CN115177695B - Traditional Chinese medicine composition for whitening skin and removing freckles and application thereof - Google Patents
Traditional Chinese medicine composition for whitening skin and removing freckles and application thereof Download PDFInfo
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- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
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Abstract
The invention discloses a traditional Chinese medicine composition for whitening skin and removing freckles, which is prepared from the following raw materials in parts by weight: 8-10 parts of ginseng extract, 8-10 parts of dittany bark extract or bletilla striata extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of dwarf lilyturf tuber extract, 2-4 parts of licorice root extract, and 2-4 parts of camellia extract or rose extract. The traditional Chinese medicine composition provided by the invention not only acts on melanocytes, but also can inhibit inflammatory reaction, repair skin barrier, whiten and remove spots in multiple dimensions.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines and cosmetics, and particularly relates to a traditional Chinese medicine composition for whitening skin and removing freckles and application thereof.
Background
In recent years, as the aesthetic level of appearance of skin is improved, more eastern women pay more attention to their own complexion. Some loving women have difficulty in having white skin and tender appearance due to the influence of various factors. The dull dry water-deficient skin is caused by a plurality of reasons, such as congenital genetic factors, acquired sub-health factors, disease factors, dietary factors, environmental factors and the like. Particularly for women entering middle-aged and elderly people, various in-vivo and in-vitro problems are accumulated, melanin deposition, melanocyte metabolism disorder and the like are more likely to occur, and a series of skin problems such as freckle, senile plaque, chloasma, skin blackening, dark complexion, wax yellow and the like are presented. Therefore, in China society believing "one hides one hundred chores", the problem of female health whitening is always a beauty problem which is attracting attention.
Many factors affect chloasma or pigmentation, for example: endocrine disorders, local inflammatory reactions, and even barrier damage are all related, so preventing melanogenesis by acting on melanocytes alone does not solve the skin problem of increased melanosis well. Many products are effective on a single principle, such as single inhibition of tyrosinase activity, or single blocking of melanin from running to epidermis, etc., and have slow effect and limited degree of effect.
Most of the prior whitening products are singly acted on melanocytes or melanin products, so that the improvement period of the skin color of consumers is long and the whitening products are easy to repeat.
Disclosure of Invention
In view of the above, the technical problem to be solved by the invention is to provide a traditional Chinese medicine composition for whitening skin and removing freckles and a preparation method thereof.
In a first aspect, the invention provides a traditional Chinese medicine composition for whitening skin and removing freckles, which is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of dittany bark extract or bletilla striata extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of dwarf lilyturf tuber extract, 2-4 parts of licorice root extract, and 2-4 parts of camellia extract or rose extract.
Optionally, the traditional Chinese medicine composition for whitening skin and removing freckles is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of dittany bark extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of dwarf lilyturf tuber extract, 2-4 parts of glabrous greenbrier rhizome extract and 2-4 parts of camellia extract.
Optionally, the traditional Chinese medicine composition for whitening skin and removing freckles is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of dittany bark extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of dwarf lilyturf tuber extract, 2-4 parts of glabrous greenbrier rhizome extract and 2-4 parts of rose extract.
Optionally, the traditional Chinese medicine composition for whitening skin and removing freckles is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of bletilla striata extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of dwarf lilyturf tuber extract, 2-4 parts of licorice root extract and 2-4 parts of camellia extract.
Optionally, the traditional Chinese medicine composition for whitening skin and removing freckles is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of bletilla striata extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of dwarf lilyturf tuber extract, 2-4 parts of glabrous greenbrier rhizome extract and 2-4 parts of rose extract.
In a second aspect, the invention provides application of the traditional Chinese medicine composition in preparation of cosmetics for whitening skin and removing freckles.
Optionally, the cosmetic comprises one or more of skin cleaning, lotion, cream, essence, isolation, sun protection, and foundation.
Compared with the prior art, the invention provides a traditional Chinese medicine composition for whitening skin and removing freckles, which is prepared from the following raw materials in parts by weight: 8-10 parts of ginseng extract, 8-10 parts of dittany bark extract or bletilla striata extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of dwarf lilyturf tuber extract, 2-4 parts of licorice root extract, and 2-4 parts of camellia extract or rose extract. The invention has the effects of inhibiting tyrosinase activity, accelerating melanin metabolism and reducing generated melanin through multiple whitening paths. The factors forming melanin and chloasma are also involved in inflammatory reactions and impaired skin barriers, and it is also important to combat inflammation and repair barriers. The traditional Chinese medicine composition provided by the invention not only acts on melanocytes, but also can inhibit inflammatory reaction, repair skin barrier, whiten and remove spots in multiple dimensions.
Drawings
FIG. 1 is a side view of the body surface melanin inhibiting effect of a normal control group, a 1% group, and a 2% group of zebra fish embryos;
FIG. 2 is a bar graph of body surface melanin content of zebra fish embryos of normal control, 1% and 2% groups;
FIG. 3 is a side view of the length and budding amount of the new blood vessels of the zebra fish embryos of the normal control group, the 1% group and the 2% group;
FIG. 4 is a bar graph of the germination rate of zebra fish embryos from the normal control, 1% and 2% groups;
FIG. 5 is a bar graph of the length of the new blood vessels of the zebra fish embryos of the normal control group, the 1% group, and the 2% group.
Detailed Description
The invention provides a traditional Chinese medicine composition for whitening skin and removing freckles, wherein components for resisting inflammation and repairing barriers are added into the traditional Chinese medicine composition, the traditional Chinese medicine composition has good effects on inflammatory pigmentation and skin barrier damage type freckles, and the traditional Chinese medicine whitening and freckles-fading formula which is purely natural and has multiple functions is provided.
The traditional Chinese medicine composition provided by the invention is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of dittany bark extract or bletilla striata extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of dwarf lilyturf tuber extract, 2-4 parts of licorice root extract, and 2-4 parts of camellia extract or rose extract.
In some specific embodiments of the invention, the traditional Chinese medicine composition for whitening skin and removing freckles is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of dittany bark extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of dwarf lilyturf tuber extract, 2-4 parts of glabrous greenbrier rhizome extract and 2-4 parts of camellia extract.
In some specific embodiments of the invention, the traditional Chinese medicine composition for whitening skin and removing freckles is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of dittany bark extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of dwarf lilyturf tuber extract, 2-4 parts of glabrous greenbrier rhizome extract and 2-4 parts of rose extract.
In some specific embodiments of the invention, the traditional Chinese medicine composition for whitening skin and removing freckles is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of bletilla striata extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of dwarf lilyturf tuber extract, 2-4 parts of licorice root extract and 2-4 parts of camellia extract.
In some specific embodiments of the invention, the traditional Chinese medicine composition for whitening skin and removing freckles is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of bletilla striata extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of dwarf lilyturf tuber extract, 2-4 parts of glabrous greenbrier rhizome extract and 2-4 parts of rose extract.
The traditional Chinese medicine composition for whitening skin and removing freckles provided by the invention comprises 8-10 parts of ginseng extract, preferably 8, 8.5, 9, 9.5, 10 or any value between 8 and 10 parts.
The traditional Chinese medicine composition for whitening skin and removing freckles provided by the invention also comprises 8-10 parts of cortex dictamni extract or 8-10 parts of bletilla striata extract, preferably 8, 8.5, 9, 9.5, 10 or any value between 8 and 10 parts.
The traditional Chinese medicine composition for whitening skin and removing freckles provided by the invention also comprises 6-8 parts of chamomile extract, preferably 6, 6.5, 7, 7.5, 8 or any value between 6 and 8 parts.
The traditional Chinese medicine composition for whitening skin and removing freckles also comprises 4-6 parts of lucid ganoderma extract, preferably 4, 4.5, 5, 5.5 and 6 or any value between 4 and 6 parts.
The traditional Chinese medicine composition for whitening skin and removing freckles also comprises 4-6 parts of pseudo-ginseng extract, preferably 4, 4.5, 5, 5.5 and 6 parts or any value between 4 and 6 parts.
The traditional Chinese medicine composition for whitening skin and removing freckles also comprises 4-6 parts of saffron extract, preferably 4, 4.5, 5, 5.5 and 6 or any value between 4 and 6 parts.
The traditional Chinese medicine composition for whitening skin and removing freckles also comprises 4-6 parts of hairyvein agrimony extract, preferably 4, 4.5, 5, 5.5, 6 or any value between 4 and 6 parts.
The traditional Chinese medicine composition for whitening skin and removing freckles also comprises 4-6 parts of ophiopogon root extract, preferably 4, 4.5, 5, 5.5 and 6 or any value between 4 and 6 parts.
The traditional Chinese medicine composition for whitening skin and removing freckles also comprises 2-4 parts of licorice extract, preferably 2, 2.5, 3, 3.5, 4 or any value between 2 and 4 parts.
The traditional Chinese medicine composition for whitening skin and removing freckles provided by the invention also comprises 2-4 parts of camellia extract or 2-4 parts of rose extract, preferably 2, 2.5, 3, 3.5, 4 or any value between 2 and 4 parts.
The invention also provides application of the traditional Chinese medicine composition in preparing cosmetics for whitening skin and removing freckles.
Wherein the cosmetic comprises one or more of skin cleaning, lotion, cream, essence, isolation, sun protection, and foundation.
The invention has the effects of inhibiting tyrosinase activity, accelerating melanin metabolism and reducing generated melanin through multiple whitening paths. The factors forming melanin and chloasma are also involved in inflammatory reactions and impaired skin barriers, and it is also important to combat inflammation and repair barriers. The traditional Chinese medicine composition provided by the invention not only acts on melanocytes, but also can inhibit inflammatory reaction, repair skin barrier, whiten and remove spots in multiple dimensions.
In order to further understand the present invention, the following examples are provided to illustrate the traditional Chinese medicine composition for whitening skin and removing freckle and the application thereof, and the scope of the present invention is not limited by the following examples.
In the following examples, ginseng extract, dittany bark extract, bletilla striata extract, chamomile extract, ganoderma lucidum extract, pseudo-ginseng extract, saffron extract, hairyvein agrimony extract, ophiopogon root extract, licorice root extract, camellia extract, rose extract, ligusticum wallichii extract, polygonum cuspidatum extract and lily extract are all commercially available.
Example 1
9 parts of ginseng extract, 9 parts of dittany bark extract, 7 parts of chamomile extract, 5 parts of lucid ganoderma extract, 5 parts of pseudo-ginseng extract, 5 parts of saffron extract, 5 parts of hairyvein agrimony extract, 5 parts of dwarf lilyturf tuber extract, 3 parts of licorice root extract and 3 parts of camellia extract. Mixing the traditional Chinese medicine extracts according to a proportion, and dispersing the mixture in 560 parts of water to obtain the traditional Chinese medicine composition stock solution for whitening skin and removing freckles.
Comparative example 1
9 parts of ginseng extract, 9 parts of dittany bark extract, 7 parts of chamomile extract, 5 parts of ganoderma lucidum extract, 5 parts of pseudo-ginseng extract, 5 parts of saffron extract, 5 parts of hairyvein agrimony extract, 5 parts of dwarf lilyturf tuber extract and 3 parts of camellia extract.
Comparative example 1 based on example 1, the Glycyrrhiza glabra extract was removed, the other conditions being unchanged.
Comparative example 2
9 parts of ginseng extract, 9 parts of dittany bark extract, 7 parts of chamomile extract, 5 parts of pseudo-ginseng extract, 5 parts of saffron extract and 3 parts of camellia extract.
Comparative example 2 based on example 1, the ganoderma lucidum extract, the agrimony extract, the ophiopogon root extract were removed, and the other conditions were unchanged.
Comparative example 3
9 parts of ginseng extract, 9 parts of dittany bark extract, 7 parts of chamomile extract, 5 parts of szechuan lovage rhizome extract, 5 parts of pseudo-ginseng extract, 5 parts of saffron extract, 5 parts of giant knotweed extract, 5 parts of lily extract, 3 parts of licorice root extract and 3 parts of camellia extract.
Comparative example 3 based on example 1, the glossy ganoderma extract was replaced with the Ligusticum wallichii extract, the hairyvein agrimony extract was replaced with the Polygonum cuspidatum extract, the radix Ophiopogonis extract was replaced with the Lily extract, and the other conditions were unchanged.
Comparative example 4
5 parts of ginseng extract, 5 parts of dittany bark extract, 3 parts of chamomile extract, 3 parts of lucid ganoderma extract, 3 parts of pseudo-ginseng extract, 3 parts of saffron extract, 3 parts of hairyvein agrimony extract, 3 parts of dwarf lilyturf tuber extract, 3 parts of liquorice root extract and 3 parts of camellia flower extract
Comparative example 4 the parts of ginseng extract, dittany bark extract, chamomile extract, ganoderma lucidum extract, pseudo-ginseng extract, saffron extract, hairyvein agrimony extract and ophiopogon root extract were reduced on the basis of example 1.
Example 2
9 parts of ginseng extract, 9 parts of dittany bark extract, 7 parts of chamomile extract, 5 parts of lucid ganoderma extract, 5 parts of pseudo-ginseng extract, 5 parts of saffron extract, 5 parts of hairyvein agrimony extract, 5 parts of dwarf lilyturf tuber extract, 3 parts of licorice root extract and 3 parts of rose extract. Mixing the traditional Chinese medicine extracts according to a proportion, and dispersing the mixture in 560 parts of water to obtain the traditional Chinese medicine composition stock solution for whitening skin and removing freckles.
Example 3
9 parts of ginseng extract, 9 parts of bletilla striata extract, 7 parts of chamomile extract, 5 parts of lucid ganoderma extract, 5 parts of pseudo-ginseng extract, 5 parts of saffron extract, 5 parts of hairyvein agrimony extract, 5 parts of dwarf lilyturf tuber extract, 3 parts of glabrous greenbrier rhizome extract and 3 parts of camellia extract. Mixing the traditional Chinese medicine extracts according to a proportion, and dispersing the mixture in 560 parts of water to obtain the traditional Chinese medicine composition stock solution for whitening skin and removing freckles.
Example 4
9 parts of ginseng extract, 9 parts of bletilla striata extract, 7 parts of chamomile extract, 5 parts of lucid ganoderma extract, 5 parts of pseudo-ginseng extract, 5 parts of saffron extract, 5 parts of hairyvein agrimony extract, 5 parts of dwarf lilyturf tuber extract, 3 parts of glabrous greenbrier rhizome extract and 3 parts of rose extract. Mixing the traditional Chinese medicine extracts according to a proportion, and dispersing the mixture in 560 parts of water to obtain the traditional Chinese medicine composition stock solution for whitening skin and removing freckles.
Test set one
The whitening and microcirculation efficacy of the traditional Chinese medicine composition stock solution prepared in the example 1 is evaluated through zebra fish embryo experiments.
1. Reagents and materials
24-well plates, 5ml centrifuge tubes, plastic pipettes, petri dishes, pipette, glass slides, holt Buffer solution, methylcellulose.
2. Instrument for measuring and controlling the intensity of light
Constant temperature incubator, zebra fish culture system, upright fluorescence microscope, continuous zoom stereoscopic microscope.
3. Test design
3.1 test group
The test was performed in a total of 3 groups, namely, a normal control group, a 2% group and a 1% group, wherein the normal control group was not added with the stock solution of the traditional Chinese medicine composition in the culture dish, the 2% group was added with the stock solution of the traditional Chinese medicine composition diluted to 2% in the culture dish, namely, diluted to 2% based on the stock solution of example 1, and the 1% group was added with the stock solution of the traditional Chinese medicine composition diluted to 1% in the culture dish, namely, diluted to 1% based on the stock solution of example 1.
3.2 packet design
Three auxiliary wells were set for each concentration, with 20 embryos per well.
Table 1 dose design table
4. Test method
4.1 whitening efficacy verification
1) Day0, add 2% and 1% diluted stock solutions of the Chinese medicinal composition to 2% and 1% group petri dishes, respectively.
2) Dayl. The wild zebra fish 24hpf embryos of AB line were collected 180 and added to the petri dish, and the embryos were grouped according to 20 embryos per group, and each group was repeated three times, with a specific grouping scheme being shown in 3.2.
3) Day3, observing the development state of the melanin on the body surface of the embryo, selecting representative 2-3 tails for each group to record photographing, and carrying out quantitative analysis.
4) Day4-Day6, data analysis and processing.
4.2 microcirculation efficacy validation
1) Day0, add 2% and 1% diluted stock solutions of the Chinese medicinal composition to 2% and 1% group petri dishes, respectively.
2) Dayl, collecting 180 embryos of AB-line transgenic zebra fish 24hpf, adding into a culture dish, grouping according to 20 embryos of each group, and repeating each group three times, wherein the specific grouping scheme is shown in 3.2. After grouping, except for the normal control group, 2% and 1% groups were modeled with angiogenesis inhibitors, and zebra fish microcirculation injury models were constructed.
3) Day3, observing the microcirculation development state of the embryo, selecting a representative 2-3 tail for fluorescence microscopic imaging of each group, and carrying out quantitative analysis.
4) Day4-Day6, data analysis and processing.
5. Analysis of results
5.1 whitening efficacy verification
The procedure was followed as shown in 4.1, with deformity rates and mortality rates counted for the 2% and 1% groups after 48 hours, and the melanin content of the normal control group, 2% group and 1% group were compared.
The 2% group zebra fish embryo development is basically normal without obvious malformation, the body surface melanin is reduced compared with the normal control group, the 1% group embryo development is basically normal without obvious malformation, and the body surface melanin is reduced compared with the normal control group.
Referring to fig. 1, fig. 1 is a side view of the melanin inhibiting effect of the body surface of zebra fish embryos of normal control group, 1% group and 2% group. The area of the melanin plaque in the graph was counted. The melanin content of the 1% group is reduced (36+/-10)% compared with that of the normal control group, and the melanin content of the 1% group and the normal control group are obviously different (P < 0.05). The melanin content of the 2% group is reduced (66+/-10)% compared with that of the normal control group, and the melanin content of the 2% group and the melanin content of the normal control group are obviously different (P < 0.01).
Referring to table 2, table 2 shows the melanin contents (average value ± SE) of the normal control group, the 1% group, the 2% group, the normal control group, the melanin content of 1 ± 0.04,1% and the melanin content of 0.64 ± 0.10,2% group, the melanin content of 0.34 ± 0.10,1% group being significantly different from the normal control group (P < 0.05), and the 2% group being significantly different from the normal control group (P < 0.01).
TABLE 2 melanin content (average.+ -. SE) in normal control, 1% and 2% groups
Referring to fig. 2, fig. 2 is a bar graph of the body surface melanin content of zebra fish embryos of normal control group, 1% group, and 2% group. The melanin content of the 1% group is (64+ -10)% (P < 0.05) of the normal control group. The melanin content of the 2% group is (34+ -10)% (P < 0.01) of the normal control group.
5.2 microcirculation efficacy validation
The procedure was performed as shown in 4.2, and after 48 hours the malformation rate and mortality were counted for the 2% and 1% groups, and the length and sprouting rate of the new blood vessels were compared for the normal control group, the 2% group and the 1%.
2% of zebra fish embryos are basically normal in development without obvious deformity, the length and the budding rate of new blood vessels are increased compared with those of a normal control group, 1% of zebra fish embryos are basically normal in development without obvious deformity, and the length and the budding rate of new blood vessels are increased compared with those of the normal control group.
Referring to fig. 3, fig. 3 is a side view of the length and budding amount of zebra fish embryo neovascularization in the normal control group, 1% group, and 2% group.
And counting the sprouting rate of the blood vessels in the graph. The germination rate of the 1% group is increased (88+/-8)% compared with that of the normal control group, and the germination rate are extremely obviously different (P < 0.001). The germination rate of the 2% group is increased (92+/-2)% compared with that of the normal control group, and the germination rate are extremely obviously different (P < 0.001).
TABLE 3 budding rate (average.+ -. SE) of normal control group, 1% of test article, and 2% of test article
The 1% group was very significantly different from the normal control group (P < 0.001), and the 2% group was very significantly different from the normal control group (P < 0.001).
Referring to fig. 4, fig. 4 is a bar graph of the germination rate of zebra fish embryos from the normal control group, the 1% group, and the 2% group. Wherein, the germination rate of 1% group is (188+/-8)% (P < 0.001) of the normal control group. The germination rate of the 2% group is (192+/-2)% of that of the normal control group (P < 0.001).
TABLE 4 length of neovasculature in normal control group, test 1% group, test 2% group (average SE)
The 1% group had very significant differences from the normal control group (P < 0.001), and the 2% group had significant differences from the normal control group (P < 0.05).
Referring to fig. 5, fig. 5 is a bar graph of the length of the new blood vessels of zebra fish embryos of normal control group, 1% group, 2% group. The angiogenesis length of 1% group is (199+ -13)% (P < 0.001) of the normal control group, and the angiogenesis length of 2% group is (186+ -10)% (P < 0.05) of the normal control group.
The results show that, when the traditional Chinese medicine composition stock solution with the concentration diluted to 2% and 1% is added into the culture environment of 24hpf zebra fish embryos until the embryo development is 72hpf,2% of zebra fish embryos are basically normal and have no obvious deformity, the body surface melanin is reduced compared with the normal control group, and the angiogenesis length and the sprouting rate of blood vessels are both increased compared with the normal control group. The embryo development of 1% group is basically normal without obvious malformation, the melanin on the body surface is reduced compared with the normal control group, and the angiogenesis length and the budding rate of blood vessels are both increased compared with the normal control group. In conclusion, the raw liquid of the traditional Chinese medicine composition provided by the invention can effectively inhibit the melanin of the zebra fish embryo and promote capillary angiogenesis of the zebra fish embryo when the concentration is 2% and 1%. Experiments prove that the stock solution of the traditional Chinese medicine composition provided by the invention can effectively inhibit melanin generation, effectively promote capillary angiogenesis and has the potential of improving blood vessel circulation.
Test group II
The therapeutic effect of the stock solution of the traditional Chinese medicine composition on chloasma was systematically evaluated by using the stock solution of the traditional Chinese medicine composition prepared in example 1 on chloasma subjects.
110 subjects with chloasma are selected, skin care is carried out after facial cleaning every day, the traditional Chinese medicine composition stock solution is used for 2 times/d locally, the continuous use is carried out for 28d, and the curative effect observation is carried out after the treatment is finished.
Evaluation criteria:
(1) The inhibition rate of the neuraminidase: 4mL of a buffer solution (phosphoric acid-citric acid mclvains buffer solution) with pH of 6.8 is added to samples of the stock solutions of the traditional Chinese medicine compositions with concentrations of 0.2%, 0.4%, 0.8%, 1.6% and 2.0%, the mixture is preheated in a constant temperature water tank at 37 ℃ for 10 minutes, 4mL of an aqueous solution of the complex aminoacase (0.05 g/L mclvains buffer solution) is added, the mixture is fully stirred, the mixture is reacted at 37 ℃ for 15 minutes, the absorbance (A1) at 475nm is measured, a solution without the stock solution of the traditional Chinese medicine composition is additionally arranged, the absorbance (A2) is measured at 475nm according to the method, a blank control group is finally arranged, and neither the stock solution of the traditional Chinese medicine composition nor the solution of the complex aminoacase is added, and the absorbance (A3) is measured at 475nm according to the method.
The calculation formula of the inhibition rate of the neuraminidase is as follows: inhibition ratio = (A2-A1)/(A2-A3) ×100%
(2) The effective rate is as follows: the following 4 grades are proposed according to the first 2 curative effect evaluation standards in the "clinical diagnosis of chloasma and curative effect determination standard (revised manuscript in 2003)" (established in the skin disease professional committee pigment group of the Chinese and Western medicine combination society, 2013, month 12): (1) basic cure: visually, the area of the color spots subsides by more than 90 percent, and the color is basically disappeared; the index of decrease after treatment is more than or equal to 0.8. (2) The effect is shown: visually, the color spot area subsides by more than 60 percent, and the color is obviously lightened; the index of decrease after treatment is more than or equal to 0.5. (3) The method is effective: visually, the area of the color spots subsided by more than 30%, and the color was faded; the index of decrease after treatment is more than or equal to 0.3. (4) Invalidation: visual inspection, the area of the color spots subsides by less than 30 percent, and the color change is not obvious; the index of decrease after treatment is less than or equal to 0.
The total effective rate calculation formula is: total effective rate = (basic cure + significant effect + effective)/100 x 100%
The inhibition rate of the neuraminidase:
table 5 shows the inhibition ratios of the crude solutions of the Chinese medicinal compositions diluted to 0.2%, 0.4%, 0.8%, 1.6% and 2.0% in concentration.
TABLE 5 inhibition ratio of Chinese medicinal composition stock solutions diluted to different concentrations to the neuraminidase
The effective rate of treatment is as follows:
table 6 shows the effectiveness of treatment of 110 subjects after 28d of the stock solution of the pharmaceutical composition in use.
Table 6 Chinese medicinal composition stock solution treatment effectiveness
Test group III
1 object and method
1.1 subjects 40 female subjects, aged 25-50 years, were selected in Dongguan Huang Gusheng, traditional Chinese medicine hospitals. All subjects had received notification and signed informed consent.
1.2 inclusion criteria
(1) The skin photo-biological type is type II or III, and mainly uses indoor activities, can strictly comply with experimental requirements, and signs volunteer informed consent.
(2) Women aged 25-50 years;
(3) The course of the disease is more than or equal to 1 and less than or equal to 36 months;
(4) Skin patients with facial inflammation, eczema, psoriasis and the like which influence the observation of curative effect;
(5) No history of insolation prior to treatment;
(6) No treatment was received within 28d prior to participation in the subject study;
1.3 exclusion criteria
(1) Male patients;
(2) Patients older than 25 years old and older than 50 years old;
(3) Pregnant or lactating women:
(4) Patients with severe endocrine disease;
(5) Has serious primary diseases of cardiovascular, renal, hepatic, hematopoietic systems and the like;
(6) There are breast enlargement, mass and proliferative diseases;
(7) Patients with active infectious diseases;
(8) Patients with mental illness and severe neurosis can not cooperate with the therapeutic person.
1.4 method
40 subjects were selected and after facial cleansing in the morning and evening, the whole face was continuously used for 28 days with 2 times/d of the stock solution of the Chinese medicinal composition prepared in example 1.
1.5 evaluation of efficacy
Subjective evaluation of the subjects: after 28d, subjective evaluation was performed, and the content included in the subjective evaluation content included improvement in 4 aspects of clear, level, mottle, and acne marks. The evaluation criteria are classified into three classes of significant improvement, some improvement, and no improvement.
1.6, in the process of product safety evaluation and re-diagnosis, adverse reaction evaluation is carried out on the subjects, and the subjects are inquired whether the faces of the subjects have symptoms such as erythema, tingling and the like, if the symptoms appear, the correlation between the test products and the test products is evaluated through a patch test.
1.7 statistical analysis the above indicators were analyzed using SPSS19.0 software, t-test was used, andthe detection result is expressed as a ratio (%), and P is expressed as a ratio (%)<0.05 is a significant difference, there isStatistical significance.
2 results
Subject subjective evaluation after 28d, subjects were subjected to subjective evaluation, and the content included improvement in 4 aspects of clear, level, mottle, and acne marks, as detailed in table 3.
Table 7 subjective evaluation of subject (n)
In the whole research process of product safety evaluation, the subjects do not have adverse reactions, and the traditional Chinese medicine composition stock solution is proved to have higher safety.
Conclusion 3
Tyrosinase is a key enzyme in the melanin production process, and is the most important molecular target for researching melanin. The melanin inhibitor is synthesized by melanocytes only, so that the melanin inhibitor designed by taking tyrosinase as a target point has no obvious toxic or side effect on human bodies, and the early-stage melanin inhibition strategy is also mostly concentrated on tyrosinase. The melanin inhibitor has remarkable whitening effect, so that the melanin inhibitor becomes the first choice of the most basic components in cosmetics.
The results of this test showed that 87.5% of subjects considered improved skin clarity, 82.5% of subjects considered more even skin, 72.5% of subjects considered less mottle, and 65% of subjects considered effective in reducing acne marks, based on subjective evaluation of the subjects after 28d use. In the experimental process, 40 subjects have no adverse reaction, and the whitening and freckle-fading formula stock solution is proved to be capable of effectively inhibiting melanin from generating, homogenizing facial complexion, obviously improving color spots, acne marks and the like, enabling skin to be even and clear and high in use safety.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (8)
1. The traditional Chinese medicine composition for whitening skin and removing freckles is characterized by being prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of dittany bark extract or bletilla striata extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of dwarf lilyturf tuber extract, 2-4 parts of licorice root extract, and 2-4 parts of camellia extract or rose extract.
2. The traditional Chinese medicine composition according to claim 1, which is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of dittany bark extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of ophiopogon root extract, 2-4 parts of licorice root extract and 2-4 parts of camellia extract.
3. The traditional Chinese medicine composition according to claim 1, which is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of dittany bark extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of ophiopogon root extract, 2-4 parts of licorice root extract and 2-4 parts of rose extract.
4. The traditional Chinese medicine composition according to claim 1, which is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of bletilla striata extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of ophiopogon root extract, 2-4 parts of licorice root extract and 2-4 parts of camellia extract.
5. The traditional Chinese medicine composition according to claim 1, which is prepared from the following raw materials in parts by weight:
8-10 parts of ginseng extract, 8-10 parts of bletilla striata extract, 6-8 parts of chamomile extract, 4-6 parts of lucid ganoderma extract, 4-6 parts of pseudo-ginseng extract, 4-6 parts of saffron extract, 4-6 parts of hairyvein agrimony extract, 4-6 parts of ophiopogon root extract, 2-4 parts of licorice root extract and 2-4 parts of rose extract.
6. The use of the traditional Chinese medicine composition according to any one of claims 1-5 in preparing cosmetics for whitening skin and removing freckles.
7. The use according to claim 6, wherein the cosmetic comprises one or more of an emulsion, a cream, an essence, a foundation.
8. The use according to claim 6, wherein the cosmetic is for cleansing, isolating or screening the skin.
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