云南烟草科学研究院 | 吉龙草精油及其在烟草中的应用 |
| CN104412968A (zh) * | 2013-09-06 | 2015-03-18 | 中国农业大学 | 香草醛在制备南方根结线虫杀虫抑制剂中的应用 |
| CN113180068A (zh) * | 2021-04-23 | 2021-07-30 | 云南自然谷生物开发有限公司 | 一种综合性的植物消毒液 |
-
2022
- 2022-08-08 CN CN202210941695.8A patent/CN115191449B/zh active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2000053020A1 (en) * | 1999-03-10 | 2000-09-14 | Ecosmart Technologies, Inc. | Nematicidal compositions containing plant essential oils and mixtures or blends thereof |
| US20080220038A1 (en) * | 2004-01-23 | 2008-09-11 | Lanny Franklin | Nematicidal Compositions and Methods of Using Them |
| KR20080074809A (ko) * | 2008-04-15 | 2008-08-13 | 이동운 | 천연 식물 추출물 및 이를 포함하는 해충 방제용 조성물 |
| CN102676300A (zh) * | 2012-06-01 | 2012-09-19 | 云南烟草科学研究院 | 吉龙草精油及其在烟草中的应用 |
| CN104412968A (zh) * | 2013-09-06 | 2015-03-18 | 中国农业大学 | 香草醛在制备南方根结线虫杀虫抑制剂中的应用 |
| CN113180068A (zh) * | 2021-04-23 | 2021-07-30 | 云南自然谷生物开发有限公司 | 一种综合性的植物消毒液 |
Non-Patent Citations (2)
| Title |
|---|
| 周露 等: "吉龙草精油的化学成分及抗菌活性研究", 《香料香精化妆品》, no. 8, pages 1 * |
| 李晓琼;唐军;毛润萍;黄羽;郭文锋;: "圆叶葡萄叶提取物对南方根结线虫的生物活性", 南方农业学报, no. 10, pages 74 - 78 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115191449B (zh) | 2024-01-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Patel | A weed with multiple utility: Lantana camara | |
| Raghavendra et al. | Laboratory studies on mosquito larvicidal efficacy of aqueous & hexane extracts of dried fruit of Solanum nigrum Linn | |
| Shafique et al. | Role of antagonistic microorganisms and organic amendment in stimulating the defense system of okra against root rotting fungi | |
| Adekunle et al. | Nematicidal effects of Leucaena leucocephala and Gliricidia sepium extracts on Meloidogyne incognita infecting okra | |
| Kumar et al. | Potential of Pongamia glabra vent as an insecticide of plant origin | |
| Meyer et al. | Evaluation of roselle (Hibiscus sabdariffa) leaf and pomegranate (Punica granatum) fruit rind for activity against Meloidogyne incognita | |
| Kishore et al. | Control of late leaf spot of groundnut (Arachis hypogaea) by extracts from non-host plant species | |
| Varun et al. | Larvicidal activity of leaf extract of some weeds against malaria vector Anopheles stephensi | |
| Abo-Elyousr et al. | Management of tomato root-knot nematode Meloidogyne incognita by plant extracts and essential oils | |
| CN103880563A (zh) | 一种栽培基质及其制备方法与应用 | |
| Vinodhini et al. | Effect of plant extracts on root-knot nematode meloidogyne incognita infecting tomato | |
| Murslain et al. | Efficacy of Moringa leaves and Trichoderma harzianum on the invasion and development of Meloidogyne javanica | |
| CN115191449A (zh) | 一种吉龙草提取物在防治南方根结线虫中的应用 | |
| CN103222469B (zh) | 一种使用酶解法生产苦参碱水剂的方法 | |
| CN1439270A (zh) | 对线虫卵高致病率的淡紫拟青霉菌剂的制备方法 | |
| CN113349203A (zh) | 苯甲酸在防治植物线虫病害中的应用 | |
| Elrehawy et al. | The Insecticidal and Antifeedant Activity of Calotropis procera Latex and Foliar Extracts Against the Red Palm Weevil, Rhynchophorus ferrugineus (Olivier)(Coleoptera: Curculionidae) | |
| Kumar et al. | Host specificity of Gibbago trianthemae Simmons, a Phaeodictyoconidial Hyphomycetes fungus on Trianthema portulacastrum L.(Horse purslane) | |
| CN111184032B (zh) | 一种景洪哥纳香杀线虫提取物及其制备方法和应用 | |
| CN111567571B (zh) | 一种植物源复配用于南方根结线虫的杀虫剂及其制备方法 | |
| CN111869683B (zh) | 一种提高油萝卜抗根结线虫效率的方法 | |
| Mamman | Nematicidal Activity of Ageratum conyzoides Leaf Extract Against Root-knot Nematode (Meloidogyne javanica) on Eggplant in Jalingo, Nigeria | |
| Lakmini et al. | Nemato-toxic potential of Trichoderma harzianum and T. viride extra-cellular metabolites and Annona glabra crude extraction on Meloidogyne incognita infestation | |
| Samaliev et al. | Management of root-knot nematode Meloidogyne hapla on strawberry plant with some plant extracts | |
| MEENA et al. | Biocontrol Efficacy of Thevetia Peruviana Bioformulations Against Early Blight Disease of Potato |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |