CN115184495A - Corn stalk sugar content measuring device - Google Patents
Corn stalk sugar content measuring device Download PDFInfo
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- CN115184495A CN115184495A CN202210804494.3A CN202210804494A CN115184495A CN 115184495 A CN115184495 A CN 115184495A CN 202210804494 A CN202210804494 A CN 202210804494A CN 115184495 A CN115184495 A CN 115184495A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
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- General Health & Medical Sciences (AREA)
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Abstract
The invention relates to the technical field of sugar content detection, in particular to a corn stalk sugar content measuring device with both accuracy and high efficiency, which comprises a first sample box and a second sample box, and is characterized in that: the first sample box is used for containing filtered sugar extracting solution, and the second sample box is used for containing a corn stalk sample with a plurality of sections; the liquid chromatograph is matched with the first sample box for use, comprises an RID-10A refractive index detector, and calculates the content of the sugar according to the peak area of the sample and the standard curve of each sugar. By adopting the scheme of the invention, the sugar content of the corn stalks can be detected by adopting a hue analysis method, so that the detection accuracy is improved; under the condition of large sample amount, a section detection method can be adopted to improve the detection efficiency of the corn stalks, and a hue analysis method is adopted to detect the sugar content of the corn stalks, so that the method can be used for result verification of the section detection method, and the accuracy and the high efficiency are both considered.
Description
Technical Field
The invention relates to the technical field of sugar detection, in particular to a corn stalk sugar determination device.
Background
The silage corn is a special corn which is obtained from overground part plants including fruit clusters in the late stage of corn milk maturity to the stage of wax maturity, is prepared into silage through processes of chopping, processing and storing, fermenting and the like, and is used for feeding herbivorous livestock mainly including cattle and sheep, is an important coarse feed source for producing non-staple products such as milk, meat and the like in the world, is an important support for developing the animal husbandry in China, and has important guiding significance for adjusting the current planting industry structure in China. At present, the international silage corn plays an important role in modern agriculture and animal husbandry due to the unique advantages of the silage corn. In developed areas of agriculture and animal husbandry such as Europe and America, particularly in developed areas of dairy cows, beef cattle, milch goats and mutton sheep, silage corns are planted on farms in a large quantity to serve as high-quality feed; the utilization of the whole silage corn is very popular in developed countries of animal husbandry such as Europe and America, and the silage corn is greatly supported and popularized by the countries, the application range is very wide, and the silage corn almost completely covers herbivorous livestock; the area of planted silage corns in the United states is kept between 3500 and 4500 ten thousand mu in recent years, which accounts for about 10 percent of the total planting area of the corns, and the area of planted silage corns in Germany is kept between 3000 and 3500 ten thousand mu, which accounts for about 90 percent of the total planting area of the corns. The silage corns meet the rapid development period under the support of the structure adjustment of the planting industry and the policy of changing grains into feeds in China, have larger development space, and have increasingly large requirements on animal products and dairy products along with the continuous improvement of the living standard of people, the use of the high-quality silage corns as the coarse feed is the simplest method for improving the milk yield of dairy cows and improving the milk quality, in order to meet the requirements of the animal husbandry on the high-quality coarse feed, the cultivation and the large-scale planting of the high-quality silage corns are very important, the planting area of the whole high-quality silage corns in 2018 years reaches more than 2000 ten thousand mu, and the silage corn area reaches 3500 ten thousand mu in 2020.
After the corn stalks are subjected to anaerobic fermentation, the pH value of the corn stalks is reduced to 4.0The method can inhibit growth of harmful bacteria, and can be used for preparing silage for long-term storage. Sugar is a raw material for lactic acid bacteria to form lactic acid in the anaerobic fermentation process, and the lactic acid bacteria can produce enough lactic acid under the condition of enough sugar content [1-4] Low lactic acid concentrations lead to a reduction in silage quality and to a greater tendency for spoilage. Researches indicate that the stalk high-sugar corn can also improve the palatability of silage and increase the feed intake of livestock. That is, the high sugar content in the corn stalk is an important condition for increasing the fermentation quality of the silage corn feed and improving the utilization rate and the palatability of the silage corn, so that the silage corn with high sugar quality resources is preferably selected by detecting the sugar content of the corn stalk, and the necessity is very high.
However, the existing device for measuring the sugar content of the corn stalks cannot always give consideration to both accuracy and high efficiency.
Disclosure of Invention
The invention aims to provide a corn stalk sugar content measuring device which has both accuracy and high efficiency.
Maize stalk sugar surveys device, including first sample case and second sample case, its characterized in that: the first sample box is used for containing the filtered sugar extracting solution, and the second sample box is used for containing a corn stalk sample with a plurality of sections; the liquid chromatograph is matched with the first sample box for use, comprises an RID-10A refractive index detector, and calculates the content of the sugar according to the peak area of the sample and the standard curve of each sugar.
Has the beneficial effects that: by adopting the scheme of the invention, the sugar content of the corn stalks can be detected by adopting a hue analysis method, so that the determination accuracy is improved; under the condition of large sample amount, a section detection method can be adopted to improve the detection efficiency of the corn stalks, and a hue analysis method is adopted to detect the sugar content of the corn stalks, so that the method can be used for verifying the result of two rows of sugar content detection of the corn stalks by the section detection method, and the accuracy and the high efficiency are both considered.
Further, the sugar extract is: taking ear position nodes, the upper node of the ear position and the lower node of the ear position, removing female ears, leaves and leaf sheaths in 3 internodes, adding liquid nitrogen, quickly freezing, adding liquid nitrogen into the mixture, grinding into powder, taking 0.1-0.2g, adding 2mL of 80% ethanol, mixing uniformly, leaching at 80 ℃ for 2h, centrifuging at 12000r/min for 10min, taking 1mL of supernatant, concentrating, drying, dissolving the precipitate with 1mL of ultrapure water, and filtering to obtain the compound.
Has the beneficial effects that: the sugar extracting solution is simple to prepare, low in cost, and capable of ensuring that the sugar does not run off when the extracting solution is prepared and ensuring the accuracy of detection.
Furthermore, the side door has been seted up to the side at the second sample case, be equipped with three clamping piece that is used for centre gripping maize stem stalk sample in the second sample case, every clamping piece all has the clamping part of indent, middle clamping piece and both sides clamping piece dislocation set, one side that every clamping piece dorsad clamping part all is provided with the action bars that can drive the clamping piece and throw off maize stem stalk sample, the action bars orientation of one of them clamping piece among the action bars of middle clamping piece and the clamping piece of both sides is unanimous, wherein the action bars of middle clamping piece can drive middle clamping piece 180 rotations in the vertical plane.
The method comprises the steps of establishing a model of the sugar content of the corn stalks and the near infrared spectrum on the sections of the corn stalks, and measuring the sugar content of the corn stalks to be detected by using the model. In the prior art, the method for detecting the sugar content of the corn stalks by adopting the near infrared spectrum has the following defects: only one section can be scanned each time, the scanning needs to be stopped after one section is scanned, the sample is taken out and is scanned after one section is replaced, the operation is complex, and the scanning efficiency is low.
The principle of the scheme is as follows: the method for detecting the sugar content of the corn stalk by adopting the near infrared spectrum needs to scan seven sections of a corn stalk sample, namely two new sections obtained by transversely cutting two ends of the corn stalk sample, transversely cutting different parts of the corn stalk sample twice and longitudinally cutting three sections of the corn stalk sample. Therefore, the two ends of the corn stalk are cut off to meet the length requirement of the corn stalk, a corn stalk sample is formed, meanwhile, the cross sections of the two ends of the corn stalk sample are cut off, then, the cross sections are cut twice to obtain two new cross sections, and finally, the corn stalk samples of the three sections are longitudinally cut off, and the longitudinal sections of the three sections are cut off.
The corn stalk samples of each section are arranged in a sample box in a longitudinal cutting mode, then the corn stalk samples of each section are respectively placed into the sample box through a side door, wherein the corn stalk samples of the middle section are clamped in a clamping part of a middle clamping piece, one end of the corn stalk samples of the middle section faces upwards (two end faces are new transverse sections), the corn stalk samples of the two side sections are clamped in the clamping parts of the clamping pieces of the two sides, the transverse sections of the two ends of the corn stalk samples of the two side sections face upwards, the transverse sections of the two ends and one of the new transverse sections are scanned, after the scanning, the middle clamping piece is driven by an operating rod to rotate 180 degrees in a vertical plane, so that the other new transverse section faces upwards, the other new transverse section is scanned again, after the scanning, the three clamping pieces are respectively driven by the three operating rods, the corn stalk samples of the three sections are clamped without the clamping pieces, the longitudinal sections which are originally arranged together are gradually separated, namely, the corn stalk samples of each section are scattered, the longitudinal sections face upwards, and the whole sample scanning of the three longitudinal sections can be completed.
The beneficial effect of this scheme lies in: in the scanning process of seven sections of the corn stalk sample, the corn stalk sample in three sections is placed into the second sample box only once, the operating rod of the middle clamping piece is operated once in the process, and then the three operating rods are respectively operated to drive the three clamping pieces to separate from the corn stalk sample in each section, so that the whole scanning of the seven sections of the whole corn stalk sample can be completed.
Furthermore, the end part of each operating rod extends out of a second sample box, a rotating nut in threaded connection with the end part of the operating rod is arranged at the position, located at the end part of each operating rod, of the second sample box, the inner end face of each rotating nut abuts against the outer wall of the second sample box, and an anti-rotating structure is arranged between each second sample box and each operating rod.
Has the advantages that: and an anti-rotation structure is arranged between the second sample box and each operating rod, so that when the rotating nut rotates, the operating rod cannot be driven to rotate, and the operating rod only can do linear motion, so that the clamping piece is driven to be separated from the corn stalk sample.
Furthermore, the anti-rotation structure include the location strip of setting on the second sample case outer wall, it has the guide way unanimous with the action bars direction of motion to open on the location strip, is equipped with the gib block at the tip of action bars, the gib block can slide in the guide way.
Has the beneficial effects that: through the setting of location strip and gib block, realize the direction of action bars when the motion to avoid the action bars to rotate along with swivel nut together.
Furthermore, the positioning strip matched with the middle operating rod is an elastic positioning strip.
Has the advantages that: the positioning strip matched with the middle operating rod is designed into the elastic positioning strip, so that when the positioning strip is required to be guided, the guide strip is stretched into the guide groove in the positioning strip, and when the guide strip is not required, the operating rod is required to rotate along with the rotating nut, so that when the middle clamping piece is driven to rotate by 180 degrees, the guide strip is withdrawn from the guide groove in the positioning strip, and the limitation on the operating rod is relieved.
The corn harvester further comprises three groups of linkage ejection structures with the same structure, each group of linkage ejection structures comprises a first ejector rod arranged on the side face of the operating rod and facing upwards, the end face of the first ejector rod is provided with a first inclined plane, the inner wall of the sample box is provided with a vertical guide groove, a second ejector rod capable of sliding up and down along the vertical guide groove is arranged in the vertical guide groove, the lower end of the second ejector rod is provided with a second inclined plane capable of offsetting against the first inclined plane, the top of the second sample box is provided with a horizontal guide hole, the horizontal guide hole is communicated with the guide groove, the upper end of the second ejector rod extends into the horizontal guide hole, the upper end of the second ejector rod is provided with a third inclined plane, a third ejector rod capable of sliding along the horizontal guide hole is arranged in the horizontal guide hole, the right end of the third ejector rod is provided with a fourth inclined plane capable of offsetting against the third inclined plane, the left end of the third ejector rod is provided with a fifth inclined plane, the top of the second ejector rod is provided with a vertical guide hole, the vertical guide hole is communicated with the horizontal guide hole, the fourth ejector rod penetrates through the vertical guide hole, the fifth inclined plane is provided with a fifth ejector rod capable of offsetting against the fifth inclined plane, and the lower end of extending into the fourth ejector rod, and the corn stalk longitudinal push rod.
The principle of the design scheme is as follows:
when the operating rod stretches out to the outside of the second sample box, the first inclined plane of the first ejector rod supports the second inclined plane of the second ejector rod to force the second ejector rod to move upwards, the third inclined plane at the upper end of the second ejector rod supports the fourth inclined plane of the third ejector rod gradually to force the third ejector rod to move leftwards, so that the fifth inclined plane of the third ejector rod supports the sixth inclined plane at the upper end of the fourth ejector rod to force the fourth ejector rod to move downwards, the blade part of the fourth ejector rod is in contact with two longitudinal sections of the corn stalk sample in each section and force the two longitudinal sections to be separated, and therefore the corn stalk sample is prevented from toppling randomly when the clamping piece is disengaged from the corn stalk sample.
The beneficial effect of this design lies in:
the corn stalk sample processing device has the advantages that extra power is not required to be added, the separation of two longitudinal sections of the corn stalk sample is realized simultaneously in the process that the operating rod extends outwards, and the scanning of the longitudinal sections of the corn stalk sample in each section is smoother.
Furthermore, a return spring is arranged between the fourth ejector rod and the top of the second sample box.
Has the advantages that: through setting up reset spring, can realize the automatic re-setting of fourth ejector pin, do not influence the action next time, and play the effect of taut fourth ejector pin.
Furthermore, the bottom of the second sample box is provided with three placing grooves in parallel, the three placing grooves are respectively positioned under the three clamping pieces, and the orientation of the three placing grooves is perpendicular to the opening direction of the clamping part of each clamping piece.
Has the beneficial effects that: the setting of standing groove, the effect has two, one is the in-process that disperses at the maize stalk sample of three district section, play the effect of direction to the maize stalk sample of every district section, make the maize stalk sample of every district section can not disturb each other at the in-process that disperses, and only can disperse along the direction of guide way, another is exactly the in-process that breaks away from the maize stalk sample at the clamping piece, play the effect of location to the maize stalk sample, make the maize stalk sample can not follow the clamping piece easily and empty, can make the clamping piece throw off with the maize stalk sample smoothly.
Further, the liquid chromatograph of the corn stalk sugar content measuring device adopts a Class-vp data processing system.
Drawings
FIG. 1 is a schematic view showing the structure of an embodiment of a corn stalk sugar measuring apparatus according to the present invention.
FIG. 2 is a schematic view of a linkage ejection structure in an embodiment of the corn stalk sugar content measuring apparatus of the present invention.
Detailed Description
The following is further detailed by way of specific embodiments:
reference numerals in the drawings of the specification include: second sample box 1, clamping piece 2, standing groove 3, action bars 4, swivel nut 5, gib block 6, location strip 7, first ejector pin 8, first inclined plane 9, second ejector pin 10, second inclined plane 11, third inclined plane 12, third ejector pin 13, fourth inclined plane 14, fifth inclined plane 15, fourth ejector pin 16, sixth inclined plane 17, cutting part 18, reset spring 19, maize stem stalk sample 20, side door 21, vertical guide way 22, horizontal guiding hole 23, vertical guiding hole 24.
The corn stalk sugar content measuring device disclosed by the embodiment comprises a first sample box and a second sample box.
The first sample box is used for containing filtered sugar extracting solution, and the sugar extracting solution is as follows: taking ear position nodes, one node above the ear position and one node below the ear position, removing female ears, leaves and leaf sheaths, adding liquid nitrogen for quick freezing, storing at-80 ℃ for sugar content determination, after quick freezing, taking a mixture, adding liquid nitrogen, grinding into powder, taking 0.1-0.2g, adding 2mL of 80% ethanol, mixing uniformly, leaching at 80 ℃ for 2h, centrifuging at 12000r/min for 10min, taking 1mL of supernatant, concentrating and drying, dissolving precipitates with 1mL of ultrapure water, and filtering to obtain the product.
The corn stalk sugar content measuring device in the embodiment further comprises a liquid chromatograph matched with the first sample box, wherein the liquid chromatograph comprises an RID-10A differential refraction detector, and the liquid chromatograph adopts a Class-vp data processing system. Working conditions of a chromatographic column of a liquid chromatograph: the column temperature was 40 ℃ and the flow rate was 1.0mL/min, and the volume of each injection was 20. Mu.L. And the liquid chromatograph calculates the content of the sugar according to the peak area of the sample and the standard curve of each sugar, and repeats for 3 times, wherein each repetition is ensured to be an independent sample. The total sugar content is calculated as the sum of the fructose, glucose and sucrose contents.
In this embodiment, the second sample box is used for containing a corn stalk sample with a plurality of cut surfaces; as shown in figures 1 and 2: the corn stalk sugar content measuring device disclosed by the embodiment is provided with a Pbs detector and OPUS analysis software, the OPUS analysis software is used for carrying out mathematical processing on absorption spectrum data, near infrared spectrum is adopted for measuring the sugar content of a corn stalk sample 20, the method is to establish a model of the corn stalk sugar content and the near infrared spectrum on a corn stalk section, the sugar content of the corn stalk to be detected is measured by applying the model, and the modeling method is as follows: actually measuring the actual sugar content of a plurality of samples by a chemical method (such as a phenol method, an anthrone colorimetric method and the like), scanning the near infrared spectrum of the sections of the plurality of samples, and establishing the corresponding relation of the actual sugar content and the near infrared spectrum of the sections of the plurality of samples. In the modeling process, abnormal samples are also removed, a sample set and a test set are established for the remaining sample set from which the abnormal samples are removed, the near infrared spectrum is preprocessed and screened for characteristic wavelengths, and a partial least square method is adopted to establish a near infrared spectrum model of the sugar content of the corn stalks. The above method is the prior art and is not described herein again.
In the method, whether in the process of establishing a model or in the process of applying the model, seven sections of the corn stalks need to be scanned one by near infrared spectrums, wherein the seven sections are the cross sections at two ends of the corn stalk sample 20, two new cross sections obtained by transversely cutting different parts of the corn stalk sample 20 twice and the longitudinal sections of three sections of the corn stalk sample 20.
In the corn stalk sugar measuring device of this embodiment, different from the prior art that adopts the rotatory sample cup, a second sample case 1 is designed to this embodiment scheme, has seted up side door 21 in the side of second sample case 1, and side door 21 is the side door 21 that has the leak protection light function, for example side door 21 gilds.
Be equipped with three clamping piece 2 that are used for centre gripping maize stalk sample 20 in second sample case 1 the bottom parallel arrangement of second sample case 1 has three standing groove 3, and three standing groove 3 is equallyd divide and is located three clamping piece 2 under, and the orientation of three standing groove 3 all is perpendicular to the opening direction of the clamping part of every clamping piece 2. Every clamping piece 2 all has the clamping part to the indent, and the size design of clamping part just can contact with maize stalk periphery, and can not be too tight to maize stalk sample 20 centre gripping, designs into sheet structure simultaneously, all is that convenient and maize stalk sample 20's surface is thrown off, and can not lead to can't throwing off or can drive maize stalk sample 20 and empty because of centre gripping tension or centre gripping dynamics are too big.
In this embodiment, middle clamping piece 2 and the dislocation set of both sides clamping piece 2, one side of every clamping piece 2 back to the clamping part all is provided with can drive clamping piece 2 and throw off the action bars 4 of maize stalk sample 20, the action bars 4 of middle clamping piece 2 is unanimous with the action bars 4 orientation of one of them clamping piece 2 in the clamping piece 2 of both sides, the tip of every action bars 4 all stretches out second sample case 1, the position that lies in 4 tip of every action bars at second sample case 1 all is equipped with the swivel nut 5 with 4 tip threaded connection of action bars, the interior terminal surface of swivel nut 5 offsets with 1 outer wall of second sample case, all be equipped with between second sample case 1 and every action bars 4 and prevent rotating-structure.
In this embodiment, the anti-rotation structure includes a positioning strip 7 disposed on the outer wall of the second sample box 1, wherein the positioning strip 7 engaged with the middle operating rod 4 is an elastic positioning strip 7. The positioning strip 7 is provided with a guide groove consistent with the movement direction of the operating rod 4, the end part of the operating rod 4 is provided with a guide strip 6, and the guide strip 6 can slide in the guide groove. The middle operating rod 4 is matched with the positioning strip 7, the positioning strip 7 is elastic, when the middle operating rod 4 is not required to be guided, namely the middle operating rod 4 is required to rotate along with the rotary nut 5, when the operating rod 4 drives the middle clamping piece 2 to rotate 180 degrees, the guide strip 6 is withdrawn from the guide groove on the positioning strip 7, and the limitation on the operating rod 4 is removed.
In this embodiment, still including the linkage that three group's structures are the same and push up the structure, every linkage pushes up and opens the structure and all includes the first ejector pin 8 that sets up face up at action bars 4 sides, the terminal surface of first ejector pin 8 has first inclined plane 9, it has vertical guide way 22 to open at the inner wall of second sample case 1, be equipped with in the vertical guide way 22 and follow vertical guide way 22 gliding second ejector pin 10 from top to bottom, the lower extreme of second ejector pin 10 has the second inclined plane 11 that can offset with first inclined plane 9, when upwards supporting second ejector pin 10 lower extreme second inclined plane 11 at first inclined plane 9 of first ejector pin 8, can force second ejector pin 10 upwards to move along vertical guide way 22.
The top of the second sample box 1 is provided with a horizontal guide hole 23, the horizontal guide hole 23 is communicated with the guide groove, the upper end of the second ejector rod 10 extends into the horizontal guide hole 23, the upper end of the second ejector rod 10 is provided with a third inclined surface 12, a third ejector rod 13 capable of sliding along the horizontal guide hole 23 is arranged in the horizontal guide hole 23, the right end of the third ejector rod 13 is provided with a fourth inclined surface 14 capable of abutting against the third inclined surface 12, and after the third inclined surface 12 of the second ejector rod 10 abuts against the fourth inclined surface 14 of the third ejector rod 13, the second ejector rod 10 can force the third ejector rod 13 to move leftwards along the horizontal guide hole 23.
The left end of the third top rod 13 is provided with a fifth inclined plane 15, the top of the second sample box 1 is provided with a vertical guide hole 24, the vertical guide hole 24 is communicated with a horizontal guide hole 23, a vertical fourth top rod 16 penetrates through the vertical guide hole 24, the upper end of the fourth top rod 16 is provided with a sixth inclined plane 17 which can be abutted against the fifth inclined plane 15 so as to force the fourth top rod 16 to slide downwards, and the lower end of the fourth top rod 16 is provided with a blade part 18 which can extend into the longitudinal section of the corn stalk. When the fourth push rod 16 moves downwards along the vertical guide hole 24, the two longitudinal sections of the corn stalk sample 20 of one section which is originally aligned together can be separated by the blade part 18 arranged at the lower end of the fourth push rod 16, so that the corn stalk sample 20 of one section is smoothly dispersed without being constrained by the clamping piece 2, the longitudinal sections face upwards, and the scanning is convenient.
And a return spring 19 is arranged between the fourth ejector rod 16 and the top of the second sample box 1, and the return spring 19 is a tension spring. The concrete structure is that a horizontal installation strip is arranged on the periphery of the fourth ejector rod 16, and a tension spring is installed between the horizontal installation strip and the inner wall of the top of the second sample box 1.
While the foregoing is directed to embodiments of the present invention, other and further embodiments of the invention may be devised without departing from the basic scope thereof, and the scope thereof is determined by the claims that follow. It should be noted that, for those skilled in the art, without departing from the structure of the present invention, several changes and modifications can be made, which should also be regarded as the protection scope of the present invention, and these will not affect the effect of the implementation of the present invention and the practicability of the patent. The scope of the claims of the present application shall be determined by the contents of the claims, and the description of the embodiments and the like in the specification shall be used to explain the contents of the claims.
Claims (10)
1. Maize stalk sugar surveys device, including first sample case and second sample case, its characterized in that: the first sample box is used for containing the filtered sugar extracting solution, and the second sample box is used for containing a corn stalk sample with a plurality of sections; the liquid chromatograph is matched with the first sample box, comprises an RID-10A refractive index detector, and calculates the content of the sugar according to the sample peak area and the standard curve of each sugar.
2. The corn stalk sugar content measuring apparatus according to claim 1, wherein: the sugar extracting solution comprises: taking ear position nodes, the upper node of the ear position and the lower node of the ear position, removing female ears, leaves and leaf sheaths in 3 internodes, adding liquid nitrogen, quickly freezing, adding liquid nitrogen into the mixture, grinding into powder, taking 0.1-0.2g, adding 2mL of 80% ethanol, mixing uniformly, leaching at 80 ℃ for 2h, centrifuging at 12000r/min for 10min, taking 1mL of supernatant, concentrating, drying, dissolving the precipitate with 1mL of ultrapure water, and filtering to obtain the compound.
3. The corn stalk sugar measuring device according to claim 1, characterized in that: the side door has been seted up to the side at the second sample box, be equipped with the three clamping piece that is used for centre gripping maize stalk sample in the second sample box, every clamping piece all has the clamping part to the indent, middle clamping piece and both sides clamping piece dislocation set, one side of every clamping piece clamping part dorsad all is provided with the action bars that can drive the clamping piece and throw off maize stalk sample, the action bars orientation of one of them clamping piece in the action bars of middle clamping piece and the clamping piece of both sides is unanimous, wherein the action bars of middle clamping piece can drive middle clamping piece 180 rotations in the vertical plane.
4. The corn stalk sugar measuring device according to claim 3, characterized in that: the end part of each operating rod extends out of the second sample box, a rotating nut in threaded connection with the end part of the operating rod is arranged at the position, located at the end part of each operating rod, of the second sample box, the inner end face of the rotating nut abuts against the outer wall of the second sample box, and an anti-rotating structure is arranged between the second sample box and each operating rod.
5. The corn stalk sugar content measuring apparatus according to claim 4, wherein: the anti-rotation structure comprises a positioning strip arranged on the outer wall of the second sample box, a guide groove consistent with the motion direction of the operating rod is formed in the positioning strip, the guide strip is arranged at the end part of the operating rod, and the guide strip can slide in the guide groove.
6. The corn stalk sugar measuring device according to claim 5, characterized in that: the positioning strip matched with the middle operating rod is an elastic positioning strip.
7. The corn stalk sugar measuring device according to claim 3 or 5, characterized in that: the corn harvester is characterized by further comprising three groups of linkage ejection structures with the same structure, each group of linkage ejection structures comprises a first ejector rod arranged on the side face of the operating rod and facing upwards, the end face of the first ejector rod is provided with a first inclined plane, the inner wall of the sample box is provided with a vertical guide groove, a second ejector rod capable of sliding up and down along the vertical guide groove is arranged in the vertical guide groove, the lower end of the second ejector rod is provided with a second inclined plane capable of offsetting against the first inclined plane, the top of the second sample box is provided with a horizontal guide hole, the horizontal guide hole is communicated with the guide groove, the upper end of the second ejector rod extends into the horizontal guide hole, the upper end of the second ejector rod is provided with a third inclined plane, a third ejector rod capable of sliding along the horizontal guide hole is arranged in the horizontal guide hole, the right end of the third ejector rod is provided with a fourth inclined plane capable of offsetting against the third inclined plane, the left end of the third ejector rod is provided with a fifth inclined plane, the top of the second sample box is provided with a vertical guide hole, the vertical guide hole is communicated with the horizontal guide hole, the fourth ejector rod penetrates through the vertical guide hole, the upper end of the fourth ejector rod capable of offsetting against the fifth inclined plane, and the lower end of extending into the fourth ejector rod, and the corn stalk sliding downwards.
8. The corn stalk sugar measuring device according to claim 7, characterized in that: and a return spring is arranged between the fourth ejector rod and the top of the second sample box.
9. The corn stalk sugar measuring device according to claim 3, characterized in that: the bottom of the second sample box is provided with three placing grooves in parallel, the three placing grooves are respectively positioned under the three clamping pieces, and the orientation of the three placing grooves is perpendicular to the opening direction of the clamping part of each clamping piece.
10. The corn stalk sugar measuring device according to claim 1, characterized in that: the liquid chromatograph adopts a Class-vp data processing system.
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