CN115184495B - Corn stalk sugar determination device - Google Patents
Corn stalk sugar determination device Download PDFInfo
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- CN115184495B CN115184495B CN202210804494.3A CN202210804494A CN115184495B CN 115184495 B CN115184495 B CN 115184495B CN 202210804494 A CN202210804494 A CN 202210804494A CN 115184495 B CN115184495 B CN 115184495B
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/3563—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light for analysing solids; Preparation of samples therefor
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/25—Colour; Spectral properties, i.e. comparison of effect of material on the light at two or more different wavelengths or wavelength bands
- G01N21/31—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry
- G01N21/35—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light
- G01N21/359—Investigating relative effect of material at wavelengths characteristic of specific elements or molecules, e.g. atomic absorption spectrometry using infrared light using near infrared light
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/04—Preparation or injection of sample to be analysed
- G01N30/06—Preparation
- G01N30/14—Preparation by elimination of some components
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/02—Column chromatography
- G01N30/62—Detectors specially adapted therefor
- G01N30/74—Optical detectors
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- General Health & Medical Sciences (AREA)
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Abstract
The application relates to the technical field of sugar detection, in particular to a corn stalk sugar determination device with both accuracy and high efficiency, which comprises a first sample box and a second sample box, and is characterized in that: the first sample box is used for containing filtered sugar extract, and the second sample box is used for containing corn stalk samples with a plurality of sections; the liquid chromatograph is matched with the first sample box for use, the liquid chromatograph comprises a RID-10A differential refraction detector, and the liquid chromatograph calculates the content of sugar according to the peak area of the sample and the standard curve of each sugar. By adopting the scheme of the application, the corn stalk sugar content can be detected by adopting a hue analysis method, so that the determination accuracy is improved; under the condition of large sample quantity, the detection efficiency of the corn stalks can be improved by adopting a section detection method, and the sugar content of the corn stalks can be detected by adopting a hue analysis method, so that the method can be used for verifying the result of the section detection method, thereby taking accuracy and high efficiency into account.
Description
Technical Field
The application relates to the technical field of sugar detection, in particular to a corn stalk sugar determination device.
Background
Silage corn refers to silage prepared by harvesting overground plants including clusters from the late stage of corn milk ripening to the stage of wax ripening through the processes of chopping, processing, storage, fermentation and the like, is a special corn for feeding herbivorous domestic animals mainly cattle and sheep, is an important source of coarse fodder for producing milk, meat and other subsidiary food products in the world, is an important support for developing animal husbandry in China, and has important guiding significance for adjusting the current planting industry structure in China. At present, the international silage corns play an important role in the modern agriculture and animal husbandry by virtue of the special advantages thereof. In the areas developed in agriculture and animal husbandry such as Europe and America, especially in the areas developed in production of cows, beef cattle, milch goats and mutton sheep, silage corns are planted in large quantities on farms as high-quality feeds; the utilization of whole silage corns is very popular in developed countries of European and American animal husbandry and is greatly supported and popularized by the countries, the application level is very wide, and almost complete
Fully covering herbivorous livestock; in recent years, the area of the silage corns planted in the United states is kept between 3500 and 4500 mu, which accounts for about 10% of the total planting area of corns, and the area of the silage corns planted in Germany is kept between 3000 and 3500 mu, which accounts for about 90% of the total planting area of corns. The silage corns meet the support of the structure adjustment and the grain improvement feeding policy of the planting industry in China, have a large development space, and with the continuous improvement of the living standard of people, the requirements on animal products and dairy products are increasingly increased, the use of high-quality silage corns as roughage is the simplest method for improving the milk yield of dairy cows and improving the milk quality, in order to meet the requirements of the animal industry on the high-quality roughage, the cultivation and mass planting of the high-quality silage corns are very important, the planting area of the whole plant of the high-quality silage corns in 2018 reaches more than 2000 ten thousand mu, and the silage corn area reaches 3500 ten thousand mu by the year 2020.
The PH value of the cornstalk is reduced to below 4.0 after anaerobic fermentation, and the cornstalk can inhibit the growth of harmful fungi and can be made into silage stored for a long time. Sugar is a raw material for lactic acid formation by lactic acid bacteria during anaerobic fermentation, and the lactic acid bacteria can only produce enough lactic acid under the condition of enough sugar, and the low concentration of lactic acid can lead to the quality degradation and the easier deterioration of silage. Researches indicate that the stalk high sugar corn can also improve the palatability of silage and increase the feed intake of livestock. That is, the high sugar content in the corn stalks is an important condition for increasing the fermentation quality of the silage and improving the utilization rate and palatability of the silage, so that silage corns with high sugar germplasm resources are preferred by detecting the sugar content of the corn stalks, which is necessary.
However, the conventional device for measuring the sugar content of the cornstalk often cannot achieve both accuracy and high efficiency.
Disclosure of Invention
The application aims to provide a corn stalk sugar determination device which has both accuracy and high efficiency.
Corn stalk sugar determination device, including first sample box and second sample box, its characterized in that: the first sample box is used for containing filtered sugar extract, and the second sample box is used for containing corn stalk samples with a plurality of sections; the liquid chromatograph is matched with the first sample box for use, the liquid chromatograph comprises a RID-10A differential refraction detector, and the liquid chromatograph calculates the content of sugar according to the peak area of the sample and the standard curve of each sugar.
The beneficial effects are that: by adopting the scheme of the application, the corn stalk sugar content can be detected by adopting a hue analysis method, so that the determination accuracy is improved; under the condition of large sample quantity, the detection efficiency of the corn stalks can be improved by adopting a section detection method, the sugar content of the corn stalks can be detected by adopting a hue analysis method, and the method can be used for verifying the result of detecting the sugar content of the corn stalks by adopting the section detection method, so that the accuracy and the high efficiency are both considered.
Further, the sugar extract is: removing female ear, leaf and leaf sheath from 3 segments of ear segment, ear upper segment and ear lower segment, adding liquid nitrogen, quick freezing, adding liquid nitrogen into the mixture, grinding into powder, adding 0.1-0.2. 0.2 g, adding 80% ethanol 2 mL, mixing, leaching at 80deg.C for 2 hr, centrifuging at 12 r/min for 10 min, concentrating and drying 1 mL supernatant, dissolving precipitate with 1 mL ultrapure water, and filtering.
The beneficial effects are that: the sugar extracting solution is simple to manufacture and low in cost, and can ensure that sugar cannot be lost when the extracting solution is manufactured, and ensure the detection accuracy.
Further, the side door is offered at the side of second sample box, be equipped with three clamping pieces that are used for centre gripping corn stalk sample in the second sample box, every clamping piece all has the clamping part of indent, middle clamping piece and both sides clamping piece dislocation set, every clamping piece all is provided with the action bars that can drive the clamping piece and disengage corn stalk sample in the side of clamping part dorsad, the action bars of middle clamping piece are unanimous with the action bars orientation of one of them clamping piece in the clamping piece of both sides, wherein the action bars of middle clamping piece can drive middle clamping piece 180 rotations in the vertical plane.
At present, a method for detecting sugar content of corn stalks by adopting a near infrared spectrum is provided, wherein a model of the sugar content of the corn stalks and a near infrared spectrum on a corn stalk section is established, the sugar content of the corn stalks to be detected is measured by applying the model, and no matter the method is used in the process of establishing the model or the process of applying the model, seven sections of the corn stalks need to be scanned by near infrared spectrum one by one, and the seven sections are respectively two new cross sections obtained by cross sections at two ends of a corn stalk sample and two new cross sections obtained by cross sections of different parts of the corn stalk sample. In the prior art, the method for detecting the sugar content of the corn stalks by adopting the near infrared spectrum has the following defects: only one section can be scanned each time, after one section is scanned, the scanning is stopped, the sample is taken out and replaced by one section, and then the scanning is performed, so that the operation is complex and the scanning efficiency is low.
The principle of the scheme is as follows: the method for detecting the sugar content of the corn stalk by adopting the near infrared spectrum needs to scan seven sections of the corn stalk sample, namely two new sections obtained by two cross sections of two ends of the corn stalk sample and two cross sections of different parts of the corn stalk sample, and longitudinal sections of three sections of the corn stalk sample. Therefore, the two ends of the corn stalk are cut off firstly to meet the length requirement of the corn stalk, a corn stalk sample is formed, meanwhile, the cross sections of the two ends of the corn stalk sample are cut, then two new cross sections are obtained through twice cross cutting, and finally, the corn stalk samples of three sections are longitudinally cut, and the longitudinal sections of the three sections are cut.
The method comprises the steps of placing the longitudinal sections of corn stalk samples of each section in a sample introduction box through a side door, placing the corn stalk samples of each section in the sample introduction box through a side door, clamping the corn stalk samples of the middle section in clamping parts of middle clamping pieces, wherein one end of each corn stalk sample of the middle section faces upwards (the two end faces are new cross sections), clamping the corn stalk samples of the two side sections in the clamping parts of the two side clamping pieces, enabling the cross sections of the two ends of each corn stalk sample to face upwards, scanning the cross sections of the two ends and one of the new cross sections, driving the middle clamping piece to rotate 180 degrees in a vertical plane through an operation rod after scanning, enabling the other new cross section to face upwards, scanning the other new cross section again, driving the three clamping pieces to be separated from the corn stalk samples through three operation rods, and gradually separating the longitudinal sections of the corn stalk samples of the three sections which are originally in the same after clamping and binding the corn stalk samples of the three sections without clamping pieces, namely, enabling the longitudinal sections of each corn stalk sample of the two sections to face upwards, and finally scanning the whole corn stalk samples of the three sections to be scattered.
The beneficial effect of this scheme lies in: in the scanning process of seven sections of the corn stalk sample, the corn stalk sample of three sections is only required to be placed into the second sample box once, the operation rod of the middle clamping piece is only required to be operated once in the process, the three operation rods are respectively operated to drive the three clamping pieces to be separated from the corn stalk sample of each section, and therefore all scanning of the seven sections of the whole corn stalk sample can be completed.
Further, the end part of each operating rod extends out of the second sample box, a rotating nut in threaded connection with the end part of each operating rod is arranged at the position of the second sample box at the end part of each operating rod, the inner end surface of the rotating nut abuts against the outer wall of the second sample box, and an anti-rotation structure is arranged between the second sample box and each operating rod.
The beneficial effects are that: an anti-rotation structure is arranged between the second sample box and each operating rod, so that the operating rods are not driven to rotate when the rotating nuts rotate, and the operating rods only do linear motion, so that the clamping pieces are driven to be separated from the corn stalk samples.
Further, the anti-rotation structure comprises a positioning strip arranged on the outer wall of the second sample box, a guide groove consistent with the movement direction of the operating rod is formed in the positioning strip, and a guide strip is arranged at the end part of the operating rod and can slide in the guide groove.
The beneficial effects are that: through setting up of locating strip and gib block, realize the direction of action bars when the motion to avoid action bars to rotate along with swivel nut.
Further, the positioning strip matched with the middle operating rod is an elastic positioning strip.
The beneficial effects are that: the locating strip matched with the middle operating rod is designed into an elastic locating strip, so that when the locating strip is required to guide, the locating strip stretches into the guide groove on the locating strip, and when the locating strip is not required to guide, namely the operating rod is required to rotate along with the rotating nut, the locating strip is withdrawn from the guide groove on the locating strip when the middle clamping piece is driven to rotate 180 degrees, and the limit on the operating rod is relieved.
Further, the corn harvester further comprises three groups of linkage jacking structures with the same structure, each group of linkage jacking structure comprises a first jacking rod arranged on the side face of the operation rod upwards, a first inclined plane is arranged on the end face of the first jacking rod, a vertical guide groove is formed in the inner wall of the sample box, a second jacking rod capable of sliding up and down along the vertical guide groove is arranged in the vertical guide groove, a second inclined plane capable of propping against the first inclined plane is arranged at the lower end of the second jacking rod, a horizontal guide hole is formed in the top of the second sample box, the horizontal guide hole is communicated with the guide groove, the upper end of the second jacking rod stretches into the horizontal guide hole, a third inclined plane is arranged at the upper end of the second jacking rod, a third jacking rod capable of sliding along the horizontal guide hole is arranged in the horizontal guide hole, a fourth inclined plane capable of propping against the third inclined plane is arranged at the right end of the third jacking rod, a fifth inclined plane is arranged at the left end of the third jacking rod, a vertical guide hole is formed in the top of the second sample box, the vertical guide hole is communicated with the horizontal guide hole, a vertical fourth jacking rod is arranged in the vertical guide hole, and the upper end of the fourth jacking rod is propped against the fifth inclined plane so as to be forced to stretch into the lower end of the corn stalk.
The principle of the design scheme is as follows:
when the operating rod stretches out of the second sample box, the first inclined surface of the first ejector rod is propped against the second inclined surface of the second ejector rod, the second ejector rod is forced to move upwards, the third inclined surface at the upper end of the second ejector rod is gradually propped against the fourth inclined surface of the third ejector rod, and then the third ejector rod is forced to move leftwards, so that the fifth inclined surface of the third ejector rod is propped against the sixth inclined surface at the upper end of the fourth ejector rod, and then the fourth ejector rod is forced to move downwards, and therefore the edge part of the fourth ejector rod is contacted with two longitudinal sections of a corn stalk sample in each section and the two longitudinal sections are forced to be separated, and therefore random dumping of the corn stalk sample when the clamping piece is separated from the corn stalk sample is avoided.
The beneficial effect of this design scheme lies in:
the corn stalk sample scanning device has the advantages that extra power is not required to be added, and in the process that the operating rod extends outwards, the separation of two longitudinal sections of the corn stalk sample is realized, so that the scanning of the longitudinal sections of the corn stalk sample in each section is smoother.
Further, a reset spring is arranged between the fourth ejector rod and the top of the second sample box.
The beneficial effects are that: by arranging the reset spring, the automatic reset of the fourth ejector rod can be realized, the next action is not influenced, and the fourth ejector rod is tensioned.
Further, the bottom parallel arrangement of second sample box has three standing grooves, and three standing grooves are located three clamping piece respectively under, and the orientation of three standing grooves all is perpendicular to the opening direction of the clamping part of every clamping piece.
The beneficial effects are that: the setting of standing groove, the effect has two, and one is the in-process that the cornstalk sample at three district scatters, plays the effect of direction to the cornstalk sample of every district, makes the cornstalk sample of every district can not interfere each other at the in-process that scatters, and only can scatter along the direction of guide slot, and another is the in-process that the clamping piece is throw off the cornstalk sample, plays the effect of location to the cornstalk sample, makes the cornstalk sample can not follow the clamping piece easily and emptys, can make clamping piece and cornstalk sample break off smoothly.
Further, the liquid chromatograph of the corn stalk sugar determination device adopts a Class-vp data processing system.
Drawings
FIG. 1 is a schematic diagram showing the structure of an embodiment of a corn stalk sugar determination apparatus according to the present application.
Fig. 2 is a schematic diagram of a linkage jack-up structure in an embodiment of the corn stalk sugar determination device of the application.
Description of the embodiments
The following is a further detailed description of the embodiments:
reference numerals in the drawings of the specification include: the device comprises a second sample box 1, a clamping piece 2, a placing groove 3, an operating rod 4, a rotating nut 5, a guide bar 6, a positioning bar 7, a first ejector rod 8, a first inclined plane 9, a second ejector rod 10, a second inclined plane 11, a third inclined plane 12, a third ejector rod 13, a fourth inclined plane 14, a fifth inclined plane 15, a fourth ejector rod 16, a sixth inclined plane 17, a blade part 18, a reset spring 19, a corn stalk sample 20, a side door 21, a vertical guide groove 22, a horizontal guide hole 23 and a vertical guide hole 24.
The embodiment discloses a corn stalk sugar determination device, including first sample box and second sample box.
The first sample box is used for containing filtered sugar extracting solution, and the sugar extracting solution is as follows: removing female ear, leaf and leaf sheath from ear position section, ear position upper section and ear position lower section for 3 sections, adding liquid nitrogen, quick freezing, preserving at-80deg.C for sugar content measurement, quick freezing, adding liquid nitrogen into the mixture, grinding into powder, collecting 0.1-0.2 g, adding 80% ethanol 2 mL, mixing, leaching at 80deg.C for 2 hr, centrifuging at 12 r/min for 10 min, collecting supernatant 1 mL, concentrating and drying, dissolving precipitate with 1 mL ultrapure water, and filtering.
The corn stalk sugar determination device in the embodiment further comprises a liquid chromatograph matched with the first sample box, wherein the liquid chromatograph comprises a RID-10A differential refraction detector, and the liquid chromatograph adopts a Class-vp data processing system. Operating conditions of the chromatographic column of the liquid chromatograph: the column temperature is 40 ℃, the flow rate is 1.0 mL/min, and the volume of each sample injection is 20 mu L. The liquid chromatograph calculates the content of sugar according to the peak area of the sample and the standard curve of each sugar, and the liquid chromatograph is repeated for 3 times, and each repetition is ensured to be an independent sample. The total sugar content was calculated as the sum of fructose, glucose and sucrose contents.
In this embodiment, the second sample box is configured to hold a corn stalk sample with multiple tangential planes; as shown in fig. 1 and 2: the embodiment discloses a corn stalk sugar determination device, which is provided with a Pbs detector and OPUS analysis software, and uses the OPUS analysis software to carry out mathematical treatment on absorption spectrum data, and adopts near infrared spectrum to determine sugar content of a corn stalk sample 20. The actual sugar content of a plurality of samples is actually measured by a chemical method (such as a phenol method, an anthrone colorimetric method and the like), near infrared spectrums of the sections of the plurality of samples are scanned, and a corresponding relation between the actual sugar content and the near infrared spectrums of the sections of the plurality of samples is established. In the modeling process, abnormal samples are removed, a sample set and a test set are established for the residual sample set from which the abnormal samples are removed, the near infrared spectrum is preprocessed and the characteristic wavelength is screened, and a near infrared spectrum model of the sugar content of the corn stalks is established by adopting a partial least square method. The above method is prior art and will not be described here in detail.
In the process of establishing the model or in the process of applying the model, near infrared spectrum scanning is needed to be carried out on seven sections of the corn stalk one by one, wherein the seven sections are respectively two new cross sections obtained by cross sections at two ends of the corn stalk sample 20, two new cross sections obtained by cross sections of different parts of the corn stalk sample 20 and longitudinal sections of three sections of the corn stalk sample 20.
In the corn stalk sugar determination device of the embodiment, unlike the rotary sample cup adopted in the prior art, the embodiment designs a second sample box 1, a side door 21 is opened on the side surface of the second sample box 1, and the side door 21 is a side door 21 with a light leakage prevention function, such as the side door 21 is gold-plated.
Three clamping pieces 2 used for clamping the corn stalk sample 20 are arranged in the second sample box 1, three placing grooves 3 are arranged at the bottom of the second sample box 1 in parallel, the three placing grooves 3 are respectively located under the three clamping pieces 2, and the directions of the three placing grooves 3 are perpendicular to the opening direction of the clamping part of each clamping piece 2. Each clamping piece 2 is provided with an inwards concave clamping part, the clamping part is designed to be just capable of being in contact with the periphery of the corn stalk, the corn stalk sample 20 cannot be clamped too tightly, and meanwhile, the clamping piece is designed to be of a piece-shaped structure, so that the clamping piece is conveniently separated from the surface of the corn stalk sample 20, and the corn stalk sample 20 cannot be separated or can be driven to topple due to too tight clamping or too large clamping force.
In this embodiment, middle clamping piece 2 and both sides clamping piece 2 dislocation set, every clamping piece 2 all is provided with the action bars 4 that can drive clamping piece 2 to throw off maize stalk sample 20 in the one side of clamping part away from, the action bars 4 of middle clamping piece 2 are unanimous with the action bars 4 of one of them clamping piece 2 in the clamping piece 2 of both sides, the tip of every action bars 4 all stretches out second sample box 1, the position that lies in the tip of every action bars 4 at second sample box 1 all is equipped with the swivel nut 5 with action bars 4 tip threaded connection, swivel nut 5's interior terminal surface offsets with second sample box 1 outer wall, all be equipped with anti-rotation structure between second sample box 1 and every action bars 4.
In this embodiment, the anti-rotation structure includes a positioning strip 7 disposed on an outer wall of the second sample box 1, where the positioning strip 7 matched with the intermediate operating rod 4 is an elastic positioning strip 7. The positioning strip 7 is provided with a guide groove consistent with the movement direction of the operating rod 4, the end part of the operating rod 4 is provided with a guide strip 6, and the guide strip 6 can slide in the guide groove. The positioning strip 7 matched with the middle operating rod 4 is an elastic positioning strip 7, when the middle operating rod 4 does not need to be guided, namely, the middle operating rod 4 needs to rotate along with the rotating nut 5, the operating rod 4 drives the middle clamping piece 2 to rotate 180 degrees, the guiding strip 6 is withdrawn from the guiding groove on the positioning strip 7, and the limitation on the operating rod 4 is relieved.
In this embodiment, the device further includes three groups of linkage pushing structures with the same structure, each group of linkage pushing structures includes a first push rod 8 disposed on a side face of the operation rod 4 and facing upwards, a first inclined plane 9 is disposed on an end face of the first push rod 8, a vertical guide groove 22 is formed in an inner wall of the second sample box 1, a second push rod 10 capable of sliding up and down along the vertical guide groove 22 is disposed in the vertical guide groove 22, a second inclined plane 11 capable of propping against the first inclined plane 9 is disposed at a lower end of the second push rod 10, and when the first inclined plane 9 of the first push rod 8 props against the second inclined plane 11 at a lower end of the second push rod 10, the second push rod 10 can be forced to move up along the vertical guide groove 22.
The top of the second sample box 1 is provided with a horizontal guide hole 23, the horizontal guide hole 23 is communicated with the guide groove, the upper end of the second ejector rod 10 stretches into the horizontal guide hole 23, the upper end of the second ejector rod 10 is provided with a third inclined surface 12, a third ejector rod 13 capable of sliding along the horizontal guide hole 23 is arranged in the horizontal guide hole 23, the right end of the third ejector rod 13 is provided with a fourth inclined surface 14 capable of propping against the third inclined surface 12, and after the third inclined surface 12 of the second ejector rod 10 is propped against the fourth inclined surface 14 of the third ejector rod 13, the second ejector rod 10 can force the third ejector rod 13 to move leftwards along the horizontal guide hole 23.
The left end of the third ejector rod 13 is provided with a fifth inclined plane 15, the top of the second sample box 1 is provided with a vertical guide hole 24, the vertical guide hole 24 is communicated with a horizontal guide hole 23, a vertical fourth ejector rod 16 is arranged in the vertical guide hole 24 in a penetrating mode, the upper end of the fourth ejector rod 16 is provided with a sixth inclined plane 17 which can be propped against the fifth inclined plane 15 to force the fourth ejector rod 16 to slide downwards, and the lower end of the fourth ejector rod 16 is provided with a blade part 18 which can extend into the longitudinal section of the corn stalk. When the fourth ejector rod 16 moves downwards along the vertical guide hole 24, the two longitudinal sections of the corn stalk sample 20 of one section which are originally opposite together can be separated by the blade part 18 arranged at the lower end of the fourth ejector rod 16, so that the corn stalk sample 20 of one section is smoothly scattered without the constraint of the clamping piece 2, the longitudinal section faces upwards, and the scanning is convenient.
A return spring 19 is arranged between the fourth ejector rod 16 and the top of the second sample box 1, and the return spring 19 is a tension spring. The concrete structure is that a horizontal mounting strip is arranged on the circumference of a fourth ejector rod 16, and a tension spring is arranged between the horizontal mounting strip and the inner wall of the top of the second sample box 1.
The foregoing is only illustrative of the present application and is not intended to be limiting of the practice of the application, as those of ordinary skill in the art will appreciate from the teachings of the present application that certain typical well-known structures or well-known methods may be incorporated in their own right and practiced by those of ordinary skill in the art. It should be noted that modifications and improvements can be made by those skilled in the art without departing from the structure of the present application, and these should also be considered as the scope of the present application, which does not affect the effect of the implementation of the present application and the utility of the patent. The protection scope of the present application is subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.
Claims (7)
1. Corn stalk sugar determination device, including first sample box and second sample box, its characterized in that: the first sample box is used for containing filtered sugar extract, and the second sample box is used for containing corn stalk samples with a plurality of sections; the liquid chromatograph is matched with the first sample box for use, comprises a RID-10A differential refraction detector, and calculates the content of sugar according to the peak area of the sample and the standard curve of each sugar; the sugar extract is as follows: removing female ear, leaf and leaf sheath from 3 segments of ear segment, ear upper segment and ear lower segment, adding liquid nitrogen, quick freezing, adding liquid nitrogen into the mixture, grinding into powder, adding 0.1-0.2. 0.2 g, adding 80% ethanol 2 mL, mixing, leaching at 80deg.C for 2 hr, centrifuging at 12 r/min for 10 min, concentrating and drying 1 mL supernatant, dissolving precipitate with 1 mL ultrapure water, and filtering to obtain the final product; a side door is arranged on the side surface of the second sample box, three clamping pieces for clamping the corn stalk sample are arranged in the second sample box, each clamping piece is provided with an inward concave clamping part, the middle clamping piece and the clamping pieces on two sides are arranged in a staggered mode, one side, opposite to the clamping parts, of each clamping piece is provided with an operating rod for driving the clamping piece to separate from the corn stalk sample, the operating rod of the middle clamping piece is consistent with the operating rod of one clamping piece on the two sides, and the operating rod of the middle clamping piece drives the middle clamping piece to rotate 180 degrees in a vertical plane; the device also comprises three groups of linkage jacking structures with the same structure, each group of linkage jacking structure comprises a first ejector rod arranged on the side face of the operating rod and upwards, the end face of the first ejector rod is provided with a first inclined plane, the inner wall of the sample box is provided with a vertical guide groove, a second ejector rod sliding up and down along the vertical guide groove is arranged in the vertical guide groove, the lower end of the second ejector rod is provided with a second inclined plane propped against the first inclined plane, the top of the second sample box is provided with a horizontal guide hole, the horizontal guide hole is communicated with the guide groove, the upper end of the second ejector rod extends into the horizontal guide hole, the upper end of the second ejector rod is provided with a third inclined plane, the third ejector rod sliding along the horizontal guide hole is arranged in the horizontal guide hole, the right end of the third ejector rod is provided with a fourth inclined plane propped against the third inclined plane, the left end of the third ejector rod is provided with a fifth inclined plane, the top of the second sample box is provided with a vertical guide hole, the vertical guide hole is communicated with the horizontal guide hole, a vertical fourth ejector rod is arranged in the vertical guide hole in a penetrating mode, the upper end of the fourth ejector rod is provided with a sixth inclined plane propped against the fifth inclined plane to force the fourth ejector rod to slide downwards, and the lower end of the fourth ejector rod is provided with a blade part stretching into a longitudinal section of a corn stalk.
2. The corn stalk sugar determination apparatus according to claim 1, wherein: the end part of each operating rod extends out of the second sample box, a rotating nut in threaded connection with the end part of each operating rod is arranged at the position of the second sample box at the end part of each operating rod, the inner end surface of the rotating nut abuts against the outer wall of the second sample box, and an anti-rotation structure is arranged between the second sample box and each operating rod.
3. The corn stalk sugar determination apparatus according to claim 2, wherein: the anti-rotation structure comprises a positioning strip arranged on the outer wall of the second sample box, a guide groove consistent with the movement direction of the operating rod is formed in the positioning strip, and the end part of the operating rod is provided with the guide strip which slides in the guide groove.
4. A corn stalk sugar determination apparatus according to claim 3, wherein: the positioning strip matched with the middle operating rod is an elastic positioning strip.
5. The corn stalk sugar determination apparatus according to claim 1, wherein: and a reset spring is arranged between the fourth ejector rod and the top of the second sample box.
6. The corn stalk sugar determination apparatus according to claim 1, wherein: the bottom parallel arrangement of second sample box has three standing groove, and three standing groove is located three clamping piece under respectively, and the orientation of three standing groove all is perpendicular to the opening direction of the clamping part of every clamping piece.
7. The corn stalk sugar determination apparatus according to claim 1, wherein: the liquid chromatograph adopts a Class-vp data processing system.
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